CN105617595A - Method for preparing pure biomass organophosphorus pesticide degradation agent - Google Patents
Method for preparing pure biomass organophosphorus pesticide degradation agent Download PDFInfo
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- CN105617595A CN105617595A CN201610151238.3A CN201610151238A CN105617595A CN 105617595 A CN105617595 A CN 105617595A CN 201610151238 A CN201610151238 A CN 201610151238A CN 105617595 A CN105617595 A CN 105617595A
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/10—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by subjecting to electric or wave energy or particle or ionizing radiation
- A62D3/17—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by subjecting to electric or wave energy or particle or ionizing radiation to electromagnetic radiation, e.g. emitted by a laser
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/30—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by reacting with chemical agents
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/04—Pesticides, e.g. insecticides, herbicides, fungicides or nematocides
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/26—Organic substances containing nitrogen or phosphorus
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2203/00—Aspects of processes for making harmful chemical substances harmless, or less harmful, by effecting chemical change in the substances
- A62D2203/02—Combined processes involving two or more distinct steps covered by groups A62D3/10 - A62D3/40
Abstract
The invention discloses a method for preparing a pure biomass organophosphorus pesticide degradation agent and belongs to the field of pesticide degradation agents. According to the method, a large number of organophosphorus pesticide degrading bacteria are cultured, separated and screened out from mixed soil of earth surface sludge, activated sludge and common earth surface soil; hypha suspension liquid, humus and chitosan are mixed and aged to obtain a pasty mixture; and the pasty mixture, nano titanium dioxide and a garlic extract concentrated solution are mixed and pelletized. The organophosphorus pesticide degrading bacteria screened and acclimatized through the method have high degradation activity, and the temperature and pH applicable range is wide; the pure biomass organophosphorus pesticide degradation agent is combined with substances such as humic acid and can degrade organophosphorus pesticide more thoroughly and faster; allicin and alliin in the garlic extract concentrated solution have a degradation effect on the organophosphorus pesticide; degradation of the pesticide can be accelerated through photocatalysis of the nano titanium dioxide; moreover, the prepared pure biomass degradation agent is non-toxic, has no residues and is free of secondary pollution.
Description
Technical field
The present invention discloses the preparation method of a kind of pure biomass organic phosphorus pesticide degradation agent, belongs to degradation of pesticide agent field.
Background technology
Big quantifier elimination shows, the degradation rate using certain agricultural chemicals can cause this kind of agricultural chemicals in soil continuously is accelerated. People have confirmed to cause the reason of this kind of phenomenon mainly to cause by the microorganism of degradable agricultural chemicals, and the ability of these a microbiological deterioration agricultural chemicals part is intrinsic, and a part brings out generation after using agricultural chemicals. Soil microorganisms system comprises bacterium, fungi, actinomycetes, algae etc., and agricultural chemicals is had Degradation by them. Bacterium is due to the multiple adaptive faculty on its biochemistry and easy Mutation induction bacterial strain, thus occupies main status in the microorganism of degrading pesticide. Degradation of pesticide bacterium in soil the microorganism of no special, they are widely distributed in soil. Further, certain agricultural chemicals often has multiple degradation bacteria simultaneously, and Multiple Pesticides also can be had Degradation by same bacterium.
The compound composition processing dense chemicals contamination environment is very unstable, often fluctuates, and the growth for pesticide degradation bacteria is very unfavorable, and temperature, pH and moisture fluctuation are also bigger, it is possible to suppress the growth of degradation bacteria; From environment, directly screen the pesticide degradation bacteria degradation speed obtained slow, actual needs can not be met or often morph cause degradation capability forfeiture, can not continue degrading pesticide. In addition, render to the impact that the degradation bacteria gone in environment also can be subject to the original bacterium group of this environment, be even subject to tangerine anti-and can not long-term survival in this context, pesticide degradation bacteria can not become dominant bacteria etc. in contaminated environment.
Summary of the invention
The technical problem that the present invention mainly solves: at present directly from environment screening to obtain biomass pesticides degradation bacteria degradation speed slow, temperature, pH and moisture fluctuation are also bigger, often morph and cause the forfeiture of degradation capability, can not continue the problem of degrading pesticide, provide the preparation method of a kind of pure biomass organic phosphorus pesticide degradation agent, the present invention is from earth's surface mud, the mixed soil of active sludge and normal surface soil is cultivated, separate a large amount of organophosphorus pesticide degradation bacterium, mycelia suspension is mixed with soil ulmin and chitosan, ageing obtains pasty mixture, pasty mixture and nano titanium oxide and garlic are extracted concentrated solution mixing, granulation, , the present invention's screening and the rear organophosphorus pesticide degradation bacterium of domestication have higher degrading activity, to temperature and pH wide accommodation, with combinations of substances such as humic acid, organic phosphorus pesticide degradation is more thorough, degradation speed is fast, wherein organophosphorus pesticide is had Degradation by the garlicin in garlic extraction concentrated solution and alliin, the photocatalytic of nano titanium oxide also can accelerate the degraded of agricultural chemicals, and obtained pure biomass degradation agent is nontoxic, noresidue, non-secondary pollution.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:
1.(1) take the earth's surface mud of 200��300g pesticide producing factory, the active sludge of 200��300g sewage work and 300��400g normal surface soil respectively, three kinds of earth are mixed, get 4��5g mixed soil, joined in 100mL minimal medium;
2.(2) measuring the organophosphorus pesticide that 40��50mL concentration is 50��100mg/L adds in above-mentioned minimal medium, it is placed in thermostat container, quiescent culture at 25��35 DEG C, and by inoculum size 15%, mixed soil was re-seeded in fresh minimal medium every 5��7 days, increase the content of organophosphorus pesticide during transfer inoculation with the speed of 50��100mg/L, until its concentration reaches 400mg/L every time;
3.(3) maintain machine phosphorus insecticide concentration at 400mg/L, repeat above-mentioned steps switching and cultivate domestication 1��2 month, inorganic salt surface growth it is chosen at the fastest after domestication, the bacterium colony that area is maximum, choose with transfering loop and get this bacterium colony, transfer on enrichment medium, cultivate 3��5 days at continuing at 40��50 DEG C;
4.(4) cultivation is surperficial with 1��2L flowing aseptic water washing substratum mycelia after terminating, collect leacheate, obtain domestication mycelia suspension, taking 400��500g soil ulmin joins in domestication mycelia suspension, constantly stirring by stirring rod, adding 50��60mL concentration at the process resume stirred is 100mg/L chitosan solution, puts into ageing drum, left at room temperature ageing is spent the night, and obtains pasty mixture for subsequent use;
5.(5) take 100��200g garlic, stone mortar is put into after peeling, mashed garlic is smashed into pieces with stone pestle, it is that mashed garlic and dehydrated alcohol are mixed by 1:5 by solid-to-liquid ratio again, puts into supersound extraction instrument, supersound extraction 30��40min under 100��200W power, filter to get filtrate, obtain supernatant liquor with supercentrifuge with 4000��5000r/min speed centrifugation, revolve finally by rotatory evaporator and boil off except unnecessary alcohol, obtain garlic and extract concentrated solution;
6.(6) measure 30��40mL garlic to extract concentrated solution and 3��5g nano titanium dioxide powder and join in 200��300g step (4) pasty mixture for subsequent use; move into after mixing in feed granulator; the obtained particle diameter of compacting granulation is 3��5mm particle, namely obtains pure biomass organic phosphorus pesticide degradation agent after packaging.
Described minimal medium is made up of 0.1��0.2% potassium primary phosphate, 0.3��0.5% dipotassium hydrogen phosphate, 0.1��0.2% magnesium sulfate, 0.05��0.08% sodium-chlor, 0.1��0.3% calcium chloride and 99.0��99.5% distilled water by mass percentage.
Described enrichment medium is made up of 40��50% peptones, 0.5��1% yeast extract, 10��15% mashed potatos, 3��5% sodium-chlor and 40��50% distilled water by mass percentage.
The method of the application of the present invention: pure biomass organic phosphorus pesticide degradation agent the present invention obtained is mixed to particle by solid-to-liquid ratio 1:10 and tap water and dissolves completely, solution atomizer is evenly sprayed at and sprays on the farm crop of acephate pesticide after 8��10 days, the amount of the front acephate of sprinkling on farm crop is 0.92��0.96mg/kg, sprayed rear farm crop carry out detect acephate residual quantity be 0.02��0.04mg/kg, degradation of pesticide effect is remarkable.
The invention has the beneficial effects as follows:
(1) the present invention's screening and the rear organophosphorus pesticide degradation bacterium of domestication have higher degrading activity, to temperature and pH wide accommodation;
(2) the present invention is by the combinations of substances such as organophosphorus pesticide degradation bacterium and humic acid, to organic phosphorus pesticide degradation more thoroughly, degradation speed fast, obtained pure biomass degradation agent is nontoxic, noresidue, non-secondary pollution;
(3) garlic that the present invention adds is extracted garlicin and alliin in concentrated solution and organophosphorus pesticide is had Degradation.
Embodiment
First the earth's surface mud of 200��300g pesticide producing factory, the active sludge of 200��300g sewage work and 300��400g normal surface soil is taken respectively, three kinds of earth are mixed, get 4��5g mixed soil, joined in 100mL minimal medium; Measuring the organophosphorus pesticide that 40��50mL concentration is 50��100mg/L adds in above-mentioned minimal medium, it is placed in thermostat container, quiescent culture at 25��35 DEG C, and by inoculum size 15%, mixed soil was re-seeded in fresh minimal medium every 5��7 days, increase the content of organophosphorus pesticide during transfer inoculation with the speed of 50��100mg/L, until its concentration reaches 400mg/L every time; Maintaining machine phosphorus insecticide concentration at 400mg/L, domestication 1��2 month is cultivated in repeating step switching, is chosen at inorganic salt surface growth the fastest after domestication, the bacterium colony that area is maximum, choose with transfering loop and get this bacterium colony, transfer on enrichment medium, cultivate 3��5 days at continuing at 40��50 DEG C; Cultivation is surperficial with 1��2L flowing aseptic water washing substratum mycelia after terminating, collect leacheate, obtain domestication mycelia suspension, taking 400��500g soil ulmin joins in domestication mycelia suspension, constantly stirring by stirring rod, adding 50��60mL concentration at the process resume stirred is 100mg/L chitosan solution, puts into ageing drum, left at room temperature ageing is spent the night, and obtains pasty mixture for subsequent use; Take 100��200g garlic, stone mortar is put into after peeling, mashed garlic is smashed into pieces with stone pestle, it is that mashed garlic and dehydrated alcohol are mixed by 1:5 by solid-to-liquid ratio again, puts into supersound extraction instrument, supersound extraction 30��40min under 100��200W power, filter to get filtrate, obtain supernatant liquor with supercentrifuge with 4000��5000r/min speed centrifugation, revolve finally by rotatory evaporator and boil off except unnecessary alcohol, obtain garlic and extract concentrated solution; Measure 30��40mL garlic to extract concentrated solution and 3��5g nano titanium dioxide powder and join in 200��300g pasty mixture for subsequent use; move into after mixing in feed granulator; the obtained particle diameter of compacting granulation is 3��5mm particle, namely obtains pure biomass organic phosphorus pesticide degradation agent after packaging.
Described minimal medium is made up of 0.1��0.2% potassium primary phosphate, 0.3��0.5% dipotassium hydrogen phosphate, 0.1��0.2% magnesium sulfate, 0.05��0.08% sodium-chlor, 0.1��0.3% calcium chloride and 99.0��99.5% distilled water by mass percentage.
Described enrichment medium is made up of 40��50% peptones, 0.5��1% yeast extract, 10��15% mashed potatos, 3��5% sodium-chlor and 40��50% distilled water by mass percentage.
Example 1
13. take the earth's surface mud of 200g pesticide producing factory, the active sludge of 200g sewage work and 300g normal surface soil first respectively, are mixed by three kinds of earth, get 4g mixed soil, are joined in 100mL minimal medium; Measuring the organophosphorus pesticide that 40mL concentration is 50mg/L adds in above-mentioned minimal medium, it is placed in thermostat container, quiescent culture at 25 DEG C, and by inoculum size 15%, mixed soil was re-seeded in fresh minimal medium every 5 days, increase the content of organophosphorus pesticide during transfer inoculation with the speed of 50mg/L, until its concentration reaches 400mg/L every time; Maintaining machine phosphorus insecticide concentration at 400mg/L, domestication 1 month is cultivated in repeating step switching, is chosen at inorganic salt surface growth the fastest after domestication, and the bacterium colony that area is maximum, chooses with transfering loop and get this bacterium colony, transfers on enrichment medium, cultivates 3 days at continuing at 40 DEG C; Cultivation is surperficial with 1L flowing aseptic water washing substratum mycelia after terminating, collect leacheate, obtain domestication mycelia suspension, taking 400g soil ulmin joins in domestication mycelia suspension, constantly stirring by stirring rod, adding 50mL concentration at the process resume stirred is 100mg/L chitosan solution, puts into ageing drum, left at room temperature ageing is spent the night, and obtains pasty mixture for subsequent use; Take 100g garlic, stone mortar is put into after peeling, mashed garlic is smashed into pieces with stone pestle, it is that mashed garlic and dehydrated alcohol are mixed by 1:5 by solid-to-liquid ratio again, puts into supersound extraction instrument, supersound extraction 30min under 100W power, filter to get filtrate, obtain supernatant liquor with supercentrifuge with 4000r/min speed centrifugation, revolve finally by rotatory evaporator and boil off except unnecessary alcohol, obtain garlic and extract concentrated solution; Measure 30mL garlic to extract concentrated solution and 3g nano titanium dioxide powder and join in 200g pasty mixture for subsequent use; move into after mixing in feed granulator; the obtained particle diameter of compacting granulation is 3mm particle, namely obtains pure biomass organic phosphorus pesticide degradation agent after packaging.
Described minimal medium is made up of 0.1% potassium primary phosphate, 0.3% dipotassium hydrogen phosphate, 0.1% magnesium sulfate, 0.05% sodium-chlor, 0.1% calcium chloride and 99.0% distilled water by mass percentage.
Described enrichment medium is made up of 40% peptone, 0.5% yeast extract, 10% mashed potato, 3% sodium-chlor and 40% distilled water by mass percentage.
Pure biomass organic phosphorus pesticide degradation agent the present invention obtained is mixed to particle by solid-to-liquid ratio 1:10 and tap water and dissolves completely, solution atomizer is evenly sprayed at and sprays on the farm crop of acephate pesticide after 8 days, the amount of the front acephate of sprinkling on farm crop is 0.92mg/kg, sprayed rear farm crop carry out detect acephate residual quantity be 0.02mg/kg, degradation of pesticide effect is remarkable.
Example 2
17. take the earth's surface mud of 250g pesticide producing factory, the active sludge of 250g sewage work and 350g normal surface soil first respectively, are mixed by three kinds of earth, get 4.5g mixed soil, are joined in 100mL minimal medium; Measuring the organophosphorus pesticide that 45mL concentration is 75mg/L adds in above-mentioned minimal medium, it is placed in thermostat container, quiescent culture at 30 DEG C, and by inoculum size 15%, mixed soil was re-seeded in fresh minimal medium every 6 days, increase the content of organophosphorus pesticide during transfer inoculation with the speed of 75mg/L, until its concentration reaches 400mg/L every time; Maintaining machine phosphorus insecticide concentration at 400mg/L, domestication 1.5 months is cultivated in repeating step switching, is chosen at inorganic salt surface growth the fastest after domestication, the bacterium colony that area is maximum, choose with transfering loop and get this bacterium colony, transfer on enrichment medium, cultivate 4 days at continuing at 45 DEG C; Cultivation is surperficial with 1.5L flowing aseptic water washing substratum mycelia after terminating, collect leacheate, obtain domestication mycelia suspension, taking 450g soil ulmin joins in domestication mycelia suspension, constantly stirring by stirring rod, adding 55mL concentration at the process resume stirred is 100mg/L chitosan solution, puts into ageing drum, left at room temperature ageing is spent the night, and obtains pasty mixture for subsequent use; Take 150g garlic, stone mortar is put into after peeling, mashed garlic is smashed into pieces with stone pestle, it is that mashed garlic and dehydrated alcohol are mixed by 1:5 by solid-to-liquid ratio again, puts into supersound extraction instrument, supersound extraction 35min under 150W power, filter to get filtrate, obtain supernatant liquor with supercentrifuge with 4500r/min speed centrifugation, revolve finally by rotatory evaporator and boil off except unnecessary alcohol, obtain garlic and extract concentrated solution; Measure 35mL garlic to extract concentrated solution and 4g nano titanium dioxide powder and join in 250g pasty mixture for subsequent use; move into after mixing in feed granulator; the obtained particle diameter of compacting granulation is 4mm particle, namely obtains pure biomass organic phosphorus pesticide degradation agent after packaging.
Described minimal medium is made up of 0.15% potassium primary phosphate, 0.4% dipotassium hydrogen phosphate, 0.15% magnesium sulfate, 0.07% sodium-chlor, 0.2% calcium chloride and 99.3% distilled water by mass percentage.
Described enrichment medium is made up of 45% peptone, 0.6% yeast extract, 13% mashed potato, 4% sodium-chlor and 45% distilled water by mass percentage.
Pure biomass organic phosphorus pesticide degradation agent the present invention obtained is mixed to particle by solid-to-liquid ratio 1:10 and tap water and dissolves completely, solution atomizer is evenly sprayed at and sprays on the farm crop of acephate pesticide after 9 days, the amount of the front acephate of sprinkling on farm crop is 0.94mg/kg, sprayed rear farm crop carry out detect acephate residual quantity be 0.03mg/kg, degradation of pesticide effect is remarkable.
Example 3
21. take the earth's surface mud of 300g pesticide producing factory, the active sludge of 300g sewage work and 400g normal surface soil first respectively, are mixed by three kinds of earth, get 5g mixed soil, are joined in 100mL minimal medium; Measuring the organophosphorus pesticide that 50mL concentration is 100mg/L adds in above-mentioned minimal medium, it is placed in thermostat container, quiescent culture at 35 DEG C, and by inoculum size 15%, mixed soil was re-seeded in fresh minimal medium every 7 days, increase the content of organophosphorus pesticide during transfer inoculation with the speed of 100mg/L, until its concentration reaches 400mg/L every time; Maintaining machine phosphorus insecticide concentration at 400mg/L, domestication 2 months is cultivated in repeating step switching, is chosen at inorganic salt surface growth the fastest after domestication, and the bacterium colony that area is maximum, chooses with transfering loop and get this bacterium colony, transfers on enrichment medium, cultivates 5 days at continuing at 50 DEG C; Cultivation is surperficial with 2L flowing aseptic water washing substratum mycelia after terminating, collect leacheate, obtain domestication mycelia suspension, taking 500g soil ulmin joins in domestication mycelia suspension, constantly stirring by stirring rod, adding 60mL concentration at the process resume stirred is 100mg/L chitosan solution, puts into ageing drum, left at room temperature ageing is spent the night, and obtains pasty mixture for subsequent use; Take 200g garlic, stone mortar is put into after peeling, mashed garlic is smashed into pieces with stone pestle, it is that mashed garlic and dehydrated alcohol are mixed by 1:5 by solid-to-liquid ratio again, puts into supersound extraction instrument, supersound extraction 40min under 200W power, filter to get filtrate, obtain supernatant liquor with supercentrifuge with 5000r/min speed centrifugation, revolve finally by rotatory evaporator and boil off except unnecessary alcohol, obtain garlic and extract concentrated solution; Measure 40mL garlic to extract concentrated solution and 3��5g nano titanium dioxide powder and join in 300g pasty mixture for subsequent use; move into after mixing in feed granulator; the obtained particle diameter of compacting granulation is 5mm particle, namely obtains pure biomass organic phosphorus pesticide degradation agent after packaging.
Described minimal medium is made up of 0.2% potassium primary phosphate, 0.5% dipotassium hydrogen phosphate, 0.2% magnesium sulfate, 0.08% sodium-chlor, 0.3% calcium chloride and 99.5% distilled water by mass percentage.
Described enrichment medium is made up of 50% peptone, 1% yeast extract, 15% mashed potato, 5% sodium-chlor and 50% distilled water by mass percentage.
Pure biomass organic phosphorus pesticide degradation agent the present invention obtained is mixed to particle by solid-to-liquid ratio 1:10 and tap water and dissolves completely, solution atomizer is evenly sprayed at and sprays on the farm crop of acephate pesticide after 10 days, the amount of the front acephate of sprinkling on farm crop is 0.96mg/kg, sprayed rear farm crop carry out detect acephate residual quantity be 0.04mg/kg, degradation of pesticide effect is remarkable.
Claims (3)
1. the preparation method of a pure biomass organic phosphorus pesticide degradation agent, it is characterised in that concrete preparation process is:
(1) the earth's surface mud of 200��300g pesticide producing factory, the active sludge of 200��300g sewage work and 300��400g normal surface soil is taken respectively, three kinds of earth are mixed, get 4��5g mixed soil, joined in 100mL minimal medium;
(2) measuring the organophosphorus pesticide that 40��50mL concentration is 50��100mg/L adds in above-mentioned minimal medium, it is placed in thermostat container, quiescent culture at 25��35 DEG C, and by inoculum size 15%, mixed soil was re-seeded in fresh minimal medium every 5��7 days, increase the content of organophosphorus pesticide during transfer inoculation with the speed of 50��100mg/L, until its concentration reaches 400mg/L every time;
(3) maintain machine phosphorus insecticide concentration at 400mg/L, repeat above-mentioned steps switching and cultivate domestication 1��2 month, be chosen at inorganic salt surface growth after domestication the fastest, the bacterium colony that area is maximum, choose with transfering loop and get this bacterium colony, transfer on enrichment medium, cultivate 3��5 days at continuing at 40��50 DEG C;
(4) cultivation is surperficial with 1��2L flowing aseptic water washing substratum mycelia after terminating, collect leacheate, obtain domestication mycelia suspension, taking 400��500g soil ulmin joins in domestication mycelia suspension, constantly stirring by stirring rod, adding 50��60mL concentration at the process resume stirred is 100mg/L chitosan solution, puts into ageing drum, left at room temperature ageing is spent the night, and obtains pasty mixture for subsequent use;
(5) 100��200g garlic is taken, stone mortar is put into after peeling, mashed garlic is smashed into pieces with stone pestle, it is that mashed garlic and dehydrated alcohol are mixed by 1:5 by solid-to-liquid ratio again, puts into supersound extraction instrument, supersound extraction 30��40min under 100��200W power, filter to get filtrate, obtain supernatant liquor with supercentrifuge with 4000��5000r/min speed centrifugation, revolve finally by rotatory evaporator and boil off except unnecessary alcohol, obtain garlic and extract concentrated solution;
(6) measure 30��40mL garlic to extract concentrated solution and 3��5g nano titanium dioxide powder and join in 200��300g step (4) pasty mixture for subsequent use; move into after mixing in feed granulator; the obtained particle diameter of compacting granulation is 3��5mm particle, namely obtains pure biomass organic phosphorus pesticide degradation agent after packaging.
2. the preparation method of a kind of pure biomass organic phosphorus pesticide degradation agent according to claim 1, it is characterised in that: described minimal medium is made up of 0.1��0.2% potassium primary phosphate, 0.3��0.5% dipotassium hydrogen phosphate, 0.1��0.2% magnesium sulfate, 0.05��0.08% sodium-chlor, 0.1��0.3% calcium chloride and 99.0��99.5% distilled water by mass percentage.
3. the preparation method of a kind of pure biomass organic phosphorus pesticide degradation agent according to claim 1, it is characterised in that: described enrichment medium is made up of 40��50% peptones, 0.5��1% yeast extract, 10��15% mashed potatos, 3��5% sodium-chlor and 40��50% distilled water by mass percentage.
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| CN201610151238.3A CN105617595A (en) | 2016-03-17 | 2016-03-17 | Method for preparing pure biomass organophosphorus pesticide degradation agent |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107410385A (en) * | 2017-04-21 | 2017-12-01 | 王现明 | A kind of method for producing insecticide for watermelon |
| CN112940732A (en) * | 2021-02-18 | 2021-06-11 | 陕西省微生物研究所 | Soil organic phosphorus pesticide degradation catalyst and preparation method thereof |
-
2016
- 2016-03-17 CN CN201610151238.3A patent/CN105617595A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107410385A (en) * | 2017-04-21 | 2017-12-01 | 王现明 | A kind of method for producing insecticide for watermelon |
| CN112940732A (en) * | 2021-02-18 | 2021-06-11 | 陕西省微生物研究所 | Soil organic phosphorus pesticide degradation catalyst and preparation method thereof |
| CN112940732B (en) * | 2021-02-18 | 2022-02-08 | 陕西省微生物研究所 | Soil organic phosphorus pesticide degradation catalyst and preparation method thereof |
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