CN105754900A - Novel species of Erwinia alhagi facilitating drought resistance of crops and application of novel species of Erwinia alhagi - Google Patents

Novel species of Erwinia alhagi facilitating drought resistance of crops and application of novel species of Erwinia alhagi Download PDF

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CN105754900A
CN105754900A CN201610228010.XA CN201610228010A CN105754900A CN 105754900 A CN105754900 A CN 105754900A CN 201610228010 A CN201610228010 A CN 201610228010A CN 105754900 A CN105754900 A CN 105754900A
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erwinia
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CN105754900B (en
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张磊
陈超琼
辛凯芸
沈锡辉
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Northwest A&F University
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Abstract

The invention discloses a novel species of Erwinia alhagi facilitating drought resistance of crops and application of the novel species of Erwinia alhagi.The strain is obtained by separating tissues of a leaf segment of alhagi collected in a desert region of the Xinjiang province in China, the code of the strain is LTYR-11Z, and the strain was deposited in the China Center for Type Culture Collection on January 18, 2016 with the collection number of CCTCC M 2016052, and is classified and named as Erwinia alhag.The Erwinia alhagi LTYR-11Z has plant growth promoting effects of generating heteroauxing as plant growth hormone, secreting siderophore and dissolving hard dissolved inorganic phosphate and the like, and has a certain inhibiting effect on Bipolaris sorokiniana as plant pathogenic fungi.A living bacteriaagent prepared from the novel species of Erwinia alhagi can serve as an inoculant and is used in agricultural production of drought habitat, growth of crops is facilitated, and drought resistance of the crops is improved.

Description

One strain promotees crop siccocolous Erwinia novel species and application thereof
Technical field
The invention belongs to microbial technology field, relate to a strain and promote crop siccocolous Erwinia novel species and application thereof.
Background technology
It is known that shortage of water resources and the salinization of soil are the subject matter that whole world agricultural production faces.Along with global climate deteriorates, the extreme weather such as high temperature, arid, flood, low temperature freezing-disaster occurs all over the world again and again, also produces world food and constitutes grave danger.According to statistics, worldwide, tillable soil is less than 10%, and major part soil is in the adverse circumstances such as arid, salting, marsh, cold soil.The adverse environmental factors such as the saline and alkaline high temperature low temperature of arid are the abiotic stress factors of suppression growth and development of plants, cause a series of Morphology And Physiology Biochemical changes, even result in whole plant dead time serious.And, constantly increasing of population is increasing to the pressure of grain demand, also in the urgent need to cultivating the industrial crops adapting to various environment stresses.Therefore, how Crop Improvement resistance is always the Agro-ecology hot issue that countries in the world are paid close attention to.
In addition to the inherited character of plant itself, recent studies indicate that, present in some plant tissues, interior raw microorganism is resisted during extraneous poor environment is coerced at host plant and is played an important role.Endophyte of plant can regulate Under Drought Stress in Plant tissue active oxygen in terms of the osmotic adjustment of plant, antioxidant system, drought related gene, physical signs and hormonal balance etc. and poison and Osmotic balance, thus alleviate the drought stress injury to plant, make plant also can grow under hydropenia environment.Therefore, it can develop endophyte of plant resource and coerce a favourable instrument of pressure as alleviation drought-hit area Crops Drought.Some results of study display that, endophyte of plant gives the most separated environment of anti-adversity ability of host plant and has relation one to one.Such as, the endophyte of plant separated from coastal, arid and high temperature habitat gives the host plant resistance to salt, arid and high temperature respectively.Therefore, searching endophyte resource the plant tissue of drought stress environment is lived in for a long time from nature, can assist in crop with exploitation and resist the new microbe agent of drought stress, be applied to the agricultural production of drought and water-scarce area, be the new way of following drought-hit area crop anti-adversity improvement.
Summary of the invention
It is an object of the invention to the above-mentioned deficiency overcome existing for prior art, it is provided that a strain has Erwinia novel species and the application thereof promoting the drought-resistant function of crop, this strain can be applicable to improve the drought-resistant ability of crop.
Its technical scheme is:
One strain promotees crop siccocolous Erwinia novel species, the camel thorn leaf portion separate tissue gathered from Xinjiang of China Desert Area obtains, code name is LTYR-11Z, this bacterial strain is preserved in China typical culture collection center on January 18th, 2016, preservation address is Wuhan, China Wuhan University, preserving number is CCTCC M 2016052, and Classification And Nomenclature is camel thorn Erwinia (Erwinia alhagi).
Preferably, described Erwinia novel species form and cultural characteristic are particularly as follows: in LB culture medium after 30 DEG C of cultivation 1d, bacterium colony presents yellow semi-moist convex shape；It is shaft-like through this bacterium cell of transmission electron microscope observing, there is flagella structural.
Preferably, the 16S rRNA gene order of described Erwinia novel species is as shown in SEQ ID NO.1.
Preferably, the recA gene fragment order of described Erwinia novel species is as shown in SEQ ID NO.2.
Preferably, the gpd gene fragment order of described Erwinia novel species is as shown in SEQ ID NO.3.
Preferably, described Erwinia novel species has the effect dissolving slightly solubility Phos.
Preferably, described Erwinia novel species can produce heteroauxing (IAA).
Preferably, described Erwinia novel species can produce siderophore (siderophore).
The Erwinia novel species of the present invention application during improving crop drought resistance.
Preferably, described crop is Semen Tritici aestivi and Chinese cabbage.
The Erwinia novel species of the present invention application in promoting process of crop growth.
Beneficial effects of the present invention:
Erwinia novel species of the present invention can produce auxin heteroauxing, promotes that plant tissue growth is grown.This strain can also secrete siderophore, dissolves slightly solubility inorganic phosphate, promotes that the inorganic nutritive element such as ferrum, phosphorus is absorbed by symbiosis host plant thus promotes that it grows.
Erwinia novel species of the present invention has certain antagonism to plant pathogenic fungi Helminthosporium, is conducive to improving crop and resists pathogenic fungi disease ability.
The leaf portion separate tissue of the drought-enduring plant camel thorn that Erwinia novel species of the present invention grows from the Desert Area of Xinjiang Extreme drought obtains, and it can be grown surely in Semen Tritici aestivi and Chinese cabbage etc. crops body, and improves the host plant resistance to drought stress.
Accompanying drawing explanation
Fig. 1 is Erwinia alhagi LTYR-11Z bacterium colony photo in LB culture medium and R2A culture medium；
Fig. 2 is the bacterium colony photo that Erwinia alhagi LTYR-11Z produces levan in LB+5% sucrose medium；
Fig. 3 is the phylogenetic tree that Erwinia alhagi LTYR-11Z builds according to 16S rRNA gene order with associative mode bacterial strain；
Fig. 4 is Erwinia alhagi LTYR-11Z photo of molten phosphorus on Phos solid medium；
Fig. 5 is the photo that Erwinia alhagi LTYR-11Z produces siderophore in CAS culture medium；
Fig. 6 is the flat board face-off experiment photo of Erwinia alhagi LTYR-11Z and Helminthosporium；
Fig. 7 be do not connect the comparison of wheat seedling (the 3rd basin) growth conditions of bacterium wheat seedling (the second basin) and inoculation Erwinia alhagi LTYR-11Z under drought stress conditions and two groups compare (the first basin and the 4th basin) without arid；
Fig. 8 is not connect bacterium wheat seedling under drought stress conditions to compare with root length and the stem length of the wheat seedling of inoculation Erwinia alhagi LTYR-11Z；
Fig. 9 is not connect bacterium wheat seedling under drought stress conditions to compare with the fresh weight of the wheat seedling of inoculation Erwinia alhagi LTYR-11Z；
Figure 10 is the comparison not connecing bacterium Plantula Brassicae chinensis seedling (the first basin) under drought stress conditions with Plantula Brassicae chinensis seedling (the second basin) growth conditions of inoculation Erwinia alhagi LTYR-11Z；
Figure 11 is not connect bacterium Plantula Brassicae chinensis seedling under drought stress conditions to compare with the fresh weight of the Plantula Brassicae chinensis seedling of inoculation Erwinia alhagi LTYR-11Z.
Detailed description of the invention
With specific embodiment, the method for the present invention is described in more detail below in conjunction with the accompanying drawings.
The Erwinia novel species that the present invention relates to, the camel thorn leaf portion separate tissue gathered from Xinjiang of China Desert Area obtains, code name is LTYR-11Z, this bacterial strain is preserved in China typical culture collection center on January 18th, 2016, preserving number is CCTCC M 2016052, and Classification And Nomenclature is camel thorn Erwinia (Erwinia alhagi).
The separation of Erwinia novel species the most of the present invention
Camel thorn plants sample is gathered from desert region in Xinjiang, its leaf portion plant tissue is carried out surface sterilization, it is cut into fragment with the shears of sterilization to be positioned on the R2A solid medium containing 50 μ g/mL cycloheximides, cultivate 1 week for 28 DEG C, the rice white lawn grown around picking blade, line obtains bacterial strain LTYR-11Z after purification, taxonomic identification is carried out through phenotype and inherited characteristic, determine that it is the novel species of Erwinia (Erwinia), named camel thorn Erwinia (Erwinia alhagi)
The morphological characteristic of Erwinia novel species the most of the present invention
As it is shown in figure 1, after camel thorn Erwinia (Erwinia alhagi LTYR-11Z) streak inoculation is cultivated 24h on LB flat board, formed circular, diameter 2-5mm, opaque, semi-moist, protruding yellow color colonies (left)；After this bacterial strain streak inoculation is cultivated 24h on R2A flat board, formed circular, diameter 2-4mm, opaque, semi-moist, protruding rice white bacterium colony (right).Transmission electron microscope microscopic findings shows that the cell of this bacterial strain is shaft-like, has flagellum, it is possible to motion.
The physio-biochemical characteristics of Erwinia novel species the most of the present invention
Erwinia novel species Erwinia alhagi LTYR-11Z can grow in the many kinds of solids culture medium such as TSA, R2A, Marine Agar 2216, Nutrient Agar, MacConkey Agar, LB, it is resistant to 0-7%NaCl, growth temperature range 7-48 DEG C, growth pH scope is 5.0-9.0；Belong to facultative anaerobe, at the equal well-grown of aerobic and oxygen free condition；Produce catalase and cytochrome oxidase, may utilize the various saccharides such as glucose, arabinose, mannose, maltose and grow as sole carbon source, it is possible to caseinhydrolysate.As in figure 2 it is shown, by this strain streak inoculation on the LB flat board containing 5% sucrose, form thick white, dome-type macrocolony, show that sucrose degradation can be changed into levan by this bacterial strain.
4.16S rRNA gene, recA gene and gpd gene sequencing
nullExtract the genomic DNA of bacterial strain LTYR-11Z，16S rRNA gene universal primer 27f (5 '-AGAGTTTGATCCTGGCTCAG-3 ') and 1492r (5 '-CGGTTACCTTGTTACGACTT-3 ') is utilized to expand 16S rRNA genetic fragment，RecA gene universal primer RECA1 (5 '-GGTAAAGGGTCTATCATGCG-3 ') and RECA2c (5 '-CCTTCACCATACATAATTTGGA-3 ') is utilized to expand recA genetic fragment，Gpd gene universal primer GAP11 (5 '-ACGGCACTGTAGAAGTC-3 ') and GAP12c (5 '-CCAGTCTTTGTGAGACG-3 ') is utilized to expand gpd genetic fragment，It is connected into pMD19-T carrier respectively，Proceed to competent escherichia coli cell，Select positive colony to check order，Obtain 16S rRNA gene respectively、RecA gene and gpd gene order.
The 16S rRNA gene order of Erwinia novel species Erwinia alhagi LTYR-11Z is as shown in SEQ ID NO.1.This 16S rRNA gene order submission GenBank data base is carried out Multiple sequence alignments, carries out sequence similarity analysis with the type strain of effective publication, find the type strain Erwinia uzenensis YPPS 951 of itself and ErwiniaT, Erwinia piriflorinigrans CFBP 5888TWith Erwinia pyrifoliae DSM 12163TSequence similarity the highest, respectively 97.5%, 97.4% and 97.4%.According to the theory of famous division bacteria scholar Stackebrandt etc., the kind that the 16S rRNA gene order similarity antibacterial less than 98.7% is the most different.The 16S rRNA sequence similarity of bacterial strain LTYR-11Z type strain existing with Erwinia, below 97.5%, shows that it has larger difference with the known species of Erwinia, should be a novel species of Erwinia.The 16S rRNA gene order of bacterial strain LTYR-11Z with Erwinia associative mode bacterial strain is usedClustalX 1.81 Software comparison,Then using adjacent method constructing system cladogram, result is as shown in Figure 3.Can be seen that from phylogenetic tree (Fig. 3), in the classification bunch of Erwinia all type strains composition, bacterial strain LTYR-11Z is positioned in an independent branch, shows that this bacterial strain has bigger difference with the type strain of other kind of Erwinia on classification position.
The part recA gene fragment order of Erwinia novel species Erwinia alhagi LTYR-11Z is as shown in SEQ ID NO.2.The type strain Erwinia uzenensis YPPS 951 that this recA gene order is nearest with its sibshipT, Erwinia piriflorinigrans CFBP 5888TWith Erwinia pyrifoliae DSM 12163TSimilarity be only 84.9%, 85.1% and 85.1%.
The part gpd gene fragment order of Erwinia novel species Erwinia alhagi LTYR-11Z is as shown in SEQ ID NO.3.This gpd gene order and type strain Erwinia uzenensis YPPS 951TWith Erwinia piriflorinigrans CFBP 5888TSimilarity be only 81.1% and 82.2%.
In sum, the sequence similarity of 16S rRNA gene order and two conservative genes of recA and gpd compares and Phylogenetic Analysis result all shows that bacterial strain LTYR-11Z has bigger diversity with the associative mode bacterial strain of Erwinia in heredity, should be a novel species of Erwinia, by its named camel thorn Erwinia (Erwinia alhagi).
Erwinia novel species the most of the present invention produces heteroauxing (IAA) ability and measures
Erwinia novel species Erwinia alhagi LTYR-11Z is inoculated in SMS fluid medium [sucrose the 10g, (NH containing 0.5mg/ml L-Trp4)2SO4 1g、K2HPO4 2g、MgSO4 0.5g、Yeast extract 0.5g、CaCO30.5g, NaCl 30g, 1000mL distilled water, pH value 7.2], 30 DEG C, 3d cultivated by 180rpm shaking table.OD with spectrophotometry bacteria suspension600Value, is then centrifuged 10min by bacteria suspension 10000rpm, takes supernatant and adds equal-volume Salkowski color solution (50mL 35%HClO4+1mL 0.5M FeCl3), lucifuge stands 30min, measures its OD530Value.Calculate bacteria concentration OD600When value is 1, the content (being calculated the content of IAA by the standard curve of IAA gradient dilution) of IAA in unit volume fermentation liquid.By measuring, the IAA yield of this Erwinia novel species is up to 17.73 μ g/ (mL OD600)。
Erwinia novel species dissolved metals the most of the present invention is tested
Erwinia novel species Erwinia alhagi LTYR-11Z is inoculated in slightly solubility Phos solid medium [10g glucose, 5.0g Ca3(PO4)2、0.5g(NH4)2SO4、0.2g NaCl、0.2g KCl、0.03g MgSO4·7H2O、0.03g MnSO4、0.003g FeSO4, 0.5g yeast extract, 20g agar, 1000mL distilled water, pH value 6.8～7.0], be placed in 30 DEG C of cultivations, seen whether that degraded is irised out existing.As shown in Figure 4, after Phos solid medium cultivates 3d, there is transparent circle to occur around the lawn of this Erwinia novel species, show that this strain can dissolve slightly solubility Phos.
Erwinia novel species the most of the present invention produces the detection of siderophore (siderophore) ability
Take 60.5mg CAS (chromazurine) and be dissolved in 50mL ddH2In O, with 10mL ferrous solution (1mmol/L FeCl3With 10mmol/L HCl) solution mix homogeneously, obtain solution A；Take 72.9mg HDTMA (cetyl trimethylammonium bromide) and be dissolved in 40mL ddH2O, obtains solution B；Solution A is slowly added in B solution, fully mixes, obtain CAS dye liquor.Prepared by CAS detection flat board: every 100mL is containing 20% sucrose solution 1mL, 10% acid hydrolysis casein 3mL, 1mmol/L CaCl2100 μ L, 1mmol/L MgSO42mL, agar 1.8g, be slowly added to 10 × MM9 saline solution (Na when about 60 DEG C2HPO4·12H2O 2.427g, N aH2PO4·2H2O 0.5905g, KH2PO40.075g, NH4Cl 0.250g, NaCl0.125g, 100mL ddH2O, regulates pH to 6.8) and each 5mL of CAS dye liquor, fully shake up (but not producing bubble), obtain blue detection culture medium.Erwinia novel species Erwinia alhagi LTYR-11Z is inoculated in detection culture medium central, is placed in 30 DEG C of cultivations, seen whether that variable color is irised out existing.As it is shown in figure 5, after cultivating 3d in CAS detection culture medium, obvious yellow halo occurs around the lawn of this Erwinia novel species, shows that it can produce siderophore.
The Erwinia novel species the most of the present invention inhibitory action to Helminthosporium
Plant pathogenic fungi Helminthosporium is inoculated in PDA culture medium (Rhizoma Solani tuber osi 200g, glucose 20g, agar 15～20g, tap water 1000mL), cultivate 2-3d for 30 DEG C, beat on above-mentioned culture plate with card punch and take 1 bacterium cake, then fresh 1/4PDA culture plate centre it is forwarded to, the Erwinia novel species Erwinia alhagi LTYR-11Z activated is connected to same 1/4PDA culture plate edge simultaneously, 30 DEG C cultivate 2-3d after observe the bacterial strain LTYR-11Z antagonistic effect to Helminthosporium.As shown in Figure 6, this Erwinia novel species has certain inhibitory action to plant pathogenic fungi Helminthosporium.
9. inoculate Erwinia novel species of the present invention and improve Semen Tritici aestivi and the Chinese cabbage seedling resistance to drought stress
Single bacterium colony on Erwinia novel species Erwinia alhagi LTYR-11Z flat board is accessed in 5mL TSB fluid medium, 30 DEG C, after 200rpm shakes bacterium 12h, transfer in the triangular flask containing 200mL TSB liquid medium, in 30 DEG C, on 200rpm shaking table concussion cultivate, reaching thalline exponential phase after 24h, 6000rpm is centrifugal collects thalline.After washing thalline twice with the tap water of sterilizing, it is diluted to 108～109CFU/mL is as bacteria agent.
Vermiculitum and soil 1:3 by volume are mixed to obtain planting matrix, loads in strain bag, every bag of 450g, seal rear 121 DEG C of sterilizing 25min, then the mixed soil after sterilizing is loaded in plastic flowerpot.
Select full seed, the seed of Shanxi of the same size No. 47 Semen Tritici aestivis of wheat and four seasons Huang seedling Plantula Brassicae chinensis seed, first by 75% soak with ethanol 30s, outwell liquid, add 0.1%HgCl2Soak wheat seed 7min (Plantula Brassicae chinensis seed 3min), then with aseptic washing 5～6 times.Being put into by wheat seed in the moistening double-layer filter paper of sterilizing, be placed in 25 DEG C, dark accelerating germination 2d, growing way is identical, full shoots of wheat is transplanted in the soil of sterilizing, every basin kind 3, waters the tap water 40mL of sterilizing；And directly seed is planted in the mixed soil of sterilizing after the sterilization of Plantula Brassicae chinensis the surface of the seed, every basin kind one, three basins repeat, and water the tap water 20mL of sterilizing.
It is placed in potted plant for ready Semen Tritici aestivi in 25 DEG C of greenhouses, illumination every day 14h, every other day water the tap water 30mL of sterilizing, after the week, by ready Erwinia novel species Erwinia alhagi LTYR-11Z active bacteria formulation 40mL (by every gram of soil 107～108Number of viable is inoculated) it is poured onto the root position of Semen Tritici aestivi, arrange simultaneously and do not meet the tap water 40mL after the matched group of bacterium waters sterilizing, each process 3 basin repeats.About one week, by the time Semen Tritici aestivi grew to for two leaf one heart stages, docking bacterium process group and do not connect bacterium matched group and carry out Osmotic treatment simultaneously, stop watering 12 days, after recovering to irrigate 2 days, record the health status of wheat seedling and take a picture, then gathering in the crops plant, measure and add up the growth indexes such as the average root length of each process, plant height, fresh weight, dry weight.It is illustrated in figure 7 four groups of process of Semen Tritici aestivi potted plant experiment, first basin is not connect bacterium also not carry out the wheat seedling of drought stress process from left to right, second basin is not connect bacterium but the wheat seedling of drought stress process, 3rd basin be inoculation Erwinia alhagi LTYR-11Z and drought stress process wheat seedling, the 4th basin be inoculation Erwinia alhagi LTYR-11Z but without drought stress process wheat seedling.As can be seen from Figure 7, the wheat seedling (the 3rd basin) being vaccinated with this Erwinia novel species under the conditions of equal Osmotic treatment keeps bud green, normal growth, and the wheat seedling (the second basin) not connecing bacterium is vaccinated with the growth promoter of wheat seedling of this Erwinia novel species under the conditions of there is withered, short and small phenomenon, i.e. Osmotic treatment and health status is substantially better than the comparison not connecing bacterium.In Fig. 8 and Fig. 9, CK and LTYR-11Z represents wheat seedling Osmotic treatment and the wheat seedling Osmotic treatment of inoculation Erwinia novel species Erwinia alhagi LTYR-11Z not connecing bacterium respectively.As shown in Figure 8, the wheat seedling being vaccinated with this Erwinia novel species during Osmotic treatment averagely increases by 16.2% with the comparison phase specific root length not connecing bacterium, and stem length averagely increases by 20.2%.As it is shown in figure 9, the wheat seedling fresh weight compared with the comparison not connecing bacterium being vaccinated with this Erwinia novel species during Osmotic treatment increases by 86.1%.Fig. 7, the result of 8 and 9 shows under drought stress conditions, and the Biomass of the wheat seedling that application of Erwinia novel species Erwinia alhagi LTYR-11Z dramatically increases, and significantly improves the resistivity of drought stress.
It is placed in potted plant for ready Plantula Brassicae chinensis in 25 DEG C of greenhouses, illumination every day 14h, every other day water the tap water 20mL of sterilizing, after three weeks, by ready Erwinia novel species Erwinia alhagi LTYR-11Z active bacteria formulation 20mL (by every gram of soil 107～108Number of viable is inoculated) it is poured onto the root position of Plantula Brassicae chinensis, arrange simultaneously and do not connect the matched group of bacterium and water the tap water 20mL of sterilizing, each process 3 basin repeats.After about three weeks, Plantula Brassicae chinensis is connect bacterium process group and does not connect bacterium matched group and carry out Osmotic treatment simultaneously, stop watering 11 days, after recovering to irrigate 2 days, record the health status of Plantula Brassicae chinensis seedling and take a picture, then results seedling Herb, measures and adds up the growth indexes such as the average root length of each process, plant height, fresh weight, dry weight.Being two groups of Osmotic treatment of Plantula Brassicae chinensis potted plant experiment as shown in Figure 10, the first basin is the Plantula Brassicae chinensis seedling Osmotic treatment not connecing bacterium from left to right, and the second basin is the Plantula Brassicae chinensis seedling Osmotic treatment of inoculation Erwinia novel species Erwinia alhagi LTYR-11Z.As can be seen from Figure 10, the Plantula Brassicae chinensis seedling (the second basin) being vaccinated with this Erwinia novel species under the conditions of equal Osmotic treatment keeps bud green, normal growth, and the Plantula Brassicae chinensis seedling (the first basin) not connecing bacterium is vaccinated with the growth promoter of Plantula Brassicae chinensis seedling of this Erwinia novel species under the conditions of there is withered, short and small phenomenon, i.e. Osmotic treatment and health status is substantially better than the comparison not connecing bacterium.In Figure 11, CK and LTYR-11Z represents Plantula Brassicae chinensis seedling Osmotic treatment and the Plantula Brassicae chinensis seedling Osmotic treatment of inoculation Erwinia novel species Erwinia alhagi LTYR-11Z not connecing bacterium respectively.As shown in figure 11, it is applied with the Plantula Brassicae chinensis seedling of this Erwinia novel species active bacteria formulation fresh weight after drought stress processes to compare and do not connect the comparison of bacterium and add 86.8%.
The above; it is only the present invention preferably detailed description of the invention; protection scope of the present invention is not limited to this; any those familiar with the art is in the technical scope of present disclosure, and the simple change of the technical scheme that can become apparent to or equivalence are replaced and each fallen within protection scope of the present invention.

Claims (11)

1. a strain promotees crop siccocolous Erwinia novel species, it is characterised in that: this strain belongs to Erwinia, and code name is LTYR-11Z, this bacterial strain is preserved in China typical culture collection center on January 18th, 2016, and preserving number is CCTCC M 2016052, Classification And Nomenclature is camel thorn Erwinia Erwinia alhagi.
Promote crop siccocolous Erwinia novel species the most according to claim 1, it is characterised in that its form and cultural characteristic Particularly as follows: in LB culture medium after 30 DEG C of cultivation 1d, bacterium colony presents yellow semi-moist convex shape；Should through transmission electron microscope observing Strain cell is shaft-like, has flagella structural.
Promote crop siccocolous Erwinia novel species the most according to claim 1, it is characterised in that the 16S of described strain RRNA gene order is as shown in SEQ ID NO.1.
Promote crop siccocolous Erwinia novel species the most according to claim 1, it is characterised in that the recA base of described strain Because sequence is as shown in SEQ ID NO.2.
Promote crop siccocolous Erwinia novel species the most according to claim 1, it is characterised in that the gpd base of described strain Because sequence is as shown in SEQ ID NO.3.
Promote crop siccocolous Erwinia novel species the most according to claim 1, it is characterised in that described strain has dissolving The effect of slightly solubility Phos.
Promote crop siccocolous Erwinia novel species the most according to claim 1, it is characterised in that described strain can produce Heteroauxing.
Promote crop siccocolous Erwinia novel species the most according to claim 1, it is characterised in that described strain can produce Siderophore.
9. the application during improving crop drought resistance of the Erwinia novel species described in claim 1.
The Erwinia novel species the most according to claim 9 application during improving crop drought resistance, it is characterised in that Described crop is Semen Tritici aestivi and Chinese cabbage.
The application in promoting process of crop growth of the Erwinia novel species described in 11. claim 1.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109182566A (en) * 2018-09-21 2019-01-11 广东省农业科学院植物保护研究所 A method of prevention and treatment banana dasheen bacterial soft rot
CN111808777A (en) * 2020-07-28 2020-10-23 南京工业大学 Pantoea camelina and application thereof
CN112940992A (en) * 2021-04-23 2021-06-11 安徽农业大学 Tea tree endophytic bacteria with plant growth promoting effect, microbial inoculum and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104694422A (en) * 2015-02-11 2015-06-10 齐齐哈尔大学 Separated pantoea ananatis HYL01 strain and application thereof in promotion of root growth of watermelons
CN105219651A (en) * 2015-08-17 2016-01-06 三峡大学 A kind ofly improve Rice Resistance arid, interior raw Aspergillus fumigatus that salt damage coerces ability and application thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104694422A (en) * 2015-02-11 2015-06-10 齐齐哈尔大学 Separated pantoea ananatis HYL01 strain and application thereof in promotion of root growth of watermelons
CN105219651A (en) * 2015-08-17 2016-01-06 三峡大学 A kind ofly improve Rice Resistance arid, interior raw Aspergillus fumigatus that salt damage coerces ability and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
AKBAR A ET AL: "Sphingomonas hylomeconis sp.nov.,isolated from the stem of Hylomecon japonica", 《INT J SYST EVOL MICROBIOL》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109182566A (en) * 2018-09-21 2019-01-11 广东省农业科学院植物保护研究所 A method of prevention and treatment banana dasheen bacterial soft rot
CN109182566B (en) * 2018-09-21 2021-07-20 广东省农业科学院植物保护研究所 Method for preventing and treating bacterial soft rot of canna edulis ker
CN111808777A (en) * 2020-07-28 2020-10-23 南京工业大学 Pantoea camelina and application thereof
CN111808777B (en) * 2020-07-28 2022-02-11 南京工业大学 Pantoea camelina and application thereof
CN112940992A (en) * 2021-04-23 2021-06-11 安徽农业大学 Tea tree endophytic bacteria with plant growth promoting effect, microbial inoculum and application thereof
CN112940992B (en) * 2021-04-23 2022-07-08 安徽农业大学 Tea tree endophytic bacteria with plant growth promoting effect, microbial inoculum and application thereof

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