CN105738183A - Method for detecting annual ring of desert plant haloxylon - Google Patents
Method for detecting annual ring of desert plant haloxylon Download PDFInfo
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- CN105738183A CN105738183A CN201610113824.9A CN201610113824A CN105738183A CN 105738183 A CN105738183 A CN 105738183A CN 201610113824 A CN201610113824 A CN 201610113824A CN 105738183 A CN105738183 A CN 105738183A
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- China
- Prior art keywords
- alkaloid
- annual ring
- developer
- bismuth
- acid
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- 238000000034 method Methods 0.000 title claims abstract description 15
- 241001632080 Haloxylon Species 0.000 title abstract 7
- 229930013930 alkaloid Natural products 0.000 claims abstract description 32
- 150000003797 alkaloid derivatives Chemical class 0.000 claims abstract description 32
- JCXGWMGPZLAOME-UHFFFAOYSA-N bismuth atom Chemical compound [Bi] JCXGWMGPZLAOME-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000001514 detection method Methods 0.000 claims abstract description 7
- 150000001621 bismuth Chemical class 0.000 claims abstract description 5
- 229910052797 bismuth Inorganic materials 0.000 claims abstract description 5
- 239000002253 acid Substances 0.000 claims abstract description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 14
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 12
- RXPAJWPEYBDXOG-UHFFFAOYSA-N hydron;methyl 4-methoxypyridine-2-carboxylate;chloride Chemical compound Cl.COC(=O)C1=CC(OC)=CC=N1 RXPAJWPEYBDXOG-UHFFFAOYSA-N 0.000 claims description 9
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 8
- 229910017604 nitric acid Inorganic materials 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000010413 mother solution Substances 0.000 claims description 6
- 229960000583 acetic acid Drugs 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000012362 glacial acetic acid Substances 0.000 claims description 3
- RPAJSBKBKSSMLJ-DFWYDOINSA-N (2s)-2-aminopentanedioic acid;hydrochloride Chemical compound Cl.OC(=O)[C@@H](N)CCC(O)=O RPAJSBKBKSSMLJ-DFWYDOINSA-N 0.000 claims description 2
- QYIGOGBGVKONDY-UHFFFAOYSA-N 1-(2-bromo-5-chlorophenyl)-3-methylpyrazole Chemical compound N1=C(C)C=CN1C1=CC(Cl)=CC=C1Br QYIGOGBGVKONDY-UHFFFAOYSA-N 0.000 claims description 2
- YZCKVEUIGOORGS-UHFFFAOYSA-N Hydrogen atom Chemical compound [H] YZCKVEUIGOORGS-UHFFFAOYSA-N 0.000 claims description 2
- 239000011260 aqueous acid Substances 0.000 claims description 2
- JHXKRIRFYBPWGE-UHFFFAOYSA-K bismuth chloride Chemical compound Cl[Bi](Cl)Cl JHXKRIRFYBPWGE-UHFFFAOYSA-K 0.000 claims description 2
- 229910000380 bismuth sulfate Inorganic materials 0.000 claims description 2
- BEQZMQXCOWIHRY-UHFFFAOYSA-H dibismuth;trisulfate Chemical compound [Bi+3].[Bi+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O BEQZMQXCOWIHRY-UHFFFAOYSA-H 0.000 claims description 2
- 235000006408 oxalic acid Nutrition 0.000 claims description 2
- 239000001117 sulphuric acid Substances 0.000 claims description 2
- 235000011149 sulphuric acid Nutrition 0.000 claims description 2
- 230000004075 alteration Effects 0.000 abstract description 3
- 239000003513 alkali Substances 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 abstract 3
- 150000007513 acids Chemical class 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000011248 coating agent Substances 0.000 abstract 1
- 238000000576 coating method Methods 0.000 abstract 1
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 abstract 1
- 238000005498 polishing Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 5
- 239000004575 stone Substances 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
Abstract
The invention provides a method for detecting an annual ring of a desert plant haloxylon. According to the method, the annual ring of the haloxylon can be accurately measured, and the method specifically comprises the following detection steps: A, detecting a haloxylon standard sample, namely taking a haloxylon trunk growing in desert and gobi, cutting a flake sample, and polishing the sample to be smooth for later use; B, coating an alkaloid color developing agent on the sample, and acquiring the annual ring data after a clear annual ring appears on the sample surface. Alkali in the haloxylon is combined with the alkaloid color developing agent to present brownish red; the alkaloid color developing agent is an aqueous solution of bismuth salts and acids, wherein the content of bismuth element is 0.0001-0.04mol/L, and the concentration of hydrogen ion is 1.3-3.5mol/L. According to the detection method, chromatic aberration occurs in the trunk section, and the annual ring of the haloxylon can be clearly read out under a microscope.
Description
Technical field
The present invention relates to a kind of method detecting desert plant sacsaoul annual ring, platymiscium field.
Background technology
A kind of shrub sacsaoul of growth in desert and Gobi.Detection determines that the annual ring of sacsaoul is to decoding Laws of Climate Change, understands local decades, the history of centuries climate change is of great significance.Due to desert and Gobi Extreme drought, plant growing condition is severe, and sacsaoul grows slowly.One sacsaoul that grown decades, centuries, trunk only has as finger thick.Common trunk annual ring is clearly denumerable, can interpret the climate change history of locality according to the quantity of annual ring and width.Sacsaoul is due to poor growth, and phloem is very thin, and trunk cross section color zero difference does not see annual ring yet, is difficult to calculate the age of tree according to annual ring, more cannot decode climate change history according to annual ring.The detection of sacsaoul annual ring is one world-famous puzzle to have scholar to think.The climate change history of desert and Gobi centuries, rule are the important references of neoclimatology research, so the detection of sacsaoul annual ring, the determination of the age of tree is particularly significant.
Summary of the invention
It is an object of the invention to provide a kind of method detecting desert plant sacsaoul annual ring, the method can determine the annual ring of sacsaoul exactly, and concrete detecting step is as follows:
A, sacsaoul detection standard specimen: take the sacsaoul trunk of growth, cutting sheet sample in desert and Gobi, then sample is polished smooth stand-by；
B, alkaloid developer is applied on sample, at 30～45 DEG C, constant temperature 25～40 minutes, occur, clearly after annual ring, gathering annual ring data until specimen surface；It is shown as brownish red after being combined with alkaloid developer due to the alkali in sacsaoul；After alkaloid developer is applied on sample, it is preferable that at 35 DEG C, constant temperature 30 minutes.
Described alkaloid developer is bismuth salt and aqueous acid, and wherein the concentration of bismuth element is 0.0001～0.04mol/L, and hydrionic concentration is 1.3～3.5mol/L.
Containing wood nicotinoids alkaloid in sacsaoul trunk, alkaloidal content is relevant to the change of weather, changes in non-homogeneous.The phloem of sacsaoul is very thin, and this is the evolution result that plant resists desert Extreme drought.Phloem internal layer is cambium layer, is the growing point of xylem.The alkaloid of phloem internal layer and the alkaloid of xylem there are differences.
Owing to the alkaloid of phloem internal layer Yu xylem there are differences, and in sacsaoul xylem, alkaloid there is also uneven, and after utilizing the dyeing of alkaloid developer, trunk cross section presents aberration, can clearly read the annual ring of sacsaoul under the microscope.
Sacsaoul annual ring is after the display, it is possible to take sample annual ring image with the digital camera on biological microscope, to be convenient to scientific research.The age of tree can be extrapolated, it is possible to decode local centuries climate change history according to the change of annual ring according to annual ring.
Further, described bismuth salt is the one in bismuth nitrate, bismuth sulfate, bismuth acetate or bismuth chloride；Described acid is the one in sulphuric acid, acetic acid, nitric acid, hydrochloric acid, carbolic acid or oxalic acid.
Adopt the alkaloid developer that above-mentioned bismuth salt and acid are allocated, it is possible to make trunk cross section present aberration preferably, be conducive to the identification of annual ring.
The configuration of alkaloid developer preferably first configures alkaloid developer mother solution, and containing bismuth element 0.0002～0.08mol/L, hydrion 2.6～7.0mol/L in alkaloid developer mother solution, all the other compositions are water；In use, first by alkaloid developer mother solution dilute one times, it is modulated to alkaloid developer, then re-uses.
The optimization formula of alkaloid developer has following two:
One, by bismuth nitrate 50mg, mass percent is the concentrated nitric acid 20ml of 65%, 5% dilute sulfuric acid 80ml, the proportions of distilled water 100ml.
Two, by bismuth nitrate 50mg, mass percent is 65% concentrated nitric acid 20ml, glacial acetic acid 10ml, 5% dilute sulfuric acid 70ml, the proportions of distilled water 100ml.
Detailed description of the invention
Embodiment 1:
Take desert and Gobi arid place sacsaoul trunk, be cut into the disk of thick 0.5 centimetre with abrasive wheel cutting machine, with fine oil stone, tangent plane is polished as sample.
Weigh bismuth nitrate 50mg, add the nitric acid 20ml that mass percent is 65%, make bismuth salt dissolve, be subsequently adding 5% dilute sulfuric acid 80ml, mix homogeneously.Measure 50ml, add 50ml distilled water, mix homogeneously, be configured to alkaloid developer.
With the fine, soft fur pen that polrvinyl chloride is material, alkaloid developer is uniformly painted on sample, puts in calorstat, react 30 minutes at 35 DEG C, take out sample under biological microscope, detect annual ring.
Embodiment 2:
Take desert and Gobi the most arid place shrub sacsaoul, extract the disk of trunk 0.5 cm thick, with fine oil stone, disk is polished smooth as sample.
Accurately weighing bismuth nitrate 50mg, concentrated nitric acid 20ml, put in conical flask, jolting makes bismuth nitrate dissolve gently, adds glacial acetic acid 10ml, 5% dilute sulfuric acid 70ml, jolting mix homogeneously.Measuring 50ml, add distilled water 50ml, mix homogeneously, for the special developer of sacsaoul alkaloid.
With fine, soft fur pen, developer is uniformly painted on sample, 35 DEG C of constant temperature 30 minutes, moves under biological microscope, the annual ring of tree can be detected.Shoot the annual ring image on sample with the mutatis mutandis digital camera on biological microscope, unloading in computer through after can from the history of research deciphering climate change annual ring spacing.
Claims (7)
1. the method detecting desert plant sacsaoul annual ring, comprises the steps:
A, sacsaoul detection standard specimen: take the sacsaoul trunk of growth, cutting sheet sample in desert and Gobi, then sample is polished smooth stand-by；
B, alkaloid developer is applied on sample, at 30～45 DEG C, constant temperature 25～40 minutes, occur, clearly after annual ring, gathering annual ring data until specimen surface；
Described alkaloid developer is bismuth salt and aqueous acid, and wherein the concentration of bismuth element is 0.0001～0.04mol/L, and hydrionic concentration is 1.3～3.5mol/L.
2. method according to claim 1, it is characterised in that described bismuth salt is the one in bismuth nitrate, bismuth sulfate, bismuth acetate or bismuth chloride.
3. method according to claim 1, it is characterised in that described acid is at least one in sulphuric acid, acetic acid, nitric acid, hydrochloric acid, carbolic acid or oxalic acid.
4. method according to claim 1, it is characterised in that in stepb, after alkaloid developer is applied on sample, at 35 DEG C, constant temperature 30 minutes.
5. method according to claim 1, it is characterised in that also including prefabricated alkaloid developer mother solution, alkaloid developer mother solution contains bismuth element 0.0002～0.08mol/L, hydrion 2.6～7.0mol/L, and all the other compositions are water；In use, by alkaloid developer mother solution dilute one times, it is modulated to alkaloid developer.
6. method according to claim 1, it is characterised in that bismuth nitrate 50mg pressed by alkaloid developer, mass percent is the concentrated nitric acid 20ml of 65%, 5% dilute sulfuric acid 80ml, the proportions of distilled water 100ml.
7. method according to claim 1, it is characterised in that bismuth nitrate 50mg pressed by alkaloid developer, mass percent is 65% concentrated nitric acid 20ml, glacial acetic acid 10ml, 5% dilute sulfuric acid 70ml, the proportions of distilled water 100ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610113824.9A CN105738183A (en) | 2016-02-29 | 2016-02-29 | Method for detecting annual ring of desert plant haloxylon |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610113824.9A CN105738183A (en) | 2016-02-29 | 2016-02-29 | Method for detecting annual ring of desert plant haloxylon |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105738183A true CN105738183A (en) | 2016-07-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610113824.9A Pending CN105738183A (en) | 2016-02-29 | 2016-02-29 | Method for detecting annual ring of desert plant haloxylon |
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| Country | Link |
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| CN (1) | CN105738183A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111413333A (en) * | 2020-04-23 | 2020-07-14 | 北华大学 | Preparation method of coloring agent for assisting in distinguishing annual discs of trees |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1046832A (en) * | 1990-06-27 | 1990-11-14 | 中国中医研究院中药研究所 | Judge the new method of the age of tree |
| CN102451220A (en) * | 2010-10-26 | 2012-05-16 | 中国医学科学院药物研究所 | Erythrophleum fordii Oliver extract, preparation method and pharmaceutical composition thereof and application |
| CN102749330A (en) * | 2012-05-08 | 2012-10-24 | 冯泽民 | Method for calculating tree age and annual ring width by using ordinary camera |
| CN103940738A (en) * | 2013-01-17 | 2014-07-23 | 复旦大学 | Method for quickly identifying ginseng age |
-
2016
- 2016-02-29 CN CN201610113824.9A patent/CN105738183A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1046832A (en) * | 1990-06-27 | 1990-11-14 | 中国中医研究院中药研究所 | Judge the new method of the age of tree |
| CN102451220A (en) * | 2010-10-26 | 2012-05-16 | 中国医学科学院药物研究所 | Erythrophleum fordii Oliver extract, preparation method and pharmaceutical composition thereof and application |
| CN102749330A (en) * | 2012-05-08 | 2012-10-24 | 冯泽民 | Method for calculating tree age and annual ring width by using ordinary camera |
| CN103940738A (en) * | 2013-01-17 | 2014-07-23 | 复旦大学 | Method for quickly identifying ginseng age |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111413333A (en) * | 2020-04-23 | 2020-07-14 | 北华大学 | Preparation method of coloring agent for assisting in distinguishing annual discs of trees |
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Application publication date: 20160706 |