CN105582600B - A kind of insect micro-injection method based on manual microinjector - Google Patents
A kind of insect micro-injection method based on manual microinjector Download PDFInfo
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- CN105582600B CN105582600B CN201510936491.5A CN201510936491A CN105582600B CN 105582600 B CN105582600 B CN 105582600B CN 201510936491 A CN201510936491 A CN 201510936491A CN 105582600 B CN105582600 B CN 105582600B
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- Prior art keywords
- injection
- insect
- needle
- injection needle
- tube
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
- A61M5/31—Details
- A61M5/315—Pistons; Piston-rods; Guiding, blocking or restricting the movement of the rod or piston; Appliances on the rod for facilitating dosing ; Dosing mechanisms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
- A61M5/31—Details
- A61M5/315—Pistons; Piston-rods; Guiding, blocking or restricting the movement of the rod or piston; Appliances on the rod for facilitating dosing ; Dosing mechanisms
- A61M5/31525—Dosing
- A61M5/31531—Microsyringes, e.g. having piston bore diameter close or equal to needle shaft diameter
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2250/00—Specially adapted for animals
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- Health & Medical Sciences (AREA)
- Vascular Medicine (AREA)
- Engineering & Computer Science (AREA)
- Anesthesiology (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Hematology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
- Catching Or Destruction (AREA)
Abstract
The present invention provides a kind of insect micro-injection method based on manual microinjector, belongs to insect physiological and biochemical research field.This method is by means of manual microinjector, the micro-injection that allogene is carried out to insect may be implemented, this micro-injection method is compared with the micro-injection method by trace injection instrument, without expensive trace injection instrument, used manual microinjector structure is simple, is easy to carry about with one, stores and changes, injection experiment can be not limited to fixed location progress, and injection process is simple and efficient.
Description
Technical field
The present invention relates to a kind of micro-injection methods, especially suitable for carrying out the micro-injection of allogene to insect.
Background technique
Insect is the animal of nature most species, and the production activity and health with the mankind are closely related.Study elder brother
The physiological and biochemical property of worm is the basis for utilizing or controlling insect.The micro-injection of insect refers to will be micro by the equipment or instrument
It is one of the important technology of insect physiological and biochemical research in liquid reagent injection insect bodies.Micro note is carried out to insect at present
It penetrates and tends to rely on trace injection instrument, although trace injection instrument can reach preferable injection effect, be to rely on micro-injection
There are three defects for the micro-injection method of instrument: (1) trace injection instrument is more expensive, not universal in most laboratory；(2) micro-injection
Instrument relies on power supply, and mostly injection experiment dependent on trace injection instrument is carried out in fixed laboratory, injects the place of experiment
General bad change；(3) though the micro-injection dependent on trace injection instrument is uncomplicated, operator generally requires a timing
Between training could grasp very well.Therefore, needs exploitation at present does not depend on trace injection instrument and insect easy to spread is micro
Injecting method.
Summary of the invention
It is an object of the invention to be directed to the deficiency of existing insect microinjection technique, provide a kind of based on manual micro note
The insect micro-injection method of emitter.
The present invention provides a kind of manual microinjectors, are achieved through the following technical solutions: by injection needle, sealant
Plug, rubber plug outer tube, rubber tube, connecting tube and spherical Capsule composition, injection needle are inserted into the bellmouth of seal rubber plug, sealant
In plug insertion rubber plug outer tube, the front end of outer tube rear end connection rubber tube, the front end use of the rear end of rubber tube and spherical Capsule
Connecting tube is connected；The injection needle draws needle to form by the transparent glass capillary that outer diameter is 1.0 mm, has scale, minimum scale institute
Liquid volume is 0.1 μ L in the needle represented；The seal rubber plug is rubber stopper, and tool is elastic, there is an inner concave shape cone among rubber plug
Shape hole, bellmouth minimum-value aperture are 0.8 mm, less than the outer diameter of the injection needle, are guaranteed closed between injection needle and seal rubber plug
Property；It is the stomata of 6 mm that, which there is a diameter in the spherical shape Capsule rear end,；The injection needle, seal rubber plug, rubber plug outer tube, rubber
Pipe, connecting tube and spherical Capsule are detachable, replace.
The present invention provides a kind of micro-injection method, this method is based on above-mentioned manual microinjector, including following step
It is rapid:
1) it benumbs insect: the insect of collection is benumbed 5 minutes on ice；
2) preparation of syringe and injection: the rear end of the rubber tube of above-mentioned manual microinjector is taken from connecting tube
Under, it is attached on the syringe-type syringe sold market, the syringe needle of injection needle is submerged into solution, the work of pull syringe-type syringe
Plug slowly sucks liquid to be injected in injection needle, and then the rear end of rubber tube is connected in connecting tube, completes syringe
With the preparation of injection；
3) injection experiment: the insect benumbed being moved under disecting microscope, carefully fixes insect with blunt nosed tweezers,
The syringe needle of the injection needle of manual microinjector described in claim 1 is protruded into the insect body fixed, with finger pressure
The firmly stomata of spherical Capsule rear end, pressure ball shape Capsule forward, with the flowing of manual microinjector pipeline interior air-flow, liquid from
The syringe needle of injection needle flows out, and into insect bodies, completes injection.Required liquid in injection needle is injected into insect bodies
Afterwards, the syringe needle of injection needle is slowly removed, injection is completed, after insect after reviving in paralysis, insect is moved in dependent insect cage and is raised.
After injection needle removes polypide, the finger for pushing down stomata is unclamped, air-flow enters in pipeline from stomata, and spherical Capsule restores former
Sample, and the liquid in injection needle is because the tension force effect of water maintains original position, in case injection next time.Repeatedly, right
Bull insect is continuously injected.
Liquid to be injected is injected into insect bodies by the method for the present invention using manual microinjector, is realized to the micro- of insect
Amount injection.Insect micro-injection method of the present invention, used manual microinjector structure is simple, be easy to carry about with one,
It stores and changes, injection experiment can be not limited to fixed location progress, and injection process is simple and efficient.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of heretofore described manual microinjector.In figure, 1 it is injection needle, 2 is sealing
Rubber plug, 3 be rubber plug outer tube, 4 be rubber tube, 5 be connecting tube, 6 be spherical Capsule, 7 be stomata, 8 be bellmouth.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are only used for explaining this hair
It is bright, rather than limit the scope of the invention.Under the premise of without departing substantially from technical solution of the present invention, made for the present invention
Field those of ordinary skill any change easy to accomplish is fallen within scope of the presently claimed invention.
A kind of embodiment one: manual microinjector
A kind of manual microinjector structure that micro-injection method of the present invention is relied on is as follows: as shown in Figure 1,
It is made of injection needle 1, seal rubber plug 2, rubber plug outer tube 3, rubber tube 4, connecting tube 5 and spherical Capsule 6；The injection needle 1 is inserted
Enter in the inner concave shape bellmouth 8 of seal rubber plug 2, seal rubber plug 2 is embedded in rubber plug outer tube 3, and the rear end of rubber plug outer tube 3 connects
The front end of rubber tube 4, the rear end of rubber tube 4 are connected with the front end connecting tube 5 of spherical Capsule 6；1 outer diameter of injection needle is
1.0 mm have scale, and liquid volume is 0.1 μ L in needle representated by minimum scale；Inner concave shape cone in the seal rubber plug 2
The minimum-value aperture in shape hole 8 is 0.8 mm；It is the stomata 7 of 6 mm that the rear end of the spherical shape Capsule, which has an aperture,；Injection needle 1,
Seal rubber plug 2, rubber plug outer tube 3, rubber tube 4, connecting tube 5 and spherical Capsule 6 are detachable, replace.
A kind of embodiment two: insect micro-injection method
For this method dependent on manual microinjector described in embodiment one, specific implementation step is as follows:
1) it benumbs insect: the insect of collection is benumbed 5 minutes on ice；
2) preparation of syringe and injection: after the rubber tube 4 of manual microinjector described in embodiment one
End is removed from connecting tube 5, is attached on the syringe-type syringe sold market, the syringe needle of injection needle 1 is submerged solution, pull needle
The piston of cartridge type syringe slowly sucks liquid to be injected in injection needle 1, and the rear end of rubber tube 4 is then connected to connection
On pipe 5, the preparation of syringe and injection is completed；
3) injection experiment: the insect benumbed is moved under disecting microscope, carefully fixes insect with blunt nosed tweezers,
The syringe needle of the injection needle 1 of manual microinjector described in claim 1 is protruded into the insect body fixed, with finger pressure
The firmly rear end stomata 7 of spherical Capsule 6, pressure ball shape Capsule 6 forward, with the flowing of manual microinjector pipeline interior air-flow, liquid
Body is flowed out from the syringe needle of injection needle 1, into insect bodies, completes injection.Required liquid in injection needle 1 is injected into insect
After in vivo, the syringe needle of injection needle 1 is slowly removed, completes injection, after insect after reviving in paralysis, insect is moved into dependent insect cage and is raised
It supports.After injection needle 1 removes polypide, the finger for pushing down stomata 7 is unclamped, air-flow enters in pipeline from stomata 7, spherical Capsule 6
Restore as former state, and the liquid in injection needle 1 is because the tension force effect of water maintains original position, in case injection next time.So
Repeatedly, bull insect is continuously injected.
Claims (1)
1. a kind of insect micro-injection method based on manual microinjector, it is characterised in that the manual microinjector used
Structure is as follows: being made of injection needle, seal rubber plug, rubber plug outer tube, rubber tube, connecting tube and spherical Capsule, injection needle insertion
In the bellmouth of seal rubber plug, seal rubber plug is embedded in rubber plug outer tube, the front end of the rear end connection rubber tube of rubber plug outer tube,
The rear end of rubber tube is connected with the front end of spherical Capsule with connecting tube, and the injection needle is that 1.0 mm have the transparent of scale by outer diameter
Capillary glass tube draws needle to form, and liquid volume is 0.1 μ L in needle representated by minimum scale, and the seal rubber plug is rubber stopper,
Tool is elastic, there is an inner concave shape bellmouth among rubber plug, and bellmouth minimum-value aperture is 0.8 mm, less than the outer diameter of injection needle, protects
The airtightness between injection needle and seal rubber plug is demonstrate,proved, it is the stomata of 6 mm that, which there is a diameter in the spherical shape Capsule rear end, described
Injection needle, seal rubber plug, rubber plug outer tube, rubber tube, connecting tube and spherical Capsule are detachable, replace；The insect is micro-
Injecting method is measured, it is further characterized in that including following operating procedure:
(1) it benumbs insect: the insect of collection is benumbed 5 minutes on ice；
(2) preparation of syringe and injection: the rear end of the rubber tube of the manual microinjector is removed from connecting tube,
It is attached on the syringe-type syringe sold market, the syringe needle of injection needle is submerged into solution, the piston of pull syringe-type syringe
Liquid to be injected is slowly sucked in injection needle, then the rear end of rubber tube is connected in connecting tube, complete syringe and
The preparation of injection；
(3) injection experiment: the insect benumbed being moved under disecting microscope, carefully fixes insect with blunt nosed tweezers, will
The syringe needle of the injection needle of the manual microinjector protrudes into the insect body fixed, after pushing down spherical Capsule with finger
The stomata at end, pressure ball shape Capsule forward, with the flowing of manual microinjector pipeline interior air-flow, syringe needle of the liquid from injection needle
Outflow completes injection into insect bodies；
(4) after the required liquid in injection needle is injected into insect bodies, the syringe needle of injection needle is slowly removed, completes injection,
After insect after reviving in paralysis, insect is moved into dependent insect cage raising；
(5) after injection needle removes polypide, the finger for pushing down stomata is unclamped, air-flow enters in pipeline from stomata, spherical Capsule
Restore as former state, and the liquid in injection needle is because the tension force effect of water maintains original position, in case injection next time；
(6) repeatedly, bull insect can continuously be injected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510936491.5A CN105582600B (en) | 2015-12-16 | 2015-12-16 | A kind of insect micro-injection method based on manual microinjector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510936491.5A CN105582600B (en) | 2015-12-16 | 2015-12-16 | A kind of insect micro-injection method based on manual microinjector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105582600A CN105582600A (en) | 2016-05-18 |
| CN105582600B true CN105582600B (en) | 2019-04-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510936491.5A Expired - Fee Related CN105582600B (en) | 2015-12-16 | 2015-12-16 | A kind of insect micro-injection method based on manual microinjector |
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| Country | Link |
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| CN (1) | CN105582600B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111264475B (en) * | 2020-03-09 | 2021-11-19 | 沈阳农业大学 | Microinjection method for small insect adults of whiteflies |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2122606U (en) * | 1991-12-31 | 1992-11-25 | 巴图 | Veterinary rapid transfusion system |
| CN202288530U (en) * | 2011-10-17 | 2012-07-04 | 浙江大学舟山海洋研究中心 | Microinjection needle for shrimps and crabs |
| CN102648877A (en) * | 2012-04-25 | 2012-08-29 | 中国人民解放军第四军医大学 | Digital adjustable microinjection method |
| CN203001171U (en) * | 2013-01-16 | 2013-06-19 | 哈尔滨医科大学附属第四医院 | Animal temporal-mandibular joint microinjector |
| CN103800095A (en) * | 2014-02-27 | 2014-05-21 | 福建农林大学 | Low-fatality-rate micro-injection method for leafhopper |
| CN104173118A (en) * | 2014-08-22 | 2014-12-03 | 山西省肿瘤研究所 | Device for establishing rectal transplanted tumor in mice |
| CN204106251U (en) * | 2014-10-14 | 2015-01-21 | 黑龙江省兽医科学研究所 | The oral transfusion device of house pet |
| CN204246268U (en) * | 2014-11-18 | 2015-04-08 | 东南大学 | Experiment mice common bile duct injection device |
-
2015
- 2015-12-16 CN CN201510936491.5A patent/CN105582600B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2122606U (en) * | 1991-12-31 | 1992-11-25 | 巴图 | Veterinary rapid transfusion system |
| CN202288530U (en) * | 2011-10-17 | 2012-07-04 | 浙江大学舟山海洋研究中心 | Microinjection needle for shrimps and crabs |
| CN102648877A (en) * | 2012-04-25 | 2012-08-29 | 中国人民解放军第四军医大学 | Digital adjustable microinjection method |
| CN203001171U (en) * | 2013-01-16 | 2013-06-19 | 哈尔滨医科大学附属第四医院 | Animal temporal-mandibular joint microinjector |
| CN103800095A (en) * | 2014-02-27 | 2014-05-21 | 福建农林大学 | Low-fatality-rate micro-injection method for leafhopper |
| CN104173118A (en) * | 2014-08-22 | 2014-12-03 | 山西省肿瘤研究所 | Device for establishing rectal transplanted tumor in mice |
| CN204106251U (en) * | 2014-10-14 | 2015-01-21 | 黑龙江省兽医科学研究所 | The oral transfusion device of house pet |
| CN204246268U (en) * | 2014-11-18 | 2015-04-08 | 东南大学 | Experiment mice common bile duct injection device |
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| Publication number | Publication date |
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| CN105582600A (en) | 2016-05-18 |
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Address after: Hangzhou City, Zhejiang province 310018 Xiasha Higher Education Park source Street No. 258 Applicant after: CHINA JILIANG UNIVERSITY Address before: Hangzhou City, Zhejiang province 310018 Xiasha Higher Education Park source Street No. 258 Applicant before: China Jiliang University |
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