CN104173118A - Device for establishing rectal transplanted tumor in mice - Google Patents

Device for establishing rectal transplanted tumor in mice Download PDF

Info

Publication number
CN104173118A
CN104173118A CN 201410416546 CN201410416546A CN104173118A CN 104173118 A CN104173118 A CN 104173118A CN 201410416546 CN201410416546 CN 201410416546 CN 201410416546 A CN201410416546 A CN 201410416546A CN 104173118 A CN104173118 A CN 104173118A
Authority
CN
China
Prior art keywords
tube
dilating
rectum
needle
fixing tube
Prior art date
Application number
CN 201410416546
Other languages
Chinese (zh)
Inventor
李耀平
任连生
杨喜花
Original Assignee
山西省肿瘤研究所
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 山西省肿瘤研究所 filed Critical 山西省肿瘤研究所
Priority to CN 201410416546 priority Critical patent/CN104173118A/en
Publication of CN104173118A publication Critical patent/CN104173118A/en

Links

Abstract

The invention relates to a device for establishing a rectal transplanted tumor in mice. The device comprises a dilating fixing tube and a microsyringe. The upper portion of the dilating fixing tube is provided with a linear injection hole in the lengthwise direction. When the device is in use, a needle of the microsyringe is inserted into the linear injection hole; the needle is longer than the linear injection hole. A handheld portion having a bent angle with the dilating fixing tube is connected to the tail end of the dilating fixing tube. A bulb is connected to the tail end of the handheld portion. The handheld portion, the dilating fixing tube and a tube head are internally provided with air holes communicated; the bulb is communicated with the tail end of the communicated air holes; a front end opening of the communicated air holes is arranged in the top of the tube head. After the dilating fixing tube is inserted into the rectum, the bulb nipped restores and produces negative pressure so that the rectum is tightly sucked to the joint of the tube head and the dilating fixing tube. A rectal bevel is formed in an area near the joint of the tube head and the dilating fixing tube; the rectum is tightly attached to the front end of the dilating fixing tube; accordingly, the needle of the microsyringe can be easily inserted into the rectum. The device is simple to operate and high in tumor formation rate.

Description

一种建立小鼠直肠移植瘤的装置 Means for establishing mouse xenografts rectum

技术领域 FIELD

[0001] 本发明涉及医疗机械,具体而言,是一种建立小鼠直肠移植瘤的装置。 [0001] The present invention relates to medical equipment, in particular, an apparatus for establishing a rectal mouse xenografts.

背景技术 Background technique

[0002]目前在建立肿瘤动物模型时,大多采用皮下肿瘤细胞注射法。 [0002] Currently when tumors were established animal model, most of the subcutaneous tumor cell injection. 无论是肝癌、胃癌、宫颈癌、肺癌等,均将肿瘤细胞接种于小鼠的皮下或肌肉,形成体表或皮下肿瘤。 Whether liver cancer, stomach cancer, cervical cancer, lung cancer, tumor cells were inoculated subcutaneously or intramuscularly in mice, subcutaneous tumors or formed body. 这样接种形成的肿瘤与临床常见的自然发生的肿瘤在生长环境和病程进展等方面均有较大的差异。 Common clinical tumor and tumor vaccination occurs naturally formed such have a greater difference in the growth environment and the other course of progress. 如果想建立肿瘤原位移植瘤,则多数需要采用手术的方法,比如要建立直肠癌动物模型,需要将腹腔打开,将肿瘤细胞搁于肠壁下,实验难以操作,造成失误较多,且术后护理工作量大,动物死亡率高,也难以建立大量的动物模型供实验使用。 If you want to establish orthotopic xenograft tumor, the majority need surgical methods, such as to create animal models of colorectal cancer, you need to open the abdominal cavity, the tumor cells were put under the intestinal wall, the experiment is difficult to operate, resulting in more mistakes, and surgery after nursing workload, high animal mortality rates, it is difficult to establish a large number of animal model for experimental use.

发明内容 SUMMARY

[0003] 本发明要解决的技术问题是提供一种建立小鼠直肠移植瘤的装置,该装置具有操作简单的特点。 [0003] The present invention is to solve the technical problem to provide a device for rectal establish a mouse xenografts, the apparatus having a simple operation characteristics.

[0004] 为解决以上技术问题,本发明采用的技术方案是: [0004] In order to solve the above technical problem, the technical solution adopted by the invention is:

一种建立小鼠直肠移植瘤的装置,包括一个扩张固定管和微量注射器,扩张固定管前端面下部沿其长度方向凸出形成管头,扩张固定管上部沿其长度方向开设有直线形注射孔,使用时微量注射器的针头插入注射孔,针头长度大于注射孔长度,注射孔的内侧端口位于管头上方的扩张固定管前端面、外侧端口位于扩张固定管的外端面;扩张固定管末端连接一个与扩张固定管具有折弯角的握持部,握持部末端连接气囊,所述的握持部、扩张固定管和管头内部开设有连通的气孔,气孔末端连通气囊,气孔前端出口开设于管头顶面。 An apparatus for the rectum of mice to establish xenograft, comprising a fixed tube and a micro syringe expansion, expandable fixing tube projecting distal end face of the lower portion of the tube head is formed along the longitudinal direction, the expansion of the upper stationary tube defines straight injection ports along its length when using a micro syringe needle is inserted into the injection hole, the needle length greater than the injection hole length, inside the injection hole port located tube expansion retaining tube distal end surface of the head side of the outer port on the outer end faces of expanded stationary tube; expansion fixing tube terminus a and expansion of the fixed tube having a folding angle of the grip portion, the grip portion connected to the end of the airbag, the grip portion, and the expanded fixed tube inner tube defines a first communication hole, the communication hole end of the balloon, an outlet hole opened in the front end head tube surface.

[0005] 使用时,先将扩张固定管前端涂润滑剂,插入小鼠肛门内并固定,将注射器经注射针孔插入,将细胞悬液注入结肠壁下,退出针头,缓缓拔出护张固定管即可。 When the [0005] use, the expandable distal retaining tube coated with a lubricant, is inserted into the anus and secured in mice, the injection needle was inserted into the syringe, the cell suspension was injected into the colon wall, withdraw the needle, slowly pull guard sheets fixed tube can be. 扩张固定管起到固定直肠的作用。 Play a fixed stationary tube expansion rectal action. 在将扩张固定管插入小鼠肛门之前,捏紧气囊,排出空气,扩张固定管插入直肠后,气囊恢复,产生的负压将直肠吸紧于管头与扩张固定管的结合部位。 Before fixing the dilator tube is inserted into the anus mice, those of the airbag, the air is discharged after expansion fixing tube inserted into the rectum, the balloon recovery, the negative pressure generated in the rectal absorption tightly binding site and expansion of the fixed ferrule tube. 管头与扩张固定管的结合部位的附近区域形成一个直肠折角,直肠贴紧扩张固定管前端面,使注射器针头能够容易内探到直肠。 Binding site region near the head pipe is formed with a fixing tube expansion knuckle rectum, rectal dilation fixed tube distal end surface adhesion, the syringe needle can be easily within the probe into the rectum.

[0006] 作为一种优选的方案,注射孔的直径为0.45-0.5mm,长度为8_10mm。 [0006] As a preferred embodiment, the diameter of the injection hole is 0.45-0.5mm, length 8_10mm.

[0007] 作为另一种优选的方案,微量注射器针头直径为0.35-0.45mm,针头长度为14-16mm。 [0007] As another preferred embodiment, a diameter of micro-syringe needles 0.35-0.45mm, the needle length of 14-16mm.

[0008] 采用本发明建立小鼠直肠癌原位移植瘤动物模型具有操作简便,对动物操作小,成瘤率高方便实用的优点,在熟练掌握操作技术的前提下,接种成功率可达100%,手术后不需要进行特殊护理。 [0008] The present invention builds colorectal cancer orthotopic xenograft mouse animal model is simple, of small animals, convenient and practical advantages of having high tumor, master the premise technique, the success rate of 100 vaccination %, after surgery does not require special care. 建成的模型具有与临床常见病例有较高的相似度,对病变局部的病理改变的及转移的观察研究更为直观,是开展结直肠癌防治研究更为适宜的动物模型。 The model is built with common clinical cases have high similarity, observational study of local pathological changes of the lesion and the transfer of more intuitive, more prevention research is to carry out an appropriate animal model of colorectal cancer.

[0009] [0009]

附图说明 BRIEF DESCRIPTION

[0010] 图1是本发明建立小鼠直肠移植瘤的装置的结构示意图。 [0010] FIG. 1 is a structural diagram of apparatus of the present invention to establish xenografts in mice rectum.

[0011 ] 图中,1-扩张固定管,2-微量注射器,3-管头,4-注射孔,5-握持部,6-气囊,7_气孔。 [0011] FIG, 1 fixed tube expansion, microinjector 2-, 3- ferrule, the injection hole 4-, 5- grip portion, 6 airbag, 7_ pores.

具体实施方式 Detailed ways

[0012] 如图1所示,本发明要求保护的一种建立小鼠直肠移植瘤的装置,包括一个扩张固定管I和微量注射器2,扩张固定管I前端面下部沿其长度方向凸出形成管头3,扩张固定管I上部沿其长度方向开设有直线形注射孔4,使用时微量注射器的针头插入注射孔4,针头长度大于注射孔4长度,注射孔4的内侧端口位于管头上方的扩张固定管I前端面、夕卜侧端口位于扩张固定管I的外端面;扩张固定管I末端连接一个与扩张固定管具有折弯角的握持部5,握持部5末端连接气囊6,所述的握持部5、扩张固定管I和管头3内部开设有连通的气孔7,气孔7末端连通气囊6,气孔7前端出口开设于管头3顶面。 [0012] As shown in FIG. 1, an apparatus for establishing mouse xenografts rectal claimed invention, comprising a fixed tube expansion and microinjector I 2, I expandable distal retaining tube projecting lower face is formed along the longitudinal direction thereof ferrule 3, the expansion of the fixed pipe upper I defines straight injection ports along its length 4, when using the micro-needle of the syringe is inserted into the injection hole 4, the needle length is greater than the injection hole 4 length, the inner port injection hole 4 of the tube head side expansion of the distal end surface fixed tube I, Xi Bu expansion side port located in the outer end surface of the stationary tube I; I terminus stationary pipe expansion and expansion of a stationary pipe 5 with the grip portion of the bending angle, the end of the grip portion 5 connected to the airbag 6 head 3 inside the grip portion 5, and the pipe expansion I defines fixed tube communicating hole 7, the end of the communication hole 7 balloon 6, the front end of the outlet hole 7 opened in the top surface of the ferrule 3.

[0013] 注射孔4的直径为0.45-0.5mm,长度为8_10mm。 Diameter of [0013] injection hole 4 is 0.45-0.5mm, length 8_10mm. 扩张固定管的直径为3_4mm,管头的长度为3-4mm。 Expanding the diameter of the fixed tube is 3_4mm, the length of the ferrule is 3-4mm.

[0014] 微量注射器针头直径为0.35-0.45mm,针头长度为14_16mm,要求能控制注射量在20-50微升之间,也可用普通的一次性无菌注射器代替。 [0014] The micro syringe needle having a diameter of 0.35-0.45mm, the needle length 14_16mm, required injection amount can be controlled between 20-50 microliters, can also be used in place of conventional disposable sterile syringe. 针头的长度要求非常精确,其申出固定管的长度为4-6mm。 It requires very accurate length of the needle, which apply a fixed length of tube is 4-6mm.

[0015] 以下是采用本发明所述的建立小鼠直肠移植瘤的装置进行的人结肠癌SW480小鼠原位移植瘤动物模型的建立。 [0015] The following means are employed to establish xenografts in mice rectum of the present invention to human colon carcinoma SW480 tumor animal model of orthotopic transplantation in mice.

[0016] 实验动物,SPF级Balb/c裸鼠,雌雄各半,购自北京维通利华实验动物有限公司, 细胞株,人结肠癌细胞株SW480,HCT-116由澳大利亚Newcastle大学张旭东教授赠送, 液体石蜡,北京中联化工试剂厂;PBS,自行配制。 [0016] experimental animals, SPF level Balb / c nude mice, male and female, purchased from Beijing Vital River Laboratory Animal Co., cell lines, human colon cancer cell line SW480, HCT-116 presented by Professor Xudong Newcastle University, Australia , liquid paraffin, United Beijing Chemical reagent factory; PBS, self-prepared.

[0017] 直肠接种器,按照本发明自制,结构及规格如上所述。 [0017] rectal inoculation, made in accordance with the present invention, the structure and size as described above.

[0018] Iml注射器,江西洪达医疗器械集团有限公司,针头规格,0.45 X 16.0mm。 [0018] Iml syringe, Jiangxi HONGDA Medical Devices Group Ltd., needle gauge, 0.45 X 16.0mm.

[0019] 试验方法:SW-480细胞株以含10%胎牛血清的RBMI1640培养基培养48小时后,消化收集细胞,PBS洗I次,调整细胞密度至2.5 X 107/ml,待用。 [0019] Test method: SW-480 cell line containing 10% fetal bovine serum RBMI1640 medium cultured for 48 hours, the cells were harvested, washed I time PBS, cell density was adjusted to 2.5 X 107 / ml, ready for use.

[0020] 操作方法:一人固定小鼠使其处于仰卧位,另一人持直肠接种管,捏紧气囊,在扩张固定管管头蘸少许液体石蜡,从肛门处缓缓插入,插入段全部没入肛门,放松气囊。 [0020] The methods of operation: one mouse so fixed supine position, another person holding the stylus rectum, bladder clenched, liquid paraffin little dip tube is fixed in the expanded head slowly inserted from the anus, anal insertion section fully submerged relax balloon. 注射器抽取细胞悬液,将注射器针头全部插入注射孔,缓慢注入细胞悬液40微升,轻轻旋转注射器90度,缓慢拔出针头,即可。 Extraction cell suspension syringe, the syringe needle is inserted into all the injection holes, 40 microliters of the cell suspension was slowly injected gently rotating the syringe by 90 degrees, slowly pull out the needle can.

[0021] 接种后动物常规饲养管理,每日观察动物的活动及采食情况,如果发现动物有死亡,及时解剖,观察记录直肠处是否有肿瘤生长。 [0021] conventional animal feeding and management, animal activity observed daily after inoculation and feed situation, if you find dead animals, promptly dissected, observed and recorded whether the rectum tumor growth. 观察时间为30天,观察期结束,颈椎脱臼处死动物,解剖观察成瘤情况及淋巴结转移情况,统计小鼠的肿瘤生成率及淋巴结转移率。 Observation time of 30 days, the end of the observation period, the animals were killed by cervical dislocation, Anatomy of the situation tumor and lymph node metastasis, tumor formation rate statistics mice and lymph node metastasis.

[0022] 结果,SW480细胞、Mel-CV细胞、HCT-116细胞各接种动物20只,均成瘤,成瘤率均为100%,各种肿瘤成瘤后均有淋巴转移现象,但淋巴转移率不同。 [0022] The results, of SW480 cells, Mel-CV cells, HCT-116 cells were seeded in each 20 animals, were tumor, tumor formation rate was 100% after a variety of tumors are tumor lymphatic metastasis phenomenon, lymph node metastasis different rates. 结果见下表。 The results in the table below.

[0023] 各种肿瘤细胞成瘤率统计表 [0023] The rate of tumor cells in various tumor Statistics

HcPue Ijo ko Ijx1e Ijo l10 ko HcPue Ijo ko Ijx1e Ijo l10 ko

2M-寸8〇~To IX1e To Too eo 2M- inch 8〇 ~ To IX1e To Too eo

細胭栋丨揉姊喜(縣站) 丨揉姊胭喜hrnw q\° I淋e转翁索 Kneading fine rouge Dong Shu percent Hi (s station) Shu kneading percent rouge hi hrnw q \ ° I e leaching cable turn Weng

Claims (3)

1.一种建立小鼠直肠移植瘤的装置,其特征在于:包括一个扩张固定管(I)和微量注射器(2),扩张固定管(I)前端面下部沿其长度方向凸出形成管头(3),扩张固定管(I)上部沿其长度方向开设有直线形注射孔(4),使用时微量注射器的针头插入注射孔(4),针头长度大于注射孔(4)长度,注射孔(4)的内侧端口位于管头上方的扩张固定管(I)前端面、夕卜侧端口位于扩张固定管(I)的外端面;扩张固定管(I)末端连接一个与扩张固定管具有折弯角的握持部(5),握持部(5)末端连接气囊(6),所述的握持部(5)、扩张固定管(I)和管头(3)内部开设有连通的气孔(7),气孔(7)末端连通气囊(6),气孔(7)前端出口开设于管头(3)顶面。 1. An apparatus for the rectum of mice xenografts established, characterized by: comprising a fixed pipe expansion (I) and a micro-syringe (2), the expansion of the fixed tube (I) formed in the front end of a lower surface of the ferrule projecting along the longitudinal direction thereof (3), expandable fixing tube (I) the upper portion defines a linear injection hole (4) along its length, the use of micro-needle of the syringe is inserted into an injection hole (4), the needle length is greater than the injection hole (4) the length of the injection hole (4) the inner side port is located on the front end face of the tube above the head of the expansion fixing tube (I), Xi Bu-side port is located dilation fixed pipe (I) an outer end surface; expandable fixing tube (I) terminus with one expanded fixed tube having off angled grip portion (5), the grip portion (5) connected to the end of the balloon (6), said grip portion (5), the expansion of the fixed pipe (I) and tube head (3) defines an internal communication hole (7), air holes (7) in communication terminal airbag (6), hole (7) opened in the front end of the outlet header pipe (3) top surface.
2.根据权利要求1所述的建立小鼠直肠移植瘤的装置,其特征在于:注射孔(4)的直径为0.45-0.5mm,长度为8-1 Ommn The means for establishing the rectum mouse xenografts according to claim 1, wherein: the diameter of the injection hole (4) is 0.45-0.5mm, a length of 8-1 Ommn
3.根据权利要求1所述的建立小鼠直肠移植瘤的装置,其特征在于:微量注射器针头直径为0.35-0.45mm,针头长度为14_16mm。 The means for establishing the rectum mouse xenografts according to claim 1, wherein: a micro syringe needle having a diameter of 0.35-0.45mm, the needle length 14_16mm.
CN 201410416546 2014-08-22 2014-08-22 Device for establishing rectal transplanted tumor in mice CN104173118A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201410416546 CN104173118A (en) 2014-08-22 2014-08-22 Device for establishing rectal transplanted tumor in mice

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201410416546 CN104173118A (en) 2014-08-22 2014-08-22 Device for establishing rectal transplanted tumor in mice

Publications (1)

Publication Number Publication Date
CN104173118A true CN104173118A (en) 2014-12-03

Family

ID=51954653

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201410416546 CN104173118A (en) 2014-08-22 2014-08-22 Device for establishing rectal transplanted tumor in mice

Country Status (1)

Country Link
CN (1) CN104173118A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105582600A (en) * 2015-12-16 2016-05-18 中国计量学院 Insect micro-injection method based on manual microsyringe

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5370675A (en) * 1992-08-12 1994-12-06 Vidamed, Inc. Medical probe device and method
WO2001012102A1 (en) * 1999-08-13 2001-02-22 Enteric Medical Technologies, Inc. Apparatus for forming implants in gastrointestinal tract and kit for use therewith
CN1429125A (en) * 2000-05-15 2003-07-09 阿雷斯贸易股份有限公司 Injection device
CN100496412C (en) * 2003-09-29 2009-06-10 伊西康内外科公司 Endoscopic mucosal resection device with outer tube and method of use
CN101869734A (en) * 2010-07-14 2010-10-27 哈尔滨工业大学 Negative pressure adsorption type hypodermic injection unit
CN201949479U (en) * 2011-01-30 2011-08-31 王有胜 Gynecological anesthetic injection device
CN102499784A (en) * 2011-10-24 2012-06-20 李云龙 Method and device for establishing bladder cancer in-situ model of nude mouse
US20120226300A1 (en) * 2007-07-11 2012-09-06 Vladimir Mitelberg Methods and Systems for Performing Submucosal Medical Procedures
US20120277698A1 (en) * 2007-04-30 2012-11-01 Andrew Mark S Harvesting fat tissue using tissue liquefaction
US20130041208A1 (en) * 2001-08-31 2013-02-14 Ams Research Corporation Surgical articles for placing an implant about a tubular tissue structure and methods
CN203525117U (en) * 2013-10-25 2014-04-09 江苏省人民医院 Support catheter

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5370675A (en) * 1992-08-12 1994-12-06 Vidamed, Inc. Medical probe device and method
WO2001012102A1 (en) * 1999-08-13 2001-02-22 Enteric Medical Technologies, Inc. Apparatus for forming implants in gastrointestinal tract and kit for use therewith
CN1429125A (en) * 2000-05-15 2003-07-09 阿雷斯贸易股份有限公司 Injection device
US20130041208A1 (en) * 2001-08-31 2013-02-14 Ams Research Corporation Surgical articles for placing an implant about a tubular tissue structure and methods
CN100496412C (en) * 2003-09-29 2009-06-10 伊西康内外科公司 Endoscopic mucosal resection device with outer tube and method of use
US20120277698A1 (en) * 2007-04-30 2012-11-01 Andrew Mark S Harvesting fat tissue using tissue liquefaction
US20120226300A1 (en) * 2007-07-11 2012-09-06 Vladimir Mitelberg Methods and Systems for Performing Submucosal Medical Procedures
CN101869734A (en) * 2010-07-14 2010-10-27 哈尔滨工业大学 Negative pressure adsorption type hypodermic injection unit
CN201949479U (en) * 2011-01-30 2011-08-31 王有胜 Gynecological anesthetic injection device
CN102499784A (en) * 2011-10-24 2012-06-20 李云龙 Method and device for establishing bladder cancer in-situ model of nude mouse
CN203525117U (en) * 2013-10-25 2014-04-09 江苏省人民医院 Support catheter

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105582600A (en) * 2015-12-16 2016-05-18 中国计量学院 Insect micro-injection method based on manual microsyringe
CN105582600B (en) * 2015-12-16 2019-04-05 中国计量大学 A kind of insect micro-injection method based on manual microinjector

Similar Documents

Publication Publication Date Title
CN1672649A (en) Cerebrospinal fluid puncturing drainer
CN101715322B (en) Sampling needle
CN102223854B (en) And access means for introducing and method comprising gametes, embryos, or any other type of fluid in the solution in the oviduct of a sow
CN2413708Y (en) Displsable pleuroperitoneal treating apparatus
US8956340B2 (en) Urethral catheter assembly with a guide wire
CN202313629U (en) Penetrative positioning pleural casing puncture needle
CN203539839U (en) Novel urethral catheter
CN202699713U (en) Visible anal tube for cleaning intestinal tract
CN2137939Y (en) Multipurpose expansible thoracic drainage trocar
CN202458288U (en) Endoscope casing
Stiles On the Dissemination of Cancer of the Breast, and the Necessity for its Treatment by Extensive Operation
CN2361288Y (en) Indwelling catheter assembly inside pleuroperitoneal cavity
CN202516058U (en) Mammary gland abscess double-laparoscope flushing drainage device
CN201127781Y (en) Perfusion needle head special for children
EP2833940B1 (en) Intra rectal/vaginal applicator technology
CN202289168U (en) Special urethra radiography catheter
CN203885900U (en) Drainage device used for thoracico-abdominal puncture
CN201930383U (en) Double-cavity drainage device after breast cancer operation
CN202060807U (en) Endometrial sampler
CN2829747Y (en) Medicine keeping enemator
CN2673428Y (en) Pig tail shaped lien drain pipe
CN204352256U (en) Sputum scab remover used in respiratory department
CN2536251Y (en) Disposable trocar puncture needle
CN201231004Y (en) Improved thorax lien needle for internal medicine
CN204995554U (en) Coaxial outer tube is assisted in lung aspiration biopsy

Legal Events

Date Code Title Description
C06 Publication
C10 Entry into substantive examination
RJ01