CN105617175B

CN105617175B - A kind of Chinese medicine composition and preparation method thereof for treating gynecological disease

Info

Publication number: CN105617175B
Application number: CN201410597884.3A
Authority: CN
Inventors: 张保献
Original assignee: Beijing Kerui Pharmaceutical Ltd By Share Ltd Profit Innovation
Current assignee: JIANGXI BAISHEN CHANGNUO PHARMACEUTICAL Co.,Ltd.
Priority date: 2014-10-31
Filing date: 2014-10-31
Publication date: 2019-08-06
Anticipated expiration: 2034-10-31
Also published as: CN105617175A

Abstract

The invention discloses a kind of Chinese medicine compositions and preparation method thereof for treating gynecological disease.The Chinese medicine composition includes that radix gentianae, oldenlandia diffusa, the tuber of stemona, Rhizoma Chuanxiong, cortex dictamni etc. are prepared according to a certain weight ratio.It can be made into any common dosage forms.The traditional chinese medicine composition of the invention has the effect of clearing heat and detoxicating, eliminating dampness of invigorating blood circulation, for vaginal discharge diseases caused by wet poison stasis, symptoms include, profuse leukorrhea, color Huang taste is smelly, pudendal pruritus or vagina is scorching hot and pain, restlessness and thirst, fatigue and asthenia；Mucopurulent cervicitis (chlamydia trachomatis infection) is shown in above-mentioned patient.

Description

A kind of Chinese medicine composition and preparation method thereof for treating gynecological disease

Technical field

The present invention relates to a kind of Chinese medicine compositions and preparation method thereof for treating gynecological disease, belong to the technology neck of Chinese medicine Domain.

Background technique

Genital tract chlamydia trachomatis (CT) infection is sexually transmitted disease caused by the microorganism reported earliest (STD), although It there is no accurate number, but there are about 500,000,000 people Xin Fa STD in the World Health Organization (WHO) the report annual whole world, wherein there are about 90,000,000 Example (18%) is caused by CT infection.The total infection rate various regions report of CT is not quite similar in crowd, different crowd disease incidence difference Significantly, having data to show that healthy women generally investigates its disease incidence is only 1%~2%, and OPD of Obs & Gyn clients are 3%~9%, property Sick clinic clients are 18%~27%, and Female Sex Worker disease incidence is up to 33%.In addition, the age is obviously related to disease incidence.CT It is investigated in women population, British Women infection rate is 2%~12%；American Women's infection rate is 5%~15%.

Chlamydia is very harmful to women, especially young woman, because guild causes women cervicitis, basin on Chlamydia Chamber is scorching, salpingitis, ectopic pregnancy, can cause infertile, and serious person may cause cervical carcinoma, it is also possible to influence next-generation.CT Disease incidence do not occupy the first place of venereal disease, and have zooming trend, China is the largest infertility country now, Should more it be paid attention in China.

In recent years, China's choamydiae infection has zooming trend, and clinical treatment choamydiae infection is mainly adopted at present With antibiotic, but antibiotic is easy to develop drug resistance, therefore is difficult to eradicate, and is easy recurrence.Traditional Chinese medicine is in treatment gynecological infection Huge advantage is showed in property disease, however clinic has no the patent medicine for mucopurulent cervicitis (chlamydia trachomatis infection).

The present invention is the drug of research and development mainly for mucopurulent cervicitis (chlamydia trachomatis infection), has filled up clinic Lack the blank of the tcm product individually for chlamydia trachomatis infection.

Summary of the invention

The purpose of the present invention is to provide a kind of new Chinese medicine compositions, have the effect of clearing heat and detoxicating, eliminating dampness of invigorating blood circulation, and use The vaginal discharge diseases caused by wet poison stasis, symptoms include, profuse leukorrhea, color Huang taste is smelly, pudendal pruritus or vagina it is scorching hot and pain, restlessness and thirst, Fatigue and asthenia；Mucopurulent cervicitis (chlamydia trachomatis infection) is shown in above-mentioned patient.

It is a further object to provide the preparation methods of above-mentioned composition.

The present invention is achieved by the following technical solutions:

The present invention is made of the bulk pharmaceutical chemicals of following parts by weight:

Prescription one: 1-15 parts of radix gentianae, 1-15 parts of oldenlandia diffusa, 0.5-10 parts of the tuber of stemona, 0.1-7 parts of Rhizoma Chuanxiong, cortex dictamni 0.1-7 parts；

It is preferred that are as follows: 3-7 parts of radix gentianae, 3-7 parts of oldenlandia diffusa, 1-5 parts of the tuber of stemona, 1-4 parts of Rhizoma Chuanxiong, 1-4 parts of cortex dictamni；

More preferably: 5 parts of radix gentianae, 5 parts of oldenlandia diffusa, 3 parts of the tuber of stemona, 2 parts of Rhizoma Chuanxiong, 2 parts of cortex dictamni.

Prescription two: 1-15 parts of radix gentianae, 1-15 parts of oldenlandia diffusa, 0.5-10 parts of the tuber of stemona, 0.5-10 parts of the Chinese bulbul, the bark of ash 0.5-10 parts, 0.5-10 parts of radix paeoniae rubra, 0.1-7 parts of Rhizoma Chuanxiong, 0.1-7 parts of radix ampelopsis, 0.1-7 parts of cortex dictamni, 0.1-7 parts of Radix Glycyrrhizae.

It is preferred that are as follows: it is 3-7 parts of radix gentianae, 3-7 parts of oldenlandia diffusa, 1-5 parts of the tuber of stemona, 1-5 parts of the Chinese bulbul, 1-5 parts of the bark of ash, red 1-5 parts of Chinese herbaceous peony, 1-4 parts of Rhizoma Chuanxiong, 1-4 parts of radix ampelopsis, 1-4 parts of cortex dictamni, 1-4 parts of Radix Glycyrrhizae.

It is best preferably are as follows: 5 parts of radix gentianae, 5 parts of oldenlandia diffusa, 3 parts of the tuber of stemona, 3 parts of the Chinese bulbul, 3 parts of the bark of ash, 3 parts of radix paeoniae rubra, 2 parts of Rhizoma Chuanxiong, 2 parts of radix ampelopsis, 2 parts of cortex dictamni, 2 parts of Radix Glycyrrhizae.

In the present invention, radix gentianae, oldenlandia diffusa be monarch drug in a prescription, the tuber of stemona, the Chinese bulbul, cortex dictamni be ministerial drug, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis are adjutant, and Radix Glycyrrhizae is to make medicine.

The present invention, using above-mentioned active drug mutual cooperation prescription, reaches clearing heat and detoxicating using theory of traditional Chinese medical science as foundation, living The effect of blood eliminating dampness, can be used for treat morbid leucorrhea of gynaecology, in particular treatment mucopurulent cervicitis (chlamydia trachomatis infection) Effective prescription.

The mechanism of action of the Chinese medicine composition is as follows:

Radix gentianae: bitter, it is cold in nature.Return liver, gallbladder channel.With heat-clearing and damp-drying drug；The effect of purging the liver of pathogenic fire is calmed the frightened, can be used for treating damp and hot Jaundice, urine leaching pain, swelling of vulva pruritus vulvae, under humid tropics, the alliteration of liver and gallbladder excess fire is had a headache, and red eye, swell pain, deaf ear is swollen, hypochondriac pain mouth Hardship, pyreticosis convulsion etc..Pharmacological evaluation shows radix gentianae flooding agent in vitro to trichophyton gypseum star nocardia Equal dermatophytes have different degrees of inhibiting effect；Test tube method proves rough gentian decoction to Pseudomonas aeruginosa, proteus, typhoid fever bar Bacterium, shigella dysenteriae, staphylococcus aureus etc. have different degrees of inhibiting effect.

Oldenlandia diffusa: bitter sweet is trembled with fear, nontoxic.Return heart, liver, the spleen channel.With clearing heat and detoxicating, the effect of dampness removing, it to be used for lung heat It breathes with cough, abscess of throat, acute appendicitis, furuncle sore, venomous snake bite, puckery pain of heat gonorrhea, oedema, dysentery, enteritis, jaundice with damp-heat pathogen is good at and controls Treat a variety of cancerous swellings.Pharmacological evaluation shows that oldenlandia diffusa has antibacterial and anti-inflammation functions, and decoction tube dilution method, 1: 4 pair golden yellow Color staphylococcus, shigella flexneri, 1: 2 pair of typhoid bacillus, Pseudomonas aeruginosa etc. have inhibiting effect.

The tuber of stemona: sweet, bitter, tepor.It is distributed in lung channel.Have effects that moistening lung to arrest cough, desinsection and delousion, coughs for new chronic cough, tuberculosis, Pertussis, enterobiasis, body louse, tinea scabies etc..In vitro test shows: the tuber of stemona has resisting pathogenic microbes effect, tuber of stemona decoction and alcohol Preserved material is to pneumococcus, beta hemolytic streptococcus, meningococcus, staphylococcus aureus, staphylococcus albus, dysentery bar Bacterium, typhoid bacillus, paratyphosum Bacterium, Escherichia coli, proteus, corynebacterium diphtheriae, pneumobacillus, yersinia pestis, bacillus anthracis, Hay bacillus and comma bacillus, Bacillus tuberculosis etc. have different degrees of inhibiting effect Radix stemonae japonicae infusion in body There is certain inhibiting effect tuber of stemona decoction that can reduce Asia influenza A virus certain pathomycetes outside to cause a disease to mouse Power.

The Chinese bulbul: bitter, it is cold in nature.Return stomach, large intestine channel；It with clearing heat and detoxicating, removes heat from the blood and relieve diarrhea, the effect of removing dampness and destroying parasites.Medicine Reason is studies have shown that the Chinese bulbul has apparent antibacterial action to a variety of Grain-negatives and positive bacteria.The juicing pair of the Chinese bulbul Staphylococcus aureus, Pseudomonas aeruginosa have a stronger antibacterial action, decoction it is different according to its strain to the effect of shigella dysenteriae and Effect also difference, acts on shigella dysenteriae most strong, then successively weakens to the bacteriostasis of bacterium in Shu Shi, Fu Shi and Song, ethyl alcohol Immersion liquid in vitro has inhibiting effect to hay bacillus and staphylococcus aureus.The effective component of the Chinese bulbul is protoanemonin And Anemonin, all to staphylococcus aureus, streptococcus, corynebacterium diphtheriae, shigella dysenteriae, tubercle bacillus, Candida albicans etc. There is very strong inhibiting effect.

The bark of ash: bitter is trembled with fear.Return liver warp, gallbladder channel, large intestine channel.With heat-clearing and damp-drying drug, clear liver and improve vision, inducing astrigency stop dysentery, stop-band Effect.Pharmacological research shows bark of ash decoction to staphylococcus aureus, Escherichia coli, shigella flexneri, bacterium sonnei There is inhibiting effect.In addition, the bark of ash also has certain inhibiting effect, such as resisiting influenza virus, herpesviral to virus.

Radix paeoniae rubra: bitter, it is slightly cold.Return liver warp.There is the effect of clearing heat and cooling blood, removing blood stasis and acesodyne.At present to the research of radix paeoniae rubra, it is believed that Its principle active component is the monoterpene glycosides such as Paeoniflorin, albiflorin, oxypaeoniflorin, benzoylpaeoniflorin, peonin Compound, general name total paeony glycoside (TPG), wherein Paeoniflorin (PAE) accounts for larger proportion, has calm, analgesia spasmolysis, promoting blood circulationization The pharmacological actions such as the stasis of blood, platelet aggregation-against, antithrombotic.

Rhizoma Chuanxiong: acrid flavour, it is warm-natured；Return liver, gallbladder, the heart channel of Hang-Shaoyin.With blood-activating and qi-promoting, the effect of wind-expelling pain-stopping, irregular menstruation is cured mainly, Dysmenorrhea, menostasis, difficult labour, placenta retension, lochia abdominal pain, lump, heart pain in chest and hypochondrium, traumatic injury swelling and pain, headache and dizziness mesh Secretly, wind-cold-dampness arthralgia, extremity numbness, ulcer sores etc..Pharmacological evaluation shows Rhizoma Chuanxiong to large intestine, dysentery (sonnei), deformation, green Purulence, typhoid fever, paratyphosum Bacterium and comma bacillus etc. have inhibiting effect.Rhizoma Chuanxiong flooding agent (1: 3) is in vitro to certain pathogenic Dermatophyte also has inhibiting effect.

Radix ampelopsis: bitter sweet is pungent, cool.Return heart, liver, the spleen channel.Have effects that clearing heat and detoxicating, eliminating stagnation to stop pain, myogenic sore, it can For sore swollen toxin, scrofula, scald, wet sore, a kind of malaria, frightened epilepsy, bloody flux, intestines wind, anal fistula, leukorrhea, traumatic injury, traumatic hemorrhage Deng.Pharmacological evaluation shows radix ampelopsis flooding agent (1: 3) in vitro to Trichophyton concentricum, Microsporon audouini, abdomen stock The dermatophytes such as ditch and epidermophyton rubrum have different degrees of inhibiting effect.Flooding agent tube dilution method, 1: 15 concentration There is inhibiting effect to homocentricity trichophyta, microsporon audouini, epidermophyton inguinale etc..Root alcohol extract Methane Carboxylic Acid ester can Molten part causes mouse liver injury to have protective effect in carbon tetrachloride, and 300mg/kg significantly inhibits glutamic-pyruvic transaminase (SGPT), paddy The active raising of careless transaminase (SGOT).Clinical application can be used to treat surgery inflammation, and the peeling of radix ampelopsis root tuber is ground, takes 3 liang (dosage according to inflammation area add and subtract) with boiling water stirring it is agglomerating after, add 75~95% alcohol to be tuned into thick paste, be applied with affected part, daily 1 time, more for degree.For the initial stage of the acute infections such as furuncle, carbuncle, cellulitis, lymphnoditis, various inflammatory masses and masses, have significant Curative effect.

Cortex dictamni: bitter, it is salty, it is cold in nature.Returns spleen, lung, small intestine, stomach, bladder meridian.With heat-clearing and damp-drying drug；Dispelling wind and arresting itching；Removing toxic substances The effect of.Pharmacological research shows that cortex dictamni has antibacterial action, and the infusion of in vitro test 1: 4 is to a variety of pathomycetes such as violet Color trichophyta, Trichophyton concentricum, oidium schoenleinii have different degrees of inhibiting effect.

Radix Glycyrrhizae: mild-natured, sweet in flavor, return 12 warps.With invigorating the spleen and benefiting qi, clearing heat and detoxicating, expelling phlegm and arresting coughing, relieving spasm to stop pain is reconciled The effect of all medicines.Pharmacological research shows that Radix Glycyrrhizae has and adjusts body's immunity, and Radix Glycyrrhizae glucan can enhance immunity of organism function Can, there is activationa and proliferation effect to mouse spleen lymphocyte, shows to cause mitogen characteristic, shared synergistic effect with ConA. Radix Glycyrrhizae acids is mainly shown as the effect of enhancing macrophage phagocytic function and enhancing cellular immune function, but to humoral immunity function There can be inhibiting effect.Radix Glycyrrhizae also has antibacterial, antiviral, anti-inflammatory, antiallergic action, and liquorice flavonoids compound is to golden yellow Portugal Grape coccus, hay bacillus, saccharomycete, fungi, streptococcus etc. have inhibiting effect.Glycyrrhizin is to human immunity defective virus (AIDS virus, HIV), hepatitis virus, blister oral cavity virus, adenovirus type III, herpes simplex virus I-type, vaccinia virus Deng there is apparent inhibiting effect.Radix Glycyrrhizae has cortin sample anti-inflammatory effect, to mouse chemically auricle edema, abdominal cavity capillary Vasopermeability increases, swollen hyperplasia of rat granuloma, formaldehyde rat paw edema, Irish moss colloidality rat arthritis etc. have inhibition Effect.

Drug of the present invention can be prepared into any conventional preparation, including oral system using the conventional method of Chinese materia medica preparation Agent, external preparation, injection etc..Such as these bulk pharmaceutical chemicals can be ground into a powder uniformly mixed；It can be by the parts by weight Bulk pharmaceutical chemicals add the ethyl alcohol of water or various concentration to extract respectively, and extracting solution is concentrated and dried to obtain crude extract, or uses refining methd, example Such as, water extraction and alcohol precipitation method, organic solvent extractionprocess, column chromatography, carbon dioxide supercritical extraction method, steam distillation carry out essence Extract is made.

When stating drug in use, the drug to be equivalent to the weight proportion can both be used to clean respectively as raw material, It dries, crush, being mixed to get the particle for meeting formulation requirements granularity or powder is directly taken.It can also use described to be equivalent to The drug of weight proportion relation is that raw material adds pharmaceutic adjuvant after proper treatment, is made into various preparations as needed. During being prepared into preparation by above-mentioned raw materials medicine, above-mentioned raw materials medicine can be handled with the following method: by the weight The bulk pharmaceutical chemicals of part directly crush to obtain medicinal powder；Or respectively plus the ethyl alcohol of water or various concentration extract or using water extraction and alcohol precipitation method, Centrifugal process, extraction by steam distillation obtain extract；The adoptable specific behaviour when being extracted to above-mentioned effective medicinal ingredient Make and/or application method, either using the drug ingedient of each proportional quantities as raw material, extract respectively its it is effectively medicinal at The mode remixed after point, by the way of can also being extracted jointly again after by the mixing of each medicine material of described proportional quantities.It adopts Required ideal or optimal Extracting temperature when with different extraction means, equipment and extracting, extraction time, mentions solvent usage The actual conditions such as number are taken, then can be screened and find by test according to the actual situation.

In order to make each raw material of the drug preferably play drug effect, preferably the present invention is taken following preparation method:

1, prescription one the preparation method comprises the following steps:

Method one: radix gentianae, oldenlandia diffusa, the tuber of stemona, Rhizoma Chuanxiong, cortex dictamni water extract-alcohol precipitation obtain the traditional chinese medicine composition of the invention Active constituent；

Method two: oldenlandia diffusa, the tuber of stemona, cortex dictamni alcohol extracting, radix gentianae, Rhizoma Chuanxiong water mention, and obtain the traditional chinese medicine composition of the invention Active constituent；

Method three: radix gentianae, oldenlandia diffusa, the tuber of stemona, Rhizoma Chuanxiong, the shaggy-fruited dittany severe edema due to hypofunction of the spleen mentions or alcohol extracting, obtains Chinese traditional medicine composition of the present invention The active constituent of object；

Active constituent that any of the above-described method is prepared is added or is not added auxiliary material, and routinely technique is made pharmacy and takes up an official post A kind of what preparation.

2, prescription two the preparation method comprises the following steps:

Method one: radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae Water extract-alcohol precipitation obtains the active constituent of the traditional chinese medicine composition of the invention；

Method two: oldenlandia diffusa, the tuber of stemona, the bark of ash, cortex dictamni alcohol extracting, radix gentianae, the Chinese bulbul, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, Liquorice beverage mentions, and obtains the active constituent of the traditional chinese medicine composition of the invention.

Method three: radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae Water mentions or alcohol extracting, obtains the active constituent of the traditional chinese medicine composition of the invention.

In the preparation method of both the above prescription:

Abstract methods are as follows: add 6-10 times to measure water and extracts 1-3 times, it is 1-3 hours each；

It is preferred that are as follows: add 8 times of amount water to extract 2 times, every time 1.5 hours.

Alcohol extracting condition are as follows: add 6-10 times to measure 50-90% ethyl alcohol and extracts 1-3 times, it is 1-3 hours each；

It is preferred that are as follows: add 8 times of 70% ethyl alcohol of amount to extract 2 times, every time 2 hours.

Water extract-alcohol precipitation condition are as follows: add 6-10 times to measure water and extract 1-3 times, 1-3 hours each, filtration, filtrate is concentrated into 60 DEG C Relative density is 1.05-1.20 when survey, adds ethyl alcohol to make alcohol content up to 50-90%, stands；

It is preferred that are as follows: add 8 times of amount water to extract 2 times, 1.5 hours every time, filtration, relative density was when filtrate is concentrated into 60 DEG C of surveys 1.10-1.15 ethyl alcohol is added to make alcohol content up to 70%, stand.

The preparation method of the Chinese medicine composition of prescription two of the present invention is listed below:

1, radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 6-10 Amount water extracts 1-3 times again, 1-3 hours each, filtration, merging filtrate, and relative density is 1.05-1.20 when being concentrated into 60 DEG C of surveys, Add ethyl alcohol to make alcohol content up to 50-90%, stand, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, be prepared into To the active constituent of the traditional chinese medicine composition of the invention.

2, oldenlandia diffusa, the tuber of stemona, the bark of ash, cortex dictamni add 6-10 times to measure 50-90% ethyl alcohol and extract 1-3 times, and each 1-3 is small When, filtration, filtrate recycling ethanol to no alcohol taste obtains concentrate 1, spare；Radix gentianae, the Chinese bulbul, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, Radix Glycyrrhizae Add 6-10 times to measure water to extract 1-3 times, 1-3 hours each, filtration, merging filtrate, filtrate concentration obtains concentrate 2,1 He of concentrate Concentrate 2 merges, and continues to be concentrated into thick paste, the active constituent of the traditional chinese medicine composition of the invention is prepared.

3, radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add water or 50-90% ethyl alcohol extracts, and filtrate merges, and is concentrated into thick paste, the active constituent of the traditional chinese medicine composition of the invention is prepared.

Active constituent can also be prepared in the Chinese medicine composition of prescription one according to the method described above.

Method made above cannot be used for limiting the scope of the invention.

Active constituent made from a kind of preparation method of any of the above, which can directly be used as medicine, to be taken or is added in pharmacy and can connect Required preparation is produced in conventional fashion into the auxiliary material received.Common tablet (dispersible tablet, effervescent tablet, Orally disintegrating can such as be made Piece, lozenge, chewable tablets, effervescent tablet), capsule (hard capsule, soft capsule), granule, pill (pill), the solids such as powder The oral drugs of the liquid forms such as syrup, oral solution can also be made in the oral drugs of dosage form；Yin can also be made The external applications such as road piece, vagina effervescence, paste, gelling agent, ointment, cataplasm, emplastrum, liniment, lotion, suppository, plastics The external drug of dosage form.Therefore, in the pharmaceutical composition in addition to effective component, pharmaceutically acceptable can also be contained Auxiliary material.

Auxiliary material described here, can be different according to different preparations, such as solid in tablet, capsule, granule Common diluent, disintegrating agent, excipient, adhesive, lubricant, surfactant, filler etc. in body preparation；Syrup, Common surfactant in the liquid forms such as oral solution, preservative, stabilizer, corrigent, thickener, helps diluent Flow agent etc.；Common acceptable oil base, aqueous matrix, preservative, antioxygen in the external preparations form such as gelling agent, ointment Agent, moisturizer, skin penetration enhancer, surfactant etc..

Its common auxiliary material such as starch, lactose, dextrin, Icing Sugar, microcrystalline cellulose, mannitol, xylitol, polyethylene glycol, sulphur Sour calcium, calcium monohydrogen phosphate, calcium carbonate, modified starch, sorbierite, polyvinyl pyrrolidone, heavy magnesium carbonate, sodium carboxymethylcellulose, Hypromellose, methylcellulose, ethyl cellulose, carboxyrnethyl starch sodium, hydroxypropyl cellulose, PVP K30, white pottery Soil, pregelatinized starch, magnesium stearate, talcum powder, superfine silica gel powder, stevioside glycosides, glycine betaine, aspartame, glycyrrhizin, saccharin Sodium, citric acid, sodium bicarbonate, sodium carbonate, carragheen, agar, gelatin, sodium alginate, xanthan gum, guar gum, tragcanth, Arabic gum, Karaya Gum, stearic acid, glycerin monostearate, polyacrylamide, cross linked sodium polyacrylate, gathers locust bean gum Vinyl alcohol, carbomer, sorbic acid, potassium sorbate, ethyl hydroxy benzoate, Nipasol, methyl hydroxybenzoate, benzyl alcohol, nipalgin, sulphur willow Mercury, dimethyl sulfoxide, azone, triethanolamine, triethylamine, diethylamine, sodium hydroxide, glycerol, propylene glycol, isopropanol, BHT, BHA, lauryl sodium sulfate, Tweens, spans etc..

For preparation above-mentioned, the preparation method of the Chinese medicine composition of prescription two of the present invention is exemplified below:

Gelling agent is prepared with the following method:

The radix gentianaes of the parts by weight, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae adds 6-10 times to measure water extraction 1-3 times, and 1-3 hours each, filtration, merging filtrate, relative density is when being concentrated into 60 DEG C of surveys 1.05-1.20 adds ethyl alcohol to make alcohol content up to 50-90%, stands, and filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick Cream, it is spare；Gel-type vehicle, moisturizer are taken, is stirred evenly, appropriate solvent is added, stirs, makes sufficiently to be swollen, pH adjusting agent is added, Mix, above-mentioned thick paste, preservative is added, stirs evenly, be made gel to get.

Gelling agent is preferentially prepared with the following method:

The radix gentianaes of the parts by weight, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae adds 8 times of amount water to extract 2 times, and 1.5 hours every time, filtration, merging filtrate, relative density was 1.10- when being concentrated into 60 DEG C of surveys 1.15, add ethyl alcohol to make alcohol content up to 70%, stand, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, spare； Gel-type vehicle, moisturizer are taken, is stirred evenly, appropriate solvent is added, stirs, makes sufficiently to be swollen, pH adjusting agent is added, mixes, adds Enter above-mentioned thick paste, preservative, stir evenly, be made gel to get.

Ointment is prepared with the following method:

The radix gentianaes of the parts by weight, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae adds 6-10 times to measure water extraction 1-3 times, and 1-3 hours each, filtration, merging filtrate, relative density is when being concentrated into 60 DEG C of surveys 1.05-1.20 adds ethyl alcohol to make alcohol content up to 50-90%, stands, and filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick Cream, it is spare；Take stearic acid, glycerin monostearate, octadecyl alcolol, hexadecanol, light liquid petrolatum, MCT Oil (GTCC), Butylated Hydroxytoluene (BHT) in right amount, 85 DEG C of heating meltings, as oily phase；Separately take glycerol, propylene glycol, polyoxyethylene stearate (40) ester, sucrose stearate S-15, three potassium sorbates are appropriate, are added in thick paste and appropriate purified water and dissolve, and are heated to 85 DEG C, make For water phase；Water phase is slowly added into oily phase, 85 DEG C of heat preservation emulsifications, revolving speed 2000r/min continues to stir after emulsification 15 minutes It mixes and is cooled to 40 DEG C or so, stir 15 minutes, dispense to get ointment.

Ointment is preferentially prepared with the following method:

The radix gentianaes of the parts by weight, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae adds 8 times of amount water to extract 2 times, and 1.5 hours every time, filtration, merging filtrate, relative density was 1.10- when being concentrated into 60 DEG C of surveys 1.15, add ethyl alcohol to make alcohol content up to 70%, stand, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, spare； Take stearic acid, glycerin monostearate, octadecyl alcolol, hexadecanol, light liquid petrolatum, MCT Oil (GTCC), Butylated Hydroxytoluene (BHT) in right amount, 85 DEG C of heating meltings, as oily phase；Separately take glycerol, propylene glycol, polyoxyethylene stearate (40) ester, sugarcane Sugared stearate S-15, three potassium sorbates are appropriate, are added in thick paste and appropriate purified water and dissolve, 85 DEG C are heated to, as water phase；It will Water phase is slowly added into oily phase, 85 DEG C of heat preservation emulsifications, and revolving speed 2000r/min after emulsification 15 minutes, continues stirring and is cooled to 40 DEG C or so, it stirs 15 minutes, dispenses to get ointment.

Suppository is using following preparation method:

The radix gentianaes of the parts by weight, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae adds 6-10 times to measure water extraction 1-3 times, and 1-3 hours each, filtration, merging filtrate, relative density is when being concentrated into 60 DEG C of surveys 1.05-1.15 adds ethyl alcohol to make alcohol content up to 50-90%, stands, and filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick Cream, according to customary preparation methods be made suppository to get.

Suppository preferentially uses following preparation method:

The radix gentianaes of the parts by weight, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae adds 8 times of amount water to extract 2 times, and 1.5 hours every time, filtration, merging filtrate, relative density was 1.10- when being concentrated into 60 DEG C of surveys 1.15, add ethyl alcohol to make alcohol content up to 70%, stands, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, according to Suppository is made in suppository customary preparation methods.

Tablet is prepared with the following method:

The radix gentianaes of the parts by weight, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae adds 8 times of amount water to extract 2 times, and 1.5 hours every time, filtration, merging filtrate, relative density was 1.10- when being concentrated into 60 DEG C of surveys 1.15, add ethyl alcohol to make alcohol content up to 70%, stands, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, and it is dry, It is ground into fine powder, excipient, appropriate filler is added, mix, pelletize, it is dry, lubricant is added, mixes, tabletting, coating or not Coating is to get tablet.

Tablet is preferentially prepared with the following method:

Gelling agent, suppository, ointment, tablet can also be made according to above-mentioned preparation method in the Chinese medicine composition of prescription one Deng.

According to above content, under the premise of not departing from basic fundamental thought of the present invention, know according to the ordinary skill of this field Knowledge and customary means, it is clear that the modification, substitution and alteration of other diversified forms can also be made.

The specific embodiment of form by the following examples makees further specifically above content of the invention It is bright.But this should not be interpreted as to the scope of the above subject matter of the present invention is limited to the following embodiments.It is all above-mentioned interior based on the present invention Hold realized technology to all belong to the scope of the present invention.

Specific embodiment

Embodiment 1

It is radix gentianae 50g, oldenlandia diffusa 50g, tuber of stemona 30g, Chinese bulbul 30g, bark of ash 30g, radix paeoniae rubra 30g, Rhizoma Chuanxiong 20g, white Liana 20g, cortex dictamni 20g, Radix Glycyrrhizae 20g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 8 times of amounts Water extracts 2 times, and 1.5 hours every time, filtration, merging filtrate, relative density was 1.10-1.15 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol Make alcohol content up to 70%, stand, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, spare；Weigh appropriate card Wave nurse, glycerol, stir evenly, and sufficiently after wetting, add suitable quantity of water, stir, make sufficiently to be swollen, triethanolamine is added, mix, Above-mentioned thick paste, potassium sorbate is added, stirs evenly, be made gel to get.

Embodiment 2

It is radix gentianae 70g, oldenlandia diffusa 30g, tuber of stemona 50g, Chinese bulbul 10g, bark of ash 50g, radix paeoniae rubra 10g, Rhizoma Chuanxiong 40g, white Liana 10g, cortex dictamni 40g, Radix Glycyrrhizae 10g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 8 times of amounts Water extracts 2 times, and 1.5 hours every time, filtration, merging filtrate, relative density was 1.10-1.15 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol Make alcohol content up to 70%, stand, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, spare；Weigh hydroxypropyl first Glycerol, propylene glycol is added in appropriate cellulose, after stirring evenly, sufficiently soaking, adds suitable quantity of water, stirs, make sufficiently to be swollen, Be added triethanolamine, mix, above-mentioned thick paste, potassium sorbate is added, stirs evenly, be made gel to get.

Embodiment 3

It is radix gentianae 30g, oldenlandia diffusa 70g, tuber of stemona 10g, Chinese bulbul 50g, bark of ash 10g, radix paeoniae rubra 50g, Rhizoma Chuanxiong 10g, white Liana 40g, cortex dictamni 10g, Radix Glycyrrhizae 40g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 8 times of amounts Water extracts 2 times, and 1.5 hours every time, filtration, merging filtrate, relative density was 1.10-1.15 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol Make alcohol content up to 70%, stand, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, spare；Weigh appropriate card Wave nurse, glycerol, stir evenly, and sufficiently after wetting, add suitable quantity of water, stir, make sufficiently to be swollen, triethanolamine is added, mix, Above-mentioned thick paste, potassium sorbate is added, stirs evenly, gel is made to get gelling agent.

Embodiment 4

It is radix gentianae 150g, oldenlandia diffusa 10g, tuber of stemona 100g, Chinese bulbul 5g, bark of ash 100g, radix paeoniae rubra 5g, Rhizoma Chuanxiong 70g, white Liana 1g, cortex dictamni 70g, Radix Glycyrrhizae 1g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 8 times of amounts Water extracts 2 times, and 1.5 hours every time, filtration, merging filtrate, relative density was 1.10-1.15 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol Make alcohol content up to 70%, stand, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, spare；Weigh appropriate hydroxyl Third methylcellulose, glycerol, propylene glycol, stir evenly, and sufficiently after wetting, add suitable quantity of water, stir, make sufficiently to be swollen, are added Triethanolamine, mix, above-mentioned thick paste, potassium sorbate is added, stirs evenly, be made gel to get.

Embodiment 5

It is radix gentianae 10g, oldenlandia diffusa 150g, tuber of stemona 5g, Chinese bulbul 100g, bark of ash 5g, radix paeoniae rubra 100g, Rhizoma Chuanxiong 1g, white Liana 70g, cortex dictamni 1g, Radix Glycyrrhizae 70g

Embodiment 6

Radix gentianae 80g, oldenlandia diffusa 80g, tuber of stemona 50g, Chinese bulbul 50g, bark of ash 5g, radix paeoniae rubra 50g, Rhizoma Chuanxiong 30g, radix ampelopsis 30g, cortex dictamni 30g, Radix Glycyrrhizae 30g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 10 times of amounts Water extracts 1 time, and 3 hours every time, filtration, merging filtrate, relative density was 1.10-1.15 when being concentrated into 60 DEG C of surveys, and ethyl alcohol is added to make Alcohol content is stood up to 90%, and filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, and appropriate distilled water is added, and is made Obtain lotion.

Embodiment 7

It is radix gentianae 40g, oldenlandia diffusa 60g, tuber of stemona 20g, Chinese bulbul 40g, bark of ash 20g, radix paeoniae rubra 40g, Rhizoma Chuanxiong 30g, white Liana 15g, cortex dictamni 30g, Radix Glycyrrhizae 15g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 6 times of amounts Water extracts 3 times, and 1 hour every time, filtration, merging filtrate, relative density was 1.10-1.15 when being concentrated into 60 DEG C of surveys, and ethyl alcohol is added to make Alcohol content is stood up to 50%, and filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, dry, and dextrin is added, forms sediment Powder, appropriate mannitol mix, and pelletize, dry, and magnesium stearate is added, and mix, tabletting is to get tablet.

Embodiment 8

Oldenlandia diffusa, the tuber of stemona, the bark of ash, cortex dictamni add 8 times of 70% ethyl alcohol of amount to extract 2 times, 2 hours every time, filter, filtrate Ethyl alcohol is recycled to no alcohol taste, obtains concentrate 1, it is spare；Radix gentianae, the Chinese bulbul, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, Radix Glycyrrhizae add 8 times of amount water to mention It takes 2 times, 1.5 hours every time, filters, merging filtrate, filtrate concentration obtains concentrate 2, and concentrate 1 and concentrate 2 merge, continue It is concentrated into thick paste, it is spare；Appropriate carbomer, glycerol are weighed, is stirred evenly, sufficiently after wetting, suitable quantity of water is added, stirs, make Sufficiently swelling, be added triethylamine, mix, above-mentioned thick paste, potassium sorbate is added, stirs evenly, be made gel to get.

Embodiment 9

Radix gentianae 20g, oldenlandia diffusa 140g, tuber of stemona 8g, Chinese bulbul 80g, bark of ash 8g, radix paeoniae rubra 80g, Rhizoma Chuanxiong 5g, radix ampelopsis 60g, cortex dictamni 5g, Radix Glycyrrhizae 60g

Oldenlandia diffusa, the tuber of stemona, the bark of ash, cortex dictamni add 10 times of 50% ethyl alcohol of amount to extract 2 times, 1 hour every time, filter, filter Liquid recycles ethyl alcohol to no alcohol taste, obtains concentrate 1, spare；Radix gentianae, the Chinese bulbul, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, Radix Glycyrrhizae add 10 times of amount water It extracts 2 times, 1 hour every time, filters, merging filtrate, filtrate concentration obtains concentrate 2, and concentrate 1 and concentrate 2 merge, continue Be concentrated into thick paste, according to customary preparation methods be made suppository to get.

Embodiment 10

Oldenlandia diffusa, the tuber of stemona, the bark of ash, cortex dictamni add 6 times of 90% ethyl alcohol of amount to extract 3 times, 1.5 hours every time, filter, filter Liquid recycles ethyl alcohol to no alcohol taste, obtains concentrate 1, spare；Radix gentianae, the Chinese bulbul, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, Radix Glycyrrhizae add 6 times of amount water It extracts 3 times, 2 hours every time, filters, merging filtrate, filtrate concentration obtains concentrate 2, and concentrate 1 and concentrate 2 merge, continue It is concentrated into thick paste, it is spare；Appropriate carbomer, isopropanol are weighed, is stirred evenly, sufficiently after wetting, suitable quantity of water is added, stirs, Make sufficiently to be swollen, be added diethylamine, mix, above-mentioned thick paste, potassium sorbate is added, stirs evenly, be made gel to get.

Embodiment 11

Oldenlandia diffusa, the tuber of stemona, the bark of ash, cortex dictamni add 9 times of 60% ethyl alcohol of amount to extract 1 time, 3 hours every time, filter, filtrate Ethyl alcohol is recycled to no alcohol taste, obtains concentrate 1, it is spare；Radix gentianae, the Chinese bulbul, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, Radix Glycyrrhizae add 7 times of amount water to mention It takes 3 times, 1 hour every time, filters, merging filtrate, filtrate concentration obtains concentrate 2, and concentrate 1 and concentrate 2 merge, continue dense It is reduced to thick paste, it is spare；Appropriate carbomer, glycerol, hyaluronic acid are weighed, is stirred evenly, sufficiently after wetting, adds suitable quantity of water, Stirring, makes sufficiently to be swollen, and triethanolamine is added, and is uniformly mixed, above-mentioned thick paste, sorbic acid is added, stirs evenly, gel is made, Up to gelling agent.

Embodiment 12

It is radix gentianae 40g, oldenlandia diffusa 60g, tuber of stemona 20g, Chinese bulbul 40g, bark of ash 20g, radix paeoniae rubra 40g, Rhizoma Chuanxiong 10g, white Liana 30g, cortex dictamni 10g, Radix Glycyrrhizae 30g

Oldenlandia diffusa, the tuber of stemona, the bark of ash, cortex dictamni add 7 times of 80% ethyl alcohol of amount to extract 2 times, 3 hours every time, filter, filtrate Ethyl alcohol is recycled to no alcohol taste, obtains concentrate 1, it is spare；Radix gentianae, the Chinese bulbul, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, Radix Glycyrrhizae add 7 times of amount water to mention It takes 1 time, 3 hours every time, filters, merging filtrate, filtrate concentration obtains concentrate 2, and concentrate 1 and concentrate 2 merge, continue dense It is reduced to thick paste, it is dry, dextrin, mannitol, appropriate lactose is added, mixes, pelletizes, it is dry, magnesium stearate is added, mixes, tabletting, Coating or not to get tablet.

Embodiment 13

It is radix gentianae 60g, oldenlandia diffusa 40g, tuber of stemona 40g, Chinese bulbul 20g, bark of ash 40g, radix paeoniae rubra 20g, Rhizoma Chuanxiong 20g, white Liana 10g, cortex dictamni 20g, Radix Glycyrrhizae 10g

Oldenlandia diffusa, the tuber of stemona, the bark of ash, cortex dictamni add 8 times of 70% ethyl alcohol of amount to extract 2 times, 2 hours every time, filter, filtrate Ethyl alcohol is recycled to no alcohol taste, obtains concentrate 1, it is spare；Radix gentianae, the Chinese bulbul, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, Radix Glycyrrhizae add 8 times of amount water to mention It takes 2 times, 2 hours every time, filters, merging filtrate, filtrate concentration obtains concentrate 2, and concentrate 1 and concentrate 2 merge, continue dense It is reduced to thick paste, appropriate distilled water is added, lotion is made.

Embodiment 14

Oldenlandia diffusa, the tuber of stemona, the bark of ash, cortex dictamni add 8 times of 70% ethyl alcohol of amount to extract 2 times, 2 hours every time, filter, filtrate Ethyl alcohol is recycled to no alcohol taste, obtains concentrate 1, it is spare；Radix gentianae, the Chinese bulbul, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, Radix Glycyrrhizae add 8 times of amount water to mention It takes 2 times, 2 hours every time, filters, merging filtrate, filtrate concentration obtains concentrate 2, and concentrate 1 and concentrate 2 merge, continue dense It is reduced to thick paste, it is spare；Appropriate carbomer, glycerol are weighed, is stirred evenly, sufficiently after wetting, suitable quantity of water is added, stirs, make to fill Point swelling, be added triethanolamine, mix, above-mentioned thick paste, ethyl hydroxy benzoate is added, stirs evenly, be made gel to get.

Embodiment 15

It is radix gentianae 80g, oldenlandia diffusa 100g, tuber of stemona 60g, Chinese bulbul 90g, bark of ash 60g, radix paeoniae rubra 90g, Rhizoma Chuanxiong 50g, white Liana 60g, cortex dictamni 50g, Radix Glycyrrhizae 60g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 8 times of amounts Water extracts 2 times, and 1.5 hours every time, filtration, merging filtrate, relative density was 1.05-1.20 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol Make alcohol content up to 70%, stand, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, according to customary preparation methods Be made suppository to get.

Embodiment 16

It is radix gentianae 100g, oldenlandia diffusa 80g, tuber of stemona 90g, Chinese bulbul 60g, bark of ash 90g, radix paeoniae rubra 60g, Rhizoma Chuanxiong 60g, white Liana 50g, cortex dictamni 60g, Radix Glycyrrhizae 50g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 8 times of amounts Water extracts 2 times, and 2 hours every time, filtration, merging filtrate, relative density was 1.10-1.15 when being concentrated into 60 DEG C of surveys, and ethyl alcohol is added to make Alcohol content is stood up to 70%, and filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, spare；Weigh appropriate card wave Nurse, glycerol, stir evenly, and sufficiently after wetting, add suitable quantity of water, stir, make sufficiently to be swollen, triethanolamine is added, mix, add Enter above-mentioned thick paste, Nipasol, stir evenly, be made gel to get.

Embodiment 17

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 8 times of amounts Water extracts 2 times, and 1.5 hours every time, filtration, merging filtrate, filtrate was concentrated into thick paste, spare；Weigh appropriate carbomer, the third two Alcohol stirs evenly, and sufficiently after wetting, adds suitable quantity of water, stirs, make sufficiently to be swollen, triethanolamine is added, mix, in addition State thick paste, potassium sorbate, stir evenly, be made gel to get.

Embodiment 18

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 8 times of amounts 70% ethyl alcohol extracts 2 times, and 2 hours every time, filtration, filtrate recycling ethanol to no alcohol taste continued to be concentrated into thick paste, spare；It weighs Appropriate carbomer, glycerol, stir evenly, and sufficiently after wetting, add suitable quantity of water, stir, make sufficiently to be swollen, triethylamine is added, Mix, above-mentioned thick paste, sorbic acid is added, stirs evenly, be made gel to get.

Embodiment 19

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 8 times of amounts Water extracts 2 times, and 1.5 hours every time, filtration, merging filtrate, relative density was 1.10-1.15 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol Make alcohol content up to 70%, stand, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, spare；Take stearic acid, list Tristerin, octadecyl alcolol, hexadecanol, light liquid petrolatum, MCT Oil (GTCC), Butylated Hydroxytoluene (BHT) in right amount, 85 DEG C of heating meltings, as oily phase；Separately take glycerol, propylene glycol, polyoxyethylene stearate (40) ester, sucrose stearate Ester S-15, three potassium sorbates are appropriate, are added in thick paste and appropriate purified water and dissolve, 85 DEG C are heated to, as water phase；Slowly by water phase It being added in oily phase, 85 DEG C of heat preservation emulsifications, revolving speed 2000r/min after emulsification 15 minutes, continues stirring and is cooled to 40 DEG C or so, Stirring 15 minutes dispenses to get ointment.

Embodiment 20

Oldenlandia diffusa, the tuber of stemona, the bark of ash, cortex dictamni add 8 times of 70% ethyl alcohol of amount to extract 2 times, 2 hours every time, filter, filtrate Ethyl alcohol is recycled to no alcohol taste, obtains concentrate 1, it is spare；Radix gentianae, the Chinese bulbul, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, Radix Glycyrrhizae add 8 times of amount water to mention It takes 2 times, 1.5 hours every time, filters, merging filtrate, filtrate concentration obtains concentrate 2, and concentrate 1 and concentrate 2 merge, continue It is concentrated into thick paste, it is spare；Take stearic acid, glycerin monostearate, octadecyl alcolol, hexadecanol, light liquid petrolatum, medium chain fatty acid Triglycerides (GTCC), Butylated Hydroxytoluene (BHT) in right amount, 85 DEG C of heating meltings, as oily phase；Separately take glycerol, propylene glycol, stearic acid Poly- hydrocarbon oxygen (40) ester, sucrose stearate S-15, three potassium sorbates are appropriate, are added in thick paste and appropriate purified water and dissolve, are heated to 85 DEG C, as water phase；Water phase is slowly added into oily phase, 85 DEG C of heat preservation emulsifications, revolving speed 2000r/min, after emulsification 15 minutes, Continue stirring and be cooled to 40 DEG C or so, stir 15 minutes, dispenses to get ointment.

Embodiment 21

Radix gentianae, oldenlandia leather, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 8 times of amounts Water extracts 2 times, and 1.5 hours every time, filtration, merging filtrate, filtrate was concentrated into thick paste, emulsifiable paste is made according to customary preparation methods Agent to get.

Embodiment 22

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 8 times of amounts 70% ethyl alcohol extracts 2 times, and 2 hours every time, filtration, filtrate recycling ethanol to no alcohol taste continued to be concentrated into thick paste, spare；It takes hard Resin acid, glycerin monostearate, octadecyl alcolol, hexadecanol, light liquid petrolatum, MCT Oil (GTCC), fourth hydroxyl Toluene (BHT) in right amount, 85 DEG C of heating meltings, as oily phase；Separately take glycerol, propylene glycol, polyoxyethylene stearate (40) ester, sucrose hard Resin acid ester S-15, three potassium sorbates are appropriate, are added in thick paste and appropriate purified water and dissolve, 85 DEG C are heated to, as water phase；By water phase It is slowly added into oily phase, 85 DEG C of heat preservation emulsifications, revolving speed 2000r/min after emulsification 15 minutes, continues stirring and is cooled to 40 DEG C of left sides The right side is stirred 15 minutes, is dispensed to get ointment.

Embodiment 23

Radix gentianae 50g, oldenlandia diffusa 50g, tuber of stemona 30g, Rhizoma Chuanxiong 20g, cortex dictamni 20g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, Rhizoma Chuanxiong, cortex dictamni add 8 times of amount water to extract 2 times, 1.5 hours every time, filter, Merging filtrate, relative density is 1.05-1.20 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol to make alcohol content up to 70%, stands, filters, filter Liquid recycles ethyl alcohol to no alcohol taste, continues to be concentrated into thick paste, spare；Appropriate carbomer, glycerol are weighed, is stirred evenly, is sufficiently soaked Afterwards, suitable quantity of water is added, stirs, makes sufficiently to be swollen, triethanolamine is added, mixes, above-mentioned thick paste, potassium sorbate is added, is stirred Uniformly, be made gel to get.

Embodiment 24

Radix gentianae 70g, oldenlandia diffusa 30g, tuber of stemona 50g, Rhizoma Chuanxiong 40g, cortex dictamni 40g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, Rhizoma Chuanxiong, cortex dictamni add 8 times of amount water to extract 2 times, 1.5 hours every time, filter, Merging filtrate, relative density is 1.10-1.15 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol to make alcohol content up to 70%, stands, filters, filter Liquid recycles ethyl alcohol to no alcohol taste, continues to be concentrated into thick paste, spare；It is appropriate to weigh hydroxypropyl methylcellulose, glycerol, propylene glycol is added, After stirring evenly, sufficiently soaking, suitable quantity of water is added, stirs, makes sufficiently to be swollen, triethanolamine is added, is mixed, is added above-mentioned thick Cream, potassium sorbate, stir evenly, be made gel to get.

Embodiment 25

Radix gentianae 30g, oldenlandia diffusa 70g, tuber of stemona 10g, Rhizoma Chuanxiong 10g, cortex dictamni 10g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, Rhizoma Chuanxiong, cortex dictamni add 8 times of amount water to extract 2 times, 1.5 hours every time, filter, Merging filtrate, relative density is 1.10-1.15 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol to make alcohol content up to 70%, stands, filters, filter Liquid recycles ethyl alcohol to no alcohol taste, continues to be concentrated into thick paste, spare；Take stearic acid, glycerin monostearate, octadecyl alcolol, hexadecanol, Light liquid petrolatum, MCT Oil (GTCC), Butylated Hydroxytoluene (BHT) in right amount, 85 DEG C of heating meltings, as oil Phase；It separately takes glycerol, propylene glycol, polyoxyethylene stearate (40) ester, sucrose stearate S-15, three potassium sorbates appropriate, thick paste is added It is dissolved in appropriate purified water, 85 DEG C is heated to, as water phase；Water phase is slowly added into oily phase, 85 DEG C of heat preservation emulsifications, revolving speed For 2000r/min, after emulsification 15 minutes, continues stirring and be cooled to 40 DEG C or so, stir 15 minutes, dispense to get ointment.

Embodiment 26

Radix gentianae 150g, oldenlandia diffusa 10g, tuber of stemona 100g, Rhizoma Chuanxiong 70g, cortex dictamni 70g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, Rhizoma Chuanxiong, cortex dictamni add 8 times of amount water to extract 2 times, 1.5 hours every time, filter, Merging filtrate, relative density is 1.10-1.15 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol to make alcohol content up to 70%, stands, filters, filter Liquid recycles ethyl alcohol to no alcohol taste, continues to be concentrated into thick paste, dry, addition dextrin, starch, appropriate mannitol are mixed, pelletized, do It is dry, magnesium stearate is added, mixes, tabletting, is coated or is not coated to get tablet.

Embodiment 27

Oldenlandia diffusa, the tuber of stemona, cortex dictamni add 8 times of 70% ethyl alcohol of amount to extract 2 times, and 2 hours every time, filtration, filtrate was recycled Ethyl alcohol obtains concentrate 1 to no alcohol taste, spare；Radix gentianae, Rhizoma Chuanxiong add 8 times of amount water to extract 2 times, 1.5 hours every time, filter, merge Filtrate, filtrate concentration obtain concentrate 2, and concentrate 1 and concentrate 2 merge, continue to be concentrated into thick paste, spare；Weigh appropriate card wave Nurse, glycerol, stir evenly, and sufficiently after wetting, add suitable quantity of water, stir, make sufficiently to be swollen, triethanolamine is added, mix, add Enter above-mentioned thick paste, potassium sorbate, stir evenly, be made gel to get.

Embodiment 28

Radix gentianae 20g, oldenlandia diffusa 140g, tuber of stemona 8g, Rhizoma Chuanxiong 5g, cortex dictamni 5g

Oldenlandia diffusa, the tuber of stemona, cortex dictamni add 10 times of 50% ethyl alcohol of amount to extract 2 times, and 1 hour every time, filtration, filtrate was recycled Ethyl alcohol obtains concentrate 1 to no alcohol taste, spare；Radix gentianae, Rhizoma Chuanxiong add 10 times of amount water to extract 2 times, 1 hour every time, filter, merge Filtrate, filtrate concentration obtain concentrate 2, and concentrate 1 and concentrate 2 merge, continue to be concentrated into thick paste, according to customary preparation methods Be made suppository to get.

Embodiment 29

Radix gentianae 80g, oldenlandia diffusa 100g, tuber of stemona 60g, Rhizoma Chuanxiong 50g, cortex dictamni 50g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, Rhizoma Chuanxiong, cortex dictamni add 8 times of amount water to extract 2 times, 1.5 hours every time, filter, Merging filtrate, relative density is 1.10-1.15 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol to make alcohol content up to 70%, stands, filters, filter Liquid recycles ethyl alcohol to no alcohol taste, continues to be concentrated into thick paste, according to customary preparation methods be made suppository to get.

Embodiment 30

Radix gentianae 100g, oldenlandia diffusa 80g, tuber of stemona 90g, Rhizoma Chuanxiong 60g, cortex dictamni 60g

Radix gentianae, oldenlandia diffusa, the tuber of stemona, Rhizoma Chuanxiong, cortex dictamni add 8 times of amount water to extract 2 times, 2 hours every time, filter, close And filtrate, relative density is 1.10-1.15 when being concentrated into 60 DEG C of surveys, add ethyl alcohol to make alcohol content up to 70%, stands, filtration, and filtrate Ethyl alcohol is recycled to no alcohol taste, continues to be concentrated into thick paste, it is spare；Appropriate carbomer, glycerol are weighed, is stirred evenly, sufficiently after wetting, Suitable quantity of water is added, stirs, makes sufficiently to be swollen, triethanolamine is added, is mixed, above-mentioned thick paste, potassium sorbate is added, stirring is equal It is even, be made gel to get.

The present invention will be further illustrated by the following test examples the composition the utility model has the advantages that

The traditional chinese medicine composition of the invention is in vitro to the inhibiting effect research of mouse chlamydia trachomatis infection

1, material

1.1 cells and strain

Hela229 cell, mouse chlamydia trachomatis, measuring its TCID50 according to iodine solution decoration method is 10-5/0.2ml.

1.2 reagent

DMEM, fetal calf serum, mycillin, 0.25% trypsase, gentamicin, cycloheximide, PBS, iodine grain, iodate Potassium, methanol.

1.3 test medicine

Medicine group 1 is made according to the embodiment of the present invention 1；

Medicine group 2 is made according to the embodiment of the present invention 23；

It is made into the mother liquor of 40mg/ml before test with high purity water respectively, it is spare after filtration sterilization.

Positive control drug: Doxycycline Hyclate, dosage are 32 μ g/ml.

1.4 key instrument equipment

Inverted microscope, CO₂Incubator, superclean bench, Biohazard Safety Equipment, centrifuge, weighs day at cell counter It is flat.

1.5 solution are prepared

1.5.1 cell growth medium: DMEM 45ml, FBS 5ml, mycillin, which is added, makes final concentration of 100 μ g/ml.

1.5.2 mouse Chlamydia development culture medium: 10 μ g/ml of gentamicin final concentration is added in DMEM 45ml, FBS 5ml, 2 μ g/ml of cycloheximide final concentration.

5% iodine solution: iodine grain 5g, potassium iodide 5g, methanol 50ml, distilled water 50ml.

2, method

2.1 toxicity trials (mtt assay)

After mother liquid medicine is done 1: 2~1: 256 doubling dilutions with culture solution, it is added to the Hela229 cell for having grown up to single layer In culture plate, 100 holes μ L/, each dilution medical fluid does 4 multiple holes, while setting normal cell controls.Culture plate is set 37 DEG C, 5%CO₂It is cultivated in incubator, 10 μ l 0.5%MTT solution are added in every hole after 48h, continue to cultivate 4h, supernatant are carefully sucked out, often 100 μ lDMSO are added in hole, and after shaking dissolved particles, OD value is measured under 570nm, calculate 50% cell toxicant by Reed-Muench method Property concentration (TC₅₀) and maximal non-toxic concentration (TC₀)。

The 2.2 cell monolayer preparation for infection

Take 75cm²The good Hela229 cell of growth conditions in culture bottle, after 0.25% pancreatin 3ml digestion, with containing The DMEM 3ml of 10%FBS is neutralized, and is centrifuged under 800rmp, 6min room temperature, and supernatant is abandoned, and the DMEM containing 10%FBS is added, and piping and druming is adjusted It after whole cell concentration, is inoculated in 2 24 orifice plates and (is pre-placed 13mm circle slide in hole), in 37 DEG C, 5%CO₂Incubator Middle culture, until cell adherent growth can carry out next step operation in 90% fusion.

The inoculation of 2.3 mouse chlamydia trachomatises and dosing

Cell culture medium in 24 orifice plates is discarded, is washed 2-3 times with PBS, mouse Chlamydia is diluted to the DMEM without FBS 10-2.5,0.2ml infection cell is added in every hole, and sets 4 negative control holes, and the DMEM for being free of mouse Chlamydia is added.It will inoculation 24 orifice plates afterwards were placed in a centrifuge at normal temperature, with 1500rpm centrifugation 1 hour.Centrifugation is placed on 37 DEG C, 5%CO2 incubator In 1 hour.In Biohazard Safety Equipment, DMEM in hole is sucked out, the mouse Chlamydia development culture containing relative medicine concentration is added Base is placed in 37 DEG C, 5%CO₂It is cultivated 48-72 hours in incubator.

2.4 dyeing and result observation

24 orifice plates are taken out, supernatant is abandoned, after methanol fixes 10min, slide is taken out, is dyed with 5% iodine solution, in microscope Under see in infected Hela cell and there is the inclusion body of brown to be formed.Observe the infection cell that inclusion body is formed in each hole Percentage, and score.

Cytopathy is judged by 6 grade standards:

: cell growth is normal, and no lesion occurs；

±: cells inclusions lesion is less than the 10% of entire single layer；

+: cells inclusions lesion accounts for about 25% or less entire cell monolayer；

++: cells inclusions lesion accounts for about 50% or less entire cell monolayer；

+++: cells inclusions lesion accounts for about 75% or less entire cell monolayer；

++++: cells inclusions lesion accounts for about 75% or more of entire cell monolayer.

50% inhibition concentration (IC50) and therapeutic index (TI), TI=TC50/IC50 are calculated by Reed-Muench.

3, result

Toxic effect of 3.1 the traditional chinese medicine composition of the invention to Hela229 cell

TC of the medicine group 1 to Hela229 cell₀For 0.63mgmL^-1, TC₅₀For 4.45mgmL^-1, medicine group 2 is right The TC of Hela229 cell₀For 2.50mgmL^-1, TC₅₀For 7.52mgmL^-1, specifically it is shown in Table 1.

Toxic effect of 1 the traditional chinese medicine composition of the invention of table to Hela229 cell

Drug concentration unit: mgmL^-1

3.2 the traditional chinese medicine composition of the invention are in vitro to the inhibiting effect of mouse chlamydia trachomatis

Medicine group 1 is in 0.63mgmL^-1And 0.32mgmL^-1When concentration, in vitro to the packet of mouse chlamydia trachomatis formation Contain body to significantly inhibit, IC₅₀For 0.22mgmL^-1, TI 20.23；Medicine group 2 is in 1.25mgmL^-1Concentration When, the inclusion body formed in vitro to mouse chlamydia trachomatis significantly inhibits, IC₅₀For 0.98mgmL^-1, TI It is 15.44.

2 the traditional chinese medicine composition of the invention of table inhibits the lesion situation of mouse chlamydia trachomatis in vitro

Drug concentration unit: mgmL^-1

The external anti-mouse chlamydia trachomatis effect of 3 the traditional chinese medicine composition of the invention of table

IC₅₀: mgmL^-1

Embodiment of the present invention 2-22,23-30 has carried out similar test according to the method described above, as a result with the above results phase Seemingly, it all can be shown that the traditional chinese medicine composition of the invention there is apparent inhibition to make the inclusion body that mouse chlamydia trachomatis is formed in vitro With.

4, conclusion

To sum up, the traditional chinese medicine composition of the invention can effectively inhibit chlamydia trachomatis infection, prompt the traditional chinese medicine composition of the invention can To treat morbid leucorrhea of gynaecology symptoms include profuse leukorrhea, color Huang taste is smelly, pudendal pruritus or vagina is scorching hot and pain, restlessness and thirst, and body is tired It is out of strength, it especially can effectively treat mucopurulent cervicitis (chlamydia trachomatis infection).

Claims

1. a kind of Chinese medicine composition for treating gynecological disease, which is characterized in that it is made of the bulk pharmaceutical chemicals of following parts by weight: dragon 3-7 parts of gallbladder grass, 3-7 parts of oldenlandia diffusa, 1-5 parts of the tuber of stemona, 1-4 parts of Rhizoma Chuanxiong, 1-4 parts of cortex dictamni.

2. a kind of Chinese medicine composition for treating gynecological disease, which is characterized in that it is made of the bulk pharmaceutical chemicals of following parts by weight: dragon 3-7 parts of gallbladder grass, 3-7 parts of oldenlandia diffusa, 1-5 parts of the tuber of stemona, 1-5 parts of the Chinese bulbul, 1-5 parts of the bark of ash, 1-5 parts of radix paeoniae rubra, Rhizoma Chuanxiong 1-4 Part, 1-4 parts of radix ampelopsis, 1-4 parts of cortex dictamni, 1-4 parts of Radix Glycyrrhizae.

3. the preparation method of Chinese medicine composition as claimed in claim 1 or 2, which is characterized in that it is prepared:

Above-mentioned raw materials medicine is directly crushed and obtains medicinal powder；Or the ethyl alcohol of water or various concentration is added to extract, extracting solution concentration is dry It is dry to obtain crude extract；Or it further carries out being refining to obtain extract using water extraction and alcohol precipitation method, column chromatography, steam distillation；Medicine Material powder or the crude extract being prepared or extract are the active constituent of the Chinese medicine composition, and the active constituent is routinely Any preparation is made in pharmacy in technique.

4. the preparation method of Chinese medicine composition described in claim 1, which is characterized in that it is prepared:

Method one: radix gentianae, oldenlandia diffusa, the tuber of stemona, Rhizoma Chuanxiong, cortex dictamni water extract-alcohol precipitation obtain the activity of the Chinese medicine composition Ingredient；

Method two: oldenlandia diffusa, the tuber of stemona, cortex dictamni alcohol extracting, radix gentianae, Rhizoma Chuanxiong water mention, and obtain the activity of the Chinese medicine composition Ingredient；

Method three: radix gentianae, oldenlandia diffusa, the tuber of stemona, Rhizoma Chuanxiong, the shaggy-fruited dittany severe edema due to hypofunction of the spleen mentions or alcohol extracting, obtains the work of the Chinese medicine composition Property ingredient；

Active constituent that any of the above-described method is prepared is added or auxiliary material is not added routinely technique is made any one in pharmacy Kind preparation.

5. the preparation method of Chinese medicine composition described in claim 2, which is characterized in that it is prepared:

Method one: radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, liquorice beverage mention Alcohol precipitation obtains the active constituent of the Chinese medicine composition；

Method two: oldenlandia diffusa, the tuber of stemona, the bark of ash, cortex dictamni alcohol extracting, radix gentianae, the Chinese bulbul, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, Radix Glycyrrhizae Water mentions, and obtains the active constituent of the Chinese medicine composition；

Method three: radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, liquorice beverage mention Or alcohol extracting, obtain the active constituent of the Chinese medicine composition；

6. preparation method according to claim 4 or 5, which is characterized in that in this method:

Water extract-alcohol precipitation condition are as follows: add 6-10 times to measure water and extracts 1-3 times, 1-3 hours each, filtration, when filtrate is concentrated into 60 DEG C of surveys Relative density is 1.10-1.20, adds ethyl alcohol to make alcohol content up to 50-90%, stands.

7. preparation method according to claim 4 or 5, which is characterized in that the preparation refers to external preparation.

8. preparation method according to claim 7, which is characterized in that the external preparation refers to vaginal tablets, suppository, washes Agent, gelling agent, ointment, cream.

9. preparation method according to claim 8, which is characterized in that gelling agent is prepared:

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 6-10 times to measure water It extracts 1-3 times, 1-3 hours each, filtration, merging filtrate, relative density is 1.05-1.20 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol Make alcohol content up to 50-90%, stand, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, spare；Take gel base Matter, moisturizer, stir evenly, and appropriate solvent is added, and stirring makes sufficiently to be swollen, and pH adjusting agent is added, and mixes, and are added above-mentioned thick Cream, preservative, stir evenly, be made gel to get.

10. preparation method according to claim 8, which is characterized in that suppository is prepared:

Radix gentianae, oldenlandia diffusa, the tuber of stemona, the Chinese bulbul, the bark of ash, radix paeoniae rubra, Rhizoma Chuanxiong, radix ampelopsis, cortex dictamni, Radix Glycyrrhizae add 6-10 times to measure water It extracts 1-3 times, 1-3 hours each, filtration, merging filtrate, relative density is 1.05-1.15 when being concentrated into 60 DEG C of surveys, adds ethyl alcohol Make alcohol content up to 50-90%, stand, filtration, filtrate recycling ethanol to no alcohol taste continues to be concentrated into thick paste, according to conventional preparation Method be made suppository to get.

11. the application that Chinese medicine composition of any of claims 1 or 2 is used to prepare treatment mucopurulent cervicitis drug.