CN105676614A - Three-viewing-angle single-hologram multi-beam combination recording system - Google Patents

Three-viewing-angle single-hologram multi-beam combination recording system Download PDF

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Publication number
CN105676614A
CN105676614A CN201610163558.0A CN201610163558A CN105676614A CN 105676614 A CN105676614 A CN 105676614A CN 201610163558 A CN201610163558 A CN 201610163558A CN 105676614 A CN105676614 A CN 105676614A
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light
splitting prism
fiber optic
beam splitting
optic collimator
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CN201610163558.0A
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CN105676614B (en
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周文静
张伟
郑财富
吴煜
彭克琴
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University of Shanghai for Science and Technology
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University of Shanghai for Science and Technology
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    • GPHYSICS
    • G03PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03HHOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04Processes or apparatus for producing holograms
    • G03H1/10Processes or apparatus for producing holograms using modulated reference beam
    • G03H1/12Spatial modulation, e.g. ghost imaging
    • GPHYSICS
    • G03PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03HHOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/22Processes or apparatus for obtaining an optical image from holograms

Abstract

The invention provides a three-viewing-angle single-hologram multi-beam combination recording system. The recording system comprises a laser, an optical fiber coupler, four optical fiber collimators, three beam splitter prisms, two reflecting mirrors, optical fiber collimator clamps, a bearing platform, an image receiving system equipped with a CCD camera, a microscope and a computer. The recording apparatus is simple in structure, convenient to operate, and convenient to place tested samples; and in addition, the real-time recording of single chromatography hologram of transparent samples can be realized, so that the recording system is quite suitable for real-time sampling of non-prepared living body biological cells.

Description

Three visual angle single width hologram multiple-beam synthesis register systems
Technical field
The present invention relates to a kind of hologram recording recording system, particularly relate to a kind of three visual angle single width hologram multiple-beam synthesis register systems.
Background technology
Digital hologram chromatographic technique is Digital Holography and the fusion of three-dimensional reconstruction algorithm, namely Digital Holography is utilized to obtain the phase place information of testee, it can be used as the individual-layer data of three-dimensional reconstruction algorithm to realize three-dimensional tomographic reconstruction, interior of articles tomography refractive index information and three-dimensional structure information can be obtained. This technology has had the significantly feature such as digital hologram non-intruding, system stability concurrently, at non-homogeneous transparency material, such as the potential that is widely used in the three-dimensional microstructures such as active somatic cell, transparent biological tissue or specific refractory power detection.
Digital hologram chromatographic technique needs to gather the testee phase place information of one week, but because being for transparent substance, so reality only needs the information within the scope of gathering 180 °. Collection method mainly contains two kinds: 1, rotary irradiation beam flying testee; 2, testee is rotated. These two kinds of methods all need to gather several holograms, so real-time collection can not be accomplished.
The device of current three visual angle single width hologram multiple-beam synthesis register systems has no document report.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of three visual angle single width hologram multiple-beam synthesis register systems, and it has feature compact, simple to operate rational in infrastructure.
In order to solve the problems of the technologies described above, the present invention provides a kind of three visual angle single width hologram multiple-beam synthesis register systems, comprising:
Laser apparatus, sends LASER Light Source;
Fiber coupler, the LASER Light Source that reception laser apparatus sends also produces the four equicohesive light beams of bundle;
Fiber optic collimator device, the light beam that reception fiber coupler sends also is converted into parallel light;
First speculum, the first object light carrying object information after the light beam irradiation object transmission sent by the first fiber optic collimator device reflexes to the first beam splitting prism;
Two-mirror, the 3rd object light carrying object information after the light beam irradiation object transmission sent by the 3rd fiber optic collimator device reflexes to the 2nd beam splitting prism;
First beam splitting prism, first object light is after the first speculum reflects, impinge perpendicularly on the first beam splitting prism, the light beam sending irradiating object vertically upward of the 2nd fiber optic collimator device, light beam after transmission is the 2nd object light, the light beam that first object light and the 2nd object light are formed after the transmitting of the first beam splitting prism plane of reflection continues to propagate straight up, respectively transmitted through the 2nd beam splitting prism and the 3rd beam splitting prism;
2nd beam splitting prism, the 3rd object light, after two-mirror reflects, impinges perpendicularly on the 2nd beam splitting prism, continues to propagate straight up after the reflection of the 2nd beam splitting prism plane of reflection;
3rd beam splitting prism, receive impinge perpendicularly on the 3rd beam splitting prism by the 4th fiber optic collimator device send not through the parallel light of any object as with reference to light, the plane of reflection through the 3rd beam splitting prism reflects, continuation is propagated straight up.
Fiber optic collimator device fixture, for fixed fiber collimator;
Carrier table, for horizontal positioned living organisms cell;
Microscope, the three beams for the synthesis that is amplified on the 3rd beam splitting prism conllinear interferes the picture of light beam;
Image receiving system with ccd video camera, receives on microscope the picture amplified, and the single width layer analysis hologram being exaggerated, and transfers to computer;
Computer, the data image receiving system of band ccd video camera collected process.
Preferably, the record of hologram can be divided into time-sharing recording and real time record two kinds of situations by the image receiving system of described band ccd video camera.
Preferably, the clamp base of described fiber optic collimator device fixture adopts gap structure, mills out a groove with milling cutter on pedestal, and the limit of groove is parallel with the base of fiber optic collimator device fixture, U shape groove is adopted to fix in bottom, it is achieved finely tuned by the light-beam position of vertical direction; And be equipped with threaded hole and realize evenly being subject to power and fixing of collimator.
Preferably, described stage design is hollow out pattern, and hollow out pore size needs strict calculating, and its size size must meet:
One, the spot size of fiber optic collimator light source is 8mm;
Two, testee is independent or be placed on the hollow out place of carrier table by cover glass, and the three visual angle fiber optic collimator light sources of 60 ° of being separated by intersect in testee center and equal complete illumination testees;
Three, in order to ensure the planeness of the Stage microscope of processing, the thickness selecting Stage microscope is 4mm, and the length of Stage microscope median pore radius to be at least 51.5mm, during actual design, leaves surplus, is set to 55mm.
Preferably, the three visual angle Object light wave information comprised in the single width layer analysis hologram collected are separated by described computer, and the mode of separation is frequency domain separation or time-domain seperation.
Preferably, the flat board that described carrier table and described fiber optic collimator device are placed all is fixed on two-dimentional meticulous adjustment platform.
Preferably, the three beams object light that described fiber optic collimator device sends is at same perpendicular, and angle each other is that 60 ° of carrier table places at placement sample are crossing.
The positive progressive effect of the present invention is: recording device structure is simple, easy and simple to handle, is convenient to the placement of tested sample, it may be achieved the real time record of the single width layer analysis hologram of transparent sample, is especially applicable to the real-time collection of the living body biological cell of non-preparation. The present invention adopts fiber coupler to replace traditional spectroscope light splitting, utilizes fiber optic collimator device to replace traditional fourier lense collimated light path, the final acquisition four equicohesive plane light waves of bundle. Wherein three beams plane light wave with the angle of 60 ° of being separated by through object, through speculum and spectroscope, finally do not interfere in CCD plane through the reference light wave of any object with a branch of, whole process is wanted the every a branch of light of strict guarantee be all impinge perpendicularly on the 2nd beam splitting prism, to ensure that four bundle light waves can completely incident CCD, form three visual angle single width layers to isolate axle hologram, after CCD gathers, it is passed to computer processes.The record of hologram can be divided into time-sharing recording and real time record two kinds of situations: (1) time-sharing recording refers in three visual angle single width hologram multiple-beam synthesis register systems, retain 0 ° or 60 ° or 120 ° of visual angle object lights, shelter from two other visual angle object light simultaneously, record three width holograms successively; (2) real time record refers to three visual angle object lights simultaneously through the incident CCD of multiple beam splitting prism synthesis, interferes with reference light and obtains single width hologram. The present invention utilizes the equal strength plane light wave that fiber coupler and fiber optic collimator hair go out, and the adjustment light path effect that beam splitting prism closes light action and speculum realizes the real time record of three visual angle Object light wave conllinear synthesis, forms single width layer analysis hologram. Whole recording device structure is simply compact, and cost is low, it is not necessary to rotate testee, it is possible to realize Real-time and Dynamic record, is especially applicable to the living organisms cell sample detection of non-preparation.
Accompanying drawing explanation
Fig. 1 is the principle schematic of the present invention three visual angle single width hologram multiple-beam synthesis register system;
Fig. 2 is the system apparatus arrangements schematic diagram of the present invention three visual angle single width hologram multiple-beam synthesis register system;
Fig. 3 is fiber optic collimator device fixture integral installation figure of the present invention;
Fig. 4 is carrier table design diagram of the present invention;
Fig. 5 is carrier table three-dimensional structure schematic diagram of the present invention.
Embodiment
Below in conjunction with drawings and Examples, embodiments of the present invention being described in detail, to the present invention, how utilisation technology means carry out technical solution problem whereby, and the process that realizes reaching technique effect can fully understand and implement according to this.
As depicted in figs. 1 and 2, this embodiment mainly comprises laser apparatus, fiber coupler, four fiber optic collimator devices, three beam splitting prisms, two speculums, fiber optic collimator device fixture, carrier table, band CCD(Charge-coupledDevice, Chinese full name: charge coupled cell) image receiving system of pick up camera, microscope, computer, wherein:
Laser apparatus, sends LASER Light Source;
Fiber coupler, the LASER Light Source that reception laser apparatus sends also produces the four equicohesive light beams of bundle;
Fiber optic collimator device, the light beam that reception fiber coupler sends also is converted into parallel light;
First speculum, the first object light carrying object information after the light beam irradiation object transmission sent by the first fiber optic collimator device reflexes to the first beam splitting prism;
Two-mirror, the 3rd object light carrying object information after the light beam irradiation object transmission sent by the 3rd fiber optic collimator device reflexes to the 2nd beam splitting prism;
First beam splitting prism, first object light is after the first speculum reflects, impinge perpendicularly on the first beam splitting prism, the light beam sending irradiating object vertically upward of the 2nd fiber optic collimator device, light beam after transmission is the 2nd object light, the light beam that first object light and the 2nd object light are formed after the transmitting of the first beam splitting prism plane of reflection continues to propagate straight up, respectively transmitted through the 2nd beam splitting prism and the 3rd beam splitting prism;
2nd beam splitting prism, the 3rd object light, after two-mirror reflects, impinges perpendicularly on the 2nd beam splitting prism, continues to propagate straight up after the reflection of the 2nd beam splitting prism plane of reflection;
3rd beam splitting prism, receive impinge perpendicularly on the 3rd beam splitting prism by the 4th fiber optic collimator device send not through the parallel light of any object as with reference to light, the plane of reflection through the 3rd beam splitting prism reflects, continuation is propagated straight up.
Fiber optic collimator device fixture, for fixed fiber collimator;
Carrier table, for horizontal positioned living organisms cell;
Microscope, the three beams for the synthesis that is amplified on the 3rd beam splitting prism conllinear interferes the picture of light beam;
Image receiving system with ccd video camera, receives on microscope the picture amplified, and the single width layer analysis hologram being exaggerated, and transfers to computer;
Computer, the data image receiving system of band ccd video camera collected process.
The LASER Light Source that laser apparatus 1 sends forms the four equicohesive light beams of bundle through fiber coupler 2, then respectively by fiber optic collimator device (FiberCollimator) collimation, obtains the four parallel light of bundle. Light beam after the first light beam 41 irradiating object transmission that first fiber optic collimator device FC1 sends is the first object light O1Carry object information to reflect through the first mirror M 1, impinge perpendicularly on the first beam splitting prism BS1, after the reflection of the first beam splitting prism BS1 plane of reflection, form the first light beam 41 continue to propagate straight up, respectively transmitted through the 2nd beam splitting prism BS2, the 3rd beam splitting prism BS3. The 2nd light beam 42 irradiating object vertically upward that 2nd fiber optic collimator device FC2 sends, the light beam after transmission is the 2nd object light O2, carry object information and continue to propagate straight up, respectively transmitted through the first beam splitting prism BS1, the 2nd beam splitting prism BS2, the 3rd beam splitting prism BS3. The 4th light beam 44 after the 3rd light beam 43 irradiating object transmission that 3rd fiber optic collimator device FC3 sends is the 3rd object light O3, carry object information and reflect through two-mirror M2, impinge perpendicularly on the 2nd beam splitting prism BS2, after the reflection of the 2nd beam splitting prism BS2 plane of reflection, form the 5th light beam 45 continue to propagate straight up, transmitted through the 3rd beam splitting prism BS3. The light beam that 4th fiber optic collimator device FC4 sends is the plane of reflection reflection directly not impinging perpendicularly on the 2nd beam splitting prism BS3 through the parallel light of any object as reference light R, continues to propagate straight up. Three beams carries the first object light O of object information1, the 2nd object light O2, the 3rd object light O3With reference light R conllinear synthesis on the plane of reflection of beam splitting prism BS3, achieve the effect of multiple-beam synthesis, effective image-forming range conllinear at microcobjective 3 closes light and forms three beams interference light beam 46, eventually pass the picture that microcobjective 3 one-tenth amplifies, namely the single width layer analysis hologram being exaggerated, is received by CCD4. The transfer of collection is carried out data processing to computer 5 by CCD4.
The record of hologram can be divided into time-sharing recording and real time record two kinds of situations by the image receiving system of described band ccd video camera: one, time-sharing recording refers in three visual angle single width hologram multiple-beam synthesis register systems, retain 0 ° or 60 ° or 120 ° of visual angle object lights, shelter from two other visual angle object light simultaneously, record three width holograms successively; Two, real time record refers to three visual angle object lights simultaneously through the incident CCD of multiple beam splitting prism synthesis, interferes with reference light and obtains single width hologram.
Must in same plane owing to irradiating the parallel light of three beams of testee, and three-beam is separated by strict 60 °, incide on object in the joining specified, therefore inventive design fiber optic collimator device fixture, clamp base adopts gap structure, and is equipped with threaded hole and realizes evenly being subject to power and fixing of collimator.
The present invention is the convenient placement of detection sample and effectively irradiates, and devises fiber optic collimator light source fixture and carrier table.
(1) fiber optic collimator device fixture
From the principle schematic (see accompanying drawing 1) of the three visual angle single width hologram multiple-beam synthesis register systems based on fiber optic collimator light source and multiple beam splitting prism, the parallel light of three beams irradiating testee must in same plane, and three-beam is separated by strict 60 °, incide on object in the joining specified, therefore inventive design fiber optic collimator device fixture as shown in Figure 5, clamp base adopts gap structure, and is equipped with threaded hole and realizes evenly being subject to power and fixing of collimator.Fiber optic collimator device fixture integral installation figure is as shown in Figure 3.
(2) carrier table
Consider that tested sample may relate to living organisms cell, the present invention three visual angle single width hologram multiple-beam synthesis system devises the carrier table of horizontal positioned, again because of 60 ° of spacing fixing between three visual angle fiber optic collimator light sources, for ensureing that every a branch of light ware energy is enough intactly irradiated to testee, therefore stage design is hollow out pattern, and hollow out pore size needs strict calculating, as shown in accompanying drawing 4, Fig. 5.
On carrier table, hollow out aperture schematic diagram is as shown in Figure 4, and its size size must meet:
A) in system of the present invention, the spot size of fiber optic collimator light source is 8mm;
B) testee is independent or be placed on the hollow out place of carrier table by cover glass, and the three visual angle fiber optic collimator light sources of 60 ° of being separated by intersect in testee center and equal complete illumination testees.
C) in order to ensure the planeness of the Stage microscope of processing, the thickness selecting Stage microscope is 4mm, and the length of Stage microscope median pore radius to be at least 51.5mm, during actual design, leaves surplus, is set to 55mm.
According to above-mentioned invention design, the present invention adopts following technical proposals:
Based on three visual angle single width hologram multiple-beam synthesis register systems of fiber optic collimator light source and multiple beam splitting prism, it is made up of the image receiving system of a LASER Light Source, a fiber coupler, four fiber optic collimator heads, three beam splitting prisms and two speculums, a carrier table and corresponding fiber optic collimator fixture head, a band ccd video camera and other mechanical connecting part.
Above-mentioned ccd video camera connects a computer.
As shown in Figure 4 and Figure 5, consider that tested sample may relate to living organisms cell, the present invention three visual angle single width hologram multiple-beam synthesis system devises the carrier table of horizontal positioned, again because of 60 ° of spacing fixing between three visual angle fiber optic collimator light sources, for ensureing that every a branch of light ware energy is enough intactly irradiated to testee, therefore stage design is hollow out pattern, and hollow out pore size needs strict calculating, on carrier table, hollow out aperture schematic diagram is as shown in Figure 4, and its size size must meet:
One, in system of the present invention, the spot size of fiber optic collimator light source is 8mm;
Two, testee is independent or be placed on the hollow out place of carrier table by cover glass, and the three visual angle fiber optic collimator light sources of 60 ° of being separated by intersect in testee center and equal complete illumination testees.
Three, in order to ensure the planeness of the Stage microscope of processing, the thickness selecting Stage microscope is 4mm, and the length of Stage microscope median pore radius to be at least 51.5mm, during actual design, leaves surplus, is set to 55mm.
The present invention needs that the single width layer collected is analysed in hologram the three visual angle Object light wave information comprised and is separated. The mode of separation is frequency domain separation or time-domain seperation.
Frequency domain separation refer to ask for single width layer analyse hologram spectrum distribution, the tilt quantity of reference light wave can by each to the spectral carriers of Object light wave to difference frequency place, as long as then extracting the separation that respective spectrum information just can realize three visual angle Object light wave. Interfering on holographic facet with single bundle Object light wave and record reference light wave and repeatedly add as example, its distribution of interference intensity is as shown in the formula (1):
In formula,WithIt is phase distribution during amplitude and the arrival holographic facet of Object light wave respectively,WithIt is phase distribution during amplitude and the arrival holographic facet of reference light wave respectively,It it is the coordinate point of hologram plane system of coordinates.
According to the frequency displacement characteristic of direct current signal and cosine function, upper formula is carried out fourier transformation, as shown in the formula (2):
In formula,For the carrier wave item to Object light wave frequency spectrum, relevant to the angle between record reference light wave and Object light wave. Native system three visual angle light wave is separated by 60 °, and different with the angle of reference light wave, therefore the frequency spectrum of every interference information must be separated from each other.
Time-domain seperation refers to and utilizes different reconstruction distances that three beams Object light wave is reconstructed into respective rebuilding plane, it is achieved each Viewing-angle information is separated. In system of the present invention, three beams Object light wave forms single width hologram with different, asymmetric path and reference beam synthesis, and therefore the recording distance of each visual angle interference information is different from each other.
The object of system call interception is to ensure that three beams object light is at same perpendicular, and angle each other is 60 ° to intersect at the carrier table place placing sample, for the system and device schematic diagram that Fig. 2 tri-visual angle single width hologram multiple-beam synthesis register system is closed, system is done following adjustment:
One, ensure that three beams object light is in same vertical plane by the design of fiber optic collimator device pedestal, and intersect at a point. Milling out a groove with milling cutter on pedestal, the limit of groove is parallel with the base of fiber optic collimator device fixture, be used for limit fibre collimation head emergent light angle. Owing to there is processing error, after installation, three-beam may appear at sample place and can not intersect, and adopts U shape groove to fix in bottom, it is achieved finely tuned by the light-beam position of vertical direction;
Two, carrier table is fixed on two-dimentional meticulous adjustment platform. By regulating two-dimentional meticulous platform to ensure the horizontality of carrier table, ensure carrier table just in the point of intersection of three beams object light by the height of mobile link part;
Three, regulate the inclination angle of the first speculum and two-mirror ensure the first object light and the 3rd object light strict impinge perpendicularly on the 2nd beam splitting prism, and then reflection continue propagate straight up;
Four, the flat board that the 4th fiber optic collimator device is placed is fixed on two-dimentional meticulous adjustment platform, and reference beam impinges perpendicularly on the 2nd beam splitting prism to ensure to regulate the inclination angle of this two-dimentional meticulous adjustment platform;
Five, adjust the focal length of microcobjective, the isolate amplification picture of axle hologram of single width layer is gathered by CCD.
It is an object of the invention to utilize the optical fiber-coupled laser light source of band collimation mirror to obtain three fiber optic collimator light sources being fixed to one another 60 ° of spacing in axially vertical plane and irradiate testee simultaneously, obtain corresponding three beams Object light wave on object three visual angle, synthesize through multiple beam splitting prism with a branch of reference light wave, the photosensitive surface receiving device CCD is interfered, forms three visual angle single width off-axis gaussian beam figure. The design is defined as three visual angle single width hologram multiple-beam synthesis register systems. Recording device structure of the present invention is simple, easy and simple to handle, is convenient to the placement of tested sample, it may be achieved the real time record of the single width layer analysis hologram of transparent sample, is especially applicable to the real-time collection of the living body biological cell of non-preparation.
The present invention passes through fiber coupler, the light splitting collimation effect of fiber optic collimator device and the special fiber collimator fixture of design and supporting carrier table, can by parallel for three beams light from be separated by 60 ° different directions irradiate examined object, through the conjunction light action of beam splitting prism, last three beams is carried the object light of object information and is not closed light formation interference information through the reference beam of any object, holographic facet is formed at CCD photosensitive surface, and form single width layer analysis hologram by CCD record, in whole process, the every a branch of light of strict guarantee is all impinge perpendicularly on the 2nd beam splitting prism, the final completely incident CCD of guarantee multi-beam energy, to embody multiple-beam synthesis.In conjunction with frequency spectrum separation and three-dimension layer analysis reconstruction algorithm, it is possible to realize the detection of non-homogeneous transparency material internal structure and characteristic parameter. Present configuration is rationally compact, easy and simple to handle, and system stability is good, regulates easily and precision height, is applicable to the record of single width layer analysis hologram.
Above-described specific embodiment; the technical problem of the solution of the present invention, technical scheme and useful effect have been further described; it is it should be understood that; the foregoing is only specific embodiments of the invention; it is not limited to the present invention; within the spirit and principles in the present invention all, any amendment of making, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (7)

1. a visual angle single width hologram multiple-beam synthesis register system, it is characterised in that, comprising:
Laser apparatus, sends LASER Light Source;
Fiber coupler, the LASER Light Source that reception laser apparatus sends also produces the four equicohesive light beams of bundle;
Fiber optic collimator device, the light beam that reception fiber coupler sends also is converted into parallel light;
First speculum, the first object light carrying object information after the light beam irradiation object transmission sent by the first fiber optic collimator device reflexes to the first beam splitting prism;
Two-mirror, the 3rd object light carrying object information after the light beam irradiation object transmission sent by the 3rd fiber optic collimator device reflexes to the 2nd beam splitting prism;
First beam splitting prism, first object light is after the first speculum reflects, impinge perpendicularly on the first beam splitting prism, the light beam sending irradiating object vertically upward of the 2nd fiber optic collimator device, light beam after transmission is the 2nd object light, the light beam that first object light and the 2nd object light are formed after the transmitting of the first beam splitting prism plane of reflection continues to propagate straight up, respectively transmitted through the 2nd beam splitting prism and the 3rd beam splitting prism;
2nd beam splitting prism, the 3rd object light, after two-mirror reflects, impinges perpendicularly on the 2nd beam splitting prism, continues to propagate straight up after the reflection of the 2nd beam splitting prism plane of reflection;
3rd beam splitting prism, receive impinge perpendicularly on the 3rd beam splitting prism by the 4th fiber optic collimator device send not through the parallel light of any object as with reference to light, the plane of reflection through the 3rd beam splitting prism reflects, continuation is propagated straight up;
Fiber optic collimator device fixture, for fixed fiber collimator;
Carrier table, for horizontal positioned living organisms cell;
Microscope, the three beams for the synthesis that is amplified on the 3rd beam splitting prism conllinear interferes the picture of light beam;
Image receiving system with ccd video camera, receives on microscope the picture amplified, and the single width layer analysis hologram being exaggerated, and transfers to computer;
Computer, the data image receiving system of band ccd video camera collected process.
2. three visual angle single width hologram multiple-beam synthesis register systems as claimed in claim 1, it is characterised in that, the record of hologram can be divided into time-sharing recording and real time record two kinds of situations by the image receiving system of described band ccd video camera.
3. three visual angle single width hologram multiple-beam synthesis register systems as claimed in claim 1, it is characterized in that, the clamp base of described fiber optic collimator device fixture adopts gap structure, pedestal mills out a groove with milling cutter, the limit of groove is parallel with the base of fiber optic collimator device fixture, adopt in bottom U shape groove to fix, it is achieved to be finely tuned by the light-beam position of vertical direction, and it is equipped with threaded hole and realizes evenly being subject to power and fixing of collimator.
4. three visual angle single width hologram multiple-beam synthesis register systems as claimed in claim 1, it is characterised in that, described stage design is hollow out pattern, and hollow out pore size needs strict calculating, and its size size must meet the following conditions:
One, the spot size of fiber optic collimator light source is 8mm;
Two, testee is independent or is placed on the hollow out place of carrier table by cover glass, and the three visual angle fiber optic collimator light sources of 60 ° of being separated by intersect in testee center and equal complete illumination testees;
Three, in order to ensure the planeness of the Stage microscope of processing, the thickness selecting Stage microscope is 4mm, and the length of Stage microscope median pore radius to be at least 51.5mm, during actual design, leaves surplus, is set to 55mm.
5. three visual angle single width hologram multiple-beam synthesis register systems as claimed in claim 4, it is characterized in that, the three visual angle Object light wave information comprised in the single width layer analysis hologram collected are separated by described computer, and the mode of separation is frequency domain separation or time-domain seperation.
6. three visual angle single width hologram multiple-beam synthesis register systems as claimed in claim 1, it is characterised in that, the flat board that described carrier table and described fiber optic collimator device are placed all is fixed on two-dimentional meticulous adjustment platform.
7. three visual angle single width hologram multiple-beam synthesis register systems as claimed in claim 1, it is characterized in that, the three beams object light that described fiber optic collimator device sends is at same perpendicular, and angle each other is that 60 ° of carrier table places at placement sample are crossing.
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Cited By (4)

* Cited by examiner, † Cited by third party
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CN106527093A (en) * 2016-12-26 2017-03-22 北京理工大学 Nonlinear frequency doubling and polarization characteristic-based hologram multiplexing method and system
CN106767469A (en) * 2016-11-29 2017-05-31 四川凌峰航空液压机械有限公司 Close as the non-contact measurement method in measurement Fine and Deep Hole class aperture
CN107478173A (en) * 2017-06-26 2017-12-15 合肥工业大学 A kind of particulate samples pond and microparticle particle shape coaxial digital holography measurement apparatus
CN110887813A (en) * 2019-11-21 2020-03-17 上海大学 Method for realizing microstructure detection of functionally graded material based on digital acousto-optic holography

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090286165A1 (en) * 2008-05-16 2009-11-19 Sony Corporation Hologram laminate, hologram copy method, and hologram producing method
JP2009277272A (en) * 2008-05-13 2009-11-26 Canon Inc Hologram recording and reproducing device
CN102231055A (en) * 2011-06-30 2011-11-02 上海大学 Tricolor recording layered reproduced dynamic hologram recording device
CN102290060A (en) * 2011-06-24 2011-12-21 上海大学 Axonometric single chromatography hologram recording device
CN104049516A (en) * 2014-06-17 2014-09-17 中国石油大学(华东) True color phase-shifting digital holographic object light reconstructing and chromatic aberration correcting method
CN104159094A (en) * 2014-07-09 2014-11-19 四川大学 Method for improving optical scanning holographic tomography effect
CN104390722A (en) * 2014-11-14 2015-03-04 北京航空航天大学 Optical fiber conduction-based digital holographic tomographic three-dimensional temperature measuring device
CN104834201A (en) * 2015-05-13 2015-08-12 北京工业大学 Dual-wavelength polarization multiplexing digital holographic imaging system and method

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009277272A (en) * 2008-05-13 2009-11-26 Canon Inc Hologram recording and reproducing device
US20090286165A1 (en) * 2008-05-16 2009-11-19 Sony Corporation Hologram laminate, hologram copy method, and hologram producing method
CN102290060A (en) * 2011-06-24 2011-12-21 上海大学 Axonometric single chromatography hologram recording device
CN102231055A (en) * 2011-06-30 2011-11-02 上海大学 Tricolor recording layered reproduced dynamic hologram recording device
CN104049516A (en) * 2014-06-17 2014-09-17 中国石油大学(华东) True color phase-shifting digital holographic object light reconstructing and chromatic aberration correcting method
CN104159094A (en) * 2014-07-09 2014-11-19 四川大学 Method for improving optical scanning holographic tomography effect
CN104390722A (en) * 2014-11-14 2015-03-04 北京航空航天大学 Optical fiber conduction-based digital holographic tomographic three-dimensional temperature measuring device
CN104834201A (en) * 2015-05-13 2015-08-12 北京工业大学 Dual-wavelength polarization multiplexing digital holographic imaging system and method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106767469A (en) * 2016-11-29 2017-05-31 四川凌峰航空液压机械有限公司 Close as the non-contact measurement method in measurement Fine and Deep Hole class aperture
CN106527093A (en) * 2016-12-26 2017-03-22 北京理工大学 Nonlinear frequency doubling and polarization characteristic-based hologram multiplexing method and system
CN106527093B (en) * 2016-12-26 2019-03-19 北京理工大学 Hologram image multiplexing method and system based on frequency doubling non-linear and polarization characteristic
CN107478173A (en) * 2017-06-26 2017-12-15 合肥工业大学 A kind of particulate samples pond and microparticle particle shape coaxial digital holography measurement apparatus
CN110887813A (en) * 2019-11-21 2020-03-17 上海大学 Method for realizing microstructure detection of functionally graded material based on digital acousto-optic holography
CN110887813B (en) * 2019-11-21 2022-04-12 上海大学 Method for realizing microstructure detection of functionally graded material based on digital acousto-optic holography

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