CN205262967U - Digit PCR analysis appearance - Google Patents
Digit PCR analysis appearance Download PDFInfo
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- CN205262967U CN205262967U CN201520863323.3U CN201520863323U CN205262967U CN 205262967 U CN205262967 U CN 205262967U CN 201520863323 U CN201520863323 U CN 201520863323U CN 205262967 U CN205262967 U CN 205262967U
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- 238000010222 PCR analysis Methods 0.000 title abstract 2
- 238000001914 filtration Methods 0.000 claims abstract description 26
- 238000012545 processing Methods 0.000 claims abstract description 13
- 239000011521 glass Substances 0.000 claims description 18
- 239000000758 substrate Substances 0.000 claims description 18
- 239000004065 semiconductor Substances 0.000 claims description 11
- 229910021419 crystalline silicon Inorganic materials 0.000 claims description 3
- 230000006698 induction Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 230000005693 optoelectronics Effects 0.000 claims description 3
- 229910021420 polycrystalline silicon Inorganic materials 0.000 claims description 3
- 229920005591 polysilicon Polymers 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 239000000975 dye Substances 0.000 abstract description 4
- 229920002521 macromolecule Polymers 0.000 abstract description 3
- 230000010365 information processing Effects 0.000 abstract 3
- 230000005622 photoelectricity Effects 0.000 abstract 3
- 239000010409 thin film Substances 0.000 abstract 3
- 230000005611 electricity Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 8
- 150000007523 nucleic acids Chemical class 0.000 description 8
- 102000039446 nucleic acids Human genes 0.000 description 8
- 238000001514 detection method Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 5
- 238000012797 qualification Methods 0.000 description 4
- 238000003753 real-time PCR Methods 0.000 description 4
- 238000011304 droplet digital PCR Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 150000002605 large molecules Chemical class 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 108700011259 MicroRNAs Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000007481 next generation sequencing Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model provides a digit PCR analysis appearance, including control and information processing module and respectively rather than light source, micropore sample array and the bigri photoelectricity thin film transistor array of electricity connection, micropore sample array sets up between light source and bigri photoelectricity thin film transistor array. The sample is arranged in the micropore sample room, control and information processing module control light source shine the sample, the dyestuff preliminary mixing and that combine to be in the same place in specific biomacromolecule is excited in the sample, send fluorescence, after filtering, bigri photoelectricity thin film transistor response that quilt and micropore sample room correspond turns into the signal of telecommunication, be that available light intensity is corresponding with the content of the specific macromolecule of every sample through control and information processing module processing, thereby can calculate biomacromolecule's concentration. The utility model has the advantages of it is small, with low costs.
Description
Technical field
The utility model relates to molecular Biological Detection field, more specifically, relates to a kind of digital pcr analyzer.
Background technology
Digital pcr is DigitalPCR(dPCR), it is a kind of nucleic acid molecules absolute quantitation measuring technology. different from traditional quantitative PCR (qPCR) technology, digital pcr adopts the mode of absolute quantitation, does not rely on calibration curve and sample for reference, the copy number of direct-detection target sequence. because dPCR has the sensitivity outstanding than qPCR and specificity and accuracy, dPCR technology has obtained development rapidly, this technology detects at denier sample of nucleic acid, the advantage that under complex background, rare mutation detects and expression fine difference qualification aspect is cashed out has obtained general accreditation, and it is in gene expression research, microRNA research, the qualification of genome copy number, cancer markers rare mutation detects, pathogenic microorganisms qualification, transgene component qualification, all many-sides such as NGS sequencing library accurate quantification and result verification have broad application prospects.
Common digital pcr instrument mainly adopts the technology of semiconductor chips changing for the faint detection technique of fluorescence of droplet with for the pH value in the micro-course of reaction of nucleic acid in the market. Microdrop technique is, before traditional pcr amplification, sample is carried out to droplet processing, the reaction system that is about to contain nucleic acid molecules is divided into the thousands of individual droplets of upgrading of receiving, wherein each droplet or containing nucleic acid target molecule to be checked, or contain one to several nucleic acid target molecules to be checked. After pcr amplification, one by one each droplet is detected. It is relatively large that the advantage of this method is to react quantity and detects the degree of depth; but its shortcoming is also very outstanding is that detection speed is very slow; the several hours often detection time of a reaction, and consider for protection droplet film, when reaction, the speed of variations in temperature is also limited. The technology of semiconductor chips is mainly the micro-fluidic method that adopts the popular research field of present analysis chemistry, nucleic acid solution is dispersed in the microreactor of chip and reacts, and detect. Its advantage is reaction and detects rapidly, similar with the common fluorescence quantitative PCR detection time. But because its reaction quantity that limits of chip technology has certain inferior position compared with microdrop technique.
As shown in Figure 1, the digital pcr analyzer based on fluoroscopic examination comprises sample block, optical module and detector module three parts. The general employing of its optical system is reflective, the exciting light that light source sends is radiated on the nucleic acid molecules that contains dyestuff after reflect focalization, the fluorescence of excited nucleus acid molecule enters CCD or cmos detector module after reflection, filtering subsequently, and the intensity signal of fluorescence goes on record and be processed. Such system needs complicated optical system conventionally, and therefore systems bulky, involves great expense.
Utility model content
The utility model is to overcome bulky described in above-mentioned prior art, and the defect involving great expense, provides the digital pcr that a kind of volume is little, cost is low analyzer.
For solving the problems of the technologies described above, the technical solution of the utility model is as follows:
A kind of digital pcr analyzer, comprise control and message processing module and the light source being electrically connected with it respectively, micropore sample array and bigrid photo tft array, described micropore sample array is arranged between light source and bigrid photo tft array, described micropore sample array comprises multiple micropores sample room, described bigrid photo tft array comprises multiple bigrid photo tfts, and described micropore sample room is corresponding one by one in vertical direction with bigrid photo tft.
In the preferred scheme of one, described light source, micropore sample array and bigrid photo tft array are arranged from top to bottom, described bigrid photo tft comprises glass substrate, dark grid, bottom dielectric layer, channel layer, source electrode, drain electrode, top dielectric, photogate and wave filtering layer, dark grid is arranged on glass substrate, bottom dielectric layer is arranged on glass substrate and covers dark grid, channel layer is arranged in bottom dielectric layer, source electrode and drain electrode are arranged on channel layer, top dielectric is arranged on semiconductor channel layer and covers source electrode and drain electrode, the photogate of transparent and electrically conductive is arranged in top dielectric, wave filtering layer is arranged in top dielectric and covers photogate, or wave filtering layer is arranged at photogate top.
In the preferred scheme of one, described light source, micropore sample array and bigrid photo tft array are arranged from top to bottom, described bigrid photo tft comprises glass substrate, dark grid, bottom dielectric layer, channel layer, source electrode, drain electrode, top dielectric, photogate and wave filtering layer, dark grid is arranged under glass substrate, bottom dielectric layer is arranged under glass substrate and covers dark grid, channel layer is arranged under bottom dielectric layer, source electrode and drain electrode are arranged under channel layer, top dielectric is arranged under semiconductor channel layer and covers source electrode and drain electrode, the photogate of transparent and electrically conductive is arranged under top dielectric, wave filtering layer is arranged under top dielectric and covers photogate, or wave filtering layer is arranged at photogate below.
In the preferred scheme of one, the material of described channel layer is non-crystalline silicon, organic semiconductor, polysilicon or the oxide with optoelectronic induction function.
In the preferred scheme of one, described light source is high-brightness LED or OLED.
In the preferred scheme of one, be made up of transparent material described micropore sample room, and fluorescence or exciting light are had to certain transmitance.
In the preferred scheme of one, the size of described micropore sample room is less than or equal to bigrid photo tft, to reduce optical crosstalk.
In the preferred scheme of one, described micropore sample room is microfluidic control device.
Compared with prior art, the beneficial effect of technical solutions of the utility model is: the utility model provides a kind of digital pcr analyzer, comprise control and message processing module and the light source being electrically connected with it respectively, micropore sample array and bigrid photo tft array, described micropore sample array is arranged between light source and bigrid photo tft array, described micropore sample array comprises multiple micropores sample room, described bigrid photo tft array comprises multiple bigrid photo tfts, described micropore sample room is corresponding one by one in vertical direction with bigrid photo tft. sample is arranged in micropore sample room, control and message processing module control light source irradiate sample, dyestuff that be pre-mixed in sample and that combine in the large molecule of particular organisms is stimulated, send fluorescence, after filtering, responded to by the bigrid photo tft corresponding with micropore sample room and be converted into the signal of telecommunication, through controlling, processing is that available light intensity is corresponding with the specific macromolecular content of each sample with message processing module, thereby can calculate the concentration of large biological molecule. the utlity model has the advantage that volume is little, cost is low.
Brief description of the drawings
Fig. 1 is the digital pcr analyzer schematic diagram based on fluoroscopic examination.
Fig. 2 is the schematic diagram of the utility model digital pcr analyzer.
Fig. 3 is that wave filtering layer is arranged at the bigrid photo tft schematic diagram in top dielectric and while covering photogate.
Fig. 4 is that wave filtering layer is arranged at the photogate bigrid photo tft schematic diagram in when top.
Fig. 5 is that wave filtering layer is arranged at the bigrid photo tft schematic diagram under top dielectric and while covering photogate.
Fig. 6 is that wave filtering layer is arranged at the photogate bigrid photo tft schematic diagram in when below.
Detailed description of the invention
Accompanying drawing, only for exemplary illustration, can not be interpreted as the restriction to this patent;
For better explanation the present embodiment, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product;
To those skilled in the art, in accompanying drawing some known features and explanation thereof may to omit be understandable.
Below in conjunction with drawings and Examples, the technical solution of the utility model is described further.
Embodiment 1
As shown in Figure 2, a kind of digital pcr analyzer, comprise control and message processing module and the light source being electrically connected with it respectively, micropore sample array and bigrid photo tft array, described micropore sample array is arranged between light source and bigrid photo tft array, described micropore sample array comprises multiple micropores sample room, described bigrid photo tft array comprises multiple bigrid photo tfts, and described micropore sample room is corresponding one by one in vertical direction with bigrid photo tft.
As shown in Figure 3-4, in specific implementation process, described light source, micropore sample array and bigrid photo tft array are arranged from top to bottom, described bigrid photo tft comprises glass substrate, dark grid, bottom dielectric layer, channel layer, source electrode, drain electrode, top dielectric, photogate and wave filtering layer, dark grid is arranged on glass substrate, bottom dielectric layer is arranged on glass substrate and covers dark grid, channel layer is arranged in bottom dielectric layer, source electrode and drain electrode are arranged on channel layer, top dielectric is arranged on semiconductor channel layer and covers source electrode and drain electrode, the photogate of transparent and electrically conductive is arranged in top dielectric, wave filtering layer is arranged in top dielectric and covers photogate, or wave filtering layer is arranged at photogate top.
As shown in Fig. 5-6, in specific implementation process, described light source, micropore sample array and bigrid photo tft array are arranged from top to bottom, described bigrid photo tft comprises glass substrate, dark grid, bottom dielectric layer, channel layer, source electrode, drain electrode, top dielectric, photogate and wave filtering layer, dark grid is arranged under glass substrate, bottom dielectric layer is arranged under glass substrate and covers dark grid, channel layer is arranged under bottom dielectric layer, source electrode and drain electrode are arranged under channel layer, top dielectric is arranged under semiconductor channel layer and covers source electrode and drain electrode, the photogate of transparent and electrically conductive is arranged under top dielectric, wave filtering layer is arranged under top dielectric and covers photogate, or wave filtering layer is arranged at photogate below.
In specific implementation process, the material of described channel layer is non-crystalline silicon, organic semiconductor, polysilicon or the oxide with optoelectronic induction function.
In specific implementation process, described light source is high-brightness LED or OLED.
In specific implementation process, be made up of transparent material described micropore sample room, and fluorescence or exciting light are had to certain transmitance.
In specific implementation process, the size of described micropore sample room is less than or equal to bigrid photo tft, to reduce optical crosstalk.
In specific implementation process, described micropore sample room is microfluidic control device.
The utility model provides a kind of digital pcr analyzer, comprise control and message processing module and the light source being electrically connected with it respectively, micropore sample array and bigrid photo tft array, described micropore sample array is arranged between light source and bigrid photo tft array, described micropore sample array comprises multiple micropores sample room, described bigrid photo tft array comprises multiple bigrid photo tfts, and described micropore sample room is corresponding one by one in vertical direction with bigrid photo tft. Sample is arranged in micropore sample room, control and message processing module control light source irradiate sample, dyestuff that be pre-mixed in sample and that combine in the large molecule of particular organisms is stimulated, send fluorescence, after filtering, responded to by the bigrid photo tft corresponding with micropore sample room and be converted into the signal of telecommunication, through controlling, processing is that available light intensity is corresponding with the specific macromolecular content of each sample with message processing module, thereby can calculate the concentration of large biological molecule. The utlity model has the advantage that volume is little, cost is low.
The corresponding same or analogous parts of same or analogous label;
In accompanying drawing, describe the term of position relationship only for exemplary illustration, can not be interpreted as the restriction to this patent;
Obviously, above-described embodiment of the present utility model is only for the utility model example is clearly described, and is not the restriction to embodiment of the present utility model. For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description. Here without also giving exhaustive to all embodiments. All any amendments of doing within spirit of the present utility model and principle, be equal to and replace and improvement etc., within all should being included in the protection domain of the utility model claim.
Claims (8)
1. a digital pcr analyzer, it is characterized in that, comprise control and message processing module and the light source being electrically connected with it respectively, micropore sample array and bigrid photo tft array, described micropore sample array is arranged between light source and bigrid photo tft array, described micropore sample array comprises multiple micropores sample room, described bigrid photo tft array comprises multiple bigrid photo tfts, and described micropore sample room is corresponding one by one in vertical direction with bigrid photo tft.
2. digital pcr analyzer according to claim 1, it is characterized in that, described light source, micropore sample array and bigrid photo tft array are arranged from top to bottom, described bigrid photo tft comprises glass substrate, dark grid, bottom dielectric layer, channel layer, source electrode, drain electrode, top dielectric, photogate and wave filtering layer, dark grid is arranged on glass substrate, bottom dielectric layer is arranged on glass substrate and covers dark grid, channel layer is arranged in bottom dielectric layer, source electrode and drain electrode are arranged on channel layer, top dielectric is arranged on semiconductor channel layer and covers source electrode and drain electrode, the photogate of transparent and electrically conductive is arranged in top dielectric, wave filtering layer is arranged in top dielectric and covers photogate, or wave filtering layer is arranged at photogate top.
3. digital pcr analyzer according to claim 1, it is characterized in that, described light source, micropore sample array and bigrid photo tft array are arranged from top to bottom, described bigrid photo tft comprises glass substrate, dark grid, bottom dielectric layer, channel layer, source electrode, drain electrode, top dielectric, photogate and wave filtering layer, dark grid is arranged under glass substrate, bottom dielectric layer is arranged under glass substrate and covers dark grid, channel layer is arranged under bottom dielectric layer, source electrode and drain electrode are arranged under channel layer, top dielectric is arranged under semiconductor channel layer and covers source electrode and drain electrode, the photogate of transparent and electrically conductive is arranged under top dielectric, wave filtering layer is arranged under top dielectric and covers photogate, or wave filtering layer is arranged at photogate below.
4. digital pcr analyzer according to claim 3, is characterized in that, the material of described channel layer is non-crystalline silicon, organic semiconductor, polysilicon or the oxide with optoelectronic induction function.
5. digital pcr analyzer according to claim 1, is characterized in that, described light source is high-brightness LED or OLED.
6. digital pcr analyzer according to claim 1, is characterized in that, be made up of the transparent material that can see through fluorescence or exciting light described micropore sample room.
7. digital pcr analyzer according to claim 1, is characterized in that, the size of described micropore sample room is less than or equal to bigrid photo tft.
8. according to the digital pcr analyzer described in claim 1-7 any one, it is characterized in that, described micropore sample room is microfluidic control device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520863323.3U CN205262967U (en) | 2015-11-02 | 2015-11-02 | Digit PCR analysis appearance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520863323.3U CN205262967U (en) | 2015-11-02 | 2015-11-02 | Digit PCR analysis appearance |
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| Publication Number | Publication Date |
|---|---|
| CN205262967U true CN205262967U (en) | 2016-05-25 |
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| CN201520863323.3U Active CN205262967U (en) | 2015-11-02 | 2015-11-02 | Digit PCR analysis appearance |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106546743A (en) * | 2016-11-04 | 2017-03-29 | 中山大学 | A kind of real-time monitoring system and monitoring method of amniotic embolism serological index |
| CN107255710A (en) * | 2017-06-01 | 2017-10-17 | 广东顺德中山大学卡内基梅隆大学国际联合研究院 | Multichannel micro-fluidic fluorescence detection device and method |
| CN113670877A (en) * | 2021-08-25 | 2021-11-19 | 华中科技大学 | Inclined top-mounted Gaussian light sheet imaging system for high-throughput digital PCR detection |
-
2015
- 2015-11-02 CN CN201520863323.3U patent/CN205262967U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106546743A (en) * | 2016-11-04 | 2017-03-29 | 中山大学 | A kind of real-time monitoring system and monitoring method of amniotic embolism serological index |
| CN107255710A (en) * | 2017-06-01 | 2017-10-17 | 广东顺德中山大学卡内基梅隆大学国际联合研究院 | Multichannel micro-fluidic fluorescence detection device and method |
| CN107255710B (en) * | 2017-06-01 | 2019-07-09 | 广东顺德中山大学卡内基梅隆大学国际联合研究院 | Multichannel micro-fluidic fluorescence detection device and method |
| CN113670877A (en) * | 2021-08-25 | 2021-11-19 | 华中科技大学 | Inclined top-mounted Gaussian light sheet imaging system for high-throughput digital PCR detection |
| CN113670877B (en) * | 2021-08-25 | 2022-05-10 | 华中科技大学 | Inclined top-mounted Gaussian light sheet imaging system for high-throughput digital PCR detection |
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| GR01 | Patent grant |