CN105624108B - 配合淋巴细胞培养基快速诱导大量cik细胞的试剂盒及其使用方法 - Google Patents
配合淋巴细胞培养基快速诱导大量cik细胞的试剂盒及其使用方法 Download PDFInfo
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- CN105624108B CN105624108B CN201610102626.2A CN201610102626A CN105624108B CN 105624108 B CN105624108 B CN 105624108B CN 201610102626 A CN201610102626 A CN 201610102626A CN 105624108 B CN105624108 B CN 105624108B
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- 210000004698 lymphocyte Anatomy 0.000 title claims abstract description 43
- 239000001963 growth medium Substances 0.000 title claims abstract description 32
- 210000004405 cytokine-induced killer cell Anatomy 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 14
- 230000001939 inductive effect Effects 0.000 title abstract description 3
- 239000000843 powder Substances 0.000 claims abstract description 60
- 239000000243 solution Substances 0.000 claims abstract description 33
- 102000000588 Interleukin-2 Human genes 0.000 claims abstract description 20
- 108010002350 Interleukin-2 Proteins 0.000 claims abstract description 20
- 230000006698 induction Effects 0.000 claims abstract description 13
- 108010074328 Interferon-gamma Proteins 0.000 claims abstract description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 10
- 102000008070 Interferon-gamma Human genes 0.000 claims abstract description 9
- 229960003130 interferon gamma Drugs 0.000 claims abstract description 9
- 101000914514 Homo sapiens T-cell-specific surface glycoprotein CD28 Proteins 0.000 claims abstract description 7
- 102100027213 T-cell-specific surface glycoprotein CD28 Human genes 0.000 claims abstract description 7
- 101000581981 Homo sapiens Neural cell adhesion molecule 1 Proteins 0.000 claims abstract description 6
- 102100027347 Neural cell adhesion molecule 1 Human genes 0.000 claims abstract description 6
- 102000008100 Human Serum Albumin Human genes 0.000 claims abstract description 5
- 108091006905 Human Serum Albumin Proteins 0.000 claims abstract description 5
- 239000011780 sodium chloride Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000008215 water for injection Substances 0.000 claims abstract description 5
- 210000004027 cell Anatomy 0.000 claims description 36
- 239000006166 lysate Substances 0.000 claims description 20
- 239000011148 porous material Substances 0.000 claims description 12
- 238000004113 cell culture Methods 0.000 claims description 11
- 239000012930 cell culture fluid Substances 0.000 claims description 11
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 10
- 238000005374 membrane filtration Methods 0.000 claims description 10
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 7
- 239000001569 carbon dioxide Substances 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- 239000004615 ingredient Substances 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 claims description 3
- 210000005087 mononuclear cell Anatomy 0.000 claims description 3
- 239000011886 peripheral blood Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims 2
- 239000012528 membrane Substances 0.000 claims 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 7
- 239000007788 liquid Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102100037850 Interferon gamma Human genes 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000004976 peripheral blood cell Anatomy 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0646—Natural killers cells [NK], NKT cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/2302—Interleukin-2 (IL-2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/24—Interferons [IFN]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
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- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
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- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
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Priority Applications (1)
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CN201610102626.2A CN105624108B (zh) | 2016-02-24 | 2016-02-24 | 配合淋巴细胞培养基快速诱导大量cik细胞的试剂盒及其使用方法 |
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CN201610102626.2A CN105624108B (zh) | 2016-02-24 | 2016-02-24 | 配合淋巴细胞培养基快速诱导大量cik细胞的试剂盒及其使用方法 |
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CN105624108A CN105624108A (zh) | 2016-06-01 |
CN105624108B true CN105624108B (zh) | 2019-04-23 |
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CN201610102626.2A Active CN105624108B (zh) | 2016-02-24 | 2016-02-24 | 配合淋巴细胞培养基快速诱导大量cik细胞的试剂盒及其使用方法 |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101418283A (zh) * | 2007-10-23 | 2009-04-29 | 范云峰 | 一种简单高效地制备cik细胞的方法 |
CN102191219A (zh) * | 2011-03-29 | 2011-09-21 | 上海复仁生物科技有限公司 | 一种高效制备cik细胞的方法 |
CN104673751A (zh) * | 2015-03-24 | 2015-06-03 | 刘慧玉 | 一种高效cik细胞培养方法 |
-
2016
- 2016-02-24 CN CN201610102626.2A patent/CN105624108B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101418283A (zh) * | 2007-10-23 | 2009-04-29 | 范云峰 | 一种简单高效地制备cik细胞的方法 |
CN102191219A (zh) * | 2011-03-29 | 2011-09-21 | 上海复仁生物科技有限公司 | 一种高效制备cik细胞的方法 |
CN104673751A (zh) * | 2015-03-24 | 2015-06-03 | 刘慧玉 | 一种高效cik细胞培养方法 |
Non-Patent Citations (1)
Title |
---|
激发型CD28 单抗参与诱导的CIK 细胞及其表型特征;倪修文等;《中国输血杂志》;20150228;第28卷(第2期);125-128 * |
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Effective date of registration: 20220104 Address after: 300000 No. 5, Shuangchen Middle Road, Tianjin Beichen economic and Technological Development Zone, Beichen District, Tianjin (office building, room 703-018) Patentee after: Tianjin Jiexuan Biomedical Technology Co.,Ltd. Patentee after: Tianjin priiser Biotechnology Co., Ltd Address before: 300392 402A, building 4, No. 4, Haitai development second road, Huayuan Industrial Park (outside the ring), Binhai high tech Zone, Nankai District, Tianjin Patentee before: TIANJIN PURUI SAIER BIOTECHNOLOGY Co.,Ltd. |
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