CN109609578A - A method of the abstraction function albumen from inkfish - Google Patents
A method of the abstraction function albumen from inkfish Download PDFInfo
- Publication number
- CN109609578A CN109609578A CN201910037226.1A CN201910037226A CN109609578A CN 109609578 A CN109609578 A CN 109609578A CN 201910037226 A CN201910037226 A CN 201910037226A CN 109609578 A CN109609578 A CN 109609578A
- Authority
- CN
- China
- Prior art keywords
- inkfish
- abstraction function
- function albumen
- albumen
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to technical field of bioengineering, and in particular to a method of the abstraction function albumen from inkfish, including pre-treatment, soda acid processing, hot water extraction, enzymatic hydrolysis, reduced pressure and drying；Functional protein can be efficiently extracted out by this method from inkfish, can comprehensively utilize inkfish meat and inkfish capsule, improve inkfish resource utilization, effective solution inkfish ingredient development difficulty big problem.
Description
Technical field
The invention belongs to technical field of bioengineering, and in particular to a method of the abstraction function albumen from inkfish.
Background technique
Functional protein is the native biopolymer of zooblast synthesis, if collagen is as the weight in extracellular matrix
Want ingredient be almost present in it is organized in, account for about the 30% of animal total protein, there is high resistance to extend ability, make for collagen
The connective tissues such as skin are maintained certain structure, realize and support and protect cell, tissue and body function, to cell and group
The structure knitted and performance etc. are very crucial.Collagen peptide be using collagen as raw material using acid/base processing, enzymic degradation or
In conjunction with the mixtures of polypeptides that multiple form obtains, main component is the inhomogenous polypeptide of the molecular weight that is formed after collagen degradation.
Inkfish Functional Polypeptides can be used as a kind of functional food or health product raw material, can it is anti-oxidant, promote damaged tissues more
Close, inhibit arthritis, improve bone density etc..But the existing exploitation to inkfish resource and processing predominantly stay in first, the essence of inkfish
In processing, and raw material has seasonal, region, to the difficult point for being developed into the exploitation of marine functional peptide of inkfish effective component.
Summary of the invention
The method of the purpose of the present invention is to provide a kind of from inkfish abstraction function albumen, to solve above-mentioned background technique
The problem of middle proposition.
To achieve the above object, the invention provides the following technical scheme: a kind of method of the abstraction function albumen from inkfish,
Include the following steps:
S1, pre-treatment: inkfish is cleaned, defibrination crushes；
S2, soda acid processing: after taking 0.7 times of inkfish weight of food grade hydrochloric acid to handle half an hour, water is cleaned 2 times, then heavy with inkfish
It measures 1% food grade sodium hydroxide to be neutralized, be washed with water after half an hour, until pH is washed till neutrality；
S3, hot water extract: hot water is added, by the extraction of certain time, measures extracting solution PH；
S4, enzymatic hydrolysis: the alkali protease of extracting solution solid content weight 0.2%, 0.2% neutral proteinase is added；
S5, reduced pressure: enzymolysis liquid is concentrated to solid content using dual-effect concentrator；
S6, drying: make raw material using centrifugal spray drying, control the temperature and feed rate of feed inlet and outlet air, will be concentrated
Filtrate, which is spray-dried, is made albumen powder.
Preferably, the temperature of hot water described in S3 is 95 DEG C, and water is 2-3 times of inkfish weight, and water is inkfish weight
2-3 times, extraction time be 3 hours.
Preferably, hydrolysis temperature is 50 DEG C -55 DEG C in S4, and the time is 3 hours.
Preferably, solid content is 30%-40% after being concentrated in S5.
Preferably, inlet temperature described in S6 is 160-190 DEG C, and leaving air temp is 90 DEG C -100 DEG C, and charging rate is
180-220r/min。
Compared with prior art, the beneficial effects of the present invention are: this method can by functional protein from inkfish effectively
Extract, inkfish meat and inkfish capsule can be comprehensively utilized, improve inkfish resource utilization, effective solution inkfish at
The problem for dividing development difficulty big.
Detailed description of the invention
Fig. 1 is process flow diagram of the invention.
Specific embodiment
Below with reference to embodiment, the present invention will be further described.
The following examples are intended to illustrate the invention, but cannot be used to limit the scope of the invention.Item in embodiment
Part can be adjusted according to actual conditions are further, under concept thereof of the invention all to method simple modifications of the invention
Belong to the scope of protection of present invention.
Embodiment 1
Referring to Fig. 1, a kind of method of the abstraction function albumen from inkfish, includes the following steps:
S1, pre-treatment: inkfish is cleaned, defibrination crushes；
S2, soda acid processing: after taking 0.7 times of inkfish weight of food grade hydrochloric acid to handle half an hour, water is cleaned 2 times, then heavy with inkfish
It measures 1% food grade sodium hydroxide to be neutralized, be washed with water after half an hour, until pH is washed till neutrality；
S3, hot water extract: 95 DEG C of hot water are added, water is 2-3 times of inkfish weight, and by extraction in 3 hours, measurement was extracted
Liquid PH；
S4, enzymatic hydrolysis: being added the alkali protease of extracting solution solid content weight 0.2%, 0.2% neutral proteinase, and hydrolysis temperature is
50 DEG C -55 DEG C, the time is 3 hours；
S5, reduced pressure: being concentrated to solid content for enzymolysis liquid using dual-effect concentrator, and solid content is 30%-40% after concentration；
S6, drying: making raw material using centrifugal spray drying, and control inlet temperature is 160-190 DEG C, and leaving air temp is 90 DEG C-
100 DEG C, charging rate 180-220r/min, concentration filtrate is spray-dried, albumen powder is made.
Embodiment 2
Referring to Fig. 1, a kind of method of the abstraction function albumen from inkfish, includes the following steps:
S1, pre-treatment: inkfish is cleaned, defibrination crushes；
S2, soda acid processing: after taking 0.7 times of inkfish weight of food grade hydrochloric acid to handle half an hour, water is cleaned 2 times, then heavy with inkfish
It measures 1% food grade sodium hydroxide to be neutralized, be washed with water after half an hour, until pH is washed till neutrality；
S3, hot water extract: 95 DEG C of hot water are added, water is 2 times of inkfish weight, by extraction in 3 hours, measures extracting solution
PH；
S4, enzymatic hydrolysis: being added the alkali protease of extracting solution solid content weight 0.2%, 0.2% neutral proteinase, and hydrolysis temperature is
50 DEG C, the time is 3 hours；
S5, reduced pressure: being concentrated to solid content for enzymolysis liquid using dual-effect concentrator, and solid content is 30% after concentration；
S6, drying: making raw material using centrifugal spray drying, and control inlet temperature is 190 DEG C, and leaving air temp is 100 DEG C, charging
Speed is 220r/min, concentration filtrate is spray-dried, albumen powder is made.
Embodiment 3
Referring to Fig. 1, a kind of method of the abstraction function albumen from inkfish, includes the following steps:
S1, pre-treatment: inkfish is cleaned, defibrination crushes；
S2, soda acid processing: after taking 0.7 times of inkfish weight of food grade hydrochloric acid to handle half an hour, water is cleaned 2 times, then heavy with inkfish
It measures 1% food grade sodium hydroxide to be neutralized, be washed with water after half an hour, until pH is washed till neutrality；
S3, hot water extract: 95 DEG C of hot water are added, water is 3 times of inkfish weight, by extraction in 3 hours, measures extracting solution
PH；
S4, enzymatic hydrolysis: being added the alkali protease of extracting solution solid content weight 0.2%, 0.2% neutral proteinase, and hydrolysis temperature is
55 DEG C, the time is 3 hours；
S5, reduced pressure: being concentrated to solid content for enzymolysis liquid using dual-effect concentrator, and solid content is 40% after concentration；
S6, drying: making raw material using centrifugal spray drying, and control inlet temperature is 160 DEG C, and leaving air temp is 90 DEG C, charging
Speed is 180r/min, concentration filtrate is spray-dried, albumen powder is made.
Functional protein can be efficiently extracted out by this method from inkfish, can integrate inkfish meat and inkfish capsule
It utilizes, improves inkfish resource utilization, effective solution inkfish ingredient development difficulty big problem.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of method of the abstraction function albumen from inkfish, which comprises the steps of:
S1, pre-treatment: inkfish is cleaned, defibrination crushes；
S2, soda acid processing: after taking 0.7 times of inkfish weight of food grade hydrochloric acid to handle half an hour, water is cleaned 2 times, then heavy with inkfish
It measures 1% food grade sodium hydroxide to be neutralized, be washed with water after half an hour, until pH is washed till neutrality；
S3, hot water extract: hot water is added, by the extraction of certain time, measures extracting solution PH；
S4, enzymatic hydrolysis: the alkali protease of extracting solution solid content weight 0.2%, 0.2% neutral proteinase is added；
S5, reduced pressure: enzymolysis liquid is concentrated to solid content using dual-effect concentrator；
S6, drying: make raw material using centrifugal spray drying, control the temperature and feed rate of feed inlet and outlet air, will be concentrated
Filtrate, which is spray-dried, is made albumen powder.
2. a kind of method of abstraction function albumen from inkfish according to claim 1, which is characterized in that heat described in S3
The temperature of water is 95 DEG C, and extraction time is 3 hours.
3. a kind of method of abstraction function albumen from inkfish according to claim 1, which is characterized in that digest temperature in S4
Degree is 50 DEG C -55 DEG C, and the time is 3 hours.
4. a kind of method of abstraction function albumen from inkfish according to claim 1, which is characterized in that after being concentrated in S5
Solid content is 30%-40%.
5. a kind of method of abstraction function albumen from inkfish according to claim 1, which is characterized in that described in S6 into
Material mouth temperature is 160-190 DEG C, and leaving air temp is 90 DEG C -100 DEG C, charging rate 180-220r/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910037226.1A CN109609578A (en) | 2019-01-15 | 2019-01-15 | A method of the abstraction function albumen from inkfish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910037226.1A CN109609578A (en) | 2019-01-15 | 2019-01-15 | A method of the abstraction function albumen from inkfish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109609578A true CN109609578A (en) | 2019-04-12 |
Family
ID=66019724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910037226.1A Pending CN109609578A (en) | 2019-01-15 | 2019-01-15 | A method of the abstraction function albumen from inkfish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109609578A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113017064A (en) * | 2021-04-15 | 2021-06-25 | 东莞市保利德食品添加剂有限公司 | Preparation method of cuttlefish flavor seafood powder for quick-frozen food |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101245095A (en) * | 2008-01-10 | 2008-08-20 | 中国海洋大学 | Extraction of jellyfish collagen and method for producing collagen peptide |
| WO2010043073A1 (en) * | 2008-10-16 | 2010-04-22 | Shi Zongjie | Collagen polypeptide, preparation method and uses thereof |
| CN107897499A (en) * | 2017-12-20 | 2018-04-13 | 大连深蓝肽科技研发有限公司 | A kind of production method of inkfish oligopeptide |
| CN108191965A (en) * | 2018-02-08 | 2018-06-22 | 舟山海研食品科技有限公司 | Inkfish albumen and application thereof |
| CN108949882A (en) * | 2018-08-09 | 2018-12-07 | 浙江工商大学 | The method for extracting collagen peptide powder and hydroxyapatite |
-
2019
- 2019-01-15 CN CN201910037226.1A patent/CN109609578A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101245095A (en) * | 2008-01-10 | 2008-08-20 | 中国海洋大学 | Extraction of jellyfish collagen and method for producing collagen peptide |
| WO2010043073A1 (en) * | 2008-10-16 | 2010-04-22 | Shi Zongjie | Collagen polypeptide, preparation method and uses thereof |
| CN107897499A (en) * | 2017-12-20 | 2018-04-13 | 大连深蓝肽科技研发有限公司 | A kind of production method of inkfish oligopeptide |
| CN108191965A (en) * | 2018-02-08 | 2018-06-22 | 舟山海研食品科技有限公司 | Inkfish albumen and application thereof |
| CN108949882A (en) * | 2018-08-09 | 2018-12-07 | 浙江工商大学 | The method for extracting collagen peptide powder and hydroxyapatite |
Non-Patent Citations (1)
| Title |
|---|
| 姜元欣等: "几种动物蛋白水解液抗氧化活性比较研究", 《轻工科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113017064A (en) * | 2021-04-15 | 2021-06-25 | 东莞市保利德食品添加剂有限公司 | Preparation method of cuttlefish flavor seafood powder for quick-frozen food |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Alipal et al. | A review of gelatin: Properties, sources, process, applications, and commercialisation | |
| CN101061827B (en) | Industry method of producing fish collagen peptide from fish skin and bone by an enzyme method | |
| CN104140992B (en) | A kind of large-scale preparation method of fish scale Type I collagen protein peptides | |
| CN103445159B (en) | A kind of enzymatic-process preparation method of full constituent ginseng juice | |
| CN103992384A (en) | Pseudosciaena crocea fish bone collagen peptide, and preparation method and application thereof | |
| CN103060406B (en) | Method for Extracting Low Molecular Weight Collagen from Livestock Bone | |
| CN101904412B (en) | Method for producing food attractant by using fish soluble extract | |
| CN102276716A (en) | Method for extracting unmodified natural collagen from bullfrog skin | |
| CN103468773A (en) | Extraction method of walnut kernel polypeptide | |
| CN103243143A (en) | Method for extracting hydrolyzed collagen by using bone meal | |
| CN106174436A (en) | A kind of preparation method of complex enzyme hydrolysis fishbone hormone | |
| CN102154425B (en) | Production method of non-denatured II-type collagen | |
| CN104304647A (en) | Monkfish leftover classification using method | |
| CN107114793A (en) | It is a kind of to comprehensively utilize the method that sturgeon bone prepares calcium and chondroitin sulfate | |
| CN102732592A (en) | Method for preparing freshwater fish bone gelatin by enzyme process | |
| EP3621985A1 (en) | Methods for producing collagen | |
| CN102851341A (en) | Process for preparing collagen polypeptides through hydrolyzing codfish skins by double-enzyme composite method | |
| CN109609578A (en) | A method of the abstraction function albumen from inkfish | |
| CN107080263A (en) | A kind of bionic enzymatic prepares the production method of pea Gly-His-Lys | |
| CN109943616A (en) | A method of the abstraction function albumen from giant salamander | |
| CN102524797A (en) | Preparation method of pearl oyster peptide calcium | |
| CN105192242A (en) | Process for processing protein powder from corns | |
| CN104497122A (en) | Method for extracting gastric mucin from porcine gastric mucosa | |
| CN105002246A (en) | Fish polypeptide with uniform molecular weight distribution, and preparation method thereof | |
| WO2023082523A1 (en) | Method for improving extraction rate of chondroitin sulfate prepared from tilapia skull |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190412 |
|
| RJ01 | Rejection of invention patent application after publication |