CN105724247A - A micro-cutting rapid breeding method for castanopsis hystrix seedlings - Google Patents
A micro-cutting rapid breeding method for castanopsis hystrix seedlings Download PDFInfo
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- CN105724247A CN105724247A CN201410756961.5A CN201410756961A CN105724247A CN 105724247 A CN105724247 A CN 105724247A CN 201410756961 A CN201410756961 A CN 201410756961A CN 105724247 A CN105724247 A CN 105724247A
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Abstract
A micro-cutting rapid breeding method for castanopsis hystrix seedlings is disclosed. The method includes inoculating a castanopsis hystrix seed to a germination culture medium, culturing to obtain a seedling, cutting the seedling into stem segments, inserting the stem segments into a micro-cutting culture medium, culturing until lateral buds occur, cutting the lateral buds into stem segments, inserting the stem segments into a fresh micro-cutting culture medium, culturing, cutting the obtained lateral buds into stem segments, inserting the stem segments into a rooting culture medium, and culturing until the stem segments root, thus preparing the castanopsis hystrix seedlings. The method is simple and feasible in process and high in breeding speed, overcomes the problem that castanopsis hystrix seeds are difficult to collect due to biennial bearing, achieves long-term large-scale rapid breeding of the castanopsis hystrix seedlings, can prepare high-quality castanopsis hystrix test-tube plantlets in a large-scale, rapid and continuous manner, and is high in practicality.
Description
Technical field
The invention belongs to agricultural biological technical field, a kind of method being specially stem red cone seedling of section micro-cuttage Fast-propagation.
Background technology
Red cone (Castanopsishystrix) is Fagaceae Castanopsis aiphyllium, all there is distribution south, the Motuo County in Tibet that Guangxi, Guangdong, Hainan, Yunnan, Guizhou and Hunan, Jiangxi, Fujian etc. are economized, and central distribution is in the massif area of south Guangxi below height above sea level 700m.Its growth is very fast, and rudiment power is strong, is thick with leaves, more resistance to concealment, and mixed natural disposition can be good, selects seeds on knob, south China and pine, China fir or other broad leaf tree Mixed forestation.Red cone material is excellent, the hard corrosion resistant of timber, and heartwood is big, maroon, sapwood pale red, color and luster and beautiful texture, few deformation, dried cracking is little, tangent plane is smooth, and gumminess and paint are functional, are the high quality timber such as noble furniture, shipbuilding, vehicle, technique engraving, construction and decoration；Branch, flaw-piece, broken material, wood shavings etc. are the good materials of staple fibre, paper pulp, fibre board, particieboard etc., are also the quality raw materials cultivating edible fungi；Bark and acorn-cup, containing tannin 10～15%, can make tannic extract raw material, are the excellent precious indigenous trees of Guangxi current spread afforestation.
Current red cone propagation method is mainly seminal propagation, but the red obvious biennial bearing phenomenon of the solid existence of cone nut tree, solid little older length because can not meet afforestation needs less than seed.
In recent years, cottage propagation and tissue culture propagation become the research emphasis of red cone sapling multiplication, and cottage propagation survival rate is low；Tissue culture propagation adopts the mode of callus induction, Calli Differentiation propagation and adventitious bud rooting to obtain aseptic seedling at present, and this mode there is the problem that (1) callus induction process time is longer；(2) Calli Differentiation is that the frequency of adventitious bud is relatively low；(3) the Elongation of adventitious bud speed that Calli Differentiation goes out is slow, and the time reaching the standard of taking root is long.These problems seriously hinder the large-scale production of red cone seedling, thus cannot meet the market demand to red cone.For that purpose it is necessary to foundation energy is a large amount of, the production technology system of the red cone seedling of fast-propagation.
Summary of the invention
The method that it is an object of the invention to provide the red cone seedling of a kind of micro-cuttage Fast-propagation is a kind of stem section sprouting of lateral bud and extends fireballing feature, it is achieved that the purpose of red cone seedling vast propagation.
The present invention utilizes a small amount of seed to obtain aseptic seedling, with aseptic seedling stem sections for outer implant, promotes that lateral bud is constantly sprouted by test tube micro-cuttage and carries out fast breeding, it is achieved red cone seedling vast propagation.
The technical solution adopted in the present invention: the method for the red cone seedling of a kind of micro-cuttage Fast-propagation, including the acquisition process of aseptic seedling, stem section micro-cuttage process, stem section rooting process.
1, the acquisition process of aseptic seedling
Choose the nut of red cone sterilized after seed is stripped out, seed is inoculated into germination culture medium and carries out being cultured to and grow up to seedling, described germination culture medium is with MS culture medium for minimal medium additional 6-benzyladenine, α-naphthaleneacetic acid.
2, stem section micro-cuttage process
Seedling step 1 obtained is cut into stem section, is downwardly in micro-cuttage culture medium by the base portion of stem section to carry out being cultured to and grows lateral bud, then lateral bud is cut into stem section, is then downwardly in fresh micro-cuttage culture medium by its base portion and cultivates.Described micro-cuttage culture medium is with MS culture medium for minimal medium additional 6-benzyladenine, heteroauxing.
3, stem section rooting process
Lateral bud step 2 obtained is cut into stem section, and is inoculated on root media, and first light culture carries out illumination cultivation again, and taking root to stem section to obtain red cone seedling.Described root media is with 1/2MS culture medium for minimal medium additional IAA, IBA.
Present invention process is simple, and growth rate is fast, overcomes red cone seed and collects seed difficult problem because of biennial bearing phenomenon, it is achieved that the long-term extensive fast breeding of red cone seedling, can in a large number, quickly, continue to obtain high-quality red cone test tube Seedling, practical.Provide efficient method for red cone vast propagation propagation, lay a good foundation for the scale of red cone seedling, factorial praluction.
Detailed description of the invention
The invention will be further described below.
The method of the red cone seedling of a kind of micro-cuttage Fast-propagation provided by the present invention, including the acquisition process of aseptic seedling, stem section micro-cuttage process, stem section rooting process.
1), the acquisition process of aseptic seedling
Choose the nut of red cone sterilized after seed is stripped out, seed is inoculated into germination culture medium and carries out being cultured to and grow up to seedling, cultivation temperature degree is 25～28 DEG C, and intensity of illumination is 15～20 μm of ol m-2·s-1, light application time is 10h d-1, cultivate 30～40 days；Described germination culture medium is with MS culture medium for minimal medium additional 6-benzyladenine 0.2mg L-1, α-naphthaleneacetic acid 0.05mg L-1。
2), stem section micro-cuttage process
Seedling step 1 obtained is cut into the stem section with 1～2 joint, it is downwardly in micro-cuttage culture medium by the base portion of stem section to carry out being cultured to growing lateral bud, lateral bud is cut into the stem section with 1～2 joint again, then its base portion is downwardly in fresh micro-cuttage culture medium and cultivates, cultivation temperature degree is 25～28 DEG C, and intensity of illumination is 30～40 μm of ol m-2·s-1, light application time is 10h d-1；Described micro-cuttage culture medium is with MS culture medium for minimal medium additional 6-benzyladenine 2.0mg L-1, heteroauxing 0.1mg L-1。
3), stem section rooting process
Lateral bud step 2 obtained is cut into the stem section with 1～2 joint, and is inoculated on root media, and first light culture carries out illumination cultivation 20～30 days in 3～5 days again, and taking root to stem section to obtain red cone seedling.Described root media is with 1/2MS culture medium for minimal medium additional IAA0.5mg L-1、IBA2.0mg·L-1。
Claims (1)
1. the method for the red cone seedling of micro-cuttage Fast-propagation, it is characterised in that: include the acquisition process of aseptic seedling, stem section micro-cuttage process, stem section rooting process；
1), the acquisition process of aseptic seedling
Choose the nut of red cone sterilized after seed is stripped out, seed is inoculated into germination culture medium and carries out being cultured to and grow up to seedling, cultivation temperature is 25～28 DEG C, and intensity of illumination is 15～20 μm of ol m-2·s-1, light application time is 10h d-1, cultivate 30～40 days；Described germination culture medium is with MS culture medium for minimal medium additional 6-benzyladenine 0.2mg L-1, α-naphthaleneacetic acid 0.05mg L-1；
2), stem section micro-cuttage process
Seedling step 1 obtained is cut into the stem section with 1～2 joint, it is downwardly in micro-cuttage culture medium by the base portion of stem section to carry out being cultured to growing lateral bud, lateral bud is cut into the stem section with 1～2 joint again, then its base portion is downwardly in fresh micro-cuttage culture medium and cultivates, cultivation temperature is 25～28 DEG C, and intensity of illumination is 30～40 μm of ol m-2·s-1, light application time is 10h d-1；Described micro-cuttage culture medium is with MS culture medium for minimal medium additional 6-benzyladenine 2.0mg L-1, heteroauxing 0.1mg L-1；
3), stem section rooting process
Lateral bud step 2 obtained is cut into the stem section with 1～2 joint, and is inoculated on root media, and first light culture carries out illumination cultivation 20～30 days in 3～5 days again, and taking root to stem section to obtain red cone seedling；Described root media is with 1/2MS culture medium for minimal medium additional IAA0.5mg L-1, IBA2mg L-1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410756961.5A CN105724247A (en) | 2014-12-11 | 2014-12-11 | A micro-cutting rapid breeding method for castanopsis hystrix seedlings |
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| CN201410756961.5A CN105724247A (en) | 2014-12-11 | 2014-12-11 | A micro-cutting rapid breeding method for castanopsis hystrix seedlings |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106879463A (en) * | 2017-02-14 | 2017-06-23 | 唐春艳 | Red cone aseptic explant is prepared and initial bud abductive approach |
| CN115380822A (en) * | 2022-08-03 | 2022-11-25 | 中国林业科学研究院高原林业研究所 | High mountain tannin extract tissue culture medium and tissue culture method |
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2014
- 2014-12-11 CN CN201410756961.5A patent/CN105724247A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106879463A (en) * | 2017-02-14 | 2017-06-23 | 唐春艳 | Red cone aseptic explant is prepared and initial bud abductive approach |
| CN115380822A (en) * | 2022-08-03 | 2022-11-25 | 中国林业科学研究院高原林业研究所 | High mountain tannin extract tissue culture medium and tissue culture method |
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Application publication date: 20160706 |
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