CN105638773B - 一种用于手术室器械消毒的多元纳米喷雾剂及其制备方法 - Google Patents
一种用于手术室器械消毒的多元纳米喷雾剂及其制备方法 Download PDFInfo
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- CN105638773B CN105638773B CN201610025253.3A CN201610025253A CN105638773B CN 105638773 B CN105638773 B CN 105638773B CN 201610025253 A CN201610025253 A CN 201610025253A CN 105638773 B CN105638773 B CN 105638773B
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Abstract
本发明公开了一种用于手术室器械消毒的多元纳米喷雾剂及其制备方法，所述用于手术室器械消毒的多元纳米喷雾剂是由以下成分制备而成：丙烯乙二醇、亚磷酸、纳米麦饭石粉、柠檬酸钠苯并三氮唑、氟伐他汀、硫酸锌、香根鸢尾提取物、荭草提取物、芦荟大黄素、茉莉酸、六棱菊提取物、2‑苯氧基乙醇、丙硫菌唑、4‑氯‑3,5‑二甲基苯酚、异菌脲、亚磷酸、肟菌酯、炔氨基甲酸叔丁酯、乳化剂、消泡剂、表面活性剂、液体稳定剂、乙醇、去离子水，本发明用于手术室器械消毒的多元纳米喷雾剂具有抗菌、润滑、防腐三重功效，可广泛应用与手术室器械的清洗消毒，有效杀灭各种细菌、病毒，无残留，预防医院交叉感染。
Description
技术领域
本发明涉及手术室器械消毒技术领域，具体是涉及一种用于手术室器械消毒的多元纳米喷雾剂及其制备方法。
背景技术
手术室器械使用后需要进行彻底的清洁处理，去除附着在上面的血液、粘液、体液等有机物，这是预防和控制医院感染，保证医疗安全的重要环节。根据《中国消毒学杂质》报道，美国每年有1500万例次由于内镜清洗消毒不当而引起的医院感染，主要是由绿脓杆菌、结核分歧杆菌和乙型肝炎病毒引起的感染。因此，将污染在手术器械上的有机物、无机物和微生物清除到安全的水平，保证灭菌的彻底性，控制医院交叉感染具有重要的作用。
清洗前采用一般的物理或化学消毒方法会导致手术器械上的蛋白质凝固变性，增加清洗难度，所以，手术室器械一般先用流水刷净血污，然后送入超声波清洗器，震荡清洗，然后风干，送入高压蒸汽消毒锅消毒，或者用0.1％新洁尔灭浸泡小消毒，或者用0.1洗必泰浸泡消毒，或者先煮沸1小时，后用0.2戊二醛，浸泡消毒。但是这些方法均不能完全彻底地杀灭细菌和病毒，同时对手术器械有一定的损伤，因此，需要研制出一种新型的消毒剂。
发明内容
本发明解决的技术问题是提供一种用于手术室器械消毒的多元纳米喷雾剂及其制备方法，具有抗菌、润滑、防腐三重功效。
本发明的技术方案是:一种用于手术室器械消毒的多元纳米喷雾剂，是由以下成分制备而成：丙烯乙二醇2.0-3.5％、亚磷酸2.0-3.0％、纳米麦饭石粉1.5-3.0％、柠檬酸钠1.2-2.0％、苯并三氮唑0.8-1.0％、氟伐他汀0.4-0.8％、硫酸锌0.5-0.9％、香根鸢尾提取物0.5-1.0％、荭草提取物0.5-0.8％、芦荟大黄素0.2-0.5％、茉莉酸0.1-0.2％、六棱菊提取物0.1-0.15％、2-苯氧基乙醇0.05-0.2％、丙硫菌唑0.05-0.15％、4-氯-3,5-二甲基苯酚0.03-0.1％、异菌脲0.02-0.1％、肟菌酯0.04-0.2％、炔氨基甲酸叔丁酯0.01-0.08％、乳化剂0.01-0.3％、消泡剂0.01-0.02％、表面活性剂0.05-0.1％、液体稳定剂0.02-0.04％、乙醇25-47％、余量为去离子水。
进一步地，在上述方案中，所述香根鸢尾提取物为香根鸢尾黄酮和香根鸢尾多酚按任意比组成的混合物，具有解毒杀菌的功效。
进一步地，在上述方案中，所述荭草提取物为荭草素，具有抗菌和抗病毒功效。
进一步地，在上述方案中，所述六棱菊提取物为二咖啡酰奎尼酸类化合物，能够抗乙型肝炎等病毒。
进一步地，在上述方案中，所述乳化剂为失水山梨醇脂肪酸酯中的任意一种，具有较好的乳化作用，形成均一稳定的溶液。
进一步地，在上述方案中，所述消泡剂为聚醚类消泡剂，包括聚氧乙烯聚氧丙烯季戊四醇醚、聚氧乙烯聚氧丙醇胺醚、聚氧丙烯甘油醚和聚氧丙烯聚氧乙烯甘油醚，降低液体表面张力，抑制泡沫产生。
进一步地，在上述方案中，所述表面活性剂为聚氧乙烯40氢化蓖麻油，具有润湿、抗粘、增溶、分散、洗涤及防腐等作用。
进一步地，在上述方案中，所述液体稳定剂为聚乙烯醇，能增加混合物的稳定性能，保持化学平衡,降低表面张力,防止光、热分解或氧化分解等。
一种用于手术室器械消毒的多元纳米喷雾剂的制备方法，步骤如下：
(1)按所述重量百分比取丙烯乙二醇、亚磷酸、纳米麦饭石粉、柠檬酸钠苯并三氮唑、氟伐他汀、硫酸锌、香根鸢尾提取物、荭草提取物、芦荟大黄素、茉莉酸、六棱菊提取物、2-苯氧基乙醇、丙硫菌唑、4-氯-3,5-二甲基苯酚、异菌脲、亚磷酸、肟菌酯、炔氨基甲酸叔丁酯、乳化剂、消泡剂、表面活性剂、液体稳定剂、乙醇、去离子水，待用；
(2)将乙醇和去离子水混合，依次加入丙烯乙二醇、亚磷酸、纳米麦饭石粉、柠檬酸钠苯并三氮唑、氟伐他汀、硫酸锌、香根鸢尾提取物、荭草提取物、芦荟大黄素、茉莉酸、六棱菊提取物、2-苯氧基乙醇、丙硫菌唑、4-氯-3,5-二甲基苯酚、异菌脲、亚磷酸、肟菌酯、炔氨基甲酸叔丁酯、乳化剂、表面活性剂，以240-300r/min的转速搅拌10-15min，用研磨机研磨30-60min，再加入消泡剂、液体稳定剂，继续以300-400r/min的转速搅拌3-8min，灭菌，用喷雾剂灌装机灌装，即成。
本发明的有益效果是：本发明用于手术室器械消毒的多元纳米喷雾剂具有抗菌、润滑、防腐三重功效，可广泛应用与手术室器械的清洗消毒，有效杀灭各种细菌、病毒，无残留，预防医院交叉感染，对手术器械无腐蚀，且能保护手术器械，延长其使用寿命，节约资源。另外，本发明制备工艺简单，成本低，适合大量生产。
具体实施方式
实施例1：
一种用于手术室器械消毒的多元纳米喷雾剂，是由以下成分制备而成：丙烯乙二醇2.0％、亚磷酸2.0％、纳米麦饭石粉1.5％、柠檬酸钠1.2％、苯并三氮唑0.8％、氟伐他汀0.4％、硫酸锌0.5％、香根鸢尾提取物0.5％、荭草提取物0.5％、芦荟大黄素0.2％、茉莉酸0.1％、六棱菊提取物0.1％、2-苯氧基乙醇0.05％、丙硫菌唑0.05％、4-氯-3,5-二甲基苯酚0.03％、异菌脲0.02％、肟菌酯0.04％、炔氨基甲酸叔丁酯0.01％、乳化剂0.01％、消泡剂0.01％、表面活性剂0.05％、液体稳定剂0.02％、乙醇25％、余量为去离子水。
其中，所述香根鸢尾提取物为香根鸢尾黄酮和香根鸢尾多酚按任意比组成的混合物，具有解毒杀菌的功效。所述荭草提取物为荭草素，具有抗菌和抗病毒功效。所述六棱菊提取物为二咖啡酰奎尼酸类化合物，能够抗乙型肝炎等病毒。所述乳化剂为失水山梨醇脂肪酸酯，具有较好的乳化作用，形成均一稳定的溶液。所述消泡剂为聚氧乙烯聚氧丙烯季戊四醇醚，降低液体表面张力，抑制泡沫产生。所述表面活性剂为聚氧乙烯40氢化蓖麻油，具有润湿、抗粘、增溶、分散、洗涤及防腐等作用。所述液体稳定剂为聚乙烯醇，能增加混合物的稳定性能，保持化学平衡,降低表面张力,防止光、热分解或氧化分解等。
一种用于手术室器械消毒的多元纳米喷雾剂的制备方法，步骤如下：
(1)按所述重量百分比取丙烯乙二醇、亚磷酸、纳米麦饭石粉、柠檬酸钠苯并三氮唑、氟伐他汀、硫酸锌、香根鸢尾提取物、荭草提取物、芦荟大黄素、茉莉酸、六棱菊提取物、2-苯氧基乙醇、丙硫菌唑、4-氯-3,5-二甲基苯酚、异菌脲、亚磷酸、肟菌酯、炔氨基甲酸叔丁酯、乳化剂、消泡剂、表面活性剂、液体稳定剂、乙醇、去离子水，待用；
(2)将乙醇和去离子水混合，依次加入丙烯乙二醇、亚磷酸、纳米麦饭石粉、柠檬酸钠苯并三氮唑、氟伐他汀、硫酸锌、香根鸢尾提取物、荭草提取物、芦荟大黄素、茉莉酸、六棱菊提取物、2-苯氧基乙醇、丙硫菌唑、4-氯-3,5-二甲基苯酚、异菌脲、亚磷酸、肟菌酯、炔氨基甲酸叔丁酯、乳化剂、表面活性剂，以240r/min的转速搅拌10min，用研磨机研磨30min，再加入消泡剂、液体稳定剂，继续以300r/min的转速搅拌3min，灭菌，用喷雾剂灌装机灌装，即成。
实施例2：
一种用于手术室器械消毒的多元纳米喷雾剂，是由以下成分制备而成：丙烯乙二醇2.75％、亚磷酸2.5％、纳米麦饭石粉2.25％、柠檬酸钠1.6％、苯并三氮唑0.9％、氟伐他汀0.6％、硫酸锌0.7％、香根鸢尾提取物0.75％、荭草提取物0.65％、芦荟大黄素0.35％、茉莉酸0.15％、六棱菊提取物0.125％、2-苯氧基乙醇0.125％、丙硫菌唑0.1％、4-氯-3,5-二甲基苯酚0.65％、异菌脲0.06％、肟菌酯0.12％、炔氨基甲酸叔丁酯0.045％、乳化剂0.155％、消泡剂0.015％、表面活性剂0.075％、液体稳定剂0.03％、乙醇36％、余量为去离子水。
其中，所述香根鸢尾提取物为香根鸢尾黄酮和香根鸢尾多酚按任意比组成的混合物，具有解毒杀菌的功效。所述荭草提取物为荭草素，具有抗菌和抗病毒功效。所述六棱菊提取物为二咖啡酰奎尼酸类化合物，能够抗乙型肝炎等病毒。所述乳化剂为失水山梨醇脂肪酸酯，具有较好的乳化作用，形成均一稳定的溶液。所述消泡剂为聚氧乙烯聚氧丙醇胺醚，降低液体表面张力，抑制泡沫产生。所述表面活性剂为聚氧乙烯40氢化蓖麻油，具有润湿、抗粘、增溶、分散、洗涤及防腐等作用。所述液体稳定剂为聚乙烯醇，能增加混合物的稳定性能，保持化学平衡,降低表面张力,防止光、热分解或氧化分解等。
一种用于手术室器械消毒的多元纳米喷雾剂的制备方法，步骤如下：
(1)按所述重量百分比取丙烯乙二醇、亚磷酸、纳米麦饭石粉、柠檬酸钠苯并三氮唑、氟伐他汀、硫酸锌、香根鸢尾提取物、荭草提取物、芦荟大黄素、茉莉酸、六棱菊提取物、2-苯氧基乙醇、丙硫菌唑、4-氯-3,5-二甲基苯酚、异菌脲、亚磷酸、肟菌酯、炔氨基甲酸叔丁酯、乳化剂、消泡剂、表面活性剂、液体稳定剂、乙醇、去离子水，待用；
(2)将乙醇和去离子水混合，依次加入丙烯乙二醇、亚磷酸、纳米麦饭石粉、柠檬酸钠苯并三氮唑、氟伐他汀、硫酸锌、香根鸢尾提取物、荭草提取物、芦荟大黄素、茉莉酸、六棱菊提取物、2-苯氧基乙醇、丙硫菌唑、4-氯-3,5-二甲基苯酚、异菌脲、亚磷酸、肟菌酯、炔氨基甲酸叔丁酯、乳化剂、表面活性剂，以270r/min的转速搅拌12.5min，用研磨机研磨45min，再加入消泡剂、液体稳定剂，继续以350r/min的转速搅拌5.5min，灭菌，用喷雾剂灌装机灌装，即成。
实施例3：
一种用于手术室器械消毒的多元纳米喷雾剂，是由以下成分制备而成：丙烯乙二醇3.5％、亚磷酸3.0％、纳米麦饭石粉3.0％、柠檬酸钠2.0％、苯并三氮唑1.0％、氟伐他汀0.8％、硫酸锌0.9％、香根鸢尾提取物1.0％、荭草提取物0.8％、芦荟大黄素0.5％、茉莉酸0.2％、六棱菊提取物0.15％、2-苯氧基乙醇0.2％、丙硫菌唑0.15％、4-氯-3,5-二甲基苯酚0.1％、异菌脲0.1％、肟菌酯0.2％、炔氨基甲酸叔丁酯0.08％、乳化剂0.3％、消泡剂.02％、表面活性剂0.1％、液体稳定剂0.04％、乙醇47％、余量为去离子水。
其中，所述香根鸢尾提取物为香根鸢尾黄酮和香根鸢尾多酚按任意比组成的混合物，具有解毒杀菌的功效。所述荭草提取物为荭草素，具有抗菌和抗病毒功效。所述六棱菊提取物为二咖啡酰奎尼酸类化合物，能够抗乙型肝炎等病毒。所述乳化剂为失水山梨醇脂肪酸酯，具有较好的乳化作用，形成均一稳定的溶液。所述消泡剂为聚氧丙烯甘油醚，降低液体表面张力，抑制泡沫产生。所述表面活性剂为聚氧乙烯40氢化蓖麻油，具有润湿、抗粘、增溶、分散、洗涤及防腐等作用。所述液体稳定剂为聚乙烯醇，能增加混合物的稳定性能，保持化学平衡,降低表面张力,防止光、热分解或氧化分解等。
一种用于手术室器械消毒的多元纳米喷雾剂的制备方法，步骤如下：
(1)按所述重量百分比取丙烯乙二醇、亚磷酸、纳米麦饭石粉、柠檬酸钠苯并三氮唑、氟伐他汀、硫酸锌、香根鸢尾提取物、荭草提取物、芦荟大黄素、茉莉酸、六棱菊提取物、2-苯氧基乙醇、丙硫菌唑、4-氯-3,5-二甲基苯酚、异菌脲、亚磷酸、肟菌酯、炔氨基甲酸叔丁酯、乳化剂、消泡剂、表面活性剂、液体稳定剂、乙醇、去离子水，待用；
(2)将乙醇和去离子水混合，依次加入丙烯乙二醇、亚磷酸、纳米麦饭石粉、柠檬酸钠苯并三氮唑、氟伐他汀、硫酸锌、香根鸢尾提取物、荭草提取物、芦荟大黄素、茉莉酸、六棱菊提取物、2-苯氧基乙醇、丙硫菌唑、4-氯-3,5-二甲基苯酚、异菌脲、亚磷酸、肟菌酯、炔氨基甲酸叔丁酯、乳化剂、表面活性剂，以300r/min的转速搅拌15min，用研磨机研磨60min，再加入消泡剂、液体稳定剂，继续以400r/min的转速搅拌8min，灭菌，用喷雾剂灌装机灌装，即成。
一、灭菌实验：
1、实验材料
1.1、试验菌株为金黄色葡萄球菌、绿脓杆菌、肺炎链球菌、大肠杆菌、乙型肝炎病毒株、HIV病毒株。
1.2、实验样品：D1、D2、D3、D4样品，4℃存放备用。其中D1至D3依次对应实施例3、2、1和洗必泰溶液。
1.3、培养基：金黄色葡萄球菌培养基、绿脓杆菌培养基、肺炎链球菌培养基、大肠杆菌培养基、乙型肝炎病毒株培养基、HIV病毒株培养基，调节pH为6.6～7.0。其中固体培养基中加入琼脂15g/L。
2、实验方法
2.1、菌悬液制备及接种
分别取冷冻保藏菌悬液0.1ml滴加于5ml金黄色葡萄球菌培养基、绿脓杆菌培养基、肺炎链球菌培养基、大肠杆菌培养基、乙型肝炎病毒株培养基、HIV病毒株培养基中，于37℃厌氧条件培养2d，为实验菌悬液。
2.2、药液制备：将D1、D2、D3、D4分别用生理盐水稀释成试验浓度0.1％，然后分别梯度稀释。第一次试验梯度稀释浓度分别为100％(样品原液)、50％、25％、12.5％、6.25％、3.125％、1.56％、0.78％。第二次试验复测梯度稀释浓度分别为10％、8％、6％、4％、2％。
2.3、菌液制备
分别用金黄色葡萄球菌、绿脓杆菌、肺炎链球菌、大肠杆菌、乙型肝炎病毒株、HIV病毒株的液体培养基稀释菌悬液，使其最终菌浓度为l06CFU/ml。
2.4、培养及结果判读：在微孔中加入菌液100μL和药液100μL，同时设不加菌的阴性对照和不加药液的正常生长对照，每种药做3个平行，取平均值。置37℃厌氧孵育，48h后观察结果。采用肉眼判断有无浑浊，采用直接法读取数据。结果判断的前提是生长对照良好，空白对照无菌生长清晰，其它孔随药物浓度梯度升高而菌的生长受到抑制。
表1：金黄色葡萄球菌抑菌梯度试验结果
| 各组浓度梯度 | 50％ | 25％ | 12.5％ | 6.25％ | 3.125％ | 1.56％ | 0.78％ |
| D1 | - | - | - | - | - | + | + |
| D2 | - | - | - | - | - | + | + |
| D3 | - | - | - | - | + | + | + |
| D4 | - | - | + | + | + | + | + |
注：其中+代表有菌生长，-代表无。
表2绿脓杆菌抑菌梯度试验结果
| 各组浓度梯度 | 50％ | 25％ | 12.5％ | 6.25％ | 3.125％ | 1.56％ | 0.78％ |
| D1 | - | - | - | - | - | - | + |
| D2 | - | - | - | - | - | + | + |
| D3 | - | - | - | - | + | + | + |
| D4 | - | - | + | + | + | + | + |
注：其中+代表有菌生长，-代表无。
表3肺炎链球菌抑菌梯度试验结果
| 各组浓度梯度 | 50％ | 25％ | 12.5％ | 6.25％ | 3.125％ | 1.56％ | 0.78％ |
| D1 | - | - | - | - | - | - | + |
| D2 | - | - | - | - | - | - | + |
| D3 | - | - | - | - | - | + | + |
| D4 | - | - | + | + | + | + | + |
注：其中+代表有菌生长，-代表无。
表4大肠杆菌抑菌梯度试验结果
| 各组浓度梯度 | 50％ | 25％ | 12.5％ | 6.25％ | 3.125％ | 1.56％ | 0.78％ |
| D1 | - | - | - | - | - | - | - |
| D2 | - | - | - | - | - | + | + |
| D3 | - | - | - | - | + | + | + |
| D4 | - | - | + | + | + | + | + |
注：其中+代表有菌生长，-代表无。
2.5、结果：从表1至表4的数据可见，3组实施例0.1％的溶液，均在其稀释至12.5％浓度及以上，对金黄色葡萄球菌、绿脓杆菌、肺炎链球菌、大肠杆菌均有良好抑制作用，而对照例中D4在25％稀释液及以上才能达到效果，从上述结果可见，经过验证，D1、D2的抑菌浓度可在3.125％稀释液达到，而D3也在6.25％的浓度达到了抑菌效果。
二、抗病毒实验：
1、实验材料
1.1、MDCK细胞、病毒株、试剂：MDCK细胞、乙型肝炎病毒株、HIV病毒株，均购自美国模式菌种保藏中心(ATCC)；试剂：D2，实施例2制备的多元纳米喷雾剂。
1.2、实验仪器：多标记微孔板读取仪(Perkin Elmer)。
2、实验方法与结果
2.1D2对乙型肝炎病毒株和HIV病毒株的抗病毒活性评价：
本实验分两组同时进行：
第一组：将MDCK细胞按1.5×104细胞/孔接种于96孔细胞培养板中，37℃细胞培养箱中培养14～18h后，待细胞长成单层后备用。将孔板中培养基弃去，PBS液洗两遍，加100TCID50/孔(100μl)乙型肝炎病毒感染细胞，同时加入各梯度浓度的药物(以100.0μM为起始浓度，连续2倍梯度稀释6个梯度，每梯度两个复孔)至总体积为200μl的细胞维持培养液(DMEM+0.3％BSA+2.5ug/L胰酶)中，于细胞培养箱中37℃培养72h后，用LuciferaseAssay System试剂盒检测荧光素酶活性。
第二组：将MDCK细胞按1.5×104细胞/孔接种于96孔细胞培养板中，37℃细胞培养箱中培养14～18h后，待细胞长成单层后备用。将孔板中培养基弃去，PBS液洗两遍，加100TCID50/孔(100μl)HIV病毒感染细胞，同时加入各梯度浓度的药物(以100.0μM为起始浓度，连续2倍梯度稀释6个梯度，每梯度两个复孔)至总体积为200μl的细胞维持培养液(DMEM+0.3％BSA+2.5ug/L胰酶)中，于细胞培养箱中37℃培养72h后，用Luciferase AssaySystem试剂盒检测荧光素酶活性。
结果发现，D2能够有效抑制乙型肝炎病毒株和HIV病毒株的活性，并抑制其复制。
以上述依据本发明的理想实施例为启示，通过上述的说明内容，相关工作人员完全可以在不偏离本项发明技术思想的范围内，进行多样的变更以及修改。本项发明的技术性范围并不局限于说明书上的内容，必须要根据权利要求范围来确定其技术性范围。
Claims (1)
1.一种用于手术室器械消毒的多元纳米喷雾剂，其特征在于，是由以下成分制备而成：丙烯乙二醇2.0-3.5％、亚磷酸2.0-3.0％、纳米麦饭石粉1.5-3.0％、柠檬酸钠1.2-2.0％、苯并三氮唑0.8-1.0％、氟伐他汀0.4-0.8％、硫酸锌0.5-0.9％、香根鸢尾提取物0.5-1.0％、荭草提取物0.5-0.8％、芦荟大黄素0.2-0.5％、茉莉酸0.1-0.2％、六棱菊提取物0.1-0.15％、2-苯氧基乙醇0.05-0.2％、丙硫菌唑0.05-0.15％、4-氯-3,5-二甲基苯酚0.03-0.1％、异菌脲0.02-0.1％、肟菌酯0.04-0.2％、炔氨基甲酸叔丁酯0.01-0.08％、乳化剂0.01-0.3％、消泡剂0.01-0.02％、表面活性剂0.05-0.1％、液体稳定剂0.02-0.04％、乙醇25-47％、余量为去离子水；
所述香根鸢尾提取物为香根鸢尾黄酮、香根鸢尾多酚；
所述荭草提取物为荭草素；
所述六棱菊提取物为二咖啡酰奎尼酸类化合物；
所述乳化剂为失水山梨醇脂肪酸酯中的任意一种；
所述消泡剂为聚醚类消泡剂，包括聚氧乙烯聚氧丙烯季戊四醇醚、聚氧乙烯聚氧丙醇胺醚、聚氧丙烯甘油醚和聚氧丙烯聚氧乙烯甘油醚；
所述表面活性剂为聚氧乙烯40氢化蓖麻油；
所述液体稳定剂为聚乙烯醇；
所述用于手术室器械消毒的多元纳米喷雾剂的制备方法，步骤如下：
(1)按所述重量百分比取丙烯乙二醇、亚磷酸、纳米麦饭石粉、柠檬酸钠苯并三氮唑、氟伐他汀、硫酸锌、香根鸢尾提取物、荭草提取物、芦荟大黄素、茉莉酸、六棱菊提取物、2-苯氧基乙醇、丙硫菌唑、4-氯-3,5-二甲基苯酚、异菌脲、亚磷酸、肟菌酯、炔氨基甲酸叔丁酯、乳化剂、消泡剂、表面活性剂、液体稳定剂、乙醇、去离子水，待用；
(2)将乙醇和去离子水混合，依次加入丙烯乙二醇、亚磷酸、纳米麦饭石粉、柠檬酸钠苯并三氮唑、氟伐他汀、硫酸锌、香根鸢尾提取物、荭草提取物、芦荟大黄素、茉莉酸、六棱菊提取物、2-苯氧基乙醇、丙硫菌唑、4-氯-3,5-二甲基苯酚、异菌脲、亚磷酸、肟菌酯、炔氨基甲酸叔丁酯、乳化剂、表面活性剂，以240-300r/min的转速搅拌10-15min，用研磨机研磨30-60min，再加入消泡剂、液体稳定剂，继续以300-400r/min的转速搅拌3-8min，灭菌，用喷雾剂灌装机灌装，即成。
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