CN105676614B - Three visual angle single holographic figure multiple-beam synthesis record system - Google Patents

Three visual angle single holographic figure multiple-beam synthesis record system Download PDF

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Publication number
CN105676614B
CN105676614B CN201610163558.0A CN201610163558A CN105676614B CN 105676614 B CN105676614 B CN 105676614B CN 201610163558 A CN201610163558 A CN 201610163558A CN 105676614 B CN105676614 B CN 105676614B
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light
optical fiber
amici prism
fiber collimator
collimator
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CN105676614A (en
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周文静
张伟
郑财富
吴煜
彭克琴
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University of Shanghai for Science and Technology
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University of Shanghai for Science and Technology
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    • GPHYSICS
    • G03PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03HHOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04Processes or apparatus for producing holograms
    • G03H1/10Processes or apparatus for producing holograms using modulated reference beam
    • G03H1/12Spatial modulation, e.g. ghost imaging
    • GPHYSICS
    • G03PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03HHOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/22Processes or apparatus for obtaining an optical image from holograms

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  • General Physics & Mathematics (AREA)
  • Holo Graphy (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

The present invention provides a kind of three visual angle single holographic figure multiple-beam synthesis record system, including laser, fiber coupler, four optical fiber collimators, three Amici prisms, two reflecting mirrors, optical fiber collimator fixture, article carrying platform, the image receiving system with CCD camera, microscope, computer.Recording device structure of the present invention is simple, easy to operate, convenient for tested sample placement, it can be achieved that transparent sample single width chromatography hologram real-time record, be especially suitable for the real-time acquisition of the living body biological cell of non-preparation.

Description

Three visual angle single holographic figure multiple-beam synthesis record system
Technical field
The present invention relates to a kind of hologram recording recording systems, more particularly to a kind of three visual angle single holographic figure multiple-beam synthesis Record system.
Background technique
Digital hologram chromatographic technique is Digital Holography to be merged with three-dimensional reconstruction algorithm, that is, utilizes Digital Holography The phase information for obtaining testee is realized three-dimensional tomographic reconstruction as the individual-layer data of three-dimensional reconstruction algorithm, be can get Interior of articles tomography refractive index information and three-dimensional structure information.It is aobvious that the technology has had both digital hologram non-intruding, system stabilization etc. Work feature has wide in non-homogeneous transparency material, such as active somatic cell, transparent organism tissue three-dimensional microstructures or refractive index detection General application potential.
Digital hologram chromatographic technique needs to acquire one week phase information of testee, but because being to be directed to transparent substance, So practical only need to acquire the information within the scope of 180 °.There are mainly two types of acquisition methods: 1, the scanning of rotary irradiation light beam is tested Object;2, testee is rotated.Both methods is required to acquire several holograms, so cannot accomplish to acquire in real time.
The device of current three visual angles single holographic figure multiple-beam synthesis record system has no document report.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of three visual angle single holographic figure multiple-beam synthesis to record system, It has the characteristics that structurally reasonable compact, easy to operate.
In order to solve the above-mentioned technical problems, the present invention provides a kind of three visual angle single holographic figure multiple-beam synthesis record systems System, comprising:
Laser issues laser light source;
Fiber coupler receives the laser light source that laser issues and generates the equicohesive light beam of four beams;
Optical fiber collimator, the light beam of reception optical fiber coupler sending are simultaneously converted into directional light;
First reflecting mirror, the carrying object information after light beam irradiation object transmission for issuing the first optical fiber collimator The first object light be reflected on the first Amici prism;
Second reflecting mirror, the carrying object information after light beam irradiation object transmission for issuing third optical fiber collimator Third object light be reflected on the second Amici prism;
First Amici prism, the first object light impinge perpendicularly on the first Amici prism, the second light after the reflection of the first reflecting mirror The light beam of object is irradiated in the sending of fine collimator vertically upward, and the light beam after transmission is the second object light, the first object light and the second object The light beam continuation that light is formed after the transmitting of the first Amici prism reflecting surface is propagated straight up, respectively transmitted through the second light splitting Prism and third Amici prism;
Second Amici prism, third object light impinges perpendicularly on the second Amici prism after the reflection of the second reflecting mirror, by the Continue to propagate straight up after the reflection of two Amici prism reflectings surface;
Third Amici prism receives and impinges perpendicularly on not passed through on third Amici prism by what the 4th optical fiber collimator issued The directional light of any object is crossed as reference light, the reflective surface through third Amici prism, continuation is propagated straight up.
Optical fiber collimator fixture, for fixing optical fiber collimator;
Article carrying platform, for being horizontally arranged biological living cell;
Microscope, for amplifying the picture of the three beams interfering beam collinearly synthesized on third Amici prism;
Image receiving system with CCD camera receives the picture amplified on microscope, and the single width chromatography being amplified is holographic Figure, and it is transmitted to computer;
Computer handles the collected data of the image receiving system with CCD camera.
Preferably, the image receiving system with CCD camera to the record of hologram can be divided into time-sharing recording and Two kinds of situations of record in real time.
Preferably, the clamp base of the optical fiber collimator fixture uses gap structure, mills out one with milling cutter on the base The side of a groove, groove is parallel with the bottom edge of optical fiber collimator fixture, is fixed in bottom using U-lag, realizes to vertical direction Light-beam position fine tuning;And it is equipped with uniform stressed and fixation that threaded hole realizes collimator.
Preferably, the stage design is hollow out mode, and hollow out pore size need to be calculated strictly, size dimension palpus Meet:
One, the spot size of fiber optic collimator light source is 8mm;
Two, testee is independent or the hollow part of article carrying platform is placed on by coverslip, is separated by 60 ° of three visual angle optical fiber Collimated light source intersects at testee center and equal complete illumination testee;
Three, in order to guarantee processing objective table flatness, select objective table with a thickness of 4mm, objective table median pore radius Length must be at least 51.5mm, when actual design, there are surpluses, are set as 55mm.
Preferably, the computer carries out the three visual angle Object light wave information for including in collected single width chromatography hologram Separation, isolated mode are frequency domain separation or time-domain seperation.
Preferably, the plate that the article carrying platform and the optical fiber collimator are placed all is fixed on two dimension and fine-tunes platform On.
Preferably, the three beams object light that the optical fiber collimator issues is in same perpendicular, and mutual angle Intersect at the article carrying platform for placing sample for 60 °.
The positive effect of the present invention is that: recording device structure is simple, easy to operate, convenient for putting for tested sample The real-time record, it can be achieved that the single width chromatography hologram of transparent sample is set, the reality of the living body biological cell of non-preparation is especially suitable for When acquire.The present invention replaces traditional spectroscope to be divided using fiber coupler, is replaced in traditional Fu using optical fiber collimator Leaf collimated optical path, it is final to obtain the equicohesive plane light wave of four beams.Wherein three beams plane light wave is worn with the angle for being separated by 60 ° Object is crossed, by reflecting mirror and spectroscope, last and a branch of not reference light wave Jing Guo any object is interfered in CCD plane, The every light beam of strict guarantee is wanted in whole process to be impinged perpendicularly on the second Amici prism, to guarantee that four bundles light wave can be complete Full incidence CCD forms three visual angle single width and chromatographs off-axis hologram, is passed to computer after CCD is acquired and handled.To holography The record of figure can be divided into time-sharing recording and in real time two kinds of situations of record: (1) time-sharing recording refers in three visual angle single holographic figures In multiple-beam synthesis record system, retain 0 ° or 60 ° or 120 ° of visual angle object lights, while sheltering from other two visual angle object light, according to Three width holograms of secondary record;(2) record refers to that three visual angle object lights synthesize incidence CCD through multiple Amici prisms simultaneously in real time, with ginseng It examines the interference of light and obtains single holographic figure.The equal strength plane light wave that the present invention is issued using fiber coupler and fiber alignment head, The real-time record that three visual angle Object light waves collinearly synthesize, shape are realized in the effect of Amici prism light combination and the adjustment optical path effect of reflecting mirror Hologram is chromatographed at single width.Entire recording device structure is simple and compact, at low cost, does not need rotation testee, may be implemented Real-time dynamically recording is especially suitable for the biological living cell sample detection of non-preparation.
Detailed description of the invention
Fig. 1 is the schematic illustration of three visual angle single holographic figure multiple-beam synthesis record system of the invention;
Fig. 2 is the system apparatus arrangements schematic diagram of three visual angle single holographic figure multiple-beam synthesis record system of the invention;
Fig. 3 is optical fiber collimator fixture entirety installation diagram of the present invention;
Fig. 4 is article carrying platform design diagram of the present invention;
Fig. 5 is article carrying platform three dimensional structure diagram of the present invention.
Specific embodiment
Carry out the embodiment that the present invention will be described in detail below in conjunction with accompanying drawings and embodiments, how the present invention is applied whereby Technological means solves technical problem, and the realization process for reaching technical effect can fully understand and implement.
As depicted in figs. 1 and 2, which mainly includes laser, fiber coupler, four optical fiber collimators, three Amici prism, two reflecting mirrors, optical fiber collimator fixture, article carrying platform, band CCD(Charge-coupled Device, Chinese Full name: charge coupled cell) video camera image receiving system, microscope, computer, in which:
Laser issues laser light source;
Fiber coupler receives the laser light source that laser issues and generates the equicohesive light beam of four beams;
Optical fiber collimator, the light beam of reception optical fiber coupler sending are simultaneously converted into directional light;
First reflecting mirror, the carrying object information after light beam irradiation object transmission for issuing the first optical fiber collimator The first object light be reflected on the first Amici prism;
Second reflecting mirror, the carrying object information after light beam irradiation object transmission for issuing third optical fiber collimator Third object light be reflected on the second Amici prism;
First Amici prism, the first object light impinge perpendicularly on the first Amici prism, the second light after the reflection of the first reflecting mirror The light beam of object is irradiated in the sending of fine collimator vertically upward, and the light beam after transmission is the second object light, the first object light and the second object The light beam continuation that light is formed after the transmitting of the first Amici prism reflecting surface is propagated straight up, respectively transmitted through the second light splitting Prism and third Amici prism;
Second Amici prism, third object light impinges perpendicularly on the second Amici prism after the reflection of the second reflecting mirror, by the Continue to propagate straight up after the reflection of two Amici prism reflectings surface;
Third Amici prism receives and impinges perpendicularly on not passed through on third Amici prism by what the 4th optical fiber collimator issued The directional light of any object is crossed as reference light, the reflective surface through third Amici prism, continuation is propagated straight up.
Optical fiber collimator fixture, for fixing optical fiber collimator;
Article carrying platform, for being horizontally arranged biological living cell;
Microscope, for amplifying the picture of the three beams interfering beam collinearly synthesized on third Amici prism;
Image receiving system with CCD camera receives the picture amplified on microscope, and the single width chromatography being amplified is holographic Figure, and it is transmitted to computer;
Computer handles the collected data of the image receiving system with CCD camera.
The laser light source that laser 1 issues forms the equicohesive light beam of four beams through fiber coupler 2, then respectively by optical fiber Collimator (Fiber Collimator) collimation, obtains four beam directional lights.The first light beam 41 that first optical fiber collimator FC1 is issued Light beam after irradiating object transmission is the first object light O1, carry object information and reflected through the first reflecting mirror M1, impinge perpendicularly on the One Amici prism BS1 forms the continuation of the first light beam 41 after the reflection of the first Amici prism BS1 reflecting surface and passes straight up It broadcasts, respectively transmitted through the second Amici prism BS2, third Amici prism BS3.The second light beam that second optical fiber collimator FC2 is issued 42 irradiate object vertically upward, and the light beam after transmission is the second object light O2, carry object information continuation and propagate straight up, respectively Transmitted through the first Amici prism BS1, the second Amici prism BS2, third Amici prism BS3.What third optical fiber collimator FC3 was issued The 4th light beam 44 that third light beam 43 irradiates after object transmission is third object light O3, it is anti-through the second reflecting mirror M2 to carry object information It penetrates, impinges perpendicularly on the second Amici prism BS2, form the 5th light beam 45 after the reflection of the second Amici prism BS2 reflecting surface Continuation is propagated straight up, transmitted through third Amici prism BS3.The light beam that 4th optical fiber collimator FC4 is issued is not by appointing The directional light of what object directly impinges perpendicularly on the reflective surface of the second Amici prism BS3 as reference light R, continuation vertically to Upper propagation.First object light O of three beams carrying object information1, the second object light O2, third object light O3With reference light R in Amici prism It is collinearly synthesized on the reflecting surface of BS3, realizes the effect of multiple-beam synthesis, collinearly closed in effective image-forming range of microcobjective 3 Light forms three beams interfering beam 46, finally by microcobjective 3 at the picture of amplification, that is, the single width chromatography hologram being amplified, by CCD4 is received.The data of acquisition are transmitted to computer 5 and carry out data processing by CCD4.
The image receiving system with CCD camera can be divided into time-sharing recording and in real time record to the record of hologram Two kinds of situations: one, time-sharing recording refers in three visual angle single holographic figure multiple-beam synthesis record systems, retain 0 ° or 60 ° or 120 ° of visual angle object lights, while other two visual angle object light is sheltered from, successively record three width holograms;Two, record refers to three in real time Visual angle object light synthesizes incidence CCD through multiple Amici prisms simultaneously, obtains single holographic figure with the reference interference of light.
Since the three beams directional light of irradiation testee must be in same plane, and three-beam is separated by stringent 60 °, Specified crosspoint is incident on object, therefore the present invention devises optical fiber collimator fixture, and clamp base uses gap structure, And it is equipped with uniform stressed and fixation that threaded hole realizes collimator.
The present invention is to detect facilitating for sample to place and effectively irradiate, and devises fiber optic collimator light source fixture and loading is flat Platform.
(1) optical fiber collimator fixture
System is recorded by the three visual angle single holographic figure multiple-beam synthesis based on fiber optic collimator light source and multiple Amici prisms Schematic illustration (see attached drawing 1) it is found that the three beams directional light of irradiation testee must be in same plane, and three-beam phase each other Every stringent 60 °, it is incident on object in specified crosspoint, therefore the present invention devises optical fiber collimator as shown in Fig. 5 Fixture, clamp base use gap structure, and are equipped with uniform stressed and fixation that threaded hole realizes collimator.Optical fiber collimator folder It is as shown in Fig. 3 to have whole installation diagram.
(2) article carrying platform
In view of tested sample may relate to biological living cell, three visual angle single holographic figure multiple-beam synthesis of the invention Horizontal positioned article carrying platform is devised in system, and because of the 60 ° of spacing fixed between three visual angle fiber optic collimator light sources, to protect Testee can be completely irradiated to per a branch of light wave by demonstrate,proving, therefore stage design is hollow out mode, and hollow out pore size It need to strictly calculate, as shown in attached drawing 4, Fig. 5.
Hollow out aperture schematic diagram is as shown in Fig. 4 on article carrying platform, and size dimension must meet:
A) spot size of fiber optic collimator light source is 8mm in present system;
B) testee is independent or the hollow part of article carrying platform is placed on by coverslip, is separated by 60 ° of three visual angle optical fiber Collimated light source intersects at testee center and equal complete illumination testee.
C) in order to guarantee processing objective table flatness, select objective table with a thickness of 4mm, objective table median pore radius Length must be at least 51.5mm, when actual design, there are surpluses, are set as 55mm.
Conceive according to above-mentioned invention, the present invention adopts the following technical solutions:
It is a kind of to record system based on three visual angle single holographic figure multiple-beam synthesis of fiber optic collimator light source and multiple Amici prisms System, by a laser light source, a fiber coupler, four fiber alignment heads, three Amici prisms and two reflecting mirrors, one Article carrying platform and corresponding fiber optic collimator fixture head, one with the image receiving system of CCD camera and other mechanical connections Part forms.
Above-mentioned CCD camera connects a computer.
As shown in Figure 4 and Figure 5, it is contemplated that tested sample may relate to biological living cell, three visual angle single width of the invention Horizontal positioned article carrying platform is devised in hologram multiple-beam synthesis system, and because solid between three visual angle fiber optic collimator light sources 60 ° of fixed spacing, to guarantee completely be irradiated to testee per a branch of light wave, therefore stage design is hollow out mould Formula, and hollow out pore size need to be calculated strictly, hollow out aperture schematic diagram is as shown in Fig. 4 on article carrying platform, size dimension palpus Meet:
One, the spot size of fiber optic collimator light source is 8mm in present system;
Two, testee is independent or the hollow part of article carrying platform is placed on by coverslip, is separated by 60 ° of three visual angle optical fiber Collimated light source intersects at testee center and equal complete illumination testee.
Three, in order to guarantee processing objective table flatness, select objective table with a thickness of 4mm, objective table median pore radius Length must be at least 51.5mm, when actual design, there are surpluses, are set as 55mm.
The present invention, which needs collected single width chromatographing the three visual angle Object light wave information for including in hologram, to be separated.Point From mode be frequency domain separation or time-domain seperation.
Frequency domain separation refers to the spectrum distribution for seeking that hologram is chromatographed to single width, and the tilt quantity of reference light wave can be by each to object The spectral carriers of light wave are at different frequency, as long as then extracting point that respective spectrum information realizes three visual angle Object light waves From.By taking single beam Object light wave and record reference light wave interfere superposition on holographic facet as an example, distribution of interference intensity is such as following formula (1):
In formula,WithPhase distribution when being the amplitude and arrival holographic facet of Object light wave respectively,WithIt is respectively The phase distribution when amplitude and arrival holographic facet of reference light wave,It is the coordinate points of hologram plane coordinate system.
According to the frequency shift property of direct current signal and cosine function, Fourier transformation is carried out to above formula, such as following formula (2):
In formula,For the carrier wave item to Object light wave frequency spectrum, the angle between record reference light wave and Object light wave is related. Three visual angle light wave of this system is separated by 60 °, different with the angle of reference light wave, therefore the frequency spectrum of every interference information must Surely it is separated from each other.
Time-domain seperation, which refers to, is reconstructed into respective rebuilding plane three beams Object light wave using different reconstruction distances, realizes each Viewing-angle information separation.Three beams Object light wave is synthetically formed list with different, asymmetrical path and reference beam in present system Width hologram, therefore the recording distance of each visual angle interference information is different from each other.
The purpose of system call interception is to guarantee three beams object light in same perpendicular, and mutual angle is 60 ° intersect at the article carrying platform for placing sample, the system closed with tri- visual angle single holographic figure multiple-beam synthesis of Fig. 2 record system For schematic device, following adjustment has been done to system:
One, guarantee that three beams object light in same vertical plane, and intersects at one by the design of optical fiber collimator pedestal Point.A groove is milled out with milling cutter on the base, the side of groove is parallel with the bottom edge of optical fiber collimator fixture, for limiting optical fiber Collimate the angle of head emergent light.Since there are mismachining tolerances, three-beam, which possibly is present at sample, after installation to intersect, Bottom is fixed using U-lag, is realized and is finely tuned to the light-beam position of vertical direction;
Two, article carrying platform is fixed on two dimension and fine-tunes on platform.Guarantee that loading is flat by adjusting the fine platform of two dimension The horizontality of platform guarantees article carrying platform just in the point of intersection of three beams object light by the height of mobile connection part;
Three, the inclination angle of the first reflecting mirror and the second reflecting mirror is adjusted to guarantee the first object light and third object light strict vertical It is incident on the second Amici prism, and then reflects and continues to propagate straight up;
Four, the plate that the 4th optical fiber collimator is placed is fixed on two dimension and fine-tunes on platform, adjusts the two dimension and finely adjusts The inclination angle of platform is saved to guarantee that reference beam impinges perpendicularly on the second Amici prism;
Five, the focal length for adjusting microcobjective acquires the intensified image of single width chromatography off-axis hologram by CCD.
The purpose of the present invention is obtain three in axially vertical plane using the optical fiber-coupled laser light source with collimating mirror It is fixed to one another the fiber optic collimator light source of 60 ° of spacing while irradiating testee, obtain corresponding three beams object light on three visual angle of object Wave and a branch of reference light wave are synthesized through multiple Amici prisms, are interfered on the photosurface of receiving device CCD, and it is single to form three visual angles Width off-axis hologram.The design is defined as three visual angle single holographic figure multiple-beam synthesis record system.Recording device knot of the present invention Structure is simple, easy to operate, convenient for tested sample placement, it can be achieved that transparent sample single width chromatography hologram real-time record, It is especially suitable for the real-time acquisition of the living body biological cell of non-preparation.
The present invention passes through fiber coupler, the light splitting collimating effect of optical fiber collimator and the special fiber collimator of design Three beams directional light can be irradiated examined object from the different directions for being separated by 60 °, through excessive by fixture and matched article carrying platform The light combination of light prism acts on, and the object light that last three beams carries object information is formed with the reference beam light combination not Jing Guo any object Interference information forms holographic facet in CCD photosurface, and is recorded by CCD and form single width chromatography hologram, strictly protects in whole process Demonstrate,proving every light beam all is impinged perpendicularly on the second Amici prism, final to guarantee multi beam luminous energy incidence CCD completely, to embody mostly light Shu Hecheng.In conjunction with frequency spectrum separation and three-dimensional chromatography algorithm for reconstructing, non-homogeneous transparency material internal structure and characteristic ginseng may be implemented Several detections.Structure of the invention is rationally compact, easy to operate, and system stability is good, adjusts easy and precision height, is suitble to single width layer Analyse the record of hologram.
Particular embodiments described above, the technical issues of to solution of the invention, technical scheme and beneficial effects carry out It is further described, it should be understood that the above is only a specific embodiment of the present invention, is not limited to The present invention, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should be included in this Within the protection scope of invention.

Claims (5)

1. a kind of three visual angle single holographic figure multiple-beam synthesis record system characterized by comprising
Laser issues laser light source;
Fiber coupler receives the laser light source that laser issues and generates the equicohesive light beam of four beams;
Optical fiber collimator, the light beam of reception optical fiber coupler sending are simultaneously converted into directional light;
First reflecting mirror, the of the carrying object information after light beam irradiation object transmission for issuing the first optical fiber collimator One object light is reflected on the first Amici prism;
Second reflecting mirror, the of the carrying object information after light beam irradiation object transmission for issuing third optical fiber collimator Three object lights are reflected on the second Amici prism;
First Amici prism, the first object light impinge perpendicularly on the first Amici prism after the reflection of the first reflecting mirror, and the second optical fiber is quasi- The light beam of object is irradiated in the sending of straight device vertically upward, and the light beam after transmission is the second object light, the first object light and the second object light warp It crosses the light beam continuation formed after the transmitting of the first Amici prism reflecting surface to propagate straight up, respectively transmitted through the second Amici prism With third Amici prism;
Second Amici prism, third object light impinge perpendicularly on the second Amici prism, by second point after the reflection of the second reflecting mirror Continue to propagate straight up after the reflection in light prismatic reflection face;
Third Amici prism receives and impinges perpendicularly on not had by what the 4th optical fiber collimator issued by appointing on third Amici prism The directional light of what object is as reference light, the reflective surface through third Amici prism, and continuation is propagated straight up;
Optical fiber collimator fixture, for fixing optical fiber collimator;The clamp base of the optical fiber collimator fixture uses notch knot Structure mills out a groove with milling cutter on the base, and the side of groove is parallel with the bottom edge of optical fiber collimator fixture, uses U in bottom Shape slot is fixed, and is realized and is finely tuned to the light-beam position of vertical direction, and is equipped with uniform stressed and fixation that threaded hole realizes collimator;
Article carrying platform, for being horizontally arranged biological living cell;
Microscope, for amplifying the picture of the three beams interfering beam collinearly synthesized on third Amici prism;
Image receiving system with CCD camera receives the picture amplified on microscope, and the single width chromatography hologram being amplified, And it is transmitted to computer;
Computer handles the collected data of the image receiving system with CCD camera;
The three beams object light that the optical fiber collimator issues is in same perpendicular, and mutual angle is 60 ° and is placing Intersect at the article carrying platform of sample.
2. single holographic figure multiple-beam synthesis in three visual angles as described in claim 1 records system, which is characterized in that the band The image receiving system of CCD camera can be divided into time-sharing recording and in real time two kinds of situations of record to the record of hologram.
3. single holographic figure multiple-beam synthesis in three visual angles as described in claim 1 records system, which is characterized in that the loading Platform Designing is hollow out mode, and hollow out pore size need to be calculated strictly, and size dimension must meet the following conditions:
One, the spot size of fiber optic collimator light source is 8mm;
Two, testee is independent or the hollow part of article carrying platform is placed on by coverslip, is separated by 60 ° of three visual angle fiber optic collimators Light source intersects at testee center and equal complete illumination testee;
Three, in order to guarantee processing article carrying platform flatness, select article carrying platform with a thickness of 4mm, among article carrying platform The length in aperture must be at least 51.5mm.
4. single holographic figure multiple-beam synthesis in three visual angles as described in claim 1 records system, which is characterized in that the calculating Machine separates the three visual angle Object light wave information for including in collected single width chromatography hologram, and isolated mode is frequency domain point From or time-domain seperation.
5. single holographic figure multiple-beam synthesis in three visual angles as described in claim 1 records system, which is characterized in that the loading The plate that platform and the optical fiber collimator are placed all is fixed on two dimension and fine-tunes on platform.
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