CN105638529A - Method for discriminating acanthopagrus schlegelii, pagrosomus major and hybrid offsprings thereof based on morphological parameters - Google Patents
Method for discriminating acanthopagrus schlegelii, pagrosomus major and hybrid offsprings thereof based on morphological parameters Download PDFInfo
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- CN105638529A CN105638529A CN201610081300.6A CN201610081300A CN105638529A CN 105638529 A CN105638529 A CN 105638529A CN 201610081300 A CN201610081300 A CN 201610081300A CN 105638529 A CN105638529 A CN 105638529A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention relates to a method for discriminating acanthopagrus schlegelii, pagrosomus major and hybrid offsprings thereof based on morphological parameters. The method includes the following steps that the morphological parameters of a sparidae sample are measured, wherein the morphological parameters comprise total length, body length, body thickness, eye diameter and eye posterior segment length; discriminant equations for an acanthopagrus schlegelii selfing group, a pagrosomus major selfing group, a female pagrosomus major and male acanthopagrus schlegelii hybridization group and a female acanthopagrus schlegelii and male pagrosomus major hybridization group are established; the obtained morphological parameters are substituted into the discriminant equation set to determine fish varieties. By means of the method, acanthopagrus schlegelii, pagrosomus major and hybrid offsprings thereof can be rapidly and accurately discriminated.
Description
Technical field
The present invention relates to Fish authentication technique field, particularly relate to a kind of method differentiating black porgy snapper and hybrid generation thereof based on morphological parameters.
Background technology
Black porgy (Acanthopagrusschlegelii), also known as black spine Channa argus, belongs to Perciformes (Perciformes), Channa argus section (Sparidae), and spine Channa argus belongs to (Acanthopagrus). China coast all produces it, more with Huanghai-Bohai seas yield. The feature such as there is temperature, salinity optimum range is wide, meat is fine and smooth, nutritious, premunition is strong, but the speed of growth slowly, culture-cycle length, body colour are dark sells difference. Snapper (pagrosomusmajor) belongs to Perciformes (Perciformes), Channa argus section (Sparidae), snapper belongs to (Pagrosomus), and the gorgeous face value of body colour is high, fast growth, but suitable temperature range is narrow, meat is coarse, resistance is poor.
Genetic cross technology is one of fish species selection-breeding main method with Genetic improvement. The ultimate principle of cross-breeding is can be made the merit of the comprehensive parents of hybrid generation by hybridization means, be increased variability, it is also possible to produce available hybrid vigor; Its essence is and utilize the gene of existing Germplasm resources and character to recombinate, by being scattered in the assortment of genes of different groups together, set up desirable genotype and phenotype.
Theoretical based on hybrid vigor, in view of the feature that snapper, black porgy are each had complementary advantages, Jiangsu Prov. Inst. of Marine Aquatic Products carried out the cross-breeding of snapper and black porgy and has studied in 2014, and it is successfully obtained reciprocal crosses offspring, cultivation through 20 monthly ages, hybrid generation has been grown for adult fish, though fish external body form and snapper have a larger difference, but with black porgy individual morphology closely. For this, finding a kind of method that can differentiate black porgy snapper and hybrid generation thereof becomes the problem needing solution badly.
Summary of the invention
The technical problem to be solved is to provide a kind of method differentiating black porgy snapper and hybrid generation thereof based on morphological parameters, it is possible to quickly, accurately differentiate black porgy, snapper and hybrid generation thereof.
The technical solution adopted for the present invention to solve the technical problems is: provides a kind of method differentiating black porgy snapper and hybrid generation thereof based on morphological parameters, comprises the following steps:
(1) measuring the morphological parameters of Channa argus class sample, described morphological parameters includes: total length, body length, body thickness, eye footpath and eye back are long;
(2) discriminant equation of black porgy selfing group, snapper selfing group, female snapper and male black porgy hybridization group, female black porgy and male snapper hybridization group is set up;
(3) morphological parameters obtained is brought in discriminant equation group so that it is determined that fingerling.
In described step (1), the total length in morphological parameters is the fish body kiss end length to tail fin end of Channa argus class sample, the cephalocaudal axis keeping parallelism of Channa argus class sample during measurement.
In described step (1), the body length in morphological parameters is the fish body kiss end distance to last piece of vertebrae end of Channa argus class sample, the cephalocaudal axis keeping parallelism of Channa argus class sample during measurement.
In described step (1), the body thickness in morphological parameters is the fish body both sides ultimate range of Channa argus class sample, the dorsoventral axis keeping parallelism of Channa argus class sample during measurement.
In described step (1), the eye footpath in morphological parameters is the eye socket internal diameter of the fish body of Channa argus class sample.
In described step (1), the eye back length in morphological parameters is the eye socket trailing edge distance to nucleus trailing edge of the fish body of Channa argus class sample, the dorsoventral axis keeping parallelism of Channa argus class sample during measurement.
Described discriminant equation is as follows:
Snapper selfing group: Y1=3882.753X1+4285.806X2-1111.759X3+2752.870X4-1957.971;
Black porgy selfing group: Y2=3944.204X1+4204.179X2-1421.639X3+3211.476X4-2032.330;
Female snapper and male black porgy hybridization group: Y3=4093.490X1+4220.458X2-1906.889X3+
2837.128X4-2084.382;
Female black porgy and male snapper hybridization group: Y4=4097.927X1+4544.774X2-2096.034X3+
3289.582X4-2169.883;
Wherein, X1 is the ratio of body length and total length, and X2 is the thick ratio with total length of body, and X3 is the ratio in a footpath and total length, and X4 is the ratio of a back length and total length.
In described step (3), the fingerling of Channa argus class sample is substitute in discriminant equation to obtain numerical value closest to the fingerling corresponding to 0.
Beneficial effect
Owing to have employed above-mentioned technical scheme, the present invention is compared with prior art, have the following advantages that and good effect: the present invention uses formalness parameter snapper, black porgy and hybrid generation thereof to be distinguished rapidly and accurately simultaneously, differentiate that rate of accuracy reached is to 99.17%, there is feature easy and simple to handle, quick, pin-point accuracy, can use manpower and material resources sparingly, very important effect is played in Channa argus class Germ-plasma resources protection and exploitation.
Accompanying drawing explanation
Fig. 1 is black porgy snapper and hybrid generation respectively measures parameter schematic diagram.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further. Should be understood that these embodiments are merely to illustrate the present invention rather than restriction the scope of the present invention. In addition, it is to be understood that after having read the content that the present invention lectures, the present invention can be made various changes or modifications by those skilled in the art, and these equivalent form of values fall within the application appended claims limited range equally.
Embodiments of the present invention relate to a kind of method differentiating black porgy, snapper and hybrid generation thereof based on morphological parameters, comprise the following steps:
1, gather fresh black porgy selfing group, snapper selfing group, female snapper and male black porgy hybridization group, female black porgy and male snapper hybridization group sample, often organize 30 tails individual;
2, following parameter is measured with slide gauge (precision 0.01cm) with reference to Figure of description:
(1) total length: kiss the end distance (shown in figure 1) to tail fin end (parallel with cephalocaudal axis);
(2) body is long: fish body kisses the end distance to last piece of vertebrae end (parallel with cephalocaudal axis), in concrete measurement, to kiss the end distance (shown in figure 2) to lateral line scales (or file squama) last scale end (parallel with cephalocaudal axis);
(3) body is thick: fish body both sides (parallel with dorsoventral axis) ultimate range (shown in figure 3);
(4) eye footpath: the eye socket internal diameter (figure shown in 4) parallel with cephalocaudal axis;
(5) eye back is long: eye socket trailing edge is to the distance (shown in figure 5) of nucleus trailing edge (parallel with cephalocaudal axis);
3, X1 (body length/total length), X2 (body thickness/total length), X3 (eye footpath/total length) and X4 (eye back length/total length) are calculated respectively;
4, black porgy selfing group, snapper selfing group, snapper (��) �� black porgy (��) hybridization group, black porgy (��) �� snapper (��) hybridization group discriminant equation are set up as follows:
Snapper selfing group: Y1=3882.753X1+4285.806X2-1111.759X3+2752.870X4-1957.971;
Black porgy selfing group: Y2=3944.204X1+4204.179X2-1421.639X3+3211.476X4-2032.330;
Female snapper and male black porgy hybridization group: Y3=4093.490X1+4220.458X2-1906.889X3+2837.128X4-2084.382;
Female black porgy and male snapper hybridization group: Y4=4097.927X1+4544.774X2-2096.034X3+3289.582X4-2169.883.
5, the Channa argus class sample morphology supplemental characteristic differentiated being needed to substitute in above-mentioned discriminant equation, gained Y value, closest to 0, is this group fingerling.
Final result, as shown in table 1, find that snapper selfing group 30 tail individuality is all referred in snapper selfing group, differentiate that accuracy rate is 100%, the differentiation accuracy rate of black porgy selfing group, female snapper and male black porgy hybridization group is also 100%, only has the 1 female black porgy of tail and is mistaken for female snapper and male black porgy hybrid generation with male snapper hybrid generation, and it differentiates that accuracy rate is 96.67%, comprehensive distinguishing rate is 99.17%, it is shown that high accuracy. Illustrate that this method is by progressively discriminatory analysis, has reached to differentiate the purpose of Channa argus class filial generation.
Table 1 snapper and black porgy selfing group and 4 combination distinguishing results of reciprocal crosses group
Claims (8)
1. the method differentiating black porgy snapper and hybrid generation thereof based on morphological parameters, it is characterised in that comprise the following steps:
(1) measuring the morphological parameters of Channa argus class sample, described morphological parameters includes: total length, body length, body thickness, eye footpath and eye back are long;
(2) discriminant equation of black porgy selfing group, snapper selfing group, female snapper and male black porgy hybridization group, female black porgy and male snapper hybridization group is set up;
(3) morphological parameters obtained is brought in discriminant equation group so that it is determined that fingerling.
2. the method differentiating black porgy snapper and hybrid generation thereof based on morphological parameters according to claim 1, it is characterized in that, in described step (1), the total length in morphological parameters is the fish body kiss end length to tail fin end of Channa argus class sample, the cephalocaudal axis keeping parallelism of Channa argus class sample during measurement.
3. the method differentiating black porgy snapper and hybrid generation thereof based on morphological parameters according to claim 1, it is characterized in that, in described step (1), the body length in morphological parameters is the fish body kiss end distance to last piece of vertebrae end of Channa argus class sample, the cephalocaudal axis keeping parallelism of Channa argus class sample during measurement.
4. the method differentiating black porgy snapper and hybrid generation thereof based on morphological parameters according to claim 1, it is characterized in that, in described step (1), the body thickness in morphological parameters is the fish body both sides ultimate range of Channa argus class sample, the dorsoventral axis keeping parallelism of Channa argus class sample during measurement.
5. the method differentiating black porgy snapper and hybrid generation thereof based on morphological parameters according to claim 1, it is characterised in that in described step (1), the eye footpath in morphological parameters is the eye socket internal diameter of the fish body of Channa argus class sample.
6. the method differentiating black porgy snapper and hybrid generation thereof based on morphological parameters according to claim 1, it is characterized in that, in described step (1), the eye back length in morphological parameters is the eye socket trailing edge distance to nucleus trailing edge of the fish body of Channa argus class sample, the dorsoventral axis keeping parallelism of Channa argus class sample during measurement.
7. the method differentiating black porgy snapper and hybrid generation thereof based on morphological parameters according to claim 1, it is characterised in that described discriminant equation is as follows:
Snapper selfing group: Y1=3882.753X1+4285.806X2-1111.759X3+2752.870X4-1957.971;
Black porgy selfing group: Y2=3944.204X1+4204.179X2-1421.639X3+3211.476X4-2032.330;
Female snapper and male black porgy hybridization group: Y3=4093.490X1+4220.458X2-1906.889X3+2837.128X4-2084.382;
Female black porgy and male snapper hybridization group: Y4=4097.927X1+4544.774X2-2096.034X3+3289.582X4-2169.883;
Wherein, X1 is the ratio of body length and total length, and X2 is the thick ratio with total length of body, and X3 is the ratio in a footpath and total length, and X4 is the ratio of a back length and total length.
8. the method differentiating black porgy snapper and hybrid generation thereof based on morphological parameters according to claim 7, it is characterised in that in described step (3), the fingerling of Channa argus class sample is substitute into obtain numerical value in discriminant equation closest to the fingerling corresponding to 0.
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| CN106119389A (en) * | 2016-08-23 | 2016-11-16 | 江苏省海洋水产研究所 | A kind of method differentiating black porgy and black porgy snapper reciprocal cross filial generation |
| CN109078861A (en) * | 2017-06-14 | 2018-12-25 | 北京市水产科学研究所 | The method for separating of Cuora flavomarginate children's tortoise figure |
| CN109258541A (en) * | 2018-11-12 | 2019-01-25 | 南京师范大学 | A method of based on ratio Characters Identification Fugu category fish sex |
| CN112741028A (en) * | 2021-01-12 | 2021-05-04 | 湖南师范大学 | Method for rapidly identifying hybrid of Megalobrama amblycephala and crucian carp and evaluating megalobrama amblycephala gynogenesis efficiency induced by red crucian carp sperm |
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Cited By (8)
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| CN106119377A (en) * | 2016-07-06 | 2016-11-16 | 中国水产科学研究院淡水渔业研究中心 | A kind of differentiate the primer of snapper, black porgy and filial generation thereof, test kit and discrimination method |
| CN106119377B (en) * | 2016-07-06 | 2019-11-29 | 中国水产科学研究院淡水渔业研究中心 | A kind of primer, kit and discrimination method identifying red porgy, black porgy and its filial generation |
| CN106119389A (en) * | 2016-08-23 | 2016-11-16 | 江苏省海洋水产研究所 | A kind of method differentiating black porgy and black porgy snapper reciprocal cross filial generation |
| CN106119389B (en) * | 2016-08-23 | 2019-07-05 | 江苏省海洋水产研究所 | A method of identifying black porgy and black porgy red porgy reciprocal cross filial generation |
| CN109078861A (en) * | 2017-06-14 | 2018-12-25 | 北京市水产科学研究所 | The method for separating of Cuora flavomarginate children's tortoise figure |
| CN109078861B (en) * | 2017-06-14 | 2020-11-27 | 北京市水产科学研究所 | Method for sorting body types of young yellow-edge cuora |
| CN109258541A (en) * | 2018-11-12 | 2019-01-25 | 南京师范大学 | A method of based on ratio Characters Identification Fugu category fish sex |
| CN112741028A (en) * | 2021-01-12 | 2021-05-04 | 湖南师范大学 | Method for rapidly identifying hybrid of Megalobrama amblycephala and crucian carp and evaluating megalobrama amblycephala gynogenesis efficiency induced by red crucian carp sperm |
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