CN105585619A - Protein related to grain length and grain weight of paddy rice grains, encoding gene GL3-3 and application thereof - Google Patents

Abstract

The invention discloses a protein related to grain length and grain weight of paddy rice grains, and an encoding gene and an application thereof. The protein can be: 1) a protein which is composed of the amino acid residues represented as the sequence No.3 in a sequence table; and 2) a protein, which is derived by substituting and/or deleting and/or adding one or more amino acid residues to the amino acid residues of the protein 1), wherein the protein 2) has the same functions of the protein 1). A test proves that the gene GL3-3 which positively regulates the grain length and grain weight of paddy rice is cloned and then is introduced into Nipponbare to obtain transgenic rice, of which the grain length and the grain weight are higher than those of the Nipponbare. The gene provides a new genetic source for high yield and high quality breeding of the paddy rice.

Description

With rice grain grain length and grain re-correlation albumen and encoding gene GL3-3 and application

Technical field

The present invention relates to plant gene engineering technology field, relate in particular to rice grain grain length and grain re-correlation albumen andIts encoding gene GL3-3 and application.

Background technology

Heavy and the particle shape of grain is not only one of key factor that output forms, also affect the white rate of chalk, brown rice rate, polished rice rate,The quality traits such as head rice rate, thus affect rice outward appearance, mill, quality (Webbetal., 1991 such as boiling and nutrition;Shi Chunhai etc., 1994; Xu Zhengjin etc., 2004; Meng Qinghong etc., 2009). Excavate rice grain weight and particle shape related geneAnd clear and definite its function, not only for rice high yield and quality breeding provide excellent genetic resources, and contribute to clear and definite waterThe molecule mechanism that the grain of rice is heavy and particle shape forms, has important theory and practice meaning.

Grain is determined by the wide and grain particle shape proterties such as thick of grain length, grain and kernel grouting heavily jointly, all belongs to quantitative inheritance.The site of the control rice grain weight of having found at present has nearly 286, and wherein the heavy QTL of the grain of meticulous location has gw3.1 (Lietal.,2004)，qGW8.1(Xieetal.,2006)，gw9.1(Xieetal.,2008)，qGL7(BaiEtal., 2010) and qGL7-2 (Shaoetal., 2010). Grain length and the heavy gene GS3 of grain (Fanet are clonedAl., 2006), the wide and heavy gene GW2 of grain (Songetal., 2007) of GL3.1 (Qietal., 2012), grain,qSW5(Shomuraetal.,2008；Wengetal.,2008；Wanetal.,2008)GS5(Lietal.,2011) and GW8 (Wangetal., 2012). In addition, still there are many QTL that affect particle shape by near etc.Gene line is positioned to (Xingetal., 2002; Thomsonetal., 2003; Lietal., 2004; WangEtal., 2012). In the last few years, paddy rice whole-genome association had been obtained important breakthrough, navigated to multiple withNovel site (Huangetal., 2010 that particle shape proterties is associated; Zhaoetal., 2011; Huangetal.,2012). Distribute and quantity situation from above location and clone's QTL, control grain length, the gene that grain is heavy may be gone backA lot.

Summary of the invention

An object of the present invention is to provide and rice grain grain length and grain re-correlation albumen and encoding gene thereof.

Albumen provided by the invention, called after GL3-3, is following 1) or 2) protein:

1) protein being formed by the amino acid residue shown in sequence in sequence table 3;

2) by 1) shown in protein amino acid residue sequence through the replacement of one or several amino acid residue and/Or disappearance and/or add and have identical function by 1) derivative protein.

The replacement of above-mentioned one or several amino acid residue and/or disappearance and/or be added to that to be no more than 10 amino acid residualThe replacement of base and/or disappearance and/or interpolation.

The DNA molecular of above-mentioned albumen of encoding is also the scope of protection of the invention.

Above-mentioned DNA molecular is following 1)-5) in arbitrary described DNA molecular:

1) code area is the DNA molecular shown in sequence 2 in sequence table;

2) code area be in sequence table sequence 2 from 5 ' end 10-1570 position nucleotides;

3) code area be in sequence table sequence 2 from 5 ' end 102-1106 position nucleotides;

4) under stringent condition with 1) or 2) or 3) hybridization and coding have the DNA molecular of identical function albumen;

5) with 1) or 2) or 3) 90% above homology and coding there is and have the DNA molecular of identical function albumen.

Above-mentioned stringent condition can be with 0.1 × SSPE (or 0.1 × SSC), the solution of 0.1%SDS, at DNA orIn RNA hybrid experiment, at 65 DEG C, hybridize and wash film.

Expression cassette, recombinant vector, recombinant bacterium, transgenic cell line or the recombinant bacterium that contains above-mentioned DNA molecular is also thisThe scope of invention protection.

Above-mentioned recombinant vector is that above-mentioned DNA molecular is inserted to the recombinant vector that expression vector obtains, in enforcement of the present inventionIn example, expression vector is pMDC32, and recombinant vector is from 5 ' end 10-1570 position nucleosides by sequence table sequence 2Between the KpnI and PacI site of acid insertion pMDC32 carrier, (i.e. two tobacco mosaic disease virus promoter 35S downstream) obtainsCarrier, called after 35S::GL3-3.

Above-mentioned recombinant bacterium is that described recombinant vector is imported to the recombinant bacterium obtaining in object bacterium, at embodiments of the inventionIn, object bacterium is Agrobacterium tumefaciems EHA105.

Above-mentioned albumen, above-mentioned DNA molecular, the expression cassette that contains above-mentioned DNA molecular, recombinant vector, recombinant bacterium,The application in regulating plant output of transgenic cell line or recombinant bacterium is also the scope of protection of the invention.

In above-mentioned application, described output heavily embodies by seed grain length and/or grain;

Described regulating plant output is that raising plant seed grain length and/or grain are heavy;

Described plant is specially monocotyledon or dicotyledon; Described monocotyledon is specially paddy rice.

Another object of the present invention is to provide a kind of method of cultivating genetically modified plants.

Method provided by the invention, for above-mentioned DNA molecular is imported to object plant, obtains genetically modified plants;

Described genetically modified plants have following 1) and/or 2) feature:

1) the seed grain length of described genetically modified plants is greater than described object plant;

2) the seed grain of described genetically modified plants is great in described object plant;

Described DNA molecular imports object plant by recombinant vector described in claim 4;

Described plant is specially monocotyledon or dicotyledon; Described monocotyledon is specially paddy rice.

Above-mentioned albumen, above-mentioned DNA molecular, the expression cassette that contains above-mentioned DNA molecular, recombinant vector, recombinant bacterium,Transgenic cell line or recombinant bacterium are also the scope of protection of the invention in the application of cultivating in high yield plant; Described plant toolBody is monocotyledon or dicotyledon; Described monocotyledon is specially paddy rice.

The present invention of experiment showed, of the present invention has cloned positive regulation paddy rice grain length and the heavy gene GL3-3 of grain, by itImport Japan fine, obtain transgenic paddy rice, the grain length of transgenic paddy rice and/or grain are great in the fine paddy rice of Japan, this geneFor the high yield and high quality breeding of paddy rice provides new genetic resources.

The present invention is achieved in that

1, QTL scanner uni location: (donor parents is designated hereinafter simply as by the especially big grain of paddy rice kind SLG-1 to utilizeSLG) and granule kind Japan fine (Nipponbare, recurrent parent are designated hereinafter simply as Nip) build the inbreeding that backcrossesNear (BILs) colony of system Primary Location to No. three chromosomal end mark RM1278 control seed length andThe QTLqGL3-3 that grain is heavy. Utilize approximately 2000 strains of village experiment station plantation in Beijing China Agricultural University in 2013 nearThe F2 colony of isogenic line carries out meticulous location, and target gene is positioned between mark RM14484 and RM1278 approximatelyIn the interval of 280kb.

2, determining of the association analysis of candidate region and candidate gene: utilize 272 parts of paddy rice Mini core collection resource 14XThe SNP data (having 7 SNP in average 1kb) of the candidate region very high-density that obtains of high accuracy order-checking, to meticulousAssociation analysis is carried out in the candidate region navigating to. In highly associated SNPs, there are 19 concentrated genes that are distributed inThe promoter region of Os03g0183000. By the sequence comparing analysis to this gene region, we find with Japan fineCompare, in SLG, have these SNP sites, therefore, Os03g0183000 is defined as to candidate gene (being GL3-3).The coded product of this gene has a conservative AP2 domain, belongs to AP2/EREBP family. We also findGL3-3 gene promoter region has a lot of cis-acting elements relevant to growth and development of plants.

3, the haplotype analysis of GL3-3: 5 associated sites the most significant according to GL3-3 promoter region (comprise4 SNP and an indel) 247 parts of materials in the paddy rice Mini core collection of this seminar collection are carried out to haplotypePhenotypic analysis, they can be divided into four kinds of haplotypes; Analyze according to system, these four kinds of haplotypes can divide againBecome ClassA and the large class of ClassB two; Wherein, the seed length of ClassA is significantly longer than ClassB.

4, the functional verification of GL3-3: utilize overexpression technology, by agriculture bacillus mediated genetic transformation, find this baseBecause can making the grain length of transformation receptor paddy rice " Japan is fine ", the rising of expression rises to 7.81mm from 7.22mm;Mass of 1000 kernel is increased to 23.25g from 21.14g, reaches extremely significant difference.

Brief description of the drawings

Fig. 1 is the molecular labeling schematic diagram of paddy rice trisome location

SSR flag sequence is referring to http://www.gramene.org/.

Fig. 2 is the haplotype analysis result figure of GL3-3

There are to analyze according to system four kinds of haplotype cluster analysis result schematic diagrames that obtain in Fig. 3.

What Fig. 4 was the ClassA that obtains of four kinds of haplotype cluster analyses and the large class of ClassB two in seed length is poorDifferent comparison diagram

Fig. 5 is expression vector pMDC32 collection of illustrative plates

Fig. 6 turns GL3-3 rice plant in PCR qualification T0 generation

Fig. 7 is the real-time fluorescence quantitative PCR testing result that turns OsGL3-3 rice plant

Fig. 8 turns the grain length of OsGL3-3 paddy rice and the heavy phenotype difference comparison diagram with negative control plant of grain in T2 generation

Fig. 9 turns the phenotype of OsGL3-3 paddy rice T2 for seed in T2 generation

Detailed description of the invention

The experimental technique using in following embodiment if no special instructions, is conventional method.

Material, reagent etc. used in following embodiment, if no special instructions, all can obtain from commercial channels.

Rice varieties SLG-1 is documented in as in Publication about Document: Ma Lilian etc. rice big grain germplasm resource and genetic analysis. plantThing is learned circular, 2006,23 (4): 395-401; The public can obtain from China Agricultural University.

Fine being documented in as in Publication about Document of rice varieties Japan: rice varieties " Japan is fine ". agricultural science and technology communication .197302 phase; The public can obtain from China Agricultural University.

Carrier pMDC32 is documented in as in Publication about Document: EvaM.Farre ' andSteveA.Kay.PRR7proteinlevelsareregulatedbylightandthecircadianclockinArabidopsis.ThePlantJournal, 2007,52:548 – 560; The public can obtain from China Agricultural University, and the result schematic diagram of this carrier isFig. 5.

Agrobacterium tumefaciems EHA105 is documented in as in Publication about Document: Gao Shiwu etc. affect Agrobacterium tumefaciems EHA105 competenceThe research of cell transformation efficiency factor. tropical biological journal .2012 the 3rd the 1st phase of volume of March; The public can be from Chinese agricultureSparetime university learns and obtains.

The acquisition of embodiment 1, GL3-3 albumen and encoding gene thereof

1, the location of GL3-3 gene

Utilize one by the especially big grain of paddy rice kind SLG (donor parents) and granule kind Japan fine (Nipponbare,Recurrent parent) build the inbred strais that backcrosses (BILs) BC4F2 of colony totally 175 strains, investigate after each strain maturationMass of 1000 kernel, grain length, grain wide and grain is thick, as the phenotypic data of QTL location. Meanwhile, use BSA method from evenlyBe distributed in the polymorphism mark filtering out in 1513 pairs of SSR marks on 12 chromosomes of paddy rice, marker bands is with largeGrain parent SLG is consistent, is designated as A, fine consistent with Japan, is designated as B, and heterozygosis is designated as H. Utilize Mapmaker3.0 to enterRow labels linkage mapping, Group command packet, Kosambi method is calculated genetic distance. QTL scanning adopts QTLIciMapping3.0 (Wang Jiankang etc., 2009), the threshold value existing as QTL using LOD value 2.5, calculates and adds simultaneouslyProperty and dominant effect. The nomenclature principle of QTL adopts the naming methods such as McCouch, and linkage inheritance figure adopts Liu etc. to paintDrawing method completes in EXCEL2010. In the particle shape QTL detecting, the gene places of much having cloned are coveredRegion, for example grain length GS3 (Fanetal., 2006), the wide GW2 of grain (Songetal., 2007) and GW8 (WangEtal., 2012) etc. Meanwhile, near No. three chromosomal end mark RM1278, scan a new controlSeed length and the heavy QTL of grain, effect value reaches 19%. In addition, in multiple familys such as BC4F4, all scan thisQTL。

Utilize approximately 2000 strain NIL-F2 colonies of village experiment station plantation in Beijing China Agricultural University in 2013 to carry out essenceThin location, target gene is positioned in the interval of about 280kb between common indicium RM14484 and RM1278 (asFig. 1).

2, determining of the association analysis of candidate region and candidate gene

Obtain candidate region by the high accuracy order-checking to 272 parts of paddy rice Mini core collection resource 14X wherein high closeThe SNP data (having 7 SNP in average 1kb) of degree, association analysis is carried out in the candidate region behind meticulous location (to be hadGroup structure is carried out colony's rectification as covariant). In highly associated SNP, there are 19 concentrated genes that are distributed inThe promoter region of Os03g0183000. Comparison sequencing analysis by this gene region finds, fine with Japan compared with,In SLG, there is these SNP sites. The encoding proteins (nucleotides sequence is classified sequence 3 as) of this gene have one conservativeAP2 domain, belongs to AP2/EREBP class transcription factor family. AP2/EREBP class transcription factor member in arabidopsisAP2, participates in uncertainty and ovule and the seed coat establishing floral organ and floral meristem feature, suppress floral meristemGrowth (Ohtoetal.2005); Ohto etc. also think that arabidopsis AP2 regulates and controls by affecting glycometabolismThe size of seed. In addition, also find to have much and growth and development of plants phase at Os03g0183000 gene promoter regionThe cis-acting elements closing. Therefore, this gene is defined as to candidate gene GL3-3. The promoter region core of GL3-3 geneNucleotide sequence is sequence 1, and full-length cDNA nucleotides sequence is classified sequence 2 in sequence table as, the albumen name of this gene codeFor GL3-3, its amino acid sequence is sequence 3 in sequence table.

3, the haplotype analysis of GL3-3

According to 5 associated sites the most significant (comprising 4 SNP and 5 '-indel) of GL3-3 promoter region247 parts of Mini core collections of paddy rice that this seminar is collected carry out haplotype phenotypic analysis, found that, they canBe divided into four kinds of haplotypes (Fig. 2); Use software MEGA5 to carry out system and analyze, these four kinds of haplotypes again canBe divided into ClassA and the large class of ClassB two; And the seed length of ClassA is significantly longer than ClassB and (is seen Fig. 3With 4).

The functional verification of embodiment 2, GL3-3 gene

One, turn the acquisition of GL3-3 paddy rice

1, the acquisition of recombinant vector

Get the fine seedling of the Japan of cultivating under normal condition, Trizol method is extracted total RNA, uses M-MLV reverse transcription after purifyingEnzyme carries out reverse transcription and obtains cDNA. Taking this cDNA as template, design primers F 1 and primer R1 carry out pcr amplification,Amplified production is carried out to agarose gel electrophoresis, and the DNA fragmentation that reclaims purifying 1580bp checks order.

This PCR product of result has sequence table sequence 2 from 5 ' end 10-1570 position nucleotides, wherein from 5 ' endHold the 102nd to the 1106th ORFs that is GL3-3 gene.

The sequence of above-mentioned primer is as follows:

F1：5'-CGGGGTACCAAAGGCATTCGCAACACACA-3'(underscore base (4-9bp) is restrictedThe restriction endonuclease recognition sequence of restriction endonuclease KpnI);

R1：5'-CCTTAATTAACCAAAATACATTACGACTGGAC-3'(underscore base (1571-1578bp)For the restriction endonuclease recognition sequence of restriction enzyme PacI).

By KpnI and PacI double digestion for the PCR product of above-mentioned acquisition 1580bp, the enzyme obtaining cut product with through processThe skeleton fragment of the carrier pMDC32 that same enzyme is cut is connected, and obtains recombinant vector.

Through order-checking, this recombinant vector is that sequence table sequence 2 is inserted from 5 ' end 10-1570 position nucleotidesThe carrier that between the KpnI of pMDC32 carrier and PacI site, (i.e. two tobacco mosaic disease virus promoter 35S downstream) obtains,Called after 35S::GL3-3.

2, the acquisition of restructuring Agrobacterium

Above-mentioned recombinant vector 35S::GL3-3 freeze-thaw method is transformed to Agrobacterium tumefaciems EHA105, obtain and contain recombinant vectorThe Agrobacterium tumefaciems EHA105 of 35S::GL3-3, Agrobacterium EHA105/35S::GL3-3 recombinates.

The plasmid that extracts restructuring Agrobacterium EHA105/35S::GL3-3, KpnI and PacI enzyme are cut qualification, obtain 1580bp,Positive restructuring Agrobacterium.

3, the acquisition of genetically modified plants and qualification

Infect paddy rice Japan fine (below also referred to as wild type paddy rice) with above-mentioned restructuring Agrobacterium EHA105/35S::GL3-3Embryo callus, obtain 8 T0 generation and turn GL3-3 rice strain, concrete grammar is as follows:

1), infect the preparation of liquid

Restructuring Agrobacterium EHA105/35S::GL3-3 is laid in containing 50mg/L kanamycins and 20mg/L rifampinIn YEP culture medium, cultivate 2-3 days for 28 DEG C. Picking list bacterial plaque be inoculated in YEP fluid nutrient medium (50mg/L card thatMycin and 20mg/L rifampin), 28 DEG C, it is 0.8-1.0 that 240rpm is cultured to OD600, by 1% inoculum concentration inoculationIn YEP fluid nutrient medium (50mg/L kanamycins and 20mg/L rifampin), 28 DEG C, 240rpm is cultured to OD600For 0.5-0.6, centrifugal collection thalline is resuspended in AAM culture medium 28 DEG C, and it is 0.3-0.4 that 240rpm is cultured to OD600As infecting liquid.

2), infect and be total to cultivation

Choose the fine embryo callus step 1 of good paddy rice Japan) immersion that infects prepared steeps after 30 minutes and getsGo out, suck unnecessary bacterium liquid with aseptic filter paper, be then placed in common culture medium and cultivate 2-3 days.

3), screening and culturing

Will be through step 2) the callus sterilized water oscillation cleaning cultivated altogether 3-4 time, then use 500mg/L cephalo mouldElement aqueous solution vibration washing 40 minutes, until supernatant is completely clean; Take out callus, put into and be only with filter paperSterile petri dish in air-dry 4 hours of 0.4m/s; Proceed to and postpone screening and culturing base and secretly cultivate after 3-7 days and proceed to again screeningIn culture medium, screen two-wheeled (the every 3-4 of wheel week).

4), differentiation is cultivated and is obtained transfer-gen plant

Will be through step 3) callus dark cultivation after 2-3 week in pre-differential medium of cultivating, then move on to differentiation trainingSupport illumination cultivation 2-3 week in base, in the time that young shoot grows to about 1cM, proceed to strong seedling culture base and cultivate 30 days, throw off sealed membraneHardening is cultivated one week, is then transplanted in soil, obtains T0 for turning GL3-3 paddy rice.

Above-mentioned culture medium prescription is as shown in table 2.

Table 2 is culture medium prescription

Note: NB culture medium basis comprises N6 a great number of elements, B5 trace element, B5 organic principle, 150mg/L inositol,300mg/L caseinhydrolysate, 500mg/L glutamine, 600mg/L proline, 30g/L sucrose, 3g/L plant is solidifyingGlue.

5) PCR qualification

To step 4) T0 generation of obtaining turns GL3-3 rice plant seedling and extracts RNA, and reverse transcription obtains cDNA as mouldPlate, with primer 5 '-AAAAGTTCGACAGCGTCTCCGACC-3 ' and 5 '-TCTACACAGCCATCGGTCCAGACG-3 ' carries out pcr amplification. Taking wild type paddy rice as contrast.

As shown in Figure 6, p is plasmid 35S::GL3-3 to result, and W is wild type paddy rice, and 1-8 turns GL3-3 in T0 generationPaddy rice, can find out, the positive T0 generation of object fragment that obtains 919bp turns GL3-3 paddy rice.

Obtain altogether 8 T0 for turning GL3-3 rice strain.

Transgenosis plant in the present age is shown in T0 representative, and the seed that T0 produces for selfing and the plant being grown up to by it are shown in T1 representative,The seed that T1 produces for selfing and the plant being grown up to by it are shown in T2 representative, and the seed that T2 produces for selfing is shown in T3 representativeAnd the plant being grown up to by it.

In T0 generation, turns the OE4 of GL3-3 rice strain, OE5 planting seed, cultivates, and turns the strain of GL3-3 paddy rice until obtain T2Be OE4, OE5.

Adopt and use the same method, empty carrier pMDC32 is proceeded to wild type paddy rice, obtain T0 for turning empty carrier paddy rice,Cultivate, obtain T2 for turning empty carrier paddy rice.

4, the real-time fluorescence quantitative PCR that turns GL3-3 paddy rice detects

Fine wild type Japan (WT), T2 are turned to the OE4 of GL3-3 rice strain, OE5 at field planting, each strain 10Strain. Extract respectively each strain plant leaf, utilize TRIZOL reagent to extract total RNA, taking this RNA as template, useM-MLV reverse transcriptase is carried out reverse transcription and is obtained cDNA, taking this cDNA as template, and employing primer 5 '-ATGGCTTGCTTGATTACCGAA-3 ' and 5 '-AGACCCCGTAAAAGTAGCCCA-3 ' increases, amplificationThe specific fragment (142bp) of GL3-3 gene.

Taking Actin gene as contrast, the primer of this gene that increases is 5 '-ATTTGGCACCACACATTCTAC-3 'With 5 '-ATAACCTTCGTAGATTGGGACT-3 ', amplify the specific fragment (255bp) of paddy rice Actin geneTo carry out real-time quantitative analysis as internal reference.

Real-time fluorescence quantitative PCR is at real-time fluorescence quantitative PCR instrument AppliedBiosystems7500RealTimePCROn system (ABI, USA), carry out, 3 repetitions are established in a parallel test. Utilize LivakKJ and SchmittgenThe method of TD (2001) report, 2-Δ Δ CT calculates relative expression quantity.

ΔΔCT＝(CT.Target－CT.Actin)Timex－(CT.Target－CT.Actin)Time0

Timex represents random time point, and Time0 represents the target gene expression of 1 times of amount after actin proofreaies and correct.

As shown in Figure 7, compared with wild type paddy rice, T2 turns GL3-3 in the OE4 of GL3-3 rice strain, OE5 to resultThe expression of gene is apparently higher than wild type paddy rice.

Two, turn GL3-3 paddy rice phenotype

Fine wild type Japan (WT), T2 are turned to the OE4 of GL3-3 rice strain and T2 generation and turn empty carrier Rice Cropping land for growing field cropsAfter carry out Phenotypic Observation, each strain 20 strains, experiment in triplicate, results averaged.

Add up each strain paddy rice grain length and mass of 1000 kernel, result as shown in Figure 8,

Grain length and the mass of 1000 kernel of wild type Japan fine (WT) are respectively 7.22mm and 21.14g;

Grain length and mass of 1000 kernel that T2 turns the OE4 of GL3-3 rice strain (OE) are respectively 7.81mm and 23.25g;

Observe the seed phenotypes that T2 turns the OE4 of GL3-3 rice strain, result is as Fig. 9, in figure: above arranging grain is T2Turn GL3-3 rice strain, lower row's grain is the fine contrast of wild type Japan, can find out, with wild type Japan fine (WT)Compare, the grain length that T2 turns the OE4 of GL3-3 rice strain (OE) significantly increases.

In wild type Japan fine (WT) and T2 generation, turn empty carrier paddy rice result without significant difference.

Therefore, can find out, GL3-3 gene can improve rice yield, by improving paddy rice grain length and mass of 1000 kernel bodyExisting.

Claims (10)

1. an albumen is following 1) or 2) protein:

1) protein being formed by the amino acid residue shown in sequence in sequence table 3;

2) by 1) shown in protein amino acid residue sequence through the replacement of one or several amino acid residue and/Or disappearance and/or add and have identical function by 1) derivative protein.

2. the DNA molecular of albumen described in coding claim 1.

3. DNA molecular according to claim 2, is characterized in that: described DNA molecular is following 1)-5)In arbitrary described DNA molecular:

1) code area is the DNA molecular shown in sequence 2 in sequence table;

2) code area be in sequence table sequence 2 from 5 ' end 10-1570 position nucleotides;

3) code area be in sequence table sequence 2 from 5 ' end 102-1106 position nucleotides;

4) under stringent condition with 1) or 2) or 3) hybridization and coding have the DNA molecular of identical function albumen;

5) with 1) or 2) or 3) 90% above homology and coding there is and have the DNA molecular of identical function albumen.

4. contain described in claim 2 or 3 expression cassette, recombinant vector, recombinant bacterium, the transgenosis of DNA molecular thinBorn of the same parents system or recombinant bacterium.

5. recombinant vector according to claim 4, is characterized in that: described recombinant vector is by claim 2Or described in 3, DNA molecular inserts the recombinant vector that expression vector obtains.

6. recombinant bacterium according to claim 4, is characterized in that: described recombinant bacterium is by described recombinant vectorImport the recombinant bacterium obtaining in object bacterium.

DNA molecular described in albumen claimed in claim 1, claim 2 or 3, contain claim 2 or 3Expression cassette, recombinant vector, recombinant bacterium, transgenic cell line or the recombinant bacterium of described DNA molecular are in regulating plant outputIn application.

8. application according to claim 7, is characterized in that:

Described output heavily embodies by seed grain length and/or grain;

Described regulating plant output is that raising plant seed grain length and/or grain are heavy;

Described plant is specially monocotyledon or dicotyledon; Described monocotyledon is specially paddy rice.

9. cultivate a method for genetically modified plants, plant for DNA molecular described in claim 2 or 3 is imported to objectThing, obtains genetically modified plants;

Described genetically modified plants have following 1) and/or 2) feature:

1) the seed grain length of described genetically modified plants is greater than described object plant;

2) the seed grain of described genetically modified plants is great in described object plant;

Described DNA molecular imports object plant by recombinant vector described in claim 4;

Described plant is specially monocotyledon or dicotyledon; Described monocotyledon is specially paddy rice.

DNA molecular described in albumen claimed in claim 1, claim 2 or 3, contain claim 2 orDescribed in 3, the expression cassette of DNA molecular, recombinant vector, recombinant bacterium, transgenic cell line or recombinant bacterium are planted at cultivation high yieldApplication in thing; Described plant is specially monocotyledon or dicotyledon; Described monocotyledon is specially paddy rice.