CN105767018A - Microbial pesticide for orange pest and disease control and preparation method thereof - Google Patents
Microbial pesticide for orange pest and disease control and preparation method thereof Download PDFInfo
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- CN105767018A CN105767018A CN201610276083.6A CN201610276083A CN105767018A CN 105767018 A CN105767018 A CN 105767018A CN 201610276083 A CN201610276083 A CN 201610276083A CN 105767018 A CN105767018 A CN 105767018A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N53/00—Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The invention provides microbial pesticide for orange pest and disease control. The microbial pesticide comprises, by weight, 12-18 parts of permethrin, 13-19 parts of cosolvent, 11-17 parts of emulgator, 6-12 parts of antifreezing agent, 1-3 parts of defoamer, 27-35 parts of water, 2-4 parts of forsythia extract, 1-3 parts of dioctyl divinyltriamino glycine, 1-3 parts of camptothecin and 1-3 parts of nucleic acid. The invention further provides a preparation method of the microbial pesticide for orange pest and disease control. The prepared microbial pesticide has good control effect in orange pests and diseases and has excellent control and resistance effect in orange plant and orange germs; meanwhile, yield of oranges is increased obviously, and juice yield of the oranges is increased significantly as well.
Description
Technical field
The invention belongs to preparation technique of pesticide field, be specifically related to a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage and preparation method thereof.
Background technology
Microbial pesticide, including agricultural antibiotic and alive microbial agrochemical.Disease, worm, grass, the plague of rats and the promotion plant growth harmed the crops for utilizing microorganism or its metabolite to prevent and treat.It include with bacterium control worm, fungus treatment, with bacterium weeding etc..It is strong that this kind of pesticide has selectivity, to people, animal, crops and natural environment safety, does not injure natural enemy, is not likely to produce the features such as resistance.These microbial pesticides include antibacterial, fungus, virus or its metabolite, for instance Bacillus thuringiensis, muscardine, nucleopolyhedrosis virus, jingganmycin, C type bacillus botulinus extracellular toxin etc..Along with the requirement that people are increasingly higher to environmental conservation, microbial pesticide is undoubtedly one of developing direction of pesticide from now on.
Fructus Citri junoris is the fruit of Rutaceae citrus plant orange, is also known as yellow fruit, mandarin orange, Jin Huan, Liu Ding.Fructus Citri junoris is a kind of hesperidium aurantium, is the Hybrid of Fructus Citri grandis and Fructus Citri tangerinae.Fructus Citri junoris edible nourishing is worth in using value high, but Fructus Citri junoris pest and disease damage is abnormal serious, brings great puzzlement to orchard workers.At present, existing microbial pesticide is poor for the prevention effect of Fructus Citri junoris pest and disease damage, causes Fructus Citri junoris Yield and quality to be affected by strong influence.Such as:
1, existing microbial pesticide is general to the prevention effect of Fructus Citri junoris common insect pests, and specific aim is not strong；
2, existing microbial pesticide can not produce Fructus Citri junoris easy infection pathogenic bacteria to suppress, opposing effect, make that Fructus Citri junoris is antibacterial, antibacterial effect is poor；
3, existing microbial pesticide is when carrying out the Fructus Citri junoris prevention and control of plant diseases, pest control, and the yield of Fructus Citri junoris reduces to some extent, and the crushing juice rate of Fructus Citri junoris also decreases.
Summary of the invention
For solving the technical problem existed in prior art, the present invention provides a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage and preparation method thereof, to realize following goal of the invention:
1, microbial pesticide prepared by the present invention is good to the prevention effect of Fructus Citri junoris insect；
2, microbial pesticide prepared by the present invention produce Fructus Citri junoris pathogenic bacteria to suppress, resistivity, antibacterial, antibacterial effect is good；
3, microbial pesticide prepared by the present invention is while carrying out the Fructus Citri junoris prevention and control of plant diseases, pest control and Anti-bacterium growth, and the yield of Fructus Citri junoris is significantly improved, and the crushing juice rate of Fructus Citri junoris also significantly improves.
For solving above-mentioned technical problem, the technical scheme that the present invention takes is as follows:
A kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage, described microbial pesticide includes following components: Permethrin, cosolvent, emulsifying agent, antifreezing agent, defoamer, water, Fructus Forsythiae extract, octicin solution, camptothecine, nucleic acid.
The following is the further improvement to technique scheme:
The component of described microbial pesticide, with parts by weight, content is:
Permethrin 12～18 parts, cosolvent 13～19 parts, emulsifying agent 11～17 parts, antifreezing agent 6～12 parts, defoamer 1～3 part, 27～35 parts of water, Fructus Forsythiae extract 2～4 parts, octicin solution 1～3 part, camptothecine 1～3 part, nucleic acid 1～3 part.
The component of described microbial pesticide, with parts by weight, content is:
Permethrin 15 parts, cosolvent 16 parts, emulsifying agent 14 parts, antifreezing agent 9 parts, defoamer 2 parts, 30 parts of water, Fructus Forsythiae extract 3 parts, octicin solution 2 parts, camptothecine 2 parts, nucleic acid 2 parts.
Described cosolvent is N-Methyl pyrrolidone.
Described emulsifying agent is phenethyl fluosite polyoxyethyl ether.
Described antifreezing agent is glycerol.
Described defoamer is C7 aliphatic alcohols compound.
Described Fructus Forsythiae extract includes: phillyrin and summary and discussion；Described Determination of forsythin is 5%, summary and discussion 2%.
The preparation method of a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage, comprises the following steps:
(1) the weight ratio raw material of above-described embodiment 1～3 is taken；
(2) first Permethrin is mixed homogeneously under mechanical stirring with N-Methyl pyrrolidone, form oil phase；
(3) phenethyl fluosite polyoxyethyl ether, glycerol, C7 aliphatic alcohols compound, water, Fructus Forsythiae extract, octicin solution, camptothecine and nucleic acid are put in emulsion dispersion still, and under the rotating speed of 10000 revs/min, start stirring；
(4) oil phase is put in emulsion dispersion still, stir under the rotating speed of 1200 revs/min, stir 20 minutes；
(5) microbial pesticide of the present invention is namely obtained.
The extracting method of described Fructus Forsythiae extract comprises the following steps:
(1) taking Fructus Forsythiae, add water and carry out decocting 2 hours, leaching Fructus Forsythiae liquid, Fructus Forsythiae slag adds water decoction 1 hour, leaching Fructus Forsythiae liquid, abandons medicinal residues；Merge twice Fructus Forsythiae decocting liquid, Fructus Forsythiae decocting liquid is concentrated into relative density 1.20, be cooled to room temperature and obtain Fructus Forsythiae decoction liquor；
(2) Fructus Forsythiae decoction liquor is under agitation added alcoholic solution, alcohol content is made to reach 80%, cold preservation more than 12 hours under 10 DEG C of conditions, filter, Fructus Forsythiae filtrate reclaims alcoholic solution to Fructus Forsythiae filtrate without alcohol taste, and heating is concentrated into relative density 1.10, after being cooled to room temperature, cold preservation more than 24 hours under 10 DEG C of conditions, filter to obtain purification Fructus Forsythiae liquid；
(3) purification Fructus Forsythiae liquid extracts with appropriate water saturation alcoholic solution, merges repeatedly extract, and recovered under reduced pressure alcoholic solution obtains thick paste, thick paste is dissolved in water, cold preservation more than 24 hours under 10 DEG C of conditions, filters, Fructus Forsythiae filtrate is concentrated into thick paste, less than 80 DEG C drying under reduced pressure, obtains Fructus Forsythiae extract.
Compared with prior art, the invention have the benefit that
1, microbial pesticide prepared by the present invention is good to the prevention effect of Fructus Citri junoris insect, after dispenser 45 days, the insect common to Fructus Citri junoris: red spider, rust mite, leaf miner, aleyrodid, acarid prevention effect respectively reach 96.6～98.9%, 95.9～98.2%, 96.5～99.0%, 95.4～98.9%, 96.2～98.2%；
2, Fructus Citri junoris pathogenic bacteria is produced suppression, resistivity by microbial pesticide prepared by the present invention, antibacterial, antibacterial effect is good, after dispenser 30 days, the pathogenic bacteria common to Fructus Citri junoris: streptococcus, vibrio cholera, staphylococcus, bacillus pyocyaneus suppression, resist effective, respectively reach 95.4～98.8%, 96.3～98.5%, 95.9～98.7%, 96.4～98.9%；
3, microbial pesticide prepared by the present invention is while carrying out the Fructus Citri junoris prevention and control of plant diseases, pest control and Anti-bacterium growth, the yield of Fructus Citri junoris is significantly improved, and the crushing juice rate of Fructus Citri junoris also significantly improves, the per mu yield of Fructus Citri junoris reaches 4500～6000 jin, and the crushing juice rate of Fructus Citri junoris reaches 45～60%.
Detailed description of the invention
Below by way of specific embodiment, the present invention is described, but the present invention is not merely defined in these embodiments.
Embodiment 1 one kinds prevents and treats the microbial pesticide of Fructus Citri junoris pest and disease damage
Described microbial pesticide includes the component of following weight portion:
Permethrin 12 parts, cosolvent 13 parts, emulsifying agent 11 parts, antifreezing agent 6 parts, defoamer 1 part, 27 parts of water, Fructus Forsythiae extract 2 parts, octicin solution 1 part, camptothecine 1 part, nucleic acid 1 part；
Described cosolvent is N-Methyl pyrrolidone；
Described emulsifying agent is phenethyl fluosite polyoxyethyl ether；
Described antifreezing agent is glycerol；
Described defoamer is C7 aliphatic alcohols compound；
Described Fructus Forsythiae extract includes: phillyrin and summary and discussion；Described Determination of forsythin is 5%, summary and discussion 2%.
Embodiment 2 one kinds prevents and treats the microbial pesticide of Fructus Citri junoris pest and disease damage
Described microbial pesticide includes the component of following weight portion:
Permethrin 15 parts, cosolvent 16 parts, emulsifying agent 14 parts, antifreezing agent 9 parts, defoamer 2 parts, 30 parts of water, Fructus Forsythiae extract 3 parts, octicin solution 2 parts, camptothecine 2 parts, nucleic acid 2 parts；
Described cosolvent is N-Methyl pyrrolidone；
Described emulsifying agent is phenethyl fluosite polyoxyethyl ether；
Described antifreezing agent is glycerol；
Described defoamer is C7 aliphatic alcohols compound；
Described Fructus Forsythiae extract includes: phillyrin and summary and discussion；Described Determination of forsythin is 5%, summary and discussion 2%.
Embodiment 3 one kinds prevents and treats the microbial pesticide of Fructus Citri junoris pest and disease damage
Permethrin 18 parts, cosolvent 19 parts, emulsifying agent 17 parts, antifreezing agent 12 parts, defoamer 3 parts, 35 parts of water, Fructus Forsythiae extract 4 parts, octicin solution 3 parts, camptothecine 3 parts, nucleic acid 3 parts；
Described cosolvent is N-Methyl pyrrolidone；
Described emulsifying agent is phenethyl fluosite polyoxyethyl ether；
Described antifreezing agent is glycerol；
Described defoamer is C7 aliphatic alcohols compound；
Described Fructus Forsythiae extract includes: phillyrin and summary and discussion；Described Determination of forsythin is 5%, summary and discussion 2%.
The preparation method that embodiment 4 one kinds prevents and treats the microbial pesticide of Fructus Citri junoris pest and disease damage
The preparation method of a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage comprises the following steps:
(1) the weight ratio raw material of above-described embodiment 1～3 is taken；
(2) first Permethrin is mixed homogeneously under mechanical stirring with N-Methyl pyrrolidone, form oil phase；
(3) phenethyl fluosite polyoxyethyl ether, glycerol, C7 aliphatic alcohols compound, water, Fructus Forsythiae extract, octicin solution, camptothecine and nucleic acid are put in emulsion dispersion still, and under the rotating speed of 10000 revs/min, start stirring；
(4) oil phase is put in emulsion dispersion still, stir under the rotating speed of 1200 revs/min, stir 20 minutes；
(5) microbial pesticide of the present invention is namely obtained.
The extracting method of described Fructus Forsythiae extract comprises the following steps:
(1) taking Fructus Forsythiae, add water and carry out decocting 2 hours, leaching Fructus Forsythiae liquid, Fructus Forsythiae slag adds water decoction 1 hour, leaching Fructus Forsythiae liquid, abandons medicinal residues；Merge twice Fructus Forsythiae decocting liquid, Fructus Forsythiae decocting liquid is concentrated into relative density 1.20, be cooled to room temperature and obtain Fructus Forsythiae decoction liquor；
(2) Fructus Forsythiae decoction liquor is under agitation added alcoholic solution, alcohol content is made to reach 80%, cold preservation more than 12 hours under 10 DEG C of conditions, filter, Fructus Forsythiae filtrate reclaims alcoholic solution to Fructus Forsythiae filtrate without alcohol taste, and heating is concentrated into relative density 1.10, after being cooled to room temperature, cold preservation more than 24 hours under 10 DEG C of conditions, filter to obtain purification Fructus Forsythiae liquid；
(3) purification Fructus Forsythiae liquid extracts with appropriate water saturation alcoholic solution, merges repeatedly extract, and recovered under reduced pressure alcoholic solution obtains thick paste, thick paste is dissolved in water, cold preservation more than 24 hours under 10 DEG C of conditions, filters, Fructus Forsythiae filtrate is concentrated into thick paste, less than 80 DEG C drying under reduced pressure, obtains Fructus Forsythiae extract.
Percent in above-described embodiment, except specified otherwise, for mass percent.
Result detects:
(1) microbial pesticide prepared by the present invention is good to the prevention effect of Fructus Citri junoris insect, after dispenser 45 days, the insect common to Fructus Citri junoris: red spider, rust mite, leaf miner, aleyrodid, acarid prevention effect respectively reach 96.6～98.9%, 95.9～98.2%, 96.5～99.0%, 95.4～98.9%, 96.2～98.2%, specific targets are in Table 1；
Microbial pesticide prepared by table 1 present invention prevention effect to Fructus Citri junoris common insect pests
(2) Fructus Citri junoris pathogenic bacteria is produced suppression, resistivity by microbial pesticide prepared by the present invention, antibacterial, antibacterial effect is good, after dispenser 30 days, the pathogenic bacteria common to Fructus Citri junoris: streptococcus, vibrio cholera, staphylococcus, bacillus pyocyaneus suppression, resist effective, respectively reaching 95.4～98.8%, 96.3～98.5%, 95.9～98.7%, 96.4～98.9%, specific targets are in Table 2；
Microbial pesticide prepared by table 2 present invention is to the suppression of the common pathogenic bacteria of Fructus Citri junoris, opposing effect
(3) microbial pesticide prepared by the present invention is while carrying out the Fructus Citri junoris prevention and control of plant diseases, pest control and Anti-bacterium growth, the yield of Fructus Citri junoris is significantly improved, and the crushing juice rate of Fructus Citri junoris also significantly improves, and the per mu yield of Fructus Citri junoris reaches 4500～6000 jin, the crushing juice rate of Fructus Citri junoris reaches 45～60%, is specifically shown in table 3；
The per mu yield of table 3 Fructus Citri junoris and the content of pectin, fructose
Comparison: do not use microbial pesticide of the present invention, replaces microbial pesticide to spray with clear water.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention, although the present invention being described in detail with reference to previous embodiment, for a person skilled in the art, technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature carries out equivalent replacement.All within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (10)
1. the microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage, it is characterised in that: described microbial pesticide includes following components:
Permethrin, cosolvent, emulsifying agent, antifreezing agent, defoamer, water, Fructus Forsythiae extract, octicin solution, camptothecine, nucleic acid.
2. a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage according to claim 1, it is characterised in that: the component of described microbial pesticide, with parts by weight, content is:
Permethrin 12～18 parts, cosolvent 13～19 parts, emulsifying agent 11～17 parts, antifreezing agent 6～12 parts, defoamer 1～3 part, 27～35 parts of water, Fructus Forsythiae extract 2～4 parts, octicin solution 1～3 part, camptothecine 1～3 part, nucleic acid 1～3 part.
3. a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage according to claim 1, it is characterised in that: the component of described microbial pesticide, with parts by weight, content is:
Permethrin 15 parts, cosolvent 16 parts, emulsifying agent 14 parts, antifreezing agent 9 parts, defoamer 2 parts, 30 parts of water, Fructus Forsythiae extract 3 parts, octicin solution 2 parts, camptothecine 2 parts, nucleic acid 2 parts.
4. a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage according to claim 1, it is characterised in that: described cosolvent is N-Methyl pyrrolidone.
5. a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage according to claim 1, it is characterised in that: described emulsifying agent is phenethyl fluosite polyoxyethyl ether.
6. a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage according to claim 1, it is characterised in that: described antifreezing agent is glycerol.
7. a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage according to claim 1, it is characterised in that: described defoamer is C7 aliphatic alcohols compound.
8. a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage according to claim 1, it is characterised in that: described Fructus Forsythiae extract includes: phillyrin and summary and discussion；Described Determination of forsythin is 5%, summary and discussion 2%.
9. the preparation method of the microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage, it is characterised in that comprise the following steps:
(1) the weight ratio raw material of above-described embodiment 1～3 is taken；
(2) first Permethrin is mixed homogeneously under mechanical stirring with N-Methyl pyrrolidone, form oil phase；
(3) phenethyl fluosite polyoxyethyl ether, glycerol, C7 aliphatic alcohols compound, water, Fructus Forsythiae extract, octicin solution, camptothecine and nucleic acid are put in emulsion dispersion still, and under the rotating speed of 10000 revs/min, start stirring；
(4) oil phase is put in emulsion dispersion still, stir under the rotating speed of 1200 revs/min, stir 20 minutes；
(5) microbial pesticide of the present invention is namely obtained.
10. the preparation method of a kind of microbial pesticide preventing and treating Fructus Citri junoris pest and disease damage according to claim 9, it is characterised in that:
The extracting method of described Fructus Forsythiae extract comprises the following steps:
(1) taking Fructus Forsythiae, add water and carry out decocting 2 hours, leaching Fructus Forsythiae liquid, Fructus Forsythiae slag adds water decoction 1 hour, leaching Fructus Forsythiae liquid, abandons medicinal residues；Merge twice Fructus Forsythiae decocting liquid, Fructus Forsythiae decocting liquid is concentrated into relative density 1.20, be cooled to room temperature and obtain Fructus Forsythiae decoction liquor；
(2) Fructus Forsythiae decoction liquor is under agitation added alcoholic solution, alcohol content is made to reach 80%, cold preservation more than 12 hours under 10 DEG C of conditions, filter, Fructus Forsythiae filtrate reclaims alcoholic solution to Fructus Forsythiae filtrate without alcohol taste, and heating is concentrated into relative density 1.10, after being cooled to room temperature, cold preservation more than 24 hours under 10 DEG C of conditions, filter to obtain purification Fructus Forsythiae liquid；
(3) purification Fructus Forsythiae liquid extracts with appropriate water saturation alcoholic solution, merges repeatedly extract, and recovered under reduced pressure alcoholic solution obtains thick paste, thick paste is dissolved in water, cold preservation more than 24 hours under 10 DEG C of conditions, filters, Fructus Forsythiae filtrate is concentrated into thick paste, less than 80 DEG C drying under reduced pressure, obtains Fructus Forsythiae extract.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114304155A (en) * | 2021-12-09 | 2022-04-12 | 山东省农业科学院作物研究所 | Method for preventing and controlling sweet potato underground pests based on plant extracts |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101300976A (en) * | 2007-05-11 | 2008-11-12 | 增井吉晴 | Antibacterial deodorizer |
| CN101370941A (en) * | 2006-01-12 | 2009-02-18 | 德福根有限公司 | dsRNA as insect control agent |
| CN101570752A (en) * | 2009-04-27 | 2009-11-04 | 西北农林科技大学 | Preparation and application of small interfering RNA for preventing tobacco mosaic virus diseases |
| CN101959419A (en) * | 2008-01-10 | 2011-01-26 | 先锋国际良种公司 | Novel bacillus thuringiensis gene with coleopteran activity |
| CN102132703A (en) * | 2011-01-19 | 2011-07-27 | 江苏丰山集团有限公司 | Composite pesticide containing permethrin and emamectin benzoate |
| CN102453036A (en) * | 2010-10-27 | 2012-05-16 | 李红玉 | Camptothecin compound and preparation method and application in pesticide thereof |
| CN102675438A (en) * | 2011-03-18 | 2012-09-19 | 中国科学院上海生命科学研究院 | Anti-insect preparation and method based RNAi (ribonucleic acid interfere) technology |
-
2016
- 2016-04-29 CN CN201610276083.6A patent/CN105767018A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101370941A (en) * | 2006-01-12 | 2009-02-18 | 德福根有限公司 | dsRNA as insect control agent |
| CN101300976A (en) * | 2007-05-11 | 2008-11-12 | 增井吉晴 | Antibacterial deodorizer |
| CN101959419A (en) * | 2008-01-10 | 2011-01-26 | 先锋国际良种公司 | Novel bacillus thuringiensis gene with coleopteran activity |
| CN101570752A (en) * | 2009-04-27 | 2009-11-04 | 西北农林科技大学 | Preparation and application of small interfering RNA for preventing tobacco mosaic virus diseases |
| CN102453036A (en) * | 2010-10-27 | 2012-05-16 | 李红玉 | Camptothecin compound and preparation method and application in pesticide thereof |
| CN102132703A (en) * | 2011-01-19 | 2011-07-27 | 江苏丰山集团有限公司 | Composite pesticide containing permethrin and emamectin benzoate |
| CN102675438A (en) * | 2011-03-18 | 2012-09-19 | 中国科学院上海生命科学研究院 | Anti-insect preparation and method based RNAi (ribonucleic acid interfere) technology |
Non-Patent Citations (5)
| Title |
|---|
| IBRAHIM EL-SHESHENY: "Silencing Abnormal Wing Disc Gene of the Asian Citrus Psyllid, Diaphorina citri Disrupts Adult Wing Development and Increases Nymph Mortality", 《PLOS ONE》 * |
| 曹光明: "《中药浸提物生产工艺学》", 30 April 2009 * |
| 杨洪强: "《有机园艺》", 31 July 2005 * |
| 赵善欢: "《植物化学保护》", 31 January 2002 * |
| 赵陆华: "《中药高效液相色谱法应用》", 31 May 2005 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114304155A (en) * | 2021-12-09 | 2022-04-12 | 山东省农业科学院作物研究所 | Method for preventing and controlling sweet potato underground pests based on plant extracts |
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