CN105606583A - Optical fiber detection system for biological detection - Google Patents
Optical fiber detection system for biological detection Download PDFInfo
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- CN105606583A CN105606583A CN201610177761.3A CN201610177761A CN105606583A CN 105606583 A CN105606583 A CN 105606583A CN 201610177761 A CN201610177761 A CN 201610177761A CN 105606583 A CN105606583 A CN 105606583A
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- fiber
- optical fiber
- pulse signal
- fibre bundle
- computer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention belongs to the technical field of optical fiber detection, and relates to an optical fiber detection system for biological detection. The optical fiber detection system comprises a pulse signal generator, a pulse signal laser device, an optical fiber bundle assembly, a photoelectric detector, a phase lock amplifier and a computer which are connected in sequence, wherein the phase lock amplifier is further connected with the pulse signal generator; the optical fiber bundle assembly is further connected with a probe; the probe is arranged in a biological-liquid-to-be-detected detection basin; the biological-liquid-to-be-detected detection basin is provided with a liquid input end and a liquid output end; the liquid output end is connected with a peristaltic pump; and the peristaltic pump is connected with the computer. According to the optical fiber detection system, the optical fiber bundle assembly completes transmission of laser beams and collection and transmission of light signals, so that energy loss of light transmission is reduced, and the coupling efficiency of fluorescence is improved; and meanwhile, due to the compact combination and the design of the pulse signal generator, the pulse signal laser device, the optical fiber bundle assembly, the probe, the photoelectric detector, the phase lock amplifier and the computer, the signal-to-noise ratio of the equipment is reduced, the structure of the equipment is simplified, and the cost is reduced.
Description
Technical field
The invention belongs to fiber-optic probe technical field, relate to a kind of fiber-optic probe system, relate in particular to a kind of for biological detectionFiber-optic probe system.
Background technology
Fibre Optical Sensor owing to thering is the electromagnetic interference of not being subject to, volume is little, highly sensitive, speed is fast, the feature such as easy to operate developsVery fast; Fiber-optic probe device is the critical component of Fibre Optical Sensor, at aspects such as food inspection, environment measuring, medical researchesThrough being widely applied. Traditional optical fiber biosensor utilizes optical fiber to carry out optical transmission of information as probe, but receives in informationWhen collection, still utilize optical fractionation components and parts, therefore, system complex, volume is large; There is at present new Fibre Optical Sensor to replaceTraditional Fibre Optical Sensor, fiber-optic probe device in this novel optical fiber sensor adopts conical fiber and list-multimode fibre couplingClose device and combine, because this list-multi-module optical fiber coupler is to adopt a single-mode fiber and a root multimode fiber fused biconical taper systemForm, the single-mode fiber of use and multimode fibre sectional area differ hundreds of times, so the manufacture craft difficulty of coupler is very large,Yield rate is very low, and cost is very high, is unfavorable for product mass production and marketing.
In addition, the signal to noise ratio of the existing fiber-optic probe equipment for biological detection is high, detects accuracy low, and complex structure,Production cost and use cost are all higher.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of coupling efficiency that improves fluorescence is provided, has reduced signal to noise ratio,The fiber-optic probe system for biological detection that accuracy in detection is high.
The technical scheme that the present invention deals with problems is: a kind of fiber-optic probe system for biological detection, comprises that pulse signal occursDevice, pulse signal laser instrument, fibre bundle assembly, probe, photodetector, lock-in amplifier and computer, described pulse letterNumber generator, pulse signal laser instrument, fibre bundle assembly, photodetector, lock-in amplifier, computer connect successively, instituteStating lock-in amplifier is also connected with pulse signal generator; Described fibre bundle assembly comprise fibre bundle jointly end, single-mode fiber, pointProp up optical fiber, one end of described single-mode fiber, one end of branch optical fiber are connected to fibre bundle by same bundling device and jointly hold, instituteState the common end of fibre bundle and be connected with probe, the other end of described single-mode fiber is connected with pulse signal laser instrument, described branch optical fiberThe other end be connected with photodetector, described probe is arranged in biofluid detection cell to be measured, described biological fluids health check-up to be measuredSurvey pond and be provided with liquid input and liquid output, described liquid output is connected with peristaltic pump, and described peristaltic pump and computer connectConnect.
Described pulse signal generator provide frequency and pulsewidth all adjustable pulse signal to pulse signal laser instrument; Described pulse letterNumber laser instrument is transferred to fibre bundle assembly by optical signal by single-mode fiber; Optical signal is outputed to probe by described fibre bundle assembly,And the fluorescence of probe feedback is outputed to photodetector; Optical signal is converted to the signal of telecommunication by described photodetector, and pass toLock-in amplifier; The frequency values of the pulse signal of described lock-in amplifier recording pulse signal generator, and detect with pulse and believeThe output signal of number generator same frequency value; Described computer control lock-in amplifier, records its output signal.
Described peristaltic pump, for regulating the flow of liquid, makes liquid flow steady.
Further, described probe is fluorescent probe.
Further, described peristaltic pump is connected with computer by holding wire.
Further, described single-mode fiber and branch optical fiber are glass optical fiber.
Further, the scope of the diameter of described single-mode fiber and branch optical fiber is 100mm～600mm.
Preferably, the scope of the diameter of described single-mode fiber and branch optical fiber is 200mm～500mm.
Further, described pulse signal generator, pulse signal laser instrument, fibre bundle assembly, photodetector, phase-locked puttingLarge device and computer are integrated in same suitcase, and described suitcase is provided with slot, and described probe energy detachable is arranged on slot;Portable structural design can be convenient for carrying the fiber-optic probe system for biological detection of the present invention, and described probeCan conveniently pick and place in use.
Further, the volume of described suitcase is 0.02m3～0.08m3。
Beneficial effect of the present invention is: the present invention completes the transmission of laser beam and the collection of optical signal, transmission by fibre bundle assembly,Reduce the energy loss of light transmission, improved the coupling efficiency of fluorescence, simultaneously by pulse signal generator, pulse signal laserCompactness combination and the design of device, fibre bundle assembly, probe, photodetector, lock-in amplifier and computer, reduced equipmentSignal to noise ratio, simplified the structure of equipment, reduced cost; Improve the accuracy detecting; And compact conformation of the present invention,Portable flexibly, easy to use.
Brief description of the drawings
Fig. 1 is the structural representation of the fiber-optic probe system for biological detection of the present invention;
Fig. 2 is the structural representation of the fibre bundle assembly shown in Fig. 1.
In figure: 1-pulse signal generator; 2-pulse signal laser instrument; 3-fibre bundle assembly, 31-fibre bundle is held jointly,32-single-mode fiber, 33-branch optical fiber, 34-bundling device; 4-probe; 5-photodetector; 6-lock-in amplifier; 7-meterCalculation machine; 8-peristaltic pump; 9-biofluid detection cell to be measured, 91-liquid input, 92-liquid output.
Arrow in Fig. 1 represents the flow direction of biofluid.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the present invention is further illustrated.
Embodiment mono-
As depicted in figs. 1 and 2, a kind of fiber-optic probe system for biological detection, comprises pulse signal generator 1, pulseSignal laser 2, fibre bundle assembly 3, probe 4, photodetector 5, lock-in amplifier 6 and computer 7, described pulseSignal generator 1, pulse signal laser instrument 2, fibre bundle assembly 3, photodetector 5, lock-in amplifier 6, computer 7Connect successively, described lock-in amplifier 6 is also connected with pulse signal generator 1; Described fibre bundle assembly 3 comprises that fibre bundle altogetherWith end 31, single-mode fiber 32, branch optical fiber 33, one end of described single-mode fiber 32, one end of branch optical fiber 33 are by sameA bundling device 34 is connected to the common end 31 of fibre bundle, and the common end 31 of described fibre bundle is connected with probe 4, described single-mode fiber32 the other end is connected with pulse signal laser instrument 2, and the other end of described branch optical fiber 33 is connected with photodetector 5, instituteState probe 4 and be arranged in biofluid detection cell 9 to be measured, described biofluid detection cell 9 to be measured is provided with liquid input 91 HesLiquid output 92, described liquid output 92 is connected with peristaltic pump 8, and described peristaltic pump 8 is connected with computer 7.
Described pulse signal generator 1 provide frequency and pulsewidth all adjustable pulse signal to pulse signal laser instrument 2; Described arteries and veinsRush signal laser 2 optical signal is transferred to fibre bundle assembly 3 by single-mode fiber 32; Described fibre bundle assembly 3 is by optical signalOutput to probe 4, and the fluorescence of probe 4 feedbacks is outputed to photodetector 5; Described photodetector 5 is changed optical signalFor the signal of telecommunication, and pass to lock-in amplifier 6; The pulse signal of described lock-in amplifier 6 recording pulse signal generators 1Frequency values, and detect and the output signal of pulse signal generator 1 same frequency value; Described computer 7 is controlled lock-in amplifier6, record its output signal.
Described peristaltic pump 8, for regulating fluid flow, makes liquid flow steady.
Described probe 4 is fluorescent probe.
Described peristaltic pump 8 is connected with computer 7 by holding wire.
Described single-mode fiber 32 and branch optical fiber 33 are glass optical fiber.
The scope of the diameter of described single-mode fiber 32 and branch optical fiber 33 is 100mm～600mm.
Described pulse signal generator 1, pulse signal laser instrument 2, fibre bundle assembly 3, photodetector 5, lock-in amplifier6 and computer 7 be integrated in same suitcase (not shown), described suitcase is provided with slot, described probe 4 can be torn openThe formula of unloading is arranged on slot.
The volume of described suitcase is 0.02m3～0.08m3。
Embodiment bis-
In the present embodiment, the scope of the diameter of described single-mode fiber 32 and branch optical fiber 33 is 200mm～500mm, removesOutside this, other structure is identical with embodiment mono-.
The present invention is not limited to above-mentioned embodiment, and in the situation that not deviating from flesh and blood of the present invention, those skilled in the art canAll fall into protection scope of the present invention with any distortion, improvement, the replacement expected.
Claims (8)
1. for a fiber-optic probe system for biological detection, it is characterized in that, comprise that pulse signal generator, pulse signal swashLight device, fibre bundle assembly, probe, photodetector, lock-in amplifier and computer, described pulse signal generator, pulseSignal laser, fibre bundle assembly, photodetector, lock-in amplifier, computer connect successively, and described lock-in amplifier alsoBe connected with pulse signal generator; Described fibre bundle assembly comprises fibre bundle end, single-mode fiber, branch optical fiber jointly, described listOne end of mode fiber, one end of branch optical fiber are connected to fibre bundle by same bundling device and jointly hold, and described fibre bundle is held jointlyBe connected with probe, the other end of described single-mode fiber is connected with pulse signal laser instrument, the other end of described branch optical fiber and photoelectricityDetector is connected, and described probe is arranged in biofluid detection cell to be measured, and it is defeated that described biofluid detection cell to be measured is provided with liquidEnter end and liquid output, described liquid output is connected with peristaltic pump, and described peristaltic pump is connected with computer.
2. the fiber-optic probe system for biological detection according to claim 1, is characterized in that, described probe is fluorescenceProbe.
3. the fiber-optic probe system for biological detection according to claim 1, is characterized in that, described peristaltic pump passes throughHolding wire is connected with computer.
4. the fiber-optic probe system for biological detection according to claim 1, is characterized in that, described single-mode fiber andBranch optical fiber is glass optical fiber.
5. the fiber-optic probe system for biological detection according to claim 4, is characterized in that, described single-mode fiber andThe scope of the diameter of branch optical fiber is 100mm～600mm.
6. the fiber-optic probe system for biological detection according to claim 4, is characterized in that, described single-mode fiber andThe scope of the diameter of branch optical fiber is 200mm～500mm.
7. the fiber-optic probe system for biological detection according to claim 1, is characterized in that, described pulse signal is sent outRaw device, pulse signal laser instrument, fibre bundle assembly, photodetector, lock-in amplifier and computer are integrated in same portableIn case, described suitcase is provided with slot, and described probe energy detachable is arranged on slot.
8. the fiber-optic probe system for biological detection according to claim 7, is characterized in that the body of described suitcaseAmass as 0.02m3～0.08m3。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610177761.3A CN105606583A (en) | 2016-03-25 | 2016-03-25 | Optical fiber detection system for biological detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610177761.3A CN105606583A (en) | 2016-03-25 | 2016-03-25 | Optical fiber detection system for biological detection |
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| CN105606583A true CN105606583A (en) | 2016-05-25 |
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| CN201610177761.3A Pending CN105606583A (en) | 2016-03-25 | 2016-03-25 | Optical fiber detection system for biological detection |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105784605A (en) * | 2016-04-05 | 2016-07-20 | 中国科学院南海海洋研究所 | Shore-based hermatypic coral temporary culture and spectral measurement device |
| CN106841141A (en) * | 2017-01-26 | 2017-06-13 | 浙江大学 | A kind of fiber optic loop battle array resonance type piezoelectric scanning method and device based on photon restructuring |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1873450A (en) * | 2006-06-30 | 2006-12-06 | 清华大学 | Biosensor of full fiber optic evanescent wave |
| CN101666747A (en) * | 2008-09-04 | 2010-03-10 | 北京金达清创环境科技有限公司 | Array fibre evanescent wave biosensor system |
| CN103245641A (en) * | 2012-02-10 | 2013-08-14 | 清华大学 | Multi-channel planar waveguide evanescent wave biosensor |
| CN105115955A (en) * | 2015-09-16 | 2015-12-02 | 北京首量科技有限公司 | Optical fiber detection device for biological detection |
| CN105424663A (en) * | 2015-11-24 | 2016-03-23 | 西南大学 | Method for detecting phthalic acid ester compound concentration based on optical fiber immunosense |
| CN205426791U (en) * | 2016-03-25 | 2016-08-03 | 北京首量科技有限公司 | A optic fibre detection system for biological detection |
-
2016
- 2016-03-25 CN CN201610177761.3A patent/CN105606583A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1873450A (en) * | 2006-06-30 | 2006-12-06 | 清华大学 | Biosensor of full fiber optic evanescent wave |
| CN101666747A (en) * | 2008-09-04 | 2010-03-10 | 北京金达清创环境科技有限公司 | Array fibre evanescent wave biosensor system |
| CN103245641A (en) * | 2012-02-10 | 2013-08-14 | 清华大学 | Multi-channel planar waveguide evanescent wave biosensor |
| CN105115955A (en) * | 2015-09-16 | 2015-12-02 | 北京首量科技有限公司 | Optical fiber detection device for biological detection |
| CN105424663A (en) * | 2015-11-24 | 2016-03-23 | 西南大学 | Method for detecting phthalic acid ester compound concentration based on optical fiber immunosense |
| CN205426791U (en) * | 2016-03-25 | 2016-08-03 | 北京首量科技有限公司 | A optic fibre detection system for biological detection |
Non-Patent Citations (2)
| Title |
|---|
| 刘利秋主编: "《传感器原理与应用》", 31 August 2015 * |
| 龙峰等: "倏逝波全光纤免疫传感器的开发及性能研究", 《倏逝波全光纤免疫传感器的开发及性能研究》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105784605A (en) * | 2016-04-05 | 2016-07-20 | 中国科学院南海海洋研究所 | Shore-based hermatypic coral temporary culture and spectral measurement device |
| CN106841141A (en) * | 2017-01-26 | 2017-06-13 | 浙江大学 | A kind of fiber optic loop battle array resonance type piezoelectric scanning method and device based on photon restructuring |
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Address after: 101111, Beijing, Tongzhou District city channel light mechanical and electrical integration industrial base, four light street, No. 2, building 304, room 5 Applicant after: Beijing Polytron Technologies Inc Address before: 101111 Tongzhou District City, Zhongguancun science and Technology Park, Tongzhou Industrial Park, the integration of optical and electrical integration of industrial base, Xing Guang street, No. four, No. 5 Applicant before: Beijing Scitlion Technology Co., Ltd. |
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Application publication date: 20160525 |