CN105606551A - Absorbance quality measurement method for nephelometry antibiotic titer tester - Google Patents
Absorbance quality measurement method for nephelometry antibiotic titer tester Download PDFInfo
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- CN105606551A CN105606551A CN201610066314.0A CN201610066314A CN105606551A CN 105606551 A CN105606551 A CN 105606551A CN 201610066314 A CN201610066314 A CN 201610066314A CN 105606551 A CN105606551 A CN 105606551A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Abstract
The invention relates to an absorbance quality measurement method for a nephelometry antibiotic titer tester. According to the absorbance quality measurement method, the indication error in the measurement of the nephelometry antibiotic titer tester and the difference among absorbances of channels can be effectively solved. The absorbance quality measurement method comprises the following steps: selecting neutral gray glass as optical filters, and respectively mounting the optical filters on an optical filter bracket, so as to assemble four spectrographic neutral optical filters; measuring an absorbance standard value of the spectrographic neutral optical filters, assembling the spectrographic neutral optical filters, measuring the absorbance indication error of the antibiotic titer tester, and measuring the difference among the absorbances of the channels of the antibiotic titer tester. The absorbance quality measurement method is stable and reliable, is high in measurement precision, is applicable to the delivery inspection of the nephelometry antibiotic titer tester and the quality monitoring of the nephelometry antibiotic titer tester by measurement technique mechanisms, and the measurement problems of the indication error in absorbance measurement results of the nephelometry antibiotic titer tester and the difference among the measurement results of the channels are effectively solved.
Description
Technical field
The present invention relates to mass measurement, particularly a kind of absorbance mass measurement side of nephelometry titer of antibodies analyzerMethod.
Background technology
" Pharmacopoeia of People's Republic of China (2015 editions) " regulation, nephelometry is the one measurement side that titer of antibodies is measuredMethod. According to pharmacopeia, relevant enterprise has developed nephelometry titer of antibodies analyzer, and this instrument is to utilize antibiotic to train liquidIn nutrient solution, test the inhibitory action of bacteria growing, by measuring respectively the fluid nutrient medium that adds standard sample and add sampleAbsorbance size, to measure the instrument of sample titer of antibodies.
It is single wavelength that nephelometry titer of antibodies analyzer is measured wavelength, according to user's needs, is equipped with 530nm or 580nmMonochromatic light light source. " spectrophotometer etalon national metrological verification regulations " requires first-order spectrum neutral colour filter:Transmittance positive and negative difference≤0.001, transmittance uniformity≤0.001. " titer of antibodies analyzer country measurement and calibration ruleModel " to the absorbance error of indication requirement of nephelometry titer of antibodies analyzer be: absorbance≤0.03, interchannel absorbance is poorDifferent requirement is: absorbance≤0.03.
At present the solwution method of absorbance mass measuring method is replaced by spectral neutrality optical filter just gradually. As light splitting lightThe absorbance mass measurement of degree meter adopts the absorbance trade mark to be respectively 0.5,0.7,1.0, and the spectral neutrality that light path is 10mm filtersSheet, the absorbance mass measurement of enzyme micro-plate reader adopts the absorbance trade mark to be respectively 0.2,0.5,1.0,1.5, and light path is 10mmSpectral neutrality optical filter. But the light path of nephelometry titer of antibodies analyzer is 20mm, above spectral neutrality optical filter is inapplicableIn the absorbance mass measurement of nephelometry titer of antibodies analyzer.
The absorbance mass measuring method of existing nephelometry titer of antibodies analyzer adopts solwution method more, molten by sameLiquid (as cobalt chloride solution) joins in the cuvette of nephelometry titer of antibodies analyzer, respectively at nephelometry titer of antibodiesOn the different passages of analyzer, carry out absorbance measurement, to control different interchannel othernesses. Solwution method is uncontrollable everyThe absorbance error of indication of individual passage, the unholiness property of the inhomogeneities of solution and cuvette also can have influence on absorbance matter in additionMeasurement amount. Therefore,, for guaranteeing the quality of nephelometry titer of antibodies analyzer absorbance measuring result, need to develop new turbidityThe absorbance mass measuring method of method titer of antibodies analyzer.
Summary of the invention
For above-mentioned situation, for overcoming the defect of prior art, the present invention's object is just to provide a kind of nephelometry antibiosisThe absorbance mass measuring method of element titration instrument, can effectively solve nephelometry titer of antibodies analyzer absorbance measuringThe variability issues of absorbance between the error of indication, each passage.
The technical scheme that the present invention solves is to be realized by following steps:
(1), select optical filter:
First select 4 kinds of neutral gray glass to make optical filter, the glass trade mark be respectively ZAB50, ZAB30, ZAB20,ZAB10, the corresponding absorbance trade mark is respectively 0.3,0.5,0.7,1.0, and every kind of optical filtering length of a film is 56-60mm, wide 16-20mm, thick 1.8-2.2mm, transmittance positive and negative difference≤0.001, transmittance uniformity≤0.001;
(2), assembling spectral neutrality optical filter:
The optical filter that 4 kinds of neutral gray glass are made is contained in respectively in filter supporter, and filter supporter is aluminum, tableFace blackout, stent size size is identical with the cuvette of nephelometry titer of antibodies analyzer, has perforate on support, guarantees turbidityThe light that method titer of antibodies analyzer sends by support, lies against respectively opening of 4 supports by 4 kinds of different optical filters completelyHole place, with the fixing optical filter of the spring leaf not being in the light, is assembled into 4 kinds of spectral neutrality optical filters;
Described filter supporter is to be made up of the support body 6 of aluminum, and support body 6 both sides, top are useful on filter supporter outstandingHang over the plush copper 1 on the culturing rack of nephelometry titer of antibodies analyzer, in support body, be useful on the elongate recesses that keeps flat optical filter2, in the middle of groove, be provided with foursquare perforate 3, on the support body on groove 2 tops, there is spring leaf fixing hole 4, there is opening spring leaf bottom5, the fixing hole corresponding with spring leaf fixing hole 49 arranged at spring leaf 7 tops, and bottom has and the consistent corresponding slotted eye of perforate 38, optical filter lies against in groove 2, and spring leaf 7 is pressed in above optical filter, spring leaf through fixing hole 9, spring leaf fixing hole 4 by spiral shellNail is fixed on spring leaf on groove 2, and filter set is contained on support, forms a slice spectral neutrality filter sheet structure;
(3), measure spectrum neutral colour filter absorbance standard value:
4 kinds of spectral neutrality optical filters are carried out to absorbance measurement at 530nm and 580nm respectively with spectrophotometer,The measured value of 530nm is this spectral neutrality optical filter 530nm absorbance standard value, is this spectral neutrality at the measured value of 580nmOptical filter 580nm absorbance standard value;
(4), assembly spectral neutrality optical filter:
Each spectral neutrality filter set comprises 8 spectral neutrality optical filters: the ZAB50 neutrality that the absorbance trade mark is 0.3ZAB20 neutral gray glass optical filter, the absorbance trade mark that smoked glass optical filter, the absorbance trade mark are 0.7 are 1.0Each 1 of ZAB100 neutral gray glass optical filter, 5 of the ZAB30 neutral gray glass optical filters that the absorbance trade mark is 0.5, inhaleThe luminosity trade mark is that 0.3 ZAB50 neutral gray glass optical filter, the ZAB30 neutral gray glass that the absorbance trade mark is 0.5 filterThe ZAB20 neutral gray glass optical filter that sheet, the absorbance trade mark are 0.7, the ZAB10 neutral gray glass that the absorbance trade mark is 1.0Each 1 of glass optical filter, for the measurement of the nephelometry titer of antibodies analyzer absorbance error of indication; The absorbance trade mark is 0.55 of ZAB30 neutral gray glass optical filters, for the survey of nephelometry titer of antibodies analyzer interchannel absorbance differenceAmount;
(5), the titer of antibodies analyzer absorbance error of indication is measured:
The ZAB50 neutral gray glass optical filter that is 0.3 by the absorbance trade mark, the ZAB30 neutrality that the absorbance trade mark is 0.5ZAB20 neutral gray glass optical filter, the absorbance trade mark that smoked glass optical filter, the absorbance trade mark are 0.7 are 1.04 spectral neutrality optical filters of ZAB100 neutral gray glass optical filter are vertically placed in respectively nephelometry titer of antibodies analyzerIn sample cell, measure, the maximum of the difference of 4 spectral neutrality optical filter absorbance measuring values and standard value is that nephelometry is anti-The raw plain titration instrument absorbance error of indication, the absorbance error of indication≤0.03;
(6), titer of antibodies analyzer interchannel absorbance difference is measured:
The ZAB30 neutral gray glass optical filter that is 0.5 by 5 absorbance trades mark is vertically placed in respectively nephelometry antibioticIn the each passage sample cell of titration instrument, measure, 5 spectral neutrality optical filters are in the maximum of each passage absorbance measuring valueThe difference of value and minimum of a value is interchannel absorbance difference, interchannel absorbance difference≤0.03.
The inventive method is reliable and stable, and certainty of measurement is high, efficiently solves nephelometry titer of antibodies analyzer absorbanceThe error of indication of measurement result is measured and the otherness of each interchannel measurement result is measured a difficult problem, is not only suitable for nephelometry antibiosisThe exfactory inspection of element titration instrument, is also applicable to the quality prison of measurement technology mechanism to nephelometry titer of antibodies analyzerSurvey, have actual using value, economic and social profit is remarkable.
Brief description of the drawings
Fig. 1 is filter supporter structural front view of the present invention.
Fig. 2 is filter supporter profile of the present invention.
Fig. 3 is filter supporter structure upward view of the present invention.
Fig. 4 is filter supporter chip architecture of the spring front view of the present invention.
Detailed description of the invention
Elaborate below in conjunction with concrete condition the specific embodiment of the present invention.
The present invention, in concrete enforcement, can be realized by following steps:
(1) optical filter is selected:
Optical filter is 4 kinds of neutral gray glass, and the glass trade mark is respectively ZAB50, ZAB30, ZAB20, ZAB10, correspondingThe absorbance trade mark is respectively 0.3,0.5,0.7,1.0, and every kind of optical filter is thick 2mm, long 58mm, wide 18mm;
With the spectrophotometer that meets " ultraviolet, visible, near infrared spectrometer national metrological verification regulations " I level requirementEvery tablet filter is measured in transmittance positive and negative difference, the transmittance uniformity of 530nm and 580nm respectively;
First spectrophotometer measurement wavelength is made as to 530nm, spectral bandwidth 2nm, does reference with air, by spectrophotometerSchool zero, making its transmittance is 1.000, and 1 tablet filter is positioned in light path, vertical with light path, light path is mating plate front after filtrationCenter position, reads transmission ratio, and continuous measurement is averaged for 3 times, is the positive transmission ratio of this optical filter 530nm,By light path mating plate reverse side center position after filtration, read transmission ratio again, continuous measurement is averaged for 3 times, is this optical filteringThe reverse side transmission ratio of sheet 530nm, the absolute value of the positive transmission ratio of this optical filter 530nm and the difference of reverse side transmission ratio isThis optical filter, in the transmittance positive and negative difference of 530nm, is made as 580nm by spectrophotometer measurement wavelength and measures, and calculatesThis optical filter is in the transmittance positive and negative difference of 580nm, and every tablet filter is in the transmittance positive and negative difference of 530nm and 580nmAll≤0.001;
According to above step, carry out at upper and lower each 20mm place of every tablet filter center position respectively 530nm andThe positive and negative transmission ratio of 580nm is measured; When wavelength 530nm, optical filter is poor upper, middle and lower 3 position transmittance measured valuesThe maximum of absolute value be that this optical filter is in 530nm transmittance uniformity; When wavelength 580nm, 3 in upper, middle and lower of optical filtersPut the difference of transmittance measured value absolute value maximum for this optical filter in 580nm transmittance uniformity, every tablet filter exists530nm and 580nm transmittance uniformity are all≤0.001;
(2) spectral neutrality optical filter assembling:
Making filter supporter, is the aluminium brackets (seeing Fig. 1) of surface blackening processing, the long 22mm of support body, wide 22mm, height96mm, has the plush copper 1 of wide 3mm apart from 12mm place, support upper end below, imitate for filter supporter being suspended on to nephelometry antibioticOn the culturing rack of valency analyzer, in support, there is long 58mm, wide 18mm, the elongate recesses 2 of dark 11mm is for keeping flat optical filter, recessedOn groove, there is long 52mm, wide 14mm, two ends are semicircle, centre is foursquare perforate 3;
The apparent size of spring leaf (as Fig. 4) is long 54.5mm, wide 17mm, and thick 0.5mm, slotted eye is long 34mm, wide 14mm,Top is semicircle, and there is foursquare opening 5 bottom;
1 tablet filter is lain against in 1 groove 2 in support, and spring leaf lies on optical filter, at the spring of supportSheet fixing hole 4 places are fastening by spring leaf with screw, and optical filter is fixed on support, are assembled into 1 spectral neutrality optical filter (4The assemble method of planting optical filter is identical, does not describe one by one);
(3), spectral neutrality optical filter absorbance standard value is measured:
With the spectrophotometer that meets " ultraviolet, visible, near infrared spectrometer national metrological verification regulations " I level requirementEvery spectral neutrality optical filter is measured in the absorbance standard value of 530nm and 580nm respectively;
Adjusting spectrophotometer measurement wavelength is 530nm, and spectral bandwidth 2nm, does reference with air, by spectrophotometer schoolZero, making its absorbance is 0.000, and 1 spectral neutrality optical filter is positioned in spectrophotometric light path, vertical with light path,Light path, through spectral neutrality optical filter front center point position, is read absorbance, and continuous measurement is averaged for 3 times, is thisThe front absorbance of spectral neutrality optical filter 530nm; By light path process spectral neutrality optical filter reverse side center position, read againGo out absorbance, continuous measurement is averaged for 3 times, is the reverse side absorbance of this spectral neutrality optical filter 530nm; This spectrumThe front absorbance of the 530nm of neutral colour filter and the mean value of reverse side absorbance are that this spectral neutrality optical filter existsThe absorbance standard value of 530nm, is made as 580nm by spectrophotometer measurement wavelength and carries out above measurement, calculates this spectral neutralityOptical filter is in the absorbance standard value of 580nm;
(4), spectral neutrality optical filter assembly:
Every group of spectral neutrality optical filter comprise 8 spectral neutrality optical filters, and wherein the absorbance trade mark is 0.3,0.7,1.0Each 1 of optical filter, 5 of the optical filters that the absorbance trade mark is 0.5, the absorbance trade mark is each 1 spectrum of 0.3,0.5,0.7,1.0Neutral colour filter is measured for the error of indication of nephelometry titer of antibodies analyzer absorbance, the absorbance trade mark is 0.5 5Spectral neutrality optical filter should≤0.003 in the difference of 530nm absorbance standard value, the difference of 580nm absorbance standard value≤0.003, for the measurement of nephelometry titer of antibodies analyzer interchannel absorbance difference;
(5), the instrument absorbance error of indication is measured:
By nephelometry titer of antibodies analyzer under unobstructed light path condition, taking air as reference, by the absorbance trade markBe that 4 spectral neutrality optical filters of 0.3,0.5,0.7,1.0 are vertically placed in respectively nephelometry titer of antibodies analyzer sample cellIn, every tablet filter continuous measurement absorbance is averaged for 3 times as this optical filter absorbance measuring value, and 4 spectral neutralities filterThe maximum of the difference of sheet absorbance measuring value and standard value is the absorbance error of indication of this nephelometry titer of antibodies analyzer,The absorbance error of indication≤0.03;
(6), between instrument channel, absorbance difference is measured:
By nephelometry titer of antibodies analyzer under unobstructed light path condition, taking air as reference, by the absorbance trade markBe that 5 spectral neutrality optical filters of 0.5 are vertically placed in respectively the each passage sample cell of nephelometry titer of antibodies analyzer, eachPassage continuous measurement absorbance is averaged for 3 times as this passage absorbance measuring value, the maximum of each passage absorbance measuring valueThe difference of value and minimum of a value is the interchannel absorbance difference of this nephelometry titer of antibodies analyzer, interchannel absorbance difference≤0.03。
The present invention, through application on the spot and calculating, has obtained very satisfied useful technique effect, and interrelated data is as follows:
The present invention is through application on the spot and test described optical filter:
The neutral gray glass of selecting is that the glass trade mark that Yongxing, Beijing perception information Technology Co., Ltd. produces is4 kinds of neutral gray glass of ZAB50, ZAB30, ZAB20, ZAB10, thick 2mm, the corresponding absorbance trade mark is respectively 0.3,0.5,0.7,1.0, every kind of neutral gray glass absorption spectrum within the scope of wavelength 500nm～600nm is smoothed curve. By every kindNeutral gray glass cut growth 58mm, the optical filter of wide 18mm. With I level Cary5000 type ultraviolet, visible, near-infrared light splittingSpectrophotometer is measured the transmission ratio of each optical filter, and the maximum of transmittance positive and negative difference is 0.0007, transmissionBe 0.0008 than the maximum of uniformity difference, meet the requirement of first-order spectrum neutral colour filter;
Described spectral neutrality optical filter assembling: make filter supporter, support is aluminum, surface blackening, stent sizeSize is identical with nephelometry titer of antibodies analyzer cuvette, has perforate on support, guarantees nephelometry titer of antibodies mensurationThe light that instrument light source sends passes through support completely; Make spring leaf, for optical filter is fixed on to filter supporter; By 4 kinds notSame optical filter lies against respectively on the trough of 4 supports, fixes with spring leaf, and spring leaf is not in the light, and is assembled in 4 kinds of spectrumProperty optical filter.
Described spectral neutrality optical filter absorbance standard value is measured:
With I level Cary5000 type ultraviolet, visible, near-infrared light splitting spectrophotometer respectively at 530nm and 580nm to respectivelySpectral neutrality optical filter absorbance standard value is measured, and measurement result sees the following form;
Described spectral neutrality optical filter assembly: each spectral neutrality filter set comprises 8 spectral neutrality optical filters, itsThe middle absorbance trade mark is each 1 of 0.3,0.7,1.0 spectral neutrality optical filter, and the spectral neutrality that the absorbance trade mark is 0.5 filters5 of sheets, 5 spectral neutrality optical filters that in this group, the absorbance trade mark is 0.5 in the difference of 530nm absorbance standard value are0.001, be 0.002 (seeing the above table) in the difference of 580nm absorbance standard value, be all less than 0.003, meet the requirements.
The described nephelometry titer of antibodies analyzer absorbance error of indication is measured:
By certain nephelometry titer of antibodies analyzer under unobstructed light path condition, taking air as reference, by absorbanceThe trade mark is that 4 spectral neutrality optical filters of 0.3,0.5,0.7,1.0 are vertically placed in respectively this nephelometry titer of antibodies and measureIn instrument sample cell, every tablet filter continuous measurement is averaged for 3 times as this optical filter absorbance measuring value, this nephelometry antibiosisElement titration instrument sees the following form to 4 spectral neutrality optical filter absorbance measuring values. Nephelometry titer of antibodies analyzer is to 4The maximum of the difference of spectral neutrality optical filter absorbance measuring value and spectral neutrality optical filter absorbance standard value is this turbidityThe method titer of antibodies analyzer absorbance error of indication, the nephelometry titer of antibodies analyzer absorbance error of indication is-0.018, be less than 0.03, show that absorbance Measurement Result meets the requirements;
Between described instrument channel, absorbance difference is measured:
By certain nephelometry titer of antibodies analyzer under unobstructed light path condition, taking air as reference, by absorbanceThe trade mark is that 5 spectral neutrality optical filters of 0.5 are vertically placed in respectively the each passage sample of this nephelometry titer of antibodies analyzerIn groove, each passage continuous measurement is averaged for 3 times as this passage absorbance measuring value of instrument, each passage absorbance measuring valueMaximum and the difference of minimum of a value be absorbance difference between instrument channel, the each passage of this nephelometry titer of antibodies analyzerAbsorbance measuring value sees the following form, and interchannel absorbance difference is 0.008, is less than 0.03, shows that between this instrument channel, absorbance is poorDifferent measurement result meets the requirements.
From above-mentioned situation, the present invention by optical filter select, spectral neutrality optical filter assembling, spectral neutrality optical filterBetween the measurement of absorbance standard value, the assembly of spectral neutrality optical filter, the measurement of the instrument absorbance error of indication, instrument channel, absorbance is poorDifferent measurement, adopts the glass trade mark to be respectively 4 kinds of neutral gray glass of ZAB50, ZAB30, ZAB20, ZAB10, is made into spectrumNeutral colour filter group, has effectively solved nephelometry titer of antibodies analyzer absorbance indicating value mistake by this invention of examples proveThe poor difficult problem such as measurement and the measurement of the each interchannel absorbance of nephelometry titer of antibodies analyzer difference, this invention is not only suitableFor the exfactory inspection of nephelometry titer of antibodies analyzer, be also applicable to measurement technology mechanism nephelometry titer of antibodies is surveyedDetermine the quality-monitoring of instrument, have actual using value, economic and social profit is remarkable.
Claims (3)
1. an absorbance mass measuring method for nephelometry titer of antibodies analyzer, is characterized in that, real by following stepsExisting:
(1), select optical filter:
First select 4 kinds of neutral gray glass to make optical filter, the glass trade mark is respectively ZAB50, ZAB30, ZAB20, ZAB10,The corresponding absorbance trade mark is respectively 0.3,0.5,0.7,1.0, and every kind of optical filtering length of a film is 56-60mm, wide 16-20mm, thick1.8-2.2mm, transmittance positive and negative difference≤0.001, transmittance uniformity≤0.001;
(2), assembling spectral neutrality optical filter:
The optical filter that 4 kinds of neutral gray glass are made is contained in respectively in filter supporter, and filter supporter is that aluminum, surface are sent outBlack, stent size size is identical with the cuvette of nephelometry titer of antibodies analyzer, has perforate on support, guarantees that nephelometry is anti-The light that raw plain titration instrument sends, completely by support, lies against 4 kinds of different optical filters respectively the perforate of 4 supportsPlace, with the fixing optical filter of the spring leaf not being in the light, is assembled into 4 kinds of spectral neutrality optical filters;
(3), measure spectrum neutral colour filter absorbance standard value:
4 kinds of spectral neutrality optical filters are carried out to absorbance measurement at 530nm and 580nm respectively with spectrophotometer, 530The measured value of nm is this spectral neutrality optical filter 530nm absorbance standard value, is this spectral neutrality filter at the measured value of 580nmMating plate 580nm absorbance standard value;
(4), assembly spectral neutrality optical filter:
Each spectral neutrality filter set comprises 8 spectral neutrality optical filters: the ZAB50 neutral gray that the absorbance trade mark is 0.3In the ZAB20 neutral gray glass optical filter that glass filter, the absorbance trade mark are 0.7, the ZAB100 that the absorbance trade mark is 1.0Each 1 of property smoked glass optical filter, 5 of the ZAB30 neutral gray glass optical filters that the absorbance trade mark is 0.5, the absorbance trade markBe 0.3 ZAB50 neutral gray glass optical filter, ZAB30 neutral gray glass optical filter, the extinction that the absorbance trade mark is 0.5The ZAB20 neutral gray glass optical filter that the degree trade mark is 0.7, the ZAB10 neutral gray glass optical filter that the absorbance trade mark is 1.0Each 1, for the measurement of the nephelometry titer of antibodies analyzer absorbance error of indication; The absorbance trade mark is in 0.5 ZAB305 of property smoked glass optical filters, for the measurement of nephelometry titer of antibodies analyzer interchannel absorbance difference;
(5), the titer of antibodies analyzer absorbance error of indication is measured:
The ZAB50 neutral gray glass optical filter that is 0.3 by the absorbance trade mark, the ZAB30 neutral gray that the absorbance trade mark is 0.5In the ZAB20 neutral gray glass optical filter that glass filter, the absorbance trade mark are 0.7, the ZAB100 that the absorbance trade mark is 1.04 spectral neutrality optical filters of property smoked glass optical filter are vertically placed in respectively nephelometry titer of antibodies analyzer sample cellMeasure, the maximum of the difference of 4 spectral neutrality optical filter absorbance measuring values and standard value is nephelometry titer of antibodiesThe analyzer absorbance error of indication, the absorbance error of indication≤0.03;
(6), titer of antibodies analyzer interchannel absorbance difference is measured:
The ZAB30 neutral gray glass optical filter that is 0.5 by 5 absorbance trades mark is vertically placed in respectively nephelometry titer of antibodiesIn the each passage sample cell of analyzer, measure, 5 spectral neutrality optical filters the maximum of each passage absorbance measuring value withThe difference of minimum of a value is interchannel absorbance difference, interchannel absorbance difference≤0.03.
2. the absorbance mass measuring method of nephelometry titer of antibodies analyzer according to claim 1, its feature existsIn, described filter supporter is to be made up of the support body of aluminum (6), support body (6) both sides, top are useful on filter supporter outstandingHang over the plush copper (1) on the culturing rack of nephelometry titer of antibodies analyzer, in support body, be useful on and keep flat the microscler recessed of optical filterGroove (2), is provided with foursquare perforate (3) in the middle of groove, on the support body on groove (2) top, has spring leaf fixing hole (4), spring leafThere is opening (5) bottom, and the fixing hole (9) corresponding with spring leaf fixing hole (4) arranged at spring leaf (7) top, and bottom has and opensThe slotted eye (8) of the consistent correspondence in hole (3), optical filter lies against in groove (2), and spring leaf (7) is pressed in above optical filter, spring leafBy screw, spring leaf is fixed on to groove (2) above through fixing hole (9), spring leaf fixing hole (4), makes filter set be contained in supportUpper, form a slice spectral neutrality filter sheet structure.
3. the absorbance mass measuring method of nephelometry titer of antibodies analyzer according to claim 1, its feature existsIn, realized by following steps:
(1) optical filter is selected:
Optical filter is 4 kinds of neutral gray glass, and the glass trade mark is respectively ZAB50, ZAB30, ZAB20, ZAB10, corresponding extinctionThe degree trade mark is respectively 0.3,0.5,0.7,1.0, and every kind of optical filter is thick 2mm, long 58mm, wide 18mm;
The spectrophotometer that " ultraviolet, visible, near infrared spectrometer national metrological verification regulations " I level requires with meeting is to oftenTablet filter is measured in transmittance positive and negative difference, the transmittance uniformity of 530nm and 580nm respectively;
First spectrophotometer measurement wavelength is made as to 530nm, spectral bandwidth 2nm, does reference with air, by spectrophotometer schoolZero, making its transmittance is 1.000, and 1 tablet filter is positioned in light path, vertical with light path, and light path is after filtration in mating plate frontHeart point position, reads transmission ratio, and continuous measurement is averaged for 3 times, is the positive transmission ratio of this optical filter 530nm, thenBy light path mating plate reverse side center position after filtration, read transmission ratio, continuous measurement is averaged for 3 times, is this optical filterThe reverse side transmission ratio of 530nm, the absolute value of the positive transmission ratio of this optical filter 530nm and the difference of reverse side transmission ratio isThis optical filter, in the transmittance positive and negative difference of 530nm, is made as 580nm by spectrophotometer measurement wavelength and measures, meterCalculate the transmittance positive and negative difference of this optical filter at 580nm, every tablet filter is at the transmittance positive and negative of 530nm and 580nmDifference all≤0.001;
According to above step, carry out 530nm and 580nm at upper and lower each 20mm place of every tablet filter center position respectivelyPositive and negative transmission ratio measure; When wavelength 530nm, optical filter the difference of upper, middle and lower 3 position transmittance measured values absolutelyMaximum to value is that this optical filter is in 530nm transmittance uniformity; When wavelength 580nm, optical filter is in 3 positions, upper, middle and lowerThe maximum of the absolute value of the difference of transmittance measured value be this optical filter in 580nm transmittance uniformity, every tablet filter exists530nm and 580nm transmittance uniformity are all≤0.001;
(2) spectral neutrality optical filter assembling:
Making filter supporter, is the aluminium brackets of surface blackening processing, the long 22mm of support body, and wide 22mm, high 96mm, apart from propping upThere is the plush copper (1) of wide 3mm at 12mm place, below, frame upper end, measures for filter supporter being suspended on to nephelometry titer of antibodiesOn the culturing rack of instrument, in support, there is long 58mm, wide 18mm, the elongate recesses (2) of dark 11mm, for keeping flat optical filter, grooveOn have long 52mm, wide 14mm, two ends are semicircle, centre is foursquare perforate (3);
The apparent size of spring leaf is long 54.5mm, wide 17mm, and thick 0.5mm, slotted eye is long 34mm, wide 14mm, top isSemicircle, there is foursquare opening (5) bottom;
1 tablet filter is lain against in 1 groove (2) in support, and spring leaf lies on optical filter, at the spring leaf of supportFixing hole (4) is located with screw spring leaf is fastening, and optical filter is fixed on support, is assembled into 1 spectral neutrality optical filter;
(3), spectral neutrality optical filter absorbance standard value is measured:
The spectrophotometer that " ultraviolet, visible, near infrared spectrometer national metrological verification regulations " I level requires with meeting is to oftenSheet spectral neutrality optical filter is measured in the absorbance standard value of 530nm and 580nm respectively;
Adjusting spectrophotometer measurement wavelength is 530nm, and spectral bandwidth 2nm, does reference with air, by spectrophotometer schoolZero, making its absorbance is 0.000, and 1 spectral neutrality optical filter is positioned in spectrophotometric light path, vertical with light path,Light path, through spectral neutrality optical filter front center point position, is read absorbance, and continuous measurement is averaged for 3 times, is thisThe front absorbance of spectral neutrality optical filter 530nm; Again light path is passed through to spectral neutrality optical filter reverse side center position,Read absorbance, continuous measurement is averaged for 3 times, is the reverse side absorbance of this spectral neutrality optical filter 530nm; ShouldThe front absorbance of the 530nm of spectral neutrality optical filter and the mean value of reverse side absorbance are this spectral neutrality optical filterIn the absorbance standard value of 530nm, spectrophotometer measurement wavelength is made as to 580nm and carries out above measurement, calculate this spectrumNeutral colour filter is in the absorbance standard value of 580nm;
(4), spectral neutrality optical filter assembly:
Every group of spectral neutrality optical filter comprises 8 spectral neutrality optical filters, and wherein the absorbance trade mark is 0.3,0.7,1.0 optical filteringEach 1 of sheet, 5 of the optical filters that the absorbance trade mark is 0.5, the absorbance trade mark is each 1 spectral neutrality of 0.3,0.5,0.7,1.0Optical filter is measured for the error of indication of nephelometry titer of antibodies analyzer absorbance, 5 spectrum that the absorbance trade mark is 0.5Neutral colour filter should≤0.003 in the difference of 530nm absorbance standard value, the difference of 580nm absorbance standard value≤0.003, for the measurement of nephelometry titer of antibodies analyzer interchannel absorbance difference;
(5), the instrument absorbance error of indication is measured:
By nephelometry titer of antibodies analyzer under unobstructed light path condition, taking air as reference, by the absorbance trade mark be0.3,0.5,0.7,1.0 4 spectral neutrality optical filters are vertically placed in respectively nephelometry titer of antibodies analyzer sample cell,Every tablet filter continuous measurement absorbance is averaged for 3 times as this optical filter absorbance measuring value, and 4 spectral neutrality optical filters are inhaledThe maximum of the difference of photometric measurement value and standard value is the absorbance error of indication of this nephelometry titer of antibodies analyzer, extinctionThe degree error of indication≤0.03;
(6), between instrument channel, absorbance difference is measured:
Under unobstructed light path condition, taking air as reference, be 0.5 by the absorbance trade mark by nephelometry titer of antibodies analyzer5 spectral neutrality optical filters be vertically placed in respectively the each passage sample cell of nephelometry titer of antibodies analyzer, each passageContinuous measurement absorbance is averaged for 3 times as this passage absorbance measuring value, the maximum of each passage absorbance measuring value withThe difference of minimum of a value is the interchannel absorbance difference of this nephelometry titer of antibodies analyzer, interchannel absorbance difference≤0.03。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109612956A (en) * | 2019-01-08 | 2019-04-12 | 杭州霆科生物科技有限公司 | A kind of optical correction's chip for the residual detection system of agriculture |
| CN111562204A (en) * | 2020-03-03 | 2020-08-21 | 河南省计量科学研究院 | Calibration method of light-tight smoke meter optical filter |
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| CN102297931A (en) * | 2011-05-20 | 2011-12-28 | 浙江大学 | Antibiotic titer determination method based on ink-jet printing principle |
| CN103913426A (en) * | 2013-01-08 | 2014-07-09 | 中国科学院生态环境研究中心 | Method for evaluation of residual biopotency of antibiotics in waste water |
| WO2015128650A1 (en) * | 2014-02-26 | 2015-09-03 | Spectromics Limited | A method of analysing a sample including a microorganism of interest |
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| CN102297931A (en) * | 2011-05-20 | 2011-12-28 | 浙江大学 | Antibiotic titer determination method based on ink-jet printing principle |
| CN103913426A (en) * | 2013-01-08 | 2014-07-09 | 中国科学院生态环境研究中心 | Method for evaluation of residual biopotency of antibiotics in waste water |
| WO2015128650A1 (en) * | 2014-02-26 | 2015-09-03 | Spectromics Limited | A method of analysing a sample including a microorganism of interest |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109612956A (en) * | 2019-01-08 | 2019-04-12 | 杭州霆科生物科技有限公司 | A kind of optical correction's chip for the residual detection system of agriculture |
| CN111562204A (en) * | 2020-03-03 | 2020-08-21 | 河南省计量科学研究院 | Calibration method of light-tight smoke meter optical filter |
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