CN105613736A - Composite probiotic beverage prepared by utilizing pawpaw extract - Google Patents
Composite probiotic beverage prepared by utilizing pawpaw extract Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/732—Chaenomeles, e.g. flowering quince
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8994—Coix (Job's tears)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
Abstract
The invention belongs to the technical field of food processing and discloses a composite probiotic beverage prepared by utilizing a pawpaw extract. The preparation method of the composite probiotic beverage comprises the steps of 1 preparation of the pawpaw extract, 2 preparation of a seabuckthorn extract, 3 preparation of a compound A, 4 preparation of a compound B and 5 preparation of the beverage. The composite probiotic beverage is unique in preparation process, can effectively control blood sugar and blood fat and is an effective dietary therapy mode.
Description
Technical field
The invention belongs to food processing technology field, more particularly to a kind of compound probiotic beverage utilizing Fructus Chaenomelis extract to prepare.
Background technology
Fructus Chaenomelis is the dry almost ripe fruit of rosaceous plant chaenomeles lagenaria, and sour in the mouth, temperature return liver, spleen channel. Along with going deep into of pharmacology of Chinese materia medica research, it has been found that Fructus Chaenomelis contains multiple pharmacological component, including triterpenes, organic acid, flavonoid and vitamin C etc. Main triterpenes components is pentacyclic triterpenoid, including oleanolic acid (oleanolicacid) and ursolic acid (ursolicacid) etc.
Fructus Hippophae is a kind of fallen leaves property shrub, and its characteristic is drought-enduring, anti-blown sand, it is possible to survives on wetland with saline-alkaline, is therefore widely used in water and soil conservation. Northwest China plants Fructus Hippophae in a large number, for desert afforestation. In sea buckthorn fruit, Vitamin C content is high, have the laudatory title of the king of vitamin C. Fructus Hippophae is the general designation of plant and its fruit. Plant Fructus Hippophae is Elaeangnaceae Hippophae, is a kind of fallen leaves property shrub. Domestic it is distributed in the ground such as North China, northwest, southwest. Fructus Hippophae is medicinal and edible plant. The root of Fructus Hippophae, stem, leaf, flower, really, particularly sea buckthorn fruit contains abundant nutrient substance and bioactive substance, it is possible to be widely used in many fields of the national economy such as food, medicine, light industry, space flight, agriculture and animal husbandry fishery. Fructus Hippophae can reduce cholesterol and blood glucose, and allevating angina pectoris shows effect, and also has the effect preventing and treating coronary atherosclerotic heart disease.
Probiotic bacteria beverage in the market is varied, and its emphasis also has difference, for instance the high calcium milk for calcium deficiency crowd and the probiotics milk for constipation crowd etc. diabetes are by inherited genetic factors, immunologic function disorder, microorganism infection and toxin thereof, free radical toxin, the various virulence factor of Nervous and Mental Factors etc. acts on body and causes hypoinsulinism, insulin resistant etc. and the sugar that causes, protein, fat, a series of metabolism disorder syndromes such as power and water Xie Zhi, clinically with hyperglycemia for main feature, model case may occur in which polyuria, polydipsia, polyphagia, the performance such as become thin, i.e. " three-many-one-little " symptom, diabetes (blood glucose) are once control bad to cause complication, cause kidney, eye, the exhaustion pathological changes at the positions such as foot, and cannot cure. health is had bigger toxic and side effects by Long-term taking medicine, and affects the function of stomach, liver, kidney. blood glucose can be controlled if need not take medicine, and normal diet can be participated in, then diabetics will palliate the agonizing sufferings significantly, and quality of life is greatly improved.
Summary of the invention
In order to overcome the deficiencies in the prior art, the invention provides a kind of compound probiotic beverage utilizing Fructus Chaenomelis extract to prepare, its preparation technology is unique, can efficiently control blood glucose and blood fat.
For achieving the above object, the technical solution used in the present invention is as follows:
A kind of compound probiotic beverage utilizing Fructus Chaenomelis extract to prepare, it is prepared as follows and obtains: 1) prepare Fructus Chaenomelis extract; 2) Fructus Hippophae extract is prepared; 3) complex A is prepared; 4) complex B is prepared; 5) beverage is prepared.
Specifically, it is prepared as follows and obtains: 1) prepare Fructus Chaenomelis extract: cuts seed open after being cleaned by Fructus Chaenomelis, is cut into fragment, air-dry, it is ground into powder, is then placed in the ethanol that volume fraction is 70% and soaks 30 minutes, not being as the criterion, decompression rotary evaporation removes ethanol; Ultra-vioket radiation 15min subsequently, then add the soak with ethanol 30min that volume fraction is 85%, the then centrifugal supernatant of collecting of 1000 turns/min, the extractum that supernatant concentration becomes density be 1.2g/ml, 80 DEG C are dried, are ground into powder, to obtain final product;
2) prepare Fructus Hippophae extract: Fructus Hippophae high-speed tissue mashing machine pulled an oar, then add water boil 20min, remove slag by three layers filtered through gauze, collect filtrate and get final product; The mass ratio of described Fructus Hippophae and water is 1:3;
3) complex A is prepared: by weight ratio mix homogeneously according to 2:1 of Fructus Lycii and Radix Arctii, add the soak by water 1 hour of 5 times of weight, collect by filtration filtrate, the pH adjusting filtrate with the hydrochloric acid solution of 1mol/L is 6.5, then filtrate is condensed into the extractum that density is 1.1g/ml, 80 DEG C dried, is ground into powder and get final product;
4) prepare complex B: by Semen oryzae sativae and Semen Coicis according to the weight ratio mix homogeneously of 2:1, be then ground to Powdered, cross 200 mesh sieves and get final product;
5) beverage is prepared: put in milk by Fructus Chaenomelis extract, Fructus Hippophae extract, complex A, complex B and stabilizer, mix homogeneously, it is subsequently placed in 95 DEG C to sterilize 160 seconds, cool after 32 DEG C, add Lactobacillus bulgaricus, ferment 12 hours, terminate fermentation, finally cool 4 DEG C, package encapsulation and get final product; Wherein, the concentration of Lactobacillus bulgaricus is 5 �� 108Individual/ml, addition is identical with stabilizer weight.
The mass ratio of described Fructus Chaenomelis extract, Fructus Hippophae extract, complex A, complex B, stabilizer and milk is 3-5:5-7:1-2:3-5:1-2:100-150;
Described stabilizer is obtained by mixing according to the mass ratio of 5:1:3 by sodium hexameta phosphate, xanthan gum and sodium alginate.
The beneficial effect that the present invention obtains specifically includes that
Fructus Chaenomelis extract prepared by the present invention contains the materials such as oleanolic acid, ursolic acid and vitamin C, it is possible to be effectively reduced blood glucose and blood fat, improves physical function; Fructus Hippophae extract prepared by the present invention contains abundant vitamin and Fructus Hippophae flavone, possesses the effects such as good reducing blood sugar and blood fat; The present invention, by adding multiple rare Chinese medicine extract, improves the medical value of beverage; The present invention, containing profitable probliotics, promotes that intestinal absorption is effective; Beverage prepared by the present invention can effectively prevent and treat hyperglycemia hyperlipidemia, it is not necessary to take medicine, it is to avoid the toxic and side effects that drug administration brings, and supplemented with energy, kills two birds with one stone. Present invention process is unique, and different raw materials adopts different processing modes, it is to avoid the nutritional labeling waste that single processing mode causes, can large-scale industrial production, prospect is better.
Detailed description of the invention
According to following embodiment, it is possible to be more fully understood that the present invention. But, as it will be easily appreciated by one skilled in the art that the concrete material proportion described by embodiment, process conditions and result thereof are merely to illustrate the present invention, and should without the present invention described in detail in restriction claims.
Embodiment 1
A kind of compound probiotic beverage utilizing Fructus Chaenomelis extract to prepare, it is prepared as follows and obtains:
1) Fructus Chaenomelis extract is prepared: cut seed open after being cleaned by Fructus Chaenomelis, be cut into fragment, air-dry, it is ground into powder, is then placed in the ethanol that volume fraction is 70% and soaks 30 minutes, be not as the criterion, decompression rotary evaporation removes ethanol; Ultra-vioket radiation 15min subsequently, then add the soak with ethanol 30min that volume fraction is 85%, the then centrifugal supernatant of collecting of 1000 turns/min, the extractum that supernatant concentration becomes density be 1.2g/ml, 80 DEG C are dried, are ground into powder, to obtain final product;
2) prepare Fructus Hippophae extract: Fructus Hippophae high-speed tissue mashing machine pulled an oar, then add water boil 20min, remove slag by three layers filtered through gauze, collect filtrate and get final product; The mass ratio of described Fructus Hippophae and water is 1:3;
3) complex A is prepared: by weight ratio mix homogeneously according to 2:1 of Fructus Lycii and Radix Arctii, add the soak by water 1 hour of 5 times of weight, collect by filtration filtrate, the pH adjusting filtrate with the hydrochloric acid solution of 1mol/L is 6.5, then filtrate is condensed into the extractum that density is 1.1g/ml, 80 DEG C dried, is ground into powder and get final product;
4) prepare complex B: by Semen oryzae sativae and Semen Coicis according to the weight ratio mix homogeneously of 2:1, be then ground to Powdered, cross 200 mesh sieves and get final product;
5) beverage is prepared: put in milk by Fructus Chaenomelis extract, Fructus Hippophae extract, complex A, complex B and stabilizer, mix homogeneously, it is subsequently placed in 95 DEG C to sterilize 160 seconds, cool after 32 DEG C, add Lactobacillus bulgaricus, ferment 12 hours, terminate fermentation, finally cool 4 DEG C, package encapsulation and get final product; Wherein, the concentration of Lactobacillus bulgaricus is 5 �� 108Individual/ml, addition is identical with stabilizer weight.
The mass ratio of described Fructus Chaenomelis extract, Fructus Hippophae extract, complex A, complex B, stabilizer and milk is 3:5:1:3:1:100;
Described stabilizer is obtained by mixing according to the mass ratio of 5:1:3 by sodium hexameta phosphate, xanthan gum and sodium alginate.
Embodiment 2
A kind of compound probiotic beverage utilizing Fructus Chaenomelis extract to prepare, it is prepared as follows and obtains:
1) Fructus Chaenomelis extract is prepared: cut seed open after being cleaned by Fructus Chaenomelis, be cut into fragment, air-dry, it is ground into powder, is then placed in the ethanol that volume fraction is 70% and soaks 30 minutes, be not as the criterion, decompression rotary evaporation removes ethanol; Ultra-vioket radiation 15min subsequently, then add the soak with ethanol 30min that volume fraction is 85%, the then centrifugal supernatant of collecting of 1000 turns/min, the extractum that supernatant concentration becomes density be 1.2g/ml, 80 DEG C are dried, are ground into powder, to obtain final product;
2) prepare Fructus Hippophae extract: Fructus Hippophae high-speed tissue mashing machine pulled an oar, then add water boil 20min, remove slag by three layers filtered through gauze, collect filtrate and get final product; The mass ratio of described Fructus Hippophae and water is 1:3;
3) complex A is prepared: by weight ratio mix homogeneously according to 2:1 of Fructus Lycii and Radix Arctii, add the soak by water 1 hour of 5 times of weight, collect by filtration filtrate, the pH adjusting filtrate with the hydrochloric acid solution of 1mol/L is 6.5, then filtrate is condensed into the extractum that density is 1.1g/ml, 80 DEG C dried, is ground into powder and get final product;
4) prepare complex B: by Semen oryzae sativae and Semen Coicis according to the weight ratio mix homogeneously of 2:1, be then ground to Powdered, cross 200 mesh sieves and get final product;
5) beverage is prepared: put in milk by Fructus Chaenomelis extract, Fructus Hippophae extract, complex A, complex B and stabilizer, mix homogeneously, it is subsequently placed in 95 DEG C to sterilize 160 seconds, cool after 32 DEG C, add Lactobacillus bulgaricus, ferment 12 hours, terminate fermentation, finally cool 4 DEG C, package encapsulation and get final product.
The mass ratio of described Fructus Chaenomelis extract, Fructus Hippophae extract, complex A, complex B, stabilizer and milk is 5:7:2:5:2:150;
Described stabilizer is obtained by mixing according to the mass ratio of 5:1:3 by sodium hexameta phosphate, xanthan gum and sodium alginate.
Embodiment 3
Experiment of Zoology
Experimental animal selects: choosing the Wistar male rat 50 of health, (200 �� 10) g, Shandong University's Experimental Animal Center provides. Rat adapts to feed after 3 days at Animal House, randomly draws 10 and is only used as Normal group and feeds with common maintenance feedstuff. High glucose and high fat feed formula: rat normal feedstuff 68%, sucrose 10%, yolk powder 9%, Adeps Sus domestica 8%, cholesterol 1%, cholate 0.2%, surplus is other materials.
Embodiment: all the other are 40 only with high glucose and high fat forage feed 4 weeks. After 4 weeks, rat is after fasting 18h, streptozotocin (STZ) intravenous injection 20mg/Kg, and every two days once, and after using twice, the high person of blood pressure and blood lipoid is modeling success. Choose the successful rat of modeling, be randomly divided into 3 groups, often group 10, namely model group (not using any medicine), of the present invention group and matched group: all use gliclazide (10mg/kg), method of application is oral, and sooner or later respectively once, before feed, half an hour takes; The beverage 50ml of of the present invention group of extra daily embodiment 1 preparation. Detect after taking continuously 10 days.
Statistical analysis: data information adopts x �� s to represent, uses SPSS17.0 statistical software to carry out data analysis, compares employing variance analysis between many groups, compares employing t inspection between two groups. P < 0.05 is statistically significant.
Therapeutic effect: have detected the biochemical indicators such as fasting glucose (FBG), T-CHOL (TC), triglyceride (TG), unit is mmol/L, and concrete outcome is in Table 1.
Table 1
Conclusion: by upper table it can be seen that the beverage of the embodiment of the present invention 1 preparation can coordinate Western medicine effectively, significantly reduce FBG, TC, TG.
Embodiment 4
Clinical model case:
Grandson, man 56 years old, people from Luozhuang District, fasting glucose 7.5mmol/L, two hours blood glucose 8.9mmol/L after the meal, the beverage of the edible embodiment of the present invention 2 preparation one month, every day is sooner or later respectively once, each 250ml, fasting glucose is down to 6.1mmol/L, and two hours blood glucoses are down to 7.8mmol/L after the meal, adheres to the beverage of the edible present invention, follow up a case by regular visits to half a year, without rebound phenomena.
Liu so-and-so, man 61 years old, people from Hedong District, suffer from diabetes for many years, taking metformin hydrochloride to treat, fasting glucose controls at about 6.6mmol/L always, controls effect general, the beverage of the edible embodiment of the present invention 2 preparation is after one month, fasting glucose is down to 5.9mmol/L, and then Western medicine reduces by half, and continues the beverage of the edible present invention, follow up a case by regular visits to 6 months, without rebound phenomena.
The present invention has been carried out detailed description by above-mentioned employing specific embodiment; but not limiting the scope of the invention; one of ordinary skill in the art should be understood that; on the basis of technical scheme, those skilled in the art need not pay various amendments or deformation that creative work can make still within protection scope of the present invention.
Claims (4)
1. utilizing a compound probiotic beverage prepared by Fructus Chaenomelis extract, it is prepared as follows and obtains: 1) prepare Fructus Chaenomelis extract; 2) Fructus Hippophae extract is prepared; 3) complex A is prepared; 4) complex B is prepared; 5) beverage is prepared.
2. beverage according to claim 1, it is characterized in that, described beverage is prepared as follows and obtains: 1) prepare Fructus Chaenomelis extract: cut seed open after being cleaned by Fructus Chaenomelis, it is cut into fragment, air-dry, it is ground into powder, is then placed in the ethanol that volume fraction is 70% and soaks 30 minutes, not being as the criterion, decompression rotary evaporation removes ethanol; Ultra-vioket radiation 15min subsequently, then add the soak with ethanol 30min that volume fraction is 85%, the then centrifugal supernatant of collecting of 1000 turns/min, the extractum that supernatant concentration becomes density be 1.2g/ml, 80 DEG C are dried, are ground into powder, to obtain final product;
2) prepare Fructus Hippophae extract: Fructus Hippophae high-speed tissue mashing machine pulled an oar, then add water boil 20min, remove slag by three layers filtered through gauze, collect filtrate and get final product; The mass ratio of described Fructus Hippophae and water is 1:3;
3) complex A is prepared: by weight ratio mix homogeneously according to 2:1 of Fructus Lycii and Radix Arctii, add the soak by water 1 hour of 5 times of weight, collect by filtration filtrate, the pH adjusting filtrate with the hydrochloric acid solution of 1mol/L is 6.5, then filtrate is condensed into the extractum that density is 1.1g/ml, 80 DEG C dried, is ground into powder and get final product;
4) prepare complex B: by Semen oryzae sativae and Semen Coicis according to the weight ratio mix homogeneously of 2:1, be then ground to Powdered, cross 200 mesh sieves and get final product;
5) beverage is prepared: put in milk by Fructus Chaenomelis extract, Fructus Hippophae extract, complex A, complex B and stabilizer, mix homogeneously, it is subsequently placed in 95 DEG C to sterilize 160 seconds, cool after 32 DEG C, add Lactobacillus bulgaricus, ferment 12 hours, terminate fermentation, finally cool to 4 DEG C, package encapsulation and get final product.
3. beverage according to claim 1 and 2, it is characterised in that the mass ratio of described Fructus Chaenomelis extract, Fructus Hippophae extract, complex A, complex B, stabilizer and milk is 3-5:5-7:1-2:3-5:1-2:100-150.
4. the beverage according to claim 1-3, it is characterised in that described stabilizer is obtained by mixing according to the mass ratio of 5:1:3 by sodium hexameta phosphate, xanthan gum and sodium alginate.
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| CN201510986191.8A CN105613736A (en) | 2015-12-25 | 2015-12-25 | Composite probiotic beverage prepared by utilizing pawpaw extract |
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| CN201510986191.8A CN105613736A (en) | 2015-12-25 | 2015-12-25 | Composite probiotic beverage prepared by utilizing pawpaw extract |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109045151A (en) * | 2018-09-29 | 2018-12-21 | 昆明医科大学第附属医院 | A kind of composition and preparation method thereof of regulation intestinal flora |
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| CN101185463A (en) * | 2006-11-16 | 2008-05-28 | 马学亮 | Fat-reducing health care breakfast milk |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109045151A (en) * | 2018-09-29 | 2018-12-21 | 昆明医科大学第附属医院 | A kind of composition and preparation method thereof of regulation intestinal flora |
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Application publication date: 20160601 |