CN105585619B - With rice grain grain length and grain weight GAP-associated protein GAP and its encoding gene GL3-3 and application - Google Patents

Abstract

The invention discloses weigh GAP-associated protein GAP and its encoding gene and application with rice grain grain length and grain.The present invention provides a kind of albumen, are following protein 1) or 2): the 1) protein that the amino acid residue shown in sequence 3 in sequence table forms；2) by the amino acid residue sequence of protein shown in 1) by the substitution and/or deletion and/or addition of one or several amino acid residues and the protein with the same function as derived from 1).The experiment proves that, the present invention has cloned the gene GL3-3 of positive regulation rice grain length and grain weight, is conducted into OryzasativaLcv.Nipponbare, obtains transgenic paddy rice, the grain length and/or grain of transgenic paddy rice are great in OryzasativaLcv.Nipponbare rice, which provides new genetic resources for the high yield and high quality breeding of rice.

Description

With rice grain grain length and grain weight GAP-associated protein GAP and its encoding gene GL3-3 and application

Technical field

The present invention relates to field of plant genetic project technology, more particularly to rice grain grain length and grain weight GAP-associated protein GAP and Its encoding gene GL3-3 and application.

Background technique

One of grain weight and particle shape an important factor for being not only yield composition, have an effect on chalky grain rate, Coarse Rice Rate, polished rice rate, The quality traits such as head rice rate, thus influence rice appearance, mill, the qualities such as boiling and nutrition (Webb et al., 1991；Stone Spring sea etc., 1994；Xu Zhengjin etc., 2004；Meng Qinghong etc., 2009).Excavate rice grain weight and particle shape related gene and its clear function Can, only rice high yield and quality breeding do not provide excellent genetic resources, and facilitate clear rice grain weight and particle shape shape At molecule mechanism, have great theoretical and practical significance.

Grain again by grain length, grain is wide and the Grain shape traits such as grain is thick and kernel grouting codetermine, belong to quantitative inheritance.Mesh It is preceding it has been found that the site of control rice grain weight have nearly 286, wherein the grain of finely positioning weigh QTL have gw3.1 (Li et al., 2004), qGW8.1 (Xie et al., 2006), gw9.1 (Xie et al., 2008), qGL7 (Bai et al., 2010) and qGL7-2(Shao et al.,2010).Grain length and grain weight gene GS3 (Fan et al., 2006), GL3.1 (Qi et are cloned Al., 2012), grain is wide and grain weight gene GW2 (Song et al., 2007), qSW5 (Shomura et al., 2008；Weng et al.,2008；Wan et al., 2008) GS5 (Li et al., 2011) and GW8 (Wang et al., 2012).Except this it Outside, be still positioned to by near isogenic lines there are many QTL for influencing particle shape (Xing et al., 2002；Thomson et al.,2003；Li et al.,2004；Wang et al.,2012).In recent years, rice whole-genome association achieves Important breakthrough, navigated to multiple novel sites associated with Grain shape traits (Huang et al., 2010；Zhao et al., 2011；Huang et al.,2012).From the point of view of the above QTL distribution and quantity situation for positioning and cloning, grain length, grain weight are controlled Gene may be also very much.

Summary of the invention

It is an object of the present invention to provide weigh GAP-associated protein GAP and its encoding gene with rice grain grain length and grain.

Albumen provided by the invention, is named as GL3-3, is following protein 1) or 2):

1) protein that the amino acid residue shown in sequence 3 in sequence table forms；

2) by the amino acid residue sequence of protein shown in 1) by the substitution of one or several amino acid residues and/or Deletion and/or addition and the protein with the same function as derived from 1).

The substitution and/or deletion and/or addition of said one or several amino acid residues are that no more than 10 amino acid are residual The substitution and/or deletion and/or addition of base.

The DNA molecular for encoding above-mentioned albumen is also the scope of protection of the invention.

Above-mentioned DNA molecular is following 1) -5) in any DNA molecular:

1) code area is DNA molecular shown in sequence 2 in sequence table；

2) code area be in sequence table sequence 2 from the nucleotide of 5 ' end 10-1570；

3) code area be in sequence table sequence 2 from the nucleotide of 5 ' end 102-1106；

4) under strict conditions with 1) or 2) or 3) hybridize and encode the DNA molecular with identical function albumen；

5) with 1) or 2) or 3) DNA molecular with 90% or more homology and coding with identical function albumen.

Above-mentioned stringent condition can be for 0.1 × SSPE (or 0.1 × SSC), the solution of 0.1%SDS be miscellaneous in DNA or RNA It hands over and hybridizes at 65 DEG C in experiment and wash film.

Expression cassette, recombinant vector, recombinant bacterium, transgenic cell line or recombinant bacterium containing above-mentioned DNA molecular are also this hair The range of bright protection.

Above-mentioned recombinant vector is the recombinant vector for obtaining above-mentioned DNA molecular insertion expression vector, in implementation of the invention In example, expression vector pMDC32, recombinant vector is by sequence 2 from the nucleotides inserted of 5 ' end 10-1570 The carrier that (i.e. the downstream double tobacco mosaic virus promoter 35S) obtains between the site KpnI and PacI of pMDC32 carrier, is named as 35S::GL3-3。

Above-mentioned recombinant bacterium is that the recombinant vector is imported recombinant bacterium obtained in purpose bacterium, in the embodiment of the present invention In, purpose bacterium is Agrobacterium tumefaciems EHA105.

Above-mentioned albumen, above-mentioned DNA molecular, the expression cassette containing above-mentioned DNA molecular, recombinant vector, recombinant bacterium, transgenosis The application of cell line or recombinant bacterium in regulation plant products is also the scope of protection of the invention.

In above-mentioned application, the yield is embodied again by seed grain length and/or grain；

The regulation plant products are to improve plant seed grain length and/or grain weight；

The plant is specially monocotyledon or dicotyledon；The monocotyledon is specially rice.

It is a further object to provide a kind of methods for cultivating genetically modified plants.

Method provided by the invention obtains genetically modified plants for above-mentioned DNA molecular is imported purpose plant；

1) and/or 2) genetically modified plants have following feature:

1) the seed grain length of the genetically modified plants is greater than the purpose plant；

2) the seed grain of the genetically modified plants is great in the purpose plant；

The DNA molecular imports purpose plant by recombinant vector described in claim 4；

The plant is specially monocotyledon or dicotyledon；The monocotyledon is specially rice.

Above-mentioned albumen, above-mentioned DNA molecular, the expression cassette containing above-mentioned DNA molecular, recombinant vector, recombinant bacterium, transgenosis Cell line or recombinant bacterium are cultivating application and the scope of protection of the invention in high yield plant；The plant is specially unifacial leaf Plant or dicotyledon；The monocotyledon is specially rice.

The experiment proves that the present invention has cloned the gene GL3-3 of positive regulation rice grain length and grain weight, led Enter OryzasativaLcv.Nipponbare, obtains transgenic paddy rice, the grain length and/or grain of transgenic paddy rice are great in OryzasativaLcv.Nipponbare rice, which is rice High yield and high quality breeding provide new genetic resources.

The present invention is implemented as follows:

1, QTL scanning and positioning: using one by the especially big grain kind SLG-1 of rice (donor parents, hereinafter referred to as SLG) With backcrossing inbred strais (BILs) group of granule kind OryzasativaLcv.Nipponbare (Nipponbare, recurrent parent, hereinafter referred to as Nip) building Primary Location controls the QTL qGL3-3 of seed length and grain weight to one near the end mark RM1278 of No. three chromosome. Essence is carried out using the F2 group for about 2000 plants of near isogenic lines that village experiment station is planted in Beijing China Agricultural University in 2013 Target gene is located between label RM14484 and RM1278 in the section of about 280kb by fine positioning.

2, the determination of the association analysis of candidate region and candidate gene: utilize 272 parts of rice Mini core collection resource 14X's The SNP data (having 7 SNP in average 1kb) for the candidate region very high-density that high-precision sequencing obtains, the time that finely positioning is arrived Favored area is associated analysis.In the SNPs of highlights correlations, there are 19 integrated distributions in the starting of gene Os03g0183000 Subregion.Pass through the sequence comparing analysis to the gene region, it has been found that compared with OryzasativaLcv.Nipponbare, there are these SNP in SLG Therefore Os03g0183000 is determined as candidate gene (i.e. GL3-3) by point.The coded product of the gene have one it is conservative AP2 structural domain belongs to AP2/EREBP family.It is much sent out with plant growth it has been found that having in GL3-3 gene promoter region Educate relevant cis-acting elements.

3, the haplotype analysis of GL3-3: 5 most significant association sites according to GL3-3 promoter region (include 4 SNP and indel) Haplotyping A analysis is carried out to 247 parts of materials in the rice Mini core collection of this seminar collection, They can be divided into four kinds of haplotypes；According to Phylogenetic analysis, these four haplotypes be segmented into again Class A and Class B two major classes；Wherein, the seed length of Class A is considerably longer than Class B.

4, the gene functional verification of GL3-3: is found by Agrobacterium-mediated genetic transformation using overexpression technology The raising of expression quantity can make the grain length of transformation receptor rice " OryzasativaLcv.Nipponbare " rise to 7.81mm from 7.22mm；Mass of 1000 kernel from 21.14g increases to 23.25g, reaches extremely significant difference.

Detailed description of the invention

Fig. 1 is the molecular labeling schematic diagram of rice third chromosome mapping

SSR marker sequence is referring to http://www.gramene.org/.

Fig. 2 is the haplotype analysis result figure of GL3-3

Fig. 3 is the four kinds of haplotype cluster analysis result schematic diagrames obtained according to Phylogenetic analysis.

Fig. 4 is difference pair of obtained Class A and Class the B two major classes of four kinds of haplotype clusterings in seed length Than figure

Fig. 5 is expression vector pMDC32 map

Fig. 6 is that PCR identifies that T0 generation turns GL3-3 rice plant

Fig. 7 is the real-time fluorescence quantitative PCR testing result for turning OsGL3-3 rice plant

Fig. 8 is the phenotype comparison in difference figure for turning the grain length and grain weight and negative control plant of OsGL3-3 rice T2 generation

Fig. 9 is to turn OsGL3-3 rice T2 for the phenotype of seed in T2 generation

Specific embodiment

Experimental method used in following embodiments is conventional method unless otherwise specified.

The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.

Rice varieties SLG-1 is recorded in the following literature: the rice big grain germplasm resource such as Ma Lilian and genetic analysis plant Object notification, 2006,23 (4): 395-401；The public can obtain from China Agricultural University.

Rice varieties OryzasativaLcv.Nipponbare is recorded in the following literature: rice varieties " OryzasativaLcv.Nipponbare " agricultural science and technology communicates .1973 02 Phase；The public can obtain from China Agricultural University.

Carrier pMDC32 is recorded in the following literature: Eva M.Farre ' and Steve A.Kay.PRR7protein levels are regulated by light and the circadian clock in Arabidopsis.The Plant Journal,2007,52:548–560；The public can obtain from China Agricultural University, and the result schematic diagram of the carrier is figure 5。

Agrobacterium tumefaciems EHA105 is recorded in the following literature: the such as high generation force influence Agrobacterium tumefaciems EHA105 competence The torrid zone research biological journal .2012 the 1st phase of volume 3 March of cell transformation efficiency factor；The public can be from China Agricultural University It obtains.

The acquisition of embodiment 1, GL3-3 albumen and its encoding gene

1, the positioning of GL3-3 gene

Using one by the especially big grain kind SLG (donor parents) of rice and granule kind OryzasativaLcv.Nipponbare (Nipponbare, circulation Parent) building backcrossing inbred strais (BILs) group BC4F2 totally 175 strains, investigate each strain it is mature after mass of 1000 kernel, grain Length, grain is wide and grain is thick, the phenotypic data as QTL positioning.Meanwhile with BSA method from being evenly distributed on 12 chromosomes of rice On 1513 pairs of SSR markers in the polymorphism mark that filters out, marker bands are consistent with big grain parent SLG, A are denoted as, with Japan It is fine consistent, it is denoted as B, heterozygosis is denoted as H.Linkage mapping, Group command grouping, Kosambi is marked using Mapmaker3.0 Method calculates genetic distance.QTL scanning use QTL IciMapping 3.0 (Wang Jiankang etc., 2009), using LOD value 2.5 as Threshold value existing for QTL, while calculating additivity and dominant effect.The nomenclature principle of QTL is chain using naming methods such as McCouch Genetic map is completed in EXCEL 2010 using Liu et al. drawing practice.In the particle shape QTL detected, covers and much cloned Gene where region, such as grain length GS3 (Fan et al., 2006), the wide GW2 of grain (Song et al., 2007) and GW8 (Wang et al., 2012) etc..Meanwhile it scanning near the end mark RM1278 of No. three chromosome to a new control The QTL of seed length and grain weight, effect value reach 19%.In addition, all the QTL is arrived in scanning in multiple familys such as BC4F4.

Essence is carried out using about 2000 plants of NIL-F2 groups that village experiment station is planted in Beijing China Agricultural University in 2013 Target gene is located between common indicium RM14484 and RM1278 in the section of about 280kb (such as Fig. 1) by fine positioning.

2, the determination of the association analysis of candidate region and candidate gene

It is high close that candidate region is obtained by the high-precision sequencing to wherein 272 parts of rice Mini core collection resource 14X The SNP data (having 7 SNP in average 1kb) of degree, being associated analysis to the candidate region after finely positioning (has group structure Group's correction is carried out as covariant).In the SNP of highlights correlations, there are 19 integrated distributions gene Os03g0183000's Promoter region.By the comparison sequencing analysis discovery to the gene region, compared with OryzasativaLcv.Nipponbare, there are these SNP in SLG Point.The coding albumen (nucleotides sequence is classified as sequence 3) of the gene has a conservative AP2 structural domain, belongs to AP2/EREBP class Transcription factor family.AP2/EREBP class transcription factor member AP2 in arabidopsis is participated in establishing floral organ and floral meristem is special Sign, the uncertainty for inhibiting floral meristem and ovule and the development (Ohto et al.2005) for planting skin；Ohto etc. is additionally considered that Arabidopsis AP2 may be by influencing size of the glycometabolism to regulate and control seed.Still further, it was discovered that being opened in Os03g0183000 gene There are much cis-acting elements relevant to plant growth and development in sub-area.Therefore, which is determined as candidate gene GL3-3.The promoter region nucleotides sequence of GL3-3 gene is classified as sequence 1, and full-length cDNA nucleotides sequence is classified as sequence 2 in sequence table, The albumen of gene coding is named as GL3-3, and amino acid sequence is sequence 3 in sequence table.

3, the haplotype analysis of GL3-3

5 most significant association sites (including 4 SNP and 5 '-indel) according to GL3-3 promoter region are to this 247 parts of Mini core collections of rice that seminar collects carry out Haplotyping A analysis, as a result, it has been found that, they can be divided into four kinds Haplotype (Fig. 2)；With software MEGA5 carry out Phylogenetic analysis, these four haplotypes be segmented into again Class A with Class B two major classes；And the seed length of Class A is considerably longer than Class B (seeing figures 3 and 4).

The functional verification of embodiment 2, GL3-3 gene

One, turn the acquisition of GL3-3 rice

1, the acquisition of recombinant vector

The OryzasativaLcv.Nipponbare seedling cultivated under normal condition is taken, Trizol method extracts total serum IgE, uses M-MLV reverse transcriptase after purification It carries out reverse transcription and obtains cDNA.Using the cDNA as template, design primer F1 and primer R1 carry out PCR amplification, by amplified production into The DNA fragmentation of row agarose gel electrophoresis, recovery purifying 1580bp is sequenced.

As a result the PCR product has sequence 2 from the nucleotide of 5 ' end 10-1570, wherein from 5 ' ends the 102 to the 1106th open reading frame for GL3-3 gene.

The sequence of above-mentioned primer is as follows:

F1:5'-CGGGGTACCAAAGGCATTCGCAACACACA-3'(underscore base (4-9bp) is restriction enzyme The restriction endonuclease recognition sequence of enzyme KpnI)；

R1:5'-CCTTAATTAACCAAAATACATTACGACTGGAC-3'(underscore base (1571-1578bp) is limited The restriction endonuclease recognition sequence of property restriction endonuclease PacI processed).

By the PCR product KpnI and PacI double digestion of above-mentioned acquisition 1580bp, obtained digestion products with through by same The skeleton segment of the carrier pMDC32 of sample digestion is connected, and obtains recombinant vector.

By sequencing, which is by sequence 2 from the nucleotides inserted pMDC32 of 5 ' end 10-1570 The carrier that (i.e. the downstream double tobacco mosaic virus promoter 35S) obtains between the site KpnI and PacI of carrier, is named as 35S:: GL3-3。

2, the acquisition of recombinational agrobacterium

Above-mentioned recombinant vector 35S::GL3-3 freeze-thaw method is converted into Agrobacterium tumefaciems EHA105, acquisition contains recombinant vector The Agrobacterium tumefaciems EHA105 of 35S::GL3-3, i.e. recombinational agrobacterium EHA105/35S::GL3-3.

The plasmid of recombinational agrobacterium EHA105/35S::GL3-3 is extracted, KpnI and PacI digestion identification obtains 1580bp, For positive restructuring Agrobacterium.

3, the acquisition and identification of genetically modified plants

Rice OryzasativaLcv.Nipponbare (hereinafter also referred to wild rice) is infected with above-mentioned recombinational agrobacterium EHA105/35S::GL3-3 Embryo callus, obtaining 8 T0 generation turns GL3-3 rice strain, and the specific method is as follows:

1), the preparation of infected liquid

Recombinational agrobacterium EHA105/35S::GL3-3 is laid in kanamycins containing 50mg/L and 20mg/L rifampin In YEP culture medium, 28 DEG C are cultivated 2-3 days.Picking single bacterium spot be inoculated in YEP fluid nutrient medium (50mg/L kanamycins and 20mg/L rifampin), 28 DEG C, it is 0.8-1.0 that 240rpm, which is cultivated to OD600, is inoculated in YEP Liquid Culture by 1% inoculum concentration In base (50mg/L kanamycins and 20mg/L rifampin), 28 DEG C, it is 0.5-0.6 that 240rpm, which is cultivated to OD600, and bacterium is collected by centrifugation Weight is suspended from AAM culture medium 28 DEG C, and it is 0.3-0.4 as infected liquid that 240rpm, which is cultivated to OD600,.

2) it, infects and co-cultures

It chooses after the infected liquid that the embryo callus of good rice OryzasativaLcv.Nipponbare is prepared with step 1) is impregnated 30 minutes and takes Out, extra bacterium solution is sucked with aseptic filter paper, is subsequently placed in co-culture medium and cultivates 2-3 days.

3), screening and culturing

The callus that will be co-cultured through step 2) is with sterile water oscillation cleaning 3-4 times, then with 500mg/L cephalosporin water Solution oscillation washing 40 minutes, until supernatant cleans completely；Callus is taken out, the only sterile culture with filter paper is put into 0.4m/s is air-dried 4 hours in ware；Delay screening and culturing medium dark culture is transferred to be transferred in screening and culturing medium again after 3-7 days and screen two-wheeled (every wheel 3-4 weeks).

4), differentiation culture obtains transgenic plant

The callus that will be cultivated through step 3) is in pre- differential medium after dark culture 2-3 week, then moves on to differentiation and cultivate Illumination cultivation 2-3 weeks in base, when young shoot it is long to about 1cM when be transferred to strong seedling culture base culture 30 days, throw off sealed membrane hardening culture It one week, is then transplanted in soil, acquisition T0 generation turns GL3-3 rice.

Above-mentioned culture medium prescription is as shown in table 2.

Table 2 is culture medium prescription

Note: NB culture medium basis includes N6 a great number of elements, B5 microelement, B5 organic principle, 150mg/L inositol, 300mg/L caseinhydrolysate, 500mg/L glutamine, 600mg/L proline, 30g/L sucrose, 3g/L plant gel.

5) PCR is identified

GL3-3 rice plant seedling is turned to the T0 generation that step 4) obtains and extracts RNA, reverse transcription obtains cDNA as template, PCR expansion is carried out with primer 5 '-AAAAGTTCGACAGCGTCTCCGACC-3 ' and 5 '-TCTACACAGCCATCGGTCCAGACG-3 ' Increase.It is control with wild rice.

As a result as shown in fig. 6, p is plasmid 35S::GL3-3, W is wild rice, and 1-8 is to turn GL3-3 rice in T0 generation, can To find out, the target fragment for obtaining 919bp is to turn GL3-3 rice in positive T0 generation.

Being obtained 8 T0 generation turns GL3-3 rice strain.

Transgenosis present age plant is shown in T0 representative, and T1, which is represented, shows the T0 generation selfing seed generated and the plant grown up to by it, T2, which is represented, shows the T1 generation selfing seed generated and the plant grown up to by it, and T3 representative shows the seed that T2 generation selfing generates and by it The plant grown up to.

T0 generation turn GL3-3 rice strain OE4, OE5 seed sowing, cultivate, until obtain T2 turn GL3-3 rice strain OE4, OE5。

Using same method, empty carrier pMDC32 is transferred to wild rice, T0 is obtained for empty carrier rice is turned, trains Educate, obtain T2 generation turn empty carrier rice.

4, turn the real-time fluorescence quantitative PCR detection of GL3-3 rice

Wild type OryzasativaLcv.Nipponbare (WT), T2 are turned into GL3-3 rice strain OE4, OE5 in field planting, 10 plants of each strain.Point Each strain plant leaf is indescribably taken, total serum IgE is extracted using TRIZOL reagent and uses M-MLV reverse transcriptase using this RNA as template It carries out reverse transcription and obtains cDNA, using this cDNA as template, using primer 5 '-ATGGCTTGCTTGATTACCGAA-3 ' and 5 '- AGACCCCGTAAAAGTAGCCCA-3 ' is expanded, and the specific fragment (142bp) of GL3-3 gene is expanded.

It is control with Actin gene, the primer for expanding the gene is 5 '-ATTTGGCACCACACATTCTAC-3 ' and 5 '- ATAACCTTCGTAGATTGGGACT-3 ' amplifies the specific fragment (255bp) of rice Actin gene to carry out as internal reference Real-time quantitative analysis.

Real-time fluorescence quantitative PCR is in real-time fluorescence quantitative PCR instrument Applied Biosystems 7500Real Time It is carried out on PCR system (ABI, USA), a parallel test sets 3 repetitions.Utilize Livak KJ and Schmittgen TD (2001) method reported, i.e. 2- Δ Δ CT calculate relative expression quantity.

Δ Δ CT=(CT.Target-CT.Actin) Time x- (CT.Target-CT.Actin) Time 0

Time x indicates any point-in-time, and Time 0 indicates the target gene expression of 1 times of amount after actin is corrected.

As a result as shown in fig. 7, compared with wild rice, T2 turns GL3-3 gene in GL3-3 rice strain OE4, OE5 Expression quantity is apparently higher than wild rice.

Two, turn GL3-3 rice phenotype

It is laggard for empty carrier Rice Cropping crop field is turned that wild type OryzasativaLcv.Nipponbare (WT), T2 are turned into GL3-3 rice strain OE4 and T2 Row Phenotypic Observation, 20 plants of each strain, in triplicate, results are averaged for experiment.

Count each strain rice grain length and mass of 1000 kernel, as a result as shown in figure 8,

The grain length of wild type OryzasativaLcv.Nipponbare (WT) and mass of 1000 kernel are respectively 7.22mm and 21.14g；

T2 turns the grain length of GL3-3 rice strain OE4 (OE) and mass of 1000 kernel is respectively 7.81mm and 23.25g；

Observation T2 turns the seed phenotypes of GL3-3 rice strain OE4, as a result such as Fig. 9, in figure: above arranging grain is that T2 turns GL3-3 Rice strain, lower row's grain are the control of wild type OryzasativaLcv.Nipponbare, it can be seen that compared with wild type OryzasativaLcv.Nipponbare (WT), T2 turns GL3-3 The grain length of rice strain OE4 (OE) dramatically increases.

In wild type OryzasativaLcv.Nipponbare (WT) and T2 generation, turn empty carrier rice result without significant difference.

Thus, it will be seen that rice yield can be improved in GL3-3 gene, embodied by improving rice grain length and mass of 1000 kernel.

Claims (4)

1. the DNA molecular of albumen, encoding said proteins, the expression cassette containing the DNA molecular, recombinant vector, transgenic cell The application of system or recombinant bacterium in regulation plant seed grain length and/or grain weight；

The albumen is the protein that the amino acid residue shown in sequence 3 in sequence table forms；

The DNA molecular is following 1) -3) in any DNA molecular:

1) code area is DNA molecular shown in sequence 2 in sequence table；

2) code area be in sequence table sequence 2 from the nucleotide of 5 ' end 10-1570；

3) code area be in sequence table sequence 2 from the nucleotide of 5 ' end 102-1106；

The plant is specially monocotyledon；The monocotyledon is specially rice.

2. application according to claim 1, it is characterised in that:

The regulation plant seed grain length and/or grain weight are to improve plant seed grain length and/or grain weight.

3. a kind of method for cultivating genetically modified plants imports purpose plant for that will encode the DNA molecular of albumen, obtains transgenosis plant Object；

The albumen is the protein that the amino acid residue shown in sequence 3 in sequence table forms；

The DNA molecular is following 1) -3) in any DNA molecular:

1) code area is DNA molecular shown in sequence 2 in sequence table；

2) code area be in sequence table sequence 2 from the nucleotide of 5 ' end 10-1570；

3) code area be in sequence table sequence 2 from the nucleotide of 5 ' end 102-1106；

1) and/or 2) genetically modified plants have following feature:

1) the seed grain length of the genetically modified plants is greater than the purpose plant；

2) the seed grain of the genetically modified plants is great in the purpose plant；

The DNA molecular imports purpose plant by the recombinant vector in claim 1；

The plant is specially monocotyledon；The monocotyledon is specially rice.

4. the DNA molecular of albumen, encoding said proteins, the expression cassette containing the DNA molecular, recombinant vector, transgenic cell System or recombinant bacterium are cultivating the application in high yield plant；

The albumen is the protein that the amino acid residue shown in sequence 3 in sequence table forms；

The DNA molecular is following 1) -3) in any DNA molecular:

1) code area is DNA molecular shown in sequence 2 in sequence table；

2) code area be in sequence table sequence 2 from the nucleotide of 5 ' end 10-1570；

3) code area be in sequence table sequence 2 from the nucleotide of 5 ' end 102-1106；

The plant is specially monocotyledon；The monocotyledon is specially rice.