CN206613510U - Suction pipette head device and its measuring system - Google Patents

Abstract

Present disclosure is related to can particularly promote user in the exemplary embodiment with the sample waste for reducing (minimum) and the absorbance or the system of fluorescence measurement, method, technology, process, product and the product component that are improved in the spectral range of increased yield in bioscience research.There is provided a kind of suction pipette head measuring system, it has：Base unit, it has the device for being used for positioning suction pipette head；Suction pipette head, it is designed in order to which accurate suction pipette head is positioned and is interacted with base unit；At least one light feeding unit, its fluid sample being oriented into the suction pipette head provides light；And at least one light collector unit, it is oriented to the light for collecting the fluid sample in the suction pipette head.

Description

Suction pipette head device and its measuring system

Technical field

Present disclosure is related to can particularly promote user with the sample for reducing (minimum) in bioscience research The absorbance or system, method, technology, the mistake of fluorescence measurement improved in the spectral range of product waste and increased yield The exemplary embodiment of journey, product and product component, accurate absorbance/fluorescence measurement of its target especially to fluid sample There is provided a kind of unique, effective solution, and reduce plant maintenance requirement.

Background technology

Description of related art

For routine, there is two kinds of main methods, technology or the process of measurement and analysis fluid sample.

The first conventional method is directed to use with cuvette.Cuvette is generally circular or square cross section tubule, It seals and forms the (realization for that can be directed to use with ultraviolet (UV) light by plastic material, glass or vitreous silica at one end For).Cuvette, which is designed to accommodate, is used for the sample of spectrum experiment and analysis.Cuvette is formed with specific transversal Face length --- being usually 10mm --- is to allow to readily calculate illumination level and/or absorption coefficient.

Cuvette is filled with fluid sample, and the light irradiation from specific light source is by fluid sample, from specific light The light in source is generally especially adjusted by a series of intervening optical structures, and the light that the intervening optical structures are located at cuvette is supplied Should or light incident side on and be correspondingly situated at the light of cuvette collect or reclaim side on.Involved optical element can include example Such as integrating sphere (integrating sphere), the purpose is to by fluid sample and will collect group by light from light provisioning component The light that part is reclaimed is normalized (normalize).Then, light is typically communicated to by the light of the collection of fluid sample Spectrometer, to assess absorption of the fluid sample to light, i.e. in the intensity of the light of various collected at wavelengths.

Cuvette is usually not considered as disposable, it is therefore necessary to it is carried out between sample measurement thoroughly clear It is clean, to avoid contaminated samples from measuring.In addition, wave is tended in the processing that cuvette and the fluid sample using these cuvettes are measured Take substantial amounts of particular liquid sample.

Second method is related to by making microlitre volume liquid sample between two structures (anvil (anvil)) surface Surface tension to carry out spectral measurement to fluid sample come the processing kept.Anvil surface is press polished, and generally wrapped Include the embedded fiber with the end flushed with anvil surface.Generally, one of anvil is fixed, and another anvil is Movably, to accurately control the distance between anvil, the light absorbed by fluid sample can be measured over this distance.2008 The Robertson that on July 8, authorizes et al. United States Patent (USP) No.7,397,036 describe such exemplary measurement devices and Method.Fluid sample is deposited in small footprint (pedestal).Then the fluid sample deposited is engaged and substantially by anvil It is drawn as the fluid column being supported on by the surface tension in fluid sample between anvil surface.

The element that this method still needs measurement apparatus is contacted with fluid sample, and it is also required between sample is measured to dress Put surface and carry out specific cleaning, to avoid polluting subsequent fluid sample so as to cause potential mistake to measure.This cleaning must Must carefully it carry out, so as not to influence anvil in the way of it may negatively affect fluid sample adhesion retentivity and/or optical analysis The cleaning of platform and built-in optical element and polishing property.

The content of the invention

As will be specifically described in detail below, the disclosed embodiments are related to a kind of unique suction pipette head (pipette tip) product, it can solve certain in above-mentioned conventional system according to one or more of following functions target It is a little not enough.

A kind of unique suction pipette head can be provided according to the exemplary embodiment of the system and method for present disclosure Product, the quantization of the concentration for realizing the solid constituent in the solution in fluid sample.In embodiment, solid constituent can be with Including the biological specimen in fluid sample, such as protein and nucleic acid.

Exemplary embodiment can provide the suction pipette head being inserted into suction pipette head measuring system, and pipettor is inhaled Head measuring system preferably has the feature for positioning, and is properly located suction pipette head to support fluid sample Analysis.In embodiment, suction pipette head, which can have or be arranged in, has specific cross section on light transverse direction Length, to promote the spectrum analysis of the fluid sample in the suction pipette head of certain cross section length.

In embodiment, suction pipette head can appointing by the suction nozzle in suction pipette head measuring system structure or pipettor What part is kept, and is suitably positioned between the measurement parts of structure.

In embodiment, when suction pipette head be appropriately positioned in suction pipette head measuring system structure measurement parts it Between when, suction pipette head can from pipettor eject or keep connection.

In embodiment, suction pipette head can be that transfer (transfer), ball-type (bulb) or other single-piece liquid are received The integration section of acquisition means.

Exemplary embodiment can provide a kind of light source of the specific cooperation with particular optical device, the particular optical Device is included for producing light and by the light incident side of its light projection to suction pipette head, and by fluid sample with Collected on the recovery side in suction pipette head by light collection device.In embodiment, light source can include deuterium flash lamp (deuterium flash), xenon flash lamp (xenon flash lamp), light emitting diode (LED) or other appropriate similar One or more of light source.The light of generation can generally be supplied to optical fiber cable, light pipe or other light carrying/conveyings by light source One or more of medium.The combination of these features may be generally referred to as " light feeding unit " in this disclosure.

Exemplary embodiment, which can provide the light launched by light feeding unit, to include fluid sample through disclosed Suction pipette head, and by " light collector unit " collect.Light collector unit can include the second optical fiber cable, light pipe or other One or more of light carrying/pumped (conveying) medium.

In embodiment, one or two in light feeding unit and light collector unit can also include some pH effects Component, including but not limited between light feeding unit and suction pipette head and/or between suction pipette head and light collector unit One or more lens, speculum, window and/or filter.

In embodiment, light feeding unit and light collector unit can include multiple cooperation output ports and input respectively Mouthful.This multiple cooperation output ports and input port can generally be disposed in commonly known as multiplexer (multiplexer) in structure.

In embodiment, light feeding unit and light collector unit can be that manually or automatically operate relative to each other can One or two mobile, so as to one or two in light feeding unit and light collector unit can move closer to it is disclosed Suction pipette head or move further away from disclosed suction pipette head, it can be used for or cannot be used for positioning pipettor Suction nozzle and/or optics.

In embodiment, suction pipette head and/or pipettor can manually or automatically operate movement, to measure Region is aligned with light supply/collector unit.

Exemplary embodiment can be provided：Light collector unit is by the collection for the fluid sample having already passed through in suction pipette head Light be delivered to spectrometer or be similarly used for the detector of luminous intensity/absorptiometry.Obtained measurement result can be via example Such as processing unit with one or more reference values available for the concentration that fluid sample is calculated according to any means known and technology It is related.

Exemplary embodiment, which can be provided, reclaims the ability with the suction pipette head of fluid sample internally, so as to allow Fluid sample does not have cross pollution, realizes due to minimum (there is no) sample loss caused by measurement, and substantially disappear Any demand except cleaning sample measurement surface between being measured in sample, i.e. significant to reduce the consumption between sample measurement When maintenance needs.

In one embodiment, this disclosure provides a kind of base unit by being configured to receive suction pipette head The suction pipette head device of composition, the base unit is configured to offer suction pipette head and supplied in the light for being installed to base unit Structure alignment between unit and light collector unit, wherein the base unit has the in-profile for limiting receiving space (inner profile), and the in-profile of wherein described base unit is configured to and is configured to fix pipettor suction The exterior contour of the assembly unit of head physically interacts.

In another embodiment, the invention provides a kind of suction pipette head measuring system, it is made up of following part： Base unit, the base unit has the in-profile for limiting receiving space；Assembly unit, the assembly unit is configured to Fixed suction pipette head, and the assembly unit have be configured to it is physically mutual with the in-profile of the base unit The exterior contour of effect；At least one light feeding unit, its fluid sample being oriented into the suction pipette head is provided Light；And at least one light collector unit, it is oriented to from the liquid sample collection light in the suction pipette head.Component Unit can as a part for manufacturing process but it is removable or be integrated into suction pipette head.

In another embodiment, this disclosure provides a kind of by independent suction pipette head insertion light measurement system In method, comprise the following steps：There is provided by being configured to receive the suction pipette head that the base unit of suction pipette head is constituted Device, the base unit be configured to provide the suction pipette head be installed to the light feeding unit of the base unit and Structure alignment between light collector unit, wherein the base unit has the in-profile for limiting receiving space, and wherein The in-profile of the base unit is configured to physically interact with the exterior contour of assembly unit, the assembly unit It is configured to fix suction pipette head and inserts suction pipette head in the suction pipette head device.

Brief description of the drawings

When considered in conjunction with the accompanying drawings, by reference to described in detail below, those of ordinary skill in the art can be preferably geographical The solution present invention, wherein：

Fig. 1 shows the cross-sectional view of the exemplary embodiment of suction pipette head measuring system.

Fig. 2 shows the front view of the exemplary embodiment of measuring system.

Fig. 3 shows the front view of the exemplary embodiment of measuring system.

Fig. 4 shows the front view of the exemplary embodiment of measuring system.

Fig. 5 shows box (cartridge) isometric view.

Fig. 6 shows the isometric view of pedestal.

Fig. 7 shows the exploded isometric view of measuring system.

Embodiment

Referring now to Figure 1, there is provided the exemplary embodiment for the measuring system 100 being made up of base unit 110, in pedestal Suction pipette head 130 is may be inserted into unit 110, it is therefore an objective to measure light transmission in a repeatable fashion.Base unit 110 is provided At least one light feeding unit 140 of light is provided by using the fluid sample being oriented into suction pipette head and is positioned Into the pipettor measured from least one light collector unit 150 of the liquid sample collection light in suction pipette head 130 Suction head apparatus.

Exemplary measurement system 100 can include such as base unit 110 and assembly unit 120.Assembly unit 120 by with It is set to fixed suction pipette head 130.In this context it is fixed mean assembly unit 120 can be suction pipette head 130 can Remove or integrated part.

If removable, assembly unit 120 can especially be configured to fix suction pipette head 130.Pipettor is inhaled First 130 can be fixed to assembly unit 120 by any conventional meanses --- if removable.Suction pipette head 130 can for example by being clasped, frictional fit or with impermanent mode by two be partially bonded together it is any other Similar mechanical means and be fixed on assembly unit 120 --- if removable.Alternatively, assembly unit 120 can To be one fixed permanently to suction pipette head 130 during manufacturing process.

The exterior contour 125 of assembly unit 120 may be configured to the inner wheel with the receiving space in base unit 110 Exterior feature 115 physically interacts.Between the exterior contour 125 of assembly unit 120 and the in-profile 115 of base unit 110 This Physical interaction can be in the light collector unit 150 and light being fixedly or movably arranged in base unit 110 The controlled structures alignment of pipette tip 130 is provided between feeding unit 140.

It is shown by between the exterior contour 125 of assembly unit 120 and the in-profile 115 of base unit and described The advantage of Physical interaction be：It provides substantially self aligned knot for the repeatable positioning of suction pipette head 130 Structure, which substantially eliminates the demand that may be needed in all conventional microlitre of volume droplet methods as discussed above, to use Family by suction pipette head 130 guide to thereon can be with specific base (pedestal) of deposited samples volume droplet when it is especially smart Really.

It is according to another advantage of the exemplary physical embodiment of present disclosure：They further obviate for from Suction pipette head sprays the demand of liquid sample material, so as to further obviate appearance of the cleaning for example in base unit 110 Receive the demand for services of the surface in space or any associated construction package of exemplary measurement system 100, as depicted As.In other words, fluid sample does not need any surface of contact structures, but can substantially protect in all cases Hold in suction pipette head 130.

Generally, the liquid for pipettor that disclosed suction pipette head 130 is suitable on pipettor, which is collected, to be inhaled Head.In embodiment, suction pipette head 130 can be attached to pipettor and/or be removed from pipettor.Standard side can be used Method realizes the attachment of suction pipette head 130, and the standard method is for example including snapping fit onto pipettor or press-fit To pipettor.Broadly, suction pipette head 130 can pass through physical pressure, magnetic force, gravity, suction or any similar Method come be maintained at suction pipette head 130 surface or pipettor main body sheet with appropriate location.When removable from pipettor Except/it is detachable when, suction pipette head 130 can be disposable assembly.Otherwise, when removable/detachable from pipettor, liquid relief Device suction nozzle 130 can be cleanable and reusable component.Suction pipette head 130 can be formed by any geometry, So as to substantially prevent fluid sample from pipettor spray when loss.

Suction pipette head 130 can be formed by optically transparent material.Suction pipette head 130 can be to it one or more Appropriate transmitance region or feature are applied in region, so that light is passed through with minimum interruption.

Fig. 2 shows the first exemplary embodiment 200 of the exemplary suction pipette head 230 according to present disclosure.Such as Shown in Fig. 2, exemplary suction pipette head 230 can be formed or otherwise be configured to have at least one flat site (parallel plane) 232,234.The optical clear region of exemplary suction pipette head 230 can be with specifying but different horizontal The parallel plane 232,234 of cross-sectional length.Parallel plane 232,234 can be by plastics, glass, ceramic or any similar flat Transparent material is formed.There may be one or more regions, these parallel planes 232,234 are so that multiple distances are separated and are formed In suction pipette head 230.Configuration based on measuring system, parallel plane 232,234 can be received with multipair smooth feeding unit/light Collect unit 240/250,245/255 to be aligned.

Referring now to Figure 2, it should be noted that multipair smooth feeding unit/light collector unit 240/250,245/255 need not edge Fluid sample identical, to penetrate axis aligned with each other.On the contrary, they can be aligned to wear in a lateral direction with various angles Saturating suction pipette head 230 and fluid sample.

Parallel plane 232,234 can transmit the wavelength for the light for being used for fluid sample under observation, measurement and analysis.It is preferred that Embodiment can include one or more parallel planes 232,234, and it transmits the wavelength of whole scope, i.e., quartzy.At this In the embodiment of sample, the easily fabricated and compatible of material can be the problem of easily solution.For example, some plastics can be saturating Penetrate high wave-length coverage.Polypropylene and some makrolon for example can be used to form parallel plane 232,234.Parallel plane 232, 234 can form according to conventional manufacturing process, including such as being blow molded, injection moulding, deposition modeling.

Parallel plane 232,234 can be formed and/or place especially to limit one or many by fluid sample Individual known cross-sectional path length.

Referring now to Figure 3, there is provided the second exemplary implementation of the exemplary suction pipette head 330 according to present disclosure Example 300.As shown in figure 3, exemplary suction pipette head 330 can be formed or otherwise be configured to have at least one Individual flexible region 332.The optical clear region of exemplary suction pipette head 330 can be by being received with a pair of smooth feeding unit/light Collect the interaction of unit 340/350 but moveable, so that specifies the fluid sample under observation, measurement and analysis can Variable cross-section length.It should be noted that the position of one or two in light feeding unit 340 and light collector unit 350 can phase Position for suction pipette head 330 is moveable, to be squeezed between light feeding unit 340 and light collector unit 350 At least one flexible region 332, so as to change path length.

At least one flexible region 332 in suction pipette head 330 can include one or more flexible regions, and it passes through Using appropriate light transmission but it is optically transparent and can be formed by any appropriate flexible material.Flexible region (or pulling) can suitably be extruded between the optics for including light feeding unit 340 and light collector unit 350 Different distance, or realized suitably by other means.

Referring now to Figure 4, showing the 3rd exemplary implementation according to the exemplary suction pipette head 430 of present disclosure Example 400.As shown in figure 4, exemplary suction pipette head 430 can be formed in light feeding unit 440 and light collector unit 450 Between at least one observation area 432 include one or more optical modules 436,438 (not necessarily to scale), optical module 436,438 can be used for specify when light observation, measurement and analysis under pass through fluid sample when focus/path length and focus/ Path characteristics.

In these embodiments, one or two in light feeding unit and light collector unit can include some optics devices Part adjusting part, including but not limited between light feeding unit and suction pipette head and/or suction pipette head and light collector unit Between one or more lens, speculum, window, and/or filter.

It would be recognized by those skilled in the art that the physical length and focal length through the change of the light transmission path of fluid sample can To improve the precision of illumination/absorptiometry for fluid sample.

Embodiment 1 uses measurement of the method for self-locating by the quartzy luminous intensity with known paths length

Referring now to Figure 5, the box 500 being made up of workpiece 510 and two quartz disks 520 are shown, two quartz disks 520 It is assembled into watertightness (water tight), is open to sink by the hole 530 in the top of workpiece 510 with one Hydrops body.Workpiece 510 has the antelabium 540 of known thickness, and a quartz disk 520 adheres to it per side.This configuration exists Known path length is generated between two inner surfaces of quartz disk.

Referring now to Figure 6, showing by mach substrate 610, two mach bearing blocks 620, linear bearings 630th, the pedestal 600 that two accurate axles 640 and two screw thread ferrules 650 are constituted.Bearing block 620 has lip on side Edge 660, and have through the screwed hole at center to be attached screw thread ferrule 650.

Referring now to Figure 7, showing by the way that the optical fiber cable 710 with quartzy core is connected at one end into light source 720, simultaneously And the measuring system 700 for being connected to one in screw thread ferrule 650 in the other end and producing.With more than with quartzy core The second optical fiber cable 730 of optical fiber cable 710 be connected at one end to remaining screw thread ferrule 650, and in the other end It is connected to spectrometer 740.Graphic rendition (the graphical of the signal for being suitable for spectrometer 740 of spectrometer 740 Interpretation software) and be fed in personal computer 750, to measure luminous intensity.

Pass through the minimum support for the loose fit slit (loose fitting slot) being machined in substrate 610, box 500 It is placed on the approximate center between two bearing blocks 620.Bearing block 620 is moved along linear bearing 630, to allow antelabium 660 contact with quartz disk 520.This interaction makes the optical fiber cable 710 and 730 with quartzy core be hung down with quartz disk 520 Straight alignment.The measurement of luminous intensity is obtained from the configuration.

Measured to be compared with the air between the quartz disk 520 and water in box 500.Between each measurement, Box 500 is removed and changed from pedestal 600, to produce the unduplicated original position of box 500 for measurement every time.Can be with The result of these measurements is found out in table 1.

Table 1

Result in table 1 corresponds to the corresponding linear tropic each with the R square values more than 0.9.It is familiar with this value People will appreciate that in the presence of a clear and definite linear trend.It will be produced with the intensity level of time integral using personal computer 750 Accurate ionization meter, this then produces accurate absorbance/fluorescence measurement.

Example 1, which is similar to, inserts suction pipette head in one mechanism, and necessary vertical to produce dependent on mechanism Degree.

For example to the embodiment discussed above for disclosed suction pipette head and its feature with specific reference to should It is interpreted disclosed suction pipette head being only limitted to those embodiments.Describe and the embodiment that describes is included to pair The disclosed product for being used to realize the system for being used for fluid sample observation, measurement and analysis, method, technology, process and scheme Non-limitative illustration, therefore should be construed as merely exemplary, and do not limit disclosed side in any way Case.

The feature and advantage of the disclosed embodiments are set forth in this disclosure, and can be at least in part from this It is clear that or can be learned by putting into practice the disclosed embodiments in detailed description.The feature of the disclosed embodiments It can realize and obtain by the instrument that is particularly described and the combination of feature with advantage.

The various embodiments of disclosed system and method are discussed in this disclosure.Although discussing specific reality It is existing, but it is to be understood that this purpose being merely to illustrate that.Those skilled in the relevant art are not it will be recognized that departing from In the case of the spirit and scope of disclosed embodiment, other assemblies and configuration can be used.

Claims (12)

1. a kind of suction pipette head device, including：It is configured to receive the base unit of suction pipette head, the base unit quilt It is configured to be installed to knot that the suction pipette head is provided between the light feeding unit of the base unit and light collector unit Structure is aligned, wherein the base unit has the in-profile for limiting receiving space, wherein the inside of the base unit The exterior contour that profile is configured to and be configured to fix the assembly unit of suction pipette head physically interacts.

2. suction pipette head device according to claim 1, in addition to：Pipettor suitable for being attached to pipettor is inhaled Head, wherein the suction pipette head includes at least one being positioned between the smooth feeding unit and the smooth collector unit At least one optical module in observation area.

3. suction pipette head device according to claim 1, in addition to：Pipettor suitable for being attached to pipettor is inhaled Head, wherein the suction pipette head is configured to have at least one optically transparent flat site.

4. suction pipette head device according to claim 1, in addition to：Pipettor suitable for being attached to pipettor is inhaled Head, wherein the suction pipette head be configured to have at least one flexible region, at least one described flexible region with least One light feeding unit contacts to change the path length by the fluid sample in the suction pipette head.

5. suction pipette head device according to claim 1, in addition to：Pipettor suitable for being attached to pipettor is inhaled Head, wherein the suction pipette head be configured to have at least one flexible region, at least one described flexible region with least One light collector unit contact, to change the path length by the fluid sample in the suction pipette head.

6. a kind of suction pipette head measuring system, including：Base unit with the in-profile for limiting receiving space；It is configured Into the assembly unit of fixed suction pipette head, and the component has the in-profile thing being configured to the base unit The exterior contour of reason ground interaction；At least one light feeding unit, it is oriented to the liquid into the suction pipette head Sample provides light；And at least one light collector unit, it is oriented to collect the liquid-like in the suction pipette head The light of product, in addition to suitable for being attached to the suction pipette head that the optically transparent liquid of pipettor is collected, wherein the liquid relief Device suction nozzle is configured to have at least one flexible region.

7. suction pipette head measuring system according to claim 6, wherein, the smooth feeding unit and the light are collected single Member is installed to the base unit, and the base unit is configured to provide the structure alignment of the suction pipette head.

8. suction pipette head measuring system according to claim 6, wherein, the suction pipette head is configured to have extremely A few flat site.

9. suction pipette head measuring system according to claim 6, wherein, the suction pipette head is configured to have extremely Few two parallel flat sites.

10. suction pipette head measuring system according to claim 6, wherein, at least one described flexible region with least One light feeding unit contacts to change the path length by the fluid sample.

11. suction pipette head measuring system according to claim 6, wherein, at least one described flexible region with least One light collector unit contacts to change the path length by the fluid sample.

12. suction pipette head measuring system according to claim 6, wherein, the suction pipette head includes being positioned in At least one optical module at least one observation area between the smooth feeding unit and the smooth collector unit.