CN105595030A - Biologically prepared mildew preventive and preparation process thereof - Google Patents
Biologically prepared mildew preventive and preparation process thereof Download PDFInfo
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- CN105595030A CN105595030A CN201610173744.2A CN201610173744A CN105595030A CN 105595030 A CN105595030 A CN 105595030A CN 201610173744 A CN201610173744 A CN 201610173744A CN 105595030 A CN105595030 A CN 105595030A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 230000003449 preventive effect Effects 0.000 title abstract description 5
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 69
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 69
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 69
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims abstract description 54
- 239000003921 oil Substances 0.000 claims abstract description 54
- 235000019198 oils Nutrition 0.000 claims abstract description 54
- 229940083466 soybean lecithin Drugs 0.000 claims abstract description 54
- 239000012141 concentrate Substances 0.000 claims abstract description 50
- 235000021388 linseed oil Nutrition 0.000 claims abstract description 37
- 239000000944 linseed oil Substances 0.000 claims abstract description 37
- 239000000843 powder Substances 0.000 claims abstract description 32
- 239000000706 filtrate Substances 0.000 claims abstract description 19
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 18
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 18
- 239000012528 membrane Substances 0.000 claims abstract description 17
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 16
- 239000008103 glucose Substances 0.000 claims abstract description 16
- 239000000047 product Substances 0.000 claims abstract description 16
- 238000003756 stirring Methods 0.000 claims abstract description 16
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims abstract description 15
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 15
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims abstract description 15
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000012530 fluid Substances 0.000 claims description 79
- 239000003112 inhibitor Substances 0.000 claims description 62
- 235000015097 nutrients Nutrition 0.000 claims description 47
- 238000004108 freeze drying Methods 0.000 claims description 30
- 229920002678 cellulose Polymers 0.000 claims description 13
- 239000001913 cellulose Substances 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 10
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims description 2
- 230000036528 appetite Effects 0.000 abstract description 2
- 235000019789 appetite Nutrition 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 abstract description 2
- 238000001728 nano-filtration Methods 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract 3
- 238000009630 liquid culture Methods 0.000 abstract 3
- 238000000855 fermentation Methods 0.000 abstract 2
- 230000004151 fermentation Effects 0.000 abstract 2
- 241000282887 Suidae Species 0.000 abstract 1
- 235000021052 average daily weight gain Nutrition 0.000 abstract 1
- 235000021050 feed intake Nutrition 0.000 abstract 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 12
- 230000000694 effects Effects 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 6
- 239000004310 lactic acid Substances 0.000 description 6
- 235000014655 lactic acid Nutrition 0.000 description 6
- 235000019260 propionic acid Nutrition 0.000 description 6
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 6
- 235000014590 basal diet Nutrition 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 230000037406 food intake Effects 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000009931 harmful effect Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 108010062877 Bacteriocins Proteins 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 229920001202 Inulin Polymers 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 201000010538 Lactose Intolerance Diseases 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000004426 flaxseed Nutrition 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 229940029339 inulin Drugs 0.000 description 1
- -1 inulin oligosaccharides Chemical class 0.000 description 1
- 150000002561 ketenes Chemical class 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3571—Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3562—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a biologically prepared mildew preventive and a preparation process thereof. The preparation process comprises the following steps: S1, putting lactobacillus acidophilus freeze-dried powder into a liquid culture medium for cultivation under the temperature of 37 DEG C for 20 to 28 hours until the pH value reaches 3.6 to 4.0 to obtain lactobacillus acidophilus fermentation liquor, wherein every liter of the liquid culture medium contains 80 to 120 grams of bean pulp, 20 to 40 grams of glucose and the balance of water, and the weight of the lactobacillus acidophilus freeze-dried powder is 1 to 3 percent of the volume of the liquid culture medium; S2, filtering the lactobacillus acidophilus fermentation liquor with a nanofiltration membrane with the molecular weight cut-off of 8,000 to 12,000 Da, and collecting a filtrate; and S3, circularly concentrating the filtrate 8 to 12 times to obtain a concentrate, adding soybean lecithin oil, linseed oil and carboxymethylcellulose, uniformly stirring, and then homogenizing with a homogenizing machine for 2 to 4 times to obtain the mildew preventive finished product, wherein the volume of the soybean lecithin oil is 2 to 4 percent of the volume of the concentrate; the volume ratio of the soybean lecithin oil to the linseed oil is (3-5): 1; and the weight of carboxymethylcellulose is 0.4 to 0.6 percent of the volume of the concentrate. After the mildew preventive is added into a basic ration, the appetite of weaned pigs can be improved, and the average daily feed intake and the average daily weight gain can be increased.
Description
Technical field
The present invention relates to mould inhibitor, be specifically related to a kind of mould inhibitor and preparation technology thereof of Biological preparation.
Background technology
Feed is in process, and after various cereal is pulverized, its nutritional labeling is exposed in air, again because raisingMaterial raw material be crops, these crops results in a large amount of moulds or mycotic spore,In the process of feed, these moulds can not be killed, complete while depositing feed is processed, ifIt is improper to preserve, and feed very easily goes mouldy, and the mycotoxin content going mouldy in feed is very high, can produce livestock and poultryA series of harmful effect, brings various health problems to domestic animal, and the most directly performance is exactly under animal feed intakeFall; Immune programme for children is constant, and vaccine is inoculated on time, but the antibody horizontal of livestock and poultry do not increase all the time, under immunityFall, the production performance of livestock and poultry reduces, and when ketenes content overproof as red in corn in feed, easily causes the vacation of sowReturn feelings; Therefore need to add mould inhibitor in feed. General mould inhibitor is taking organic acid as main, as propionic acid, benzene firstAcid, fumaric acid etc., anti-mold effect is poor.
Lactic acid bacteria is the main fungal component of animal alimentary canal, can form normal flora, at micro-aerobic or anaerobic conditionLower generation lactic acid, has stronger acid resistance, can produce one or more bacteriocins, can effectively suppress hay bacillus,The growth of the harmful bacteria such as Escherichia coli, staphylococcus. Its shortcoming is that temperature capacity is poor, 65～75 DEG C of conditionsLower dead quantity is more. Apply also the most extensively the most the earliest, at present the lactic acid bacteria of main application has lactobacillus acidophilus, twoDiscrimination lactobacillus and enterococcus. Lactic acid bacteria as feed addictive should possess stronger acid producing ability, is beneficial to disappearChange digesting and assimilating of road nutriment; It is high that selected bacterial strain breeding potential is wanted, and could occupy rapidly like this alimentary canal; AlsoShould possess the ability of surviving in the sour environment of stomach.
Lactobacillus acidophilus is lactic acid bacteria important in human body intestinal canal, can regulate human body enteric microorganism colony balance,Strengthen immunity of organisms, reduce cholesterol, alleviate the formation of lactose intolerance, inhibition tumor cell, be conducive toControl adult and children's diarrhae etc., particularly have important to maintaining intestines and stomach normal physiological function to healthEffect, evidence show and in milk, add inulin oligosaccharides can significantly promote baby intestinal bifid bacterium and lactic acid bacteriaBreeding, nutritionally can reach the merit effect identical with people's milk.
At present, there is not yet the report of preparing Midew preventive for feed with lactobacillus acidophilus.
Summary of the invention
The object of the present invention is to provide a kind of mould inhibitor and preparation technology thereof of Biological preparation.
Above-mentioned purpose is to be achieved by technical scheme below:
A mould inhibitor for Biological preparation, is prepared from by the following method:
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 20～28h for 37 DEG C, to pHValue is 3.6～4.0, obtains lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 80～120g dregs of beans and 20～40gGlucose, surplus is water; The weight of lactobacillus acidophilus freeze-drying powder is 1～3% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 8000～12000Da with molecular cut off is filtered,Collect filtrate;
Step S3, filtrate cycle concentrates 8～12 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylicMethylcellulose, crosses 2～4 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volumeFor 2～4% of concentrate volume, soybean lecithin oil is 3～5:1 with the volume ratio of linseed oil, carboxymethyl celluloseElement weight is 0.4～0.6% of concentrate volume.
Further, in step S1, to pH value 3.8.
Further, in step S1, every 1L fluid nutrient medium contains 100g dregs of beans and 30g glucose.
Further, in step S2, the nanofiltration that lactobacillus acidophilus zymotic fluid is 10000Da with molecular cut offMembrane filtration.
Further, in step S3, soybean lecithin oil is 4:1 with the volume ratio of linseed oil.
Further, the mould inhibitor of described Biological preparation is prepared from by the following method:
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 24h for 37 DEG C, to pH value is3.8, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 100g dregs of beans and 30g glucose, more thanAmount is water; The weight of lactobacillus acidophilus freeze-drying powder is 2% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 10000Da with molecular cut off is filtered, and collectsFiltrate;
Step S3, filtrate cycle concentrates 10 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 3 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 3%, soybean lecithin oil is 4:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.5% of concentrate volume.
Further, the mould inhibitor of described Biological preparation is prepared from by the following method:
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 20h for 37 DEG C, to pH value is4.0, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 80 dregs of beans and 20 glucose, and surplus isWater; The weight of lactobacillus acidophilus freeze-drying powder is 1% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 8000Da with molecular cut off is filtered, and collects filterLiquid;
Step S3, filtrate cycle concentrates 8 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 2 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 2%, soybean lecithin oil is 3:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.4% of concentrate volume.
Further, the mould inhibitor of described Biological preparation is prepared from by the following method:
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 28h for 37 DEG C, to pH value is3.6, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 120g dregs of beans and 40g glucose, more thanAmount is water; The weight of lactobacillus acidophilus freeze-drying powder is 3% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 12000Da with molecular cut off is filtered, and collectsFiltrate;
Step S3, filtrate cycle concentrates 12 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 4 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 4%, soybean lecithin oil is 5:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.6% of concentrate volume.
The using method of above-mentioned mould inhibitor, adds mould inhibitor described in 1～5kg in feed per ton.
Further, in feed per ton, add mould inhibitor described in 3kg.
Advantage of the present invention:
After mould inhibitor provided by the invention adds in basal diet, can improve the appetite of weanling pig, significantly carryHigh average daily ingestion amount, average daily gain significantly improves, and illustrates that mould inhibitor of the present invention can prevent the mouldy change of feedMatter. The effect of mould inhibitor of the present invention is better than propionic acid type mould inhibitor conventional in prior art.
Detailed description of the invention
Further illustrate essentiality content of the present invention below in conjunction with embodiment, protect but do not limit the present invention with thisProtect scope. Although the present invention is explained in detail with reference to preferred embodiment, those of ordinary skill in the art shouldWork as understanding, can modify or be equal to replacement technical scheme of the present invention, and not depart from the technology of the present inventionThe essence of scheme and scope.
Embodiment 1: the preparation of mould inhibitor
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 24h for 37 DEG C, to pH value is3.8, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 100g dregs of beans and 30g glucose, more thanAmount is water; The weight of lactobacillus acidophilus freeze-drying powder is 2% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 10000Da with molecular cut off is filtered, and collectsFiltrate;
Step S3, filtrate cycle concentrates 10 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 3 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 3%, soybean lecithin oil is 4:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.5% of concentrate volume.
Embodiment 2: the preparation of mould inhibitor
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 20h for 37 DEG C, to pH value is4.0, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 80 dregs of beans and 20 glucose, and surplus isWater; The weight of lactobacillus acidophilus freeze-drying powder is 1% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 8000Da with molecular cut off is filtered, and collects filterLiquid;
Step S3, filtrate cycle concentrates 8 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 2 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 2%, soybean lecithin oil is 3:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.4% of concentrate volume.
Embodiment 3: the preparation of mould inhibitor
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 28h for 37 DEG C, to pH value is3.6, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 120g dregs of beans and 40g glucose, more thanAmount is water; The weight of lactobacillus acidophilus freeze-drying powder is 3% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 12000Da with molecular cut off is filtered, and collectsFiltrate;
Step S3, filtrate cycle concentrates 12 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 4 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 4%, soybean lecithin oil is 5:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.6% of concentrate volume.
Embodiment 4: the preparation of mould inhibitor
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 24h for 37 DEG C, to pH value is3.8, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 100g dregs of beans and 30g glucose, more thanAmount is water; The weight of lactobacillus acidophilus freeze-drying powder is 2% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 10000Da with molecular cut off is filtered, and collectsFiltrate;
Step S3, filtrate cycle concentrates 10 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 3 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 3%, soybean lecithin oil is 3:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.5% of concentrate volume.
Embodiment 5: the preparation of mould inhibitor
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 24h for 37 DEG C, to pH value is3.8, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 100g dregs of beans and 30g glucose, more thanAmount is water; The weight of lactobacillus acidophilus freeze-drying powder is 2% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 10000Da with molecular cut off is filtered, and collectsFiltrate;
Step S3, filtrate cycle concentrates 10 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 3 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 3%, soybean lecithin oil is 5:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.5% of concentrate volume.
The contrast of embodiment 6: embodiment 1, soybean lecithin oil is 2:1 with the volume ratio of linseed oil
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 24h for 37 DEG C, to pH value is3.8, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 100g dregs of beans and 30g glucose, more thanAmount is water; The weight of lactobacillus acidophilus freeze-drying powder is 2% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 10000Da with molecular cut off is filtered, and collectsFiltrate;
Step S3, filtrate cycle concentrates 10 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 3 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 3%, soybean lecithin oil is 2:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.5% of concentrate volume.
The contrast of embodiment 7: embodiment 1, soybean lecithin oil is 6:1 with the volume ratio of linseed oil
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 24h for 37 DEG C, to pH value is3.8, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 100g dregs of beans and 30g glucose, more thanAmount is water; The weight of lactobacillus acidophilus freeze-drying powder is 2% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 10000Da with molecular cut off is filtered, and collectsFiltrate;
Step S3, filtrate cycle concentrates 10 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 3 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 3%, soybean lecithin oil is 6:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.5% of concentrate volume.
Embodiment 8: effect embodiment, adds the effect experiment of different mould inhibitors and ties in weanling pig daily ration
Fruit contrast
The anti-mold effect of test implementation example 1, embodiment 4～7 mould inhibitors, and with do not add mould inhibitor basal diet,The test group of adding propionic acid type mould inhibitor contrasts. Addition in feed: mould inhibitor of the present invention, feed per tonMiddle interpolation 3kg mould inhibitor; Propionic acid type mould inhibitor, adds 5kg in feed per ton.
| Test article | Average daily ingestion amount (g) | Average daily gain (g) |
| Basal diet | 1088±52 | 475±34 |
| Embodiment 1 | 1134±47 | 525±37 |
| Embodiment 4 | 1131±45 | 520±39 |
| Embodiment 5 | 1132±53 | 522±33 |
| Embodiment 6 | 1092±57 | 487±38 |
| Embodiment 7 | 1091±50 | 485±30 |
| Propionic acid type mould inhibitor | 1099±54 | 502±35 |
Embodiment 2 and embodiment 3 have the effect close with embodiment 1.
The above results shows, after mould inhibitor provided by the invention adds in basal diet, can improve weanling pigAppetite, significantly improve average daily ingestion amount, average daily gain significantly improves, and illustrates that mould inhibitor of the present invention is passablePrevent feed moldy metamorphism. The effect of mould inhibitor of the present invention is better than propionic acid type mould inhibitor conventional in prior art.
The effect of mould inhibitor of the present invention probably with preparation method in the volume ratio of soybean lecithin oil and linseed oilRelevant, when soybean lecithin oil is 3～5:1 with the volume ratio of linseed oil, the mould inhibitor of Biological preparation of the present inventionEffect is best.
The effect of above-described embodiment is to illustrate essentiality content of the present invention, but does not limit of the present invention with thisProtection domain. Those of ordinary skill in the art should be appreciated that and can modify to technical scheme of the present inventionOr be equal to replacement, and do not depart from essence and the protection domain of technical solution of the present invention.
Claims (10)
1. a mould inhibitor for Biological preparation, is characterized in that, is prepared from by the following method:
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 20～28h for 37 DEG C, to pHValue is 3.6～4.0, obtains lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 80～120g dregs of beans and 20～40gGlucose, surplus is water; The weight of lactobacillus acidophilus freeze-drying powder is 1～3% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 8000～12000Da with molecular cut off is filtered,Collect filtrate;
Step S3, filtrate cycle concentrates 8～12 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylicMethylcellulose, crosses 2～4 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volumeFor 2～4% of concentrate volume, soybean lecithin oil is 3～5:1 with the volume ratio of linseed oil, carboxymethyl celluloseElement weight is 0.4～0.6% of concentrate volume.
2. the mould inhibitor of Biological preparation according to claim 1, is characterized in that: in step S1,To pH value 3.8.
3. the mould inhibitor of Biological preparation according to claim 2, is characterized in that: in step S1,Every 1L fluid nutrient medium contains 100g dregs of beans and 30g glucose.
4. the mould inhibitor of Biological preparation according to claim 3, is characterized in that: in step S2,The NF membrane that lactobacillus acidophilus zymotic fluid is 10000Da with molecular cut off is filtered.
5. the mould inhibitor of Biological preparation according to claim 4, is characterized in that: in step S3,Soybean lecithin oil is 4:1 with the volume ratio of linseed oil.
6. the mould inhibitor of Biological preparation according to claim 5, is characterized in that, by as belowMethod is prepared from:
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 24h for 37 DEG C, to pH value is3.8, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 100g dregs of beans and 30g glucose, more thanAmount is water; The weight of lactobacillus acidophilus freeze-drying powder is 2% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 10000Da with molecular cut off is filtered, and collectsFiltrate;
Step S3, filtrate cycle concentrates 10 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 3 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 3%, soybean lecithin oil is 4:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.5% of concentrate volume.
7. the mould inhibitor of Biological preparation according to claim 1, is characterized in that, by as belowMethod is prepared from:
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 20h for 37 DEG C, to pH value is4.0, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 80 dregs of beans and 20 glucose, and surplus isWater; The weight of lactobacillus acidophilus freeze-drying powder is 1% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 8000Da with molecular cut off is filtered, and collects filterLiquid;
Step S3, filtrate cycle concentrates 8 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 2 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 2%, soybean lecithin oil is 3:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.4% of concentrate volume.
8. the mould inhibitor of Biological preparation according to claim 1, is characterized in that, by as belowMethod is prepared from:
Step S1, gets lactobacillus acidophilus freeze-drying powder in fluid nutrient medium, cultivates 28h for 37 DEG C, to pH value is3.6, obtain lactobacillus acidophilus zymotic fluid; Every 1L fluid nutrient medium contains 120g dregs of beans and 40g glucose, more thanAmount is water; The weight of lactobacillus acidophilus freeze-drying powder is 3% of fluid nutrient medium volume;
Step S2, the NF membrane that lactobacillus acidophilus zymotic fluid is 12000Da with molecular cut off is filtered, and collectsFiltrate;
Step S3, filtrate cycle concentrates 12 times and obtains concentrate, adds soybean lecithin oil, linseed oil and carboxylic firstBase cellulose, crosses 4 times homogenizers and obtains mould inhibitor finished product after stirring; Described soybean lecithin oil volume is denseContracting liquid long-pending 4%, soybean lecithin oil is 5:1 with the volume ratio of linseed oil, carboxymethyl cellulose weight is0.6% of concentrate volume.
9. the using method of the arbitrary described mould inhibitor of claim 1～8, is characterized in that: in feed per ton, addAdd mould inhibitor described in 1～5kg.
10. using method according to claim 9, is characterized in that: in feed per ton, add 3kg instituteState mould inhibitor.
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| CN106261476A (en) * | 2016-08-16 | 2017-01-04 | 梅庆波 | A kind of preparation method of feeding mildew removing agent |
| CN108902649A (en) * | 2018-07-27 | 2018-11-30 | 苏州汉德瑞生物工程有限公司 | A kind of preparation method of microbial food mould inhibitor |
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| CN102326677A (en) * | 2011-10-21 | 2012-01-25 | 北京大北农科技集团股份有限公司 | Composite micro-ecological feed additive and preparation method and premix thereof |
| CN103976147A (en) * | 2014-05-30 | 2014-08-13 | 青岛科奈尔饲料有限公司 | Lactobacillus acidophilus pomace fermented feed and production process thereof |
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| CN102058016A (en) * | 2009-11-17 | 2011-05-18 | 程显东 | Efficient feed mildew inhibitor |
| CN102229902A (en) * | 2011-05-27 | 2011-11-02 | 北京大北农科技集团股份有限公司 | Bacillus pumilus, feed additive thereof, premix, and feed mildewproof agent |
| CN102326677A (en) * | 2011-10-21 | 2012-01-25 | 北京大北农科技集团股份有限公司 | Composite micro-ecological feed additive and preparation method and premix thereof |
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Application publication date: 20160525 |