CN105706566A - Method for collecting seeds bred by improved tomato variety - Google Patents
Method for collecting seeds bred by improved tomato variety Download PDFInfo
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- CN105706566A CN105706566A CN201610087009.XA CN201610087009A CN105706566A CN 105706566 A CN105706566 A CN 105706566A CN 201610087009 A CN201610087009 A CN 201610087009A CN 105706566 A CN105706566 A CN 105706566A
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- seed
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- fructus lycopersici
- lycopersici esculenti
- fermentation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention relates to the technical field of a tomato seed collecting method, and provides a method for collecting seeds bred by an improved tomato variety. The method for collecting the seeds bred by the improved tomato variety comprises the following steps that 1, tomato fruit obtained by planting the improved tomato variety is subjected to beating, and a tomato seed slurry is obtained; 2, pectinase is added to the tomato seed slurry, the tomato seed slurry and the pectinase are mixed to be uniform, and a seed slurry to be fermented is obtained. According to the method for collecting the seeds bred by the improved tomato variety, the fermentation time is short compared with that of an existing method for collecting the seeds bred by an improved tomato variety, and therefore the time for collecting the seeds bred by the improved tomato variety is shortened; meanwhile, the risk that the seed germination rate is decreased due to the fact that the fermentation time is long is reduced, the influence, caused by the long fermentation time, on the quality of the collected seeds is eliminated, and the seed using safety of farmers is guaranteed.
Description
Technical field
The present invention relates to the Seed Collecting Methods technical field of tomato seeds, be the Seed Collecting Methods of a kind of Fructus Lycopersici esculenti stock breeding seed.
Background technology
Develop agriculture through science and technology, breeding is leading, agricultural is not had without seed, seed quality and technology quality directly influence the safety of whole industry, the quality of seed quality directly affects yield and the quality of crops, adding up according to the Ministry of Agriculture, the contribution rate of increases in grain production is brought up to 40% in 2009 by breeding by 36% in 2003.Therefore, the maintenance of Fructus Lycopersici esculenti stock breeding seed is significant.At present, when breeding Fructus Lycopersici esculenti is collected seed by the Seed Collecting Methods adopting existing Fructus Lycopersici esculenti stock breeding seed, its fermentation time was up to 24 hours to 28 hours, owing to its fermentation time is longer, the seed collecting time not only making Fructus Lycopersici esculenti stock breeding seed is longer, and there is the risk that seed bud rate reduces, affect seed quality.
Summary of the invention
The invention provides the Seed Collecting Methods of a kind of Fructus Lycopersici esculenti stock breeding seed, overcome the deficiency of above-mentioned prior art, it can effectively solve there is, in the existing seed collecting process sub in the good breeding of Fructus Lycopersici esculenti, the problem that fermentation time is longer.
The technical scheme is that and realized by following measures: the Seed Collecting Methods of a kind of Fructus Lycopersici esculenti stock breeding seed, carry out in the steps below: the first step, after the tamato fruit making beating plantation of Fructus Lycopersici esculenti breeding obtained, obtain Tomato Seed slurry;Second step, pectase is added to Tomato Seed in starching, obtain after being mixed homogeneously with pectase by Tomato Seed slurry treating fermentation seed slurry, feed liquid will be obtained after fermentation seed slurry ferments under air-proof condition, wherein, the Tomato Seed slurry of every double centner adds the pectase of 15 milliliters to 17 milliliters, the temperature of fermentation is 20 DEG C to 28 DEG C, and the time of fermentation is 4 hours to 6 hours, in the process of fermentation, stirred once every 1 hour to 2 hour, and avoid water to enter in the fermentation seed slurry being in yeastiness;3rd step, through rinsing, immersion and obtains Fructus Lycopersici esculenti stock breeding seed by feed liquid after drying.
Further optimization and/or improvements to foregoing invention technical scheme are presented herein below:
In above-mentioned 3rd step, feed liquid sequentially passes through rinsing for the first time, first time soaks, rinse for the second time, second time is soaked, third time rinses and obtains Fructus Lycopersici esculenti stock breeding seed after drying, wherein, first time rinsing, second time rinsing and third time rinsing all adopt clear water to rinse, the time of second time rinsing and the time of third time rinsing are 15 minutes to 20 minutes, the soak that first time immersion adopts is mass percent is the trisodium phosphate aqueous solution of 10% to 12%, and the soak that second time immersion adopts is mass percent is the aqueous hydrochloric acid solution of 1% to 1.5%.
The time that above-mentioned first time soaks is 20 minutes to 23 minutes;Or/and, the time that second time is soaked is 30 minutes to 32 minutes.
The above-mentioned tamato fruit of plantation in Fructus Lycopersici esculenti seed farm of gathering, removes the disease pest fruit in Fructus Lycopersici esculenti seed farm, fallen fruits, decayed fruit and hybrid strain.
In above-mentioned dry run, by seed dehydration 3 minutes to 5 minutes in dewaterer after rinsing and immersion, then, seed is placed on yarn bed and spreads out, and by seed dry in the sun under the sun, after dry in the sun is dry, obtain Fructus Lycopersici esculenti stock breeding seed.
The fermentation time of the Seed Collecting Methods of Fructus Lycopersici esculenti stock breeding seed of the present invention is for the fermentation time of the Seed Collecting Methods of existing Fructus Lycopersici esculenti stock breeding seed, the fermentation time of the Seed Collecting Methods of Fructus Lycopersici esculenti stock breeding seed of the present invention is shorter, thus shortening the seed collecting time of Fructus Lycopersici esculenti stock breeding seed, simultaneously, reduce the risk that the seed bud rate caused due to fermentation time length reduces, thus eliminating the impact owing to the seed quality gathered is caused by fermentation time length, it is ensured that peasant household's kind of safety.
Detailed description of the invention
The present invention, not by the restriction of following embodiment, can determine specific embodiment according to technical scheme and practical situation.
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1: the Seed Collecting Methods of this Fructus Lycopersici esculenti stock breeding seed, carries out: the first step in the steps below, obtains Tomato Seed slurry after the tamato fruit making beating plantation of Fructus Lycopersici esculenti breeding obtained;Second step, pectase is added to Tomato Seed in starching, obtain after being mixed homogeneously with pectase by Tomato Seed slurry treating fermentation seed slurry, feed liquid will be obtained after fermentation seed slurry ferments under air-proof condition, wherein, the Tomato Seed slurry of every double centner adds the pectase of 15 milliliters to 17 milliliters, the temperature of fermentation is 20 DEG C to 28 DEG C, and the time of fermentation is 4 hours to 6 hours, in the process of fermentation, stirred once every 1 hour to 2 hour, and avoid water to enter in the fermentation seed slurry being in yeastiness;3rd step, through rinsing, immersion and obtains Fructus Lycopersici esculenti stock breeding seed by feed liquid after drying.When the Seed Collecting Methods adopting the Fructus Lycopersici esculenti stock breeding seed described in the present embodiment is collected seed, the fermentation time of the Seed Collecting Methods of the Fructus Lycopersici esculenti stock breeding seed described in the present embodiment is 4 hours to 6 hours, for the fermentation time (24 hours to 28 hours) of the Seed Collecting Methods of existing Fructus Lycopersici esculenti stock breeding seed, the fermentation time of the Seed Collecting Methods of the Fructus Lycopersici esculenti stock breeding seed described in the present embodiment is shorter, thus shortening the seed collecting time of Fructus Lycopersici esculenti stock breeding seed, simultaneously, reduce the risk causing bud rate to reduce due to fermentation time length, thus eliminating the impact owing to the seed quality gathered is caused by fermentation time length, ensure peasant household's kind of safety.
Embodiment 2: the Seed Collecting Methods of this Fructus Lycopersici esculenti stock breeding seed, carries out: the first step in the steps below, obtains Tomato Seed slurry after the tamato fruit making beating plantation of Fructus Lycopersici esculenti breeding obtained;Second step, pectase is added to Tomato Seed in starching, obtain after being mixed homogeneously with pectase by Tomato Seed slurry treating fermentation seed slurry, feed liquid will be obtained after fermentation seed slurry ferments under air-proof condition, wherein, the Tomato Seed slurry of every double centner adds the pectase of 15 milliliters or 17 milliliters, the temperature of fermentation is 20 DEG C or 28 DEG C, and the time of fermentation is 4 hours or 6 hours, in the process of fermentation, stirred once every 1 hour or 2 hours, and avoid water to enter in the fermentation seed slurry being in yeastiness;3rd step, through rinsing, immersion and obtains Fructus Lycopersici esculenti stock breeding seed by feed liquid after drying.
Embodiment 3: the Seed Collecting Methods of this Fructus Lycopersici esculenti stock breeding seed, carries out: the first step in the steps below, obtains Tomato Seed slurry after the tamato fruit making beating plantation of Fructus Lycopersici esculenti breeding obtained;Second step, pectase is added to Tomato Seed in starching, obtain after being mixed homogeneously with pectase by Tomato Seed slurry treating fermentation seed slurry, feed liquid will be obtained after fermentation seed slurry ferments under air-proof condition, wherein, the Tomato Seed slurry of every double centner adds 15 milliliters of pectases, the temperature of fermentation is 28 DEG C, and the time of fermentation is 4 hours, in the process of fermentation, stirred once every 1 hour, and avoid water to enter in the fermentation seed slurry being in yeastiness;3rd step, through rinsing, immersion and obtains Fructus Lycopersici esculenti stock breeding seed by feed liquid after drying.When the Seed Collecting Methods adopting the Fructus Lycopersici esculenti stock breeding seed described in the present embodiment is collected seed, the fermentation time of the Seed Collecting Methods of the Fructus Lycopersici esculenti stock breeding seed described in the present embodiment is 4 hours, for the fermentation time (24 hours to 28 hours) of the Seed Collecting Methods of existing Fructus Lycopersici esculenti stock breeding seed, the fermentation time of the Seed Collecting Methods of the Fructus Lycopersici esculenti stock breeding seed described in the present embodiment is shorter, thus shortening the seed collecting time of Fructus Lycopersici esculenti stock breeding seed, simultaneously, reduce the risk that seed causes bud rate to reduce due to fermentation time length, thus eliminating the impact owing to the seed quality gathered is caused by fermentation time length, ensure peasant household's kind of safety.
Embodiment 4: the Seed Collecting Methods of this Fructus Lycopersici esculenti stock breeding seed, carries out: the first step in the steps below, obtains Tomato Seed slurry after the tamato fruit making beating plantation of Fructus Lycopersici esculenti breeding obtained;Second step, pectase is added to Tomato Seed in starching, obtain after being mixed homogeneously with pectase by Tomato Seed slurry treating fermentation seed slurry, feed liquid will be obtained after fermentation seed slurry ferments under air-proof condition, wherein, the Tomato Seed slurry of every double centner adds the pectase of 16.5 milliliters, the temperature of fermentation is 21 DEG C, and the time of fermentation is 6 hours, in the process of fermentation, stirred once every 2 hours, and avoid water to enter in the fermentation seed slurry being in yeastiness;3rd step, through rinsing, immersion and obtains Fructus Lycopersici esculenti stock breeding seed by feed liquid after drying.When the Seed Collecting Methods adopting the Fructus Lycopersici esculenti stock breeding seed described in the present embodiment is collected seed, the fermentation time of the Seed Collecting Methods of the Fructus Lycopersici esculenti stock breeding seed described in the present embodiment is 6 hours, for the fermentation time (24 hours to 28 hours) of the Seed Collecting Methods of existing Fructus Lycopersici esculenti stock breeding seed, the fermentation time of the Seed Collecting Methods of the Fructus Lycopersici esculenti stock breeding seed described in the present embodiment is shorter, thus shortening the seed collecting time of Fructus Lycopersici esculenti stock breeding seed, simultaneously, reduce seed and there is the risk that bud rate reduces due to fermentation time length, thus eliminating the harmful effect owing to the seed quality gathered is caused by fermentation time length, ensure peasant household's kind of safety.
Embodiment 5: as the optimization of above-described embodiment, in 3rd step, feed liquid is sequentially through first time rinsing, first time soaks, second time rinsing, second time is soaked, third time rinses and obtains Fructus Lycopersici esculenti stock breeding seed after drying, wherein, first time rinsing, second time rinsing and third time rinsing all adopt clear water to rinse, the time of second time rinsing and the time of third time rinsing are 15 minutes to 20 minutes, the soak that first time immersion adopts is mass percent is the trisodium phosphate aqueous solution of 10% to 12%, the soak that second time immersion adopts is mass percent is the aqueous hydrochloric acid solution of 1% to 1.5%.
Embodiment 6: as the optimization of above-described embodiment, the time that first time soaks is 20 minutes to 23 minutes;Or/and, the time that second time is soaked is 30 minutes to 32 minutes.
Embodiment 7: as the optimization of above-described embodiment, the tamato fruit of plantation in Fructus Lycopersici esculenti seed farm of gathering, remove the disease pest fruit in Fructus Lycopersici esculenti seed farm, fallen fruits, decayed fruit and hybrid strain.By the disease pest fruit in removing Fructus Lycopersici esculenti, fallen fruits, decayed fruit and hybrid strain, it is possible to ensure the purity Coriolis mass of Fructus Lycopersici esculenti stock breeding seed.
Embodiment 8: as the optimization of above-described embodiment, in dry run, by seed dehydration 3 minutes to 5 minutes in dewaterer after rinsing and immersion, then, it is placed on seed on yarn bed to spread out, and by seed dry in the sun under the sun, after dry in the sun is dry, obtains Fructus Lycopersici esculenti stock breeding seed.
In sum, the fermentation time of the Seed Collecting Methods of Fructus Lycopersici esculenti stock breeding seed of the present invention is for the fermentation time of the Seed Collecting Methods of existing Fructus Lycopersici esculenti stock breeding seed, the fermentation time of the Seed Collecting Methods of Fructus Lycopersici esculenti stock breeding seed of the present invention is shorter, thus shortening the seed collecting time of Fructus Lycopersici esculenti stock breeding seed, simultaneously, reduce the risk that seed causes bud rate to reduce due to fermentation time length, thus eliminating the impact owing to the seed quality gathered is caused by fermentation time length, it is ensured that peasant household's kind of safety.
Above technical characteristic constitutes embodiments of the invention, and it has stronger adaptability and implementation result, can increase and decrease non-essential technical characteristic according to actual needs, meet the demand of different situations.
Claims (6)
1. the Seed Collecting Methods of a Fructus Lycopersici esculenti stock breeding seed, it is characterised in that carry out in the steps below: the first step, obtains Tomato Seed slurry after the tamato fruit making beating plantation of Fructus Lycopersici esculenti breeding obtained;Second step, pectase is added to Tomato Seed in starching, obtain after being mixed homogeneously with pectase by Tomato Seed slurry treating fermentation seed slurry, feed liquid will be obtained after fermentation seed slurry ferments under air-proof condition, wherein, the Tomato Seed slurry of every double centner adds the pectase of 15 milliliters to 17 milliliters, the temperature of fermentation is 20 DEG C to 28 DEG C, and the time of fermentation is 4 hours to 6 hours, in the process of fermentation, stirred once every 1 hour to 2 hour, and avoid water to enter in the fermentation seed slurry being in yeastiness;3rd step, through rinsing, immersion and obtains Fructus Lycopersici esculenti stock breeding seed by feed liquid after drying.
2. the Seed Collecting Methods of Fructus Lycopersici esculenti stock breeding seed according to claim 1, it is characterized in that in the 3rd step, feed liquid is sequentially through first time rinsing, first time soaks, second time rinsing, second time is soaked, third time rinses and obtains Fructus Lycopersici esculenti stock breeding seed after drying, wherein, first time rinsing, second time rinsing and third time rinsing all adopt clear water to rinse, the time of second time rinsing and the time of third time rinsing are 15 minutes to 20 minutes, the soak that first time immersion adopts is mass percent is the trisodium phosphate aqueous solution of 10% to 12%, the soak that second time immersion adopts is mass percent is the aqueous hydrochloric acid solution of 1% to 1.5%.
3. the Seed Collecting Methods of Fructus Lycopersici esculenti stock breeding seed according to claim 2, it is characterised in that the time that first time soaks is 20 minutes to 23 minutes;Or/and, the time that second time is soaked is 30 minutes to 32 minutes.
4. the Seed Collecting Methods of the Fructus Lycopersici esculenti stock breeding seed according to claim 1 or 2 or 3, it is characterised in that the tamato fruit of plantation in Fructus Lycopersici esculenti seed farm of gathering, removes the disease pest fruit in Fructus Lycopersici esculenti seed farm, fallen fruits, decayed fruit and hybrid strain.
5. the Seed Collecting Methods of the Fructus Lycopersici esculenti stock breeding seed according to claim 1 or 2 or 3, it is characterized in that in dry run, by seed dehydration 3 minutes to 5 minutes in dewaterer after rinsing and immersion, then, it is placed on yarn bed to spread out seed, and by seed dry in the sun under the sun, after dry in the sun is dry, obtain Fructus Lycopersici esculenti stock breeding seed.
6. the Seed Collecting Methods of Fructus Lycopersici esculenti stock breeding seed according to claim 4, it is characterized in that in dry run, by seed dehydration 3 minutes to 5 minutes in dewaterer after rinsing and immersion, then, it is placed on yarn bed to spread out seed, and by seed dry in the sun under the sun, after dry in the sun is dry, obtain Fructus Lycopersici esculenti stock breeding seed.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107306537A (en) * | 2017-07-27 | 2017-11-03 | 河北省农林科学院经济作物研究所 | A kind of tomato takes the method planted |
CN108811583A (en) * | 2018-06-01 | 2018-11-16 | 山东寿光蔬菜种业集团有限公司 | Fruits and vegetables seed extractor and extraction and the method for handling seed |
CN114402739A (en) * | 2022-02-12 | 2022-04-29 | 江苏省中国科学院植物研究所 | Method for quickly obtaining large amount of high-purity blueberry seeds |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001067102A2 (en) * | 2000-03-06 | 2001-09-13 | The Regents Of The University Of California | Detection of chitin-containing contaminants such as microorganisms |
CN1600075A (en) * | 2003-09-27 | 2005-03-30 | 东北农业大学 | Method for cultivating disease resistance tomato breeds of standing against store and transportation and long shelf life |
CN101069484A (en) * | 2007-06-15 | 2007-11-14 | 东北农业大学 | Method for culturing and preserving tomato male-sterile line |
CN103098589A (en) * | 2013-03-06 | 2013-05-15 | 赵萍 | Method for carrying out fermentation treatment on tomato seeds by complex enzyme |
-
2016
- 2016-02-16 CN CN201610087009.XA patent/CN105706566A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001067102A2 (en) * | 2000-03-06 | 2001-09-13 | The Regents Of The University Of California | Detection of chitin-containing contaminants such as microorganisms |
CN1600075A (en) * | 2003-09-27 | 2005-03-30 | 东北农业大学 | Method for cultivating disease resistance tomato breeds of standing against store and transportation and long shelf life |
CN101069484A (en) * | 2007-06-15 | 2007-11-14 | 东北农业大学 | Method for culturing and preserving tomato male-sterile line |
CN103098589A (en) * | 2013-03-06 | 2013-05-15 | 赵萍 | Method for carrying out fermentation treatment on tomato seeds by complex enzyme |
Non-Patent Citations (2)
Title |
---|
刘光武 等: "番茄采种技术的研究", 《中国蔬菜》 * |
胡雪竹: "番茄种子发芽特性的研究", 《现代农业科技》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107306537A (en) * | 2017-07-27 | 2017-11-03 | 河北省农林科学院经济作物研究所 | A kind of tomato takes the method planted |
CN108811583A (en) * | 2018-06-01 | 2018-11-16 | 山东寿光蔬菜种业集团有限公司 | Fruits and vegetables seed extractor and extraction and the method for handling seed |
CN114402739A (en) * | 2022-02-12 | 2022-04-29 | 江苏省中国科学院植物研究所 | Method for quickly obtaining large amount of high-purity blueberry seeds |
CN114402739B (en) * | 2022-02-12 | 2023-01-03 | 江苏省中国科学院植物研究所 | Method for quickly obtaining large amount of high-purity blueberry seeds |
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