CN104561036B - Plant salt tolerance related gene PpSIG1 and its encoding proteins and application - Google Patents
Plant salt tolerance related gene PpSIG1 and its encoding proteins and application Download PDFInfo
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- CN104561036B CN104561036B CN201410770812.4A CN201410770812A CN104561036B CN 104561036 B CN104561036 B CN 104561036B CN 201410770812 A CN201410770812 A CN 201410770812A CN 104561036 B CN104561036 B CN 104561036B
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Abstract
The invention discloses a kind of plant salt tolerance related gene PpSIG1 and its application, its encoding amino acid sequence of plant salt tolerance related gene such as SEQ ID NO:Shown in 1, its nucleotide sequence of the gene of the albumen such as SEQ ID NO are encoded:Shown in 2, or its degenerate sequence.Channel genes rice of the present invention is overexpressed, it is possible to increase survival rate, growth rate and biomass under the salt stress of transgenic rice plant.Plant salt tolerance GAP-associated protein GAP PpSIG1 genes of the present invention provide the foundation to cultivate the crop that salt tolerance improves.
Description
Technical field
The invention belongs to biological technical field, is related to a kind of plant salt tolerance related gene PpSIG1 and its application.
Background technology
The soil salinization is a worldwide problem, according to UNESCO(UNESCO)And food and agricultural organization
(FAO)Incomplete statistics, about 1,000,000,000 hectares of the saline and alkaline land area in the whole world.At present, with being distributed in NORTHWEST CHINA, northeast and strand
The saline-alkali wasteland in area and saline and alkaline obstacle total cultivated area are more than 500,000,000 mu, wherein nearly 200,000,000 mu with agricultural use potentiality, account for me
More than the 10% of state total cultivated area.It is saline and alkaline that there is material impact for growth and development of plants, it is the important of limitation crop yield
Factor.Thus cultivate the important goal that salt tolerant crop new varieties are crop breedings.
An important means for improving plant salt endurance is that salt-resistant related gene is imported into plant by transgenic method, from
And improve the salt tolerance of transfer-gen plant.It is resistance to by genetic engineering means raising crop to excavate new salt-resistant related gene resource
The basis of salt.Halophytes obtains the resistance to environment salt stress in long-term evolutionary process, has in halophytes
Outstanding adversity gene resource.
The content of the invention
It is an object of the invention to provide one from be grown in China Fujian, Coast of Guangdong Province intertidal zone halophytes it is red
Tree Indian beech (Pongamia pinnata(L.) Pierre), the salt stress expression profile of salt tolerance of crop can be improvedPongamia pinnata Salt Induced Gene 1, abbreviation PpSIG1.
Plant salt tolerance related gene provided by the inventionPongamia pinnata Salt Induced Gene 1, referred to as
PpSIG1, from legume Indian beech (Pongamia pinnata(L.) Pierre), its encoding proteins amino acid sequence
Row such as SEQ ID NO:Shown in 1.
SEQ ID NO:1 sequence is made up of 362 amino acid residues.
The present invention is also provided SEQ ID NO:1 amino acid sequence passes through the substitution of one or several amino acid residues
And/or missing and/or addition and it is related to plant stress tolerance by SEQ ID NO:Protein derived from 1 sequence.
In order that described plant salt tolerance GAP-associated protein GAP PpSIG1 is easy to purify, can be by SEQ ID NO:Ammonia shown in 1
The amino terminal or the upper label as shown in table 1 of carboxyl terminal connection of the protein of base acid sequence composition.
The sequence of the label of table 1
It is above-mentioned by SEQ ID NO:Protein can be artificial synthesized derived from 1 sequence, also can first synthesize its encoding gene, then enter
Row biological expression obtains, and its encoding gene can be by by SEQ ID NO:One or several amino are lacked in DNA sequence dna shown in 2
The codon of sour residue, and/or the missense mutation of one or several base-pairs is carried out, and/or connected at its 5 ' end and/or 3 ' ends
The coded sequence of label shown in table 1 obtains.
The present invention also provides a kind of coding the plant salt tolerance related gene PpSIG1, its nucleotide sequence such as SEQ ID
NO:Shown in 2, or its degenerate sequence.
Meanwhile the present invention also provides such as SEQ ID NO:DNA sequence dna shown in 2 has more than 90% homology, and encodes resistance to
The DNA molecular of inverse property GAP-associated protein GAP.
The present invention be additionally provided under strict conditions with SEQ ID NO:DNA sequence dna shown in 2 hybridizes and encodes the egg
White DNA molecular;Also provide simultaneously has more than 90% homology with the DNA molecular, and encodes the DNA of stress tolerance correlative protein
Molecule;The stringent condition can be in 6 × SSC, 0.5% SDS solution, 65oHybridize under C, then with 2 × SSC, 0.1%
SDS and 1 × SSC, 0.1% SDS respectively wash film once.
The present invention also provides the recombinant expression carrier of the gene.
The recombinant expression carrier of the gene can be contained with existing plant expression vector construction.
The plant expression vector includes double base agrobacterium vector and the carrier available for plant micropellet bombardment etc..The plant
Thing expression vector can also include 3 ' end untranslated regions of foreign gene, i.e., comprising polyadenylation signals and any other participation
MRNA is processed or the DNA fragmentation of gene expression.The bootable polyadenylic acid of polyadenylation signals is added to the 3 ' of mRNA precursor
End, such as Agrobacterium crown gall nodule induction (Ti) plasmid gene (such as kermes synzyme Nos genes), plant gene(Such as soybean storage egg
White gene)The non-translational region of 3 ' end transcriptions is respectively provided with similar functions.
During using the gene constructed recombinant plant expression vector, any one can be added before its transcription initiation nucleotides
Enhanced promoter or constitutive promoter, such as cauliflower mosaic virus(CAMV)The ubiquitin promoter of 35S promoter, corn
(Ubiquitin), they can be used alone or are used in combination with other plant promoters;In addition, the gene using the present invention
When building plant expression vector, enhancer, including translational enhancer or transcriptional enhancer also can be used, these enhancer regions can
To be ATG initiation codon or neighboring region initiation codon etc., but must be identical with the reading frame of coded sequence, it is whole to ensure
The correct translation of individual sequence.The source of the translation control signal and initiation codon is extensive, can be natural, also may be used
Be synthesis.Translation initiation region can come from transcription initiation region or structural gene.
For the ease of transgenic plant cells or plant are identified and screened, plant expression vector used can be carried out
Processing, the enzyme of color change or the gene of luminophor can be produced as added the coding that can be expressed in plant(Gus gene,
Luciferase genes etc.), resistant antibiotic marker(Gentamicin label, kanamycins label etc.)It is or anti-
Chemical reagent marker gene(Such as anti-herbicide gene)Deng.From the security consideration of genetically modified plants, any selectivity can be not added with
Marker gene, transformed plant is directly screened with adverse circumstance.
The recombinant expression carrier is the recombinant plasmid for obtaining gene insertion pCXUN multiple cloning sites.
Contain gene described in any of the above(PpSIG1)Expression cassette, transgenic cell line and recombinant bacterium belong to the present invention
Protection domain.
Expand the gene(PpSIG1)The primer pair of total length or any fragment falls within protection scope of the present invention.
The present invention also provides the gene(PpSIG1)Salt resistance ability enhancing genetically modified plants in application, its be by
Recombinant expression carrier containing the gene imports the genetically modified plants that purpose plant obtains salt resistance ability enhancing.
Using any carrier that foreign gene can be guided to be expressed in plant, the gene of encoding said proteins is led
Enter plant cell, the transgenic cell line and transfer-gen plant of salt resistance ability enhancing can be obtained.Carry the expression of the gene
Carrier can be by using Ti-plasmids, Ri plasmids, plant viral vector, directly delivered DNA, microinjection, conductance, agriculture bacillus mediated
Plant cell or tissue are converted Deng conventional biology methods, and the plant tissue of conversion is cultivated into plant.The plant being converted
Host both can be monocotyledon or dicotyledon, such as:Tobacco, crowtoe, arabidopsis, rice, wheat, jade
Rice, cucumber, tomato, willow, turfgrass, lucerne place etc..
Experiment shows, by the encoding regulator plant salt tolerance related gene of the present inventionPpSIG1 DNA sequence dna Introduced into Rice in
It is overexpressed, it is possible to increase transgenic paddy rice salt tolerance, improve survival rate, growth rate under the salt stress of transgenic rice plant
And biomass.The coded plant salt-resistant related gene of the present inventionPpSIG1Gene has the crop of salt tolerance raising to cultivate other
Provide the foundation.
Brief description of the drawings
Fig. 1 identifies to be overexpressed transfer-gen plant PCR.
Fig. 2 identifies to be overexpressed transfer-gen plant real-time quantitative PCR.
Fig. 3 is that water planting control and salt stress handle wild type and PpSIG1 transgenic lines ox1.
Fig. 4 is that water planting control and salt stress handle wild type and PpSIG1 transgenic lines ox1 and ox2 survival rate.
Fig. 5 is that water planting control and salt stress handle wild type and PpSIG1 transgenic lines ox1 and ox2 plant height.
Fig. 6 is that earth culture experiment contrast and salt stress handle wild type and PpSIG1 transgenic lines ox1 and ox2.
Fig. 7 is that earth culture experiment contrast and salt stress handle wild type and PpSIG1 transgenic lines ox1 and ox2 survival
Rate.
Fig. 8 is that earth culture experiment contrast and salt stress handle wild type and PpSIG1 transgenic lines ox1 and ox2 plant height.
Fig. 9 is that earth culture experiment contrast and salt stress handle wild type and PpSIG1 transgenic lines ox1 and ox2 overground part
Divide single-strain fresh weight.
Embodiment
Below by embodiment detailed description come the present invention is furture elucidated, but be not to the present invention limit
System, is only illustrated.
Experimental method in following embodiments, it is conventional method unless otherwise specified, test material used, such as nothing
Specified otherwise, it is to be commercially available from routine biochemistry reagent shop, experiment is respectively provided with to be repeated three times, results averaged.
Embodiment 1:PpSIG1 cDNA clone
Because currently without Indian beech Genomic sequence information, we utilize Illumina Genome Analyzer
IIx carries out high-flux sequence to salt stress processing and untreated control Indian beech transcript profile, and the original series obtained filter off
After joint and low quality sequence, using SOAPdenovo programs (http://soap.genomics.org.cn) carry out sequence
Assembling, non repetitive sequence (Unigenes) is obtained, BLASTx program search Nr, Swiss-Prot, KEGG are used to Unigenes
With COG databases, using annotation of the similitude highest Sequence annotation as the Unigene.Each base is calculated by RPKM values
The expression of cause, we, which screen, obtains 1050 genes induced by salt stress.These genes overwhelming majority does not enter at present
Row functional analysis, the new gene resource of key function is played to excavate Indian beech in salt stress response, from unknown function
In gene, we select rapid elevated 10 genes of induced expression in salt stress processing, are cloned and pass through transgenosis
Function in method validation its transgenic paddy rice salt stress reaction.Functional analysis confirms that one of them is yellow by the water of salt stress induction
Skin Unigene can improve the salt tolerance of transgenic paddy rice after being overexpressed in rice, we are named as PpSIG1ORF.Experiment
Method is as follows, according to the following special primer of deduction sequences Design:
Sense primer:5’-ATGGAAGAGATCAAGAAACC-3’;
Anti-sense primer:5’-TCAGCTTCGATTGTGATGTTC-3’.
With 200 mM NaCl handle a monthly age Indian beech (Pongamia pinnata(L.) Pierre) plant 6 hours
Afterwards, 0.2g Indian beech blades, liquid nitrogen grinding, TRIzol methods extraction total serum IgE are taken.Take 2 μ g total serum IgE SuperScript II
RT reverse transcriptase carries out reverse transcription, synthesizes the chains of cDNA first, as template, enters performing PCR reaction with above-mentioned special primer.PCR
Product is cloned into pEASY-T after electrophoretic separation reclaims(Beijing Quanshijin Biotechnology Co., Ltd), it is named as pEASY-T-
PpSIG1ORF, it is sequenced.
Sequencing result shows, the nucleotide sequence such as SEQ ID NO of the fragment:Shown in 2, SEQ ID NO are encoded:Shown in 1
Protein.
Embodiment 2:Overexpressions of the PpSIG1 in rice
First, the structure of pCXUN-PpSIG1 expression vectors
1st, using recombinant plasmid pEASY-T-PpSIG1ORF in implementing 1 as masterplate, using HiTaq archaeal dna polymerases and following
Row primer amplified obtains PpSIG1 sequences.
Sense primer:5’-ATGGAAGAGATCAAGAAACC-3’;
Anti-sense primer:5’-TCAGCTTCGATTGTGATGTTC-3’.
After PCR primer electrophoretic separation, the bp DNA fragmentations of gel extraction 1089.
2nd, with restriction enzyme XcmI digestions pCXUN (Ubiquitin promoters) (Plant Physiol. 2009
July; 150(3):1111-1121) skeleton, is reclaimed.
3rd, the fragment that step 1 obtains is connected with the fragment that step 2 obtains, converts bacillus coli DH 5 alpha, sequencing confirms just
Really, pCXUN-PpSIG1 is obtained.
2nd, the acquisition of genetically modified plants
1st, recombinant expression carrier pCXUN-PpSIG1 is imported into Agrobacterium AGL0 (ATCC BAA-100 using electric shocking method
, www.atcc.org).
5th, the Agrobacterium AGL0 containing pCXUN-PpSIG1 is infected into embryonic type callus group caused by the induction of Nipponbare wild type
Knit, then the screening resistant calli in MS culture mediums (containing 30mg/L hygromycin), per 15 days generations, altogether 3 generation, then
Resistant calli is induced into intact plant, rice transplanting and harvests the T1 of genetically modified plants for seed in crop field.
3rd, the Molecular Detection of genetically modified plants
CTAB methods extract leaf DNA, with-the AAGAGTTGCTCGTCACTG-3 ' of pCXUN-PpSIG1 specific primers 5 ' and
5 '-TCGGGTTGTGGAATGTTG-3 ' PCR are detected, the results showed that there is specific amplification band in transfer-gen plant, and wild type
For DNA without specific amplified band (Fig. 1), this shows that PpSIG1 has been introduced into transfer-gen plant.
TRIzol methods extract 2 week old wild types and PpSIG1 transfer-gen plants (ox1,2) blade total serum IgE, take 2 μ g total
RNA, with 2 u RQ1 RNase-free DNase (Promega), 37 °C handle 30 min, after adding terminating reaction liquid, with
PolyA is primer, and the chains of cDNA first are synthesized within 1 hour using M-MLV reverse transcriptase 42 °C, then using cDNA as template, with
Ubiquitin (primer 5 '-CCATCCTCAAGCTGCTTACC-3 ' and 5 '-GACTGGCAAGACCATTACCC-3 ') is internal reference,
PCR method detection PpSIG1 genes (- the TATTTCCACAGCTCCCTCTTC-3 ' and 5 ' of primer 5 '-
CCCACCACAACATCTTGTTTC-3 ') expression, as a result as shown in Figure 2.PpSIG1 is can't detect in Wild type control plants
The expression of gene, and pCXUN-PpSIG1 transfer-gen plants (ox1,2) PpSIG1 gene expression doses are about Ubiquitin tables
Up to horizontal 0.8-0.9 times.
Embodiment 3:PpSIG1 transgenic rice plants salt tolerance detects
First, water planting rice plant salt tolerance detects
After wild type and the processing 3 days of 42 °C of PpSIG1 transgenic paddy rice seeds, soaked seed 24 hours in water, 37 °C of vernalization,
The consistent seed of rudiment is chosen in following nutrient solutions, 28 °C are cultivated 4 days, are then located in the nutrient solution containing 100 mM NaCl
Reason 4 days.As a result as in Figure 3-5, wild type and PpSIG1 transgenic rice plants are not significantly different in untreated control,
After 100 mM NaCl processing, WT lines survival rate is 35%, the cm of plant height 13.2, substantially less than PpSIG1 transgenosis
Survival rate 60-65% and plant height, 19.2-19.5 cm.This show water planting salt stress processing in, PpSIG1 transgenic rice plants
Salt tolerance is significantly higher than wild type control.
Cultivate formula of liquid:The mg/L of four water-calcium nitrate 945, the mg/L of potassium nitrate 506, the mg/L of ammonium nitrate 80, phosphoric acid
The mg/L of potassium dihydrogen 136, magnesium sulfate 493 mg/L, 2.5 ml of iron salt solutions/L, micro- mother liquor 5 ml/L, pH=
6.0。
Iron salt solutions:Ferrous sulfate heptahydrate 2.78g, disodium ethylene diamine tetraacetate(EDTA.Na)3.73g distilled water
500ml, pH=5.5.
Micro- mother liquor:The mg/l of KI 0.83, boric acid 6.2 mg/L, manganese sulfate 22.3mg/L, sulfuric acid
Zinc 8.6mg/L, sodium molybdate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L.
2nd, earth culture rice plant salt tolerance detects
After wild type and the processing 3 days of 42 °C of PpSIG1 transgenic paddy rice seeds, soaked seed 24 hours in water, 37 °C of vernalization,
The consistent seed kind of rudiment is chosen in the small basin equipped with Nutrition Soil, covers one layer of preservative film moisturizing, after coming up, is removed
Preservative film, after 2 week, the small basin of rice cultivation seedling is put into the water containing 100 mM NaCl and soaked 1 week, then pours out salt solution,
A running water is changed daily, is taken a picture after 1 week, counts physiological phenotype.As Figure 6-9, in the processing of no salt stress to according to the facts
In testing, PpSIG1 transgenic paddy rice strains ox1 and ox2 grow no notable difference, and 1 week is handled then by 100 mM NaCl
Rehydration, the survival rate of Wild type control plants only has 24%, and PpSIG1 transgenic paddy rice strains ox1 and ox2 survival rate are distinguished
For 39% and 45%, it is significantly higher than wild type control.It is similar as shown in FIG. 8 and 9, PpSIG1 transgenic paddy rice strains ox1 and ox2
Two physical signs of plant height and fresh weight be also significantly greater than Wild type control plants.Result above shows, is tested in earth culture
In, the salt tolerance of PpSIG1 transgenic paddy rice strains significantly improves.
<110>Biological Technology institute, Chinese Academy of Agricultural Sciences
<120>Plant salt tolerance related gene PpSIG1 and its encoding proteins and application
<160> 2
<210> 1
<211> 362
<212> PRT
<213> Pongamia pinnata
<400> 1
Met Glu Glu Ile Lys Lys Pro Phe Gly Thr Ser Leu Leu Val Pro Ser
Val Gln Glu Leu Ala Glu Gly Lys Ile Ser Asn Val Pro Asp Arg Tyr
Ile Gln Pro Gln Gln His Glu Glu Leu Leu Val Thr Glu Ala Asp Tyr
His Val Leu Glu Ile Pro Val Ile Asp Met Gln Asn Leu Leu Ser Leu
Glu Ser Gly Ala Ser Glu Leu Thr Lys Leu His Leu Ala Cys Arg Tyr
Trp Gly Phe Phe Gln Leu Val Asn His Gly Val Ser Ser Leu Leu Leu
Glu Lys Val Lys Leu Glu Ile Gln Asn Phe Phe Asn Leu Pro Met Leu
Glu Lys Lys Lys Phe Trp Gln Ser Pro Gln His Met Glu Gly Phe Gly
Gln Gly Phe Val Val Ser Glu Asp Gln Lys Leu Asp Trp Ala Asp Met
Phe Tyr Met Thr Thr Leu Pro Thr Lys Gln Arg Ile Pro His Leu Phe
Pro Gln Leu Pro Leu Pro Phe Arg Asp Thr Met Glu Leu Tyr Ser Gln
Asp Val Lys Asn Ile Ala Leu Ile Ile Ile Ala His Ile Glu Lys Ala
Leu Lys Met Glu Glu Met Glu Ile Met Lys Leu Phe Glu Asp Leu Arg
Gln Thr Met Arg Ile Asn Tyr Tyr Pro Pro Cys Pro Glu Pro Glu Lys
Val Ile Gly Leu Thr Pro His Ser Asp Gly Thr Gly Leu Thr Ile Leu
Leu Gln Val Asn Glu Val Glu Gly Leu Gln Ile Lys Lys Asp Gly Met
Trp Val Pro Ile Met Pro Leu Pro Asn Ala Phe Ile Val Asn Ile Gly
Glu Ile Leu Glu Ile Ile Thr Asn Gly Ile Tyr Gln Ser Ile Glu His
Arg Ala Thr Val Asn Ser Glu Lys Glu Arg Leu Ser Ile Ala Thr Phe
His Asn Pro Lys Gln Asp Val Val Val Gly Pro Ala Ala Ser Leu Ile
Thr Glu Gln Ile Pro Ala Gln Phe Lys Arg Ile Arg Ile Glu Glu Tyr
Leu Arg Gly Ile Phe Ala Arg Lys Leu Asn Gly Lys Ser Tyr Leu Asp
Thr Phe Gly Ile Glu His His Asn Arg Ser
<210> 2
<211> 1089
<212> DNA
<213> Pongamia pinnata
<400> 2
atggaagaga tcaagaaacc atttgggact tctcttctgg tgccatcagt tcaagaattg 60
gctgaaggga aaatatcaaa tgttccagac agatacattc agcctcaaca acatgaagag 120
ttgctcgtca ctgaagctga ttatcatgtc cttgagattc cagttattga catgcagaac 180
ttgctttctc tagaatctgg tgcttctgag ttgaccaagc ttcaccttgc ttgcagatat 240
tggggattct tccagctggt aaaccatgga gttagttctt tattgctgga aaaggtaaag 300
ttggagattc agaatttttt caaccttcca atgctggaaa agaaaaaatt ttggcagagt 360
ccgcaacata tggagggatt tggacaagga tttgttgtta gtgaagacca aaaacttgat 420
tgggctgaca tgttctatat gacaaccctt ccaacaaagc agaggatccc ccacttattt 480
ccacagctcc ctcttccgtt cagggacact atggagcttt actcacaaga cgtgaaaaat 540
atagccttga ttattattgc acacattgag aaagctctta agatggagga aatggaaata 600
atgaagttat ttgaagactt gagacagact atgaggataa actattaccc tccatgtcca 660
gaaccggaga aggttattgg ccttactccc cattcagatg gaactggtct cactatcctt 720
cttcaagtta atgaggtgga agggctccag ataaagaaag atggaatgtg ggttcctatt 780
atgcccctgc ctaatgcatt cattgttaac attggcgaga tacttgagat tataaccaat 840
ggtatatacc aaagtattga acacagagca acagtgaact ctgaaaaaga aaggctttca 900
attgcaacat tccacaaccc gaaacaagat gttgtggtgg gtcctgcggc tagcttaatc 960
actgagcaaa taccagcaca gtttaaaaga ataagaattg aagaatattt gaggggcata 1020
tttgctcgta aacttaacgg aaagtcttac ctagatacct ttggaataga acatcacaat 1080
cgaagctga 1089
Claims (10)
- A kind of 1. plant salt tolerance related gene PpSIG1, it is characterised in that:Its protein amino acid sequence such as SEQ ID encoded NO:Shown in 1.
- 2. gene as claimed in claim 1, it is characterised in that:Its protein amino acid sequence such as SEQ ID NO encoded:1 institute Show, further comprise that coding is connected with sequence label in its amino terminal or carboxyl terminal.
- 3. gene as claimed in claim 2, it is characterised in that:Described sequence label be Poly-Arg, Poly-His, FLAG, Strep-tag II or c-myc.
- 4. the expression cassette containing the gene as described in any one of claims 1 to 3, it is characterised in that the gene can with promoter Operation connection.
- 5. the recombinant expression carrier containing the gene as described in any one of claims 1 to 3.
- 6. recombinant expression carrier as claimed in claim 5, it is characterised in that:It is double base agrobacterium vector.
- 7. recombinant expression carrier as claimed in claim 6, it is characterised in that:The carrier is the load for plant micropellet bombardment Body.
- 8. recombinant expression carrier as claimed in claim 5, it is characterised in that:The recombinant expression carrier is to insert the gene Enter the recombinant plasmid that pCXUN multiple cloning sites obtain.
- 9. the recombinant host cell containing the gene as described in any one of claims 1 to 3.
- 10. application of the gene in the genetically modified plants that salt resistance ability strengthens as described in any one of claims 1 to 3, it is Recombinant expression carrier containing the gene is imported to the genetically modified plants of purpose plant acquisition salt resistance ability enhancing, the plant It is rice.
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CN103468710A (en) * | 2013-08-20 | 2013-12-25 | 深圳大学 | Pongamia pinnata stress tolerance relative gene MpSRG as well as coded protein and application thereof |
CN103468711A (en) * | 2013-08-20 | 2013-12-25 | 深圳大学 | Pongamia pinnata stress tolerance relative gene MpZFP as well as coded protein and application thereof |
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CN103468711A (en) * | 2013-08-20 | 2013-12-25 | 深圳大学 | Pongamia pinnata stress tolerance relative gene MpZFP as well as coded protein and application thereof |
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Sequence and expression divergence of the AOC gene family in soybean: insights into functional diversity for stress responses;QIAN WU.et al;《Biotechnol Lett》;20110406;1351-1359 * |
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