CN105700124B - A kind of microscope illumination system based on LED array - Google Patents
A kind of microscope illumination system based on LED array Download PDFInfo
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- CN105700124B CN105700124B CN201610228592.1A CN201610228592A CN105700124B CN 105700124 B CN105700124 B CN 105700124B CN 201610228592 A CN201610228592 A CN 201610228592A CN 105700124 B CN105700124 B CN 105700124B
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
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- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
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- Microscoopes, Condenser (AREA)
Abstract
The invention belongs to microscope technology field,More particularly to a kind of microscope illumination system based on LED array,Including microscope frame,Fixing device,Attachment means and electric apparatus control apparatus,Microscope frame is provided with eyepiece,Converter,Object lens,Objective table group,Contrast draw back plate group,Lighting device,Focus adjusting mechanism and Jing Bi groups,Lighting device includes aplanatic lens,LED array lamp group,Pcb board and aperture diaphragm group,Lighting device is fixed by fixing device turns into an entirety,The focal length of aplanatic lens is 8~30mm,LED array lamp group pattern is on pcb board,And it is arranged on conjugate planes position of the object lens entrance pupil after aplanatic lens,The LED angle of divergence is 15 °~60 ° in LED array lamp group,LED quantity >=15,Lighting device is connected on microscope frame by attachment means,Lighting device is connected by attachment means with electric apparatus control apparatus.The purpose of the present invention is：Solve the problems, such as that existing microscope illuminator fault of construction brings illumination unevenness etc. many.
Description
Technical field
The invention belongs to microscope technology field, and in particular to a kind of microscope illumination system based on LED array.
Background technology
Microscope optical system includes illuminator and imaging system two parts, and illuminator is critically important one of microscope
Part, the lighting system of illuminator have very important influence on imaging system, more usually can be influenceed with it
Microscopical performance.But illuminator is also that a minimum part is understood in microscope, under many circumstances, a design essence
Good microscope not necessarily obtains good image quality,《Photography Through the Microscope》
John Delly think in one book, there is provided the photomicrograph to academic discussion, exhibition and technical press has 80%~
The correction of 90% light path is incorrect, and the illuminating position of its sample is very poor, and this is one present in microexamination and photomicrography
Extremely serious common fault, it is that the non-adjustment of illuminator is good or do not design mostly the main reason for this problem occur, makes mark
Originally can not obtain caused by sufficiently illuminating.
On the whole, the optimal illumination condition of microscope specimen should be become clear, free from glare, uniformly, colour temperature meet use
It is required that；Illumination zone, illumination aperture are adapted with microscopy imaging system.It is existing in order to obtain best illuminating effect
Some microscope illumination generally use critical illumination or cola illuminations.
Critical illumination is that the optical principle proposed according to Abbe is invented, and light source is imaged in sample by it by collector lens
In plane, the illumination to sample is realized, but in this means of illumination, the uniformity of light source is a highly important condition,
The tengsten lamp of reality, the imperfect problem of Halogen lamp LED generally existing uniformity, from 19th century to 20th century, critical illumination is micro-
Quite varied application has been obtained in mirror, but the problems such as not uniform enough is illuminated due to existing, at present now, critical illumination generally only should
Used in not needing in microscope shoot or more inexpensive microscope.
Cola illumination is into the aperture light of collector lens by light collecting lens, field lens lens by the light source picture of an amplification
At column, by condenser produce an angle it is very big can produce the parallel light cone of sample optimum resolution, and pass through aperture light
Column controls the angle of parallel light cone, that is, the numerical aperture of illuminator, to control unnecessary veiling glare and dazzle, this
Sample, because light source is imaged at the aperture diaphragm of system, even if light source sends is uneven light using cola illumination, regarding
It also will not be uneven to be distributed in.But cola illumination due to optical lens it is a lot, light path is very long, cause it is complicated,
Adjustment to optics brings very big difficulty, and the correctness of optics will directly affect effective sample illumination,
Incorrect correction will severely impact the quality of microscope imaging, make its produce spot, vignette, color spot, contrast it is bad and its
The defects of it is of all kinds.So in order to ensure that illuminator is adapted to the various object lens of imaging system, it need to generally increase illumination
The axial regulating mechanism and radiai adjustment mechanism of system, at the same time, due to microscope in order to ensure be changed without condenser
In the case of when can meet high low power objective simultaneously can optimal use, it can be seen from the principle of conspicuous invariant is drawn, existing product
Illuminator is difficult to meet the requirement of imaging system, therefore, cola illuminator is not only complicated, is needed toward contact or increases hair
Glass uses a variety of collector lenses or abandons illumination zone or the demand of illumination aperture.
Meanwhile the light source such as existing microscopic system generally use tengsten lamp, fluorescent lamp, Halogen lamp LED, LED, tengsten lamp,
Commonly there is the problems such as short life, heating is big, light inner feelings is fast in fluorescent lamp, Halogen lamp LED, LED is due to small volume, power consumption is low, uses
Long lifespan, high brightness, it is low in calories, sturdy and durable the advantages that it is now more and more common be applied to illumination, but there is spectrum in LED
The problems such as continuity is inadequate, colour rendering index is inadequate.
In addition, during using micro- sem observation sample, due to the difference of sample nature, only there is certain list
The microscope of one lighting system, when observing sample of different nature, be extremely difficult to preferable illuminating effect and into
As effect.The mode that general microscope can all provide a variety of different illuminations selects for user, such as in addition to the transillumination of routine,
Annular oblique illumination also required for dark-ground illumination, phase contrast observation etc., but when switching lighting system, step is comparatively laborious, such as
When switching to transmission dark field, except switching object lens, the details in a play not acted out on stage, but told through dialogues draw back plate according to corresponding to the selection of the parameter of object lens is also needed, is easily gone out
It is wrong.
In Publication No. CN104459969A patent, describe a kind of biomicroscope based on engineering diffuser and shine
Bright system, it includes：LED lamp, convergent lens, engineering diffuser, objective table, LED provide high
The illuminating bundle of intensity, convergent lens are received and assemble illuminating bundle, and engineering diffuser is controlled to illuminating bundle, and control is shone
The spatial distribution and intensity profile of Mingguang City's beam so that the illuminance of illuminating bundle equably project placed on objective table it is to be checked
The biological sample of survey.In Publication No. CN102789048A patent, describe one kind and be used for microscopical cold light source lighting
System, it includes：Housing, LED, aspherical condenser, barrel assembly etc., the inside of housing are provided with LED illuminating sources, aspheric
The light that face condenser aggregation LED is sent, barrel assembly are used to fix other parts.In Publication No. CN2769911Y patent
In, a kind of lighting device of the biomicroscope using light emitting diode is described, it consolidates in the following of objective table and with handel
Surely light source loading platform is connected with, LED socket is fixedly connected with the light source loading platform, LED socket activity
Connecting luminous diode.Said lighting system all exist can not meet simultaneously microscope height observe when numerical aperture, shine
The requirement of bright scope, there is also certain deficiency on maintaining uniform illumination, meanwhile, the lighting system of said lighting system is single, only
With light field function, the observation requirement of various samples can not be met.
The content of the invention
The purpose of the present invention is：A kind of microscope illumination system based on LED array is aimed to provide, it is existing aobvious to solve
Micro mirror illuminator structure imperfect tape, which comes illumination unevenness, numerical aperture and illumination zone, can not meet requirement, lighting system list
First, the problem of function is single etc. many.
To realize above-mentioned technical purpose, the technical solution adopted by the present invention is as follows：A kind of microscope based on LED array shines
Bright system, including microscope frame, described microscope frame are provided with eyepiece, converter, object lens, objective table group, contrast and pushed away
Arm-tie group, lighting device, focus adjusting mechanism and Jing Bi groups, in addition to fixing device, attachment means and electric apparatus control apparatus, it is described
Lighting device includes aplanatic lens, LED array lamp group, pcb board and aperture diaphragm group, and described lighting device is filled by fixed
Putting fixation turns into an entirety, and the focal length of the aplanatic lens is 8~30mm, and described LED array lamp group pattern is in pcb board
Go up and be arranged on conjugate planes position of the object lens entrance pupil after aplanatic lens, the LED angle of divergence in the LED array lamp group
For 15 °~60 °, LED quantity >=15, lighting device is connected on microscope frame by described attachment means, described
Lighting device is connected by attachment means with electric apparatus control apparatus, and described electric apparatus control apparatus includes drive circuit, microcontroller
Device and electric switch.
Using the invention of above-mentioned technical proposal, it is possible to achieve improve cola to sample a wide range of, large aperture, high brightness
Illumination, reaches the Uniform Illumination effect to sample, user without center adjustment, need not be adjusted axially with regard to best illumination can be reached
Effect.
Further, the LED in described LED array lamp group be white light LEDs, monochromatic LED, it is multi-colored led in any one.
Such design, lighting system and vdiverse in function can meet the needs of different.
Further, the focal length of the aplanatic lens is 15mm；In the LED array lamp group LED angle of divergence be 25 °,
LED quantity is 37.
Such design, numerical aperture and illumination zone are preferable, and imaging effect is preferable.
Further, it is described multi-colored led for white light LEDs and amber LED permutation and combination.
Such design, colour temperature is soft, higher to the resolution ratio of sample details.
Further, it is described multi-colored led for white light LEDs and blue-green LED permutation and combination.
Such design, illumination spectrum continuity are preferable, it is possible to achieve to the more preferable color rendition effect of sample.
Further, described aplanatic lens is Fresnel Lenses, and described lighting device also includes protective glass, Fei Nie
Seat, collector lens base, lower cover and spacer ring under your the lens seat of honour, collector lens, described fixing device include crossed countersunk head
Screw, positioning screw, compression spring, interior hexagonal holding screw, cross small pan head screw and steel ball, described protective glass and phenanthrene
Nie Er lens are fixed in the Fresnel Lenses seat of honour, and described aperture diaphragm group is by cross small pan head screw lock in Fresnel
Under the lens seat of honour and Fresnel Lenses among seat, described compression spring and steel ball are located under Fresnel Lenses in seat, and are passed through
Interior hexagonal holding screw locking, described LED array lamp are mounted in Fresnel Lenses base, and described lower cover and spacer ring lead to
Crossed countersunk head screw lock is crossed, described lower cover offers the power supply string holes being connected with electric apparatus control apparatus.
Such design, when switching different object lens, it is not necessary to which axial direction, radiai adjustment, which can easily just meet to use, wants
Ask, reach uniform illuminating effect, meanwhile, adjustment aperture light bar group makes using different marks can change illumination aperture
Originally, during different object lens, optimal contrast is attained by, the technical requirements to user of service is greatly reduced, makes use more square
Just, quality is more stable, and simple in construction, easy to assembly, and independent annex can be integrally formed, can substantially reduce product manufacturing into
This.
Further, described aplanatic lens is non-spherical lens.
Such design, manufacturing cost is relatively low, is convenient for people to use.
Further, described aplanatic lens is multi-disc lens combination, including upper concentration piece, middle concentration piece and lower optically focused
Piece, described lighting device also include seat, optically focused under No.1 spacer ring, the multi-disc lens seat of honour, No. two spacer rings, trim ring, collector lenses
Lens mount, lower cover and spacer ring, described fixing device include crossed countersunk head screw, positioning screw, compression spring, interior hexagonal
Holding screw, cross small pan head screw and steel ball, described upper concentration piece, middle concentration piece and lower concentration piece by No. two spacer rings,
No.1 spacer ring is separated in the multi-disc lens seat of honour, and is locked by trim ring, and described aperture diaphragm group passes through small by cross
Pan head screw is locked under the multi-disc lens seat of honour and collector lens among seat, and described compression spring and steel ball are located at collector lens
In lower seat, and locked by interior hexagonal holding screw, described LED array lamp is mounted in collector lens base, under described
Cover plate and spacer ring offer the power line being connected with electric apparatus control apparatus by crossed countersunk head screw lock, described lower cover
Hole.
Such design, when switching different object lens, it is not necessary to which axial direction, radiai adjustment, which can easily just meet to use, wants
Ask, reach uniform illuminating effect, meanwhile, adjustment aperture light bar group makes using different marks can change illumination aperture
Originally, during different object lens, optimal contrast is attained by, the technical requirements to user of service is greatly reduced, makes use more square
Just, quality is more stable, and simple in construction, easy to assembly, and independent annex can be integrally formed, can substantially reduce product manufacturing into
This.
Further, described electric apparatus control apparatus is connected with object lens.
Such design, can be according to the situation automatic regulating lightness of the object lens of actual use and aperture.
Further, described electric apparatus control apparatus is connected with computer.
Such design, it is possible to achieve illumination condition is controlled by computer.
Brief description of the drawings
The present invention can be further illustrated by the nonlimiting examples that accompanying drawing provides；
Fig. 1 is a kind of structural representation of the embodiment of the microscope illumination system based on LED array of the present invention；
When Fig. 2 is that aplanatic lens is Fresnel Lenses in a kind of microscope illumination system based on LED array of the present invention
The structural representation of lighting device；
Fig. 3 is that aplanatic lens is multi-disc lens combination in a kind of microscope illumination system based on LED array of the present invention
When lighting device structural representation；
Fig. 4 is that the LED in LED array lamp group is white light in a kind of microscope illumination system based on LED array of the present invention
LED or structural representation during monochromatic LED；
Fig. 5 is that the LED in LED array lamp group is polychrome in a kind of microscope illumination system based on LED array of the present invention
Structural representation during LED；
Fig. 6 is the structural representation of Fresnel Lenses in Fig. 2.
Main element symbol description is as follows：
11. eyepiece, 12. converters, 13. object lens, 14. samples, 15. objective table groups, 16. contrast draw back plate groups, 17. illuminations
Device, 18. focus adjusting mechanisms, 19. Jing Bi groups, 20. protective glass, 21. Fresnel Lenses, the 22. Fresnel Lenses seats of honour, 23. holes
Footpath light bar group, seat under 24. collector lenses, 25.LED array lamp groups, 26. collector lens bases, 27. lower covers, 28. spacer rings, 29.
Crossed countersunk head screw, 30. positioning screws, 31. compression springs, hexagonal holding screw in 32., 33. cross small pan head screws, 34.
Steel ball, concentration piece on 35., 36. No.1 spacer rings, concentration piece in 37., the 38. multi-disc lens seats of honour, 39. No. two spacer rings, 40. times poly-
Mating plate, 41. trim rings, 42.PCB plates, 43. same sex LED light sources, 44. first LED light sources, 45. second LED light sources
Embodiment
In order that the present invention may be better understood in those skilled in the art, with reference to the accompanying drawings and examples to this hair
Bright technical scheme further illustrates.
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4, Fig. 5 and Fig. 6, a kind of microscope illumination system based on LED array of the invention
System, including microscope frame, fixing device, attachment means and electric apparatus control apparatus, microscope frame are provided with eyepiece 11, conversion
Device 12, object lens 13, objective table group 15, contrast draw back plate group 16, lighting device 17, focus adjusting mechanism 18 and Jing Bi groups 19, illumination dress
Put 17 and pass through fixing device including aplanatic lens, LED array lamp group 25, pcb board 42 and aperture diaphragm group 23, lighting device 17
Fixation turns into an entirety, and the focal length of aplanatic lens is 8~30mm, preferably 15mm, and the array of LED array lamp group 25 is in pcb board
On 42 and it is arranged on conjugate planes position of the entrance pupil of object lens 13 after aplanatic lens, LED diverging in LED array lamp group 25
Angle is 15 °~60 °, and preferably 25 °, preferably LED quantity >=15,37, now, numerical aperture and illumination zone are preferable, into
Picture effect is preferable, and the LED in LED array lamp group 25 can be white light LEDs or monochromatic LED or multi-colored led, lighting system and function
It is various, can meet the needs of different.When LED is white light LEDs or monochromatic LED, using same sex LED light source 43, generally, polychrome
When LED is white light LEDs and amber LED permutation and combination, colour temperature is soft, and multi-colored led is white light LEDs and blue-green LED arrangement groups
During conjunction, spectrum continuity is preferable, and the first LED light source 44, the second LED light source 45 is respectively adopted.Attachment means are by lighting device 17
It is connected on microscope frame, lighting device 17 is connected by attachment means with electric apparatus control apparatus, and electric apparatus control apparatus includes
Drive circuit, microcontroller and electric switch.
When aplanatic lens uses Fresnel Lenses 21, lighting device 17 also includes protective glass 20, Fresnel Lenses
Seat 24, collector lens base 26, lower cover 27 and spacer ring 28 under the seat of honour 22, collector lens, fixing device include crossed countersunk head spiral shell
Nail 29, positioning screw 30, compression spring 31, interior hexagonal holding screw 32, cross small pan head screw 33 and steel ball 34, protective glass
20 and Fresnel Lenses 21 be fixed in the Fresnel Lenses seat of honour 22, aperture diaphragm group 23 is locked by cross small pan head screw 33
Under the Fresnel Lenses seat of honour 22 and Fresnel Lenses among seat 24, compression spring 31 and steel ball 34 are located at seat under Fresnel Lenses
In 24, and locked by interior hexagonal holding screw 32, LED array lamp group 25 is in Fresnel Lenses base 26, lower cover 27
Locked with spacer ring 28 by crossed countersunk head screw 29, lower cover 27 offers the power supply string holes being connected with electric apparatus control apparatus.
When switching different object lens 13, it is not necessary to which axial direction, radiai adjustment can easily just meet requirement, reach uniform
Illuminating effect, meanwhile, adjustment aperture light bar group 23 makes using different sample 14, different can change illumination aperture
Object lens 13 when, be attained by optimal contrast, greatly reduce to the technical requirements of user of service, allow using more convenient, matter
Measure it is more stable and simple in construction, it is easy to assembly, independent annex can be integrally formed, cost of goods manufactured can be substantially reduced.
When aplanatic lens uses non-spherical lens, manufacturing cost can be reduced.
When aplanatic lens uses multi-disc lens combination, multi-disc lens combination includes upper concentration piece 35, middle concentration piece 37
With lower concentration piece 40, lighting device 17 also includes No.1 spacer ring 36, the multi-disc lens seat of honour 38, two spacer ring 39, trim ring 41, optically focused
Seat 24, collector lens base 26, lower cover 27 and spacer ring 28 under lens, fixing device include crossed countersunk head screw 29, positioning spiral shell
Nail 30, compression spring 31, interior hexagonal holding screw 32, cross small pan head screw 33 and steel ball 34, upper concentration piece 35, middle concentration piece
37 and lower concentration piece 40 separated by No. two spacer rings 39, No.1 spacer ring 36 in the multi-disc lens seat of honour 38, and pass through trim ring 41
Locking, aperture diaphragm group 23 by cross small pan head screw 33 by being locked at seat 24 under the multi-disc lens seat of honour 38 and collector lens
Centre, compression spring 31 and steel ball 34 are located under collector lens in seat 24, and are locked by interior hexagonal holding screw 32, LED battle arrays
Row lamp group 25 is in collector lens base 26, and lower cover 27 and spacer ring 28 are locked by crossed countersunk head screw 29, lower cover 27
Offer the power supply string holes being connected with electric apparatus control apparatus.
When switching different object lens 13, it is not necessary to which axial direction, radiai adjustment can easily just meet requirement, reach uniform
Illuminating effect, meanwhile, adjustment aperture light bar group 23 makes using different sample 14, different can change illumination aperture
Object lens 13 when, be attained by optimal contrast, greatly reduce to the technical requirements of user of service, allow using more convenient, matter
Measure it is more stable and simple in construction, it is easy to assembly, independent annex can be integrally formed, cost of goods manufactured can be substantially reduced.
It is pointed out that electric apparatus control apparatus is connected with object lens 13, primary concern is that, can be according to actual use
The situation automatic regulating lightness of object lens 13 and aperture.In fact, can also be depending on actual conditions.
In addition, electric apparatus control apparatus is connected with computer, it is possible to achieve controls illumination condition by computer.In fact,
Can also be depending on actual conditions.
In addition, a diameter of 3mm of LED light source, the power about 0.06W per LEDs, 37 LEDs array of source layout exist
In PCB 42, and it is in four rings, the first ring of the form that six sections of outer shroud, wherein center is made up of 1 LEDs light source,
For the lighting demand for the object lens 13 for realizing low numerical aperture；Second ring is made up of 6 LEDs light sources, and each section includes 1 LEDs
Light source, the lighting demand for the object lens 13 of numerical aperture in realization；3rd ring is made up of 12 LEDs light sources, and each section includes 2
LEDs light source, the lighting demand of the object lens 13 for realizing high-NA；Fourth Ring is made up of 18 LEDs light sources, Mei Yiduan
Comprising 3 LEDs light sources, for realizing the lighting demand of dark field microscope.It can so be realized by electric apparatus control apparatus different
Ring, the switch and luminance state of different sections, realizes the regulation of illumination aperture, can also realize the microscopies such as details in a play not acted out on stage, but told through dialogues.
When the first LED light source 44, the second LED light source 45 simultaneously in the presence of, it is most of to use the first LED light source 44, part
Using the second LED light source 45.
Further, the different annulus of Fresnel Lenses 21 use different radius of curvature, so as to realize the elimination to spherical aberration,
And the minimum of product is realized as far as possible, so as to greatly simplify structure, reduce cost.
The principle and its effect of the merely exemplary explanation present invention of above-described embodiment, not for the limitation present invention.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and scope without prejudice to the present invention to above-described embodiment.Cause
This, all those of ordinary skill in the art without departing from disclosed spirit with being completed under technological thought
All equivalent modifications or change, should by the present invention claim be covered.
Claims (7)
1. a kind of microscope illumination system based on LED array, including microscope frame, described microscope frame is provided with mesh
Mirror (11), converter (12), object lens (13), objective table group (15), contrast draw back plate group (16), lighting device (17), focusing machine
Structure (18) and Jing Bi groups (19), it is characterised in that：Also include fixing device, attachment means and electric apparatus control apparatus, described photograph
Bright device (17) includes aplanatic lens, LED array lamp group (25), pcb board (42) and aperture diaphragm group (23), described illumination
Device (17) is fixed by fixing device turns into an entirety, and the focal length of the aplanatic lens is 8~30mm, described LED
Array lamp group (25) array is on pcb board (42) and is arranged on conjugate planes position of object lens (13) entrance pupil after aplanatic lens
Put, the LED angle of divergence is 15 °~60 °, LED quantity >=15 in the LED array lamp group (25), described attachment means
Lighting device (17) is connected on microscope frame, described lighting device (17) passes through attachment means and electric apparatus control apparatus
Connection, described electric apparatus control apparatus include drive circuit, microcontroller and electric switch；
Described aplanatic lens is Fresnel Lenses (21), and described lighting device (17) also includes protective glass (20), phenanthrene
Seat (24), collector lens base (26), lower cover (27) and spacer ring (28), described under the Nie Er lens seat of honour (22), collector lens
Fixing device include crossed countersunk head screw (29), positioning screw (30), compression spring (31), interior hexagonal holding screw (32),
It is saturating that cross small pan head screw (33) and steel ball (34), described protective glass (20) and Fresnel Lenses (21) are fixed on Fresnel
In the mirror seat of honour (22), described aperture diaphragm group (23) is locked at the Fresnel Lenses seat of honour by cross small pan head screw (33)
(22) and under Fresnel Lenses seat (24) is middle, and described compression spring (31) and steel ball (34) are located at seat under Fresnel Lenses
(24) in, and locked by interior hexagonal holding screw (32), described LED array lamp group (25) is located at Fresnel Lenses base
(26) in, described lower cover (27) and spacer ring (28) are locked by crossed countersunk head screw (29), and described lower cover (27) is opened
Provided with the power supply string holes being connected with electric apparatus control apparatus.
A kind of 2. microscope illumination system based on LED array according to claim 1, it is characterised in that：Described LED
LED in array lamp group (25) be white light LEDs, monochromatic LED, it is multi-colored led in any one.
A kind of 3. microscope illumination system based on LED array according to claim 1 or 2, it is characterised in that：It is described to disappear
The focal length of Lens with Spherical Aberration is 15mm；In the LED array lamp group (25) the LED angle of divergence be 25 °, LED quantity be 37.
A kind of 4. microscope illumination system based on LED array according to claim 2, it is characterised in that：Described is more
Color LED is white light LEDs and amber LED permutation and combination.
A kind of 5. microscope illumination system based on LED array according to claim 2, it is characterised in that：Described is more
Color LED is white light LEDs and blue-green LED permutation and combination.
A kind of 6. microscope illumination system based on LED array according to claim 1, it is characterised in that：Described electricity
Device control device is connected with object lens (13).
A kind of 7. microscope illumination system based on LED array according to claim 1 or 6, it is characterised in that：Described
Electric apparatus control apparatus is connected with computer.
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| DE102016124612A1 (en) * | 2016-12-16 | 2018-06-21 | Carl Zeiss Microscopy Gmbh | Segmented optics for a lighting module for angle-selective lighting |
| CN108795762A (en) * | 2018-07-25 | 2018-11-13 | 太平洋康泰科学仪器（济南）有限公司 | A kind of time difference imaging culture systems and its method |
| CN110927949A (en) * | 2019-12-12 | 2020-03-27 | 新凤鸣集团湖州中石科技有限公司 | Processing method and processing system of polyester filament fiber section analyzer |
| CN111812833A (en) * | 2020-08-04 | 2020-10-23 | 东南大学江北新区创新研究院 | Low-disturbance microscope for organ chip imaging and imaging method thereof |
| CN117147551B (en) * | 2023-10-30 | 2024-02-20 | 中国科学院苏州生物医学工程技术研究所 | Chromosome scanning imaging method and device |
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