CN105768091A - Oat extract with constipation improving function as well as preparation method and application thereof - Google Patents
Oat extract with constipation improving function as well as preparation method and application thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 27
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an oat extract with a constipation improving function as well as a preparation method and an application thereof. The preparation method comprises the following concrete steps: mixing oat bran with water 8-12 times the weight of the oat bran, heating to 80-90 DEG C, maintaining the temperature for 1-2 hours, filtering to remove precipitates, cooling to 45-55 DEG C, adding cellulose, hydrolyzing for 1-3 hours, heating to 90-95 DEG C and maintaining the temperature for 10-15 minutes to kill the enzyme, cooling to the room temperature and obtaining the oat extract. The preparation method disclosed by the invention has the advantages that an organic reagent is not needed; the extraction method and the separation method are simple and convenient; and the obtained products are good in biological activity. Proved by human experimentations of volunteers, through the ingestion of 5-10 grams of the oat extract disclosed by the invention before meals, the constipation status can be obviously improved and the regular bowel habit is regained.
Description
Technical field
The present invention relates to the preparation method and application field of oat product, be specifically related to a kind of have improve purgative function
Oat extract and preparation method and application.
Background technology
It is solid that Herba bromi japonici (Avena sativa) has reduction serum cholesterol, triglyceride and serum low-density LP gallbladder
The important alimentary health-care functions such as alcohol level, and the water soluble dietary fiber that its health care is mainly attributed in oat grain-
Beta glucan.Avenabeta glucosan (Oat β-glucan) is with Herba bromi japonici or Herba bromi japonici bran as raw material, through hydrolyzing, extract, precipitating, dry
The white that the techniques such as dry, sterilizing are made, to buff powder, belongs to the structural non-starch polysaccharides(nsp) in plant cell wall.Herba bromi japonici β-
Glucosan is a kind of structural non-starch polysaccharides(nsp) being present in oat cell wall, within 2014, is regarded as newly providing by health ministry
One of derived food.Avenabeta glucosan has blood fat reducing, blood pressure lowering, the increment of promotion probiotic bacteria, prevents colon cancer, loses weight, anti-tired
Many physiologically actives such as labor, raising immunity.Avenabeta glucosan molecular weight is typically between 60,000-200 ten thousand dalton.Its knot
The D type glucose polymer that structure is mainly formed with β-(1,3)/β-(Isosorbide-5-Nitrae) glycosidic bond link of mixing, β-(1,3) sugar in molecule
Glycosidic bond often individualism, β-(Isosorbide-5-Nitrae) glycosidic bond then major part exists in the way of two greater numbers adjoin.Herba bromi japonici β-Portugal gathers
Sugar water solublity and water-insoluble two kinds, water solublity accounts for 50%～70%.Highly purified avenabeta glucosan is tasteless, slightly
The white of Herba bromi japonici local flavor, to buff powder, insoluble in ethanol, has stronger retentiveness, to temperature and pH stable.In recent years
Coming, Chinese scholars is a large amount of to having done of the aspects such as the extraction of avenabeta glucosan, structure, physicochemical property
Research.
Within 2006, disclose the preparation method (ZL 200610097431.X) of a kind of efficient prebiotics Herba bromi japonici Β-glucosan,
This patent, with Herba bromi japonici bran as raw material, size-reduced, microwave radiation exaraction, adds amylase and saccharifying enzyme process, isoelectric precipitation, centrifugal
Precipitation is collected in separation, supernatant concentration, ethanol precipitation, centrifugation, the redissolution that adds water, Β-glucanase hydrolyze, spray dried
Obtained after journey having significant promotion intestinal and faecal bifidobacteria, lactobacillus increment effect efficient prebiotics Herba bromi japonici Β-
Glucan product.Within 2010, Beijing Technology and Business University discloses a kind of oat extract and preparation method and application
(ZL201010130697.6).This oat extract is prepared by the method comprising the following steps: 1) by oat malt
It is added to the water, and regulates pH value to 9-11, heat treated 1-3 hour at 70-80 DEG C;2) liquid after heat treatment is cooled down,
Centrifugal, collect supernatant;The pH value regulating described supernatant is 5-6, then adds amylase in described supernatant and carries out enzyme
Solve reaction, after reaction terminates, sucking filtration, collect sucking filtration liquid, described sucking filtration liquid is carried out enzyme denaturing process, i.e. obtain described Herba bromi japonici and extract
Thing.In the same year, Beijing Technology and Business University also discloses a kind of oat extract and preparation method and application
(ZL201010180883.0).This preparation method comprises the steps: 1) with protease, bran of Fagopyrum esculentum Moench is carried out enzymolysis, will obtain
Product be denoted as protease hydrolyzed product;2) with amylase, described protease hydrolyzed product is carried out enzymolysis, the product that will obtain
Centrifugal, take supernatant, i.e. obtain oat extract.Within 2012, Wuxi Deguan Biotechnology Co., Ltd. provides a kind of extraction swallow
The method of wheat beta glucan, comprises the steps: that (1), by the stirring that adds water after Herba bromi japonici bran pulverizing, regulates pH6-7；(2) high temperature is added
α-amylase processes the starch in hydrolysis bran of Fagopyrum esculentum Moench at 85-90 DEG C；(3) add glucoamylase to process at 55-60 DEG C,
Filter, obtain supernatant liquid；(4) using molecular cut off is the ultrafiltration membrance filter of 50000Da-100000Da, obtains β-Portugal and gathers
Sugar juice；(5) jet drying obtains avenabeta glucosan product.The method of avenabeta glucosan is extracted in this invention, it is not necessary to have
Machine reagent, extraction separation method is easy, and gained beta glucan product purity is high, reaches more than 80%.Within 2013, large life is won in Nantong
Thing Engineering Co., Ltd discloses the extracting method (201310083864.X) of a kind of oat beta-glucan, and oatmeal is broken into Herba bromi japonici
Wheat bran, bran of Fagopyrum esculentum Moench clean in room temperature alkalescence deionized water after centrifuge dripping；The bran of Fagopyrum esculentum Moench of drying is joined deionization
In water, filter-cloth filtering after stirring, collect filtrate；Regathering filtrate after filtrate centrifugation, recycle silicon diatomaceous earth plate-and-frame filtration obtains
Filtrate, obtains filtrate with 8000rpm centrifugation the most again；The filtrate of isolated is added low temperature alpha-amylase, adjusts pH value
4.5-6.5, is warming up to 100 DEG C after stirring, stir 1 hour enzyme denaturing, uses kieselguhr plate-and-frame filtration, obtains supernatant liquid；Use
Ultrafiltration membrance filter, collecting molecular weight is the trapped fluid of 50000Da-100000Da, obtains oat beta-glucan solution, reconcentration, multiple
Molten and refined.Within 2015, Guangzhou Zhong Kang Food Co., Ltd provides the preparation method of a kind of avenabeta glucosan without anaphylactogen
(201510115883.5), comprise the following steps: 1) microwave drying；2) carbon dioxide supercritical fluid extraction；3) amylase enzymolysis；4)
Saccharifying enzyme enzymolysis；5) alkaline extraction；6) isoelectric point precipitates albumen；7) supernatant is collected in centrifugation；8) protease hydrolyzed；9) flocculation
Precipitation；10) supernatant is collected in centrifugation；11) membrance separation；12) it is concentrated in vacuo；13) alcohol precipitation；14) centrifugal collecting precipitation；
15) vacuum drying, should make to have reached high-purity without anaphylactogen avenabeta glucosan from the method for Herba bromi japonici bran extraction avenabeta glucosan
Spend, have the effect of specified molecular weight.University of Fuzhou in 2015 provides and a kind of utilizes fermentation method to extract avenabeta glucosan
Method (201510298282.2).The method comprises the following steps: 1) oat grain grinds and sieves, and takes the upper wheat bran of sieve and water-bath is returned
Stream enzyme denaturing；2) preparation of mycotic spore suspension；3) inoculation mycotic spore suspension fermentation；4) hot water extraction Herba bromi japonici β-Portugal gathers
Sugar；5) centrifuging and taking supernatant；6) concentrate, ethanol deposition and purification；7) water-bath is redissolved；8) lyophilization, obtain the β of purification-
Glucan product.The inventive method is simple to operate, it is not necessary to use enzyme preparation, low cost, can reach during the fermentation purification β-
The purpose of glucosan.
Foregoing invention and document are all not directed to the preparation method with the oat extract improving constipation effect.
Summary of the invention
It is an object of the invention to overcome deficiencies of the prior art, it is provided that a kind of have improve purgative function
Oat extract and preparation method and application.
The purpose of the present invention is achieved through the following technical solutions.
The preparation method of a kind of oat extract, the method preparation process is: by bran of Fagopyrum esculentum Moench and 6-12 times of bran of Fagopyrum esculentum Moench
The water mixing of weight, is heated to 80-90 DEG C and keeps 1-2h, filter and remove precipitation, after being cooled to 45-55 DEG C, adds cellulase
Hydrolysis 1-3h, is warming up to 90-95 DEG C of insulation 10-15min enzyme denaturing, filters and removes supernatant, be spray-dried after being cooled to room temperature,
Oat extract.
Further, described cellulase is neutral cellulase.
Further, the addition of described cellulase is the 0.3-0.8% of bran of Fagopyrum esculentum Moench weight.
Further, the molecular weight of described oat extract is more than 1000kDa.
Further, in described oat extract, the content of Herba bromi japonici beta glucan is more than 25%, and protein content is more than 20%,
Fat content is less than 5%.
What above-described preparation method prepared a kind of has the oat extract improving purgative function.
The application in preparing the health product and medicine improving constipation of the above-described oat extract.
The present invention compared with prior art, has the advantage that
1) preparation method of the present invention need not organic reagent, under the synergism of the Parameter Conditions limited, extracts and separates
Method is easy, and products obtained therefrom biological activity is good.
2) human volunteer's experiment shows, takes in oat extract prepared by the 5-10g present invention ante cibum and can obviously improve just
Secret state, returns rule bowl evacuation habit.
Detailed description of the invention
The present invention be will be clarified in more detail by following embodiment.
Various Methods for Determing Different Proteins: National Standard of the People's Republic of China GB 5009.5 2010
Determination of fat method: National Standard of the People's Republic of China GB/T5009.6-2003
Herba bromi japonici beta glucan content assaying method: congo red method
Embodiment 1
The water of 1000g bran of Fagopyrum esculentum Moench and 12 times of bran of Fagopyrum esculentum Moench weight is mixed, is heated to 90 DEG C and keeps 1h, filter and remove precipitation,
After being cooled to 55 DEG C, add 0.8% neutral cellulase hydrolysis 1h of bran of Fagopyrum esculentum Moench weight, be warming up to 95 DEG C of insulation 10min and go out
Enzyme, filters after being cooled to room temperature and removes supernatant, be spray-dried to obtain oat extract 1.
Testing result shows, in oat extract 1, the content of Herba bromi japonici beta glucan is 30.1%, and protein content is
24.2%, fat content is 3.5%.
Control sample:
The water of 1kg bran of Fagopyrum esculentum Moench and 12 times of bran of Fagopyrum esculentum Moench weight is mixed, is heated to 90 DEG C and keeps 1h, filter and remove precipitation, cold
But to after 55 DEG C, it is warming up to 95 DEG C of insulation 10min enzyme denaturing, filters after being cooled to room temperature and remove supernatant, be spray-dried to obtain Herba bromi japonici
Extract control sample.
Testing result shows, in oat extract control sample, the content of Herba bromi japonici beta glucan is 28.9%, and protein content is
24.0%, fat content is 3.3%.
The volunteer of the present invention is made up of 12 25-70 year women and 10 30-60 year male, and its average defecation time is
3-5 days once.Take in 5g oat extract before volunteer's lunch every day and supper, observe the change of its bowl evacuation habit, be shown in Table 1.
Table 1
| 24h | 48h | 72h | 96h | |
| The defecation number of oat extract 1 | 15 | 18 | 20 | 22 |
| The defecation number of oat extract control sample | 6 | 10 | 19 | 20 |
From table 1, volunteer, after taking in oat extract prepared by the employing present invention, exceedes the will of more than half (15 people)
Hope person starts defecation in 24h, and 18 people start defecation in 48h.And oat extract control sample only 6 people starts row in 24h
Just, 10 people start defecation in 48h.
Embodiment 2
The water of 10kg bran of Fagopyrum esculentum Moench and 6 times of bran of Fagopyrum esculentum Moench weight is mixed, is heated to 80 DEG C and keeps 2h, filter and remove precipitation, cold
But to after 45 DEG C, add 0.3% neutral cellulase hydrolysis 1h of bran of Fagopyrum esculentum Moench weight, be warming up to 90 DEG C of insulation 15min enzyme denaturing,
Filter after being cooled to room temperature and remove supernatant, be spray-dried to obtain oat extract 2.
Testing result shows, in oat extract 2, the content of Herba bromi japonici beta glucan is 28.4%, and protein content is
22.8%, fat content is 2.9%.
The water of 10kg bran of Fagopyrum esculentum Moench and 6 times of bran of Fagopyrum esculentum Moench weight is mixed, is heated to 80 DEG C of guarantors
Hold 2h, filter and remove precipitation, after being cooled to 45 DEG C, be warming up to 90 DEG C of insulation 15min enzyme denaturing, cross after being cooled to room temperature and filter off
Except supernatant, it is spray-dried to obtain oat extract control sample 2.
Volunteer is made up of 12 25-70 year women and 10 30-60 year male, and its average defecation time is 3-5 days one
Secondary.Take in 8g oat extract before volunteer's lunch every day and supper, observe the change of its bowl evacuation habit, be shown in Table 1.
Table 2
| 24h | 48h | 72h | 96h | |
| The defecation number of oat extract 2 | 17 | 20 | 22 | -- |
| The defecation number of oat extract control sample 2 | 6 | 12 | 17 | 20 |
From table 2, volunteer, after taking in oat extract prepared by the employing present invention, exceedes the will of more than half (17 people)
Hope person starts defecation in 24h, and 20 people start defecation in 48h.And oat extract control sample only 6 people starts row in 24h
Just, 12 people start defecation in 48h.
Embodiment 3
The water of 50kg bran of Fagopyrum esculentum Moench and 10 times of bran of Fagopyrum esculentum Moench weight is mixed, is heated to 92 DEG C and keeps 1.5h, filter and remove precipitation,
After being cooled to 50 DEG C, add 0.6% neutral cellulase hydrolysis 2h of bran of Fagopyrum esculentum Moench weight, be warming up to 95 DEG C of insulation 10min and go out
Enzyme, filters after being cooled to room temperature and removes supernatant, be spray-dried to obtain oat extract 3.
Testing result shows, in oat extract 3, the content of Herba bromi japonici beta glucan is 30.4%, and protein content is
24.6%, fat content is 3.0%.
Visible, the oat extract that the present invention prepares can apply to prepare in the health product and the medicine that improve constipation.
Claims (7)
1. a preparation method with the oat extract improving purgative function, it is characterised in that the method preparation process is:
The water of bran of Fagopyrum esculentum Moench with 6-12 times of bran of Fagopyrum esculentum Moench weight is mixed, is heated to 80-90 DEG C and keeps 1-2h, filter and remove precipitation, cold
But to after 45-55 DEG C, add cellulase hydrolysis 1-3h, be warming up to 90-95 DEG C of insulation 10-15min enzyme denaturing, after being cooled to room temperature
Filter and remove supernatant, be spray-dried, obtain oat extract.
Preparation method the most according to claim 1, is characterized in that described cellulase is neutral cellulase.
Preparation method the most according to claim 1, is characterized in that the addition of described cellulase is bran of Fagopyrum esculentum Moench weight
0.3-0.8%.
Preparation method the most according to claim 1, is characterized in that the molecular weight of described oat extract is more than 1000kDa.
Preparation method the most according to claim 1, is characterized in that in described oat extract, the content of Herba bromi japonici beta glucan is big
In 25%, protein content is more than 20%, and fat content is less than 5%.
6. by the preparation method described in any one of claim 1-5 prepare a kind of have improve purgative function Herba bromi japonici extract
Thing.
7. the application in preparing the health product and medicine improving constipation of the oat extract described in claim 6.
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Citations (5)
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|---|---|---|---|---|
| CN1235552A (en) * | 1996-09-25 | 1999-11-17 | 格雷斯林克有限公司 | Beta-glucan products and extration from cereals thereof |
| KR20080041784A (en) * | 2006-11-08 | 2008-05-14 | 씨제이제일제당 (주) | A method for making special beta-glucan by hydrolyzing crude beta-glucan |
| CN101602816A (en) * | 2009-06-18 | 2009-12-16 | 广东省食品工业研究所 | A kind of method of from the cereal bran, extracting high-purity water dissolubility beta-glucan |
| CN102585030A (en) * | 2011-12-23 | 2012-07-18 | 西藏天麦力健康品有限公司 | Method for extracting beta-glucan by using cereal |
| CN103204952A (en) * | 2013-03-18 | 2013-07-17 | 南通博硕生物工程有限公司 | Oat glucan extraction method |
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2016
- 2016-04-01 CN CN201610198943.9A patent/CN105768091A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1235552A (en) * | 1996-09-25 | 1999-11-17 | 格雷斯林克有限公司 | Beta-glucan products and extration from cereals thereof |
| KR20080041784A (en) * | 2006-11-08 | 2008-05-14 | 씨제이제일제당 (주) | A method for making special beta-glucan by hydrolyzing crude beta-glucan |
| CN101602816A (en) * | 2009-06-18 | 2009-12-16 | 广东省食品工业研究所 | A kind of method of from the cereal bran, extracting high-purity water dissolubility beta-glucan |
| CN102585030A (en) * | 2011-12-23 | 2012-07-18 | 西藏天麦力健康品有限公司 | Method for extracting beta-glucan by using cereal |
| CN103204952A (en) * | 2013-03-18 | 2013-07-17 | 南通博硕生物工程有限公司 | Oat glucan extraction method |
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