CN105639383A - Crocodile drink capable of enhancing immunity - Google Patents
Crocodile drink capable of enhancing immunity Download PDFInfo
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- CN105639383A CN105639383A CN201610029282.7A CN201610029282A CN105639383A CN 105639383 A CN105639383 A CN 105639383A CN 201610029282 A CN201610029282 A CN 201610029282A CN 105639383 A CN105639383 A CN 105639383A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention provides a crocodile drink capable of enhancing immunity. The crocodile drink is prepared from crocodile meat, semen gingko, bulbus lilii, Chinese wolfberry fruits, radix puerariae and purified water. The mentioned formula further comprises one or more of essence, citric acid, sodium citrate, xylitol or nisin as an additive/additives. According to the crocodile drink, crocodile meat is adopted as the main raw material, and such Chinese herbal medicine as radix puerariae is added, so that the crocodile drink is processed and obtained, all effects supplement with one another, the efficacy of crocodile meat can be achieved to the greatest extent, the production method is simple and easy to conduct, and organism immunity can be better enhanced.
Description
Technical field
The invention belongs to health product technology field, particularly to a kind of health promoting beverage with good enhancing human body immunity power effect being main component with crocodile meat, specifically a kind of crocodile beverage with enhancing immunity effect.
Background technology
Crocodile is the most ancient reptile, has survived on earth more than 200,000,000 3 thousand ten thousand years, thus has had the title of " living fossil ". Many ancient medical books once had the record that crocodile is medicinal, wherein be expressly recited in Compendium of Material Medica: " Chinese alligator (i.e. crocodile) first: cure mainly trusted subordinate's lump in the abdomen; Fu Jian gathers; cold and heat; phase dragging pain in woman's lower abdomen the moon, the metrorrhagia hematochezia five colors and the dead flesh of skin ulcer scabies ... meat: cure mainly few aspiration and inhale; foot not on the spot, not Lu dampness pathogen, all legendary venomous insects, abdominal mass, malignant boil in abdomen; Fat: cure mainly rub wind and malignant boil ... ". It addition, " medical center bun will " records Carnis Alligator " can use proud cream, routed heavily fortified point is drawn out pus by applying a plaster to the affected part, expelling the stasis for the new ".
Crocodile meat delicious flavour, it is high protein, low fat, low cholesterol the gourmet food containing multivitamin and trace element and tonic, also there is effect for the treatment of gastric ulcer and asthma, be equipped with Chinese medicine and use, also respiratory tract neoplasms is had clear and definite therapeutical effect.
Modern scientific research finds, the mice constitution and health level raised with Chinese alligator meat increases, and ability of learning and memory is strengthened, and the tolerance of noxious stimulation is strengthened. Beverage is consumer food maximum beyond staple food, is equipped with Chinese medicine ingredients with crocodile meat for primary raw material and makes beverage class health product, will have huge market potential.
Summary of the invention
In view of this, the invention is directed to a kind of crocodile beverage with enhancing human body immunity power effect with crocodile meat for main component simultaneously other Chinese medicine ingredients of compatibility, quotes demand with what meet society.
For reaching above-mentioned purpose, the technical scheme of the invention is achieved in that
A kind of crocodile beverage with enhancing immunity effect, its formula contains following components: crocodile meat, Semen Ginkgo, Bulbus Lilii, Fructus Lycii, Radix Puerariae and pure water.
Further, above-mentioned formula also comprises at least one in essence, citric acid, sodium citrate, xylitol or nisin as additive.
Further, crocodile meat 30-40 part, Semen Ginkgo 5-10 part, Bulbus Lilii 10-15 part, Fructus Lycii 10-15 part, Radix Puerariae 5-10 part, pure water 1000-2000 part.
Further, crocodile meat 30 parts, Semen Ginkgo 5 parts, Bulbus Lilii 10 parts, Fructus Lycii 10 parts, Radix Puerariae 5 parts, pure water 2000 parts.
Further, described crocodile beverage has effect of enhancing human body immunity power.
The preparation method that it is a further object of the present invention to provide a kind of crocodile beverage, described method is:
(1) crocodile meat of formula ratio is removed oils and fats, after breaking into meat slurry, add neutral protease enzymolysis;
(2) enzymolysis solution filters to obtain supernatant 1 after inactivateing then through water bath with thermostatic control;
(3) separately take the Semen Ginkgo of formula ratio, Bulbus Lilii, Fructus Lycii, Radix Puerariae water carry 2��3 times, merging filtrate after filtration, and precipitation refilters, and obtains supernatant 2;
(4) taking supernatant 1 to mix with supernatant 2, add the pure water preparation of formula ratio, regulating PH is 6��7, obtains mixing supernatant 3;
(5) after adding formula ratio additive in gained mixing supernatant 3 in step (4), filtration after cryopreservation, subpackage, sterilizing or direct by filtration, subpackage, sterilizing after gained mixing supernatant 3 cryopreservation in step (4).
Further, the enzymatic hydrolysis condition in described step (1) is the neutral protease adding 5%, 37 DEG C of insulation enzymolysis 8h.
Further, the inactivation condition of the enzymolysis solution in described step (2) is 85 DEG C of water bath with thermostatic control inactivation 20min.
Further, it is each 2 hours that the water in described step (3) puies forward process, and it is 8 hours that water carries the sedimentation time after filtration.
Further, in described step (5), the cryopreservation time is 24h, and sterilized environment is 121 DEG C, 0.11MPa sterilizing 30min.
Relative to prior art, the crocodile beverage described in the invention has the advantage that
Crocodile beverage of the present invention adopts crocodile meat to be primary raw material, and add the crocodile beverage that the Chinese herbal medicine such as Radix Puerariae are processed into, various effects are complementary so that effect of crocodile meat obtains maximum performance, manufacture method is simple, it is possible to better enhancing human body immunity power.
Detailed description of the invention
Unless otherwise indicated, term used herein is respectively provided with the implication that those skilled in the art's routine is understood, and for the ease of understanding the present invention, terms more used herein has been carried out following definitions.
Using in the specification and in the claims, odd number type " " and " this " include plural reference, clearly state unless the context otherwise. Such as, term " (one) cell " includes the cell of plural number, including its mixture.
All of Digital ID, for instance pH, temperature, time, concentration, including scope, is all approximation. It is to be understood that, although all add term " about " before all of Digital ID of always not clear and definite narration. Simultaneously it will also be understood that, although always not clear and definite narration, reagent described herein is only example, and its equivalent is known in the art.
The invention is described in detail below in conjunction with embodiment and coherent detection data.
Wherein, in embodiment, test material used and source thereof include:
1. animal:
Male mouse of kunming 60, body weight 18��20g, to raise in SPF rank Mice Residence, laboratory animal is provided by National Institute for Food and Drugs Control, the quality certification number: NO.11400500007331.
2. Experimental agents and reagent
Crocodile beverage: liquid, is provided by Bai Shengkang bio tech ltd, Beijing.
Crocodile meat: provided by Bai Shengkang bio tech ltd, Beijing.
Dinitrofluorobenzene (DNFB): purchased from sigma company, article No.: D1529, purity >=99%, specification 10mL.
India ink: purchased from Suo Laibao company, article No.: 18060, specification 100mL.
3. key instrument
Ultraviolet spectrophotometer: Japan's Shimadzu, model: UV-2201;
Electronic balance: Shanghai Ao Haosi Instrument Ltd., model: AR2202CN;
Effect of the enhancing immunity of the crocodile beverage of the invention is described in detail below in conjunction with embodiment and different experiments example and coherent detection data.
Embodiment 1
A kind of crocodile beverage with enhancing immunity effect, the described crocodile beverage packets component containing following weight portion: crocodile meat 30 parts, Semen Ginkgo 5 parts, Bulbus Lilii 10 parts, Fructus Lycii 10 parts, Radix Puerariae 5 parts, pure water 1000, is additionally supplemented with xylitol 5 parts, nisin 0.05 part.
The preparation method of described crocodile beverage is: first crocodile meat is removed oils and fats, breaks into meat slurry, adds 5% neutral protease, 37 DEG C of insulation enzymolysis 8h, and enzymolysis solution goes to 85 DEG C of water bath with thermostatic control inactivation 20min, filters to obtain supernatant 1; Separately taking, Semen Ginkgo, Bulbus Lilii, Fructus Lycii, Radix Puerariae water carry twice, each 2 hours, filter, merging filtrate, precipitate 8 hours, filter to obtain supernatant 2; Taking supernatant 2 and crocodile meat enzymolysis solution (i.e. supernatant 1) mixing, purified water is prepared, and adjusting PH is 6.8, obtains mixing supernatant 3, adds xylitol, nisin, low temperature 24h, filters. It is distributed into 30ml/ bottle, 121 DEG C, 0.11MPa sterilizing 30min.
The immunoregulation effect of experimental example 1 ear swelling method inspection crocodile beverage
(1) raising of animal mice and crocodile beverage feeding process
Experiment mice is raised in SPF level receptacle, and 30 body weight are randomly divided into 3 groups at the kunming mice of 18��20, often group 10, respectively matched group, crocodile beverage group, crocodile meat enzymolysis solution group. Wherein, the content of crocodile meat enzymolysis solution (i.e. supernatant 1) concentration crocodile meat enzymolysis solution contained with crocodile beverage is consistent. Animal arrives and starts to give the process of mice crocodile beverage feeding after SPF level receptacle stablizes 3 days, allows every day mice drink crocodile beverage and each 30mL of crocodile meat enzymolysis solution, control group mice drinking pure 30mL, persistently feeds 30 days.
(2) DNFB sensitized mice
DNFB solution preparation: weigh 50mgDNFB, it is placed in clean dry bottle, takes pre-configured acetone sesame oil solution (acetone: Oleum Sesami=1:1) 5ml and add bottle, build bottle stopper blend compounds cloth and seal, after mixing, taken by bottle cap with 250 �� L syringes.
Sensitization: the skin of abdomen of every mice is carried out depilation process with barium sulfide, lose hair or feathers scope 3cm �� 3cm, then takes 50 �� LDNFB and is uniformly applied to depilation place, sensitization.
Sensitization post processing: sensitization, after 5 days, is attacked in mouse right ear (two sides) with 10 �� LDNFB solution uniform application, attacks latter 24 hours cervical dislocation and puts to death mice, cut left and right auricular concha, take off, with card punch, the auricle that diameter is 8mm, weigh. Statistics ear tuple evidence, and compare group difference, result is in Table 1.
Table 1 given the test agent is to mice delayed allergy result (x �� s)
(3) interpretation of result:
As shown in table 1, compared with matched group, the mice ear degree drinking crocodile beverage and crocodile meat enzymolysis solution every day all decreases, and the mice ear degree reduction level drinking crocodile beverage group is better than crocodile meat enzymolysis solution group mice, and there is significant difference with matched group.
Mouse macrophage is swallowed merit by experimental example 2 mice carbonic clearance experimental check crocodile beverage
The impact of energy
(1) raising of animal mice and crocodile beverage feeding process
Experiment mice is raised in SPF level receptacle, and 30 body weight are randomly divided into 3 groups at the kunming mice of 18��20, often group 10, respectively matched group, crocodile beverage group, crocodile meat enzymolysis solution group.Wherein, the amount of crocodile meat hydrolyzate content crocodile meat enzymolysis solution contained with crocodile beverage is consistent. Animal arrives and starts to give the process of mice crocodile beverage feeding after SPF level receptacle stablizes 3 days, allows every day mice drink crocodile beverage and each 30mL of crocodile meat enzymolysis solution, control group mice drinking pure 30mL, persistently feeds 30 days.
(2) carbonic clearance detection
After mice is administered 30 days, it is injected intravenously the india ink of 3��4 times of dilutions from tail according to Mouse Weight (100ml/kg), timing immediately after prepared Chinese ink injects. 2min and 10min after injection prepared Chinese ink, takes blood 20 �� L with hemoglobin pipet from angular vein clump respectively, exists side by side and be namely added into 2mL0.1%Na2In CO3 solution. With the optical density value (OD) under 721 spectrophotometric determination 600nm wavelength, Na2CO3 solution is as blank.
After angular vein takes blood, put to death mice, take mouse liver and spleen, after blotting organ surface remained blood with filter paper, weigh respectively.
The calculating of phagocytic index: the phagocytic index of mice represents mice carbonic clearance ability. The phagocytic index of administration group mice, apparently higher than normal group, can determine that as experimental result positive.
Computing formula is:
Result of calculation is as follows:
Table 2 given the test agent is to mice carbonic clearance experimental result (x �� s)
As shown in table 2, compared with matched group, the blood carbonic clearance ability drinking crocodile beverage and crocodile meat enzymolysis solution mice all strengthens to some extent, and crocodile beverage group effect is better than crocodile meat enzymolysis solution group, obvious difference compared with matched group, has statistical significance.
Above-mentioned experiments show that, crocodile beverage of the present invention adopts crocodile meat to be primary raw material, and add the crocodile beverage that the Chinese herbal medicine such as Radix Puerariae are processed into, various effects are complementary, the effect making crocodile meat obtains maximum performance, manufacture method is simple, it is possible to better enhancing human body immunity power, is suitable for big commercial production and popularization.
The foregoing is only the preferred embodiment of the invention; not in order to limit the invention; within all spirit in the invention and principle, any amendment of making, equivalent replacement, improvement etc., should be included within the protection domain of the invention.
Claims (10)
1. a crocodile beverage with enhancing immunity effect, it is characterised in that its formula contains following components: crocodile meat, Semen Ginkgo, Bulbus Lilii, Fructus Lycii, Radix Puerariae and pure water.
2. crocodile beverage according to claim 1, it is characterised in that also comprise at least one in essence, citric acid, sodium citrate, xylitol or nisin in above-mentioned formula as additive.
3. crocodile beverage according to claim 1, it is characterised in that by weight, its each constituent content is as follows: crocodile meat 30-40 part, Semen Ginkgo 5-10 part, Bulbus Lilii 10-15 part, Fructus Lycii 10-15 part, Radix Puerariae 5-10 part, pure water 1000-2000 part.
4. the crocodile beverage according to claim 1 or 3, it is characterised in that: its each constituent content is as follows: crocodile meat 30 parts, Semen Ginkgo 5 parts, Bulbus Lilii 10 parts, Fructus Lycii 10 parts, Radix Puerariae 5 parts, pure water 2000 parts.
5. crocodile beverage according to claim 1, it is characterised in that: described crocodile beverage has effect of enhancing human body immunity power.
6. the preparation method of crocodile beverage according to claim 1, it is characterised in that described method is:
(1) crocodile meat of formula ratio is removed oils and fats, after breaking into meat slurry, add neutral protease enzymolysis;
(2) enzymolysis solution filters to obtain supernatant 1 after inactivateing then through water bath with thermostatic control;
(3) separately take the Semen Ginkgo of formula ratio, Bulbus Lilii, Fructus Lycii, Radix Puerariae water carry 2��3 times, merging filtrate after filtration, and precipitation refilters, and obtains supernatant 2;
(4) taking supernatant 1 to mix with supernatant 2, add the pure water preparation of formula ratio, regulating PH is 6��7, obtains mixing supernatant 3;
(5) after adding formula ratio additive in gained mixing supernatant 3 in step (4), filtration after cryopreservation, subpackage, sterilizing or direct by filtration, subpackage, sterilizing after gained mixing supernatant 3 cryopreservation in step (4).
7. the preparation method of crocodile beverage according to claim 6, it is characterised in that the enzymatic hydrolysis condition in described step (1) is the neutral protease adding 5%, 37 DEG C of insulation enzymolysis 8h.
8. the preparation method of crocodile beverage according to claim 6, it is characterised in that the inactivation condition of the enzymolysis solution in described step (2) is 85 DEG C of water bath with thermostatic control inactivation 20min.
9. the preparation method of crocodile beverage according to claim 6, it is characterised in that it is each 2 hours that the water in described step (3) puies forward process, it is 8 hours that water carries the sedimentation time after filtration.
10. the preparation method of crocodile beverage according to claim 6, it is characterised in that in described step (5), the cryopreservation time is 24h, sterilized environment is 121 DEG C, 0.11MPa sterilizing 30min.
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