CN105596391A - Application of Dendropanax chevaliert(Vig.)Merr.et Chun extract in preparing antimicrobial natural medicine and health-care food - Google Patents
Application of Dendropanax chevaliert(Vig.)Merr.et Chun extract in preparing antimicrobial natural medicine and health-care food Download PDFInfo
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- 230000000845 anti-microbial effect Effects 0.000 title claims abstract description 12
- 239000000284 extract Substances 0.000 title abstract description 20
- 241000202573 Dendropanax Species 0.000 title abstract description 5
- 235000013305 food Nutrition 0.000 title abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 37
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- 241000208340 Araliaceae Species 0.000 claims description 28
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 25
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- 229940079593 drug Drugs 0.000 claims description 7
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- AMJCTDBATIKENQ-YRDFTBLNSA-N aureusidin 6-O-beta-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C=C1O)=CC(O\2)=C1C(=O)C/2=C/C1=CC=C(O)C(O)=C1 AMJCTDBATIKENQ-YRDFTBLNSA-N 0.000 description 1
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- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention relates to application of Dendropanax chevaliert(Vig.)Merr.et Chun extract in preparing antimicrobial natural medicine and health-care food. An ethyl alcohol extracting solution or a methyl alcohol extracting solution of Dendropanax chevaliert(Vig.)Merr.et Chun is subjected to bacteriostatic activity research through a cup-plate method, it is found that the alcohol extracting solution of Dendropanax chevaliert(Vig.)Merr.et Chun has a strong inhibitory effect on staphylococcus arueus, Escherichia coli and aerogenesis Escherichia coli and serves as a premise and a foundation for screening and developing botanical fungicide, and a base is provided for preparing antimicrobial natural medicine or antimicrobial natural health-care food.
Description
Technical field
The present invention relates to a kind of functional study of setting conopsea extraction, be specifically related to set conopsea extraction to Escherichia coli,The colibacillary bacteriostasis of staphylococcus aureus and aerogenesis also relates to tree conopsea extraction in anti-microbial type simultaneouslyEffect in natural drug and health products or food.
Background technology
China's floristics is various, aboundresources, and the feature that environmental protection has no side effect makes its researching value high, sends outExhibition potentiality are large. The secondary metabolite of plant has exceeded 400,000 kinds, therefrom finds active strong plant source sterilizationAgent is an important topic of current drug research, is also one of developing direction of modern antibacterials.
Antibiotic and chemical synthetic drug are widely used in the many-sides such as human diseases, cultivation and plantation in recent yearsControl, effect highly significant, but long-term a large amount of use can make bacterium produce drug resistance and itself have poisonSide effect and human survival and environment are threatened. Chinese herbal medicine is the distinctive traditional Chinese medical theory of China and practiceProduct, it has medicinal, health care, nutrition effect such as have no side effect, and also has low toxicity, low-residual, resistance toThe many factors such as the low and aboundresources of property of medicine incidence, and be more and more subject to domestic and international researcher's attention.
Plant is the huge natural treasure-house of bioactive compound, at present both at home and abroad to the antibacterial research of plant veryActive. Tree ginseng, popular name dentiferous dendropanax root and stem, is distributed in the remote mountains area in the south of the River more, and happiness is in jungle concealment place growth, tree ginsengMedicinal the planting of one that (Dendropanaxdentiger (Harms) Merr.) is Araliaceae (Araliaceae)Thing, is Araliaceae aiphyllium, high 2-8 rice, and bark canescence, slightly level and smooth, spray cyan, wooden crisp;Dan Ye, helical form alternate, leaf variation is very large, is generally lotus leaf ellipse and leaf of pear tree ovum regardless of decomposite leaf sheetCircular, it is leaf that division blade presents maple, has the blade of three kinds of different shapes with one tree, umbel topRaw, total bennet is sturdy, long 1-3.5 centimetre, and fruit is outstanding achievement, is grown in dark and damp evergreen broadleaf forest,Mainly be distributed in each provinces and regions on the south the Changjiang river. Record according to " China's book on Chinese herbal medicine ": tree ginseng (Dendropanaxdentiger(Harms) Merr.) be usually used in rheumatism and rheumatoid arthritis, BN, paralyse, sprain, carbuncleThe treatment of the diseases such as furuncle, irregular menstruation, sequelae of infantile paralysis. Summary domestic and foreign literature, finds that tree ginseng is sameThe crude extract of platymiscium has very strong antibacterium, fungi, desinsection, anticancer, antiatherosclerosis, anti-The pharmacologically actives such as fatigue and anticomplement. But for tree ginseng, but have no it at mushrooms such as anti-Staphylococcus aureusIn application report.
Summary of the invention
The object of this invention is to provide a kind of tree conopsea extraction natural at preparation anti-microbial type natural drug and anti-microbial typeApplication in health products or food.
For reaching above-mentioned purpose, the technical solution adopted in the present invention is: a kind of tree conopsea extraction is at anti-bacteriaIn application, wherein, this tree conopsea extraction is ethanol or the methanol extract liquid of tree ginseng, this bacterium refers to large intestine barBacterium, staphylococcus aureus or aerogenesis Escherichia coli.
The present invention provides a kind of conopsea extraction answering in preparation anti-microbial type natural drug or health food of setting simultaneouslyWith, wherein, this bacterium refers to Escherichia coli, staphylococcus aureus or aerogenesis Escherichia coli, this tree conopsea extractionFor ethanol or the methanol extract liquid of tree ginseng.
The ethanol of above-mentioned tree ginseng or methanol extract liquid are to get dry tree ginseng to pulverize, and add by 1g tree ginseng powderThe ethanol of 20-50ml75%-95% mass concentration or the ratio of methanol solution add ethanol or methanol aqueous solution, soakBubble 24-72 hour, filters centrifugally, gets supernatant, and being concentrated into concentration with Rotary Evaporators is 0.5-2g/ml,Concentrate, through the degerming of miillpore filter device, obtains aseptic tree ginseng extract.
Wherein, described miillpore filter device is that aperture is the sterilizing filter of 0.22 μ m. The present invention adopts microporeFilter sterilization technique substitutes conventional autoclave sterilization method, can avoid destroying or change wherein effectivelyComposition.
The present invention adopts the ethanol of cylinder-plate method to tree ginseng or methanol extract liquid and ultra-pure water extract to carry out antibacterialActive research, finds that tree ginseng alcohol extract is to staphylococcus aureus, Escherichia coli and aerogenesis Escherichia coliInhibition stronger, tree ginseng water extraction liquid is stronger to staphylococcus aureus inhibition, is screening, exploitationThe prerequisite of botanical fungicide and basis, provide for preparing anti-microbial type natural drug or anti-microbial type natural health-care productsFoundation.
Detailed description of the invention
1. experiment equipment
For trying bacterial classification: Escherichia coli (Escherichiacoli), staphylococcus aureus(StaphyloccocusaureusRosenbach), aerogenesis Escherichia coli (coli-aerogenesgroup),For buying at bacterial classification center, Wuhan.
Test plant: tree ginseng (Dendropanaxdentiger (Harms) Merr.).
Laboratory apparatus: China of digital display thermostatical water bath (HH-4) state electrical equipment; Gas bath constant-temperature table (THZ-820)The prosperous instrument of state; Refrigerator (Rong Sheng BCD-185DKA); Su Jing is safe and sound for superclean bench (SW-CJ-1FD); RawChange incubator (SPX-250B-Z) Shanghai Bo Xun Industrial Co., Ltd.; Vertical pressure steam sterilizer (LS-50HJ)Jiangyin Binjiang Medical Equipment Co., Ltd.; Superpure water machine (UPWS-I-10T) Hangzhou forever clean reaching purifies limited public affairsDepartment; Electromagnetic oven (GCA16) Zhuhai Gree Household Electric Appliances Ltd etc.
LB fluid nutrient medium: peptone 10g, dusty yeast 5g, NaCl10g, pH value 7.0, add asepticWater is to 1000ml.
LB solid medium: peptone 10g, dusty yeast 5g, NaCl10g, agar 15-20g, pH value7.0, add sterilized water to 1000ml.
2. experimental technique
The preparation of tree ginseng water extraction liquid: take test plant dry product 20g, rub in rearmounted electromagnetic oven (GCA16),Add ultra-pure water 500ml, soak after 4h, boil 30min with little fire, filtration, centrifugal, obtain supernatant, residueAdd again 500ml poach 30min, centrifugal, get supernatant, merge twice filtrate, filtrate heating is concentrated into50ml, must set ginseng water extraction and get. Water extraction liquid is put HTHP in vertical pressure steam sterilizer (LS-50HJ)After (120 DEG C, 30min) sterilizing, put into superclean bench for subsequent use.
The preparation of tree ginseng alcohol extract: take test plant dry product 5g, after rubbing, put respectively in band plug wide-mouth bottle,Add 75% ethanol 100ml to soak 48h, centrifugal after filtration, it is (dense that filtrate is concentrated into 5ml with Rotary EvaporatorsDegree is 1g/ml), through miillpore filter, (m) degerming of 0.22 μ, must set ginseng alcohol extract to concentrate, puts into ultra-cleanWorkbench is for subsequent use.
The preparation of bacteria suspension: by dull and stereotyped setting-out method by staphylococcus aureus, Escherichia coli, aerogenesis large intestine barBacterium is inoculated in respectively on LB solid medium, seals with sealed membrane, puts for 37 DEG C and in biochemical cultivation case, cultivates 24hActivation bacterium, after activation, with the bacterium of the same bacterium colony of oese picking, is inoculated in LB fluid nutrient medium is housedIn vitro, be then placed in 37 DEG C of gas bath constant-temperature tables and cultivate 48h, make respectively the bacteria suspension of three kinds of bacterium,To be used as experimental strain.
3. bacteriostatic test
3.1. the mensuration of extract fungistatic effect
Adopt cylinder-plate method. Experimental strain is shaken up, pipettes 50ul bacteria suspension on agar plate with liquid-transfering gun,Tiling smoothens, 3 kinds of experimental bacteria, and every kind is coated with 10 flat boards, then sterilized Oxford cup is equidistantly placed inOn agar surface, 4, every ware, after all sequencing, quiet putting 10 minutes on super-clean bench. Use 1ml sterilizingLiquid-transfering gun is drawn tree ginseng aqueous extract 200ul, injects the Oxford cup setting, 3 Oxford cup notes in every wareIn-tree ginseng aqueous extract, the ultra-pure water 200ul that another Oxford cup injects sterilizing compares, and a kind of bacterium is cooked oneIndividual flat board, obtains 3 water extraction medicine flat boards. Equally, alcohol extract is also so (concentrated with 75% ethanol 100mlBe to contrast liquid to 5ml). Be placed in afterwards in 37 DEG C of biochemical cultivation cases and cultivate 24h, observe the bacterium in culture dishGrowing state, and with the diameter of miking inhibition zone. By the inhibition zone effect of a culture dish Chinese traditional medicineAverage, and data are accurate to one decimal place, result is as following table 1.
The fungistatic effect (unit: mm) of table 1 tree ginseng extract
| Tree ginseng extract | Staphylococcus aureus | Escherichia coli | Aerogenesis Escherichia coli |
| Water extraction liquid | 10.8 | 8.5 | 8.4 |
| Ultra-pure water contrast | 8.3 | 8.3 | 8.3 |
| Alcohol extract | 11.0 | 11.3 | 11.4 |
| Ethanol contrast | 9.2 | 9.2 | 9.2 |
As seen from the above table, tree ginseng water extraction liquid is stronger to staphylococcus aureus inhibition; Tree ginseng alcohol extract pairThe colibacillary fungistatic effect of staphylococcus aureus, Escherichia coli and aerogenesis is more remarkable.
3.2. the mensuration of variable concentrations extract to Bacteriostatic Effect
Adopt cylinder-plate method. Using extract as 100% stoste, the dilution of 2 times of methods of sterilized water, obtain 1:2,1:4,With 1:8 concentration extract dilution series. Because the extract of two kinds of methods respectively has the inhibition of bacteriumDifference, chooses respectively two kinds of obvious extracts of inhibition and does dilution experiment. Every a series ofly all distinguishConnect bacterium and compare, set Oxford cup and inject equal-volume medicine, putting 37 DEG C of constant temperature culture 24h, then measureSize the record of inhibition zone, and compare fungistatic effect, produces effect and be designated as "+", do not have effect to be designated as"-", the results are shown in following table 2.
Fungistatic effect (unit: mm) after the dilution of table 2 tree ginseng extract
| Tree ginseng extract | Staphylococcus aureus | Escherichia coli | Aerogenesis Escherichia coli |
| Water extraction liquid (1:2) | + | + | + |
| Water extraction liquid (1:4) | + | + | |
| Water extraction liquid (1:8) | － | － | － |
| Alcohol extract (1:2) | + | + | + |
| Alcohol extract (1:4) | + | + | + |
| Alcohol extract (1:8) | － | － | － |
As seen from the above table, tree ginseng extract wants to bring into play fungistatic effect, need to control finite concentration, and concentration tooLow meeting causes its bacteriostasis to bring into play and makes to think by mistake there is no fungistatic effect. Also can find out simultaneously,Same extract is roughly the same to the inhibitory action of several bacteriums, be as seen in extract, contain certain orIt is antibacterial that a common trait effect of growth, breeding or itself tissue of many kinds of substance to bacterium is brought into playEffect, this point awaits more deep research.
Claims (4)
1. set the application of conopsea extraction in anti-bacteria, wherein, this tree conopsea extraction is the second of tree ginsengAlcohol or methanol extract liquid, this bacterium refers to Escherichia coli, staphylococcus aureus or aerogenesis Escherichia coli.
2. set the application of conopsea extraction in preparation anti-microbial type natural drug or health food, wherein, shouldTree conopsea extraction is ethanol or the methanol extract liquid of tree ginseng, this bacterium refer to Escherichia coli, staphylococcus aureus orAerogenesis Escherichia coli.
3. application as claimed in claim 1 or 2, wherein, ethanol or the methanol extract liquid of described tree ginseng areGet dry tree ginseng and pulverize, add ethanol or the methyl alcohol of the 75%-95% mass concentration of 20-50ml by 1g tree ginseng powderThe ratio of solution adds ethanol or methanol solution, soaks 24-72 hour, filters centrifugally, gets supernatant, usesIt is 0.5-2g/ml that Rotary Evaporators is concentrated into concentration, and concentrate, through the degerming of miillpore filter device, obtains asepticTree ginseng extract.
4. application as claimed in claim 3, wherein, described miillpore filter device is that aperture is 0.22 μ mSterilizing filter.
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| CN201610053423.9A CN105596391A (en) | 2016-01-27 | 2016-01-27 | Application of Dendropanax chevaliert(Vig.)Merr.et Chun extract in preparing antimicrobial natural medicine and health-care food |
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Cited By (3)
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| CN106039391A (en) * | 2016-06-21 | 2016-10-26 | 林春梅 | Anti-bacterial high-viscosity medical glue and preparation method thereof |
| CN107334798A (en) * | 2017-07-18 | 2017-11-10 | 宜春学院 | Set conopsea extraction and preparation method and the application in rheumatoid arthritis medicine is treated |
| CN111808155A (en) * | 2020-08-24 | 2020-10-23 | 江西中医药大学 | Ginseng coumarin compound and application thereof in medicine |
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2016
- 2016-01-27 CN CN201610053423.9A patent/CN105596391A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106039391A (en) * | 2016-06-21 | 2016-10-26 | 林春梅 | Anti-bacterial high-viscosity medical glue and preparation method thereof |
| CN107334798A (en) * | 2017-07-18 | 2017-11-10 | 宜春学院 | Set conopsea extraction and preparation method and the application in rheumatoid arthritis medicine is treated |
| CN111808155A (en) * | 2020-08-24 | 2020-10-23 | 江西中医药大学 | Ginseng coumarin compound and application thereof in medicine |
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