CN105696195A - Preparation method of carnosol and chitosan composite nanometer fiber mat - Google Patents
Preparation method of carnosol and chitosan composite nanometer fiber mat Download PDFInfo
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- CN105696195A CN105696195A CN201610208095.5A CN201610208095A CN105696195A CN 105696195 A CN105696195 A CN 105696195A CN 201610208095 A CN201610208095 A CN 201610208095A CN 105696195 A CN105696195 A CN 105696195A
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4382—Stretched reticular film fibres; Composite fibres; Mixed fibres; Ultrafine fibres; Fibres for artificial leather
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
- C07D493/08—Bridged systems
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D1/00—Treatment of filament-forming or like material
- D01D1/02—Preparation of spinning solutions
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D1/00—Treatment of filament-forming or like material
- D01D1/10—Filtering or de-aerating the spinning solution or melt
- D01D1/103—De-aerating
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0069—Electro-spinning characterised by the electro-spinning apparatus characterised by the spinning section, e.g. capillary tube, protrusion or pin
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0092—Electro-spinning characterised by the electro-spinning apparatus characterised by the electrical field, e.g. combined with a magnetic fields, using biased or alternating fields
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F6/00—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof
- D01F6/96—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof from other synthetic polymers
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Mechanical Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Manufacturing & Machinery (AREA)
- Artificial Filaments (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a preparation method of a carnosol and chitosan composite nanometer fiber mat. The method comprises the steps that chitosan is dissolved in a formic acid solution, stirring is conducted, and a chitosan solution is obtained; carnosol is mixed with the chitosan solution, stirring and ultrasonic degassing are conducted, a carnosol and chitosan solution is obtained, electrostatic spinning is conducted, and the carnosol and chitosan composite nanometer fiber mat is obtained. The method is simple, easy to carry out and easy to industrialize, and the obtained carnosol and chitosan composite nanometer fiber mat can be used for the fields of wound dressings and the like and is excellent in antibacterial effect and wide in application prospect.
Description
Technical field
The invention belongs to anti-bacterial high-molecule nano-fiber material preparation field, particularly to the preparation method of a kind of carnosol and chitosan composite nano fibre felt。
Background technology
Nanofiber definition have narrow sense, broad sense point。The nanofiber of narrow sense refers to that diameter is nanoscale scope, is namely defined as the fiber that diameter is 1-100nm。As long as the nanofiber of broad sense refers to includes nanostructured in fiber, and impart again new physical property, then can put the category of nanofiber under。Electrostatic spinning is to prepare continuous nano-fibre now, and is expected to the important method of industrialized production。Electrostatic spinning is by several thousand to several ten thousand volt high-pressure electrostatics on polymer solution or melt band, and charged polymer liquid drops in and is accelerated at the Taylor conical point of capillary tube under the active force of electric field。When electric field force is sufficiently large, polymer drop can overcome surface tension to form injection thread。Thread is solvent evaporation or solidification in course of injection, finally falls on the reception device, forms the fiber felt of similar non-woven cloth-like。The fiber prepared with method of electrostatic spinning is much thinner than traditional method, and its diameter is typically between tens nanometer to hundreds of nanometers。
Utilize the fiber felt that nanofiber is made to have significantly high porosity, therefore there is the feature of highly-breathable, add nanofiber and there is big surface area, be commonly used to make medical dressing to promote that wound restores。The microscopic dimensions solving existing medical dressing is general relatively big, when contacting with wound in hemostasis, is unfavorable for the problem absorbing rapidly and suppressing to infect of transudate。Nanofiber mats make medical dressing for treatment of wounds time be conducive to the evaporation of wound fluid, avoid wound surface hydrops, be conducive to oxygen to pass through simultaneously, it is also beneficial to wound tissue Cellular respiration, the growth of epithelial tissue and regeneration, the healing of wound can be dramatically speeded up, by adding antimicrobial component, effectively suppress wound infection further。
Antibacterial used in anti-biotic material can be divided into inorganic antiseptic, organic antibacterial agent, natural antibacterial agent etc.。Wherein with silver ion be representative the chemical property of inorganic antiseptic material own active, be easily reduced into the elemental silver of black when light, heat and ultraviolet, limit its range of application。The use of organic synthesis antibacterial makes vegeto-animal pathogen develop immunity to drugs, and affects health。With the antibacterial being effective ingredient from the plant extract such as Herba Taraxaci, Salvia japonica Thunb., have that source is wide, toxicity is low, not easily cause Drug resistance, nature is prone to degraded, to advantages such as environmental effect are little。Therefore, vegetable active antimicrobial component is increasingly becoming the important channel developing safe efficient antibacterial agent。Wherein phenolic antiseptic can pass through to destroy bacterial cell membrane, makes Cytoplasmic inclusions ooze out, it is suppressed that bacterial dehydrogenase and oxidasic effect, affects the metabolism of antibacterial, thus playing the effect of sterilization。
For these reasons, preparing novel carnosol composite nanometer fiber felt significant, current Research Literature and patent related content are few。Modern pharmacological research shows, staphylococcus aureus, escherichia coli, bacillus subtilis and Hansenula anomala bacterium are all had inhibitory action in various degree by carnosol, have broad-spectrum antimicrobial effect。
Summary of the invention
The technical problem to be solved is to provide a kind of carnosol and the preparation method of chitosan composite nano fibre felt, the method is simple, being prone to industrialization, gained carnosol composite nanometer fiber felt can be used for the fields such as wound dressing, and antibacterial effect is outstanding。
A kind of carnosol of the present invention and the preparation method of chitosan composite nano fibre felt, including:
(1) chitosan is dissolved in formic acid, stirring, obtain the chitosan solution that concentration is 0.03～0.05g/ml；
(2) extract carnosol from the raw material containing carnosol, then mix with the chitosan solution in step (1), stirring, ultrasonic degassed, obtain carnosol/chitosan solution；Wherein, carnosol is 1: 99～1: 95 with chitosan mass ratio；
(3) carnosol/chitosan solution in step (2) is carried out electrostatic spinning, obtain carnosol and chitosan composite nano fibre felt；Wherein, the average diameter of nanofiber is 100-700nm。
In described step (1), the mass body volume concentrations of formic acid is 88%。
The extracting method of carnosol in described step (2): extracted 2-5 time by the raw material lipotropy organic solvent containing carnosol, united extraction liquid, concentration obtains the extractum containing carnosol or solid；Dissolving with the hydrophilic organic solvent of 10-90%, filter precipitation, filtrate carries out Macroporous Adsorption Resin process, obtains precipitate containing carnosol；Precipitate dissolves with the lipotropy organic solvent of mixing again, places, and precipitates out precipitation；Filtration precipitates to obtain carnosol, i.e. carnosol。
The described raw material containing carnosol, refer to Herba Rosmarini Officinalis, Salvia japonica Thunb., Radix Salviae Miltiorrhizae etc. be containing the plant of carnosol。
Described lipotropy organic solvent, refers to that the organic solvent of layering can occur after mixing with water lower than 100 degrees Celsius boiling point, such as petroleum ether, benzene, ether, ethanol, dichloromethane, acetone, chloroform, ethyl acetate, normal hexane etc.。
Described hydrophilic organic solvent, refer to boiling point lower than 100 degrees Celsius, water-miscible organic solvent, such as acetone, ethanol, methanol, isopropanol etc.。Described 10-90% is percent by volume aqueous solution；More superior is 40-70% percent by volume aqueous solution。
The lipotropy organic solvent of described mixing is at least two in petroleum ether, benzene, ether, ethanol, dichloromethane, acetone, chloroform, ethyl acetate and normal hexane。
Described macroporous adsorbent resin, refer to D101, AB-8, HZ818 therein any one。
In described step (2), the time of stirring is 1～2h；The ultrasonic degassed time is 2～10min。
In described step (3), the condition of electrostatic spinning is: voltage is 18～35kV, and receiving range is 8～22cm, and spinning speed is 0.4ml/h, and spinneret orifice internal diameter 0.7mm, spinning at room temperature carries out。
In described step (3), the antibiotic rate of escherichia coli and staphylococcus aureus is reached more than 95% by carnosol and chitosan composite nano fibre felt。
The present invention utilizes continuous print, relatively easy electrostatic spinning technique, obtains carnosol and chitosan composite nano fibre felt。
The method of the present invention is simple, it is easy to industrialization, and gained carnosol composite nanometer fiber felt can be used for the fields such as wound dressing, and antibacterial effect is outstanding, has a extensive future。
Beneficial effect
(1) chitosan high polymer used by the present invention, is natural polymer, and the stock number in nature is only second to cellulose, and affinity is good, cheap, less costly；
(2) in the present invention, carnosol institute expense is few, and preparation is simple, fast；
(3) the nanofiber mats fibre diameter of gained of the present invention and hole are little, and specific surface area is big, and anti-virus and antibiotic property are strong；
(4) the inventive method manufacturing process is simple, it is easy to large-scale production, has good application and commercial promise。
Accompanying drawing explanation
Fig. 1 is carnosol and chitosan composite nano fibre felt scanning electron microscope (SEM) photograph in embodiment 3。
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further。Should be understood that these embodiments are merely to illustrate the present invention rather than restriction the scope of the present invention。In addition, it is to be understood that after having read the content that the present invention lectures, the present invention can be made various changes or modifications by those skilled in the art, and these equivalent form of values fall within the application appended claims limited range equally。
Embodiment 1
Take dry Herba Rosmarini Officinalis branch and leaf 2000g, add dichloromethane 10L, reflux, extract, 3 times, each 3 hours, merge 3 extracting solution, concentration and recovery dichloromethane, obtain extractum；Extractum is dissolved with the ethanol of 60%, is filtered to remove insoluble matter, obtains 1000ml mother solution as adsorption liquid, adsorb with 500mlAB-8 macroporous adsorbent resin, take off by 1000ml purified water through wash after having adsorbed, then with 2000ml80% alcoholic solution eluting, eluent is divided into 6 sections, 4 parts in the middle of merging；The eluent merged is evaporated to 150ml, and add water 200ml, and stirring precipitates out precipitation, places 5 hours；The precipitation being centrifuged containing carnosol；Precipitation 150ml dichloromethane dissolves, and is added thereto to 200ml petroleum ether, and stirring precipitates out precipitation；Filter to obtain carnosol。
Being dissolved in by 0.6 gram of chitosan in 15ml formic acid (mass body volume concentrations 88%), magnetic agitation, to being completely dissolved, obtains the chitosan solution that concentration is 0.04g/ml。
Taking 0.006 gram of carnosol join in prepared chitosan solution and mix, magnetic agitation 1 hour is until obtaining homogeneous solution, and ultrasonic degassing time is 5min。
Electrostatic spinning process parameter is: orifice diameter is 0.7mm, voltage is 26kV, and syringe pump fltting speed is 0.4ml/h, adopts aluminium foil to receive, receiving range is 15cm, and under room temperature, spinning obtains carnosol and the chitosan composite nano fibre felt that nanofiber average diameter is 217nm；
Anti-microbial property is evaluated: according to GB/T20944.3-2008, adopts succusion detection by quantitative, and with control sample, carnosol and chitosan composite nano fibre felt sample are respectively charged into finite concentration, and (viable bacteria number is 3x105In the conical flask of experiment bacterium solution CFU/mL), vibrating 18 hours at 24 DEG C ± 1 DEG C, measure bacterium solution viable bacteria concentration before vibration and after vibration in conical flask, calculate antibiotic rate, evaluate the antibacterial effect of sample with this, evaluation result is in Table 1。
Embodiment 2
Weigh Radix Salviae Miltiorrhizae extract 500g, dissolve with the ethanol of 3000ml50%, be filtered to remove insoluble matter, mother solution adsorbs with 1000mlD101 macroporous adsorbent resin, takes off by 2000ml purified water through wash after having adsorbed, then with 2000ml90% alcoholic solution eluting, eluent is divided into 6 sections, 4 parts in the middle of merging；The eluent merged is evaporated to 500ml, and add water 500ml, and stirring precipitates out precipitation, places 3 hours；Centrifugal, obtain precipitation containing carnosol；Precipitation uses 200ml acetic acid ethyl dissolution, is added thereto to 150ml petroleum ether, stirring, precipitates out precipitation；Filter to obtain active antibacterial ingredient-carnosol。
Being dissolved in by 0.75 gram of chitosan in 15ml formic acid (mass body volume concentrations 88%), magnetic agitation, to being completely dissolved, obtains the chitosan solution that concentration is 0.05g/ml。
Taking 0.008 gram of carnosol join in prepared chitosan solution and mix, magnetic agitation 2 hours is until obtaining homogeneous solution, and ultrasonic degassing time is 10min。
Nano wire is received with aluminium foil or cloth, electrostatic spinning process parameter is: orifice diameter is 0.7mm, voltage is 26kV, syringe pump fltting speed is 0.4ml/h, employing aluminium foil receives, receiving range is 15cm, and under room temperature, spinning obtains carnosol and the chitosan composite nano fibre felt that nanofiber average diameter is 464nm。
Anti-microbial property is evaluated: according to GB/T20944.3-2008, adopts succusion detection by quantitative, and with control sample, carnosol and chitosan composite nano fibre felt sample are respectively charged into finite concentration, and (viable bacteria number is 3x105In the conical flask of experiment bacterium solution CFU/ml), vibrating 18 hours at 24 DEG C ± 1 DEG C, measure bacterium solution viable bacteria concentration before vibration and after vibration in conical flask, calculate antibiotic rate, evaluate the antibacterial effect of sample with this, evaluation result is in Table 1。
Embodiment 3
Take the Salvia japonica Thunb. 2000g of clean dry, add dichloromethane 10L, reflux, extract, 3 times, each 3 hours, merge 3 extracting solution, concentration and recovery dichloromethane, obtain extractum；Extractum is dissolved with the ethanol of 60%, is filtered to remove insoluble matter, obtains 1000ml mother solution as adsorption liquid, adsorb with 500mlAB-8 macroporous adsorbent resin, take off by 1000ml purified water through wash after having adsorbed, then with 2000ml80% alcoholic solution eluting, eluent is divided into 6 sections, 4 parts in the middle of merging；The eluent merged is evaporated to 150ml, and add water 200ml, and stirring precipitates out precipitation, places 5 hours；Centrifugal, obtain precipitation containing carnosol；Precipitation 150ml dichloromethane dissolves, and is added thereto to 200ml petroleum ether, and stirring precipitates out precipitation；Filter to obtain active antibacterial ingredient-carnosol。
Being dissolved in by 0.3 gram of chitosan in 10ml formic acid (mass body volume concentrations 88%), magnetic agitation, to being completely dissolved, obtains the chitosan solution that concentration is 0.05g/ml。
Taking 0.003 gram of carnosol join in prepared chitosan solution and mix, magnetic agitation 2 hours is until obtaining homogeneous solution, and ultrasonic degassing time is 5min。
Nano wire is received with aluminium foil or cloth, electrostatic spinning process parameter is: orifice diameter is 0.7mm, voltage is 26kV, syringe pump fltting speed is 0.4ml/h, employing aluminium foil receives, receiving range is 15cm, and under room temperature, spinning obtains carnosol and the chitosan composite nano fibre felt that nanofiber average diameter is 337nm, and scanning electron microscope (SEM) photograph is as shown in Figure 1。
Anti-microbial property is evaluated: according to GB/T20944.3-2008, adopts succusion detection by quantitative, and with control sample, carnosol and chitosan composite nano fibre felt sample are respectively charged into finite concentration, and (viable bacteria number is 3x105In the conical flask of experiment bacterium solution CFU/ml), vibrating 18 hours at 24 DEG C ± 1 DEG C, measure bacterium solution viable bacteria concentration before vibration and after vibration in conical flask, calculate antibiotic rate, evaluate the antibacterial effect of sample with this, evaluation result is in Table 1。
The anti-microbial property of table 1 carnosol and chitosan composite nano fibre felt
Claims (8)
1. a preparation method for carnosol and chitosan composite nano fibre felt, including:
(1) chitosan is dissolved in formic acid solution, stirring, obtain the chitosan solution that concentration is 0.03～0.05g/ml；
(2) extract carnosol from the raw material containing carnosol, then mix with the chitosan solution in step (1), stirring, ultrasonic degassed, obtain carnosol/chitosan solution；Wherein, carnosol is 1: 99～1: 95 with chitosan mass ratio；
(3) carnosol/chitosan solution in step (2) is carried out electrostatic spinning, obtain carnosol and chitosan composite nano fibre felt。
2. the preparation method of a kind of carnosol according to claim 1 and chitosan composite nano fibre felt, it is characterised in that in described step (1), the mass body volume concentrations of formic acid solution is 88%。
3. the preparation method of a kind of carnosol according to claim 1 and chitosan composite nano fibre felt, it is characterized in that, the extracting method of carnosol in described step (2): the raw material lipotropy organic solvent containing carnosol is extracted 2-5 time, united extraction liquid, concentration obtains the extractum containing carnosol or solid；Dissolving with the hydrophilic organic solvent of 10-90%, filter precipitation, filtrate carries out Macroporous Adsorption Resin process, obtains precipitate containing carnosol；Precipitate dissolves with the lipotropy organic solvent of mixing again, places, and precipitates out precipitation；Filtration precipitates to obtain carnosol。
4. the preparation method of a kind of carnosol according to claim 3 and chitosan composite nano fibre felt, it is characterised in that described lipotropy organic solvent is petroleum ether, benzene, ether, ethanol, dichloromethane, acetone, chloroform, ethyl acetate or normal hexane；Hydrophilic organic solvent is acetone, ethanol, methanol or isopropanol；The lipotropy organic solvent of mixing is at least two in petroleum ether, benzene, ether, ethanol, dichloromethane, acetone, chloroform, ethyl acetate and normal hexane。
5. the preparation method of a kind of carnosol according to claim 1 and chitosan composite nano fibre felt, it is characterised in that in described step (2), the time of stirring is 1～2h；The ultrasonic degassed time is 2～10min。
6. the preparation method of a kind of carnosol according to claim 1 and chitosan composite nano fibre felt, it is characterized in that, in described step (3), the condition of electrostatic spinning is: voltage is 18～35kV, receiving range is 8～22cm, spinning speed is 0.4ml/h, spinneret orifice internal diameter 0.7mm, spinning at room temperature carries out。
7. the preparation method of a kind of carnosol according to claim 1 and chitosan composite nano fibre felt, it is characterised in that in described step (3), the average diameter of nanofiber is 100-700nm。
8. the preparation method of a kind of carnosol according to claim 1 and chitosan composite nano fibre felt, it is characterized in that, in described step (3), the antibiotic rate of escherichia coli and staphylococcus aureus is reached more than 95% by carnosol and chitosan composite nano fibre felt。
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Application publication date: 20160622 |