CN105663340A

CN105663340A - Antiviral oral liquid for animals and preparation method thereof

Info

Publication number: CN105663340A
Application number: CN201610101067.3A
Authority: CN
Inventors: 王林; 周玉武; 王涛; 王莹; 冉蓉; 杨建朋; 杨艳艳; 张敏
Original assignee: JINAN YIMIN ANIMAL MEDICINE CO Ltd
Current assignee: JINAN YIMIN ANIMAL MEDICINE CO Ltd
Priority date: 2016-02-24
Filing date: 2016-02-24
Publication date: 2016-06-15

Abstract

The invention relates to the technical field of veterinary drugs, particularly, the technical field of preparing antiviral veterinary medicines by utilizing symbiosis fermentation of multiple strains of probiotics, and in particular to antiviral oral liquid for animals and a preparation method of the antiviral oral liquid. The antiviral oral liquid for animals is prepared from traditional Chinese medicine components, lactic acid bacteria and yeast. According to the antiviral oral liquid disclosed by the invention, traditional veterinary herbal medicines and micro ecology are organically combined, the functions of reducing toxicity and enhancing the efficacy of the veterinary herbal medicines are realized, the unification of prevention, treatment and conditioning is realized, the dosage and the feeding cost are effectively reduced, and a new safe medicine is provided for green cultivation.

Description

A kind of antivirus veterinary oral liquid and its preparation method

Technical field

The present invention relates to veterinary drug technical field, especially utilize many probiotics symbiotic fermentation to prepare the technical field of antiviral veterinary drug, in particular to a kind of antivirus veterinary oral liquid and its preparation method.

Background technology

Virus is the important pathogenic agent of serious harm humans life and livestock industry safety, such as avian influenza virus, brings huge harm to the economic security of China and social stability. Western medicine antiviral drug is oral substantially invalid, and herbal medicine then has good effect in prevention and cure of viruses and control. But the problems such as tradition herbal medicine also exists, and consumption is big, absorption is slow, it is slow to take effect, cost height, bioavailability are low, seriously constrain development and the application of herbal medicine.

Herb fermenting, as one of Chinese medicine preparation working method, has one page of its radiance in China's Chinese medicine development history. Modern Chinese herbal medicine fermentation technique is based on traditional Chinese medicine preparation fermentation method, utilize the singularity of microecology, and in conjunction with a kind of important high-tech pharmacy novel process that zymetology engineering produces, in herb fermenting process, microorganism plays powerful decomposition and inversion ability, create multiple secondary metabolite, by a relatively large margin change the property of medicine, reduce toxic side effect, improve curative effect, produce new drug effect, be beneficial to absorption, improve palatability etc.

Using probiotics fermention Chinese medicine, probiotic bacterium both can have been utilized to be transformed by Chinese medicine, it is to increase curative effect, simultaneously probiotic bacterium and meta-bolites thereof have to have and improve intestines and stomach environment, suppress the function such as pathogenic bacteria, strengthening immunity. Probiotic bacterium and Chinese medicine are organically combined, both can play the effect of prevention and therapy, the effect of conditioning can be played again, reach the effect of synergy.

At present, in this technical field, there is no effective veterinary drug.

Summary of the invention

In order to solve the problem of prior art, the present invention provides a kind of antivirus veterinary oral liquid and its preparation method, the antivirus veterinary oral liquid that it is produced have antiviral effect significantly, bioavailability height, have the feature improving animal body environment, conditioning intestines and stomach, strengthening immunity concurrently.

The technical solution adopted in the present invention is as follows:

A kind of antivirus veterinary oral liquid, by traditional Chinese medicinal components, milk-acid bacteria and yeast composition, wherein, described traditional Chinese medicinal components comprises Root of Indigowoad, Japanese Honeysuckle, the capsule of weeping forsythia, cape jasmine, the Radix Astragali, echinacea purpurea, taraxacum, plaster stone, Perilla Leaf and Root of Chinese Pulsatilla, described milk-acid bacteria is one or more in Lactobacterium acidophilum, plant lactobacillus, enterococcus faecalis, faecium, and described yeast is one or both in Candida utilis, yeast saccharomyces cerevisiae.

The preparation method of antivirus veterinary oral liquid, comprises the following steps:

(1) traditional Chinese medicinal components is extracted: pulverize after Root of Indigowoad 0-20g, Japanese Honeysuckle 0-10g, capsule of weeping forsythia 0-5g, cape jasmine 0-5g, Radix Astragali 0-10g, echinacea purpurea 0-10g, taraxacum 0-5g, plaster stone 0-20g, Perilla Leaf 0-5g, Root of Chinese Pulsatilla 0-5g being mixed, add 6～8 times of soak by water 3 times, each 2 hours, after collecting three filtrates, merge the concentrated Chinese medicine extract obtaining Chinese medicine/water=1g/mL;

(2) probiotic's culture: get frozen milk-acid bacteria, yeast bacterial classification respectively, be inoculated in seed culture medium, cultivates 18 hours, obtains the seed liquor of bacterial content >=1 × 109/mL for 37 DEG C;

(3) Traditional Chinese medicine probiotic fermentation: by described Chinese medicine extract, adopt 121 DEG C of sterilizing 20min, add the seed liquor of preculture by the inoculum size of 5% in the Chinese medicine extract after sterilizing, and under 28 DEG C～37 DEG C constant temperature fermentation culture 24 hours～60 hours, pH is down to and terminates fermentation after below 4.0, fermented liquid is directly sealed filling, obtain described antivirus veterinary oral liquid.

The using method of antivirus veterinary oral liquid, is add in the ratio of 0.1%～0.2% in drinking-water or feed, uses 7-10 day continuously. Or continuous use 3-5 day in drinking-water or feed is added in the ratio of 1%～2%.

Antivirus veterinary oral liquid prepared by a kind of many probiotics symbiotic fermentation of the present invention, by traditional Chinese medicinal components, the probiotic bacterium such as milk-acid bacteria, yeast and probiotic culture etc. form.

Traditional Chinese medicinal components extracts the compound of following medicinal material composition: Root of Indigowoad 0-20 mass parts, Japanese Honeysuckle 0-10 mass parts, capsule of weeping forsythia 0-5 mass parts, cape jasmine 0-5 mass parts, Radix Astragali 0-10 mass parts, echinacea purpurea 0-10 mass parts, taraxacum 0-5 mass parts, plaster stone 0-20 mass parts, Perilla Leaf 0-5 mass parts, Root of Chinese Pulsatilla 0-5 mass parts. This compound itself has stronger clearing heat and detoxicating effect.

Antivirus oral liquid prepared by a kind of many probiotics symbiotic fermentation of the present invention, milk-acid bacteria is one or more in Lactobacterium acidophilum, plant lactobacillus, enterococcus faecalis, faecium, and described yeast is one or more in Candida utilis, yeast saccharomyces cerevisiae. Bacterial strain used is in fodder additives catalogue to allow the probiotic bacterium used, identical with the main probiotics class in animal intestinal. There is between described milk-acid bacteria and yeast good symbiotic relationship, co-cultivation can obtain higher viable count than single culture, closer to the micro-ecological environment of animal intestinal, use probiotic bacterium to imitate intestinal environment and Chinese medicine is carried out microbe conversion, the bioavailability of effective ingredient can be significantly improved, strengthen drug effect.

The antivirus oral liquid prepared by above step, finds: the probiotic activity in oral liquid is stronger by analysis, it is possible to the sour environment of tolerance pH2.5, it is possible to tolerate the bovine bile of 0.5% concentration, and normal temperature preserves viable count decline≤5% in 6 months; Traditional Chinese medicinal components in oral liquid finds through thin-layer chromatography and efficient liquid phase chromatographic analysis, some new activeconstituentss have occurred, part active component content increases obviously, find through gel chromatography analysis, lower molecular weight scope material concentration increases obviously, the material of part high molecular scope disappears, above result shows, through microbial transformation, active ingredient in Chinese medicine there occurs obvious change, creating new activeconstituents, the degraded of part macromolecular substance, in small-molecule substance, more is conducive to absorbing.

The antivirus oral liquid of gained adds continuous use 7-10 day in drinking-water or feed in the ratio of 0.1%～0.2%, or continuous use 3-5 day in drinking-water or feed is added in the ratio of 1%～2%, there is good heat-clearing toxin-expelling functions, can effectively prevention and therapy such as diseases such as influenza, newcastle disease, the fabricius bursa, bronchitis, glandular stomach inflammation, inclusion body hepatitis.

The usefulness of the present invention is:

(1) inventive formulation is simple, and cost is low, and after being transformed by probiotic bacterium, effective constituent is more easily absorbed by animal, and treatment and preventive effect improve more than 50%;

(2) milk-acid bacteria and yeast are while transforming Chinese medicine, and its thalline and meta-bolites enter after in animal body, it is also possible to play and improve intestines and stomach environment, the suppression effect such as pathogenic bacteria, strengthening immunity, it is achieved prevention and the effect nursed one's health after being ill before sick;

(3) antivirus oral liquid that prepared by many probiotics symbiotic fermentation provided by the invention is without any side effects, can add in daily feed and drinking-water and use, play the effect of long-term care and prevention.

Tradition herbal medicine and Tiny ecosystem are organically combined by the present invention, by the conversion of probiotic bacterium, it is achieved the effect of the attenuation synergistic of herbal medicine, it is achieved prevention, treatment and conditioning are unified, effectively reduce dosage and feeding cost, for green cultivation provides a kind of new safe drugs.

Embodiment

For making the object, technical solutions and advantages of the present invention clearly, it is described in further detail below in conjunction with embodiment.

Embodiment one

The present embodiment provides the Chinese medicine extract of a kind of antivirus veterinary oral liquid:

(1) Chinese medicinal materials is taken: Root of Indigowoad 10g, Japanese Honeysuckle 5g, capsule of weeping forsythia 5g, cape jasmine 5g, Radix Astragali 10g, echinacea purpurea 8g, taraxacum 5g, plaster stone 20g, Perilla Leaf 5g, Root of Chinese Pulsatilla 5g.

(2), after being pulverized by Chinese medicinal materials, times soak by water that adds 83 times, each 2h, after collecting three filtrates, merges and concentrates to 1g (crude drug)/mL, obtain without the Chinese medicine extract fermented.

Embodiment two

The present embodiment provides a kind of antivirus veterinary oral liquid:

(1) Root of Indigowoad 10g, Japanese Honeysuckle 5g, capsule of weeping forsythia 5g, cape jasmine 5g, Radix Astragali 10g, taraxacum 5g, plaster stone 20g, Perilla Leaf 5g, Root of Chinese Pulsatilla 5g is taken.

(2) after Chinese medicinal materials being pulverized, times soak by water that adds 83 times, each 2h, after collecting three filtrates, merging concentrates to 1g (crude drug)/mL;

(3) Chinese medicine extract 121 DEG C of sterilizing 20min;

(4) access frozen Lactobacterium acidophilum, plant lactobacillus and Candida utilis bacterial classification after seed culture medium sterilizing, cultivate 18h, obtain seed liquor for 37 DEG C;

(5) adding, in the extracting solution after sterilizing, the seed liquor cultivated by the inoculum size of 5%, 37 DEG C of constant temperature culture 48h, pH are down to 3.85, terminate fermentation, are directly sealed by fermented liquid filling, obtain a kind of antivirus veterinary oral liquid of the present embodiment.

Embodiment three:

The present embodiment provides another antivirus veterinary oral liquid:

(1) Root of Indigowoad 8g, Japanese Honeysuckle 5g, cape jasmine 5g, Radix Astragali 10g, echinacea purpurea 5g, taraxacum 5g, Root of Chinese Pulsatilla 5g is taken.

(2) after Chinese medicinal materials being pulverized, times soak by water that adds 63 times, each 2h, after collecting three filtrates, merging concentrates to 1g (crude drug)/mL;

(3) Chinese medicine extract adopts 121 DEG C of sterilizing 20min;

(4) access frozen Lactobacterium acidophilum, plant lactobacillus, enterococcus faecalis, faecium, yeast saccharomyces cerevisiae and Candida utilis bacterial classification after seed culture medium sterilizing, cultivate 18h at 37 DEG C, obtain seed liquor;

(5) adding, in the Chinese medicine extract after sterilizing, the seed liquor cultivated by the inoculum size of 5%, 37 DEG C of constant temperature culture 24h, pH are down to 3.95, terminate fermentation, are directly sealed by fermented liquid filling, obtain a kind of antivirus veterinary oral liquid of the present embodiment.

Embodiment four:

The present embodiment provides another antivirus veterinary oral liquid:

(1) Root of Indigowoad 20g, Japanese Honeysuckle 10g, capsule of weeping forsythia 2g, cape jasmine 2g, taraxacum 3g, plaster stone 20g, Perilla Leaf 2g is taken;

(3) Chinese medicine extract 121 DEG C of sterilizing 20min;

(4) access frozen plant lactobacillus, enterococcus faecalis and yeast saccharomyces cerevisiae bacterial classification after seed culture medium sterilizing, cultivate 18h, obtain seed liquor for 37 DEG C;

(5) adding, in the Chinese medicine extract after sterilizing, the seed liquor cultivated by the inoculum size of 5%, 37 DEG C of constant temperature culture 60h, pH are down to 3.90, terminate fermentation, are directly sealed by fermented liquid filling, to obtain final product.

The antivirus veterinary oral liquid that above embodiment obtains, is occurring on the basis of pathological model of newcastle disease by manually manufacturing broiler chicken, and application antivirus oral liquid prevents, and achieves satisfied effect. For this reason, by 108 age on the 30th white meat-type chickens be divided into 3 groups at random, often organize 36. A group is that antivirus oral liquid (embodiment 2) prepared by many probiotics symbiotic fermentation drinks group, and B group is Chinese medicine extract (embodiment 1) group, and C group is blank group. From age on the 14th, A group and B group by 0.2% amount of drinking water administration, it is used in conjunction 7 days, C group not administration. When age on the 25th, three components did not carry out intramuscular injection to cause the pathological model of the sick chicken of newcastle disease with the NDVI system vaccine of 10 times amount.

1, HI antibody titer after each experimental group NDV vaccine intramuscular injection the results are shown in Table 1

Change (nlog2) (X ± S) of the anti-NDV antibody titer of table 1 different days chicken serum

Age day	A	B	C
				13	2.570±0.152	2.800±0.200	2.330±0.100
21	2.410±0.100	2.680±0.300	2.610±0.200
				32	4.810±0.250	4.150±0.100	3.530±0.100

As seen from the results in Table 1, when age on the 13rd and 21,3 groups of antibody horizontal difference little (P > 0.05), but during age on the 32nd, A group antibody horizontal is obviously higher than B, C group, significant difference (P < 0.05), shows that antivirus oral liquid prepared by probiotic bacterium symbiotic fermentation can strengthen the humoral immunization ability of chicken.

2, each sickness rate and mortality ratio organizing chicken after attacking poison

As seen from the results in Table 2, the morbidity number of A group and B group and death toll are far below C group, and wherein A group is without dead example, morbidity example less and is recovered rapidly, show that antivirus oral liquid prepared by probiotic bacterium symbiotic fermentation can significantly improve the anti-virus ability of chicken group, reduce morbidity number and death toll, accelerate resume speed.

After poison attacked by table 2, chicken falls ill and death condition

Applicating example

1, one, Shandong province Accessories during Binzhou breeding layer chicken Specialty Co-operative Organization, in plant, one batch of laying hen occurs that body temperature raises, and spirit is depressed, feather is fluffy and disorderly, and feed consumption reduces, and egg productivity obviously reduces or do not lay eggs, ight soil is that yellow-green colour is just rare, cockscomb swelling is hemorrhage, dissects and finds tracheorrhagia, adds the antivirus oral liquid of the embodiment of the present invention 4 in drinking-water according to the ratio of 1%, use 3 days continuously, drinking-water is searched for food increases, and ight soil recovers normal, and egg productivity is also recovered normally gradually.

2, broiler breeding Specialty Co-operative Organization of one, Weifang City of province, Shandong, from broiler chicken age on the 6th, drinking-water adds the antivirus oral liquid of the embodiment of the present invention 3 according to the ratio of 0.1%, use 8 days continuously, use group is not compared with using group, average food consumption is high by 10%～15%, and mean body weight is high by 15%～25%, diarrhoea sickness rate of Denging is low by 60%～80%.

3, enterprise of raising pigs of one, Kaifeng, Henan Province city, one batch of weanling pig food consumption decline, cessation of growth cessation, diarrhoea, adds the antivirus oral liquid of the embodiment of the present invention 4 in drinking-water according to the ratio of 2%, uses 5 days continuously, drinking-water is searched for food increases, and ight soil recovers normal, and growth is normally.

The foregoing is only the better embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment of doing, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims

1. an antivirus veterinary oral liquid, by traditional Chinese medicinal components, milk-acid bacteria and yeast composition, wherein, described traditional Chinese medicinal components comprises Root of Indigowoad, Japanese Honeysuckle, the capsule of weeping forsythia, cape jasmine, the Radix Astragali, echinacea purpurea, taraxacum, plaster stone, Perilla Leaf and Root of Chinese Pulsatilla, described milk-acid bacteria is one or more in Lactobacterium acidophilum, plant lactobacillus, enterococcus faecalis, faecium, and described yeast is one or both in Candida utilis, yeast saccharomyces cerevisiae.

2. the preparation method of an antivirus veterinary oral liquid as claimed in claim 1, it is characterised in that, comprise the following steps:

3. a using method for antivirus veterinary oral liquid as claimed in claim 1 or 2, is add in the ratio of 0.1%～0.2% in drinking-water or feed, uses 7-10 day continuously.

4. a using method for antivirus veterinary oral liquid as claimed in claim 1 or 2 adds in the ratio of 1%～2% to use 3-5 day in drinking-water or feed continuously.