CN105639117A - Method for producing protein feed by use of flat peach skin dregs - Google Patents
Method for producing protein feed by use of flat peach skin dregs Download PDFInfo
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- CN105639117A CN105639117A CN201610001683.1A CN201610001683A CN105639117A CN 105639117 A CN105639117 A CN 105639117A CN 201610001683 A CN201610001683 A CN 201610001683A CN 105639117 A CN105639117 A CN 105639117A
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- peento
- skin slag
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- peach skin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a method for producing a protein feed by use of flat peach skin dregs. The method comprises the following steps: 1) performing composite enzymatic hydrolysis on the flat peach skin dregs so as to obtain flat peach skin dregs subjected to composite enzymatic hydrolysis; and 2) preparing a fermentation medium by use of the flat peach skin dregs subjected to composite enzymatic hydrolysis in the step 1, inoculating composite strains, and fermenting to obtain a crude protein feed. The method for producing the protein feed by use of the flat peach skin dregs, disclosed by the invention, has the beneficial effects of screening optimal enzymes, namely cellulose and pectinase, for degrading the flat peach skin dregs, determining technological parameters of composite enzymatic hydrolysis, further determining technological conditions for processing the flat peach skin dregs into a microbial protein feed, utilizing the waste flat peach skin dregs as raw materials, performing a fermentation experiment by use of multiple types of lactobacillus, turning wastes into valuable things and realizing the reprocessing and reutilization of the flat peach skin dregs.
Description
Technical field
The present invention relates to albumen feedstuff production technical field, be specifically related to a kind of method utilizing peento skin slag to produce albumen feedstuff.
Background technology
Peento, formal name used at school honey peach, originate in Xinjiang, have won fame both at home and abroad with features such as its shape U.S., color is gorgeous, taste is good, meat is thin, Pi Ren is easy-peel, the how sweet thickness of juice, taste giving off a strong fragrance overflow, just melt in the mouths. Peento is excellent in color not only, and containing certain protein, fat, vitamin and several mineral materials, annual export trade port, Australia and abroad reach more than hundred tons, is subject to the favor of people. There is a large amount of flat peach wine manufacturer in Xinjiang, and in recent years, the deep processed product of peento gets more and more, and needs reject skin and slag so that the output of peento skin slag grows with each passing day, cause waste and the environmental pollution of peento resource in the flat peach wine course of processing. Meanwhile, Xinjiang Animal Husbandry development is swift and violent, and feedstuff shortage particularly produces the resource shortage of albumen feedstuff, is always up the great difficult problem that Xinjiang region is encountered.
Summary of the invention
The purpose of the present invention is aiming at above-mentioned defect of the prior art, it is provided that a kind of method utilizing peento skin slag to produce albumen feedstuff.
To achieve these goals, technical scheme provided by the invention is: a kind of method utilizing peento skin slag to produce albumen feedstuff, comprises the following steps:
1) peento skin slag is carried out complex enzyme hydrolysis, obtain peento skin slag after complex enzyme hydrolysis;
2) prepare fermentation medium with peento skin slag after the complex enzyme hydrolysis that step 1) obtains for major ingredient, access combination bacterial strain and carry out fermentation and prepare crude protein feed.
Further, a kind of above-mentioned method utilizing peento skin slag to produce albumen feedstuff, in described step 1), peento skin slag is the leftover bits and pieces produced in the flat peach wine course of processing.
Further, a kind of above-mentioned method utilizing peento skin slag to produce albumen feedstuff, in described step 1), compound enzyme used by complex enzyme hydrolysis is cellulase and pectase.
Further, a kind of above-mentioned method utilizing peento skin slag to produce albumen feedstuff, in described step 1), complex enzyme hydrolysis condition is: cellulase consumption 68mg/g, pectase consumption 20mg/g, and temperature is 40 DEG C, pH4.5, and the time is 4h;After complex enzyme hydrolysis, content of reducing sugar is 4.69%.
Further, a kind of above-mentioned method utilizing peento skin slag to produce albumen feedstuff, described step 2) in, consisting of of fermentation medium: peento skin slag 88 parts in parts by weight, sunflower seed dregs 9 parts, 1 part of wheat bran, 1 part of carbamide, 1 part of ammonium sulfate; In described fermentation medium, moisture is that 64%-66%, pH are natural.
Further, a kind of above-mentioned method utilizing peento skin slag to produce albumen feedstuff, described step 2) in, described combination bacterial strain is candidiasis, aspergillus oryzae and Lactobacillus plantarum.
Further, a kind of above-mentioned method utilizing peento skin slag to produce albumen feedstuff, described step 2) in, fermentation condition is: inoculum concentration is 10% altogether, and inoculative proportion is 1:1:1, and fermentation time is 3d, and fermentation temperature is 32 DEG C; After fermentation, crude protein content is 38.96%.
The invention have the benefit that a kind of method utilizing peento skin slag to produce albumen feedstuff provided by the invention, waste situation mainly for Xinjiang characteristic resource peento skin slag (leftover bits and pieces produced in the flat peach wine course of processing), swift and violent in conjunction with Xinjiang Animal Husbandry development, the present situation that albumen feedstuff lacks, with peento skin slag for major ingredient, utilize traditional solid fermentation mode, produce crude protein content height, meet the albumen feedstuff of feed hygiene and nutritional need, make up the present situation that Xinjiang albumen feedstuff is not enough, obtained fermented product is yellow-brown solid, moistening and glossy, and easily clump together, crude protein, crude fibre, the conventional nutrients such as moisture are complete, crude protein content is 38.96%, and containing abundant mineral and trace element, heavy metal and pesticide remnant is in feedstuff allowed band, without miscellaneous bacterias such as Salmonellas, mould bacterial content is also in allowed band, meet the standard of mixed feed. the enzyme class that the method has filtered out degraded peento skin slag best is cellulase and pectase, and determine the technological parameter of complex enzyme hydrolysis, further define the process conditions being processed into microbial protein feed by peento skin slag simultaneously, with discarded peento skin slag for raw material, various lactobacillus is utilized to carry out fermenting experiment, turn waste into wealth, it is achieved the reprocessing of peento skin slag utilizes.
Accompanying drawing explanation
Fig. 1 is shown as the process certification procedure chart of a kind of method utilizing peento skin slag to produce albumen feedstuff provided by the invention.
Detailed description of the invention
Bacteria used thereby of the present invention is originated:
Candidiasis: purchased from Shandong sage fine jade biology company limited.
Aspergillus oryzae: purchased from Changtai, Shandong bio tech ltd.
Lactobacillus plantarum: Bai Fude bio tech ltd, Wuxi.
Embodiment 1:
A kind of method utilizing peento skin slag to produce albumen feedstuff, comprises the following steps:
1) peento skin slag is carried out complex enzyme hydrolysis, obtain peento skin slag after complex enzyme hydrolysis; Peento skin slag is the leftover bits and pieces produced in the flat peach wine course of processing, and compound enzyme used by complex enzyme hydrolysis is cellulase and pectase, and complex enzyme hydrolysis condition is: cellulase consumption 68mg/g, pectase consumption 20mg/g, and temperature is 40 DEG C, pH4.5, and the time is 4h; After complex enzyme hydrolysis, content of reducing sugar is 4.69%;
2) fermentation medium is prepared with peento skin slag after the complex enzyme hydrolysis that step 1) obtains for major ingredient, access combination bacterial strain carries out fermentation and prepares crude protein feed, consisting of of fermentation medium: peento skin slag 88 parts in parts by weight, sunflower seed dregs 9 parts, 1 part of wheat bran, 1 part of carbamide, 1 part of ammonium sulfate;In described fermentation medium, moisture is that 64%-66%, pH are natural; Described combination bacterial strain is candidiasis, aspergillus oryzae and Lactobacillus plantarum; Fermentation condition is: inoculum concentration is 10% altogether, and inoculative proportion is 1:1:1, and fermentation time is 3d, and fermentation temperature is 32 DEG C; After fermentation, crude protein content is 38.96%.
Embodiment 2:
A kind of method utilizing peento skin slag to produce albumen feedstuff provided by the invention, process certification process is as shown in Figure 1.
, complex enzyme hydrolysis condition determines:
(1) by peento skin slag nutritional labeling is carried out Preliminary detection, show that peento skin slag crude protein and content of reducing sugar are low, crude fibre, starch, pectin content high. For this feature, selecting cellulase, pectase and amylase that it is carried out enzymolysis, tentatively show that the hydrolysis result of cellulase and pectase is notable, amylase effect is not notable, is eliminated by amylase.
(2) in the enzymolysis of peento skin slag is studied, the hydrolysis result of compound enzyme is more notable than the hydrolysis result of single enzyme, and after adopting Single Fiber element enzyme enzymolysis, the content of reducing sugar is 3.39%, and after single pectinase enzymatic hydrolysis, content of reducing sugar is 1.34%; After complex enzyme hydrolysis, content of reducing sugar is 4.69%. Complex enzyme hydrolysis condition is: cellulase consumption 68mg/g, pectase consumption 20mg/g, and temperature is 40 DEG C, pH4.5, and the time is 4h. Adopting this parameter to test, the content of reducing sugar reaches 4.69%, illustrates that peento skin slag is after cellulase and pectinase enzymatic hydrolysis, and cellulose and pectin content are decreased obviously, and content of reducing sugar significantly improves, and illustrate that complex enzyme hydrolysis experiment effect is notable.
, fermented bacterium determines:
(1) by flat board dibbling experiment and single bacterium fermenting experiment, filter out from 9 kinds of strains and the strain of peento skin slag can be utilized to have candida mycoderma (Candidatails), aspergillus niger (AspergillusNiger), aspergillus oryzae (Aspergillusoryzae), Lactobacillus fermenti (Lactobacillusfermentum), Lactobacillus plantarum (Lactobacillusplantarum), koumiss sample lactobacillus (Lactobacilluskoumisssamples), Lactobacilluskefir, and utilizing the peento skin slag the highest single strain of white ability of laying eggs is candida mycoderma, crude protein content is 35.43%.
(2) in single bacterium fermentation, A bacterial strain (candidiasis) ferments albuminiferous ability higher than other single bacterial strain, and its crude protein content is 35.43%; In double; two bacterium mixed fermentations, AC combination (candida mycoderma+aspergillus oryzae) lays eggs white ability higher than other pair of bacterium combination, and its crude protein content is 35.98%; In three bacterium mixed fermentations, ACE combines (candida mycoderma+aspergillus oryzae+Lactobacillus plantarum) white ability of laying eggs higher than the mixing of other three bacterium, and its crude protein content is 36.63%.
(3) single bacterial strain and hybrid bacterial strain fermentation peento skin slag produce crude protein result and show: the three double; two bacterium bacterium combination mono-bacterium of > of bacterium combination >. The ability of three bacterium combination generation crude protein is significantly higher than single bacterium fermentation and produces the ability of crude protein. Thus illustrating, the best of breed of bacterial strain is ACE(candida mycoderma+aspergillus oryzae+Lactobacillus plantarum).
, fermentation medium and fermentation condition determine:
(1) first using aspergillus oryzae as zymocyte, investigated its Utilization ability to different auxiliary material and different nitrogen sources. Result of study shows, carbamide and ammonium sulfate are had good assimilative capacicy by this strain, and two kinds of nitrogenous sources can complement each other, so selecting carbamide and ammonium sulfate as nitrogenous source in experiment below.Sunflower seed dregs, wheat bran and cotton seed hulls are also had a good Utilization ability by aspergillus oryzae, but comprehensive protein content, palatability and cost consideration, select sunflower seed dregs as fermentation adjuvant. Finally determine with peento skin slag for fermentation major ingredient, sunflower seed skin, wheat bran and cotton seed hulls are experiment adjuvant, carbamide and ammonium sulfate are experiment nitrogenous source design culture medium, the fermentative medium formula filtering out optimum is: peento skin slag 88g, sunflower seed dregs 9g, wheat bran 1g, carbamide 1g, ammonium sulfate 1g, moisture is about 65%, and pH is natural.
(2) by the impact of crude protein content in tunning is studied by inoculum concentration, inoculative proportion, fermentation time, fermentation temperature and five single factor test of fermentation substrate injected volume in experiment, show that to three factors that product crude protein content is maximum be fermentation time, fermentation temperature and fermentation substrate injected volume, after three factors are carried out orthogonal experiment, the optimum process condition drawing fermentation is: inoculum concentration is 10%, inoculative proportion is 1:1:1, fermentation time is 3d, fermentation temperature is 32 DEG C, and fermentation substrate injected volume is 45g.
Fermented product is yellow-brown solid, moistening and glossy, and easily clump together, the conventional nutrients such as crude protein, crude fibre, moisture are complete, and containing abundant mineral and trace element, heavy metal and pesticide remnant is in feedstuff allowed band, without miscellaneous bacterias such as Salmonellas, mould bacterial content is also in allowed band, sum it up, this fermented product meets the standard of mixed feed.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention, although the present invention being described in detail with reference to previous embodiment, for a person skilled in the art, technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature carries out equivalent replacement. All within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (7)
1. one kind utilizes the method that peento skin slag produces albumen feedstuff, it is characterised in that comprise the following steps:
1) peento skin slag is carried out complex enzyme hydrolysis, obtain peento skin slag after complex enzyme hydrolysis;
2) prepare fermentation medium with peento skin slag after the complex enzyme hydrolysis that step 1) obtains for major ingredient, access combination bacterial strain and carry out fermentation and prepare crude protein feed.
2. a kind of method utilizing peento skin slag to produce albumen feedstuff according to claim 1, it is characterised in that in described step 1), peento skin slag is the leftover bits and pieces produced in the flat peach wine course of processing.
3. a kind of method utilizing peento skin slag to produce albumen feedstuff according to claim 2, it is characterised in that in described step 1), compound enzyme used by complex enzyme hydrolysis is cellulase and pectase.
4. a kind of method utilizing peento skin slag to produce albumen feedstuff according to claim 3, it is characterised in that in described step 1), complex enzyme hydrolysis condition is: cellulase consumption 68mg/g, pectase consumption 20mg/g, and temperature is 40 DEG C, pH4.5, the time is 4h; After complex enzyme hydrolysis, content of reducing sugar is 4.69%.
5. a kind of method utilizing peento skin slag to produce albumen feedstuff according to claim 4, it is characterised in that described step 2) in, consisting of of fermentation medium: peento skin slag 88 parts in parts by weight, sunflower seed dregs 9 parts, 1 part of wheat bran, 1 part of carbamide, 1 part of ammonium sulfate;In described fermentation medium, moisture is that 64%-66%, pH are natural.
6. a kind of method utilizing peento skin slag to produce albumen feedstuff according to claim 5, it is characterised in that described step 2) in, described combination bacterial strain is candidiasis, aspergillus oryzae and Lactobacillus plantarum.
7. a kind of method utilizing peento skin slag to produce albumen feedstuff according to claim 6, it is characterised in that described step 2) in, fermentation condition is: inoculum concentration is 10% altogether, and inoculative proportion is 1:1:1, and fermentation time is 3d, and fermentation temperature is 32 DEG C; After fermentation, crude protein content is 38.96%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108575940A (en) * | 2018-03-23 | 2018-09-28 | 哈尔滨伟平科技开发有限公司 | A kind of production method of fresh water fish bait |
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| CN102960538A (en) * | 2012-12-03 | 2013-03-13 | 广西壮族自治区分析测试研究中心 | Unicellular protein feed prepared from fermented dragon fruit peel and production method of unicellular protein feed |
| CN104286406A (en) * | 2014-11-10 | 2015-01-21 | 湖南鑫洋食品工业有限公司 | Orange peel feed production method |
| CN105053537A (en) * | 2015-08-10 | 2015-11-18 | 中国科学院重庆绿色智能技术研究院 | High protein feed producing method using orange peel residues as raw materials and feed |
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- 2016-01-05 CN CN201610001683.1A patent/CN105639117A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960538A (en) * | 2012-12-03 | 2013-03-13 | 广西壮族自治区分析测试研究中心 | Unicellular protein feed prepared from fermented dragon fruit peel and production method of unicellular protein feed |
| CN104286406A (en) * | 2014-11-10 | 2015-01-21 | 湖南鑫洋食品工业有限公司 | Orange peel feed production method |
| CN105053537A (en) * | 2015-08-10 | 2015-11-18 | 中国科学院重庆绿色智能技术研究院 | High protein feed producing method using orange peel residues as raw materials and feed |
Non-Patent Citations (1)
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| 杨丽: "蟠桃皮渣生产蛋白饲料技术研究", 《中国优秀硕士学位论文全文数据库（电子期刊）》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108575940A (en) * | 2018-03-23 | 2018-09-28 | 哈尔滨伟平科技开发有限公司 | A kind of production method of fresh water fish bait |
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