CN103800095A - Low-fatality-rate micro-injection method for leafhopper - Google Patents
Low-fatality-rate micro-injection method for leafhopper Download PDFInfo
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- CN103800095A CN103800095A CN201410067514.9A CN201410067514A CN103800095A CN 103800095 A CN103800095 A CN 103800095A CN 201410067514 A CN201410067514 A CN 201410067514A CN 103800095 A CN103800095 A CN 103800095A
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- leafhopper
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Abstract
The invention provides a low-fatality-rate micro-injection method for leafhopper, and belongs to the field of insect physiology and biochemistry research. The method improves injection efficiency and the survival rate of the leafhopper and reduces manpower and time consumption. Micro-injection on an insect will cause mechanical injuries to an insect body and cause death of the insect body in severe cases, so that requirements for an instrument and technologies are high, and the survival rate of the insect can be affected by the caliber and material of an injection needle, the age and injection position of the insect, an injection reagent and the dosage, an injection angle, injection time, the liquid flow rate and other factors. The survival rate of the leafhopper can reach 55%-100% through the injection method.
Description
Technical field
A kind of leafhopper micro-injection method that the present invention relates to low fatality rate, belongs to insecticide physiological and biochemical research field.
Background technology
Leafhopper is the insecticide of a class evil Cereal, vegetable, fruit tree and forest etc.Homoptera, Cicadellidae.The long 3-15 millimeter of body.This section insecticide not only harms the crops, but also propagates the viroses of plant.The Physiology and biochemistry feature of research leafhopper, is conducive to disclose the relation between insecticide and crops, for insect control provides foundation.
The micro-injection of insecticide refers to by instrument, micro liquid shape reagent injected in insecticide, and be the important method of Entomological Physiology research, be one of most important technology in the experiment of insecticide Physiology and biochemistry.But the method can cause the mechanical damage of insecticide body, when serious, can cause polypide death, therefore having relatively high expectations to instrument and technology, entry needle bore, material, the length of time of insecticide and injection site, injection reagent and dosage, and the factor such as injection angles, time and flow rate of liquid all can affect the survival rate of insecticide.At present trace injection instrument mainly contain produce hydraulic pressure oil pump, produce the air pressure pump of air pressure etc., volume injected scope is raised to and receives liter from skin.Although instrument is meticulousr, the injury that insecticide is caused may be less, and micro-injection is still higher to operation and material requirements, as 1) bore after entry needle drawing, bore is larger, and the wound causing is larger; 2) insecticide length of time, the larger insecticide of polypide is after injection, and wound easily heals, and survival rate is higher, but the little insecticide of polypide, survival rate is low; 3) injection site, mostly is the coria of abdominal part, and angle is also depending on operator's experience.Therefore affect this technology because have artificial and material itself, but its final purpose is all the injury reducing as far as possible insecticide, guarantees the survival rate of insecticide, so that well the carrying out of subsequent experimental.
Summary of the invention
The object of the present invention is to provide a kind of leafhopper micro-injection method of low fatality rate, improve injection efficiency, reduce manpower and the consumption of time.
For achieving the above object, the present invention adopts following technical scheme:
A leafhopper micro-injection method for low fatality rate, is characterized in that: comprise the following steps:
1) use and vertically draw pin instrument to draw capillary tube glass entry needle, and check under the microscope syringe needle, guarantee that the glass of scorification does not stop up syringe needle;
2) leafhopper is collected in vitro, benumbs 5-7 minute on ice, the tubule of micro-loading pin front end is cut, for the leafhopper after picking injection; Flatten remaining segment tubule head simultaneously, withstand leafhopper when entering pin;
3) give glass entry needle loading, guarantee after syringe needle contact liq fluid at once, then, adjusting injection needle angle is 30-40 °;
4) postparalytic leafhopper is moved under microscope, upward, mobile syringe needle, by the coria center position between tip alignment metathorax and promerous for abdominal part; With living leafhopper head in the micro-loading pin tubule crown after flattening, while preventing into pin, promote polypide, then carefully enter pin, after needle point thrusts polypide, adjust the oil pressure pump of micrurgy instrument to control injected dose, minimum injected dose is 2 to receive liter, maximum injection dosage is 0.2 microlitre, then leafhopper is dialled gently from syringe needle, and dip in water adhesion leafhopper, carefully move into small-sized rearging cage;
5), after leafhopper revives, move into the conventional raising in insectary.
Described micro-loading pin is microloader trace loading pin.
Described injected dose is not for being 2 to receive and rise-0.2 microlitre, and lethal.
In described step 4), with the tubule that rice seedlings or the micro-loading pin of needle phase are cut, leafhopper is dialled gently from syringe needle.
The invention has the advantages that: injection site easily enters pin, injection angles is less to polypide damage, and injected dose reaches maximum and still can keep compared with high viability, reduces manpower and the consumption of time.
The specific embodiment
Material: rice seedlings, small-sized rearging cage, ice, test tube, capillary tube glass entry needle (internal diameter 90mm), microloader trace loading pin (eppendoff)
Instrument: eppendoff micrurgy instrument injectman NK2, NARISHIGE vertically draws pin instrument PC-10, stereomicroscope.
embodiment 1
Use NARISHIGE vertically to draw pin instrument to draw capillary tube glass entry needle, and check syringe needle under stereomicroscope, guarantee that the glass of scorification does not stop up syringe needle.
The leafhopper in 2 ages is collected in vitro, benumbs on ice 5 minutes.The tubule of microloader trace loading pin front end is cut, for the leafhopper after picking injection; Flatten remaining segment tubule head simultaneously, withstand leafhopper when entering pin.
Give glass entry needle loading, guarantee after syringe needle contact liq fluid at once.Then, adjust micrurgy instrument needle tubing angle, 30 °.
Postparalytic leafhopper is moved under stereomicroscope, and upward, mobile syringe needle, by the coria center position between tip alignment metathorax and promerous for abdominal part.Live leafhopper head with the microloader trace loading pin tubule crown after flattening, while preventing into pin, promote polypide, then carefully enter pin.After needle point thrusts polypide, adjust the oil pressure pump of micrurgy instrument to control injected dose 0.15 microlitre (when dosage is large, visible polypide swells gradually), leafhopper is dialled gently from syringe needle with the tubule that the micro-loading pin of microloader is cut, and dip in water adhesion leafhopper, carefully move into small-sized rearging cage.
After leafhopper revives, move into the conventional raising in insectary.
embodiment 2
Take rice green leafhopper as example, choose 150 of 1 nymphs in age (body is about as 1-1.5mm), injection distilled water, added up survival rate after 12 days, was about 58.7%.Choose 100 of 3 nymphs in age (body is about as 2.0-2.5mm), injection distilled water, after 7 days, add up survival rate, be about 88%, 12 day after survival rate 74%.Choose each 70 of 5 nymphs in age (body is about as 3.5-4mm) and adult (body is about as 5mm), after 12 days, survival rate is 100%.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. a leafhopper micro-injection method for low fatality rate, is characterized in that: comprise the following steps:
1) use and vertically draw pin instrument to draw capillary tube glass entry needle, and check under the microscope syringe needle, guarantee that the glass of scorification does not stop up syringe needle;
2) leafhopper is collected in vitro, benumbs 5-7 minute on ice, the tubule of micro-loading pin front end is cut, for the leafhopper after picking injection; Flatten remaining segment tubule head simultaneously, withstand leafhopper when entering pin;
3) give glass entry needle loading, guarantee after syringe needle contact liq fluid at once, then, adjusting injection needle angle is 30-40 °;
4) postparalytic leafhopper is moved under microscope, upward, mobile syringe needle, by the coria center position between tip alignment metathorax and promerous for abdominal part; With living leafhopper head in the micro-loading pin tubule crown after flattening, while preventing into pin, promote polypide, then carefully enter pin, after needle point thrusts polypide, adjust the oil pressure pump of micrurgy instrument to control injected dose, minimum injected dose is 2 to receive liter, maximum injection dosage is 0.2 microlitre, then leafhopper is dialled gently from syringe needle, and dip in water adhesion leafhopper, carefully move into small-sized rearging cage;
5), after leafhopper revives, move into the conventional raising in insectary.
2. the leafhopper micro-injection method of a kind of low fatality rate according to claim 1, is characterized in that: described micro-loading pin is microloader trace loading pin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410067514.9A CN103800095A (en) | 2014-02-27 | 2014-02-27 | Low-fatality-rate micro-injection method for leafhopper |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410067514.9A CN103800095A (en) | 2014-02-27 | 2014-02-27 | Low-fatality-rate micro-injection method for leafhopper |
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| CN103800095A true CN103800095A (en) | 2014-05-21 |
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| CN201410067514.9A Pending CN103800095A (en) | 2014-02-27 | 2014-02-27 | Low-fatality-rate micro-injection method for leafhopper |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105582600A (en) * | 2015-12-16 | 2016-05-18 | 中国计量学院 | Insect micro-injection method based on manual microsyringe |
| CN111264475A (en) * | 2020-03-09 | 2020-06-12 | 沈阳农业大学 | Microinjection method for small insect adults of whiteflies |
-
2014
- 2014-02-27 CN CN201410067514.9A patent/CN103800095A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105582600A (en) * | 2015-12-16 | 2016-05-18 | 中国计量学院 | Insect micro-injection method based on manual microsyringe |
| CN105582600B (en) * | 2015-12-16 | 2019-04-05 | 中国计量大学 | A kind of insect micro-injection method based on manual microinjector |
| CN111264475A (en) * | 2020-03-09 | 2020-06-12 | 沈阳农业大学 | Microinjection method for small insect adults of whiteflies |
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Application publication date: 20140521 |