CN1057430C - Biochemical rodenticide - Google Patents
Biochemical rodenticide Download PDFInfo
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- CN1057430C CN1057430C CN96117123A CN96117123A CN1057430C CN 1057430 C CN1057430 C CN 1057430C CN 96117123 A CN96117123 A CN 96117123A CN 96117123 A CN96117123 A CN 96117123A CN 1057430 C CN1057430 C CN 1057430C
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- botulinum toxin
- clostridium botulinum
- rodenticide
- biochemical
- biochemical rodenticide
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Abstract
A biochemical rodenticide comprises Ca type clostridium botulinum toxin or D type clostridium botulinum toxin, a flocculant, a buffering agent, sugar and water. The raticide overcomes the limitation that single clostridium botulinum toxin can only be used in high-cold areas having sparse people. The present invention has the characteristics of high efficiency, low toxicity and no residues; the biochemical rodenticide is safe for human and animals; poison bait prepared from base bait and the present invention can be used for killing various destructive mice in different habitats in various seasons.
Description
The present invention relates to a kind of large molecular toxicity albumen and kill the mouse composition, particularly relate to a kind of with C α type or the D type clostridium botulinum toxin biochemical rodenticide as active component.
Mouse suffers from harm, is of long duration. Along with population expansion, disturbed ecological balance, the phenomenon that the mouse mouth is growing on and on is on the rise. Over the past thousands of years, human just have the low molecular toxicity compound of utilization to prevent and treat the practice of muroid. Because natural low molecular toxicity effect lacks special selectively acting, palatability is poor, extracts difficulty, and the high in cost of production problem is so fail to develop always. Since the beginning of this century, artificial synthetic chemistry rat poison came out, because acute rodenticide is to the non-target animals poor stability, disabled or used by control. Chronic rat poison is slow because of toxic action again, and the expense of taking a lot of work material is difficult for being accepted by the masses. So the desirable rat poison of exploring efficient, low toxicity, noresidue is one of the important topic of subject that kills mouse always.
It is the pathogenic characteristic that some animal food is poisoned that China in 1985 utilizes C type clostridium botulinum toxin, utilize the different sensitiveness mechanism from common animals of muroid, at first design and tested C type clostridium botulinum toxin at the Alpine Grasslands Killing Pika, and succeeded. But because C type clostridium botulinum toxin reaches the stability problem that is difficult to keep virulence in warm plains region in safety issue densely populated, that the animal species complex area uses, do not have general directive significance. Therefore, for many years further popularization of this method has been subject to obstruction, fails to bring into play larger advantage.
The invention provides a kind of biochemical rodenticide, this agent as active component, has not only improved the security to people and animals with A α type or D type clostridium botulinum toxin, simultaneously half strong toxicity to muroid. The stabilization function auxiliary agents such as protective agent, anticorrisive agent of non-toxic component also are provided, have overcome the limitation that " merely " clostridium botulinum toxin can only use at extremely frigid zones, thereby enlarged the scope of application. Another object of the present invention has been improved the condition of preservation and the transportation of rat poison significantly, has strengthened practicality for adapting to the difference demand of killing mouse.
Technical scheme of the present invention
1, active ingredient
Clostridium botulinum toxin has seven kinds of different types such as A, B, C, D, E, F and G, and wherein only the C type is divided into again C α and two hypotypes of C β. Its bacterial strain type and toxin type have uniformity. The present invention proves by long-term inspection information and experimental study, exist the rule of different sensitivitys between toxin type and the animal species, various clostridium botulinum toxin is to the sensitiveness order of people and monkey, by strong to a little less than be followed successively by B＞A＞E＞C3＞F＞C α＞D type, wherein the gap of C α and D type clostridium botulinum toxin and C β type meat poisoning rib verticillium toxin is 100 and 330 times, reaches hundreds of times to thousands of times with the gap of A type clostridium botulinum toxin. Therefore, utilizing this characteristic of C α and D type clostridium botulinum toxin as the active component of rat poison, is to have desirable advantageous advantage (seeing Table) in known chemical toxin and biotoxin.
Show various clostridium botulinum toxin to monkey, people's sensitiveness
| Animal species | Give malicious approach | Toxic dose | Various clostridium botulinum toxin dosage (allen-Doisy unit) | ||||||
| B | A | E | Cβ | F | Cα | D | |||
| Monkey | 1. eating property | Mice lavage lethal dose/monkey per kilogram of body weight | 1.8 ×10 2 | 6.5 ×10 2 | 1.8 ×10 3 | 1.8 ×10 3 | 6.25 ×10 4 | 1.8 ×10 5 | 6 ×10 5 |
| The people | 2. eating property | (various countries' epidemiologic data) | ++++ | +++ | ++ | ± | + | - | - |
| Imbedibility | Lethal dose/people | 3. 20,000 | 4.＞6,000,000 | 5.＞3,000 ten thousand | |||||
| Annotate | " ++ ++ " owner cause of disease of will poisoning, " +++" be general poisoning cause of disease, two examples only occur in "+" so far, and " ± " not yet has definite data to prove, and "-" do not have the poisoning data. | ||||||||
| 1., Dolman C.E.and Marailami L.:J.Infect.Dis.109/2:107.1961. 2., the work such as summer grand plan, botulismus, the Xinjiang People's Press publishes, nineteen eighty-two, the 108th page 3., Yu He etc.: Medical Microbiology, the People's Health Publisher publishes, nineteen eighty-three, the 483rd page. 4., be inventor's testing data. | |||||||||
2, stabilizing agent
C α and D type clostridium botulinum toxin belong to the macromolecule toxic protein, and be comparatively responsive to chemical factors such as temperature, light radiation, humidity, pH value, pernicious gases (oxygen), easily causes rapid mistake poison. The present invention by reducing agent and buffer and the sugar of natural organic high-molecular flocculant, low molecular compound, and the effect of anticorrisive agent, will significantly improve the stability of active component through test.
3, realize the technical scheme of the object of the invention
(1), allen-Doisy unit and mensuration thereof
The virulence criterion of C α and D type clostridium botulinum toxin can be used " allen-Doisy unit " (mouse unit, M.U.) expression. The present invention's regulation is calculated as radix as the minimum lethal dose (MLD) of 14～16 gram small white mouses take clostridium botulinum toxin intravenous injection body weight, and namely 1 MLD is 1 MU.
(2), concrete technical scheme
A kind of biochemical rodenticide contains C α type clostridium botulinum toxin or D type clostridium botulinum toxin, flocculant, buffer, candy and water. Also contain reducing agent, anticorrisive agent.
Flocculant is gelatin, pectin, starch, skimmed milk.
Buffer is sodium hydrogen phosphate, potassium dihydrogen phosphate, sodium glutamate.
Sugar is sucrose, glucose.
Reducing agent is cysteine, sodium thioglycollate, vitamin C.
Anticorrisive agent is the mixture of sorbic acid or penicillin, streptomysin.
Flocculant, buffer, sugar are made into the aqueous solution, mix with C α or D type clostridium botulinum toxin, make in per hundred ml mixtures to contain 0.1 * 107～20×10
8MUC α or D type clostridium botulinum toxin, 0.2～8g flocculant, 0.1～8g buffer, 0.5～10g sugar.
Also contain 0.04～0.1g reducing agent, 0.01～0.1g (or 2～200,000 units) anticorrisive agent in per hundred milliliters of rat poisons. When anticorrisive agent was sorbic acid, its amount was 0.01～0.1g; When anticorrisive agent was the mixture of penicillin, streptomysin, its amount was 2～200,000 units.
(3), the making of biochemical rodenticide
In proportion, with the various compositions of stabilizing agent, process through autoclaving, be incorporated in successively in C α or the D type clostridium botulinum toxin, make it to meet desired content, and frequently slowly shake toxin, evenly mixed, prevent that grumeleuse from occurring. Wherein, when gelatine content is 0.2～5g in per hundred ml mixtures, be the aqua formulation. It is the gelling agent formulation when every milliliter of gelatine content is 5～8%. With gelling agent rat poison, behind vacuum freeze-drying technique, be the dry freeze agent. Product is made rear sealing lucifuge and is stored.
Technical characterstic of the present invention and implementation result
1, wide, the strong toxicity of target spectrum
C α type and D type clostridium botulinum toxin are respectively 250～500MU to the main bandicoot gavage lethal dose such as Apodemus agrarius, Microtus mandarinus, Rattus norvegicus, Rattusflauipectus, with 500～1500MU, through the grassland, farmland, city more than ten field trial of planting common bandicoot, above-mentioned toxin is all responsive, and demonstrates enough virulence.
2, person poultry safety
Evidence, C α type clostridium botulinum toxin to duck per os lethal dose greater than 105MU, to chicken, cat, dog, pig greater than 106MU. Especially C α and D type clostridium botulinum toxin, than large 100 and 333 times of C β type clostridium botulinum toxin, large more hundreds of to thousands of times than A, Type B clostridium botulinum toxin to the degree of safety of people and monkey. According to epidemiology and laboratory data, C α type clostridium botulinum toxin is in the area without aquatic bird, little body birds, D type clostridium botulinum toxin is in the area without ox, ermine, and it is little to other non-target animals danger to press the concentration that poison bait uses, and without secondary poisoning may. This existing chemical rat poison can't be compared.
3, good palatability
The coefficient standard of ingesting of general rat poison is for greater than 0.3, and C α type meat poisoning shuttle toxin poison bait is respectively 1.12 and 0.61 to the coefficient of ingesting of Rattus norvegicus, Rattusflauipectus. The former poisonous poison bait uptake ratio is greater than nontoxic poison bait.
4, subacute poisoning effect
Botulinum toxin is typical neural numb low toxin, and the poisoning muroid presents muscle fiber crops low, quadriplegia, because respiratory disorder causes death. Its latent period of poisoning 1～2 day, 2～4 days death times. This " subacute " course of disease is conducive to the colony's control to muroid, and unique critical role is arranged in present chemical rat poison, meets the development need of dwindling acute and chronic rat poison boundary.
5, protection Natural Enemies of Rats, maintaining ecological balance
Facts have proved that the Natural Enemies of Rats such as hawk, fox, cat, dog have stronger resistance to C α and D type clostridium botulinum toxin. The toxin poison bait is difficult for contaminated environment at open condition weak point of lower residual life, and maintaining ecological balance is had important strategic importance.
6, spot effect
For many years, biochemical rodenticide through the grassland, farmland, Urban Areas have more than 200 ten thousand mu of field trials and confirm, reaches 85～100% at trial mouse kind average kill ratio, people and animals' intoxication accident is found at the end. Owing to remedied the deficiency of existing acute and chronic chemical rat poison, have a saving of labor, save time, material-saving, efficient, safe characteristics. Along with enforcement of the present invention, it is close with acute rodenticide that its expenses for prevention and control is starkly lower than chronic rat poison, will bring into play larger social benefit, economic benefit and ecological benefits.
7, applied widely
Not only can use in high and cold, lonely area, but also can be in warm plains region and densely populated, animal species complex area use.
Embodiment 1:
Make the rat poison of active component with C α type clostridium botulinum toxin.
Get respectively C α type clostridium botulinum toxin, gelatin, sodium hydrogen phosphate, potassium dihydrogen phosphate, sucrose, penicillin, streptomysin.
Gelatin, sodium hydrogen phosphate, potassium dihydrogen phosphate, sucrose are made into respectively the aqueous solution, stand-by behind the autoclaving. Penicillin, streptomysin is rear stand-by with the sterile purified water dilution respectively.
Above-mentioned all aqueous solution are joined in the C α type clostridium botulinum toxin, make in per hundred milliliters the mixture to contain 0.1 * 107MUC α type boticin element, 0.2g gelatin, 0.3g sodium hydrogen phosphate, 0.1g potassium dihydrogen phosphate, 1g sucrose, 20,000 unit penicillin, 20,000 unit streptomysins after fully mixing, namely make watery biochemical rodenticide.
The toxin poison bait that this agent is prepared has the mouse of killing to be renderd a service by force, does not jeopardize people and animals, especially suits to use in the area without aquatic bird, corpusculum shape birds. In addition, increased atoxic functional aid in this agent, having overcome simple clostridium botulinum toxin can only in the limitation of extremely frigid zones use, enlarge the scope of application.
Embodiment 2:
Get respectively C α type clostridium botulinum toxin, gelatin, sodium hydrogen phosphate, potassium dihydrogen phosphate, sucrose, penicillin, streptomysin.
By embodiment 1 method of chatting preparation, and make in per hundred milliliters of the rat poisons made and contain 5 * 108The pretty verticillium toxin of MUC α type meat poisoning, 6g gelatin, 5.5g sodium hydrogen phosphate, in contain 5 * 108The pretty verticillium toxin of MUC α type meat poisoning, 6g gelatin, 5.5g sodium hydrogen phosphate, 2.5g potassium dihydrogen phosphate, 8g sucrose, 50,000 unit penicillin, 50,000 unit streptomysins are made biochemical rodenticide as a form of gel.
The rat poison of making kills mouse and renders a service height, does not jeopardize the people and holds, and especially suits to use in the area without aquatic bird, corpusculum shape birds. In addition, increased atoxic functional aid in this agent, having overcome simple clostridium botulinum toxin can only in the limitation of extremely frigid zones use, enlarge the scope of application. And volume is little, is convenient to transportation, preserves.
Embodiment 3:
Get respectively C α type clostridium botulinum toxin, gelatin, sodium hydrogen phosphate, potassium dihydrogen phosphate, sucrose, penicillin, streptomycin.
By the preparation of front method, and make in per hundred milliliters of the said preparations and contain 5 * 108MUC α type clostridium botulinum toxin, 6g gelatin, 6g sodium hydrogen phosphate, 2g potassium dihydrogen phosphate, 10g sucrose, 50,000 unit penicillin, 50,000 unit streptomysins. Through vacuum freeze-drying technique, namely make the block biochemical rodenticide of cake again.
The rat poison result of use of making and precedent are together. And volume is little, and is lightweight, long shelf-life.
Embodiment 4:
Get respectively D type clostridium botulinum toxin, gelatin, sodium hydrogen phosphate, potassium dihydrogen phosphate, sucrose, penicillin, streptomysin.
By the preparation of front method, and make in per hundred milliliters of the said preparations and contain 4 * 106MUD type clostridium botulinum toxin, 0.2g gelatin, 0.3g sodium hydrogen phosphate, 0.1g potassium dihydrogen phosphate, 1g sucrose, 20,000 unit penicillin, 20,000 unit streptomysins. Namely make watery biochemical rodenticide.
The rat poison of making is without ermine, ox and ruminate beastly area and use, and effect and precedent are same.
Embodiment 5:
Get respectively D type clostridium botulinum toxin, gelatin, sodium hydrogen phosphate, potassium dihydrogen phosphate, sucrose, penicillin, streptomysin.
By the preparation of front method, and contain 2 * 10 in per hundred milliliters of the rat poisons of making9MUD type clostridium botulinum toxin, 8g gelatin, 7.5g sodium hydrogen phosphate, 2.5g potassium dihydrogen phosphate, 10g sucrose, 200,000 unit penicillin, 200,000 unit streptomysins. Namely make biochemical rodenticide as a form of gel.
Effect and example 4 are same, and volume is little, is convenient to transportation, preserves.
Embodiment 6:
Get respectively D type clostridium botulinum toxin, gelatin, sodium hydrogen phosphate, potassium dihydrogen phosphate, sucrose, penicillin, streptomysin.
By the preparation of front method, make in per hundred milliliters of the rat poisons made to contain 2 * 109MUD type clostridium botulinum toxin, 6g gelatin, 6g sodium hydrogen phosphate, 2g potassium dihydrogen phosphate, 200,000 unit penicillin, 200,000 unit streptomysins.
The rat poison of making is as a form of gel. Through vacuum freeze-drying technique, namely make the block biochemical rodenticide of cake again. Effect and example 4 are same, and volume is little, and be lightweight, long shelf-life.
Claims (8)
1, a kind of biochemical rodenticide contains C α type clostridium botulinum toxin or D type clostridium botulinum toxin, flocculant, buffer, sugar and water.
2, the biochemical rodenticide of narrating according to claim 1 is characterized in that also containing reducing agent, anticorrisive agent.
3, the biochemical rodenticide of narrating according to claim 1 is characterized in that flocculant is gelatin, pectin, starch, skimmed milk.
4, the biochemical rodenticide of narrating according to claim 1 is characterized in that buffer is sodium hydrogen phosphate, potassium dihydrogen phosphate, sodium glutamate.
5, the biochemical rodenticide of narrating according to claim 2 is characterized in that reducing agent is cysteine, sodium thioglycollate, vitamin C.
6, the biochemical rodenticide of narrating according to claim 2 is characterized in that anticorrisive agent is the mixture of sorbic acid or penicillin, streptomysin.
7, the biochemical rodenticide of narrating according to claim 1 is characterized in that flocculant, buffer, sugar are made into the aqueous solution, mixes with clostridium botulinum toxin, contains 0.1 * 10 in per hundred ml mixtures7～20×10
8The MLD clostridium botulinum toxin, 0.2～8g flocculant, 0.1～8g buffer, 0.5～10g sugar.
8, the biochemical rodenticide of narrating according to claim 2 is characterized in that also containing in per hundred milliliters of rat poisons 0.01～0.1g reducing agent, 0.01～0.1g (or 2～200,000 units) anticorrisive agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96117123A CN1057430C (en) | 1996-10-09 | 1996-10-09 | Biochemical rodenticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96117123A CN1057430C (en) | 1996-10-09 | 1996-10-09 | Biochemical rodenticide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1164331A CN1164331A (en) | 1997-11-12 |
| CN1057430C true CN1057430C (en) | 2000-10-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96117123A Expired - Fee Related CN1057430C (en) | 1996-10-09 | 1996-10-09 | Biochemical rodenticide |
Country Status (1)
| Country | Link |
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| CN (1) | CN1057430C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW574036B (en) * | 1998-09-11 | 2004-02-01 | Elan Pharm Inc | Stable liquid compositions of botulinum toxin |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87103514A (en) * | 1987-05-10 | 1988-07-13 | 沈世英 | Biological toxin raticide |
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1996
- 1996-10-09 CN CN96117123A patent/CN1057430C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87103514A (en) * | 1987-05-10 | 1988-07-13 | 沈世英 | Biological toxin raticide |
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| Publication number | Publication date |
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| CN1164331A (en) | 1997-11-12 |
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Owner name: YANGZHOU CUISHI BIOLOGY LABORATORY CO., LTD. Free format text: FORMER OWNER: CUI SHENGFA Effective date: 20040521 |
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Effective date of registration: 20040521 Address after: 225000 No. 5 East Garden Road, Jiangsu, Yangzhou Patentee after: Yangzhou Cuishi biological laboratories Co. Ltd. Address before: Jiangsu province Yangzhou City Road 225001 Yanfu No. 1 box 41 Patentee before: Cui Shengfa |
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Granted publication date: 20001018 Termination date: 20111009 |