CN105586259A - Control system and control method for cell image acquisition device in cell culture - Google Patents
Control system and control method for cell image acquisition device in cell culture Download PDFInfo
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Abstract
The invention relates to the technical field of cell culture, in particular to a control system for a cell image acquisition device in cell culture. The control system comprises a data processing module, a first motion control module, a second motion control module, a flashlight frequency control module, a camera mechanism shutter control module and a displacement detection module, wherein the first motion control module controls a flashlight module to move; the second motion control module controls a camera mechanism to move; the camera mechanism shutter control module controls opening and closing of a shutter of the camera mechanism; the flashlight frequency control module controls flash time of a flashlight; the displacement detection module detects motion displacement of the flashlight module and camera mechanism; the data processing module sends a motion signal to the first motion control module and the second motion control module, and whether the shutter is opened and whether the flashlight is on are determined. The flashlight and the camera mechanism are controlled by the control system to move synchronously, and the control system has the benefit that images of cells in a cell culture plate are acquired effectively in a continuous moving process.
Description
Technical field
The present invention relates to cell culture technology field, particularly in a kind of cell cultivation, cell image obtainsGet control system and the method for device.
Background technology
In the process that cell is cultivated, need to obtain cellular morphology and change, generally adopt at present image mechanismTake pictures to obtain the method for cell morphology image in culture dish, due to image resolution ratio requirement, video cameraStructure once can only be taken the image of the subregion in culture dish, need to repeatedly absorb just can complete wholeIn culture dish, cell morphology image obtains.
In traditional cell culture system for the obtaining of cell morphology image in culture dish, be take generalLogical light source, certain position that first light source and image mechanism is synchronized with the movement, then stop and take pictures, then move,Stop, taking pictures, complete to the obtaining of whole culture dish cell morphology image, like this by do actionThe motion blur problems that can effectively avoid moving camera shooting to occur. But, this traditional mode one sideFace can make the process of taking pictures very slow; On the other hand, in this process light source in opening always,And light source and camera lens produce vibrations in stopped process, thereby produce displacement error, make image notComplete.
Therefore, need to realize a kind of and take the mode of taking pictures in persistent movement process, easily produce fortuneDynamic model is stuck with paste, and what make to take is not fogging clear. And taking pictures in motion process continuously, how to control fortuneMoving, shutter speed and flash lamp flashing rate, realize blur-free imaging in continuous motion process, is orderBefore need the problem of solution badly.
Summary of the invention
The present invention is intended to overcome and in existing cell culture system, obtains cell and cultivate that the efficiency of image is low, figureThe technological deficiency that image quality is poor, provide a kind of cell cultivate in the control system of cell image acquisition device,Comprise data processing module, the first motion-control module, the second motion-control module, flash lamp frequencyControl module, image mechanism shutter control module and displacement detection module;
Described the first motion-control module, for according to the signal of described data processing module, controls and dodgesLight modulation block motion;
Described the second motion-control module, for according to the signal of described data processing module, control is taken the photographPicture mechanism kinematic;
Described image mechanism shutter control module, for according to the signal of described data processing module, controlsThe opening and closing of image mechanism shutter processed;
Described flash lamp frequency control module, for according to the signal of described data processing module, controlsThe flash time of flash lamp;
Described displacement detection module, for detection of the motion of described flash modules and described image mechanismDisplacement, and send displacement information to described data processing module;
Described data processing module, for sending respectively motor message to the first motion-control module andTwo motion-control modules, are synchronized with the movement described flash modules and described image mechanism; Also useIn described displacement information is processed, judge whether to open shutter and flash lamp; Also forSend shutter opening signal to described image mechanism, send flash lamp start signal to described flash lamp mouldPiece.
In some embodiment, described flash modules is arranged on the first slide rail by slide block, described in take the photographCamera structure is arranged on the second slide rail by slide block, and described flash modules and described image mechanism are respectivelyBe positioned at the both sides up and down of Tissue Culture Plate.
In some embodiment, described cell image acquisition device also comprises motor, described the first motion controlImage mechanism and described sudden strain of a muscle described in driven by motor described in molding piece and described the second moving control module for controllingLight modulation module is synchronized with the movement.
In some embodiment, described data processing module is the machine platform controller during cell is cultivated.
In some embodiment, described cell image acquisition device also comprises grating scale, described grating scale edgeDescribed the first slide rail and described the second slide rail be arranged in parallel, and described displacement detection module detects described flash of lightThe distance that lamp module and described image mechanism move along described grating scale, and send a signal to described dataProcessing module.
In some embodiment, described control system is also for detecting according to described displacement detection moduleMotor message calculate and adjust the movement velocity of described flash modules and described image mechanism.
In some embodiment, the shutter of described image mechanism is 10 milliseconds, the sudden strain of a muscle of described flash modulesIt between the light time, is microsecond rank.
Correspondingly, the present invention also provides the control method of cell image acquisition device in a kind of cell cultivation,Comprise the following steps:
S1, data processing module send respectively motor message to the first motion-control module and the second motionControl module, is synchronized with the movement flash modules and image mechanism;
S2, described the first motion-control module, according to the signal of described data processing module, are controlled flash of lightLamp module motion; Described the second motion-control module, according to the signal of described data processing module, is controlledThe motion of image mechanism;
S3, displacement detection module detect the moving displacement of described flash modules and described image mechanism,And send displacement information to described data processing module;
S4, described data processing module are processed displacement information, judge whether current motion distanceFrom the position that whether needs to open shutter and flash lamp, if so, perform step S5; If not, returnReturn step S3;
S5, described data processing module send shutter opening signal to described image mechanism, send flash of lightLamp start signal is to flash modules;
The opening and closing of S6, described image mechanism shutter control module controls image mechanism shutter; InstituteState flash lamp frequency control module control flash lamp and open flash of light, and control flash time.
In some embodiment, described data processing module is the cell training at cell image acquisition device placeMachine platform controller in the system of supporting.
In some embodiment, in described step S2, described the first motion-control module and described the second fortuneMoving control module control driven by motor image mechanism 3 and flash modules are synchronized with the movement.
In some embodiment, in described step S3, described displacement detection module detects described flash lamp mouldThe distance that piece and described image mechanism move along grating scale, and send a signal to described data processing module.
Beneficial effect of the present invention is: of the present invention by data processing module, motion-control moduleAnd flash lamp frequency control module and image mechanism shutter control module, control flash lamp and video camera enoughBe synchronized with the movement, and flashing rate and the shutter opening time of flash lamp are set according to movement velocity, therebyReach the beneficial effect that effectively obtains cell image in Tissue Culture Plate in continuous motion process.
More, the present invention, by displacement detection module and grating scale structure are set, has reached effective rootAdjust the technique effect of flash lamp frequency according to movement velocity.
Brief description of the drawings
Fig. 1 is the structure chart of cell image acquisition device during cell provided by the invention is cultivated;
Fig. 2 is the control system module map of cell image acquisition device during cell provided by the invention is cultivated;
Fig. 3 is the control method flow chart of cell image acquisition device during cell provided by the invention is cultivated.
Illustration:
1, flash lamp 2, the first slide rail
3, image mechanism 4, the second slide rail
5, control system 6, slide block
7, grating scale 8, displacement detection module
9, Tissue Culture Plate 51, data processing module
52, the first motion-control module 53, the second motion-control module
54, flash lamp frequency control module 55, image mechanism shutter control module
56, displacement detection module
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with accompanying drawingAnd specific embodiment, the present invention is further elaborated. Should be appreciated that described hereinSpecific embodiment is only in order to explain the present invention, and is not construed as limiting the invention.
Technical problem to be solved by this invention be how to realize a kind of can be in the process of continuous fortune meritObtain cell clearly and cultivate image.
The present invention is on the basis of cell image acquisition device in cell is cultivated, and its control system is enteredRow is further set forth, therefore, and before describing the concrete structure of control system, in first cell being cultivatedCell image acquisition device is described. Refer to Fig. 1, show cell image in cell cultivation and obtainThe structure chart of a specific embodiment of device. Specifically comprise flash lamp 1, image mechanism 3, control system5, and be parallel to each other the first slide rail 2 and the second slide rail 4 placed. Flash lamp 1 is stroboscopic light,Flash lamp 1 is arranged on the first slide rail 2 by slide block 6, and flash lamp 1 is positioned at Tissue Culture Plate 9Top, can move with respect to Tissue Culture Plate 9 along the first slide rail 2, for the flash of light of the process of taking picturesIllumination. The below (bottom surface) of Tissue Culture Plate 9 arranges image mechanism 3, and image mechanism 3 passes through slide blockBe arranged on the second slide rail 4. The first slide rail 2 devices and the second slide rail 4 install respectively (schemes with motorIn not shown) connect. Control system 5 sends a signal to motor, thereby controls flash lamp 1 and shootingMechanism 3 transports with respect to Tissue Culture Plate 9 synchronous uniform velocities along the first slide rail 2 and the second slide rail 4 respectivelyMoving. The movement velocity of flash lamp 1 and image mechanism 3 is that control system 5 sets in advance. Flash lamp 1Flashing rate be that control system 5 arranges according to the movement velocity of flash lamp 1. Stroboscopic light 1Movement velocity and flashing rate (1/ flash time) need to meet the following conditions: flash time * motionThe motion blur distance of the speed≤permission of taking pictures.
When work, control system 5 sends a signal to motor, Electric Machine Control slide block drive flash lamp 1 andImage mechanism 2 is at the uniform velocity transported with respect to Tissue Culture Plate 9 along the first slide rail 2 and the second slide rail 4 respectivelyMoving speed. Control system 5 is controlled the shutter opening of image mechanism 3, and controls flash lamp 1 in shootingDuring mechanism's 3 shutter openings, glisten, thereby obtain the cell morphology image on Tissue Culture Plate.
As preferably, described image mechanism is microlens, and shutter is 10 milliseconds the soonest, and flash lamp dodgesIt between the light time, is microsecond rank.
As preferably, the flasher in cell culture system also comprises with described image mechanism 3 and being connectedStorage device, for storing described cellular morphology picture.
In embodiments of the invention, cell culture system high speed flasher also comprises grating scale 7 HesDisplacement detection module 8, grating scale 7 be arranged in parallel along described the first slide rail 2 and described the second slide rail 4,Grating scale 7 is the movement position along slide rail for Calibrating Flashlamp 1 and image mechanism 3. Displacement detecting mouldPiece 8 is the move distance along described grating scale 7 for detection of flash lamp 1 and image mechanism 3, displacement inspectionSurvey module 8. Control system 5 is also for receiving the signal of described displacement detection module 8, and according to displacementThe detected motor message of detection module 8 calculates and adjusts the flashing rate motion speed of described flash lampDegree. Therefore, the present invention, by displacement detection module and grating scale structure are set, has reached effective basisMovement velocity is adjusted the technique effect of flash lamp frequency.
The present invention is enough synchronized with the movement by control system control flash lamp and video camera, and according to motion speedDegree arranges the flashing rate of flash lamp, effectively obtains Tissue Culture Plate thereby reach in continuous motion processThe effect of middle cell image. Please participate in Fig. 2, show cell image in cell cultivation of the present invention and obtain dressThe control system module map of putting. This control system comprises data processing module 51, the first motion control mouldPiece 52, the second motion-control module 53, flash lamp frequency control module 54, image mechanism shutter controlModule 55 and displacement detection module 56. Specific works process is:
Data processing module 51 sends respectively motor message to the first motion-control module 52 and the second fortuneMoving control module 53, is synchronized with the movement flash modules 1 and image mechanism 3. Preferably, numberIt is the machine platform controller in the cell culture system at cell image acquisition device place according to processing module 51.
The first motion-control module 52, according to the signal of data processing module 51, is controlled flash modules 1Motion; The second motion-control module 53, according to the signal of data processing module 51, is controlled image mechanism 3Motion. The first motion-control module 52 and the second motion-control module 53 are controlled driven by motor shootingMechanism 3 and flash modules 1 are synchronized with the movement.
Displacement detection module 56 detects the moving displacement of flash modules 1 and image mechanism 3, and sendsDisplacement information is to data processing module 51. Particularly, displacement detection module 56 detects flash modules 1And image mechanism 3 distance of moving along grating scale 7, and send a signal to described data processing module 51.
Data processing module 51 is processed displacement information, whether judges whether current move distanceReach the position of opening shutter and flash lamp. If reach, send shutter opening signal to image mechanism 3,Send flash lamp start signal to flash modules 1.
Image mechanism shutter control module 55, according to the signal of data processing module 51, is controlled video cameraThe opening and closing of structure 3 shutters. Flash lamp frequency control module 54 is according to 51 of data processing modulesThe signal sending, controls flash lamp and opens flash of light, and control flash time. By control system to cellThe control of cell image acquisition device in cultivation, thereby the obtaining of cell image of realizing culture plate.
Refer to Fig. 3, the present invention also provides the controlling party of cell image acquisition device in a kind of cell cultivationMethod, specifically comprises the following steps:
Execution step S1, data processing module send respectively motor message to the first motion-control module andThe second motion-control module, is synchronized with the movement flash modules and image mechanism. Described data placeReason module is the machine platform controller in the cell culture system at cell image acquisition device place.
Execution step S2, described the first motion-control module is according to the signal of described data processing module,The motion of control flash modules; Described the second motion-control module is according to the letter of described data processing moduleNumber, control the motion of image mechanism. Described the first motion-control module and described the second motion control mouldPiece control driven by motor image mechanism 3 and flash modules 1 are synchronized with the movement.
Execution step S3, displacement detection module detects the fortune of described flash modules and described image mechanismMoving displacement, and send displacement information to described data processing module. Described displacement detection module detects instituteState the distance that flash modules and described image mechanism move along grating scale, and send a signal to described numberAccording to processing module.
Execution step S4, described data processing module is processed displacement information, judges whether to work asWhether front move distance needs to open the position of shutter and flash lamp, and if not, displacement detection module continuesContinuous detection; If so, described data processing module sends shutter opening signal to described image mechanism,Send flash lamp start signal to flash modules 1.
Execution step S6, the unlatching of described image mechanism shutter control module controls image mechanism shutter andClosed; Described flash lamp frequency control module control flash lamp is opened flash of light, and controls flash time.In motion process, so circulation step S1-S6, thus complete the scanning to whole Tissue Culture Plate,Finally obtain the cellular morphology figure on whole Tissue Culture Plate.
The above the specific embodiment of the present invention, does not form limiting the scope of the present invention.Various other corresponding changes and distortion that any technical conceive according to the present invention has been done, all should wrapBe contained in the protection domain of the claims in the present invention.
Claims (11)
1. a control system for cell image acquisition device in cell cultivation, is characterized in that, described inControl system comprises data processing module, the first motion-control module, the second motion-control module, sudden strain of a muscleLight modulation frequency control module, image mechanism shutter control module and displacement detection module;
Described the first motion-control module, for according to the signal of described data processing module, controls and dodgesLight modulation block motion;
Described the second motion-control module, for according to the signal of described data processing module, control is taken the photographPicture mechanism kinematic;
Described image mechanism shutter control module, for according to the signal of described data processing module, controlsThe opening and closing of image mechanism shutter processed;
Described flash lamp frequency control module, for according to the signal of described data processing module, controlsThe flash time of flash lamp;
Described displacement detection module, for detection of the motion of described flash modules and described image mechanismDisplacement, and send displacement information to described data processing module;
Described data processing module, for sending respectively motor message to the first motion-control module andTwo motion-control modules, are synchronized with the movement described flash modules and described image mechanism; Also useIn described displacement information is processed, judge whether to open shutter and flash lamp; Also forSend shutter opening signal to described image mechanism, send flash lamp start signal to described flash lamp mouldPiece.
2. as the control system of cell image acquisition device in the cultivation of claim 1 cell, its feature existsIn, described flash modules is arranged on the first slide rail by slide block, and described image mechanism passes through slide blockBe arranged on the second slide rail, described flash modules and described image mechanism lay respectively at Tissue Culture PlateBoth sides up and down.
3. as the control system of cell image acquisition device in the cultivation of claim 1 cell, its feature existsIn, described cell image acquisition device also comprises motor, described the first motion-control module and describedDescribed in two moving control module for controlling, described in driven by motor, image mechanism and described flash modules are synchronously transportedMoving.
4. as the control system of cell image acquisition device in the cultivation of claim 1 cell, its feature existsIn, described data processing module is the machine platform controller during cell is cultivated.
5. as the control system of cell image acquisition device in the cultivation of claim 1 cell, its feature existsIn, described cell image acquisition device also comprises grating scale, described grating scale along described the first slide rail andDescribed the second slide rail be arranged in parallel, described displacement detection module detect described flash modules and described in take the photographThe distance that camera structure moves along described grating scale, and send a signal to described data processing module.
6. as the control system of cell image acquisition device in the cultivation of claim 1 cell, its feature existsIn, described control system is also for calculating according to the detected motor message of described displacement detection moduleAnd adjust the movement velocity of described flash modules and described image mechanism.
7. as the control system of cell image acquisition device in the cultivation of claim 1 cell, its feature existsIn, the shutter of described image mechanism is 10 milliseconds, the flash time of described flash modules is Microsecond gradeNot.
8. a control method for cell image acquisition device in cell cultivation, is characterized in that, comprisesFollowing steps:
S1, data processing module send respectively motor message to the first motion-control module and the second motionControl module, is synchronized with the movement flash modules and image mechanism;
S2, described the first motion-control module, according to the signal of described data processing module, are controlled flash of lightLamp module motion; Described the second motion-control module, according to the signal of described data processing module, is controlledThe motion of image mechanism;
S3, displacement detection module detect the moving displacement of described flash modules and described image mechanism,And send displacement information to described data processing module;
S4, described data processing module are processed displacement information, judge whether current motion distanceFrom the position that whether needs to open shutter and flash lamp, if so, perform step S5; If not, returnReturn step S3;
S5, described data processing module send shutter opening signal to described image mechanism, send flash of lightLamp start signal is to flash modules;
The opening and closing of S6, described image mechanism shutter control module controls image mechanism shutter; InstituteState flash lamp frequency control module control flash lamp and open flash of light, and control flash time.
9. as the control method of cell image acquisition device in the cultivation of claim 7 cell, its feature existsIt is the board in the cell culture system at cell image acquisition device place in, described data processing moduleController.
10. as the control method of cell image acquisition device in the cultivation of claim 7 cell, its featureBe, in described step S2, described the first motion-control module and described the second motion-control module controlDriven by motor image mechanism 3 processed and flash modules are synchronized with the movement.
11. as the control method of cell image acquisition device in the cultivation of claim 7 cell, its featureBe, in described step S3, described displacement detection module detects described flash modules and described shootingThe distance that mechanism moves along grating scale, and send a signal to described data processing module.
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Application publication date: 20160518 |