CN105752955A - Method for dissolving mid-low-grade phosphate ore by utilizing activated sludge microbial flora - Google Patents
Method for dissolving mid-low-grade phosphate ore by utilizing activated sludge microbial flora Download PDFInfo
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Abstract
The invention relates to a method for dissolving mid-low-grade phosphate ore by utilizing activated sludge microbial flora. The method comprises the following steps: 1) performing enrichment culture on the activated sludge microbial flora, namely, performing the enrichment culture on the activated sludge microbial flora to obtain an activated sludge microbial flora enrichment culture solution; 2) performing hardening culture on the activated sludge microbial flora, namely, performing the hardening culture on the activated sludge microbial flora enrichment culture solution to obtain an activated sludge microbial flora hardening culture solution; 3) dissolving the mid-low-grade phosphate ore by the activated sludge microbial flora hardening culture solution, namely, adding a non-phosphorus culture medium and the mid-low-grade phosphate ore into a container, and then adding the activated sludge microbial flora hardening culture solution for culturing. The method provided by the invention is simple in process, low in culture condition requirements and low in production cost; the phosphate dissolving rate of the mid-low-grade phosphate ore reaches over 60 percent. By adopting the method, activated sludge can be changed from waste into treasure, and a positive effect on the utilization and treatment of municipal domestic wastewater is achieved.
Description
Technical field
The present invention relates to phosphorus ore exploitation processing technique field, particularly relate to a kind of method utilizing active sludge microorganism flora to dissolve mid low grade phosphate rock.
Background technology
Phosphorus ore is the raw material that industrial production phosphate fertilizer is indispensable, and according to statistics, the phosphorus ore of about more than 80% contributes to the production of phosphate fertilizer in the world.At present, the mode of industrial production phosphate fertilizer has a lot, mainly has wet method and full-boiled process two kinds, but these methods are generally only suitable for processing high-grade phosphorus ore.
China's phosphate rock resource enriches, but is mostly mid low grade phosphate rock, and in Ore, phosphorus pentoxide content is low, and disseminated grain size is little, and mineral composition is complicated.If adopt conventional wet or full-boiled process process these Mid-and low-grade phosphate rock resources and are processed into phosphate fertilizer, then can cause that production cost is higher, low in economic efficiency, and comparatively serious environmental pollution can be brought.
Microbial technique is widely used at mineral manufacture field.In phosphorus ore containing, mainly utilize the phosphorus in biosolubilization phosphorus ore and the phosphorus in the elimination mineral such as high-phosphorus iron ore, high-phosphorus manganese.Contrast tradition beneficiation method, microorganism ore dressing has that technique is simple, environmental friendliness and low power consumption and other advantages.Current chemical phosphatic ferfilizer production cost is high, expensive, if able to utilize the Mid-and low-grade phosphate rock resources of the microbial treatments China's abundant of abundance in nature and make the biologic phosphorus fertilizer of relative low price, from application prospect, this all has far-reaching strategic importance for the scientific utilization of China's Mid-and low-grade phosphate rock resources, minimizing environmental pollution and development Phosphate Fertilizer Industry etc..
Current research report has been found that multiple-microorganism such as antibacterial, fungus and actinomycetes etc. all have the ability dissolving mid low grade phosphate rock.But, for research single or principal component the several strains of many employings of biosolubilization mid low grade phosphate rock, the research dissolving mid low grade phosphate rock with microorganism species natural, non-principal component is still rare.Active sludge microorganism flora is mostly used for the dephosphorization research of sanitary sewage, has no the molten phosphorus research being applied to mid low grade phosphate rock at present.
Summary of the invention
It is an object of the invention to provide a kind of method utilizing active sludge microorganism flora to dissolve mid low grade phosphate rock, the method has the advantages that molten phosphorus rate is high, technique is simple, condition of culture requirement is low, production cost is low.
To achieve these goals, technical scheme is as follows:
A kind of method utilizing active sludge microorganism flora to dissolve mid low grade phosphate rock, comprise the steps: 1) enrichment culture of active sludge microorganism flora: the activated sludge containing microorganism species is carried out enrichment culture, obtains active sludge microorganism enriching floras culture fluid；2) domestication of active sludge microorganism flora is cultivated: active sludge microorganism enriching floras culture fluid carries out domestication and cultivates, and obtains active sludge microorganism flora domestication culture fluid；3) active sludge microorganism flora domestication culture fluid dissolves mid low grade phosphate rock: without phosphorus culture medium and mid low grade phosphate rock are added in container, adds active sludge microorganism flora domestication culture fluid and cultivates.
Step 1) enrichment culture of described active sludge microorganism flora includes a that phosphorus culture medium and activated sludge 1:0.3-0.5 by volume will be had to load in container, is placed in constant-temperature table by container shaken cultivation, obtains active sludge microorganism enriching floras culture fluid A；B will have phosphorus culture medium and active sludge microorganism enriching floras culture fluid A 1:0.3-0.5 by volume to load in container, be placed in constant-temperature table by container shaken cultivation, the active sludge microorganism enriching floras culture fluid A being again enriched with；C repeats step b1-3 time, obtains active sludge microorganism enriching floras culture fluid.
Described constant-temperature table temperature is 22-30 DEG C, and rotating speed is 100-160 rev/min, and the shaken cultivation time is 3-5 days.
Step 2) domestication of described active sludge microorganism enriching floras culture fluid cultivates and includes 1. loading in container by without phosphorus culture medium and active sludge microorganism enriching floras culture fluid 1:0.2-0.3 by volume, 5-10g mid low grade phosphate rock is added again by the without phosphorus culture medium of every 1000ml, it is placed in constant-temperature table by container after mix homogeneously shaken cultivation, obtains active sludge microorganism flora domestication culture fluid B；2. without phosphorus culture medium and active sludge microorganism flora are tamed culture fluid B 1:0.2-0.3 by volume and load in container, 10-15g mid low grade phosphate rock is added again by the without phosphorus culture medium of every 1000ml, it is placed in constant-temperature table by container after mix homogeneously shaken cultivation, the active sludge microorganism flora again tamed domestication culture fluid B；3. repeating step and 2. obtain active sludge microorganism flora domestication culture fluid for 1-3 time, gradually improve the content of mid low grade phosphate rock in container during domestication, last domestication is cultivated the without phosphorus culture medium of every 1000ml and is needed mid low grade phosphate rock 15-20g.
Described constant-temperature table temperature is 22-30 DEG C, and rotating speed is 100-140 rev/min, and the shaken cultivation time is 3-5 days.
Step 3) described active sludge microorganism flora domestication culture fluid dissolves mid low grade phosphate rock and includes that without phosphorus culture medium and active sludge microorganism flora are tamed culture fluid 1:0.2-0.3 by volume and load in container, 10-20g mid low grade phosphate rock is added again by the without phosphorus culture medium of every 1000ml, container is placed in 22-30 DEG C of constant-temperature table after mix homogeneously, under 120-160 rev/min of rotating speed shaken cultivation 7-10 days.
Described activated sludge is from city domestic sewage processing plant.
Described without phosphorus culture medium consists of 10g/L glucose, 0.5g/L yeast extract, 0.5g/L (NH4)2SO4、0.2g/LKCl、0.1g/LMgSO4·7H2O、0.0001g/LMnSO4·H2O、0.0001g/LFeSO4·7H2O, solvent is sterilized water.
Described have phosphorus culture medium to consist of 10g/L glucose, 0.5g/L yeast extract, 0.5g/L (NH4)2SO4、0.2g/LKCl、0.1g/LMgSO4·7H2O、0.0001g/LMnSO4·H2O、0.0001g/LFeSO4·7H2The calcium phosphate of O, 10-20g/L, solvent is sterilized water.
In described mid low grade phosphate rock, the mass content of phosphorus pentoxide is lower than 30%, and it is 50-200 order that described mid low grade phosphate rock needs to be crushed to particle diameter before using.
The invention has the beneficial effects as follows:
1, activated sludge is from city domestic sewage processing plant, adopts the inventive method to be turned waste into wealth by activated sludge, and utilization and process to urban domestic wastewater have also functioned to positive effect.
2, the molten phosphorus rate of mid low grade phosphate rock can be reached more than 60% by the inventive method, and the scientific utilization for the Mid-and low-grade phosphate rock resources of China's abundant provides a practicable method.
3, present invention process is simple, condition of culture requires low and environmental friendliness.
Detailed description of the invention
In order to be more fully understood that the present invention, it is further elucidated with present disclosure below in conjunction with embodiment, but present disclosure is not limited solely to the following examples.
The without phosphorus nutrient media components that the present invention and embodiment adopt is: 10g glucose, 0.5g yeast extract, 0.5g (NH4)2SO4, 0.2gKCl, 0.1gMgSO4·7H2O, 0.0001gMnSO4·H2O, 0.0001gFeSO4·7H2O and 1000mL sterilized water.It is described that to have phosphorus culture medium be that to add the calcium phosphate of 10-20g/L in aforementioned without phosphorus culture medium formulated.
Embodiment 1
A kind of method utilizing active sludge microorganism flora to dissolve mid low grade phosphate rock, comprises the steps:
1) enrichment culture of active sludge microorganism flora: phosphorus culture medium and activated sludge 1:0.3 by volume will be had to load in container (such as triangular flask), container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 4 days, obtains the active sludge microorganism enriching floras culture fluid A of the 1st enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.3 by volume having phosphorus culture medium and the 1st enrichment culture is loaded in container, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 4 days, obtains the active sludge microorganism enriching floras culture fluid A of the 2nd enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.3 by volume having phosphorus culture medium and the 2nd enrichment culture is loaded in container, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 4 days, obtains the active sludge microorganism enriching floras culture fluid A of the 3rd enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.3 by volume having phosphorus culture medium and the 3rd enrichment culture is loaded in container, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 4 days, obtains the active sludge microorganism enriching floras culture fluid A of the 4th enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.3 by volume having phosphorus culture medium and the 4th enrichment culture is loaded in container, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 4 days, obtains active sludge microorganism enriching floras culture fluid (i.e. inoculation enrichment culture 5 times so repeatedly).
2) domestication of active sludge microorganism flora is cultivated: load in container (such as triangular flask) by without phosphorus culture medium and active sludge microorganism enriching floras culture fluid 1:0.25 by volume, 8g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 120 revs/min shaken cultivation 3 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 1st domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.25 by volume without phosphorus culture medium and the 1st domestication cultivated loads in container, 12g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 30 DEG C of constant-temperature tables under the rotating speed of 120 revs/min shaken cultivation 5 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 2nd domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.25 by volume without phosphorus culture medium and the 2nd domestication cultivated loads in container, 18g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 30 DEG C of constant-temperature tables under the rotating speed of 120 revs/min shaken cultivation 5 days after mix homogeneously, obtains active sludge microorganism flora domestication culture fluid (i.e. inoculation domestication cultivation 3 times so repeatedly).
3) active sludge microorganism flora domestication culture fluid dissolves mid low grade phosphate rock: add in container by without phosphorus culture medium and mid low grade phosphate rock, add active sludge microorganism flora domestication culture fluid mix homogeneously, being placed in by container afterwards in 25 DEG C of constant-temperature tables, under 160 revs/min of rotating speeds, shaken cultivation obtains titanium pigment in 10 days.Wherein the volume ratio of without phosphorus culture medium and active sludge microorganism flora domestication culture fluid is 1:0.25, and the without phosphorus culture medium of every 1000ml adds 15g mid low grade phosphate rock.
In the mid low grade phosphate rock that the present embodiment adopts, the mass content of phosphorus pentoxide is 22.4%, and molten phosphorus rate reaches 77.3%.
Embodiment 2
A kind of method utilizing active sludge microorganism flora to dissolve mid low grade phosphate rock, comprises the steps:
1) enrichment culture of active sludge microorganism flora: phosphorus culture medium and activated sludge 1:0.4 by volume will be had to load in container (such as triangular flask), container is placed in 22 DEG C of constant-temperature tables under the rotating speed of 160 revs/min shaken cultivation 3 days, obtains the active sludge microorganism enriching floras culture fluid A of the 1st enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.5 by volume having phosphorus culture medium and the 1st enrichment culture is loaded in container, container is placed in 30 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 4 days, obtains the active sludge microorganism enriching floras culture fluid A of the 2nd enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.3 by volume having phosphorus culture medium and the 2nd enrichment culture is loaded in container, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 100 revs/min shaken cultivation 5 days, obtains active sludge microorganism enriching floras culture fluid (i.e. inoculation enrichment culture 3 times so repeatedly).
2) domestication of active sludge microorganism flora is cultivated: load in container (such as triangular flask) by without phosphorus culture medium and active sludge microorganism enriching floras culture fluid 1:0.3 by volume, 5g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 30 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 4 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 1st domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.25 by volume without phosphorus culture medium and the 1st domestication cultivated loads in container, 10g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 28 DEG C of constant-temperature tables under the rotating speed of 120 revs/min shaken cultivation 3 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 2nd domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.2 by volume without phosphorus culture medium and the 2nd domestication cultivated loads in container, 15g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 30 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 5 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 3rd domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.25 by volume without phosphorus culture medium and the 3rd domestication cultivated loads in container, 20g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 22 DEG C of constant-temperature tables under the rotating speed of 100 revs/min shaken cultivation 4 days after mix homogeneously, obtains active sludge microorganism flora domestication culture fluid (i.e. inoculation domestication cultivation 4 times so repeatedly).
3) active sludge microorganism flora domestication culture fluid dissolves mid low grade phosphate rock: add in container by without phosphorus culture medium and mid low grade phosphate rock, add active sludge microorganism flora domestication culture fluid mix homogeneously, being placed in by container afterwards in 28 DEG C of constant-temperature tables, under 120 revs/min of rotating speeds, shaken cultivation obtains titanium pigment in 7 days.Wherein the volume ratio of without phosphorus culture medium and active sludge microorganism flora domestication culture fluid is 1:0.2, and the without phosphorus culture medium of every 1000ml adds 20g mid low grade phosphate rock.
In the mid low grade phosphate rock that the present embodiment adopts, the mass content of phosphorus pentoxide is 21.5%, and molten phosphorus rate reaches 63.9%.
Embodiment 3
A kind of method utilizing active sludge microorganism flora to dissolve mid low grade phosphate rock, comprises the steps:
1) enrichment culture of active sludge microorganism flora: phosphorus culture medium and activated sludge 1:0.45 by volume will be had to load in container (such as triangular flask), container is placed in 24 DEG C of constant-temperature tables under the rotating speed of 100 revs/min shaken cultivation 4 days, obtains the active sludge microorganism enriching floras culture fluid A of the 1st enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.5 by volume having phosphorus culture medium and the 1st enrichment culture is loaded in container, container is placed in 30 DEG C of constant-temperature tables under the rotating speed of 120 revs/min shaken cultivation 4 days, obtains the active sludge microorganism enriching floras culture fluid A of the 2nd enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.35 by volume having phosphorus culture medium and the 2nd enrichment culture is loaded in container, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 160 revs/min shaken cultivation 4 days, obtains the active sludge microorganism enriching floras culture fluid A of the 3rd enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.5 by volume having phosphorus culture medium and the 3rd enrichment culture is loaded in container, container is placed in 30 DEG C of constant-temperature tables under the rotating speed of 120 revs/min shaken cultivation 4 days, obtains active sludge microorganism enriching floras culture fluid (i.e. inoculation enrichment culture 4 times so repeatedly).
2) domestication of active sludge microorganism flora is cultivated: load in container (such as triangular flask) by without phosphorus culture medium and active sludge microorganism enriching floras culture fluid 1:0.3 by volume, 10g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 24 DEG C of constant-temperature tables under the rotating speed of 120 revs/min shaken cultivation 3 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 1st domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.2 by volume without phosphorus culture medium and the 1st domestication cultivated loads in container, 14g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 28 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 3 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 2nd domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.25 by volume without phosphorus culture medium and the 2nd domestication cultivated loads in container, 17g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 30 DEG C of constant-temperature tables under the rotating speed of 100 revs/min shaken cultivation 5 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 3rd domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.28 by volume without phosphorus culture medium and the 3rd domestication cultivated loads in container, 20g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 130 revs/min shaken cultivation 4 days after mix homogeneously, obtains active sludge microorganism flora domestication culture fluid (i.e. inoculation domestication cultivation 4 times so repeatedly).
3) active sludge microorganism flora domestication culture fluid dissolves mid low grade phosphate rock: add in container by without phosphorus culture medium and mid low grade phosphate rock, add active sludge microorganism flora domestication culture fluid mix homogeneously, being placed in by container afterwards in 22 DEG C of constant-temperature tables, under 140 revs/min of rotating speeds, shaken cultivation obtains titanium pigment in 10 days.Wherein the volume ratio of without phosphorus culture medium and active sludge microorganism flora domestication culture fluid is 1:0.3, and the without phosphorus culture medium of every 1000ml adds 10g mid low grade phosphate rock.
In the mid low grade phosphate rock that the present embodiment adopts, the mass content of phosphorus pentoxide is 23.4%, and molten phosphorus rate reaches 73.1%.
Embodiment 4
A kind of method utilizing active sludge microorganism flora to dissolve mid low grade phosphate rock, comprises the steps:
1) enrichment culture of active sludge microorganism flora: phosphorus culture medium and activated sludge 1:0.4 by volume will be had to load in container (such as triangular flask), container is placed in 26 DEG C of constant-temperature tables under the rotating speed of 120 revs/min shaken cultivation 5 days, obtains the active sludge microorganism enriching floras culture fluid A of the 1st enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.3 by volume having phosphorus culture medium and the 1st enrichment culture is loaded in container, container is placed in 27 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 3 days, obtains the active sludge microorganism enriching floras culture fluid A of the 2nd enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.5 by volume having phosphorus culture medium and the 2nd enrichment culture is loaded in container, container is placed in 22 DEG C of constant-temperature tables under the rotating speed of 160 revs/min shaken cultivation 3 days, obtains active sludge microorganism enriching floras culture fluid (i.e. inoculation enrichment culture 3 times so repeatedly).
2) domestication of active sludge microorganism flora is cultivated: load in container (such as triangular flask) by without phosphorus culture medium and active sludge microorganism enriching floras culture fluid 1:0.2 by volume, 6g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 28 DEG C of constant-temperature tables under the rotating speed of 130 revs/min shaken cultivation 4 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 1st domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.22 by volume without phosphorus culture medium and the 1st domestication cultivated loads in container, 10g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 28 DEG C of constant-temperature tables under the rotating speed of 120 revs/min shaken cultivation 4 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 2nd domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.3 by volume without phosphorus culture medium and the 2nd domestication cultivated loads in container, 12g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 22 DEG C of constant-temperature tables under the rotating speed of 100 revs/min shaken cultivation 5 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 3rd domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.25 by volume without phosphorus culture medium and the 3rd domestication cultivated loads in container, 15g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 3 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 4th domestication is cultivated.
Without phosphorus culture medium and the 4th are tamed the active sludge microorganism flora domestication culture fluid B 1:0.3 by volume cultivated and loads in container, 18g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 30 DEG C of constant-temperature tables under the rotating speed of 120 revs/min shaken cultivation 4 days after mix homogeneously, obtains active sludge microorganism flora domestication culture fluid (i.e. inoculation domestication cultivation 5 times so repeatedly).
3) active sludge microorganism flora domestication culture fluid dissolves mid low grade phosphate rock: add in container by without phosphorus culture medium and mid low grade phosphate rock, add active sludge microorganism flora domestication culture fluid mix homogeneously, being placed in by container afterwards in 30 DEG C of constant-temperature tables, under 160 revs/min of rotating speeds, shaken cultivation obtains titanium pigment in 8 days.Wherein the volume ratio of without phosphorus culture medium and active sludge microorganism flora domestication culture fluid is 1:0.25, and the without phosphorus culture medium of every 1000ml adds 18g mid low grade phosphate rock.
In the mid low grade phosphate rock that the present embodiment adopts, the mass content of phosphorus pentoxide is 23.8%, and molten phosphorus rate reaches 69.6%.
Embodiment 5
A kind of method utilizing active sludge microorganism flora to dissolve mid low grade phosphate rock, comprises the steps:
1) enrichment culture of active sludge microorganism flora: phosphorus culture medium and activated sludge 1:0.4 by volume will be had to load in container (such as triangular flask), container is placed in 22 DEG C of constant-temperature tables under the rotating speed of 160 revs/min shaken cultivation 3 days, obtains the active sludge microorganism enriching floras culture fluid A of the 1st enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.3 by volume having phosphorus culture medium and the 1st enrichment culture is loaded in container, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 4 days, obtains the active sludge microorganism enriching floras culture fluid A of the 2nd enrichment culture.
The active sludge microorganism enriching floras culture fluid A 1:0.5 by volume having phosphorus culture medium and the 2nd enrichment culture is loaded in container, container is placed in 30 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 5 days, obtains active sludge microorganism enriching floras culture fluid (i.e. inoculation enrichment culture 3 times so repeatedly).
2) domestication of active sludge microorganism flora is cultivated: load in container (such as triangular flask) by without phosphorus culture medium and active sludge microorganism enriching floras culture fluid 1:0.3 by volume, 7g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 28 DEG C of constant-temperature tables under the rotating speed of 110 revs/min shaken cultivation 5 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 1st domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.2 by volume without phosphorus culture medium and the 1st domestication cultivated loads in container, 10g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 4 days after mix homogeneously, obtains the active sludge microorganism flora domestication culture fluid B that the 2nd domestication is cultivated.
The active sludge microorganism flora domestication culture fluid B 1:0.25 by volume without phosphorus culture medium and the 2nd domestication cultivated loads in container, 15g mid low grade phosphate rock is added to container again by the without phosphorus culture medium of every 1000ml, container is placed in 25 DEG C of constant-temperature tables under the rotating speed of 140 revs/min shaken cultivation 3 days after mix homogeneously, obtains active sludge microorganism flora domestication culture fluid (i.e. inoculation domestication cultivation 3 times so repeatedly).
3) active sludge microorganism flora domestication culture fluid dissolves mid low grade phosphate rock: add in container by without phosphorus culture medium and mid low grade phosphate rock, add active sludge microorganism flora domestication culture fluid mix homogeneously, being placed in by container afterwards in 25 DEG C of constant-temperature tables, under 140 revs/min of rotating speeds, shaken cultivation obtains titanium pigment in 10 days.Wherein the volume ratio of without phosphorus culture medium and active sludge microorganism flora domestication culture fluid is 1:0.3, and the without phosphorus culture medium of every 1000ml adds 15g mid low grade phosphate rock.
In the mid low grade phosphate rock that the present embodiment adopts, the mass content of phosphorus pentoxide is 20.6%, and molten phosphorus rate reaches 79.4%.
The bound of each raw material cited by the present invention, interval value, and the bound of technological parameter (such as temperature, time etc.), interval value can realize the present invention, embodiment numerous to list herein.
Claims (10)
1. one kind utilizes the method that active sludge microorganism flora dissolves mid low grade phosphate rock, it is characterized in that comprising the steps: 1) enrichment culture of active sludge microorganism flora: the activated sludge containing microorganism species is carried out enrichment culture, obtains active sludge microorganism enriching floras culture fluid；2) domestication of active sludge microorganism flora is cultivated: active sludge microorganism enriching floras culture fluid carries out domestication and cultivates, and obtains active sludge microorganism flora domestication culture fluid；3) active sludge microorganism flora domestication culture fluid dissolves mid low grade phosphate rock: without phosphorus culture medium and mid low grade phosphate rock are added in container, adds active sludge microorganism flora domestication culture fluid and cultivates.
2. as claimed in claim 1 a kind of utilize active sludge microorganism flora dissolve mid low grade phosphate rock method, it is characterized in that: step 1) enrichment culture of described active sludge microorganism flora includes a that phosphorus culture medium and activated sludge 1:0.3-0.5 by volume will be had to load in container, it is placed in constant-temperature table by container shaken cultivation, obtains active sludge microorganism enriching floras culture fluid A；B will have phosphorus culture medium and active sludge microorganism enriching floras culture fluid A 1:0.3-0.5 by volume to load in container, be placed in constant-temperature table by container shaken cultivation, the active sludge microorganism enriching floras culture fluid A being again enriched with；C repeats step b1-3 time, obtains active sludge microorganism enriching floras culture fluid.
3. as claimed in claim 2 a kind of utilize active sludge microorganism flora dissolve mid low grade phosphate rock method, it is characterised in that: constant-temperature table temperature is 22-30 DEG C, and rotating speed is 100-160 rev/min, and the shaken cultivation time is 3-5 days.
4. as claimed in claim 1 a kind of utilize active sludge microorganism flora dissolve mid low grade phosphate rock method, it is characterized in that: step 2) domestication of described active sludge microorganism enriching floras culture fluid cultivates and includes 1. loading in container by without phosphorus culture medium and active sludge microorganism enriching floras culture fluid 1:0.2-0.3 by volume, 5-10g mid low grade phosphate rock is added again by the without phosphorus culture medium of every 1000ml, it is placed in constant-temperature table by container after mix homogeneously shaken cultivation, obtains active sludge microorganism flora domestication culture fluid B；2. without phosphorus culture medium and active sludge microorganism flora are tamed culture fluid B 1:0.2-0.3 by volume and load in container, 10-15g mid low grade phosphate rock is added again by the without phosphorus culture medium of every 1000ml, it is placed in constant-temperature table by container after mix homogeneously shaken cultivation, the active sludge microorganism flora again tamed domestication culture fluid B；3. repeating step and 2. obtain active sludge microorganism flora domestication culture fluid for 1-3 time, gradually improve the content of mid low grade phosphate rock in container during domestication, last domestication is cultivated the without phosphorus culture medium of every 1000ml and is needed mid low grade phosphate rock 15-20g.
5. as claimed in claim 4 a kind of utilize active sludge microorganism flora dissolve mid low grade phosphate rock method, it is characterised in that: constant-temperature table temperature is 22-30 DEG C, and rotating speed is 100-140 rev/min, and the shaken cultivation time is 3-5 days.
6. as claimed in claim 1 a kind of utilize active sludge microorganism flora dissolve mid low grade phosphate rock method, it is characterized in that: step 3) described active sludge microorganism flora domestication culture fluid dissolves mid low grade phosphate rock and includes that without phosphorus culture medium and active sludge microorganism flora are tamed culture fluid 1:0.2-0.3 by volume and load in container, 10-20g mid low grade phosphate rock is added again by the without phosphorus culture medium of every 1000ml, container is placed in 22-30 DEG C of constant-temperature table after mix homogeneously, under 120-160 rev/min of rotating speed shaken cultivation 7-10 days.
7. a kind of method utilizing active sludge microorganism flora to dissolve mid low grade phosphate rock as claimed in claim 1, it is characterised in that: described activated sludge is from city domestic sewage processing plant.
8. as claimed in claim 1 a kind of utilize active sludge microorganism flora dissolve mid low grade phosphate rock method, it is characterised in that: described without phosphorus culture medium consists of 10g/L glucose, 0.5g/L yeast extract, 0.5g/L (NH4)2SO4、0.2g/LKCl、0.1g/LMgSO4·7H2O、0.0001g/LMnSO4·H2O、0.0001g/LFeSO4·7H2O, solvent is sterilized water.
9. as claimed in claim 2 a kind of utilize active sludge microorganism flora dissolve mid low grade phosphate rock method, it is characterised in that: described in have phosphorus culture medium to consist of 10g/L glucose, 0.5g/L yeast extract, 0.5g/L (NH4)2SO4、0.2g/LKCl、0.1g/LMgSO4·7H2O、0.0001g/LMnSO4·H2O、0.0001g/LFeSO4·7H2The calcium phosphate of O, 10-20g/L, solvent is sterilized water.
10. as claimed in claim 1 a kind of utilize active sludge microorganism flora dissolve mid low grade phosphate rock method, it is characterized in that: in described mid low grade phosphate rock, the mass content of phosphorus pentoxide is lower than 30%, it is 50-200 order that described mid low grade phosphate rock needs to be crushed to particle diameter before using.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108753615A (en) * | 2018-05-24 | 2018-11-06 | 江口县旭辉生态农业科技有限公司 | A method of dissolving low grade rock phosphate using Haloragidaceae Myriophyllum spicatum endophyte |
| CN115569974A (en) * | 2022-09-15 | 2023-01-06 | 湖北三峡实验室 | Method for progressive soil improvement of phosphogypsum storage yard and application thereof |
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| CN1348987A (en) * | 2001-12-02 | 2002-05-15 | 重庆和润实业(集团)有限公司 | Prepn of microbial colony sludge for efficient treatment of sewage |
| CN103952356A (en) * | 2014-04-29 | 2014-07-30 | 四川理工学院 | Method for preparing phosphate-solubilizing bacterium culture medium and phosphate-solubilizing bacterial manure |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1348987A (en) * | 2001-12-02 | 2002-05-15 | 重庆和润实业(集团)有限公司 | Prepn of microbial colony sludge for efficient treatment of sewage |
| CN103952356A (en) * | 2014-04-29 | 2014-07-30 | 四川理工学院 | Method for preparing phosphate-solubilizing bacterium culture medium and phosphate-solubilizing bacterial manure |
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| ALMUDENA RIVADENEYRA ET AL.: "Precipitation of Phosphate Minerals by Microorganisms Isolated from a Fixed-Biofilm Reactor Used for the Treatment of Domestic Wastewater", 《INT. J. ENVIRON. RES. PUBLIC HEALTH》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108753615A (en) * | 2018-05-24 | 2018-11-06 | 江口县旭辉生态农业科技有限公司 | A method of dissolving low grade rock phosphate using Haloragidaceae Myriophyllum spicatum endophyte |
| CN115569974A (en) * | 2022-09-15 | 2023-01-06 | 湖北三峡实验室 | Method for progressive soil improvement of phosphogypsum storage yard and application thereof |
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