CN105598138A - Eggshell recycling method and application thereof - Google Patents
Eggshell recycling method and application thereof Download PDFInfo
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- CN105598138A CN105598138A CN201610117033.3A CN201610117033A CN105598138A CN 105598138 A CN105598138 A CN 105598138A CN 201610117033 A CN201610117033 A CN 201610117033A CN 105598138 A CN105598138 A CN 105598138A
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- eggshell
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- eggshells
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- 210000003278 egg shell Anatomy 0.000 title claims abstract description 64
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 61
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 39
- 238000004064 recycling Methods 0.000 title abstract 5
- 229940012466 egg shell membrane Drugs 0.000 claims abstract description 30
- 102000004190 Enzymes Human genes 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 19
- 230000007062 hydrolysis Effects 0.000 claims abstract description 18
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims abstract description 11
- 239000007791 liquid phase Substances 0.000 claims abstract description 8
- 244000005700 microbiome Species 0.000 claims abstract description 4
- 239000007790 solid phase Substances 0.000 claims abstract description 4
- 238000011084 recovery Methods 0.000 claims description 19
- 229940088598 enzyme Drugs 0.000 claims description 17
- 239000004365 Protease Substances 0.000 claims description 14
- 238000000926 separation method Methods 0.000 claims description 11
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- 235000012054 meals Nutrition 0.000 claims description 9
- 229920001169 thermoplastic Polymers 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- 239000004416 thermosoftening plastic Substances 0.000 claims description 7
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 235000019419 proteases Nutrition 0.000 claims description 5
- 229920002385 Sodium hyaluronate Polymers 0.000 claims description 4
- 239000000945 filler Substances 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- -1 polyethylene Polymers 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 4
- 229940010747 sodium hyaluronate Drugs 0.000 claims description 4
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- ZHQQRIUYLMXDPP-SSDOTTSWSA-N Actinidine Natural products C1=NC=C(C)C2=C1[C@H](C)CC2 ZHQQRIUYLMXDPP-SSDOTTSWSA-N 0.000 claims description 3
- 108010004032 Bromelains Proteins 0.000 claims description 3
- 108090000270 Ficain Proteins 0.000 claims description 3
- 108090000526 Papain Proteins 0.000 claims description 3
- 108090000284 Pepsin A Proteins 0.000 claims description 3
- 102000057297 Pepsin A Human genes 0.000 claims description 3
- 235000006886 Zingiber officinale Nutrition 0.000 claims description 3
- 108090000350 actinidain Proteins 0.000 claims description 3
- 235000019835 bromelain Nutrition 0.000 claims description 3
- 235000019836 ficin Nutrition 0.000 claims description 3
- POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 235000008397 ginger Nutrition 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 235000019834 papain Nutrition 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 229940111202 pepsin Drugs 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims description 2
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- 239000004626 polylactic acid Substances 0.000 claims description 2
- 229920000642 polymer Polymers 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 244000273928 Zingiber officinale Species 0.000 claims 1
- 239000004800 polyvinyl chloride Substances 0.000 claims 1
- 229920000915 polyvinyl chloride Polymers 0.000 claims 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 claims 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 claims 1
- 238000005201 scrubbing Methods 0.000 claims 1
- 239000002699 waste material Substances 0.000 abstract description 11
- 239000005416 organic matter Substances 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 5
- 239000012528 membrane Substances 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract 2
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 1
- 238000005191 phase separation Methods 0.000 abstract 1
- 102000008186 Collagen Human genes 0.000 description 4
- 108010035532 Collagen Proteins 0.000 description 4
- 229920001436 collagen Polymers 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 108010014258 Elastin Proteins 0.000 description 3
- 102000016942 Elastin Human genes 0.000 description 3
- 101710097834 Thiol protease Proteins 0.000 description 3
- 235000013601 eggs Nutrition 0.000 description 3
- 229920002549 elastin Polymers 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 229920002683 Glycosaminoglycan Polymers 0.000 description 2
- 241000234314 Zingiber Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000000969 egg white Anatomy 0.000 description 2
- 235000014103 egg white Nutrition 0.000 description 2
- 229920002674 hyaluronan Polymers 0.000 description 2
- 229960003160 hyaluronic acid Drugs 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 230000037303 wrinkles Effects 0.000 description 2
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229920001287 Chondroitin sulfate Polymers 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 208000010412 Glaucoma Diseases 0.000 description 1
- 229920000288 Keratan sulfate Polymers 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 230000002308 calcification Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940059329 chondroitin sulfate Drugs 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000007822 coupling agent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003063 flame retardant Substances 0.000 description 1
- 239000004088 foaming agent Substances 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000012760 heat stabilizer Substances 0.000 description 1
- 238000010102 injection blow moulding Methods 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- KXCLCNHUUKTANI-RBIYJLQWSA-N keratan Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@H](COS(O)(=O)=O)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@H](O[C@@H](O[C@H]3[C@H]([C@@H](COS(O)(=O)=O)O[C@@H](O)[C@@H]3O)O)[C@H](NC(C)=O)[C@H]2O)COS(O)(=O)=O)O[C@H](COS(O)(=O)=O)[C@@H]1O KXCLCNHUUKTANI-RBIYJLQWSA-N 0.000 description 1
- 239000004611 light stabiliser Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000012492 regenerant Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000037204 skin physiology Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01F—COMPOUNDS OF THE METALS BERYLLIUM, MAGNESIUM, ALUMINIUM, CALCIUM, STRONTIUM, BARIUM, RADIUM, THORIUM, OR OF THE RARE-EARTH METALS
- C01F11/00—Compounds of calcium, strontium, or barium
- C01F11/18—Carbonates
- C01F11/185—After-treatment, e.g. grinding, purification, conversion of crystal morphology
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/80—Destroying solid waste or transforming solid waste into something useful or harmless involving an extraction step
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B5/00—Operations not covered by a single other subclass or by a single other group in this subclass
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01F—COMPOUNDS OF THE METALS BERYLLIUM, MAGNESIUM, ALUMINIUM, CALCIUM, STRONTIUM, BARIUM, RADIUM, THORIUM, OR OF THE RARE-EARTH METALS
- C01F11/00—Compounds of calcium, strontium, or barium
- C01F11/18—Carbonates
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0072—Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/24—Acids; Salts thereof
- C08K3/26—Carbonates; Bicarbonates
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2006/00—Physical properties of inorganic compounds
- C01P2006/80—Compositional purity
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/24—Acids; Salts thereof
- C08K3/26—Carbonates; Bicarbonates
- C08K2003/265—Calcium, strontium or barium carbonate
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Environmental & Geological Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Inorganic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Materials Engineering (AREA)
- Geology (AREA)
- Zoology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Processing Of Solid Wastes (AREA)
- Cosmetics (AREA)
Abstract
The invention provides an eggshell recycling method and application thereof, and belongs to the field of waste material recycling. The method comprises the following steps that 1, eggshells with membranes are selected and recycled, and then the selected eggshells are washed and sterilized so as to remove dirt and microorganisms; 2, the eggshells are smashed so as to increase the specific surface area; 3, enzyme hydrolysis is conducted, the contact area of the smashed eggshells is increased, and the hydrolysis process can be accelerated, so that the enzyme hydrolysis time is shortened, and organic matter similar to a liquid phase and unhydrolyzed inorganic matter are obtained after hydrolysis; 4, solid-liquid phase separation is conducted on substances obtained through hydrolysis; 5, the liquid phase is taken and dried, and eggshell membrane powder is obtained; 6, the solid phase is taken and dried, and eggshell powder is obtained. The eggshell recycling method and the application thereof have the advantages that waste materials can be recycled and utilized to the maximum extent; the eggshells and eggshell membranes do not need to be separated for recycling, hydrolysis is achieved through an enzymatic hydrolysis method, the organic matter cannot be destroyed, and the organic part and the inorganic part are dried separately, so that the substances which are different in application are obtained.
Description
Technical field
The invention belongs to waste recovery field, specifically, relate to a kind of recovery of eggshell and the purposes of regenerant.
Background technology
Egg is made up of yolk, egg white, two-layer egg shell membrane and eggshell, and egg is as the regular guest on every dining table, and consumption is non-Often huge, after the finished Huang of culinary art and egg white, remaining,, generally as refuse processing, can not reclaim, but in fact, eggshellBe worth and underestimated for obsolete material, in the report egg shell membrane such as Osuoji, have acid glycosamine glycan, and glycosaminoglycan is (, saturatingPhaneroplasm acid) likely keep for moisture content and opposing bacteria attack, after this, more research is reported, egg shell membrane contains manyValuable compounds therefrom, if crosslinked with collagen albumen (I, V and X), glycosaminoglycan (are hyaluronic acid, chondroitin sulfate, acetyl sulfateHeparin and keratan sulfate), albumen protein (for example ovotransferrins, lysozyme) and eggshell stromatin (for example ovum calcium eggIn vain-36(ovocalyxin-36)).
According to the report of Hong Kong import trade statistical data in 2013, approximately 2,243, the band shell of 000,000 unitBirds, beasts and eggs import is to Hong Kong, and this expression exists the huge refuse amount (every egg shell approximately 6 grams) of eggshell, because in Hong Kong and ChinaThere are a large amount of cakes and bread manufacturer, so collect stay-in-grade eggshell waste material than other food waste also relatively easy obtainingMany. Therefore the reconstituted circularly product that, this eggshell waste material should be fully utilized and change into variety classes and can be widely used.
In order better to utilize discarded eggshell, many researchers and many companies develop different eggshells and eggshellMembrane separating method, is intended to be obtained and better reclaimed product by separation. As MacNil has developed a kind of for removing egg shell particleThe method and apparatus of membrane portions, film can be separated with eggshell based on diffeential floatation. DeJong and Vlad are for to be equipped with fluidKnockout drum in the eggshell processed in unsegregated eggshell co-current flow body apply cavitation effect and egg shell membrane separated to Shen with eggshellPlease patent. Snyder is by regulating the water content of eggshell, then applies air-flow and egg shell membrane is separated with eggshell. But, pointFrom after reclaim again, even if dry, crushing, grinding and overturning (with film) eggshell has valuable organic content in egg shell membraneThing, as protein, collagen, elastin laminin and hyaluronic egg shell membrane also may still be attached on eggshell securely,Can not reclaim completely, and eggshell is still to abandon as waste material.
Summary of the invention
The object of the present invention is to provide a kind of while can fully reclaim the method for eggshell and egg shell membrane, and, thisThe bright method providing, without eggshell is separated with egg shell membrane, is extracted useful constituent from eggshell waste material (eggshell and egg shell membrane), as carbonAcid calcium, egg shell membrane powder (containing protein, collagen, elastin laminin, hyaluronic acid), reclaim the abundantest calcium carbonate that containsUseful calcification egg-shell meal, and can increase and extract in then eggshell and egg shell membrane be dried to egg shell membrane powder valuable organicTolerant, as protein, collagen, elastin laminin, hyaluronic amount.
Method provided by the invention is as follows:
A kind of eggshell waste material recovery method, described recovery method comprises the steps:
(1) choose and reclaim band film eggshell, washing, sterilizing, to remove dirt and microorganism;
(2) pulverize eggshell to increase specific area;
(3) enzyme hydrolysis, the eggshell contact area after pulverizing increases, and can accelerate hydrolytic process, to shorten the time of enzyme hydrolysis; WaterThe inorganic matter that obtains the organic matter of similar liquid phase after solution and be not hydrolyzed;
(4) material after hydrolysis is carried out to Solid-Liquid Separation;
(5) get liquid phase, after being dried, obtain egg shell membrane powder;
(6) get solid phase, after being dried, obtain egg-shell meal.
Further, described recovery method also comprises the steps: to dissolve described egg shell membrane powder, separation, purifying, dryAfter dry, obtain Sodium Hyaluronate, its purity is more than 90%.
Sodium Hyaluronate is the biochemical drug with higher clinical value, is widely used in all kinds of ophthalmologic operations, as crystalImplantation, corneal transplantation and resisting glaucoma operation etc., also can be used for treatment of arthritis and accelerating wound healing. Use it for cosmeticsIn, can play unique protection skin effect, can keep skin moisturizing smooth, fine and smooth tender, high resilience, has wrinkle resistant, anti-The effect of wrinkle, beauty and health care and recovery skin physiology function.
Further, in step (2), after eggshell pulverizing, particle diameter is 0.1 to 99 micron, to shorten the time of enzyme hydrolysis.
Further, the enzyme adopting in step (3) is the protease with at least one sulfydryl, as Actinidin,More than one enzymes of bromelain, ficin, papain, pepsin or Ginger Protease, single sulfydrylThe combination of protease or thiol protease is hydrolyzed to increase for enzyme-specific and extracts from eggshell waste material (eggshell and egg shell membrane twoPerson) the dissolubility of organic content, and can not digest in large quantities valuable organic content.
Further, in step (4), separate the method adopting and have suction strainer separation, press filtration separation or centrifugation.
Further, in step (5) and (6), dry method has spraying to be dried or freeze drying.
The purposes that adopts egg-shell meal prepared by above-mentioned method, is characterized in that, for replacing existing calcium carbonate polymerizationThing filler is prepared thermoplastic, the egg-shell meal that this method makes, particle diameter be 0.1 to 99 micron and calcium carbonate content be 90% to99%, contain seldom or do not contain organic content, can be for replacing existing calcium carbonate polymer filler and using single or twoScrew extruder is sneaked in thermoplastic product.
Further, thermoplastic adopts polyethylene, PLA, polypropylene, poly-(ethene-ester vinyl acetate) or polychlorostyreneThe material that ethene is prepared from. The constituent of described thermoplastic is made up of 20% to 60% egg-shell meal, also can containOther additive component, as antimicrobial, antistatic additive, coupling agent, dyestuff and pigment, filler, fire retardant, foaming agent,Heat stabilizer, nucleator, smell releasing agent, ultraviolet ray or light stabilizer, thermoplastic polymer composite material can be by extruding,Injection moulding, blow molding and fusion sediment moulding are further processed to make thermoplastic product.
Beneficial effect of the present invention is, 1. can reclaim and utilize by maximum refuse; 2. without by eggshell and eggShell membrane reclaims after separating again, still can't destroy organic matter wherein by the method hydrolysis of enzyme hydrolysis, then dry respectivelyOrganic moiety and inorganic part after, obtain the material that use is different.
Brief description of the drawings
Fig. 1 is process chart of the present invention.
Detailed description of the invention
Embodiment 1
Below in conjunction with accompanying drawing, to this case further instruction.
As accompanying drawing process chart, a kind of eggshell waste material recovery method, described recovery method comprises the steps:
(1) choose and reclaim band film eggshell, washing, sterilizing, to remove dirt and microorganism;
(2) pulverize eggshell to increase specific area, it is 0.1 to 99 micron that eggshell is pulverized rear particle diameter, to shorten in next stepThe time of enzyme hydrolysis;
(3) enzyme hydrolysis, the eggshell contact area after pulverizing increases, and can accelerate hydrolytic process, to shorten the time of enzyme hydrolysis, waterThe inorganic matter that obtains the organic matter of similar liquid phase after solution and be not hydrolyzed, the enzyme of employing is the albumen with at least one sulfydrylEnzyme, as one of Actinidin, bromelain, ficin, papain, pepsin or Ginger ProteasePlant above enzyme, the combination of single thiol protease or thiol protease is hydrolyzed to increase for enzyme-specific and extracts from eggshell waste materialThe dissolubility of organic content of (eggshell and egg shell membrane), and can not digest in large quantities valuable organic content;
(4) material after hydrolysis is carried out to Solid-Liquid Separation, separating the method adopting has suction strainer separation, press filtration to separate or centrifugal pointFrom;
(5) get liquid phase, after spray dry or freeze drying, obtain egg shell membrane powder;
(6) get solid phase, after spray dry or freeze drying, obtain egg-shell meal.
Embodiment 2
After six steps in embodiment 1, obtain egg shell membrane powder, the egg shell membrane powder of gained is dissolved, further separation, pureAfter changing, being dried, obtain Sodium Hyaluronate, purity is more than 90%.
Claims (9)
1. an eggshell recovery method, is characterized in that, described recovery method comprises the steps:
(1) choose recovery eggshell, washing, sterilizing, remove dirt and microorganism;
(2) eggshell after sterilizing scrubbing is pulverized;
(3) by the eggshell enzyme hydrolysis after pulverizing;
(4) by the material Solid-Liquid Separation after hydrolysis;
(5) get liquid phase, after being dried, obtain egg shell membrane powder;
(6) get solid phase, after being dried, obtain egg-shell meal.
2. recovery method as claimed in claim 1, is characterized in that, described in described recovery method also comprises the steps:The further separation of egg shell membrane powder, purifying, after dry Sodium Hyaluronate.
3. recovery method as claimed in claim 1, is characterized in that, in step (2) eggshell pulverize after particle diameter be 0.1 to 99 micro-Rice.
4. recovery method as claimed in claim 1, is characterized in that, the enzyme adopting in step (3) is for having at least one mercaptoThe protease of base.
5. recovery method as claimed in claim 4, is characterized in that, described enzyme be Actinidin, bromelain,More than one enzymes of ficin, papain, pepsin or Ginger Protease.
6. recovery method as claimed in claim 1, is characterized in that, separate in step (4) method adopting have suction strainer separate,Press filtration separation or centrifugation.
7. recovery method as claimed in claim 1, is characterized in that, in step (5) and (6), dry method has spraying dryOr freeze drying.
8. the purposes of the egg-shell meal of preparing according to the method described in the arbitrary claim of claim 1-7, is characterized in that, forReplace calcium carbonate polymer filler to prepare thermoplastic.
9. the purposes of egg-shell meal as claimed in claim 8, is characterized in that, thermoplastic adopts polyethylene, PLA, poly-The material that propylene, poly-(ethene-ester vinyl acetate) or polyvinyl chloride are prepared from.
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| CN201610117033.3A CN105598138A (en) | 2016-03-02 | 2016-03-02 | Eggshell recycling method and application thereof |
| CN201710106786.9A CN107043798B (en) | 2016-03-02 | 2017-02-27 | Eggshell recovery method and application of recovered material |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107043798A (en) * | 2016-03-02 | 2017-08-15 | 世界家庭用具制品厂有限公司 | A kind of purposes of eggshell recovery method and regenerant |
| WO2017148343A1 (en) * | 2016-03-02 | 2017-09-08 | 世界家庭用具制品厂有限公司 | Eggshell recycling method and uses of recycled material |
| ES2633062A1 (en) * | 2017-07-12 | 2017-09-18 | Eggnovo S.L. | PROCEDURE AND COMPOSITION OF HYDROLYZING EGG SHELL MEMBRANE (Machine-translation by Google Translate, not legally binding) |
| WO2022229326A3 (en) * | 2021-04-29 | 2023-03-23 | Avgo Biotech Ltd | Apparatus and method for producing purified calcium carbonate |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108323764B (en) * | 2018-01-19 | 2021-06-04 | 湖北神地生物科技有限公司 | Method for extracting eggshell membrane polypeptide and preparing bone joint health product |
| JP6560837B1 (en) * | 2019-01-15 | 2019-08-14 | 株式会社 アルマード | Egg shell membrane lot manufacturing method and egg related product group manufacturing method |
| CN109970883A (en) * | 2019-03-13 | 2019-07-05 | 世界家庭用具制品厂有限公司 | A method of by recycling Sodium Hyaluronate and its isolation and purification in eggshell |
| CN112195214B (en) * | 2020-09-01 | 2021-12-03 | 华南理工大学 | Fish skin collagen polypeptide and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2006158354A (en) * | 2004-12-10 | 2006-06-22 | Robotsuto Meeshiyon Kk | Method for extracting eggshell membrane hydrolysis protein containing collagen from eggshell membrane using ultrasonic wave |
| US7584909B2 (en) * | 2005-01-18 | 2009-09-08 | Biova, L.L.C. | Eggshell membrane separation method |
| CN105671018A (en) * | 2010-08-31 | 2016-06-15 | 天野酶制品株式会社 | Eggshell membrane solubilization method using enzymes |
| CN102399281A (en) * | 2010-09-07 | 2012-04-04 | 丁庆 | Manufacturing method of water-soluble eggshell membrane polypeptide |
| CN102898540A (en) * | 2011-07-29 | 2013-01-30 | 肖汉 | Extraction technology of hyaluronic acid |
| CN103059606B (en) * | 2012-12-24 | 2014-10-08 | 暨南大学 | Rigid composite particle materials containing natural biological calcification materials and preparation method and application thereof |
| CN105598138A (en) * | 2016-03-02 | 2016-05-25 | 世界家庭用具制品厂有限公司 | Eggshell recycling method and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107043798A (en) * | 2016-03-02 | 2017-08-15 | 世界家庭用具制品厂有限公司 | A kind of purposes of eggshell recovery method and regenerant |
| WO2017148343A1 (en) * | 2016-03-02 | 2017-09-08 | 世界家庭用具制品厂有限公司 | Eggshell recycling method and uses of recycled material |
| CN107043798B (en) * | 2016-03-02 | 2021-08-31 | 纳米及先进材料研发院有限公司 | Eggshell recovery method and application of recovered material |
| ES2633062A1 (en) * | 2017-07-12 | 2017-09-18 | Eggnovo S.L. | PROCEDURE AND COMPOSITION OF HYDROLYZING EGG SHELL MEMBRANE (Machine-translation by Google Translate, not legally binding) |
| WO2019012170A1 (en) * | 2017-07-12 | 2019-01-17 | Eggnovo S.L. | Method and composition for hydrolyzing eggshell membrane |
| WO2022229326A3 (en) * | 2021-04-29 | 2023-03-23 | Avgo Biotech Ltd | Apparatus and method for producing purified calcium carbonate |
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| CN107043798B (en) | 2021-08-31 |
| CN107043798A (en) | 2017-08-15 |
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Application publication date: 20160525 |