CN105606551B - A kind of absorbance mass measuring method of nephelometry titer of antibodies analyzer - Google Patents
A kind of absorbance mass measuring method of nephelometry titer of antibodies analyzer Download PDFInfo
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- CN105606551B CN105606551B CN201610066314.0A CN201610066314A CN105606551B CN 105606551 B CN105606551 B CN 105606551B CN 201610066314 A CN201610066314 A CN 201610066314A CN 105606551 B CN105606551 B CN 105606551B
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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Abstract
The present invention relates to the absorbance mass measuring method of nephelometry titer of antibodies analyzer, can effectively solve the variability issues of absorbance between the error of indication of nephelometry titer of antibodies analyzer absorbance measuring, each passage, method is, neutral gray glass is selected as optical filter, optical filter is attached separately in filter supporter, is assembled into 4 kinds of spectral neutrality optical filters；Measure spectrum neutral colour filter absorbance standard value, assemble spectral neutrality optical filter, measure the titer of antibodies analyzer absorbance error of indication, measure titer of antibodies analyzer interchannel absorbance difference, the inventive method is reliable and stable, measurement accuracy is high, efficiently solve the error of indication measurement of nephelometry titer of antibodies analyzer absorbance measuring result and the otherness measurement problem of each interchannel measurement result, it is not only suitable for the exfactory inspection of nephelometry titer of antibodies analyzer, it is also applied for quality-monitoring of the measurement technology mechanism to nephelometry titer of antibodies analyzer.
Description
Technical field
The present invention relates to mass measurement, particularly a kind of absorbance mass measurement side of nephelometry titer of antibodies analyzer
Method.
Background technology
《Pharmacopoeia of People's Republic of China (2015 editions)》Regulation, nephelometry are a kind of measurement sides of titer of antibodies measure
Method.According to pharmacopeia, relevant enterprise have developed nephelometry titer of antibodies analyzer, and the instrument is that liquid is trained using antibiotic
The inhibitory action of bacteria growing is tested in nutrient solution, by measuring the fluid nutrient medium for adding standard sample and adding sample respectively
Absorbance size, to determine the instrument of sample titer of antibodies.
Nephelometry titer of antibodies analyzer measurement wavelength is Single wavelength, according to user's needs, is equipped with 530nm or 580nm
Monochromatic light source.《Spectrophotometer etalon national metrological verification regulations》It is to the requirement of first-order spectrum neutral colour filter：
Transmittance positive and negative difference≤0.001, transmittance uniformity≤0.001.《Titer of antibodies analyzer national measurement control gauge
Model》It is to the requirement of the absorbance error of indication of nephelometry titer of antibodies analyzer：Absorbance≤0.03, interchannel absorbance are poor
Different requirement is：Absorbance≤0.03.
The solwution method of absorbance mass measuring method is just gradually being replaced by spectral neutrality optical filter at present.Such as it is divided light
It is respectively 0.5,0.7,1.0 to spend the absorbance mass measurement counted using the absorbance trade mark, and the spectral neutrality that light path is 10mm filters
Piece, the absorbance mass measurement of enzyme micro-plate reader are respectively 0.2,0.5,1.0,1.5 using the absorbance trade mark, and light path is 10mm's
Spectral neutrality optical filter.But the light path of nephelometry titer of antibodies analyzer is 20mm, above spectral neutrality optical filter does not apply to
In the absorbance mass measurement of nephelometry titer of antibodies analyzer.
The absorbance mass measuring method of existing nephelometry titer of antibodies analyzer uses solwution method more, i.e., will be same molten
Liquid (such as cobalt chloride solution) is added in the cuvette of nephelometry titer of antibodies analyzer, respectively in nephelometry titer of antibodies
Absorbance measurement is carried out on the different passages of analyzer, to control the otherness of different interchannels.Solwution method is uncontrollable every
The absorbance error of indication of individual passage, the inhomogeneities of solution and the unholiness property of cuvette also influence whether absorbance matter in addition
Measurement.Therefore, to ensure the quality of nephelometry titer of antibodies analyzer absorbance measuring result, it is necessary to develop new turbidity
The absorbance mass measuring method of method titer of antibodies analyzer.
The content of the invention
For the above situation, to overcome the defect of prior art, the purpose of the present invention is just to provide a kind of nephelometry antibiosis
The absorbance mass measuring method of plain titration instrument, can effectively solve nephelometry titer of antibodies analyzer absorbance measuring
The variability issues of absorbance between the error of indication, each passage.
The technical scheme that the present invention solves is realized by following steps：
(1) optical filter, is selected：
Select 4 kinds of neutral gray glass to be made optical filter first, the glass trade mark be respectively ZAB50, ZAB30, ZAB20,
ZAB10, the corresponding absorbance trade mark are respectively 0.3,0.5,0.7,1.0, and every kind of optical filtering length of a film is 56-60mm, wide 16-
20mm, thick 1.8-2.2mm, transmittance positive and negative difference≤0.001, transmittance uniformity≤0.001；
(2) spectral neutrality optical filter, is assembled：
Optical filter made of 4 kinds of neutral gray glass is attached separately in filter supporter, filter supporter is aluminum, table
Face blacks, and stent size size is identical with the cuvette of nephelometry titer of antibodies analyzer, has perforate on support, it is ensured that turbidity
The light that method titer of antibodies analyzer is sent lies against opening for 4 supports respectively completely by support, by 4 kinds of different optical filters
At hole, optical filter is fixed with the spring leaf not being in the light, is assembled into 4 kinds of spectral neutrality optical filters；
Described filter supporter is made up of the support body 6 of aluminum, and the top both sides of support body 6 have for filter supporter to be hanged
The plush copper 1 on the culturing rack of nephelometry titer of antibodies analyzer is hung over, there are the elongate recesses for keeping flat optical filter in support body
2, groove centre is provided with the perforate 3 of square, has spring leaf fixing hole 4 on the support body on the top of groove 2, there is opening spring leaf bottom
5, the fixing hole 9 corresponding with spring leaf fixing hole 4 is arranged at the top of spring leaf 7, and bottom is provided with corresponding slotted eye consistent with perforate 3
8, optical filter is lain against in groove 2, and spring leaf 7 is pressed in above optical filter, and spring leaf is through fixing hole 9, spring leaf fixing hole 4 by spiral shell
Spring leaf is fixed on groove 2 by nail, filter set is formed a piece of spectral neutrality filter sheet structure on support；
(3), measure spectrum neutral colour filter absorbance standard value：
Absorbance measurement is carried out in 530nm and 580nm respectively to 4 kinds of spectral neutrality optical filters with spectrophotometer,
530nm measured value is the spectral neutrality optical filter 530nm absorbance standard values, is the spectral neutrality in 580nm measured value
Optical filter 580nm absorbance standard values；
(4) spectral neutrality optical filter, is assembled：
Each spectral neutrality filter set includes 8 spectral neutrality optical filters：The ZAB50 that the absorbance trade mark is 0.3 is neutral
ZAB20 neutral gray glass optical filter that smoked glass optical filter, the absorbance trade mark are 0.7, the absorbance trade mark are 1.0
Each 1 of ZAB100 neutral gray glass optical filters, the absorbance trade mark are 0.5 ZAB30 neutral gray glass optical filter 5, are inhaled
The ZAB30 neutral gray glass that ZAB50 neutral gray glass optical filter that the luminosity trade mark is 0.3, the absorbance trade mark are 0.5 filters
The ZAB10 neutral gray glass that ZAB20 neutral gray glass optical filter that piece, the absorbance trade mark are 0.7, the absorbance trade mark are 1.0
Each 1 of glass optical filter, the measurement for the nephelometry titer of antibodies analyzer absorbance error of indication；The absorbance trade mark is 0.5
ZAB30 neutral gray glass optical filter 5, the survey for nephelometry titer of antibodies analyzer interchannel absorbance difference
Amount；
(5), the titer of antibodies analyzer absorbance error of indication measures：
The ZAB30 for being 0.5 by ZAB50 neutral gray glass optical filter that the absorbance trade mark is 0.3, the absorbance trade mark is neutral
ZAB20 neutral gray glass optical filter that smoked glass optical filter, the absorbance trade mark are 0.7, the absorbance trade mark are 1.0
4 spectral neutrality optical filters of ZAB100 neutral gray glass optical filters, which are respectively perpendicular, is placed in nephelometry titer of antibodies analyzer
Measured in sample cell, the maximum of the difference of 4 spectral neutrality optical filter absorbance measurements and standard value resists for nephelometry
The raw plain titration instrument absorbance error of indication, the absorbance error of indication≤0.03；
(6), titer of antibodies analyzer interchannel absorbance difference measures：
The ZAB30 neutral gray glass optical filters that 5 absorbance trades mark are 0.5 are respectively perpendicular and are placed in nephelometry antibiotic
Measured in each passage sample cell of titration instrument, maximum of 5 spectral neutrality optical filters in each passage absorbance measurement
The difference of value and minimum value is interchannel absorbance difference, interchannel absorbance difference≤0.03.
The inventive method is reliable and stable, and measurement accuracy is high, efficiently solves nephelometry titer of antibodies analyzer absorbance
The error of indication measurement of measurement result and the otherness measurement problem of each interchannel measurement result, are not only suitable for nephelometry antibiosis
The exfactory inspection of plain titration instrument, it is also applied for measurement technology mechanism and the quality of nephelometry titer of antibodies analyzer is supervised
Survey, there is an actual application value, economic and social profit is notable.
Brief description of the drawings
Fig. 1 is the filter supporter structural front view of the present invention.
Fig. 2 is the filter supporter profile of the present invention.
Fig. 3 is the filter supporter structure upward view of the present invention.
Fig. 4 is the filter supporter chip architecture of the spring front view of the present invention.
Embodiment
Elaborated below in conjunction with the embodiment of the concrete condition present invention.
The present invention can be realized in specific implementation by following steps：
(1) optical filter selects：
Optical filter is 4 kinds of neutral gray glass, and the glass trade mark is respectively ZAB50, ZAB30, ZAB20, ZAB10, corresponding
The absorbance trade mark is respectively 0.3,0.5,0.7,1.0, and every kind of optical filter is thick 2mm, long 58mm, wide 18mm；
With meeting《Ultraviolet, visible, near infrared spectrometer national metrological verification regulations》The spectrophotometer of I levels requirement
Every tablet filter is measured in 530nm and 580nm transmittance positive and negative difference, transmittance uniformity respectively；
Spectrophotometer measurement wavelength is first set to 530nm, spectral bandwidth 2nm, reference is done with air, by spectrophotometer
School zero, it is 1.000 to make its transmittance, and 1 tablet filter is positioned in light path, vertical with light path, and light path is by optical filter front
Center position, transmittance values being read, continuous measure 3 times is averaged, and is optical filter 530nm front transmittance values,
Light path is passed through into optical filter reverse side center position again, reads transmittance values, continuous measure 3 times is averaged, and is the optical filtering
The absolute value of piece 530nm reverse side transmittance values, optical filter 530nm front transmittance values and the difference of reverse side transmittance values is
Spectrophotometer measurement wavelength is set to 580nm and measured in 530nm transmittance positive and negative difference by the optical filter, is calculated
The optical filter is in 580nm transmittance positive and negative difference, transmittance positive and negative difference of every tablet filter in 530nm and 580nm
≤ 0.001；
According to above step, carried out respectively at upper and lower each 20mm of every tablet filter center position 530nm and
580nm positive and negative transmittance values measurement；During wavelength 530nm, difference of the optical filter in the position transmittance measured value of upper, middle and lower 3
Absolute value maximum for the optical filter in 530nm transmittance uniformities；During wavelength 580nm, optical filter is upper, middle and lower 3
The maximum for putting the absolute value of the difference of transmittance measured value exists for the optical filter in 580nm transmittance uniformities, every tablet filter
530nm and 580nm transmittances uniformity is ≤0.001；
(2) spectral neutrality optical filter assembles：
Filter supporter is made, for the aluminium brackets (see Fig. 1) of surface blackening processing, support body long 22mm, wide 22mm are high
96mm, away from the plush copper 1 for having wide 3mm at 12mm below pedestal upper end, for filter supporter to be suspended on into nephelometry antibiotic effect
There is long 58mm on the culturing rack of valency analyzer, in support, wide 18mm, deep 11mm elongate recesses 2 are recessed for keeping flat optical filter
There are long 52mm, wide 14mm on groove, both ends are semicircle, and centre is square perforate 3；
The apparent size of spring leaf (such as Fig. 4) is long 54.5mm, wide 17mm, thick 0.5mm, and slotted eye is long 34mm, wide 14mm,
Top is semicircle, and there is the opening 5 of square bottom；
1 tablet filter is lain against in the groove 2 in 1 support, spring leaf is lain on optical filter, in the spring of support
Spring leaf is fastened with screw at piece fixing hole 4, optical filter is fixed on support, is assembled into 1 spectral neutrality optical filter (4
The assemble method of kind optical filter is identical, does not describe one by one)；
(3), spectral neutrality optical filter absorbance standard value measures：
With meeting《Ultraviolet, visible, near infrared spectrometer national metrological verification regulations》The spectrophotometer of I levels requirement
Every spectral neutrality optical filter is measured in 530nm and 580nm absorbance standard value respectively；
It is 530nm to adjust spectrophotometer measurement wavelength, spectral bandwidth 2nm, reference is done with air, by spectrophotometer school
Zero, it is 0.000 to make its absorbance, and 1 spectral neutrality optical filter is positioned in the light path of spectrophotometer, vertical with light path,
Light path passes through spectral neutrality optical filter front center point position, reads absorbance, and continuous measure 3 times is averaged, as should
Spectral neutrality optical filter 530nm front absorbance；Light path is passed through into spectral neutrality optical filter reverse side center position again, read
Go out absorbance, continuous measure 3 times is averaged, and is spectral neutrality optical filter 530nm reverse side absorbance；The spectrum
The 530nm of neutral colour filter front absorbance is that the spectral neutrality optical filter exists with the average value of reverse side absorbance
530nm absorbance standard value, spectrophotometer measurement wavelength is set to 580nm and carries out above measurement, calculates the spectral neutrality
Absorbance standard value of the optical filter in 580nm；
(4), spectral neutrality filter set is matched somebody with somebody：
Every group of spectral neutrality optical filter includes 8 spectral neutrality optical filters, and the wherein absorbance trade mark is 0.3,0.7,1.0
Each 1 of optical filter, the absorbance trade mark are 0.5 optical filter 5, and the absorbance trade mark is 0.3,0.5,0.7,1.0 each 1 spectrum
The error of indication that neutral colour filter is used for nephelometry titer of antibodies analyzer absorbance measures, and the absorbance trade mark is 5 of 0.5
Spectral neutrality optical filter 530nm absorbance standard values difference should≤0.003,580nm absorbance standard values difference≤
0.003, the measurement for nephelometry titer of antibodies analyzer interchannel absorbance difference；
(5), the instrument absorbance error of indication measures：
By nephelometry titer of antibodies analyzer under the conditions of unobstructed light path, using air as reference, by the absorbance trade mark
It is respectively perpendicular for 0.3,0.5,0.7,1.0 4 spectral neutrality optical filters and is placed in nephelometry titer of antibodies analyzer sample cell
In, absorbance is continuously measured per tablet filter average for 3 times and filtered for the optical filter absorbance measurement, 4 spectral neutralities
The maximum of the difference of piece absorbance measurement and standard value is the absorbance error of indication of the nephelometry titer of antibodies analyzer,
The absorbance error of indication≤0.03；
(6), absorbance difference measures between instrument channel：
By nephelometry titer of antibodies analyzer under the conditions of unobstructed light path, using air as reference, by the absorbance trade mark
It is respectively perpendicular and is placed in each passage sample cell of nephelometry titer of antibodies analyzer for 0.5 5 spectral neutrality optical filters, each
Passage continuously measures absorbance and averaged as the passage absorbance measurement, the maximum of each passage absorbance measurement for 3 times
Interchannel absorbance difference of the value with the difference of minimum value for the nephelometry titer of antibodies analyzer, interchannel absorbance difference≤
0.03。
The present invention through on the spot apply with calculating, achieve very satisfied advantageous effects, relevant information is as follows：
The present invention through applying and test, described optical filter on the spot：
The neutral gray glass of selection is for the glass trade mark that Beijing Yongxing perception information Technology Co., Ltd. produces
ZAB50, ZAB30, ZAB20, ZAB10 4 kinds of neutral gray glass, thick 2mm, the corresponding absorbance trade mark is respectively 0.3,0.5,
0.7th, 1.0, absorption spectrum of every kind of neutral gray glass in the range of wavelength 500nm~600nm is smoothed curve.Will be every kind of
Neutral gray glass cut growth 58mm, wide 18mm optical filter.With I levels 5000 types of Cary are ultraviolet, visible, near-infrared is divided
Spectrophotometer measures to the transmittance values of each optical filter, and the maximum of transmittance positive and negative difference is 0.0007, transmission
Maximum than uniformity difference is 0.0008, meets the requirement of first-order spectrum neutral colour filter；
Described spectral neutrality optical filter assembling：Filter supporter is made, support is aluminum, surface blackening, stent size
Size is identical with nephelometry titer of antibodies analyzer cuvette, there is perforate on support, it is ensured that nephelometry titer of antibodies determines
The light that instrument light source is sent passes through support completely；Spring leaf is made, for optical filter to be fixed on into filter supporter；By 4 kinds not
Same optical filter is lain against on the trough of 4 supports respectively, is fixed with spring leaf, spring leaf is not in the light, and is assembled into 4 kinds of spectrum
Property optical filter.
Described spectral neutrality optical filter absorbance standard value measurement：
With I levels 5000 types of Cary are ultraviolet, visible, near-infrared is divided spectrophotometer respectively in 530nm and 580nm to each
Spectral neutrality optical filter absorbance standard value measures, and measurement result see the table below；
Described spectral neutrality filter set is matched somebody with somebody：Each spectral neutrality filter set includes 8 spectral neutrality optical filters, its
The middle absorbance trade mark is 0.3,0.7,1.0 each 1 of spectral neutrality optical filter, and the spectral neutrality that the absorbance trade mark is 0.5 filters
Piece 5,5 spectral neutrality optical filters that the absorbance trade mark is 0.5 in the group are in the difference of 530nm absorbance standard values
0.001, it is 0.002 (seeing the above table) in the difference of 580nm absorbance standard values, respectively less than 0.003, meet the requirements.
Described nephelometry titer of antibodies analyzer absorbance error of indication measurement：
By certain nephelometry titer of antibodies analyzer under the conditions of unobstructed light path, using air as reference, by absorbance
4 spectral neutrality optical filters that the trade mark is 0.3,0.5,0.7,1.0, which are respectively perpendicular, is placed in this nephelometry titer of antibodies measure
In instrument sample cell, continuously measure 3 times and averaged as the optical filter absorbance measurement, this nephelometry antibiosis per tablet filter
Plain titration instrument see the table below to 4 spectral neutrality optical filter absorbance measurements.Nephelometry titer of antibodies analyzer is to 4
The maximum of the difference of spectral neutrality optical filter absorbance measurement and spectral neutrality optical filter absorbance standard value is this turbidity
The method titer of antibodies analyzer absorbance error of indication, the nephelometry titer of antibodies analyzer absorbance error of indication for-
0.018, less than 0.03, show that absorbance Measurement Result meets the requirements；
Absorbance difference measures between described instrument channel：
By certain nephelometry titer of antibodies analyzer under the conditions of unobstructed light path, using air as reference, by absorbance
5 spectral neutrality optical filters that the trade mark is 0.5, which are respectively perpendicular, is placed in each passage sample of this nephelometry titer of antibodies analyzer
In groove, each passage is continuously measured 3 times and averaged as the instrument passage absorbance measurement, each passage absorbance measurement
Maxima and minima difference between instrument channel absorbance difference, each passage of this nephelometry titer of antibodies analyzer
Absorbance measurement see the table below, and interchannel absorbance difference is 0.008, and less than 0.03, absorbance is poor between showing the instrument channel
Different measurement result meets the requirements.
From the above situation, the present invention is selected by optical filter, spectral neutrality optical filter assembles, spectral neutrality optical filter
The measurement of absorbance standard value, spectral neutrality filter set are matched somebody with somebody, the instrument absorbance error of indication measures, absorbance is poor between instrument channel
Different measurement, it is respectively ZAB50, ZAB30, ZAB20, ZAB10 4 kinds of neutral gray glass using the glass trade mark, is fabricated to spectrum
Neutral colour filter group, prove that the invention effectively solves nephelometry titer of antibodies analyzer absorbance indicating value and missed by example
Problem, the inventions such as the measurement of each interchannel absorbance difference of measurement and nephelometry titer of antibodies analyzer of difference are not only fitted
For the exfactory inspection of nephelometry titer of antibodies analyzer, it is also applied for measurement technology mechanism and nephelometry titer of antibodies is surveyed
Determine the quality-monitoring of instrument, there is an actual application value, economic and social profit is notable.
Claims (3)
1. a kind of absorbance mass measuring method of nephelometry titer of antibodies analyzer, it is characterised in that real by following steps
It is existing：
（1）, selection optical filter：
4 kinds of neutral gray glass are selected to be made optical filter first, the glass trade mark is respectively ZAB50, ZAB30, ZAB20, ZAB10,
The corresponding absorbance trade mark is respectively 0.3,0.5,0.7,1.0, and every kind of optical filtering length of a film is that 56-60 mm, width are 16-20
Mm, thickness are 1.8-2.2 mm, transmittance positive and negative difference≤0.001, transmittance uniformity≤0.001；
（2）, assembling spectral neutrality optical filter：
Optical filter made of 4 kinds of neutral gray glass is attached separately in filter supporter, filter supporter is aluminum, surface hair
Black, stent size size is identical with the cuvette of nephelometry titer of antibodies analyzer, has perforate on support, it is ensured that nephelometry resists
The light that raw plain titration instrument is sent lies against 4 kinds of different optical filters the perforate of 4 supports respectively completely by support
Place, fixes optical filter with the spring leaf not being in the light, is assembled into 4 kinds of spectral neutrality optical filters；
（3）, measure spectrum neutral colour filter absorbance standard value：
Absorbance measurement is carried out in 530 nm and 580 nm respectively to 4 kinds of spectral neutrality optical filters with spectrophotometer, 530
Nm measured value is the nm absorbance standard values of spectral neutrality optical filter 530, is filtered in 580 nm measured value for the spectral neutrality
The nm absorbance standard values of mating plate 580；
（4）, assemble spectral neutrality optical filter：
Each spectral neutrality filter set includes 8 spectral neutrality optical filters：The absorbance trade mark is 0.3 ZAB50 neutral grays
In the ZAB10 that ZAB20 neutral gray glass optical filter that glass filter, the absorbance trade mark are 0.7, the absorbance trade mark are 1.0
Property each 1 of smoked glass optical filter, the absorbance trade mark be 0.5 ZAB30 neutral gray glass optical filter 5, the absorbance trade mark
The ZAB30 neutral gray glass optical filter for being 0.5 for 0.3 ZAB50 neutral gray glass optical filter, the absorbance trade mark, extinction
Spend the trade mark be 0.7 ZAB20 neutral gray glass optical filter, the absorbance trade mark be 1.0 ZAB10 neutral gray glass optical filters
Each 1, the measurement for the nephelometry titer of antibodies analyzer absorbance error of indication；The absorbance trade mark is in 0.5 ZAB30
Property smoked glass optical filter 5, the measurement for nephelometry titer of antibodies analyzer interchannel absorbance difference；
（5）, the titer of antibodies analyzer absorbance error of indication measurement：
The ZAB30 neutral grays for being 0.5 by ZAB50 neutral gray glass optical filter that the absorbance trade mark is 0.3, the absorbance trade mark
In the ZAB10 that ZAB20 neutral gray glass optical filter that glass filter, the absorbance trade mark are 0.7, the absorbance trade mark are 1.0
4 spectral neutrality optical filters of property smoked glass optical filter, which are respectively perpendicular, to be placed in nephelometry titer of antibodies analyzer sample cell
Measure, the maximum of the difference of 4 spectral neutrality optical filter absorbance measurements and standard value is nephelometry titer of antibodies
The analyzer absorbance error of indication, the absorbance error of indication≤0.03；
（6）, titer of antibodies analyzer interchannel absorbance difference measurement：
The ZAB30 neutral gray glass optical filters that 5 absorbance trades mark are 0.5 are respectively perpendicular and are placed in nephelometry titer of antibodies
Measured in each passage sample cell of analyzer, 5 spectral neutrality optical filters each passage absorbance measurement maximum with
The difference of minimum value is interchannel absorbance difference, interchannel absorbance difference≤0.03.
2. the absorbance mass measuring method of nephelometry titer of antibodies analyzer according to claim 1, its feature exist
In described filter supporter is the support body by aluminum（6）Form, support body（6）Top both sides have for filter supporter to be hanged
Hang over the plush copper on the culturing rack of nephelometry titer of antibodies analyzer（1）, have for keeping flat the elongated recessed of optical filter in support body
Groove（2）, groove centre is provided with the perforate of square（3）, groove（2）There is spring leaf fixing hole on the support body on top（4）, spring leaf
There is opening bottom（5）, spring leaf（7）Top has and spring leaf fixing hole（4）Corresponding fixing hole（9）, bottom is provided with and opened
Hole（3）Slotted eye corresponding to consistent（8）, optical filter lies against groove（2）In, spring leaf（7）It is pressed in above optical filter, spring leaf
Through fixing hole（9）, spring leaf fixing hole（4）Spring leaf is fixed on groove by screw（2）On, filter set is mounted in support
On, form a piece of spectral neutrality filter sheet structure.
3. the absorbance mass measuring method of nephelometry titer of antibodies analyzer according to claim 1, its feature exist
In being realized by following steps：
（1）Optical filter selects：
Optical filter is 4 kinds of neutral gray glass, and the glass trade mark is respectively ZAB50, ZAB30, ZAB20, ZAB10, corresponding extinction
It is respectively 0.3,0.5,0.7,1.0 to spend the trade mark, and every kind of optical filter is 2 mm of thickness, long 58 mm, wide 18 mm；
With meeting《Ultraviolet, visible, near infrared spectrometer national metrological verification regulations》The spectrophotometer of I levels requirement is to every
Tablet filter measures in 530 nm and 580 nm transmittance positive and negative difference, transmittance uniformity respectively；
Spectrophotometer measurement wavelength is first set to 530 nm, the nm of spectral bandwidth 2, reference is done with air, by spectrophotometer school
Zero, it is 1.000 to make its transmittance, and 1 tablet filter is positioned in light path, vertical with light path, and light path is passed through in optical filter front
Heart point position, transmittance values being read, continuous measure 3 times is averaged, and is the optical filter 530 nm front transmittance values, then
Light path is passed through into optical filter reverse side center position, reads transmittance values, continuous measure 3 times is averaged, and is the optical filter
The absolute value of 530 nm reverse side transmittance values, the optical filter 530 nm front transmittance values and the difference of reverse side transmittance values is
Spectrophotometer measurement wavelength is set to 580 nm and measured in 530 nm transmittance positive and negative difference by the optical filter, is counted
Transmittance positive and negative difference of the optical filter in 580 nm is calculated, per tablet filter in 530 nm and 580 nm transmittance positive and negative
Difference is ≤0.001；
According to above step, 530 nm and 580nm are carried out at upper and lower each 20 mm of every tablet filter center position respectively
The measurement of positive and negative transmittance values；During 530 nm of wavelength, optical filter the position transmittance measured value of upper, middle and lower 3 difference it is exhausted
Maximum to value is the optical filter in 530 nm transmittance uniformities；During 580 nm of wavelength, optical filter is in the position of upper, middle and lower 3
The maximum of the absolute value of the difference of transmittance measured value is that the optical filter exists in 580 nm transmittance uniformities, every tablet filter
530 nm and 580 nm transmittances uniformities are ≤0.001；
（2）Spectral neutrality optical filter assembles：
Filter supporter is made, for the aluminium brackets of surface blackening processing, support body length 22 mm, wide 22 mm, high 96 mm, away from branch
There is wide 3 mm plush copper below frame upper end at 12 mm（1）, for filter supporter to be suspended on into nephelometry titer of antibodies measure
There are long 58 mm, wide 18 mm, deep 11 mm elongate recesses on the culturing rack of instrument, in support（2）, for keeping flat optical filter, groove
On have long 52 mm, wide 14 mm, both ends are semicircle, centre for square perforate（3）；
The apparent size of spring leaf is long 54.5 mm, wide 17 mm, 0.5 mm of thickness, and slotted eye is long 34 mm, and wide 14 mm, top is
There is the opening of square semicircle, bottom（5）；
1 tablet filter is lain against into the groove in 1 support（2）In, spring leaf is lain on optical filter, in the spring leaf of support
Fixing hole（4）Place is fastened spring leaf with screw, optical filter is fixed on support, is assembled into 1 spectral neutrality optical filter；
（3）, spectral neutrality optical filter absorbance standard value measurement：
With meeting《Ultraviolet, visible, near infrared spectrometer national metrological verification regulations》The spectrophotometer of I levels requirement is to every
Piece spectral neutrality optical filter measures in 530 nm and 580 nm absorbance standard value respectively；
It is 530 nm to adjust spectrophotometer measurement wavelength, the nm of spectral bandwidth 2, reference is done with air, by spectrophotometer school
Zero, it is 0.000 to make its absorbance, and 1 spectral neutrality optical filter is positioned in the light path of spectrophotometer, vertical with light path,
Light path passes through spectral neutrality optical filter front center point position, reads absorbance, and continuous measure 3 times is averaged, as should
The nm of spectral neutrality optical filter 530 front absorbance；Light path is passed through into spectral neutrality optical filter reverse side center position again,
Absorbance is read, continuous measure 3 times is averaged, and is the spectral neutrality optical filter 530 nm reverse side absorbance；Should
530 nm of spectral neutrality optical filter front absorbance and the average value of reverse side absorbance are the spectral neutrality optical filter
In 530 nm absorbance standard value, spectrophotometer measurement wavelength is set to 580 nm and carries out above measurement, calculates the spectrum
Absorbance standard value of the neutral colour filter in 580 nm；
（4）, spectral neutrality filter set matches somebody with somebody：
Every group of spectral neutrality optical filter includes 8 spectral neutrality optical filters, and the wherein absorbance trade mark is 0.3,0.7,1.0 optical filtering
Each 1 of piece, the absorbance trade mark are 0.5 optical filter 5, and the absorbance trade mark is 0.3,0.5,0.7,1.0 each 1 spectral neutrality
The error of indication that optical filter is used for nephelometry titer of antibodies analyzer absorbance measures, and the absorbance trade mark is 0.5 5 spectrum
Neutral colour filter 530 nm absorbance standard values difference should≤0.003,580 nm absorbance standard values difference≤
0.003, the measurement for nephelometry titer of antibodies analyzer interchannel absorbance difference；
（5）, the instrument absorbance error of indication measurement：
By nephelometry titer of antibodies analyzer under the conditions of unobstructed light path, using air as reference, it is by the absorbance trade mark
0.3rd, 0.5,0.7,1.0 4 spectral neutrality optical filters, which are respectively perpendicular, is placed in nephelometry titer of antibodies analyzer sample cell,
Absorbance is continuously measured per tablet filter and is averaged for 3 times and is inhaled for the optical filter absorbance measurement, 4 spectral neutrality optical filters
The maximum of the difference of photometric measurement value and standard value be the nephelometry titer of antibodies analyzer the absorbance error of indication, extinction
Spend the error of indication≤0.03；
（6）, absorbance difference measures between instrument channel：
It is 0.5 by the absorbance trade mark using air as reference by nephelometry titer of antibodies analyzer under the conditions of unobstructed light path
5 spectral neutrality optical filters be respectively perpendicular and be placed in each passage sample cell of nephelometry titer of antibodies analyzer, each passage
Continuous measurement absorbance is averaged for 3 times as the passage absorbance measurement, the maximum of each passage absorbance measurement with
The difference of minimum value be the nephelometry titer of antibodies analyzer interchannel absorbance difference, interchannel absorbance difference≤
0.03。
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