CN105624087A

CN105624087A - Promoter for culturing nitrifying bacteria and preparation method of promoter

Info

Publication number: CN105624087A
Application number: CN201410585418.3A
Authority: CN
Inventors: 高会杰; 郭志华; 孙丹凤; 赵胜楠; 许谦
Original assignee: China Petroleum and Chemical Corp; Sinopec Dalian Research Institute of Petroleum and Petrochemicals
Current assignee: Sinopec Dalian Petrochemical Research Institute Co ltd; China Petroleum and Chemical Corp
Priority date: 2014-10-28
Filing date: 2014-10-28
Publication date: 2016-06-01
Anticipated expiration: 2034-10-28
Also published as: CN105624087B

Abstract

The invention discloses a promoter for culturing nitrifying bacteria. The promoter comprises the following components in parts by weight: 40-100 parts of metal salts, preferably 50-80 parts of metal salts, and 5-30 parts of polyamine substance, preferably 10-20 parts of polyamine substance, wherein the metal salts comprise calcium salt, magnesium salt and copper salt, and the molar ratio of Ca<2+> to Mg<2+> to Cu<2+> is (5-15) to (5-25) to (0.5-5), preferably (8-12) to (10-20) to (1-4). The promoter is simple in composition and easy to prepare, can be used in a culture process of nitrifying bacteria, and also can be directly fed into a sewage treatment system, so that the nitrifying bacteria can rapidly grow and breed within a short time, and the cultured nitrifying bacteria have high activity and strong impact tolerance.

Description

A kind of accelerator cultivating nitrobacteria and preparation method thereof

Technical field

The invention belongs to biological technical field, be specifically related to a kind of accelerator cultivating nitrobacteria and preparation method thereof.

Background technology

In biological denitrification method, it is no matter traditional nitrification-denitrification, or novel short-cut nitrification-denitrification and short distance nitration-anaerobic ammoxidation all need the Nitrification removal of ammonia and nitrogen through nitrobacteria. Nitrobacteria belongs to chemotrophy type microorganism. Biological cell is only with the energy preserved with forms such as ATP, it is impossible to directly utilize the free energy that chemical reaction discharges. In good oxygen metabolism, ATP synthesizes mainly through the oxidative phosphorylation of respiratory chain. Ammoxidation phosphorylation efficiency is very low, and the ATP that can be generated by is very limited, and these energy are mainly used in electron transition to higher energy level, and this makes nitrobacteria growth very slow, and the phase is 8 ~ 36h from generation to generation. In the cell wall of nitrobacteria, peptidoglycan content is low, protein and fat content are high, therefore environmental change is more sensitive, nitrobacteria adaptability natural in nature and toleration are poor, cannot get the mastery in growth competition with heterotroph microorganism under a lot of conditions. In sewage disposal system, when in activated sludge, nitrobacteria content is relatively low, rely on regulate the environmental condition such as dissolved oxygen and pH cannot within a short period of time fast-growth breeding, the sewage disposal system removal of ammonia and nitrogen ultimately resulting in existing operation is limited in one's ability, industrially generally can adopt and directly throw in cultured high concentration nitrobacteria in sewage disposal system to solve this problem. No matter it is in sewage disposal system, directly cultivate nitrobacteria or outside sewage disposal system, cultivate nitrobacteria, is required for adding the material promoting growth, the growth rate of nitrobacteria could be accelerated.

From biochemistry level, nitration reaction relates to the metabolic pathway of ammona monooxygenase, azanol oxygen also enzyme and nitrite oxidase co-catalysis, and converts along with complicated matter and energy. Adopt Biostimulation agent to improve the activity of these enzymes in nitrobacteria, be then solve one of slow effective way of nitrobacteria growth, nitration reaction process can be accelerated simultaneously. A lot of about the research of Biostimulation agent at present, CN200510111874.5, CN200510111876.4, CN200510111877.9 and CN200510111875.X propose the nitrobacteria growth promoter utilizing different metal salt combination respectively, and main component includes molasses, slaine (iron salt, manganese salt, calcium salt and magnesium salt) and adsorbent. After using this accelerator, ammonia nitrogen removal frank can improve more than 20%. But be mainly the materials such as zeolite powder, kieselguhr, Powdered Activated Carbon or flyash due to adsorbent, adding of these adsorbents will certainly increase sludge yield. CN201110315549.6 discloses the cultural method of a kind of short-cut nitrification and denitrification granular sludge, it is characterized in that regularly adding 5 ~ 15mg/L azanol; CN201010168453.7 discloses a kind of method of quickly starting oxygen-poor ammonia oxidizing biofilter, it is characterized in that step (2) adds azanol, challenge inoculation mud changes to oxygen-poor ammonia oxidizing biomembrane, more than invent mainly by the azanol inhibitory action to NOB, promote short distance nitration effect. CN201410141638.7 discloses the culture fluid of a kind of nitrite bacteria and preparation and cultural method, its culture fluid formula is more complicated, the element of Growth of Cells is provided except relating to ferrum, calcium, potassium, magnesium etc., further relates to cobalt, molybdenum, manganese, zinc etc. and promote the element of enzymatic synthesis.

Summary of the invention

For the deficiencies in the prior art, the invention provides a kind of accelerator cultivating nitrobacteria and preparation method thereof. This accelerator formula is simple, preparation is easily, it is possible to in the incubation of nitrobacteria, it is also possible to be directly added in sewage disposal system, making nitrobacteria to breed by fast-growth at short notice, the nitrobacteria activity cultivated is high, tolerance impact capacity is strong.

The present invention cultivates the accelerator of nitrobacteria, and including slaine and polyamines, with parts by weight, slaine is 40 ~ 100 weight portions, it is preferred to 50 ~ 80 weight portions; Polyamines is 5 ~ 30 weight portions, it is preferred to 10 ~ 20 weight portions; Described slaine is calcium salt, magnesium salt and mantoquita, Ca²⁺��Mg²⁺And Cu²⁺Mol ratio be (5 ~ 15): (5 ~ 25): (0.5 ~ 5), it is preferred to (8 ~ 12): (10 ~ 20): (1 ~ 4).

Calcium salt of the present invention is CaSO₄Or CaCl₂, it is preferable that CaSO₄; Magnesium salt is MgSO₄Or MgCl₂, it is preferable that MgSO₄; Mantoquita is CuSO₄Or CuCl₂, it is preferable that CuSO₄��

Polyamines of the present invention is spermine, spermidine or both mixture.

Accelerator of the present invention also includes mineral acid hydroxylamines, and content is 0.5 ~ 15 weight portion, it is preferred to 2 ~ 10 weight portions. Described mineral acid hydroxylamines is one or more in oxammonium hydrochloride., oxammonium sulfate. or phosphatic hydroxylamine, it is preferred to oxammonium sulfate..

The preparation method of the accelerator of cultivation nitrobacteria of the present invention, including following content: (1) prepares metal salt solution according to consisting of and weight portion: slaine is 40 ~ 100 weight portions, being preferably 50 ~ 80 weight portions, described slaine is calcium salt, magnesium salt and mantoquita, Ca in slaine²⁺��Mg²⁺And Cu²⁺Mol ratio be (5 ~ 15): (5 ~ 25): (0.5 ~ 5), it is preferred to (8 ~ 12): (10 ~ 20): (1 ~ 4); (2) by 5 ~ 30 weight portions before using, it is preferred to the polyamines of 10 ~ 20 weight portions joins in metal salt solution.

Calcium salt described in step of the present invention (1) is CaSO₄Or CaCl₂, it is preferable that CaSO₄; Magnesium salt is MgSO₄Or MgCl₂, it is preferable that MgSO₄; Mantoquita is CuSO₄Or CuCl₂, it is preferable that CuSO₄��

Polyamines described in step of the present invention (2) is spermine, spermidine or both mixture.

Further, can also add content before using in step of the present invention (2) is 0.5 ~ 15 weight portion, being preferably the mineral acid azanol of 2 ~ 10 weight portions, described mineral acid azanol is one or more in oxammonium hydrochloride., oxammonium sulfate. or phosphatic hydroxylamine, it is preferred to oxammonium sulfate..

Nitrobacteria growth promoter of the present invention may be used for the incubation of nitrobacteria, it is possible to for improving the ammonia nitrogen removal effect of sewage disposal system, it is also possible to for quickly starting and the fast quick-recovery of the system that is hit containing ammonia system. Concrete needs determines dosage according to sewage character, treating capacity and treatment effect. In use, first accelerator is dissolved, then according to promoter concentration 10 ~ 100mg/L adds in sewage.

Compared with prior art, the method have the advantages that

1, utilize the metal ion that slaine provides to grow required element as nitrobacteria, simultaneously as the activity of the component raising enzyme of enzyme, it is possible to fast degradation substrate, accelerate enzymatic reaction process. Polyamines and metal ion combined effect, it is possible to accelerate cell proliferation, and settleability and the stability of gather in the crops thalline can be improved, prolongation thalline service life.

2, can directly participating in the metabolic process of nitrobacteria as the substrate of azanol oxygen also enzyme, shorten enzymatic reaction process appropriate addition of mineral acid azanol, the activator simultaneously as cell can accelerated cell growth.

3, the present invention is by selecting component and the proportioning of nitrobacteria growth promoter, under the combined effect of slaine, polyamines and mineral acid azanol, it is achieved the purpose of fast culture nitrobacteria. The invention solves the problem that nitrobacteria growth is slow, may also speed up nitration reaction process simultaneously, improve the treatment effect of waste water. After using accelerator, the growth rate of nitrobacteria can increase by 10 ~ 100 times, and the ammonia nitrogen removal frank of ammonia-containing water can improve more than 30%, moreover it is possible to solves the fast recovery problem of the nitrated ammonia nitrogen removal ability of sewage disposal system being hit well.

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention program is described in detail.

The formula of the nitrobacteria growth promoter of embodiment 1 ~ 8 and comparative example 1-4 is in Table 1.

The formula of table 1 accelerator and ratio

The major pollutants COD concentration contained in the waste water of certain enterprise discharge is about 1000mg/L, ammonia nitrogen concentration is about 150mg/L, this factory's anaerobic-aerobic (A/O) technique processes, but in water outlet after treatment, COD concentration is still up to more than 60mg/L less than 60mg/L ammonia nitrogen concentration. The accelerator that in utilization, table proportioning configures, configuration concentration is 0.5g/L, every day is that 30mg/L adds according to promoter concentration in sewage, after adding 15 days, ammonia nitrogen removal frank improves more than 10% stopping and adding, system continues to run with 15 days, and water sampling is analyzed water outlet ammonia nitrogen concentration and calculates ammonia nitrogen removal frank. After adding, concrete result is in Table 2, effluent quality from each embodiment of table 2, when adding accelerator, water outlet ammonia nitrogen concentration is 75mg/L, ammonia nitrogen removal frank only has 50%, and after adding accelerator, water outlet ammonia nitrogen concentration is below 30mg/L, and ammonia nitrogen removal frank is all higher than 80%, during when accelerator containing only the slaine having in formula or polyamines, water outlet ammonia nitrogen concentration still between 30 ~ 50mg/L, ammonia nitrogen removal frank is lower than 80%. As can be seen here, the growth promoter of the present invention is used can to significantly improve the clearance of ammonia nitrogen in sewage.

Table 2 adopts the treatment effect of different formulations accelerator

Example	Water outlet ammonia nitrogen concentration, mg/L	Ammonia nitrogen removal frank, %	Sludge settling ratio SV₃₀, %
				Embodiment 1	22.5	85.0	24
Embodiment 2	26.0	82.7	28
				Embodiment 3	28.1	81.3	30
Embodiment 4	23.7	84.2	29
				Embodiment 5	29.9	80.1	27
Embodiment 6	24.5	83.7	33
				Embodiment 7	14.3	90.5	20
Embodiment 8	17.4	88.4	23
				Comparative example 1	35.0	76.7	38
Comparative example 2	38.9	74.1	36
				Comparative example 3	45.3	69.8	40
Comparative example 4	41.3	72.5	37
				It is not added with accelerator	75.0	50.0	48

After process terminates, Aeration tank active sludge intermixture is carried out sludge settling ratio SV₃₀Detection, result is as shown in table 2, illustrates that growth promoter of the present invention contributes to mud and better settles.

Claims

1. the accelerator cultivating nitrobacteria, it is characterised in that include slaine and polyamines, with parts by weight, slaine is 40 ~ 100 weight portions, and polyamines is 5 ~ 30 weight portions; Described slaine is calcium salt, magnesium salt and mantoquita, Ca²⁺��Mg²⁺And Cu²⁺Mol ratio be (5 ~ 15): (5 ~ 25): (0.5 ~ 5).

2. the growth promoter described in claim 1, it is characterised in that: slaine is 50 ~ 80 weight portions, and polyamines is 10 ~ 20 weight portions, Ca²⁺��Mg²⁺And Cu²⁺Mol ratio be (8 ~ 12): (10 ~ 20): (1 ~ 4).

3. the growth promoter described in claim 1 or 2, it is characterised in that: calcium salt is CaSO₄Or CaCl₂, magnesium salt is MgSO₄Or MgCl₂, mantoquita is CuSO₄Or CuCl₂��

4. the growth promoter described in claim 1 or 2, it is characterised in that: calcium salt is CaSO₄, magnesium salt is MgSO₄, mantoquita is CuSO₄��

5. the growth promoter described in claim 1 or 2, it is characterised in that: polyamines is spermine, spermidine or both mixture.

6. the growth promoter described in claim 1 or 2, it is characterised in that: described accelerator also includes mineral acid azanol, and content is 0.5 ~ 15 weight portion.

7. the growth promoter described in claim 6, it is characterised in that: mineral acid azanol is one or more in oxammonium hydrochloride., oxammonium sulfate. or phosphatic hydroxylamine, and content is 2 ~ 10 weight portions.

8. the growth promoter described in claim 6, it is characterised in that: mineral acid hydroxylamines is oxammonium sulfate..

9. the preparation method of the accelerator cultivating nitrobacteria, it is characterized in that including following content: (1) prepares metal salt solution according to consisting of and weight portion: slaine is 40 ~ 100 weight portions, it is preferably 50 ~ 80 weight portions, described slaine is calcium salt, magnesium salt and mantoquita, Ca in slaine²⁺��Mg²⁺And Cu²⁺Mol ratio be (5 ~ 15): (5 ~ 25): (0.5 ~ 5), it is preferred to (8 ~ 12): (10 ~ 20): (1 ~ 4); (2) by 5 ~ 30 weight portions before using, it is preferred to the polyamines of 10 ~ 20 weight portions joins in metal salt solution.

10. in accordance with the method for claim 9, it is characterised in that: it is additionally added, before step (2) uses, the mineral acid azanol that content is 0.5 ~ 15 weight portion, it is preferred to 2 ~ 10 weight portions; Mineral acid azanol is one or more in oxammonium hydrochloride., oxammonium sulfate. or phosphatic hydroxylamine.

11. the application of the arbitrary described accelerator of claim 1-10, it is characterised in that: in use, first accelerator is dissolved, then according to promoter concentration 10 ~ 100mg/L adds in sewage.