CN105664048A

CN105664048A - Pharmaceutical composition inhibiting gastric cancer cell proliferation and application thereof

Info

Publication number: CN105664048A
Application number: CN201610124941.5A
Authority: CN
Inventors: 马晓莹
Original assignee: Qingdao Municipal Hospital
Current assignee: Qingdao Municipal Hospital
Priority date: 2016-03-04
Filing date: 2016-03-04
Publication date: 2016-06-15

Abstract

The invention discloses a pharmaceutical composition inhibiting gastric cancer cell proliferation. The pharmaceutical composition consists of the following active pharmaceutical ingredients: liquorice, herba agrimoniae, pseudo-ginseng, sappan wood, rhizoma acori tatarinowii, houttuynia cordata, white mulberry root-bark, coptis chinensis, curcuma zedoary, rhizoma cyperi, radix gentianae macrophyllae, radix ophiopogonis, fructus aurantii, radix aucklandiae, kadsura longepedunculata, cardamun, radix ilicis asprellae, isodon amethystoides, lalang grass rhizome, sculellaria barbata, hedyotis diffusa, rhizoma polygoni cuspidati, wideleaf osbeckia root, poria cocos, scutellaria baicalensis, rhizoma sparganii, fructus gardeniae, fiveleaf gynostemma herb, radix puerariae, ligusticum wallichii and asarum sieboldii. The compatibility of the pharmaceutical composition conforms to the principle of 'monarch, minister, assistant and guide' of traditional Chinese medicines, gives prominence to the key points and is comprehensive and reasonable; the pharmaceutical composition has the advantages of strong effect and quick and stable efficacy, realizes the functions of clearing heat and removing toxin and activating blood and resolving stasis and promises a good effect of inhibiting gastric cancer cell proliferation; and moreover, the pharmaceutical composition has the characteristics of rare relapse, no side effect and the like.

Description

A kind of pharmaceutical composition suppressing proliferation of human gastric cancer cell and application thereof

Technical field

The invention belongs to pharmaceutical technology field, relate to a kind of Chinese medicine composition, be specifically related to a kind of pharmaceutical composition suppressing proliferation of human gastric cancer cell and application thereof.

Background technology

The health of we mankind is made up of upper trillion cells, these cells are the basic function unit of organization of human body, the health of cell is under the regulation and control of nucleic acid, relies on protein, several amino acids, polypeptide, polysaccharide, various trace elements, mineral etc. with bioactive material to maintain the homergy of cell. Cell has specific responsibility, collaborative work, remains our healthy. Each tissue in health is by cellularity, and relevant cell tissue cluster just constitutes the organ of human body. Although these organs are by the different cellularity of function, but, they need same basic nutrition composition, and this is the basic substance supporting cellular metabolism. Cell has the life-span, in theory, if providing suitable, enough basic nutrition composition for cell, eliminate the cell of apoptosis, the healthy cell that accurate duplication has existed, improves the human body self-healing ability for disease, our mankind's delaying sanility, keeps fit.

Human body is for the self-healing ability of disease, namely it is the mankind's inherent vitalitys of relying on self, relying on immune system, nervous system and hormonal system is main repair mechanism, repairs impaired cell, throw off one's illness a kind of ability kept fit with sub-health state. When the vis medicatrix naturae of human body declines, being because some human organ tissue system and lack enough trophic factors, cell can not normally metabolism, it is impossible to completely correctly replicate healthy cell and the cell of liquefaction apoptosis. Incorrect duplication due to cell so that the normal function of cell is made a discount, the self-repairing capability of health lowers, and this directly affects the human body resistivity for disease, once meet with extraneous undesirable element, human body will be sick.

Cancer has been the first big persistent ailment of the mankind now, the feature of cancerous cell be unrestrictedly, hypertrophy without end, make nutrient substance in the patient be consumed in a large number; Cancerous cell discharges multiple toxin, makes human body produce series of symptoms; Cancerous cell also can be transferred to whole body growth and breeding everywhere, cause human body to be become thin, unable, anemia, inappetence, heating and serious organ function impaired etc.

Gastric cancer is one of modal malignant tumor of China, the first place of all kinds of tumor is occupied at its sickness rate of China, there are about 170,000 people every year and die from gastric cancer, almost close to the 1/4 of whole mortality of malignant tumors numbers, and annual also having more than 20,000 new Patients with Gastric Cancer to generate, gastric cancer is strictly the disease of a kind of serious threat people's health. Gastric cancer can betide any age, but common with 40～60 years old, and man, more than female, is about 2: 1. Gastric cancer can betide any position of stomach, but is more common in gastric antrum portion, especially lesser gastric curvature side. Untreated person's average life is about 13 months.

The drug main treating gastric cancer clinically at present to have chemical drugs, but owing to chemotherapeutics toxicity is big, while killing tumor cell also non-selectivity kill human autoimmune's cell such as leukocyte, lymphocyte, cause patient usually due to because of autoimmune inferior capabilities accompanying infection, patient uses erious adverse reaction, and the course for the treatment of is long, costly, Clinical practice has bigger limitation.

Along with the development of medical skill, motherland's traditional medicine obtains the favor of more and more patients with gastric cancer. Traditional Chinese Medicine for Gastric Carcinoma is mainly reflected in and improves precancerous lesion, postoperative righting auxiliary treatment and alleviate these 3 aspects of toxic and side effects of chemotherapy. Primary treatments includes Chinese medicine for oral administration compound decoction, powder, capsule, ball dissipate, external fumigation and washing method, application, rinsing the mouth method, clysmata, the method such as foot bath method. At present, the effect mechanism utilizing modern molecular biology technique research Traditional Chinese Medicine for Gastric Carcinoma becomes the focus of research, is mainly reflected in: cytotoxicity, on the impact of apoptosis of tumor cells, suppress tumor invasion and metabasis, suppress the several respects such as tumor-blood-vessel growth.

Summary of the invention

It is an object of the invention to provide a kind of pharmaceutical composition suppressing proliferation of human gastric cancer cell and application thereof, this pharmaceutical composition technique is simple, and cost is low, efficiency is high, has no side effect, and has effect that clear skin ulcer is sterilized, ridging consolidates, can effectively suppress proliferation of human gastric cancer cell, bring Gospel to patients with gastric cancer.

The above-mentioned purpose of the present invention is realized by following technical proposals:

A kind of pharmaceutical composition suppressing proliferation of human gastric cancer cell, the crude drug of described pharmaceutical composition is made up of Radix Glycyrrhizae, Herba Agrimoniae, Radix Notoginseng, Lignum Sappan, Rhizoma Acori Graminei, Herba Houttuyniae, Cortex Mori, Rhizoma Coptidis, Rhizoma Curcumae, Rhizoma Cyperi, Radix Gentianae Macrophyllae, Radix Ophiopogonis, Fructus Aurantii, the Radix Aucklandiae, Fructus Schisandrae Sphenantherae, Fructus Amomi Rotundus, Flos Ilicis Asprellae, Herba Rabdosiae glaucocalycis, Rhizoma Imperatae, Herba Scutellariae Barbatae, Herba Hedyotidis Diffusae, Rhizoma Polygoni Cuspidati, Radix Osbeckiae Crinitae, Poria, Radix Scutellariae, Rhizoma Sparganii, Fructus Gardeniae, Herb Gynostemmae Pentaphylli, Radix Puerariae, Rhizoma Chuanxiong, Herba Asari.

In a preferred embodiment, make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 12-20 part, Radix Scutellariae 10-16 part, Rhizoma Imperatae 3-8 part, Radix Glycyrrhizae 5-12 part, Radix Notoginseng 12-17 part, Herba Agrimoniae 7-12 part, Fructus Amomi Rotundus 4-9 part, Radix Ophiopogonis 15-20 part, Rhizoma Chuanxiong 10-15 part, Radix Gentianae Macrophyllae 8-12 part, Fructus Aurantii 2-5 part, Rhizoma Acori Graminei 3-7 part, Fructus Schisandrae Sphenantherae 11-14 part, Herba Houttuyniae 9-15 part, Herba Scutellariae Barbatae 3-6 part, Rhizoma Cyperi 8-13 part, Herba Rabdosiae glaucocalycis 3-5 part, Cortex Mori 1-3 part, Herba Hedyotidis Diffusae 5-10 part, Rhizoma Coptidis 5-10 part, Rhizoma Polygoni Cuspidati 13-16 part, Lignum Sappan 3-10 part, Radix Osbeckiae Crinitae 8-13 part, Rhizoma Sparganii 3-5 part, Flos Ilicis Asprellae 1-3 part, Radix Puerariae 5-10 part, Poria 3-6 part, Radix Aucklandiae 8-13 part, Fructus Gardeniae 3-5 part, Herba Asari 11-15 part, Herb Gynostemmae Pentaphylli 4-8 part.

In a preferred embodiment, make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 12 parts, Radix Scutellariae 16 parts, Rhizoma Imperatae 3 parts, 12 parts of Radix Glycyrrhizae, Radix Notoginseng 12 parts, Herba Agrimoniae 10 parts, 9 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 15 parts, Rhizoma Chuanxiong 15 parts, Radix Gentianae Macrophyllae 8 parts, Fructus Aurantii 5 parts, Rhizoma Acori Graminei 3 parts, Fructus Schisandrae Sphenantherae 14 parts, Herba Houttuyniae 9 parts, Herba Scutellariae Barbatae 6 parts, Rhizoma Cyperi 8 parts, Herba Rabdosiae glaucocalycis 5 parts, Cortex Mori 1 part, Herba Hedyotidis Diffusae 10 parts, Rhizoma Coptidis 5 parts, Rhizoma Polygoni Cuspidati 16 parts, Lignum Sappan 10 parts, Radix Osbeckiae Crinitae 8 parts, Rhizoma Sparganii 5 parts, Flos Ilicis Asprellae 1 part, Radix Puerariae 10 parts, 6 parts of Poria, the Radix Aucklandiae 8 parts, Fructus Gardeniae 3 parts, Herba Asari 15 parts, Herb Gynostemmae Pentaphylli 4 parts.

In a preferred embodiment, make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 20 parts, Radix Scutellariae 10 parts, Rhizoma Imperatae 8 parts, 5 parts of Radix Glycyrrhizae, Radix Notoginseng 17 parts, Herba Agrimoniae 12 parts, 4 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 20 parts, Rhizoma Chuanxiong 10 parts, Radix Gentianae Macrophyllae 12 parts, Fructus Aurantii 2 parts, Rhizoma Acori Graminei 7 parts, Fructus Schisandrae Sphenantherae 11 parts, Herba Houttuyniae 15 parts, Herba Scutellariae Barbatae 3 parts, Rhizoma Cyperi 13 parts, Herba Rabdosiae glaucocalycis 3 parts, Cortex Mori 3 parts, Herba Hedyotidis Diffusae 5 parts, Rhizoma Coptidis 10 parts, Rhizoma Polygoni Cuspidati 13 parts, Lignum Sappan 3 parts, Radix Osbeckiae Crinitae 13 parts, Rhizoma Sparganii 3 parts, Flos Ilicis Asprellae 3 parts, Radix Puerariae 5 parts, 4 parts of Poria, the Radix Aucklandiae 10 parts, Fructus Gardeniae 4 parts, Herba Asari 13 parts, Herb Gynostemmae Pentaphylli 5 parts.

In a preferred embodiment, make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 13 parts, Radix Scutellariae 11 parts, Rhizoma Imperatae 4 parts, 6 parts of Radix Glycyrrhizae, Radix Notoginseng 13 parts, Herba Agrimoniae 7 parts, 5 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 18 parts, Rhizoma Chuanxiong 11 parts, Radix Gentianae Macrophyllae 9 parts, Fructus Aurantii 4 parts, Rhizoma Acori Graminei 6 parts, Fructus Schisandrae Sphenantherae 12 parts, Herba Houttuyniae 13 parts, Herba Scutellariae Barbatae 4 parts, Rhizoma Cyperi 9 parts, Herba Rabdosiae glaucocalycis 4 parts, Cortex Mori 2 parts, Herba Hedyotidis Diffusae 6 parts, Rhizoma Coptidis 6 parts, Rhizoma Polygoni Cuspidati 14 parts, Lignum Sappan 5 parts, Radix Osbeckiae Crinitae 10 parts, Rhizoma Sparganii 4 parts, Flos Ilicis Asprellae 2 parts, Radix Puerariae 6 parts, 5 parts of Poria, the Radix Aucklandiae 12 parts, Fructus Gardeniae 3 parts, Herba Asari 12 parts, Herb Gynostemmae Pentaphylli 6 parts.

In a preferred embodiment, make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 17 parts, Radix Scutellariae 15 parts, Rhizoma Imperatae 7 parts, 11 parts of Radix Glycyrrhizae, Radix Notoginseng 16 parts, Herba Agrimoniae 8 parts, 8 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 16 parts, Rhizoma Chuanxiong 14 parts, Radix Gentianae Macrophyllae 11 parts, Fructus Aurantii 3 parts, Rhizoma Acori Graminei 5 parts, Fructus Schisandrae Sphenantherae 13 parts, Herba Houttuyniae 14 parts, Herba Scutellariae Barbatae 5 parts, Rhizoma Cyperi 12 parts, Herba Rabdosiae glaucocalycis 3 parts, Cortex Mori 1 part, Herba Hedyotidis Diffusae 10 parts, Rhizoma Coptidis 9 parts, Rhizoma Polygoni Cuspidati 13 parts, Lignum Sappan 7 parts, Radix Osbeckiae Crinitae 11 parts, Rhizoma Sparganii 3 parts, Flos Ilicis Asprellae 1 part, Radix Puerariae 10 parts, 4 parts of Poria, the Radix Aucklandiae 11 parts, Fructus Gardeniae 4 parts, Herba Asari 14 parts, Herb Gynostemmae Pentaphylli 7 parts.

In a preferred embodiment, make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 15 parts, Radix Scutellariae 12 parts, Rhizoma Imperatae 5 parts, 9 parts of Radix Glycyrrhizae, Radix Notoginseng 15 parts, Herba Agrimoniae 9 parts, 7 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 19 parts, Rhizoma Chuanxiong 13 parts, Radix Gentianae Macrophyllae 10 parts, Fructus Aurantii 4 parts, Rhizoma Acori Graminei 4 parts, Fructus Schisandrae Sphenantherae 11 parts, Herba Houttuyniae 11 parts, Herba Scutellariae Barbatae 5 parts, Rhizoma Cyperi 10 parts, Herba Rabdosiae glaucocalycis 5 parts, Cortex Mori 3 parts, Herba Hedyotidis Diffusae 8 parts, Rhizoma Coptidis 8 parts, Rhizoma Polygoni Cuspidati 14 parts, Lignum Sappan 9 parts, Radix Osbeckiae Crinitae 12 parts, Rhizoma Sparganii 5 parts, Flos Ilicis Asprellae 3 parts, Radix Puerariae 8 parts, 6 parts of Poria, the Radix Aucklandiae 9 parts, Fructus Gardeniae 5 parts, Herba Asari 12 parts, Herb Gynostemmae Pentaphylli 7 parts.

In a preferred embodiment, Rhizoma Curcumae 19 parts, Radix Scutellariae 14 parts, Rhizoma Imperatae 6 parts, 7 parts of Radix Glycyrrhizae, Radix Notoginseng 14 parts, Herba Agrimoniae 9 parts, 6 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 17 parts, Rhizoma Chuanxiong 13 parts, Radix Gentianae Macrophyllae 10 parts, Fructus Aurantii 4 parts, Rhizoma Acori Graminei 4 parts, Fructus Schisandrae Sphenantherae 11 parts, Herba Houttuyniae 11 parts, Herba Scutellariae Barbatae 5 parts, Rhizoma Cyperi 10 parts, Herba Rabdosiae glaucocalycis 5 parts, Cortex Mori 3 parts, Herba Hedyotidis Diffusae 8 parts, Rhizoma Coptidis 8 parts, Rhizoma Polygoni Cuspidati 14 parts, Lignum Sappan 4 parts, Radix Osbeckiae Crinitae 9 parts, Rhizoma Sparganii 5 parts, Flos Ilicis Asprellae 3 parts, Radix Puerariae 8 parts, 3 parts of Poria, the Radix Aucklandiae 8 parts, Fructus Gardeniae 5 parts, Herba Asari 15 parts, Herb Gynostemmae Pentaphylli 8 parts.

In a preferred embodiment, described pharmaceutical composition is prepared in following manner:

(1) each medical material of components by weight percent in arbitrary described pharmaceutical composition is taken, add in the ethanol of 8-12 times amount and soak 1.5-2.5 hour, heating extraction 2 times, each 1.5-2 hour, removes supernatant, united extraction liquid, 120 orders filter, the ultrafiltration post ultrafiltration that molecular weight is 8000-12000 then through damming, the extractum of 1.3-1.4 when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C, heating is concentrated into paste, stand for standby use;

(2) being put in 10-12 times amount water by the medicinal residues that ethanol extraction is crossed, be then placed in extraction pot heated and boiled 1.5-2 hour, filter, filtrate is standby; Filtering residue adds water, second time heating, boils 45 minutes, filters, and filtrate is standby;

(3) 2 filtrates in step (2) being combined, by filtered through gauze, add 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued lixiviate 2-3h, every 10min and stir once, and filtered through gauze merges lixiviating solution, is condensed into pasty state;

(4) the pastel decompression recycling ethanol that step (1) paste that obtains of concentration and step (3) obtained to be concentrated into liquor strength be 0.65-0.7g crude drug/mL, after sucking filtration, the relative density of filtrate is about 1.1-1.2 when 20 DEG C; It is centrifuged under the rotating speed of 3000r/min; At 60 DEG C of-80 DEG C of spray drying, obtain dry extract;

(5) dry extract that step (4) prepares is pulverized, add suitable amount of sucrose, dextrin mixing, with ethanol for wetting agent, granulate, dry, granulate, granule can be prepared; Or the dry extract that step (4) prepares be dry, pulverize, make powder; Or dry extract step (4) prepared is pulverized, add appropriate micropowder silica gel, α-galactose mixing, with ethanol for wetting agent, granulate, dry, granulate, encapsulated, prepare capsule; Or dry extract step (4) prepared is pulverized, add suitable amount of sucrose, dextrin mixing, with ethanol for wetting agent, granulate, dry, granulate, tabletting, prepare tablet; Or to above-mentioned steps (4) obtain centrifugal after concentrated solution in add suitable amount of sucrose, essence mixing, sterilizing, fill, prepare medicinal liquid agent.

The invention still further relates to the application in suppressing proliferation of human gastric cancer cell medicine of a kind of described pharmaceutical composition.

The pharmaceutical composition of suppression proliferation of human gastric cancer cell provided by the present invention, it gives full play to the property of medicine of each component with common Chinese herbal medicine for raw material:

Radix Glycyrrhizae, nature and flavor: sweet, flat, there is effect of invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription, be used for treating lack of appetite, weakness of the spleen and stomach, stomachache loose stool, fatigue and weakness, shortness of breath and palpitation, cough with copious phlegm, gastral cavity abdomen, extremity contraction urgency pain, carbuncle sore tumefacting virus, cushion toxicity and strong.

Herba Agrimoniae: latin name AgrimoniaeHerba, bitter in the mouth, puckery, property is put down, GUIXIN, Liver Channel, there is astringing to arrest bleeding, effect of dysentery relieving parasite killing, cure mainly various hemorrhage card, such as: haematemesis, hematuria, have blood in stool, metrorrhagia, spitting of blood, epistaxis, dysentery, impairment caused by overstrain takes off power, carbuncle, traumatic injury, wound hemorrhage etc. is demonstrate,proved.

Radix Notoginseng: the sweet mildly bitter flavor of Radix Notoginseng property, warm in nature, return liver, stomach through being used as medicine with root, root stock. Rare Chinese medicine, raw with can hemostasia and dissipation blood stasis, reducing swelling and alleviating pain, there is good hemostasia effect, significant hemopoietic function; Tightening and improving Coronary microcirculation, Radix Notoginseng is used as medicine with a long history, acts on the peculiar treasured being considered as in medicine by ancient Chinese medicine doctor, therefore has the saying of " Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) ". " taste is micro-sweet and bitter, quite like the taste of Radix Ginseng for Radix Notoginseng. " " all canes flutter trauma, the dripping person of blood stasis, chew namely stopping of rotten net for catching birds or fish immediately, and namely bruise person dissipate. If during by cane, first taking one, two money, then the blood not centre of punch, especially should taking it after cane, puerperal, clothes were also good. On the whole this medicine temperature, sweet and slightly bitter taste, and sun is bright, the medicine of cloudy blood system of fainting, therefore all disorders of blood can be controlled ". " book on Chinese herbal medicine is looked for the truth " is recorded: " Radix Notoginseng, common people only know function hemostasis and pain-relieving. Hardly realizing that pain aches work because of blood stasis, because of deposited dissipating, blood stops blood. Radix Notoginseng abnormal smells from the patient hardship temperature, can in its blood stasis of blood systemization. " Radix Notoginseng can increase coronary flow, decreased heart rate, reduce myocardial oxygen consumption, increase cardiac muscle oxygen-resistant ability, improve microcirculation, release the effect of smooth muscle spasm.

Lignum Sappan, sweet, salty, flat. GUIXIN, liver, spleen channel. Function cures mainly: promoting the circulation of blood blood stasis dispelling, reducing swelling and alleviating pain. For amenorrhea dysmenorrhea, postpartum stagnation, chest and abdomen twinge, wound swells and ache.

Rhizoma Acori Graminei: pungent, bitter, temperature. GUIXIN, stomach warp. Effect with cure mainly: removing dampness whet the appetite, eliminating phlegm of having one's ideas straightened out, refreshment Fructus Alpiniae Oxyphyllae. Not hungry for gastral cavity painful abdominal mass, a mouthful dysentery of keeping silent, the insane disease of coma, forgetful deafness.

Herba Houttuyniae: latin name houttuyniaeherba, acrid in the mouth, cold nature, enters lung, bladder warp, has heat-clearing and toxic substances removing, removing pus and relieving carbuncle, effect of inducing diuresis for treating stranguria syndrome, and for pneumonopathy vomiting pus, expectorant heat syndrome cough is breathed heavily, toxic-heat and blood stasis, damp-heat gonorrhoea, pyretic toxicity carbuncle sore, and snakeworm wants first-class card.

Cortex Mori is the root bark of moraceae plants Mulberry MorusalbaL.. Main product in Anhui, Henan, Zhejiang, Jiangsu, the ground such as Hunan. Effect: eliminating pathogen from the lung for relieving asthma, inducing diuresis to remove edema. For diseases such as lung-heat cough with asthma, the edema of the face, dysuria. " property of medicine opinion ": make, flat. Controlling lung qi dyspnea with fullness of the chest, aqueous vapor edema, main wound is exhausted, dredging water passages, and disappear aqueous vapor, and asthenia exopathogenic heat is had a headache, interior tonifying for the deficiency. " Japan hanako materia medica ": temperature, in tune, the therapeutic method to keep the adverse QI flowing downwards. Expectorant is quenched the thirst, profit intestine and small intestine, appetizing, and lower food kills abdomen and hides worm, and only cholera is vomited and diarrhoea.

Rhizoma Coptidis: bitter in the mouth, cold in nature, nontoxic, GUIXIN, spleen, stomach, liver, gallbladder, large intestine channel. Effect: heat clearing and damp drying, eliminating fire and detoxication. Cure mainly damp and hot feeling of fullness, vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood tell nosebleed, conjunctival congestion, have a toothache, quench one's thirst, carbuncle furuncle, and external treatment eczema, eczema, auditory meatus are suppurated.

Rhizoma Curcumae: pungent, bitter, temperature. Return liver, spleen channel. Removing blood stasis promoting the circulation of QI to relieve pain: this product effect is similar to Rhizoma Sparganii, but temperature concerted effort is relatively big, can treat amenorrhea due to stagnation of blood stomachache, abdominal mass, gather. Long-pending eliminating stagnation: this product energy circulation of qi promoting removing food stagnancy pain relieving, vexed has a pain for eating accumulation, chest and abdomen are full. The lump in the abdomen addiction block that removing blood stasis, women's amenorrhea, or phlegm-damp congestion condense. Promoting the circulation of QI to relieve pain: be suitable to drinking and eating irregularly, the card of gastral cavity abdomen overflow pain. Suitable compatibility, deficient syndrome, the case of a physically strong patient running a high fever.

Rhizoma Cyperi, hardship pungent, micro-, micro-sweet, flat. Return liver, spleen, tri-jiao channel. Function cures mainly: promoting QI circulation for relieving depression, menstruction regulating and pain relieving. For stagnation of QI due to depression of the liver, breast, the side of body, abdominal distention, dyspepsia, breast gastral cavity painful abdominal mass is vexed, and colic of cold type is suffered from abdominal pain, distending pain of the breast, menoxenia, amenorrhea dysmenorrhea.

Radix Gentianae Macrophyllae, toil, flat. Enter liver, stomach, gallbladder meridian. Function cures mainly: expelling wind and removing dampness, and blood relaxing muscles and tendons, clearing away heat and promoting diuresis. Dispelling the wind and dampness pathogens arthralgia pain, muscles and bones contracture, jaundice, have blood in stool, osteopyrexia and fever, children's's infantile malnutrition with fever, dysuria.

Radix Ophiopogonis, sweet, micro-hardship, it is slightly cold. GUIXIN, lung, stomach warp. YIN nourishing and the production of body fluid promoting, lung moistening clears away heart-fire. For dryness of the lung dry cough. Chronic consumptive disease is coughed, and Tianjin wound is thirsty, vexed insomnia, and interior-heat is quenched one's thirst, dryness of the intestine constipation; Pharyngeal diphtheria.

Fructus Aurantii nature and flavor are bitter, acid, be slightly cold, and return lung, spleen, liver, stomach, large intestine channel, and function cures mainly after septum pectorale feeling of fullness, distending pain over the hypochondrium, accumulation of food in the stomach and intes tine due to indigestion, distension and fullness in the abdomen, dysentery the diseases such as weight, proctoptosis, uterine prolapse.

The Radix Aucklandiae, pungent, bitter, temperature. Return spleen, stomach, large intestine, three burnt, gallbladder meridians. Function cures mainly: promoting the circulation of QI to relieve pain, strengthening the spleen to promote digestion. For breast gastral cavity distending pain, weight after dysentery, food stagnation does not disappear, anorexia. Radix Aucklandiae (roasted) reality intestinal antidiarrheal. For stomachache of having loose bowels.

Fructus Schisandrae Sphenantherae, sour, sweet, temperature. Return lung, the heart, kidney channel. Function cures mainly: restrain astringent or styptic treatment for spontaneous sweating, supplementing QI for promoting the production of body fluid, kidney calming. For chronic cough dyspnea due to deficiency, emission, enuresis frequent micturition, incessant chronic diarrhea, spontaneous perspiration, night sweat, Tianjin wound is thirsty, and deficient pulse of losing heart, interior-heat is quenched one's thirst, palpitation and insomnia.

Fructus Amomi Rotundus, pungent, temperature. Return lung, spleen, stomach warp. Removing dampness disappears painful abdominal mass, circulation of qi promoting warming middle-JIAO, appetite-stimulating indigestion-relieving. For turbid damp obstructing in middle-JIAO, anorexia, hygropyrexia from the beginning of, uncomfortable in chest not hungry, cold-damp vomiting, chest and abdomen swelling and pain, food stagnation does not disappear.

Flos Ilicis Asprellae, bitter, sweet, cool. Function cures mainly: heat-clearing and toxic substances removing, promoting the production of body fluid to quench thirst. For catching a cold, high hot excessive thirst, tonsillitis, pharyngolaryngitis, tracheitis, pertussis, enteritis, dysentery, infectious hepatitis, wild gill fungus, arsenic poisoning. For herbal tea primary raw material; Traumatic injury is controlled in leaf external, carbuncle furuncle and phyma poison.

Herba Rabdosiae glaucocalycis: acrid in the mouth, hardship, cool in nature. Effect: heat-clearing and toxic substances removing, dissipating blood stasis for subsidence of swelling. Cure mainly venom, treating swelling and pain by traumatic injury, muscles and bones are ached, skin infection.

Rhizoma Imperatae, sweet, cold. Return lung, stomach, bladder warp. Function cures mainly: cooling blood for hemostasis, clearing away heat and promoting diuresis. Spit blood for heat in blood, epistaxis, hematuria, calentura excessive thirst, jaundice, edema, the puckery pain of pyretic stranguria; Acute nephritis edema.

Herba Scutellariae Barbatae: cool in nature, mildly bitter flavor. Return lung, liver, kidney channel. Tool heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, the function such as anticancer. For furuncle swelling toxin, laryngopharynx swelling and pain, venom, fall and flutter the pain of injury, edema, jaundice.

Herba Hedyotidis Diffusae: bitter in the mouth, sweet, cold in nature, enter the heart, liver, spleen, large intestine channel, there is heat-clearing and toxic substances removing, the effect of promoting diuresis to eliminate damp pathogen, for dyspnea and cough due to lung-heat, laryngopharynx swelling and pain, acute appendicitis, furuncle and phyma skin infection, venom, the puckery pain of pyretic stranguria, edema, dysentery, enteritis, jaundice due to damp-heat, cancerous protuberances etc. are demonstrate,proved, and often utilize Herba Hedyotidis Diffusae anticancer, and it has certain radiation resistance.

Rhizoma Polygoni Cuspidati: this product is dry rhizome and the root of polygonaceae plant Rhizoma Polygoni Cuspidati PolygoumcuspidatumSieb.etZucc.. This product mostly is cylindrical short section or irregular sheet, long 1-7cm, diameter 0.5-2.5cm. Crust sepia, has vertical wrinkle and mark of fibrous root, and tangent plane skin zone is relatively thin, and woody part is broad, brown color, ray irradiation shape, and skin zone is more easily separated with woody part. Rhizome marrow has every or in cavity shape. Matter is hard. Feeble QI is mildly bitter flavor, puckery. Meridian distribution of property and flavor: micro-hardship, is slightly cold. Return liver, gallbladder, lung meridian. Function cures mainly: dampness removing jaundice eliminating, heat-clearing and toxic substances removing, eliminating stasis to stop pain, relieving cough and resolving phlegm. For jaundice due to damp-heat, stranguria with turbid discharge, leukorrhagia, rheumatic arthralgia, carbuncle sore tumefacting virus, burn due to hot liquid or fire, amenorrhea, abdominal mass, injury from falling down, cough due to lung-heat.

Radix Osbeckiae Crinitae: sweet in the mouth, puckery, property is put down. Effect: clearing away heat-damp and promoting diuresis, cough-relieving, regulating menstruation. Cure mainly acute gastroenteritis, bacillary dysentery, dyspepsia, chronic bronchitis, spit blood, menoxenia, leucorrhea.

Poria: sweet in the mouth, light, property are put down, can eliminating dampness and diuresis; Invigorating the spleen and regulating the stomach; Mind tranquilizing and the heart calming. Cure mainly dysuria; Edema distension; Phlegm retention cough with dyspnea; Vomiting; Insufficiency of the spleen lack of appetite; Have loose bowels; Palpitation and restlessness; Insomnia forgetfulness; Seminal emission nebulousurine.

Radix Scutellariae: base: this product is the dry root of labiate Radix Scutellariae. Character: this product is conical. Surface brown color or buff, have sparse excipuliform radicula trace. Top is rougher, has the vertical wrinkle of distortion or irregular reticulate pattern, and rift grain and scabriculous are arranged at bottom. Nature and flavor: bitter, cold. Gui Jing: return lung, gallbladder, spleen, large intestine, small intestine meridian. Effect: heat clearing and damp drying, rushes down people's removing toxic substances, stops blooding, antiabortive. Cure mainly: for hygropyrexia, fever disease in summer vomiting and nausea uncomfortable in chest, damp and hot feeling of fullness, dysentery, jaundice, cough due to lung-heat, high hot excessive thirst, heat in blood tells nosebleed, carbuncle sore tumefacting virus, frequent fetal movement.

Rhizoma Sparganii: acrid in the mouth; Puckery; Cool. Enter liver. Spleen channel. Controlling lump in the abdomen, gather, distending pain over the hypochondrium, food stagnation distending pain, dysmenorrhea, amenorrhea, postnatal blood stasis is suffered from abdominal pain, traumatic injury. Cough due to lung-heat controlled by mongolian medicine, abundant expectoration of panting, liver-heat, arteries and veins heat, the hot hectic fever due to YIN-deficiency of consumptive disease, and " precious day " is sick, fracture.

Its cold in nature, bitter in the mouth of Fructus Gardeniae, function with cure mainly: pathogenic fire purging relieving restlessness, clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body, Shennong's Herbal: " cure mainly pathogen in five, tuberculoid leprosy, red leprosy, skin infection .... "

Herb Gynostemmae Pentaphylli: sweet in the mouth, hardship, cold nature; Return lung meridian, spleen channel, kidney channel. Energy QI invigorating, calms the nerves, blood pressure lowering, heat-clearing and toxic substances removing, eliminating phlegm and stopping cough. After small number of patients is taken medicine, the symptoms such as nausea and vomiting, abdominal distention diarrhoea (or constipation), dizziness, dim eyesight, tinnitus occur. After taking Herb Gynostemmae Pentaphylli, enterogastric peristalsis is accelerated, and it is long that appetite increases the resistance to time hungered and thirst, and not only treats constipation but also control loose stool. Reaction that is cerebral cortex is excited and that suppress can keep suitable balance, and nervus centralis is had dual regulation. There is excellent analgesia, effect sleeping, antitonic. In reduction myocardial wall on the basis of nervous, mitigation cerebrovascular and peripheral vascular resistance, heart and brain vigor can be strengthened, strengthen coronary flow, relax arteriosclerosis, promote entirety circulation more vigorous and smooth. It is demonstrated experimentally that diet is rich and take Herb Gynostemmae Pentaphylli group, refusing to obey Herb Gynostemmae Pentaphylli group with avoiding meat, make regular check on and compare, internal gallbladder is solid and neutral fat content is close. Body fat mass in human body is had benign transformed adjustment effect by Herb Gynostemmae Pentaphylli as can be seen here. Taking Herb Gynostemmae Pentaphylli to compare with the mouse of disobedience, the about long 31-57% of its swimming time, and antisecosis is also faster, after people takes Herb Gynostemmae Pentaphylli, muscle power energy is more abundant, indefatigability, is also easy to recover even if tired, thus work efficiency increases. Human body part function is weak, and Cucumber can produce allergy, and formed bronchus pant, the disease such as skin rashes, arthralgia, Herb Gynostemmae Pentaphylli is rather good to these sick effects.

Radix Puerariae, sweet in the mouth, pungent, cool in nature. Enter spleen, stomach warp. There is yang invigorating expelling pathogenic factors from muscles, rash antidiarrheal, effect of relieving restlessness only temperature. For typhoid fever, warm headache stiffness of the nape, dysphoria with smothery sensation is quenched one's thirst, and has loose bowels, dysentery, and skin eruption is not saturating, hypertension, and angina pectoris is deaf. It is used for Hypertension neck pain, angina pectoris clinically, but also there is hypoglycemic activity.

Rhizoma Chuanxiong, nature and flavor are pungent, temperature. Enter liver, gallbladder meridian. Curing mainly activating QI to alleviate the depression, method wind-dryness is wet, promoting blood circulation and stopping pain. Controlling air-cooled headache rotation dizzy, hypochondriac pain abdomen aches, arthralgia due to cold clonic spasm of the muscle, amenorrhea, difficult labour, postpartum stagnation block pain, ulcer sores. For menoxenia, amenorrhea dysmenorrhea, abdominal mass is suffered from abdominal pain, breast side of body twinge, tumbling and swelling, headache, rheumatic arthralgia.

Herba Asari: nature and flavor are pungent, warm; Effect: dispel the wind, cold expelling, row water, have one's ideas straightened out. Herba Asari can outer dispersing wind and cold, again can in dispel YIN-cold, pain relieving simultaneously.

The present invention is by after above drug regimen, and drug effect has synergism, has blood circulation promoting and blood stasis dispelling, heat-clearing and toxic substances removing, hard masses softening and resolving, improves the effect of immunity, can significantly improve gastric environment, the increment of effective anticancer and diffusion. It is applied in the treatment of gastric cancer, can not only comprehensively suppress the development of cancerous protuberance, and can well improve patient immune function, improve patient self anti-tumor capacity, suppress late tumor transfer in time, and effectively reduce the generation of late complication. The pharmaceutical composition compatibility suppressing proliferation of human gastric cancer cell of the present invention meets Chinese medicine " monarch " principle, give prominence to the key points, comprehensive and reasonable, strong drug action, the efficacy of a drug are rapid, efficacy stability, there is heat-clearing and toxic substances removing, effect of blood circulation promoting and blood stasis dispelling, to suppressing proliferation of human gastric cancer cell to have good result, and has features such as not easily recurring, have no side effect.

Detailed description of the invention

For making the purpose of the embodiment of the present invention, technical scheme and advantage clearly, below in conjunction with embodiment, technical scheme is clearly and completely described. Obviously, described embodiment is the part of the present invention, rather than whole embodiments. Based on described embodiments of the invention, the every other embodiment that those of ordinary skill in the art obtain under the premise without creative work, broadly fall into the scope of protection of the invention. Except as otherwise noted, the percent of the amount of alcohol in the present invention is percentage by volume, and v/v represents the volume ratio of solution.

Embodiment 1:

A kind of pharmaceutical composition suppressing proliferation of human gastric cancer cell of the present invention, take following traditional Chinese medicine raw medicine: Rhizoma Curcumae 12 grams, Radix Scutellariae 16 grams, Rhizoma Imperatae 3 grams, 12 grams of Radix Glycyrrhizae, Radix Notoginseng 12 grams, Herba Agrimoniae 10 grams, 9 grams of Fructus Amomi Rotundus, Radix Ophiopogonis 15 grams, Rhizoma Chuanxiong 15 grams, Radix Gentianae Macrophyllae 8 grams, Fructus Aurantii 5 grams, Rhizoma Acori Graminei 3 grams, Fructus Schisandrae Sphenantherae 14 grams, Herba Houttuyniae 9 grams, Herba Scutellariae Barbatae 6 grams, Rhizoma Cyperi 8 grams, Herba Rabdosiae glaucocalycis 5 grams, Cortex Mori 1 gram, Herba Hedyotidis Diffusae 10 grams, Rhizoma Coptidis 5 grams, Rhizoma Polygoni Cuspidati 16 grams, Lignum Sappan 10 grams, Radix Osbeckiae Crinitae 8 grams, Rhizoma Sparganii 5 grams, Flos Ilicis Asprellae 1 gram, Radix Puerariae 10 grams, 6 grams of Poria, the Radix Aucklandiae 8 grams, Fructus Gardeniae 3 grams, Herba Asari 15 grams, Herb Gynostemmae Pentaphylli 4 grams.

Embodiment 2:

A kind of pharmaceutical composition suppressing proliferation of human gastric cancer cell of the present invention, take following traditional Chinese medicine raw medicine: Rhizoma Curcumae 20 grams, Radix Scutellariae 10 grams, Rhizoma Imperatae 8 grams, 5 grams of Radix Glycyrrhizae, Radix Notoginseng 17 grams, Herba Agrimoniae 12 grams, 4 grams of Fructus Amomi Rotundus, Radix Ophiopogonis 20 grams, Rhizoma Chuanxiong 10 grams, Radix Gentianae Macrophyllae 12 grams, Fructus Aurantii 2 grams, Rhizoma Acori Graminei 7 grams, Fructus Schisandrae Sphenantherae 11 grams, Herba Houttuyniae 15 grams, Herba Scutellariae Barbatae 3 grams, Rhizoma Cyperi 13 grams, Herba Rabdosiae glaucocalycis 3 grams, Cortex Mori 3 grams, Herba Hedyotidis Diffusae 5 grams, Rhizoma Coptidis 10 grams, Rhizoma Polygoni Cuspidati 13 grams, Lignum Sappan 3 grams, Radix Osbeckiae Crinitae 13 grams, Rhizoma Sparganii 3 grams, Flos Ilicis Asprellae 3 grams, Radix Puerariae 5 grams, 4 grams of Poria, the Radix Aucklandiae 10 grams, Fructus Gardeniae 4 grams, Herba Asari 13 grams, Herb Gynostemmae Pentaphylli 5 grams.

Embodiment 3:

A kind of pharmaceutical composition suppressing proliferation of human gastric cancer cell of the present invention, take following traditional Chinese medicine raw medicine: Rhizoma Curcumae 13 grams, Radix Scutellariae 11 grams, Rhizoma Imperatae 4 grams, 6 grams of Radix Glycyrrhizae, Radix Notoginseng 13 grams, Herba Agrimoniae 7 grams, 5 grams of Fructus Amomi Rotundus, Radix Ophiopogonis 18 grams, Rhizoma Chuanxiong 11 grams, Radix Gentianae Macrophyllae 9 grams, Fructus Aurantii 4 grams, Rhizoma Acori Graminei 6 grams, Fructus Schisandrae Sphenantherae 12 grams, Herba Houttuyniae 13 grams, Herba Scutellariae Barbatae 4 grams, Rhizoma Cyperi 9 grams, Herba Rabdosiae glaucocalycis 4 grams, Cortex Mori 2 grams, Herba Hedyotidis Diffusae 6 grams, Rhizoma Coptidis 6 grams, Rhizoma Polygoni Cuspidati 14 grams, Lignum Sappan 5 grams, Radix Osbeckiae Crinitae 10 grams, Rhizoma Sparganii 4 grams, Flos Ilicis Asprellae 2 grams, Radix Puerariae 6 grams, 5 grams of Poria, the Radix Aucklandiae 12 grams, Fructus Gardeniae 3 grams, Herba Asari 12 grams, Herb Gynostemmae Pentaphylli 6 grams.

Embodiment 4:

A kind of pharmaceutical composition suppressing proliferation of human gastric cancer cell of the present invention, take following traditional Chinese medicine raw medicine: Rhizoma Curcumae 17 grams, Radix Scutellariae 15 grams, Rhizoma Imperatae 7 grams, 11 grams of Radix Glycyrrhizae, Radix Notoginseng 16 grams, Herba Agrimoniae 8 grams, 8 grams of Fructus Amomi Rotundus, Radix Ophiopogonis 16 grams, Rhizoma Chuanxiong 14 grams, Radix Gentianae Macrophyllae 11 grams, Fructus Aurantii 3 grams, Rhizoma Acori Graminei 5 grams, Fructus Schisandrae Sphenantherae 13 grams, Herba Houttuyniae 14 grams, Herba Scutellariae Barbatae 5 grams, Rhizoma Cyperi 12 grams, Herba Rabdosiae glaucocalycis 3 grams, Cortex Mori 1 gram, Herba Hedyotidis Diffusae 10 grams, Rhizoma Coptidis 9 grams, Rhizoma Polygoni Cuspidati 13 grams, Lignum Sappan 7 grams, Radix Osbeckiae Crinitae 11 grams, Rhizoma Sparganii 3 grams, Flos Ilicis Asprellae 1 gram, Radix Puerariae 10 grams, 4 grams of Poria, the Radix Aucklandiae 11 grams, Fructus Gardeniae 4 grams, Herba Asari 14 grams, Herb Gynostemmae Pentaphylli 7 grams.

Embodiment 5:

A kind of pharmaceutical composition suppressing proliferation of human gastric cancer cell of the present invention, take following traditional Chinese medicine raw medicine: Rhizoma Curcumae 15 grams, Radix Scutellariae 12 grams, Rhizoma Imperatae 5 grams, 9 grams of Radix Glycyrrhizae, Radix Notoginseng 15 grams, Herba Agrimoniae 9 grams, 7 grams of Fructus Amomi Rotundus, Radix Ophiopogonis 19 grams, Rhizoma Chuanxiong 13 grams, Radix Gentianae Macrophyllae 10 grams, Fructus Aurantii 4 grams, Rhizoma Acori Graminei 4 grams, Fructus Schisandrae Sphenantherae 11 grams, Herba Houttuyniae 11 grams, Herba Scutellariae Barbatae 5 grams, Rhizoma Cyperi 10 grams, Herba Rabdosiae glaucocalycis 5 grams, Cortex Mori 3 grams, Herba Hedyotidis Diffusae 8 grams, Rhizoma Coptidis 8 grams, Rhizoma Polygoni Cuspidati 14 grams, Lignum Sappan 9 grams, Radix Osbeckiae Crinitae 12 grams, Rhizoma Sparganii 5 grams, Flos Ilicis Asprellae 3 grams, Radix Puerariae 8 grams, 6 grams of Poria, the Radix Aucklandiae 9 grams, Fructus Gardeniae 5 grams, Herba Asari 12 grams, Herb Gynostemmae Pentaphylli 7 grams.

Embodiment 6:

A kind of pharmaceutical composition suppressing proliferation of human gastric cancer cell of the present invention, take following traditional Chinese medicine raw medicine: Rhizoma Curcumae 19 grams, Radix Scutellariae 14 grams, Rhizoma Imperatae 6 grams, 7 grams of Radix Glycyrrhizae, Radix Notoginseng 14 grams, Herba Agrimoniae 9 grams, 6 grams of Fructus Amomi Rotundus, Radix Ophiopogonis 17 grams, Rhizoma Chuanxiong 13 grams, Radix Gentianae Macrophyllae 10 grams, Fructus Aurantii 4 grams, Rhizoma Acori Graminei 4 grams, Fructus Schisandrae Sphenantherae 11 grams, Herba Houttuyniae 11 grams, Herba Scutellariae Barbatae 5 grams, Rhizoma Cyperi 10 grams, Herba Rabdosiae glaucocalycis 5 grams, Cortex Mori 3 grams, Herba Hedyotidis Diffusae 8 grams, Rhizoma Coptidis 8 grams, Rhizoma Polygoni Cuspidati 14 grams, Lignum Sappan 4 grams, Radix Osbeckiae Crinitae 9 grams, Rhizoma Sparganii 5 grams, Flos Ilicis Asprellae 3 grams, Radix Puerariae 8 grams, 3 grams of Poria, the Radix Aucklandiae 8 grams, Fructus Gardeniae 5 grams, Herba Asari 15 grams, Herb Gynostemmae Pentaphylli 8 grams.

Wherein, the preparation method of the described Chinese medicine composition of embodiment 1-6 is:

(1) each medical material of components by weight percent in the arbitrary described Chinese medicine composition of Example 1-6, add in the ethanol of 8-12 times amount and soak 1.5-2.5 hour, heating extraction 2 times, each 1.5-2 hour, removes supernatant, united extraction liquid, 120 orders filter, the ultrafiltration post ultrafiltration that molecular weight is 8000-12000 then through damming, the extractum of 1.3-1.4 when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C, heating is concentrated into paste, stand for standby use;

Acute toxicity test:

Take mice 60, body weight 22-25 gram, to mouse stomach capsule solution, the median lethal dose(LD 50) (LD50) of administration has been carried out test determination, but has not measured LD50, therefore carried out mtd test. Result: with maximum dosage-feeding 120g/kg gavage, is equivalent to clinical intend daily dosage 280 times of being grown up, none death of mice in seven days, puts to death each internal organs of mice anatomic observation and does not find toxic reaction, and administration group and control group mice are without any difference.

Six months long term toxicity tests: take rat 60, body weight 180～220g, Experimental agents is set as high, medium and low three dosage, respectively capsule 40g/kg, 20g/kg, 10g/kg, within 1.1g/kg/ days, calculate with (75kg is standard) dosage by people, for 40 times of people's effective dose, 20 times and 10 times; Separately set a matched group, after continuous gastric infusion 90 days, 180 days and Some Animals drug withdrawal 10 days, observe the impact on the every physiology of rat, biochemical indicator. Test result indicate that: by various dose to rat continuous oral 180 days, food-intake and body weight on rat increase all without affecting. Hepatic and renal function is not all found overt toxicity effect. Routine blood test, blood biochemistry, urine biochemistry and bone marrow sheet etc. are showed no and significantly affect, between each group, compare no significant difference with matched group.

The tablet of the embodiment of the present invention 3 experimentation data to suppressing Mouse Gastric Cancer cell MFC cell proliferation:

Experiment material

Experiment cell strain: Mouse Gastric Cancer cell MFC cell, University Of Qingdao's laboratory cell storehouse, DMEM+10%FBS cellar culture.

Experimental agents: the tablet that the embodiment of the present invention 3 is made, weighs 100mg tablet, is dissolved in 5ml dehydrated alcohol, 0.2 μm of frit, 500 μ ldoff pipe subpackages ,-20 DEG C of storages, and simultaneously 0.2 μm of frit dehydrated alcohol is in order to the use of matched group.

Experiment reagent: DMEM (GIBCO company Cat.No.12100-061Lot.No.758137); Hyclone (Tian Hang bio tech ltd, Zhejiang Lot.No.100419); NaHC03 (Shanghai hundred million chemical reagent company limited Cat.No.11810-033Lot.No.1088387 of a specified duration); Trypsin (AMRESCO company); EDTA (AMRESCO company); PenicillinGSodiumSalt (AMRESCO company 1); StreptomycinSulfate (AMRESCO); Dehydrated alcohol (Zibo Ya Dulan Trade Co., Ltd.); MTT (Biosharp lot number: 0793): PBS (laboratory autogamy);

Experiment equipment: Lycra inverted microscope (Germany Leica model: DMIL); Visible-ultraviolet light microwell plate detector (MD company of U.S. model: SPECTRAMAX190); CO₂Incubator (FORMA model: 3111); Super-clean bench (safe and sound company of Su Jing group manufactures model: SW-CJ-ZFD); Pure water instrument (Sprlng company of U.S. model: S/N020579); Accurate pipettor (Gilson Inc of France model: P2); Electronic balance (Sai Duolisi company limited of Germany model: BT323S); Full-automatic high-pressure autoclave (SANYO company of Japan model: MLS-3020); Table electrothermal air dry oven (Shanghai precision experimental facilities company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μm of filter (MILLIPORE model: SLGP033RB); 1cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.

Experimental technique:

(1) MFC cell DMEM+10%FBS in 37 DEG C, 5%C02 carry out cellar culture (10cm culture dish), when Growth of Cells to logarithmic (log) phase, collect cell, discard culture fluid, PBS fine laundering 3 times, add 3ml0.25% trypsin-0.04%EDTA, after 37 DEG C of digestion 2min, it is added thereto in 5ml complete medium and reaction, is proceeded in centrifuge tube after piping and druming cell, 1000rpm is centrifuged 5min, adjusts concentration of cell suspension 3 × 10⁴Individual/ml.

(2) entering in 96 well culture plates by cell kind, every hole adds cell suspension 180 μ l, culture plate put in cell culture incubator (37 DEG C, 5%CO₂) cellar culture.

(3) according to cell growth status, general long to 50%-70%, add sheet agent solution, continue to cultivate 24h.

(4) add 20 μ lMTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.

(5) after 4h, buckle method removes supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, makes crystal fully dissolve. The light absorption value in each hole is measured at enzyme-linked immunosorbent assay instrument 490nm place.

(6) arranging background (being not added with cell, only add culture fluid), control wells (cell, the medicine dissolution medium of same concentrations, culture fluid, MTT, dimethyl sulfoxide), often group sets 6 multiple holes simultaneously.

(7) suppression ratio of cell is represented by result with medicine: cell proliferation suppression ratio (%)=(control wells OD value-dosing holes OD value), control wells OD value × 100%. Experiment repeats 3 times.

Statistical disposition: adopting the correlation analysis in MicrosoftExcel2007 software and Studentt inspection, data represent with mean ± S.D..

Experimental result:

Statistical result showed after mtt assay experiment, compare with matched group, when dosage reaches 5mg/ml, to MFC cell inhibitory effect variant (P < 0.05), dosage this difference when 10mg/ml has significance (P < 0.01), has pole significant difference (P < 0.001) when dosage reaches 15-20mg/ml.

The tablet that table 1 embodiment of the present invention 3 is made is to MFC cell inhibitory effect influence research (X ± SD)

Group	Drug level (mg/ml)	Suppression ratio (%)
			Matched group	0	0
1	5	9.28±3.28
			2	10	19.11±5.37*
3	15	27.85±8.19**
			4	20	34.28±10.39**

Note: compare with matched group, * P < 0.01; * P < 0.001.

Experiment conclusion: the tablet that the embodiment of the present invention 3 is made can effectively suppress Mouse Gastric Cancer cell MFC cell proliferation, reduces the Growth of Cells number of MFC cell, and this effect is dose dependent.

Clinical reasoning:

In order to verify the effect of pharmaceutical composition of the present invention, in May ,-2014 in October, 2012,300 example patients with gastric cancer clinical observations are randomly drawed in outpatient service place, in the object making a definite diagnosis observation, male 148 example, women 152 example, without other serious disease, the age is 35 years old-79 years old, 59.5 years old mean age.

Diagnostic criteria: according in " China common cancer diagnosis and treatment specification " (the 4th gastric cancer) that China's nineteen ninety publishes the precancerous lesion of gastric cancer being defined as foundation.

Therapeutic Method: take the tablet of the embodiment of the present invention 3, takes at twice sooner or later, and each 2-3 sheet, 10 days is 1 course for the treatment of, altogether 3-6 the course for the treatment of of treatment.

Efficacy assessment standard:

Effective: body constitution strengthens, limbs are strong, and abdominal discomfort disappears, and tumor stomach tumor body reduces, and other position has no nascent tumor.

Effective: body constitution strengthens, and tumor stomach has no increase, and other position has no nascent tumor.

Invalid: symptom is unchanged, tumor stomach tumor body increases, or nascent tumor occurs in other position.

Therapeutic outcome: effective 243 examples, obvious effective rate is: 81.0%; Effective 48 examples, effective percentage is: 16.0%; No effect 9, inefficiency is: 3.0%, total effective rate: 97.0%. Visible, the pharmaceutical composition of the present invention, to suppressing proliferation of human gastric cancer cell to have significant curative effect, is suitable to clinical application.

The model case of inventor daily clinic contact is presented herein below:

Case 1:

Zhao, man, 64 years old, people from South, Qingdao City. Patient goes to a doctor in March, 2013, and patient is old gastropathy throughout the year, and health is always weaker, and nearest stomach is vexed swollen, and stomachache is serious, anorexia of feeling sick. It is diagnosed as Gerontal gastric cancer, the maximum tumor 3.1cm of stomach × 2.8cm through hospital. Taking the oral liquid of the present embodiment 2, one day twice, each one bag, sooner or later one after each meal, 10 days is a course for the treatment of, and after using two courses for the treatment of, stomach discomfort symptom substantially alleviates, and the phenomenon such as various pain symptoms and nauseating anorexia is greatly improved; After five courses for the treatment of, patient feels that health is solid as before disease, and body weight too increases. The maximum tumor of stomach has been contracted to 2.6cm × 2.1cm. Recurrence of following up a case by regular visits to that so far there are no.

Case 2:

Money, female, 56 years old, people from Lixia District, Jinan City. In February, 2014 goes to a doctor in here. Patient feels hypopharynx difficulty of having a meal, and Nausea and vomiting, appetite one declines, hence it is evident that become thin. It is diagnosed as gastric cancer through hospital gastroscope inspection. The tumor of a 2.8cmx1.6cm is had at stomach cardia place. Patient age is relatively big, physical weakness, and operative treatment ratio is relatively hazardous, probably brings negative consequence, adopts and takes Drug therapy of the present invention. Take the granule of the embodiment of the present invention 6, one day twice, each two bags, one after each meal sooner or later, it within 10 days, it is a course for the treatment of, after using two courses for the treatment of, hence it is evident that feels pain alleviates, can eating spoon meat, after continuing to take five course for the treatment of medicines, above-mentioned symptom all substantially alleviates or disappears. Tumor body has been contracted to 2.4cmx1.2cm on inspection, and old man's health is hale and hearty, within 1 year so far, has no recurrence.

Case 3:

Grandson, man, 60 years old, people from Laiyang, Yantai. Patient goes to a doctor in November, 2013, patient's stomach medical history 20 years, starts stomachache severity, stomach distending pain, have a meal and do not have appetite, day by day become thin before two-and-a-half years. It is diagnosed as Gerontal gastric cancer through hospital, then does stomach big portion resection operation at local hospital. Within postoperative 1 year, patient feels flatulence pain, has grown again the tumor of a 0.8cm × 1.2cm on inspection, diagnose as recurrence of gastric cancer by the anastomotic stoma of stomach. Because patient body weakness is not suitable for again performing the operation, start medicine partner treatment of the present invention of taking medicine later. Taking the capsule of the embodiment of the present invention 5, one day twice, each two, one after each meal, 10 days is a course for the treatment of sooner or later, and after using two courses for the treatment of, the state of an illness of patient is greatly improved, and flatulence, stomachache situation disappear. Continuing to take medicine after four courses for the treatment of, diet is ratio compared with normal all, has done comprehensive inspection, tumor disappearance in hospital, patient add again one the course for the treatment of medicine with after treatment effect, follow up a case by regular visits to 1 year, have no recurrence.

Case 4:

Lee, female, 49 years old, people from Linzhou City, Zibo. In April, 2013 goes to a doctor, symptom: hypopharynx difficulty of eating, weight loss, intermittent stomach pain is with serious vomiting, and removing hospital diagnosis is early gastric cancer. Sick age: 5 months. Taking the granule of the embodiment of the present invention 6, one day twice, each two bags, one after each meal, 10 days is a course for the treatment of sooner or later, and after using two courses for the treatment of, symptoms of emesis alleviates, and pain has very big alleviation; Continuing to take 4 courses for the treatment of, function of deglutition recovers completely, substantially diminishes through examination in hospital tumor. Being continuing with traditional Chinese medicine composition for treating of the present invention 3 months, tumor disappearance, so far without recurrence.

Last it is noted that above example is only in order to illustrate technical scheme, it is not intended to limit; Although the present invention being described in detail with reference to previous embodiment, it will be understood by those within the art that: the technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature is carried out equivalent replacement; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the scope of various embodiments of the present invention technical scheme.

Claims

1. the pharmaceutical composition suppressing proliferation of human gastric cancer cell, it is characterised in that: the crude drug of described pharmaceutical composition is made up of Radix Glycyrrhizae, Herba Agrimoniae, Radix Notoginseng, Lignum Sappan, Rhizoma Acori Graminei, Herba Houttuyniae, Cortex Mori, Rhizoma Coptidis, Rhizoma Curcumae, Rhizoma Cyperi, Radix Gentianae Macrophyllae, Radix Ophiopogonis, Fructus Aurantii, the Radix Aucklandiae, Fructus Schisandrae Sphenantherae, Fructus Amomi Rotundus, Flos Ilicis Asprellae, Herba Rabdosiae glaucocalycis, Rhizoma Imperatae, Herba Scutellariae Barbatae, Herba Hedyotidis Diffusae, Rhizoma Polygoni Cuspidati, Radix Osbeckiae Crinitae, Poria, Radix Scutellariae, Rhizoma Sparganii, Fructus Gardeniae, Herb Gynostemmae Pentaphylli, Radix Puerariae, Rhizoma Chuanxiong, Herba Asari.

2. the pharmaceutical composition of suppression proliferation of human gastric cancer cell according to claim 1, it is characterized in that: make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 12-20 part, Radix Scutellariae 10-16 part, Rhizoma Imperatae 3-8 part, Radix Glycyrrhizae 5-12 part, Radix Notoginseng 12-17 part, Herba Agrimoniae 7-12 part, Fructus Amomi Rotundus 4-9 part, Radix Ophiopogonis 15-20 part, Rhizoma Chuanxiong 10-15 part, Radix Gentianae Macrophyllae 8-12 part, Fructus Aurantii 2-5 part, Rhizoma Acori Graminei 3-7 part, Fructus Schisandrae Sphenantherae 11-14 part, Herba Houttuyniae 9-15 part, Herba Scutellariae Barbatae 3-6 part, Rhizoma Cyperi 8-13 part, Herba Rabdosiae glaucocalycis 3-5 part, Cortex Mori 1-3 part, Herba Hedyotidis Diffusae 5-10 part, Rhizoma Coptidis 5-10 part, Rhizoma Polygoni Cuspidati 13-16 part, Lignum Sappan 3-10 part, Radix Osbeckiae Crinitae 8-13 part, Rhizoma Sparganii 3-5 part, Flos Ilicis Asprellae 1-3 part, Radix Puerariae 5-10 part, Poria 3-6 part, Radix Aucklandiae 8-13 part, Fructus Gardeniae 3-5 part, Herba Asari 11-15 part, Herb Gynostemmae Pentaphylli 4-8 part.

3. the pharmaceutical composition of suppression proliferation of human gastric cancer cell according to claim 1, it is characterized in that: make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 12 parts, Radix Scutellariae 16 parts, Rhizoma Imperatae 3 parts, 12 parts of Radix Glycyrrhizae, Radix Notoginseng 12 parts, Herba Agrimoniae 10 parts, 9 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 15 parts, Rhizoma Chuanxiong 15 parts, Radix Gentianae Macrophyllae 8 parts, Fructus Aurantii 5 parts, Rhizoma Acori Graminei 3 parts, Fructus Schisandrae Sphenantherae 14 parts, Herba Houttuyniae 9 parts, Herba Scutellariae Barbatae 6 parts, Rhizoma Cyperi 8 parts, Herba Rabdosiae glaucocalycis 5 parts, Cortex Mori 1 part, Herba Hedyotidis Diffusae 10 parts, Rhizoma Coptidis 5 parts, Rhizoma Polygoni Cuspidati 16 parts, Lignum Sappan 10 parts, Radix Osbeckiae Crinitae 8 parts, Rhizoma Sparganii 5 parts, Flos Ilicis Asprellae 1 part, Radix Puerariae 10 parts, 6 parts of Poria, the Radix Aucklandiae 8 parts, Fructus Gardeniae 3 parts, Herba Asari 15 parts, Herb Gynostemmae Pentaphylli 4 parts.

4. the pharmaceutical composition of suppression proliferation of human gastric cancer cell according to claim 1, it is characterized in that: make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 20 parts, Radix Scutellariae 10 parts, Rhizoma Imperatae 8 parts, 5 parts of Radix Glycyrrhizae, Radix Notoginseng 17 parts, Herba Agrimoniae 12 parts, 4 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 20 parts, Rhizoma Chuanxiong 10 parts, Radix Gentianae Macrophyllae 12 parts, Fructus Aurantii 2 parts, Rhizoma Acori Graminei 7 parts, Fructus Schisandrae Sphenantherae 11 parts, Herba Houttuyniae 15 parts, Herba Scutellariae Barbatae 3 parts, Rhizoma Cyperi 13 parts, Herba Rabdosiae glaucocalycis 3 parts, Cortex Mori 3 parts, Herba Hedyotidis Diffusae 5 parts, Rhizoma Coptidis 10 parts, Rhizoma Polygoni Cuspidati 13 parts, Lignum Sappan 3 parts, Radix Osbeckiae Crinitae 13 parts, Rhizoma Sparganii 3 parts, Flos Ilicis Asprellae 3 parts, Radix Puerariae 5 parts, 4 parts of Poria, the Radix Aucklandiae 10 parts, Fructus Gardeniae 4 parts, Herba Asari 13 parts, Herb Gynostemmae Pentaphylli 5 parts.

5. the pharmaceutical composition of suppression proliferation of human gastric cancer cell according to claim 1, it is characterized in that: make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 13 parts, Radix Scutellariae 11 parts, Rhizoma Imperatae 4 parts, 6 parts of Radix Glycyrrhizae, Radix Notoginseng 13 parts, Herba Agrimoniae 7 parts, 5 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 18 parts, Rhizoma Chuanxiong 11 parts, Radix Gentianae Macrophyllae 9 parts, Fructus Aurantii 4 parts, Rhizoma Acori Graminei 6 parts, Fructus Schisandrae Sphenantherae 12 parts, Herba Houttuyniae 13 parts, Herba Scutellariae Barbatae 4 parts, Rhizoma Cyperi 9 parts, Herba Rabdosiae glaucocalycis 4 parts, Cortex Mori 2 parts, Herba Hedyotidis Diffusae 6 parts, Rhizoma Coptidis 6 parts, Rhizoma Polygoni Cuspidati 14 parts, Lignum Sappan 5 parts, Radix Osbeckiae Crinitae 10 parts, Rhizoma Sparganii 4 parts, Flos Ilicis Asprellae 2 parts, Radix Puerariae 6 parts, 5 parts of Poria, the Radix Aucklandiae 12 parts, Fructus Gardeniae 3 parts, Herba Asari 12 parts, Herb Gynostemmae Pentaphylli 6 parts.

6. the pharmaceutical composition of suppression proliferation of human gastric cancer cell according to claim 1, it is characterized in that: make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 17 parts, Radix Scutellariae 15 parts, Rhizoma Imperatae 7 parts, 11 parts of Radix Glycyrrhizae, Radix Notoginseng 16 parts, Herba Agrimoniae 8 parts, 8 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 16 parts, Rhizoma Chuanxiong 14 parts, Radix Gentianae Macrophyllae 11 parts, Fructus Aurantii 3 parts, Rhizoma Acori Graminei 5 parts, Fructus Schisandrae Sphenantherae 13 parts, Herba Houttuyniae 14 parts, Herba Scutellariae Barbatae 5 parts, Rhizoma Cyperi 12 parts, Herba Rabdosiae glaucocalycis 3 parts, Cortex Mori 1 part, Herba Hedyotidis Diffusae 10 parts, Rhizoma Coptidis 9 parts, Rhizoma Polygoni Cuspidati 13 parts, Lignum Sappan 7 parts, Radix Osbeckiae Crinitae 11 parts, Rhizoma Sparganii 3 parts, Flos Ilicis Asprellae 1 part, Radix Puerariae 10 parts, 4 parts of Poria, the Radix Aucklandiae 11 parts, Fructus Gardeniae 4 parts, Herba Asari 14 parts, Herb Gynostemmae Pentaphylli 7 parts.

7. the pharmaceutical composition of suppression proliferation of human gastric cancer cell according to claim 1, it is characterized in that: make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 15 parts, Radix Scutellariae 12 parts, Rhizoma Imperatae 5 parts, 9 parts of Radix Glycyrrhizae, Radix Notoginseng 15 parts, Herba Agrimoniae 9 parts, 7 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 19 parts, Rhizoma Chuanxiong 13 parts, Radix Gentianae Macrophyllae 10 parts, Fructus Aurantii 4 parts, Rhizoma Acori Graminei 4 parts, Fructus Schisandrae Sphenantherae 11 parts, Herba Houttuyniae 11 parts, Herba Scutellariae Barbatae 5 parts, Rhizoma Cyperi 10 parts, Herba Rabdosiae glaucocalycis 5 parts, Cortex Mori 3 parts, Herba Hedyotidis Diffusae 8 parts, Rhizoma Coptidis 8 parts, Rhizoma Polygoni Cuspidati 14 parts, Lignum Sappan 9 parts, Radix Osbeckiae Crinitae 12 parts, Rhizoma Sparganii 5 parts, Flos Ilicis Asprellae 3 parts, Radix Puerariae 8 parts, 6 parts of Poria, the Radix Aucklandiae 9 parts, Fructus Gardeniae 5 parts, Herba Asari 12 parts, Herb Gynostemmae Pentaphylli 7 parts.

8. the pharmaceutical composition of suppression proliferation of human gastric cancer cell according to claim 1, it is characterized in that: make described pharmaceutical composition raw material composition and parts by weight be: Rhizoma Curcumae 19 parts, Radix Scutellariae 14 parts, Rhizoma Imperatae 6 parts, 7 parts of Radix Glycyrrhizae, Radix Notoginseng 14 parts, Herba Agrimoniae 9 parts, 6 parts of Fructus Amomi Rotundus, Radix Ophiopogonis 17 parts, Rhizoma Chuanxiong 13 parts, Radix Gentianae Macrophyllae 10 parts, Fructus Aurantii 4 parts, Rhizoma Acori Graminei 4 parts, Fructus Schisandrae Sphenantherae 11 parts, Herba Houttuyniae 11 parts, Herba Scutellariae Barbatae 5 parts, Rhizoma Cyperi 10 parts, Herba Rabdosiae glaucocalycis 5 parts, Cortex Mori 3 parts, Herba Hedyotidis Diffusae 8 parts, Rhizoma Coptidis 8 parts, Rhizoma Polygoni Cuspidati 14 parts, Lignum Sappan 4 parts, Radix Osbeckiae Crinitae 9 parts, Rhizoma Sparganii 5 parts, Flos Ilicis Asprellae 3 parts, Radix Puerariae 8 parts, 3 parts of Poria, the Radix Aucklandiae 8 parts, Fructus Gardeniae 5 parts, Herba Asari 15 parts, Herb Gynostemmae Pentaphylli 8 parts.

9. according to the arbitrary described pharmaceutical composition suppressing proliferation of human gastric cancer cell of claim 3-8, it is characterised in that: described pharmaceutical composition is prepared in following manner:

(1) each medical material of components by weight percent in the arbitrary described pharmaceutical composition of claim 3-8 is taken, add in the ethanol of 8-12 times amount and soak 1.5-2.5 hour, heating extraction 2 times, each 1.5-2 hour, removes supernatant, united extraction liquid, 120 orders filter, the ultrafiltration post ultrafiltration that molecular weight is 8000-12000 then through damming, the extractum of 1.3-1.4 when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C, heating is concentrated into paste, stand for standby use;

10. as described in as arbitrary in claim 1-9, suppress the application in suppressing proliferation of human gastric cancer cell medicine of the pharmaceutical composition of proliferation of human gastric cancer cell.