CN105696194A - Preparation method of carnosol and chitosan self-assembly core sheath composite nanometer fiber mat - Google Patents
Preparation method of carnosol and chitosan self-assembly core sheath composite nanometer fiber mat Download PDFInfo
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- CN105696194A CN105696194A CN201610208031.5A CN201610208031A CN105696194A CN 105696194 A CN105696194 A CN 105696194A CN 201610208031 A CN201610208031 A CN 201610208031A CN 105696194 A CN105696194 A CN 105696194A
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
- C07D493/08—Bridged systems
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0069—Electro-spinning characterised by the electro-spinning apparatus characterised by the spinning section, e.g. capillary tube, protrusion or pin
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0092—Electro-spinning characterised by the electro-spinning apparatus characterised by the electrical field, e.g. combined with a magnetic fields, using biased or alternating fields
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/18—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from other substances
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4382—Stretched reticular film fibres; Composite fibres; Mixed fibres; Ultrafine fibres; Fibres for artificial leather
Abstract
The invention relates to a preparation method of a carnosol and chitosan self-assembly core sheath composite nanometer fiber mat. The method comprises the steps that chitosan is dissolved in a formic acid solution, stirring is conducted, and a chitosan solution is obtained and serves as a sheath fluid; carnosol is extracted from raw materials containing carnosol and dissolved in the formic acid solution, and a carnosol solution is obtained and serves as a core fluid; the chitosan solution is mixed with the carnosol solution, coaxial electrostatic spinning is conducted through a two-channel axial flow injector, and the carnosol and chitosan self-assembly core sheath composite nanometer fiber mat is obtained. The method is simple, easy to carry out and easy to industrialize, and the obtained carnosol and chitosan composite nanometer fiber mat can be used for the fields of wound dressings and the like and is excellent in antibacterial effect and wide in application prospect.
Description
Technical field
The invention belongs to anti-bacterial high-molecule nano-fiber material preparation field, particularly to the preparation method of a kind of carnosol and chitosan self assembly core sheath nanofiber mats。
Background technology
Nanofiber definition have narrow sense, broad sense point。The nanofiber of narrow sense refers to that diameter is nanoscale scope, is namely defined as the fiber that diameter is 1-100nm。As long as the nanofiber of broad sense refers to includes nanostructured in fiber, and impart again new physical property, then can put the category of nanofiber under。Electrostatic spinning is to prepare continuous nano-fibre now, and is expected to the important method of industrialized production。Electrostatic spinning is by several thousand to several ten thousand volt high-pressure electrostatics on polymer solution or melt band, and charged polymer liquid drops in and is accelerated at the Taylor conical point of capillary tube under the active force of electric field。When electric field force is sufficiently large, polymer drop can overcome surface tension to form injection thread。Thread is solvent evaporation or solidification in course of injection, finally falls on the reception device, forms the fiber felt of similar non-woven cloth-like。The fiber prepared with method of electrostatic spinning is much thinner than traditional method, and its diameter is typically between tens nanometer to hundreds of nanometers。
Utilize the fiber felt that nanofiber is made to have significantly high porosity, therefore there is the feature of highly-breathable, add nanofiber and there is big surface area, be commonly used to make medical dressing to promote that wound restores。The microscopic dimensions solving existing medical dressing is general relatively big, when contacting with wound in hemostasis, is unfavorable for the problem absorbing rapidly and suppressing to infect of transudate。The evaporation of wound fluid is conducive to when the medical dressing that nanofiber mats is made is for treatment of wounds, avoid wound surface hydrops, be conducive to oxygen to pass through simultaneously, it is also beneficial to wound tissue Cellular respiration, the growth of epithelial tissue and regeneration, the healing of wound can be dramatically speeded up, by adding antimicrobial component, effectively suppress wound infection further。
Antibacterial used in anti-biotic material can be divided into inorganic antiseptic, organic antibacterial agent, natural antibacterial agent etc.。Wherein with silver ion be representative the chemical property of inorganic antiseptic material own active, be easily reduced into the elemental silver of black when light, heat and ultraviolet, limit its range of application。The use of organic synthesis antibacterial makes vegeto-animal pathogen develop immunity to drugs, and affects health。With the antibacterial being effective ingredient from the plant extract such as Herba Taraxaci, Salvia japonica Thunb., have that source is wide, toxicity is low, not easily cause Drug resistance, nature is prone to degraded, to advantages such as environmental effect are little。Therefore, vegetable active antimicrobial component is increasingly becoming the important channel developing safe efficient antibacterial agent。Wherein phenolic antiseptic can pass through to destroy bacterial cell membrane, makes Cytoplasmic inclusions ooze out, it is suppressed that bacterial dehydrogenase and oxidasic effect, affects the metabolism of antibacterial, thus playing the effect of sterilization。Modern pharmacological research shows, staphylococcus aureus, escherichia coli, bacillus subtilis and Hansenula anomala bacterium are all had inhibitory action in various degree by Salvia japonica Thunb. active ingredient, have broad-spectrum antimicrobial effect。But Salvia japonica Thunb. active ingredient there is also the shortcoming easily losing drug effect under external condition。
Summary of the invention
The technical problem to be solved is to provide a kind of carnosol and the preparation method of chitosan self assembly core sheath nanofiber mats, the method is simple, it is prone to industrialization, gained carnosol composite nanometer fiber felt can be used for the fields such as wound dressing, antibacterial effect is outstanding, and the antibacterial ability persistent period is long。
A kind of carnosol of the present invention and the preparation method of chitosan self assembly core sheath nanofiber mats, including:
(1) chitosan is dissolved in formic acid solution, stirring, obtain the chitosan solution that concentration is 3～5g/ml, as sheath fluid；
(2) from the raw material containing carnosol, extract carnosol, be then dissolved in formic acid solution, obtain carnosol liquid, as core liquid；
(3) chitosan solution in step (1) is mixed with the carnosol solution in step (2), utilize dual pathways axial flow syringe to carry out coaxial electrostatic spinning, obtain carnosol and chitosan self assembly core sheath nanofiber mats；Wherein, chitosan is 8～11:1 with the mass ratio of carnosol。
The mass body volume concentrations 88% of formic acid solution in described step (1) and step (2)。
In described step (2), the extracting method of carnosol is: extracted 2-5 time by the raw material lipotropy organic solvent containing carnosol, united extraction liquid, and concentration obtains the extractum containing carnosol or solid；Dissolving with the hydrophilic organic solvent of 10-90%, filter precipitation, filtrate carries out Macroporous Adsorption Resin process, obtains precipitate containing carnosol；Precipitate dissolves with the lipotropy organic solvent of mixing again, places, and precipitates out precipitation；Filtration precipitates to obtain carnosol, i.e. Salvia japonica Thunb. active antibacterial ingredient。
Described lipotropy organic solvent, refers to that the organic solvent of layering can occur after mixing with water lower than 100 degrees Celsius boiling point, for petroleum ether, benzene, ether, ethanol, dichloromethane, acetone, chloroform, ethyl acetate or normal hexane；Hydrophilic organic solvent, refer to boiling point lower than 100 degrees Celsius, water-miscible organic solvent；For acetone, ethanol, methanol or isopropanol；The lipotropy organic solvent of mixing is at least two in petroleum ether, benzene, ether, ethanol, dichloromethane, acetone, chloroform, ethyl acetate and normal hexane。
Described 10-90% is percent by volume aqueous solution；More superior is 40-70% percent by volume aqueous solution。
Described macroporous adsorbent resin refers to any one in D101, AB-8, HZ818。
In described step (2), the concentration of carnosol solution is 0.3～0.5g/ml。
In described step (3), the condition of coaxial electrostatic spinning is: receiving nano wire with aluminium foil or cloth, voltage is 18～35kV, and receiving range is 10～25cm, and spinning speed is 0.4ml/h, and spinneret orifice internal diameter 0.7mm, spinning at room temperature carries out。
In described step (3), the average diameter of nanofiber is 100-700nm。
In described step (3), the antibiotic rate of escherichia coli and staphylococcus aureus can be reached more than 95% by carnosol and chitosan self assembly core sheath nanofiber mats, and it is lasting to place more than 15 days drug effects。
The present invention utilize continuous print, relatively easy coaxial electrostatic spinning technology (Zhu Limin, Yu Dengguang, Shen Xiaxia, Nie Wei. a kind of Electrospinning coaxial spinning head device。China Patent No.: ZL200920212641.8), obtain carnosol and chitosan self assembly core sheath nanofiber mats。
The present invention obtains being in carnosol and chitosan self assembly core sheath nanofiber mats the Salvia japonica Thunb. active component of sandwich layer and has slow release effect, and duration of efficacy is long。
Beneficial effect
(1) chitosan high polymer used by the present invention, is natural polymer, and the stock number in nature is only second to cellulose, and affinity is good, cheap, less costly；
(2) in the present invention, Salvia japonica Thunb. active component institute expense is few, and preparation is simple, fast, and is positioned among sandwich layer, and the drug effect retention time is long；
(3) present invention prepares in the nanofiber mats of gained that fibre diameter is little, porosity big, and therefore felt specific surface area is big, and antibiotic property is strong；
(4) the method manufacturing process of the present invention is simple, it is easy to large-scale production, has good application and commercial promise。
Accompanying drawing explanation
Fig. 1 is carnosol and the chitosan self assembly core sheath nanofiber mats Electronic Speculum figure of preparation in embodiment 1；
Fig. 2 is the carnosol after placing 15 days in embodiment 1 and chitosan self assembly core sheath nanofiber mats Electronic Speculum figure。
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further。Should be understood that these embodiments are merely to illustrate the present invention rather than restriction the scope of the present invention。In addition, it is to be understood that after having read the content that the present invention lectures, the present invention can be made various changes or modifications by those skilled in the art, and these equivalent form of values fall within the application appended claims limited range equally。
Embodiment 1
Take dry Herba Rosmarini Officinalis branch and leaf 2000g, add dichloromethane 10L, reflux, extract, 3 times, each 3 hours, merge 3 extracting solution, concentration and recovery dichloromethane, obtain extractum；Extractum is dissolved with the ethanol of 60%, is filtered to remove insoluble matter, obtains 1000ml mother solution as adsorption liquid, adsorb with 500mlAB-8 macroporous adsorbent resin, take off by 1000ml purified water through wash after having adsorbed, then with 2000ml80% alcoholic solution eluting, eluent is divided into 6 sections, 4 parts in the middle of merging；The eluent merged is evaporated to 150ml, and add water 200ml, and stirring precipitates out precipitation, places 5 hours；Centrifugal, obtain precipitation containing carnosol；Precipitation 150ml dichloromethane dissolves, and is added thereto to 200ml petroleum ether, and stirring precipitates out precipitation；Filter to obtain carnosol。It is dissolved in formic acid (mass body volume concentrations 88%), obtains the carnosol solution that concentration is 0.4g/ml。
Being dissolved in by 60 grams of chitosans in 15ml formic acid (mass body volume concentrations 88%), magnetic agitation, to being completely dissolved, obtains the chitosan solution that concentration is 4g/ml。
By dual pathways axial flow syringe, electrostatic carnosol/chitosan solution is carried out electrostatic spinning (wherein, the mass ratio of the chitosan in coaxial electrostatic spinning silk device and carnosol is 10:1, ie in solution mass ratio is 1:1), nano wire is received with aluminium foil or cloth, coaxial electrostatic spinning technological parameter is: orifice diameter is 0.7mm, voltage is 25kV, syringe pump fltting speed is 0.2ml/h, employing aluminium foil receives, receiving range is 13cm, under room temperature, spinning obtains carnosol and the chitosan self assembly core sheath nanofiber mats that nanofiber average diameter is 232nm, Fig. 1 passes through the visible core sheath structure nanofiber of scanning electron microscope, this nanofiber places after 15 days structure and unchanged through room temperature, as shown in Figure 2, explanation fibre structure is stable。
Anti-microbial property is evaluated: according to GB/T20944.3-2008, adopting succusion detection by quantitative, with control sample, standby for brand-new and the room temperature placement carnosol of 15 days and chitosan self assembly core sheath nanofiber mats sample are respectively charged into finite concentration, and (viable bacteria number is 3x105In the conical flask of experiment bacterium solution CFU/mL), vibrating 18 hours at 24 DEG C ± 1 DEG C, measure bacterium solution viable bacteria concentration before vibration and after vibration in conical flask, calculate antibiotic rate, evaluate the antibacterial effect of sample with this, evaluation result is in Table 1。
Embodiment 2
Weigh Radix Salviae Miltiorrhizae extract 500g, dissolve with the ethanol of 3000ml50%, be filtered to remove insoluble matter, mother solution adsorbs with 1000mlD101 macroporous adsorbent resin, takes off by 2000ml purified water through wash after having adsorbed, then with 2000ml90% alcoholic solution eluting, eluent is divided into 6 sections, 4 parts in the middle of merging；The eluent merged is evaporated to 500ml, and add water 500ml, and stirring precipitates out precipitation, places 3 hours；Centrifugal, obtain precipitation containing carnosol；Precipitation uses 200ml acetic acid ethyl dissolution, is added thereto to 150ml petroleum ether, stirring, precipitates out precipitation；Filter to obtain carnosol。It is dissolved in formic acid (mass body volume concentrations 88%), obtains the carnosol solution that concentration is 0.5g/ml。
Being dissolved in by 75 grams of chitosans in 15ml formic acid (mass body volume concentrations 88%), magnetic agitation, to being completely dissolved, obtains the chitosan solution that concentration is 5g/ml。
By dual pathways axial flow syringe, electrostatic carnosol/chitosan solution is carried out electrostatic spinning (wherein, the mass ratio of the chitosan in coaxial electrostatic spinning silk device and carnosol is 10:1, ie in solution mass ratio is 1:1), nano wire is received with aluminium foil or cloth, electrostatic spinning process parameter is: orifice diameter is 0.7mm, voltage is 29kV, syringe pump fltting speed is 0.4ml/h, employing aluminium foil receives, receiving range is 16cm, and under room temperature, spinning obtains carnosol and the chitosan self assembly core sheath nanofiber mats that nanofiber average diameter is 412nm。
Anti-microbial property is evaluated: according to GB/T20944.3-2008, adopting succusion detection by quantitative, with control sample, standby for brand-new and the room temperature placement carnosol of 15 days and chitosan self assembly core sheath nanofiber mats sample are respectively charged into finite concentration, and (viable bacteria number is 3x105In the conical flask of experiment bacterium solution CFU/mL), vibrating 18 hours at 24 DEG C ± 1 DEG C temperature, measure bacterium solution viable bacteria concentration before vibration and after vibration in conical flask, calculate antibiotic rate, evaluate the antibacterial effect of sample with this, evaluation result is in Table 1。
Embodiment 3
Take the Salvia japonica Thunb. 2000g of clean dry, add dichloromethane 10L, reflux, extract, 3 times, each 3 hours, merge 3 extracting solution, concentration and recovery dichloromethane, obtain extractum；Extractum is dissolved with the ethanol of 60%, is filtered to remove insoluble matter, obtains 1000ml mother solution as adsorption liquid, adsorb with 500mlAB-8 macroporous adsorbent resin, take off by 1000ml purified water through wash after having adsorbed, then with 2000ml80% alcoholic solution eluting, eluent is divided into 6 sections, 4 parts in the middle of merging；The eluent merged is evaporated to 150ml, and add water 200ml, and stirring precipitates out precipitation, places 5 hours；The precipitation being centrifuged containing carnosol；Precipitation 150ml dichloromethane dissolves, and is added thereto to 200ml petroleum ether, and stirring precipitates out precipitation；Filter to obtain rat-tail active antibacterial composition。It is dissolved in formic acid (mass body volume concentrations 88%), obtains the carnosol solution that concentration is 0.3g/ml；
Being dissolved in by 30 grams of chitosans in 10ml formic acid (mass body volume concentrations 88%), magnetic agitation, to being completely dissolved, obtains the chitosan solution that concentration is 3g/ml。
By dual pathways axial flow syringe, electrostatic carnosol/chitosan solution is carried out electrostatic spinning (wherein, the mass ratio of the chitosan in coaxial electrostatic spinning silk device and carnosol is 10:1, ie in solution mass ratio is 1:1), nano wire is received with aluminium foil or cloth, electrostatic spinning process parameter is: orifice diameter is 0.7mm, voltage is 20kV, syringe pump fltting speed is 0.4ml/h, employing aluminium foil receives, receiving range is 11cm, and under room temperature, spinning obtains carnosol and the chitosan self assembly core sheath nanofiber mats that nanofiber average diameter is 237nm。
Anti-microbial property is evaluated: according to GB/T20944.3-2008, adopting succusion detection by quantitative, with control sample, standby for brand-new and the room temperature placement carnosol of 15 days and chitosan self assembly core sheath nanofiber mats sample are respectively charged into finite concentration, and (viable bacteria number is 3x105In the conical flask of experiment bacterium solution CFU/mL), vibrating 18 hours at 24 DEG C ± 1 DEG C temperature, measure bacterium solution viable bacteria concentration before vibration and after vibration in conical flask, calculate antibiotic rate, evaluate the antibacterial effect of sample with this, evaluation result is in Table 1。
Table 1: the anti-microbial property of carnosol and chitosan self assembly core sheath nanofiber mats
Claims (8)
1. a preparation method for carnosol and chitosan self assembly core sheath nanofiber mats, including:
(1) chitosan is dissolved in formic acid solution, stirring, obtain the chitosan solution that concentration is 3～5g/ml, as sheath fluid；
(2) from the raw material containing carnosol, extract carnosol, be then dissolved in formic acid solution, obtain carnosol solution, as core liquid；
(3) chitosan solution in step (1) is mixed with the carnosol solution in step (2), utilize dual pathways axial flow syringe to carry out coaxial electrostatic spinning, obtain carnosol and chitosan self assembly core sheath nanofiber mats；Wherein, chitosan is 8～11:1 with the mass ratio of carnosol。
2. the preparation method of a kind of carnosol according to claim 1 and chitosan self assembly core sheath nanofiber mats, it is characterised in that described step (1) and the mass body volume concentrations 88% of formic acid solution in step (2)。
3. the preparation method of a kind of carnosol according to claim 1 and chitosan self assembly core sheath nanofiber mats, it is characterized in that, in described step (2), the extracting method of carnosol is: extracted 2-5 time by the raw material lipotropy organic solvent containing carnosol, united extraction liquid, concentration；Dissolving with hydrophilic organic solvent, filter precipitation, filtrate carries out Macroporous Adsorption Resin process, obtains precipitate containing carnosol；Precipitate dissolves with the lipotropy organic solvent of mixing again, places, and precipitates out precipitation；Filtration precipitates to obtain carnosol。
4. the preparation method of a kind of carnosol according to claim 3 and chitosan self assembly core sheath nanofiber mats, it is characterized in that, described lipotropy organic solvent is petroleum ether, benzene, ether, ethanol, dichloromethane, acetone, chloroform, ethyl acetate or normal hexane；Hydrophilic organic solvent is acetone, ethanol, methanol or isopropanol；The lipotropy organic solvent of mixing is at least two in petroleum ether, benzene, ether, ethanol, dichloromethane, acetone, chloroform, ethyl acetate and normal hexane。
5. the preparation method of a kind of carnosol according to claim 1 and chitosan self assembly core sheath nanofiber mats, it is characterised in that in described step (2), the concentration of carnosol solution is 0.3～0.5g/ml。
6. the preparation method of a kind of carnosol according to claim 1 and chitosan self assembly core sheath nanofiber mats, it is characterized in that, in described step (3), the condition of coaxial electrostatic spinning is: receive nano wire with aluminium foil or cloth, voltage is 18～35kV, receiving range is 10～25cm, spinning speed is 0.4ml/h, and spinneret orifice internal diameter 0.7mm, spinning at room temperature carries out。
7. the preparation method of a kind of carnosol according to claim 1 and chitosan self assembly core sheath nanofiber mats, it is characterised in that in described step (3), the average diameter of nanofiber is 100-700nm。
8. the preparation method of a kind of carnosol according to claim 1 and chitosan self assembly core sheath nanofiber mats, it is characterized in that, in described step (3), the antibiotic rate of escherichia coli and staphylococcus aureus can be reached more than 95% by carnosol and chitosan self assembly core sheath nanofiber mats, and it is lasting to place more than 15 days drug effects。
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Application publication date: 20160622 |