CN105727857A - Microfluidic apparatus produced by 3D printing - Google Patents
Microfluidic apparatus produced by 3D printing Download PDFInfo
- Publication number
- CN105727857A CN105727857A CN201410747264.3A CN201410747264A CN105727857A CN 105727857 A CN105727857 A CN 105727857A CN 201410747264 A CN201410747264 A CN 201410747264A CN 105727857 A CN105727857 A CN 105727857A
- Authority
- CN
- China
- Prior art keywords
- aqueous phase
- emulsion
- pipe
- printing
- microfluidic device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a microfluidic apparatus used for the continuous preparation of a "water-in-oil-in-water" (W/O/W) double-emulsion. The apparatus is produced by the 3D printing technology. The apparatus comprises an internal water phase tube 1, an oil phase tube 2, an external water tube 3 and an emulsion collecting tube 4. The internal water phase tube and the oil tube are of a coaxial taper tube structure; the external water phase tube and the emulsion tube are of a reverse coaxial structure; the flowing section smoothly transits from large to small; and the formation condition of the "water-in-oil-in-water double-emulsion" is met. The diameter of the internal water phase flow and the thickness of the oil phase flow can achieve 0.2 to 0.1 mm; the caliber of the emulsion collecting tube is in the range of 1.0 to 0.5 mm. A standardize hose coupler is applied for the connection between the apparatus and the outside.
Description
Technical field
The present invention relates to a kind of microfluidic device preparing microcapsule, this device particularly made with 3D printing technique, belong to nano science and technical field of medicine preparation.
Background technology
Micro-fluidic technologies early has application in scientific instrument, for instance cell is dispersed in aqueous medium by the principle of flow cytometer exactly, allows cell dispersion liquid flow through a very thin capillary tube, only allows cell to pass through singly, thus realizing detection and the sorting of cell.Development along with Nanometer scale science and technology, microfluid shows new vitality, become the important method preparing micron and nanometer bead and vesicle, correspondingly, the flowing of microfluid, mixing, surface and interface behavior obtain deep research, having had significant progress, the device of various acquisition microfluids also arises at the historic moment.These devices mainly divide two big classes.The fluid passage of one class is to be formed by the method for etching, its feature is that runner is very thin, in micron dimension, thus the granule prepared with it is up to nanosized, and uniformly, but its manufacturing process is complicated, and costly, microchannel is transferred to conventional pipeline and has a lot of difficulty technically, thus the method is difficult to universal and industry amplification.The another kind of method acquisition fluid passage by machining, is typically in millimeter to sub-millimeter meter range, thus the microsphere obtained or capsule is typically in the magnitude of tens to hundreds of micron.How to process thinner runner, it is thus achieved that less particle diameter, be the key point of the method Future Development.Except reducing the aperture of runner, utilize the hydrodynamic characteristic of fluid and microfluid, carry out the particular design in microfluid pond, and micro-fluidic technologies is combined with self-assembling technique, be all the possible approaches obtaining less particle diameter, but the report of this respect is little.
The development of zero-dimension nano technology, after all, it is simply that the preparation of micron or nanoparticle, micelle and capsule and application.Wherein micron and Nano capsule tool are of great significance.First, it can bag medicine because the relative volume of its interior aqueous phase is very big, it is possible to hold water miscible medicine, particularly polypeptide, protein and nucleic acid drug, and this kind of medicine suitable supports delivery system but without finding so far.Secondly, micron and Nano capsule are the best simulations to cell wall, and the physicochemical property of research micron and Nano capsule contributes to people and deepens the understanding to cell behavior, explores more secrets of life.3rd, micron and Nano capsule be a kind of microreactor still, people study the various chemical reactions in micron and Nano capsule, particularly relevant with vital movement reaction, such as mould short synthesis and enzymatic hydrolysis, not only can deepen the understanding to life process, it is also possible to create the chemical reaction process of more efficient more environmental protection.
The method that the preparation of micron and Nano capsule is generally adopted " emulsifying ", namely interior aqueous phase solution emulsion in oil-phase solution it is initially formed, then by this emulsion dispersion to aqueous medium i.e. outer aqueous phase, " W/O/W (W/O/W) " emulsion is formed, also known as " double; two emulsion ".The shortcoming of this method is when second time is scattered, oil-phase solution is broken into dispersion phase from continuous phase, interior aqueous phase and outer aqueous phase tend to occur merge in this process, what obtain is really O/W emulsion or O/W, W/O/W mixture, medicine in interior aqueous phase is often gone in outer aqueous phase, and bag drug effect rate is very low.In order to avoid the fusion of inside and outside aqueous phase, people's imagination is when the first step forms W/O, and oil phase crust is very thin, it is not necessary to is crushed with very big energy and can form W/O/W emulsion.Wherein, dynamic continuous emulsification is exactly a kind of successfully trial.Its principle is shown in Fig. 1.Interior aqueous phase and oil phase coaxially flow, and due to capillary effect, form double-deck liquid pearl in exit, and under the drive of the outer aqueous phase that quickly flows, liquid pearl elongates occurs that the fracture of thin neck, thin neck becomes vesicle, continues to move ahead, passes through pipe-type outlet.In laboratory conditions, the size of the outlet of above-mentioned interior aqueous phase, oil phase outlet and emulsion inlet can accomplish about 1mm, and experiment proves successful, and the vesicle external diameter obtained is about about 200 μm.Experiment also confirms that: the coaxial in the same direction faster flow velocity relative to outer aqueous phase that flow of interior aqueous phase, oil phase and the local of outer aqueous phase, is the essential condition forming W/O/W emulsion, and the size of formed emulsion droplet may diminish to 1/5 to the 1/10 of outlet size.The vesicle prepared by dynamic continuity method of current bibliographical information, size is mostly at 200～100 μ m.Due to the restriction of processing method, the outlet size of interior aqueous phase and oil phase pipeline is difficult to do little, and the relative velocity of outer aqueous phase is difficult to improve further.
Three-dimensional (3D) prints is one of most efficient method preparing nanostructured, development at full speed has been obtained since this century, various 3D printing techniques and relevant device are succeeded in developing and commercialization, metal, plastics, monomer etc. can print, the precision printed has reached 0.1mm(plastics and has printed) or micron dimension (photosensitive resin printings), thus can processing structure is complicated, required precision height, machining and injection mo(u)lding cannot complete object.This provides possibility for development machining class micro-fluidic technologies.
Summary of the invention
Present disclosure, it is simply that 3D printing technique is applied to the manufacture of microfluidic device, forms special flow passage structure, in order to obtains less particle diameter, and realizes multichannel input and the output of fluid by simple method.
Principle and bibliographical information are known as shown in Figure 1: the adjustable parameters of the method has: the composition of (1) interior aqueous phase, oil phase and outer aqueous phase and concentration, and by forming the viscosity and surface tension that determine with concentration, (2) interior aqueous phase pipe 1, oil phase pipe 2, outer aqueous phase pipe 3 and the geometry of emulsion collecting pipe 4 binding site, size and relative position, (3) flow velocity of interior aqueous phase, oil phase and outer aqueous phase, the hydrophilic and hydrophobic on (4) above-mentioned piping material surface.The invention mainly relates to (2nd) and (3rd) item, namely how to be printed by 3D, obtain the interior aqueous phase pipe 1 of precision, oil phase pipe 2, outer aqueous phase pipe 3 and the geometry of emulsion collecting pipe 4 binding site, size and relative position, improve the relative velocity of the outer aqueous phase of binding site, and how to realize the connection of inside and outside pipeline.
Fig. 2 gives the Basic Design of the present invention, and main points are as follows:
(1) interior aqueous phase and oil phase pipeline take coaxial conical tube structure, it is simple to the formation of Water-In-Oil drop；Caliber tapers into, and reaches the aperture of regulation；
(2) outer aqueous phase and emulsion pipeline adopt reverse coaxial configuration, go out in entering outward；It is more and more less that outer aqueous phase flows to the position sectional area changed, and reaches accelerate fluid velocity and reduce the effect of pressure, is conducive to formation and the refinement of emulsion particle；
(3) end of outer aqueous phase/latex vessel adopts circular ring structure, in order to runner smooths, and flowing is stable；
(4) internal diameter of outer aqueous phase/latex vessel end circular loop and oil phase tube end size match, and the narrowest at annulus the narrowest place runner, and flow velocity is the fastest；
(5) all liq inlet and outlet adopts standardized designs, it is simple to the connection of outer pipeline and delivery pump.
From the partial enlarged drawing of Fig. 3, above characteristic sees more clearly.
Obviously, such complex precise is closed again structure injection moulding or the method for machining are difficult to realization.
Embodiment 1:
The present invention uses the technology of photosensitive resin ink jet printing/ultraviolet light polymerization to carry out overall processing, successively print from left to right, print thickness 0.02～0.1mm, by digital light treatment technology (DLP), with 405nm irradiation under ultraviolet ray shaping position, illumination curing time about 500～4000ms；It comprises the following steps:
(1) modeling of 3D mathematical model is completed according to plane design drawing；
(2) 3D digital-to-analogue is imported in print software, by parameters such as the distance of software set support intercolumniation and diameter, printing thickness, hardening times；
(3) print software is according to set printing thickness, treat print structure and carry out " section ", obtain the lamella of a series of uniform thickness, it is determined that the exact position of DLP projected light photograph, intensity of illumination and time in each lamella, and the action command of Z axis controlling organization and parameter；
(4) 3D printing is carried out；
(5) supporter, surface refine are removed.
Embodiment 1: one step completed microfluidic device
Being processed into microfluidic device as shown in Figures 2 and 3 with inkjet printing/UV curing method, design size is as follows: body outer diameter 20mm, wall thickness 3mm, length 36mm；Left side cone length 13.5mm, interior aqueous phase pipe cone angle 3 °, oil phase pipe outside cone angle 52 °；Aqueous phase bore 0.4mm in exit portion, wall thickness 0.2mm, oil phase pipe exit inside diameter 0.6mm, interior aqueous phase pipe is relative to oil phase pipe indentation 1mm；Right side pipe internal radius 1.0mm, annulus external diameter 7mm；All external inlet and outlet adopt Pagoda-shaped straight tube, internal diameter 3mm, join 6mm(16#) flexible pipe.
This 3D print it is crucial that the design of binding site supporting construction because being empty between the outlet of left side oil phase pipe and right side annular entrance, it does not have support and be impossible to print right side pipeline.The present invention adopts flake supporter, as shown in Figure 4,12 supporter circumferentially radial distribution thick for about 0.2mm, outer circumference diameter 7mm, inner circumferential diameter 4mm, just support the outer half cycle of annular entrance.These supporters are not removed after whole device has printed, and have substantially no effect on the flowing of outer aqueous phase.
In order to reduce inner support, outer aqueous phase inwall is designed to cylinder or circular cone, and by arc transition to root (Fig. 2).So, the printing of right side need not support.
Owing to being print from left to right, the print setting conventional support of left side.
Embodiment 2:
Although owing to above-mentioned interior aqueous phase and oil phase pipe are coaxial with outer aqueous phase/latex vessel, but not contacting, 3D prints still certain difficulty, therefore the second of the present invention embodiment is that two parts 3D respectively in left and right is printed processing, then connects with rib-loop/sealing ring, sees Fig. 5.The advantage of this scheme is that 3D print over is easier to, and left and right two parts print with right-to-left respectively from left to right layer by layer, it is not necessary to complicated inside temporary support, and outer aqueous phase pipe adopts straight tube structure.
Embodiment 2: two-piece-dress microfluidic device
It is processed into two, left and right parts as shown in Figure 5 with inkjet printing/UV curing method, then fits together.Wherein screw thread specification M22 × 1mm, O specification 15 × 2.65mm；Left part part external diameter 20mm, wall thickness 3mm, total length and cone length 34mm, interior aqueous phase pipe cone angle 5 °, oil phase pipe outside cone angle 20 °；Aqueous phase bore 0.2mm in exit portion, oil phase bore 0.65mm, two ligament 0.13mm；Right members external diameter ∮ 27mm, wall thickness 5.5mm；Pipe internal radius 0.6mm, annulus external diameter 6.6mm, annulus end face is to housing right side 18mm；All external inlet and outlet adopt Pagoda-shaped straight tube, internal diameter 3mm, join 6mm(16#) flexible pipe.
By design size, when M22 × 1mm is threaded onto, left part part right side is 0 with the spacing of pipe annulus end face in right members.On the ∮ 27mm excircle of left part part, first carve 50 scales, then carve index point on right members ∮ 27mm excircle, then rotate right members shell, so that it may adjust and calculate the relative position of two above end face.
Embodiment 3:
On this basis, we improve further, the interior aqueous phase of left half and oil phase pipe are exported, are not directly be printed as plane, but are printed as vertebral body completely, as shown in Figure 6, then truncated, expose interior aqueous phase pipe and the outlet of oil phase pipe.The advantage of this design is: with the same 3D blank printed, it is possible to obtain the product of different bore easily.Laboratory research is highly significant by this.The method cutting tip: (1) cuts, (2) polish, and (3) cut and polish.When aqueous phase pipe indentation in needs, the method that laser ablation can be adopted.The end that crop is later, it is also possible to refine further, as round and smooth in made corner angle.
Embodiment 3: left part part is printed as complete vertebral body
It is processed into left part part as shown in Figure 6 with inkjet printing/UV curing method.All sizes are with embodiment 2 and Fig. 5, but conical section is not frustum, but Full Cone, the cone length counted from left side is 36mm, and its top is the circular cone of 53 °, then is transitioned into the garden groove of R3.5, it is ensured that outer aqueous phase runner smooths and cross section constantly reduces.
As shown in Figure 6, this member center section highlights, resected and/or polish, and all can obtain the finished product of different bore.Then assembling with right members, keep left the scale on parts ∮ 27mm excircle and the index point on right members ∮ 27mm excircle, and adjustable also reads the spacing of parts joint portion, left and right.
Beneficial effect
The present invention adopts the 3D technology printed, processing and manufacturing microfluidic device, is used for preparing " the double; two emulsion of water/oil/water ", has the advantage that
1. make use of the 3D outstanding advantages printed, i.e. processing and manufacturing fine structure, the complicated object even closed without mould, it is achieved that with the processing of the microfluidic device that injection moulding and machining cannot complete.
2. in the design of microfluidic device, interior aqueous phase pipe and oil phase pipe adopt coaxial conical tube structure, outer aqueous phase pipe adopts reverse coaxial configuration with latex vessel, go out in entering outward, and flow section transitions smooth, meeting the formation condition of the double; two emulsion of W/O/W, and the thickness of the diameter of interior aqueous phase liquid stream, oil phase liquid stream can reach 0.2～0.1mm, the bore of emulsion collecting pipe is in 1.0～0.5mm scope.These size factors are all conducive to formation and the refinement of emulsion particle.
3. second and the 3rd embodiment of the present invention, all takes first 3D to print left and right parts then the method for screw thread/sealing ring assembling.It reduce the disposable 3D of the whole device difficulty printed, it is simple to 3D repairs fine structure and the relative position of emulsion forming part further after printing, improve the combination property of whole device.
4. the size of inner flow passage of the present invention adopts standardized pagoda-shaped pipe and 16# flexible pipe in submillimeter magnitude, input and output.This is easy to the linking with other device of this device, is also that etching method microfluidic device is difficult to accomplish.
Accompanying drawing explanation
Fig. 1: microfluidic device schematic diagram.
Fig. 2: microfluidic device Basic Design.
Fig. 3: emulsion forming part fine structure.
Fig. 4: supporting construction schematic diagram (in the middle of Fig. 2 or Fig. 3, binding site section eyes right) between oil phase outer tube wall and emulsion collecting pipe annulus.
Fig. 5: the microfluidic device of two parts combination.
Fig. 6: first print cone, then cuts tip, exposes tube wall.
Claims (6)
1. the microfluidic device being suitable for preparing " W/O/W (W/O/W) " double, two emulsions continuously, as shown in Figure 2, this device is by interior aqueous phase pipe 1, oil phase pipe 2, outer aqueous phase pipe 3 is constituted with emulsion collecting pipe 4, interior aqueous phase pipe and oil phase pipe adopt coaxial conical tube structure, outer aqueous phase pipe adopts reverse coaxial configuration with latex vessel, go out in entering outward, flow section is transitions smooth from big to small, meet the formation condition of " the double, two emulsion of W/O/W ", the diameter of interior aqueous phase liquid stream, the thickness of oil phase liquid stream can reach 0.2-0.1mm, the inlet calibre of emulsion collecting pipe is in 1.0-0.5mm scope, device is connected by standardized hose coupling with extraneous.
2. the 3D of microfluidic device described in claim 1 prints manufacture method, even if successively printing and ultraviolet light polymerization with photosensitive ink resins water, print thickness 0.02-0.1mm, by digital light treatment technology (DLP), shaping position, illumination curing time 500-4000ms is successively irradiated with 405nm ultraviolet light；It comprises the following steps:
(1) modeling of 3D mathematical model is completed according to plane design drawing；
(2) 3D digital-to-analogue is imported in print software, by parameters such as the distance of software set support intercolumniation and diameter, printing thickness, hardening times；
(3) print software is according to set printing thickness, treat print structure and carry out " section ", obtain the lamella of a series of uniform thickness, it is determined that the exact position of DLP projected light photograph, intensity of illumination and time in each lamella, and the action command of Z axis controlling organization and parameter；
(4) 3D printing is carried out；
(5) supporter, surface refine are removed.
3. the Basic Design of microfluidic device described in claim 1, as shown in Figures 2 and 3, including following main points:
Interior aqueous phase and oil phase pipeline take coaxial conical tube structure, it is simple to the formation of Water-In-Oil drop；Caliber tapers into, and reaches the aperture of regulation；
Outer aqueous phase and emulsion pipeline adopt reverse coaxial configuration, go out in entering outward；It is more and more less that outer aqueous phase flows to the position sectional area changed, and reaches accelerate fluid velocity and reduce the effect of pressure, is conducive to the formation of emulsion particle and reduces emulsion droplet diameter；
The end of outer aqueous phase/latex vessel adopts circular ring structure, in order to runner smooths, and flowing is stable；
The internal diameter of outer aqueous phase/latex vessel end circular loop and oil phase tube end size match, and the narrowest at annulus the narrowest place runner, and flow velocity is the fastest；
All liq inlet and outlet adopts standardized designs, it is simple to be connected with outer pipeline and delivery pump.
4. the 3D Method of printing of microfluidic device as claimed in claim 1, is characterized in that resolving into whole device left and right two parts, and 3D prints processing respectively, then with rib-loop/sealing ring connection, sees Fig. 5.
5. the 3D Method of printing of microfluidic device as claimed in claim 4, it is characterized in that left half tubular outlet portion is designed to complete cone, carry out 3D and print processing, then adopt cutting, the method for polishing cuts cone tip, expose pipe outlet, use the method for laser ablation by interior aqueous phase pipe indentation 0.5-1mm as required, be finally assembled into microfluidic device by rib-loop/sealing ring method of attachment and right members.
6. the 3D Method of printing of microfluidic device as described in claim 4,5, is characterized in that periphery, two-part threaded connection place carves or draw reticule and index point in left and right, in order to adjust and calculate microfluidic device emulsion forming part between left and right from.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410747264.3A CN105727857A (en) | 2014-12-10 | 2014-12-10 | Microfluidic apparatus produced by 3D printing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410747264.3A CN105727857A (en) | 2014-12-10 | 2014-12-10 | Microfluidic apparatus produced by 3D printing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105727857A true CN105727857A (en) | 2016-07-06 |
Family
ID=56237170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410747264.3A Pending CN105727857A (en) | 2014-12-10 | 2014-12-10 | Microfluidic apparatus produced by 3D printing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105727857A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106179148A (en) * | 2016-07-21 | 2016-12-07 | 昆明理工大学 | The microreactor of a kind of accurate amplification, preparation method and applications |
| CN106311367A (en) * | 2016-09-29 | 2017-01-11 | 东南大学 | Firm-core-containing duplex-emulsion preparation micro fluid control device and preparation method thereof |
| CN106622407A (en) * | 2016-09-29 | 2017-05-10 | 东南大学 | Capillary microfluidic control device for solid core package, and preparation method thereof |
| CN106975411A (en) * | 2017-05-05 | 2017-07-25 | 北京大学 | Micro-fluidic chip based on 3D printing and the emulsion-producing device including the chip |
| CN108159976A (en) * | 2018-01-03 | 2018-06-15 | 西南交通大学 | A kind of Water-In-Oil Bao Shui（W/W/O）Monodisperse double emulsion preparation method and its micro fluidic device |
| CN110694704A (en) * | 2019-09-26 | 2020-01-17 | 安徽省昂普拓迈生物科技有限责任公司 | Portable quick micro-droplet generator |
| CN114246223A (en) * | 2021-12-13 | 2022-03-29 | 江南大学 | Preparation of vegetable protein-based W/O/W lipid and 3D printing application |
| CN114586874A (en) * | 2022-03-10 | 2022-06-07 | 江南大学 | Method for realizing multi-structure low-fat chocolate two-channel 4D printing by inducing deformation |
| CN114887562A (en) * | 2022-05-17 | 2022-08-12 | 成都科建生物医药有限公司 | Method for preparing liposome |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008168175A (en) * | 2007-01-09 | 2008-07-24 | National Agriculture & Food Research Organization | Method and device for manufacturing shelled micro-bubble |
| CN102014871A (en) * | 2007-03-28 | 2011-04-13 | 哈佛大学 | Emulsions and techniques for formation |
| CN103285950A (en) * | 2013-05-27 | 2013-09-11 | 苏州扬清芯片科技有限公司 | Preparation method for polymer micro-fluidic chip |
| CN103895226A (en) * | 2014-03-24 | 2014-07-02 | 浙江大学 | 3D-printing-based machining method of three-dimensional micro-fluidic chip and printing device |
-
2014
- 2014-12-10 CN CN201410747264.3A patent/CN105727857A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008168175A (en) * | 2007-01-09 | 2008-07-24 | National Agriculture & Food Research Organization | Method and device for manufacturing shelled micro-bubble |
| CN102014871A (en) * | 2007-03-28 | 2011-04-13 | 哈佛大学 | Emulsions and techniques for formation |
| CN103285950A (en) * | 2013-05-27 | 2013-09-11 | 苏州扬清芯片科技有限公司 | Preparation method for polymer micro-fluidic chip |
| CN103895226A (en) * | 2014-03-24 | 2014-07-02 | 浙江大学 | 3D-printing-based machining method of three-dimensional micro-fluidic chip and printing device |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106179148B (en) * | 2016-07-21 | 2018-10-02 | 昆明理工大学 | A kind of microreactor accurately amplified, preparation method and applications |
| CN106179148A (en) * | 2016-07-21 | 2016-12-07 | 昆明理工大学 | The microreactor of a kind of accurate amplification, preparation method and applications |
| CN106311367A (en) * | 2016-09-29 | 2017-01-11 | 东南大学 | Firm-core-containing duplex-emulsion preparation micro fluid control device and preparation method thereof |
| CN106622407A (en) * | 2016-09-29 | 2017-05-10 | 东南大学 | Capillary microfluidic control device for solid core package, and preparation method thereof |
| CN106975411B (en) * | 2017-05-05 | 2020-01-10 | 北京大学 | Micro-fluidic chip based on 3D prints and including emulsion generating device of this chip |
| CN106975411A (en) * | 2017-05-05 | 2017-07-25 | 北京大学 | Micro-fluidic chip based on 3D printing and the emulsion-producing device including the chip |
| CN108159976A (en) * | 2018-01-03 | 2018-06-15 | 西南交通大学 | A kind of Water-In-Oil Bao Shui（W/W/O）Monodisperse double emulsion preparation method and its micro fluidic device |
| CN110694704A (en) * | 2019-09-26 | 2020-01-17 | 安徽省昂普拓迈生物科技有限责任公司 | Portable quick micro-droplet generator |
| CN110694704B (en) * | 2019-09-26 | 2021-10-12 | 安徽省昂普拓迈生物科技有限责任公司 | Portable quick micro-droplet generator |
| CN114246223A (en) * | 2021-12-13 | 2022-03-29 | 江南大学 | Preparation of vegetable protein-based W/O/W lipid and 3D printing application |
| CN114586874A (en) * | 2022-03-10 | 2022-06-07 | 江南大学 | Method for realizing multi-structure low-fat chocolate two-channel 4D printing by inducing deformation |
| CN114887562A (en) * | 2022-05-17 | 2022-08-12 | 成都科建生物医药有限公司 | Method for preparing liposome |
| CN114887562B (en) * | 2022-05-17 | 2024-03-12 | 成都科建生物医药有限公司 | Preparation method of liposome |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105727857A (en) | Microfluidic apparatus produced by 3D printing | |
| Scott et al. | Fabrication methods for microfluidic devices: An overview | |
| Bandulasena et al. | Versatile reconfigurable glass capillary microfluidic devices with Lego® inspired blocks for drop generation and micromixing | |
| Catarino et al. | Blood cells separation and sorting techniques of passive microfluidic devices: From fabrication to applications | |
| US11360057B2 (en) | Fluid distributor unit | |
| Chen et al. | Millifluidics, microfluidics, and nanofluidics: manipulating fluids at varying length scales | |
| CN108289797B (en) | Systems and methods for making and using gel microspheres | |
| Serex et al. | Microfluidics: A new layer of control for extrusion-based 3D printing | |
| US8563325B1 (en) | Coaxial microreactor for particle synthesis | |
| De La Vega et al. | Uniform polymer microspheres: monodispersity criteria, methods of formation and applications | |
| Gao et al. | Self-ordered particle trains in inertial microchannel flows | |
| JP7041878B2 (en) | General purpose, flexible and biocompatible elastomer microtube | |
| Lao et al. | Tethered and untethered 3D microactuators fabricated by two-photon polymerization: a review | |
| Zhang et al. | Droplet generation in cross-flow for cost-effective 3D-printed “plug-and-play” microfluidic devices | |
| CN106669556B (en) | A method of millimeter grade particles are prepared using transformation microfluidic channel | |
| CN106622407A (en) | Capillary microfluidic control device for solid core package, and preparation method thereof | |
| Li et al. | Perturbation-induced droplets for manipulating droplet structure and configuration in microfluidics | |
| Lim et al. | Analysis of liquid–liquid droplets fission and encapsulation in single/two layer microfluidic devices fabricated by xurographic method | |
| CN105921188A (en) | Microfluidic device capable of rapidly preparing emulsion in multiple dimensions | |
| Ye et al. | 3D-printed air-blast microfluidic nozzles for preparing calcium alginate microparticles | |
| CN112892630A (en) | Multi-material liquid drop generation system based on coaxial flow focusing structure | |
| Chen et al. | Microfluidic generation of high-viscosity droplets by surface-controlled breakup of segment flow | |
| Zhang et al. | From core-shell to Janus: microfluidic preparation and morphology transition of Gas/Oil/Water emulsions | |
| WO2020104786A1 (en) | Modular microfluidic device for mirco-mixing fluids | |
| Xia et al. | Recent progress in preparation of functional microparticles based on microfluidic technique |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160706 |
|
| WD01 | Invention patent application deemed withdrawn after publication |