CN105759049A - Protein chip for detecting rabies viruses - Google Patents
Protein chip for detecting rabies viruses Download PDFInfo
- Publication number
- CN105759049A CN105759049A CN201410807904.5A CN201410807904A CN105759049A CN 105759049 A CN105759049 A CN 105759049A CN 201410807904 A CN201410807904 A CN 201410807904A CN 105759049 A CN105759049 A CN 105759049A
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- China
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- rabies virus
- protein chip
- protein
- glass substrate
- coating
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a protein chip for detecting rabies viruses.The protein chip comprises a glass substrate, rabies virus receptor protein arrayed on the substrate and control point coating, wherein the glass substrate is modified by nickel, and the point coating comprises 16 rabies virus receptor protein detecting areas, 4 positive control areas and 4 negative control areas.The protein chip provides an economic, fast and sensitive method for rabies virus detection and is accurate and reliable in detection result.
Description
Technical field
The invention relates to protein chip field, particularly relates to a kind of protein chip detecting rabies virus.
Background technology
Protein chip is a kind of novel biochip, it is made up of the arrays of immobilized protein being fixed on variety classes supporting dielectric, in array, the position of fixed member and composition are known, translate with the probe on chip by the biomolecule of un-marked or labelling, then pass through specific scanning means to detect, result is analyzed and processed by computer, is fly another technology platform for life science after gene chip.Protein chip has the advantages such as high specificity, sensitivity is high, flux is high, reproducible, application is strong.Rabies virus is height neurotropic virus, rides and migrates to central nervous system from portal of entry.It is reported, have more than 70,000 people every year and die from rabies, and millions of the infecteds need to expose after treatment.Although being made that major progress in rabic prevention and control, but this disease having remained public health significant threat.
Summary of the invention
For above-mentioned deficiency, the invention the technical scheme is that
A kind of protein chip detecting rabies virus, the rabies virus receptor protein being distributed on substrate by glass substrate and array forms with the coating compareed, wherein, described glass substrate surface is modified through nickel, described some coating includes 16 rabies virus receptor protein detection zones, 4 positive control area, 4 negative control area.
Further, described some coating is circular, and diameter is 0.2mm, and spot printing interlamellar spacing is 0.6mm.
The invention has the advantage that and has the benefit effect that the detection that the invention is rabies virus provides a kind of economy, quick, sensitive method, and testing result is accurately and reliably.
Accompanying drawing explanation
Fig. 1 is a kind of protein chip schematic diagram detecting rabies virus
In figure: 1, positive control 2, rabies virus receptor protein 3, some coating 4, negative control
5, glass substrate
Detailed description of the invention
A kind of protein chip detecting rabies virus, the rabies virus receptor protein 2 being distributed on substrate by glass substrate 5 and array forms with the some coating 3 compareed, wherein, described glass substrate 5 surface is modified through nickel, and described some coating 3 includes 16 rabies virus receptor protein 2 detection zones, 4 positive control area, 4 negative control area, described some coating 3 is circular, and diameter is 0.2mm, and spot printing interlamellar spacing is 0.6mm.
The rabies virus protein chip that the invention provides can directly bring use, will add this protein chip incubation after serum-dilution to be measured, is subsequently adding two and resists.Result judges as follows: ' negative ' specimens does not develop the color, and is background colour (background colour refers to that the color of chip matrix, part between points are background), and colour developing is the positive, just can slightly judge positive power according to the depth degree of colour developing.
Above an embodiment of the invention is described in detail, but described content has been only the preferred embodiment of the invention, it is impossible to be considered the practical range for limiting the invention.All equalizations made according to the invention application range change and improvement etc., all should still belong within the patent covering scope of the invention.
Claims (2)
1. the protein chip detecting rabies virus, it is characterized in that: the rabies virus receptor protein being distributed on substrate by glass substrate and array forms with the some coating compareed, wherein, described glass substrate surface is modified through nickel, described some coating includes 16 rabies virus receptor protein detection zones, 4 positive control area, 4 negative control area.
2. a kind of protein chip detecting rabies virus according to claim 1, it is characterised in that: described some coating is circular, and diameter is 0.2mm, and spot printing interlamellar spacing is 0.6mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410807904.5A CN105759049A (en) | 2014-12-20 | 2014-12-20 | Protein chip for detecting rabies viruses |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410807904.5A CN105759049A (en) | 2014-12-20 | 2014-12-20 | Protein chip for detecting rabies viruses |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105759049A true CN105759049A (en) | 2016-07-13 |
Family
ID=56341414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410807904.5A Pending CN105759049A (en) | 2014-12-20 | 2014-12-20 | Protein chip for detecting rabies viruses |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105759049A (en) |
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2014
- 2014-12-20 CN CN201410807904.5A patent/CN105759049A/en active Pending
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160713 |