CN105638529B - A method of differentiating black porgy red porgy and its hybrid generation based on morphological parameters - Google Patents
A method of differentiating black porgy red porgy and its hybrid generation based on morphological parameters Download PDFInfo
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- CN105638529B CN105638529B CN201610081300.6A CN201610081300A CN105638529B CN 105638529 B CN105638529 B CN 105638529B CN 201610081300 A CN201610081300 A CN 201610081300A CN 105638529 B CN105638529 B CN 105638529B
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Abstract
The present invention relates to a kind of methods differentiating black porgy red porgy and its hybrid generation based on morphological parameters, include the following steps：The morphological parameters of porgy class sample are measured, the morphological parameters include：Overall length, body are long, body is thick, eye diameter and eye back are long；Establish black porgy selfing group, red porgy selfing group, female red porgy and male black porgy hybridization group, female black porgy and male red porgy hybridization group discriminant equation；Obtained morphological parameters are brought into discriminant equation group so that it is determined that fingerling.The present invention can quickly, accurately differentiate black porgy, red porgy and its hybrid generation.
Description
Technical field
The present invention relates to fish authentication technique field, more particularly to one kind based on morphological parameters differentiate black porgy red porgy and its
The method of hybrid generation.
Background technology
Black porgy (Acanthopagrus schlegelii) is also known as black spine porgy, belongs to Perciformes (Perciformes), porgy section
(Sparidae), spine porgy category (Acanthopagrus).China coast produces it, more with Huanghai-Bohai seas yield.With temperature, salt
The features such as optimum range is wide, meat is fine and smooth, full of nutrition, premunition is strong is spent, but the speed of growth is slow, the culture-cycle is long, body colour
It is dark to sell difference.Red porgy (pagrosomus major) belongs to Perciformes (Perciformes), porgy section (Sparidae), red porgy
Belong to (Pagrosomus), the gorgeous face value of body colour is high, and the speed of growth is fast, but suitable temperature range is narrow, meat is coarse, resistance is poor.
Genetic cross technology is one of the main method of fish species selection and breeding and Genetic improvement.The basic principle of crossbreeding
It is that hybrid generation can be made to integrate the merit of parents by hybridization means, increase variability, can also generates available
Hybrid vigour；Its essence is being recombinated using the gene and character of existing Germplasm resources, different groups will be scattered in
The assortment of genes together, establishes desirable genotype and phenotype.
Based on hybrid vigour theory, the characteristics of respectively mutual supplement with each other's advantages in view of red porgy, black porgy, Jiangsu Prov. Inst. of Marine Aquatic Products
The crossbreeding for having carried out red porgy and black porgy in 2014 is studied, and successfully obtains reciprocal cross offspring, by the cultivation at 20 monthly ages,
Hybrid generation has been developed for adult fish, though fish body formalness has larger difference with red porgy, and it is very close with black porgy individual morphology.
For this purpose, searching is a kind of can to differentiate the problem of method of black porgy red porgy and its hybrid generation becomes urgent need to resolve.
Invention content
Technical problem to be solved by the invention is to provide one kind differentiating black porgy red porgy and its hybridization based on morphological parameters
The method in generation can quickly, accurately differentiate black porgy, red porgy and its hybrid generation.
The technical solution adopted by the present invention to solve the technical problems is：It provides a kind of true based on morphological parameters discriminating black porgy
The method of porgy and its hybrid generation, includes the following steps：
(1) morphological parameters of porgy class sample are measured, the morphological parameters include：After overall length, body length, body thickness, eye diameter and eye
It is long；
(2) black porgy selfing group, red porgy selfing group, female red porgy and male black porgy hybridization group, female black porgy and male are established
The discriminant equation of red porgy hybridization group；
(3) obtained morphological parameters are brought into discriminant equation group so that it is determined that fingerling.
Overall length in the step (1) in morphological parameters is that the fish body of porgy class sample kisses end to the length of tail fin end, is surveyed
The cephalocaudal axis keeping parallelism of porgy class sample when amount.
Body length in the step (1) in morphological parameters is fish body kiss end to last piece of vertebrae end of porgy class sample
Distance, the cephalocaudal axis keeping parallelism of porgy class sample when measurement.
Body thickness in the step (1) in morphological parameters is the fish body both sides maximum distance of porgy class sample, porgy class when measurement
The dorsoventral axis keeping parallelism of sample.
Eye diameter in the step (1) in morphological parameters is the eye socket internal diameter of the fish body of porgy class sample.
In the step (1) in morphological parameters eye back length be porgy class sample the eye socket rear to nucleus of fish body after
The distance of edge, the dorsoventral axis keeping parallelism of porgy class sample when measurement.
The discriminant equation is as follows：
Red porgy selfing group：Y1=3882.753X1+4285.806X2-1111.759X3+2752.870X4-1957.971；
Black porgy selfing group：Y2=3944.204X1+4204.179X2-1421.639X3+3211.476X4-2032.330；
Female red porgy and male black porgy hybridization group：Y3=4093.490X1+4220.458X2-1906.889X3+
2837.128X4-2084.382；
Female black porgy and male red porgy hybridization group：Y4=4097.927X1+4544.774X2-2096.034X3+
3289.582X4-2169.883；
Wherein, X1 is the ratio of body length and overall length, and X2 is the ratio of body thickness and overall length, and X3 is the ratio of eye diameter and overall length,
X4 is the ratio of eye back length and overall length.
In the step (3) fingerling of porgy class sample be substitute into discriminant equation in obtain numerical value closest to 0 it is corresponding
Fingerling.
Advantageous effect
Due to the adoption of the above technical solution, compared with prior art, the present invention having the following advantages that and actively imitating
Fruit：Red porgy, black porgy and its hybrid generation are rapidly and accurately distinguished by the present invention simultaneously with formalness parameter, are differentiated accurate
True rate reaches 99.17%, has the characteristics that easy to operate, quick, pin-point accuracy, can use manpower and material resources sparingly, to porgy class germplasm
Resource conservation plays the role of very important with utilization.
Description of the drawings
Fig. 1 is black porgy red porgy and its each measurement parameter schematic diagram of hybrid generation.
Specific implementation mode
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, people in the art
Member can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Range.
Embodiments of the present invention are related to a kind of method differentiating black porgy, red porgy and its hybrid generation based on morphological parameters,
Include the following steps：
1, acquire fresh black porgy selfing group, red porgy selfing group, female red porgy hybridize with male black porgy group, female black porgy and
Male red porgy hybridization group sample, every group of 30 tails individual；
2, with vernier caliper (precision 0.01cm) following parameter is measured with reference to Figure of description：
(1) overall length：Kiss (1) as shown in the figure at a distance from end to tail fin end (parallel with cephalocaudal axis)；
(2) body is long：Fish body is kissed at a distance from end to last piece of vertebrae end (parallel with cephalocaudal axis), is specifically being measured
In, to kiss (2) as shown in the figure at a distance from end to the last one scale end (parallel with cephalocaudal axis) of lateral line scales (or file squama)；
(3) body is thick：Fish body both sides (parallel with dorsoventral axis) maximum distance (3) as shown in the figure；
(4) eye diameter：Eye socket internal diameter (as shown in the figure 4) parallel with cephalocaudal axis；
(5) eye back is long：(5) as shown in the figure at a distance from eye socket rear to nucleus rear (parallel with cephalocaudal axis)；
3, X1 (body length/overall length), X2 (body thickness/overall length), X3 (eye diameter/overall length) and X4 (eye back length/complete are calculated separately
It is long)；
4, black porgy selfing group, red porgy selfing group, red porgy (♀) × black porgy (♂) hybridization group, black porgy (♀) × red porgy (♂) are established
Hybridization group discriminant equation is as follows：
Red porgy selfing group：Y1=3882.753X1+4285.806X2-1111.759X3+2752.870X4-1957.971；
Black porgy selfing group：Y2=3944.204X1+4204.179X2-1421.639X3+3211.476X4-2032.330；
Female red porgy hybridizes group with male black porgy：Y3=4093.490X1+4220.458X2-1906.889X3+
2837.128X4-2084.382；
Female black porgy hybridizes group with male red porgy：Y4=4097.927X1+4544.774X2-2096.034X3+
3289.582X4-2169.883。
5, the porgy class sample morphology supplemental characteristic that differentiates will be needed to substitute into above-mentioned discriminant equation, gained Y value is closest to 0
Person, as this group of fingerling.
Final result finds that 30 tail individual of red porgy selfing group is referred in red porgy selfing group, differentiates accurate as shown in table 1
True rate is 100%, and the differentiation accuracy rate that black porgy selfing group, female red porgy hybridize group with male black porgy is also 100%, only 1 tail
Female black porgy is mistaken for female red porgy and male black porgy hybrid generation with male red porgy hybrid generation, differentiates that accuracy rate is
96.67%, comprehensive distinguishing rate is 99.17%, it is shown that high accuracy.Illustrate that this method by gradually discriminatory analysis, reaches
The purpose for differentiating porgy class filial generation is arrived.
4 combination distinguishing results of 1 red porgy of table and black porgy selfing group and reciprocal cross group
Claims (7)
1. a kind of method differentiating black porgy red porgy and its hybrid generation based on morphological parameters, which is characterized in that include the following steps：
(1) morphological parameters of porgy class sample are measured, the morphological parameters include：Overall length, body are long, body is thick, eye diameter and eye back are long；
(2) black porgy selfing group, red porgy selfing group, female red porgy and male black porgy hybridization group, female black porgy and male red porgy are established
The discriminant equation of hybridization group；The discriminant equation is as follows：
Red porgy selfing group：Y1=3882.753X1+4285.806X2-1111.759X3+2752.870X4-1957.971；
Black porgy selfing group：Y2=3944.204X1+4204.179X2-1421.639X3+3211.476X4-2032.330；
Female red porgy and male black porgy hybridization group：Y3=4093.490X1+4220.458X2-1906.889X3+2837.128X4-
2084.382；
Female black porgy and male red porgy hybridization group：Y4=4097.927X1+4544.774X2-2096.034X3+3289.582X4-
2169.883；
Wherein, X1 is the ratio of body length and overall length, and X2 is the ratio of body thickness and overall length, and X3 is the ratio of eye diameter and overall length, and X4 is
The ratio of eye back length and overall length
(3) obtained morphological parameters are brought into discriminant equation group so that it is determined that fingerling.
2. the method according to claim 1 for being differentiated black porgy red porgy and its hybrid generation based on morphological parameters, feature are existed
End is kissed in the fish body that, the overall length in the step (1) in morphological parameters is porgy class sample to the length of tail fin end, porgy when measurement
The cephalocaudal axis keeping parallelism of class sample.
3. the method according to claim 1 for being differentiated black porgy red porgy and its hybrid generation based on morphological parameters, feature are existed
In the fish body that, the body length in the step (1) in morphological parameters is porgy class sample kiss end to last piece of vertebrae end away from
The cephalocaudal axis keeping parallelism of porgy class sample when from, measurement.
4. the method according to claim 1 for being differentiated black porgy red porgy and its hybrid generation based on morphological parameters, feature are existed
In, the body thickness in the step (1) in morphological parameters be the fish body both sides maximum distance of porgy class sample, porgy class sample when measurement
Dorsoventral axis keeping parallelism.
5. the method according to claim 1 for being differentiated black porgy red porgy and its hybrid generation based on morphological parameters, feature are existed
In the eye diameter in the step (1) in morphological parameters is the eye socket internal diameter of the fish body of porgy class sample.
6. the method according to claim 1 for being differentiated black porgy red porgy and its hybrid generation based on morphological parameters, feature are existed
In the eye back length in the step (1) in morphological parameters is the eye socket rear of the fish body of porgy class sample to nucleus rear
Distance, the dorsoventral axis keeping parallelism of porgy class sample when measurement.
7. the method according to claim 1 for being differentiated black porgy red porgy and its hybrid generation based on morphological parameters, feature are existed
In the fingerling of porgy class sample is to substitute into discriminant equation to obtain numerical value closest to 0 corresponding fingerling in the step (3).
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| CN106119377B (en) * | 2016-07-06 | 2019-11-29 | 中国水产科学研究院淡水渔业研究中心 | A kind of primer, kit and discrimination method identifying red porgy, black porgy and its filial generation |
| CN106119389B (en) * | 2016-08-23 | 2019-07-05 | 江苏省海洋水产研究所 | A method of identifying black porgy and black porgy red porgy reciprocal cross filial generation |
| CN109078861B (en) * | 2017-06-14 | 2020-11-27 | 北京市水产科学研究所 | Method for sorting body types of young yellow-edge cuora |
| CN109258541A (en) * | 2018-11-12 | 2019-01-25 | 南京师范大学 | A method of based on ratio Characters Identification Fugu category fish sex |
| CN112741028B (en) * | 2021-01-12 | 2021-11-02 | 湖南师范大学 | Method for rapidly identifying hybrid of Megalobrama amblycephala and crucian carp and evaluating megalobrama amblycephala gynogenesis efficiency induced by red crucian carp sperm |
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