CN105572277B - The holding meanss of part for sample separation equipment, with varying strength - Google Patents
The holding meanss of part for sample separation equipment, with varying strength Download PDFInfo
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- CN105572277B CN105572277B CN201510719519.XA CN201510719519A CN105572277B CN 105572277 B CN105572277 B CN 105572277B CN 201510719519 A CN201510719519 A CN 201510719519A CN 105572277 B CN105572277 B CN 105572277B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6047—Construction of the column with supporting means; Holders
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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Abstract
The holding meanss (200) of part that the present invention relates to a kind of for sample separation equipment, with varying strength, the holding meanss (200) are for sample separation equipment (30) to be maintained in reversing chamber, the sample separation equipment (30) is for the fluid sample in separating sample separator (10), wherein, the holding meanss (200) include: fastening part (204), are designed to for the holding meanss (200) being fastened in the reversing chamber；Holding part (206) is connect with the fastening part (204), and the holding part (206) is designed to keep having the various sizes of sample separation equipment (30)；Wherein, the holding part (206) is designed to softer than the fastening part (204).
Description
Technical field
The present invention relates to a kind of holding meanss, back-fire arrangement, sample separator and sides for manufacturing holding meanss
Method.
Background technique
In HPLC, liquid (mobile phase) is usually with accurate controlled flow velocity (for example, at several microlitres to several milliliters per minute
In the range of) and (usually from 20 bars to 1000 bar or more, reach 2000 bars at present) under high pressure movement passes through stationary phase (example
Such as, chromatographic column), so that the independent element to the sample liquid being introduced into mobile phase separates, under high pressure, the pressure of liquid
Contracting is obvious.The HPLC system is in same Applicant Anjelen Sci. & Tech. Inc (Agilent
Technologies, Inc.) EP 0,309,596B1 in known to.
During separation process, column is placed at suitable temperature be necessary it is either desirable.For this purpose, will
Column is mounted in column oven and heats in column oven.Column is mounted in column oven usually uncomfortable for the user
, and often cause to separation process interference, non-uniform column heating.
Summary of the invention
It is an object of the invention to improve flexibility, handles sample separation equipment and acquisition is accurately separated result.The mesh
Solved by independent claims.Other embodiments are shown by dependent claims.
An exemplary embodiment of the present invention provides a kind of holding meanss, and the holding meanss are for separating sample
Equipment is maintained in reversing chamber, and sample separation equipment is for the fluid sample in separating sample separator, wherein holding meanss
Including fastening part, it is designed to for holding meanss being fastened in reversing chamber；Holding part is connect with fastening part,
Holding part is designed to keep having various sizes of sample separation equipment, wherein holding part is designed to than fastening
Part is soft.
According to another exemplary embodiment, a kind of tempering configuration is provided, which is configured to for separating sample
The sample separation equipment of fluid sample in this separator is tempered, wherein tempering configuration includes holding meanss and tempering
Room, holding meanss have above-mentioned feature and for keeping sample separation equipment, can be formed in reversing chamber and the external world
The different tempering temperature of temperature, and reversing chamber includes the mounting device for installing the fastening part of holding meanss.
According to another exemplary embodiment, a kind of sample separator is provided, the sample separator will be for that will flow
Fluid sample separation in dynamic phase is at fraction, wherein sample separator includes fluid delivery system, is used for transport flow phase
And fluid sample；Sample separation equipment, the fluid sample separation in mobile phase for being used to be conveyed by fluid delivery system at
Fraction；And holding meanss, as characterized above, the sample separation in the reversing chamber for remaining in sample separator
Equipment.
According to another exemplary embodiment, a kind of method for manufacturing holding meanss is provided, which uses
It being maintained in reversing chamber in by sample separation equipment, sample separation equipment is used for the fluid sample in separating sample separator,
Wherein, in this way, fastening part is designed for for holding meanss being fastened on reversing chamber, the guarantor connecting with fastening part
Hold part to design in this way: holding part is designed to keep various sizes of sample separation equipment, and keeps
Part is softer than fastening part.
Within the scope of application, particularly understand in this way term " soft ": softer holding part is harder
Fastening part mechanical strength it is smaller, therefore holding part is bigger than fastening part contractibility in the case where applied force.Tool
For body, holding part can surrender under application of a force and can change its shape, institute during being continuously applied power
The value of the power of application is in the range of the muscle strength of mankind user, however, during the influence of the power, the ruler of fastening part
It is very little substantially constant, that is, to keep its shape.Therefore, holding part can be designed as soft-component and fastening part can be set
It is calculated as hard component.Term " soft " refers specifically to holding part and the relatively low stability of fastening part.Stability is mechanical
Material property, it is characterised in that the ability of material resistance to deformation.Stability refers to the tension being calculated by maximum, force, this is most
It is obtained at defined strain relevant to the former cross-sectional area of material energetically.
Exemplary embodiment of the present invention have an advantage in that fastening part can by rigidly and size fixedly
It is fastened in reversing chamber, and therefore not easy to lose, however when holding part attaching sample separation equipment, with fastening part neighbour
The shape of the holding part connect is adapted to the shape of sample separation equipment, and however retentivity is applied to sample separation equipment
On, thus attaching sample separation equipment in a reliable fashion.The biddability and compliance of holding part allow holding part automatic
It is adapted to different size of sample separation equipment, and therefore holding part can be widely used in the sample separation to differ greatly
Experiment, the sample separating experiment have different requirements for the size of used sample separation equipment.By by means of protecting
It holds part and the be inserted into sample separator of fastening part holding is spaced, does not contact with the tempering wall of reversing chamber, it can be true
It protects fully or at least mainly by means of the heat exchange between sample separation equipment and ambient atmosphere rather than by means of tempering
The direct heat transfer of the heated wall of room executes tempering to sample separation equipment.Therefore, in the inside of sample separation equipment, it is ensured that right
Sample separation equipment is equably tempered, and without biggish temperature gradient, is avoided whereby by thermally-induced measurement illusion.
Due to the environment substantially to insulate caused by (especially by means of liquid chromatography), it can obtain and be accurately separated result.Tightly
Gu partially stabilized holding part makes it easy to operate and provides fastening function in reversing chamber.Holding part is allowed for soft
It is suitable for different size of sample separation equipment toughly, and especially provides foot by means of friction lock and/or clamping function
Enough high frictional force and/or chucking power, for steadilying maintain sample separation equipment.
In the following, holding meanss will be described, tempering configures, more embodiments of sample separator and method.
According to embodiment, holding part can be formed by the material softer than fastening part.As a result, according to this embodiment, protecting
Holding part and fastening part can be made of different materials, so that for their own function, two parts can be with
The special mode of material technology adapts to.Specifically, on the one hand the material with different mechanical strengths can be used for holding part, and
And it on the other hand can be used for fastening part.
According to embodiment, holding part is at least formed in some parts by material identical with fastening part, and at least
It is lower than the strength of materials of fastening part in some positions.Therefore, specifically, the embodiment of a part of holding meanss is also can
With, wherein different machinery can be obtained by the different strengths of materials (specifically, material thickness and/or material shape)
The fastening part and holding part of intensity.The characteristic that the lower strength of materials causes the relatively high strength of materials softer, and it is anti-
?.It is specified along entire fastening part and along entire holding part or only in certain parts or at certain positions
These different strengths of materials.The lower strength of materials at certain positions can be enough to generate elasticity or mechanically deform (example
Such as, relatively thin part, specifically, it is at holding part as reversible predetermined kinking point).
According to embodiment, holding part can be formed by elastic material and fastening part can be formed by rigid material.
Elastic material can be understood as such material (specifically, opposite with plastic deformable material): by making specific material
Zone bias in its remainder unstressed position and generate the reset force proportional to deflection size, to show ideal
The characteristic of spring.On the contrary, rigid material can be understood as such material: when user is applied through operation sample separation equipment
Generated muscle strength will not realize functionally apparent deformation.
According to alternate embodiment, holding part and fastening part can be formed as component that is separating and being connected to each other,
The component being connected to each other with especially forming locking ground and/or friction lock.According to this embodiment, firstly, holding part and tight
Gu part can by so that they specific function optimize in a manner of it is independent of each other manufacture and be then connected to each other.It is specific and
Speech, the embodiment are allowed for selecting different materials for holding part and fastening part.It can be only for example, by clogging mutually
Holding part and fastening part are attached, to form bracket connection.
According to embodiment, fastening part and/or holding part may include plastics.Alternatively, fastening part and/or maintaining part
Dividing can be manufactured by other materials, for example, ceramics or metal.
According to embodiment, fastening part can by by polyamide, glass filled polyamide, acronitrile-butadiene-styrene,
Material in the group of polyethylene and polytetrafluoroethylene (PTFE) composition is formed.These materials are reliably tight by holding meanss with being enough to ensure that
Gu the intensity in reversing chamber.In addition, these materials have sufficiently low pyroconductivity, to prevent from being tempered by fastening part
It is conducted heat in large quantities between room and sample separation equipment.Similarly, these materials are adapted so that manufacture is tight in cost-effective manner
Gu part.
According to embodiment, holding part can be by by thermoplastic elastomer (TPE), rubber, silicone, ethylene propylene diene rubber and poly- two
Material in the group of first siloxanes composition is formed.These materials include enough mechanically deforms for making the outer of holding part
Shape is adapted to the sample separation equipment of the size just used in a reliable fashion.Meanwhile these materials have sufficiently low heat
Conductivity, to prevent from being conducted heat in large quantities between reversing chamber and sample separation equipment by holding part.In addition, these materials
Material is adapted so that manufactures holding part in cost-effective manner.
According to embodiment, fastening part can be designed for tool-lessly, particularly manually fastening holding meanss
In reversing chamber.Since fastening part is installed in reversing chamber or is dismantled from reversing chamber fastening part without tool by user
Point, it is possible to obtain higher users'comfort.For example, user only by fastening part be arranged in reversing chamber and then
Rotational fastener part can be enough for holding meanss to be securely fastened in reversing chamber.
According to an embodiment of the invention, fastening part can be designed for being reversibly fastened in reversing chamber.In other words,
Fastening part can engage in reversing chamber (for example, at different positions) to be removed at this several times and again.In addition,
Holding meanss can be used in several different reversing chambers.It is flexibly and universal that the reversible availability of holding meanss is applicable in it.
According to embodiment, when fastening part is completely tightened in reversing chamber, when being especially latched in reversing chamber, fastening part
It is designed to provide for the tactile for indicating to fasten completely and/or sound feedback.In other words, when holding meanss successfully fasten completely
When in reversing chamber, holding meanss are provided to user and are safely attached to the apparent touch feedback in reversing chamber at this time.Cause
This, improves the comfort level of user and at the same time providing and the fastening force in order to avoid too big by user's application is effectively protected,
When there is the power, holding meanss and/or reversing chamber may be damaged.It can be audibly (for example, using that can hear
Ticktack) it prompts to complete fastener cycle to user.
According to embodiment, fastening part may include rotating closed device, which is used in reversing chamber
Rotational fastener at relative rotation closing device.It is characterized in that being attached to holding meanss on reversing chamber and then by holding meanss
Being screwed to the fastener cycle at fastening position for the user is simple and easy and can intuitively know.Meanwhile
The rotational fastener prevents holding meanss to be undesirably removed from each pull and to improve the stability of fastening.It is alternative
Ground or additionally, fastening part can be clamped in the mounting device of reversing chamber.
According to embodiment, rotating closed device includes protruding portion, which has what is changed in the circumferential to radially extend,
Rotating closed device can be fastened in a manner of shape lock (form-locking) and/or friction lock rotation driving
In the recess of the relative rotation closing device of reversing chamber.If forming what outer diameter circumferentially changed in the fastening area of fastening part
Cyclic lug, then by the way that the circumferential section with small radii is placed in indent and will then have larger radius
Another circumferential section is screwed in recess, it will be able to which holding meanss are fastened in reversing chamber by realization.By using simple
Mechanical device, simple fastener cycle are combined with the fastening of high reliability.
According to embodiment, holding part can be designed for keeping diameter at least in 3mm to the sample separation between 12mm
Equipment.Specifically, splitter usually has the size, and then according to for chromatographic isolation experiment
Embodiment keeps these columns using the same commonly available holding meanss.Due to the sufficiently soft of holding meanss, surrender and bullet
Property design, the materials of holding meanss tightens various sizes of sample separation equipment, and by combining elastic reset power and rubbing
Wipe power keeps this to tighten between holding part and sample separation equipment, specifically, can be special using rubber holding part
Effectively applied force.
According to embodiment, fastening part may include base portion and bearing part, wherein base designs are for being fastened on tempering
In room and holding part is arranged in bearing part.Base portion and bearing part can be positioned relatively to each other, and ensure sample
There are spacing between this separation equipment and reversing chamber.This promotes temperature to be uniformly distributed along the entire extension of sample separation equipment.
According to embodiment, holding part angularly, especially with the angle between 30 ° to 150 °, more particularly with 60 °
Angle between to 120 ° is arranged in bearing part.For example, carrying of the holding part from the carrying bearing part of holding part
Surface extends vertically.Specifically, the outer end for avoiding reversing chamber of holding part and bearing part are angled.To for example exist
Cavity is formed before holding part, the cavity is in beak and the cavity is towards bearing part formation taper, which promotes
Intuitively and easily the sample separation equipment with different-diameter is inserted into holding part.
According to embodiment, holding part includes multiple elastic holding structures for keeping sample separation equipment.For example, this
A little holding structures may include thin slice (lamella) (the especially thin slice with different longitudinal extensions and/or thickness), brush
(the especially brush with different longitudinal extensions and/or thickness), burling (burling) (especially have different longitudinal directions
Extend and/or thickness it is burling), tooth (especially with it is different it is longitudinal extend and/or the tooth of thickness) and/or at least hole knot
Structure (an especially at least sponge).It is multiple in wherein these holding meanss with cavity by providing, it can further mention
Adaptability of the high holding meanss to various sizes of sample separation equipment.
According to embodiment, structure is kept angularly, especially with the angle between 30 ° to 150 °, more particularly with 60 °
Angle between to 120 ° extends from bearing part.For example, keeping structure that can stretch vertically from bearing part.It is keeping tying
Structure in the case where inclination arrangement, can be formed for user for being inserted into respective sample separation equipment since bearing part
The visible slot being intuitively inserted into.
According to embodiment, holding meanss can be formed as keeping various sizes of based on chucking power and/or frictional force
Sample separation equipment.Chucking power and frictional force are allowed for reversibly installing and dismantle various sizes of sample separator simultaneously
And simultaneously for reliably keeping.
According to embodiment, holding part and/or fastening part by pyroconductivity difference material or heat insulator, especially
Material with the pyroconductivity less than 1W/mK is formed.Specifically, holding meanss and reversing chamber can shapes in this way
At: if holding meanss keep sample separation equipment and holding meanss to be fastened in reversing chamber so that sample separation equipment and
It can not be contacted between reversing chamber and/or directly heat transfer connects.According to these preferred embodiments, it is maintained in holding meanss
Sample separation equipment can by means of for thermal conductivity at least in the holding meanss of certain part thermal insulations or coefficient of overall heat transmission difference it is big
It causes to be pyrolyzed coupling with the heated wall of reversing chamber.Sample separation equipment is returned using reversing chamber (it for example may include heated wall)
Fire is mainly realized by the atmosphere in reversing chamber, part of it can be tempered and (especially be heated) by reversing chamber.
Thereby it is ensured that sample separator equably and is spatially equably tempered, this improves the precision of separating resulting.
According to embodiment, holding part and fastening part by temperature at least to 110 °, especially at least to 130 ° when still
Stable material is formed.Therefore, holding meanss can resist the prevailing temperature in the column oven of modern liquid chromatography device.
According to embodiment, tempering configuration can include at least the Further retention devices with the above-mentioned feature referred to, wherein
It will by means of holding meanss and at least one Further retention devices (especially by means of two identical holding meanss of construction)
Sample separation equipment is maintained at different positions.Specifically, can be relative to each other at it by means of respective holding meanss
Two ends region in keep substantially tubular and cylindrical sample separation equipment so that in holding meanss and sample
Applied force on this separation equipment is sufficiently low and is spatially evenly distributed.
According to embodiment, reversing chamber may include temperable tempering wall, and at tempering wall, assembling device is formed for pacifying
Fastening part is filled, recess is uniquely formed as, is more particularly formed as V-arrangement recess, T shape recess or semi-circular indentation, it is especially special
Not there is external wedge.It is particularly advantageous that at least one guide rail describe receive recess can be set tempering wall on, the recess
It is formed for and is suitable for generally and at an arbitrary position place one or more holding meanss (and/or other portions are installed
Part, for example, the preheating apparatus of the fluid of the upstream for preheating sample separation equipment).For example, along the longitudinal direction for accommodating recess
Extending direction two holding meanss separated from each other can be configured to accommodate two different regions of sample separation equipment
(that is, end regions) can be configured to accommodate two different sample separation equipments (it can also have different sizes)
And/or it can be configured to accommodate sample separation equipment and other fluidic components (for example, for heating sample separation equipment
The preheating apparatus of trip).It is, therefore, possible to provide be defined by the user, accommodate it is several similar and/or different fluidic component
Modular system especially provides these in reversing chamber and accommodates several in recess.
Verified V-arrangement receiving recess has been combined with external wedge is particularly advantageous.Such as it should shown in Figure 14
Construction allows first fluid component (for example, preheating apparatus) to be contained in V-arrangement receiving recess, at the heating surface of tempering wall
Directly (especially can heat transfer) thermal coupling, and allow simultaneously to accommodate second fluid component (for example, sample separation equipment)
At external wedge, there was only advantageous (by means of the gas transport in reversing chamber) thermal coupling indirectly at the heating surface of tempering wall
It closes.Path is shorter due to fluidly connecting between these fluidic components, this saves the layout of the fluidic component in reversing chamber
Space.Setting V-arrangement accommodates recess and additionally provides such advantage: when sample separation equipment is undesirably inserted directly by user
To V-arrangement accommodate recess in when (this can undesirably make sample separation equipment at the position contacted with reversing chamber directly and because
This is unevenly heated), sample separation equipment can skid off V-arrangement under gravity and accommodate recess and to prevent user
Sample separation equipment is operated in a manner of inappropriate.Therefore, parasitic directly heat is impossible.
Alternatively, holding meanss can be (reversibly or irreversibly) to be bonded in reversing chamber.
According to embodiment, holding part can especially be molded at least part of double teeming fastening part by means of double teeming
It is formed.For example, firstly, manufacturing fastening part according to the first injection molding process.Then, the fastening part manufactured in this way can
To be inserted into another injection molding room and be used to form the material injection molding of holding part.This ensures maintaining part
Divide the close connection between fastening part, and however the material alone of holding part and fastening part is allowed to be suitable for protecting
Hold the different functions of part and fastening part.
According to another embodiment, holding part and fastening part can manufacture with being separated from each other, especially respectively by
Manufacture with being separated from each other, and can then be connected to each other in injection molding, especially friction lock and/or shape lock each other
Connection.For example, holding part can be manufactured in the first injection molding room.Furthermore, it is possible to be manufactured in the second injection molding room
Fastening part.The part manufactured in this way can then be assembled into holding meanss.This can be for example by forming mechanical detent
Or other connections, or purely mechanically manufactured also by using bonding or by means of joint method (for example, welding).
According to embodiment, sample separator can be structured as micro-fluid measurement device, liquid-chromatography apparatus or HPLC.
It is (super that sample separator especially can be structured as HPLC device (high performance liquid chromatography), life science apparatus or SFC device
Critical fluids chromatography).Certainly, other applications are also possible.
According to embodiment, sample separator can be structured as pressure resistance with for operating in the case where reaching about 100 bars of pressure,
It is particularly used for operating in the case where reaching about 500 bars of pressure, more specifically to be operated in the case where reaching about 1000 bars of pressure.
According to embodiment, the sample separator as sample separation equipment may include splitter, which is used for
Separate the different fractions of injected fluid sample.The splitter can be filled with absorbing medium, for example, by silica gel or active carbon
Manufactured porous bead.By the chemical reaction with the porous bead, fluid sample can be in splitter by temporary fixing or inhaled
It is attached.For example, passing through the gradient of setting solvent ingredient, independent fraction can independently be dissolved and be released from adsorbing medium and inhale
It is attached, and can then be detected.
According to embodiment, fluid sample and mobile phase of the analysis pump designed for conveying injection.Mobile phase can be molten
Agent ingredient, the solvent composition can be temporarily constant or be changed in a manner of adjustable, and will be flowed by means of sampling valve
After body sample is injected into sample disjoint paths, which will mix with fluid sample.Mobile phase and fluid sample
Mixture will pass through chromatographic isolation path by means of being formed as the analysis pumping of high-pressure pump.Sample separator can also wrap
One or more pumps are included, one or more pump is separated for conveying the fluid sample of injection together with mobile phase by sample
At least part of device.The pump can be configured to mobile phase for example under high pressure (for example, a few hectobars are to 1000 bars or more)
Pumping passes through system.
According to embodiment, sample separator may include the sample for detecting the sample component of separated fluid sample
This detector.The sample detector can be based on detection electromagnetic radiation (example as caused by the predetermined fluid ingredient of fluid sample
Such as, in the field of UV range or visible range) testing principle.
Alternatively or additionally, measuring device may include the sample point for being fractionated to separated sample component
Evaporate device (fractionator).Different sample components can be for example transported in different containers by the fractionator.It is analyzed
Fluid sample can also be supplied to waste canister.
Detailed description of the invention
By reference to the detailed description below in conjunction with attached drawing to embodiment, it can more easily recognize and more fully understand
Other purposes and many added benefits of the embodiment of the present invention.Substantially functionally identical or similar feature will be with equally
Appended drawing reference indicate.
Fig. 1 shows the HPLC- measuring device of an exemplary embodiment of the present invention.
Fig. 2 and Fig. 3 shows the two spaces view of the holding meanss of an exemplary embodiment of the present invention.
Fig. 4 shows the fastening part of the holding meanss of an exemplary embodiment of the present invention.
Fig. 5 and Fig. 6 shows the tempering configuration of an exemplary embodiment of the present invention.
Fig. 7 to Figure 12 shows the holding meanss of an exemplary embodiment of the present invention.
The side of tempering configuration that Figure 13 shows an exemplary embodiment of the present invention, installing sample separation equipment thereon
View.
Figure 14 shows the tempering wall and mounting device of the tempering configuration of an exemplary embodiment of the present invention.
Figure 15 shows the detail view of a part of the reversing chamber according to Figure 14.
Figure 16 and Figure 17 shows the tempering configuration of an exemplary embodiment of the present invention.
Diagram in attached drawing is exemplary.
Specific embodiment
Before describing exemplary embodiment of the present invention with reference to the accompanying drawings, some basic problems are proposed, it is basic based on these
Exemplary embodiment of the present invention is unfolded in problem.
Accoding to exemplary embodiment, a kind of column retainer is proposed, is mounted on chromatographic column as clamping and grip device
The inside of incubator, for keeping the column of different-diameter in a reliable fashion.After installation, holding meanss rest on making for column oven
At position defined by user, and chromatographic column mounted thereto is held in place.After user's installation, it can prevent
Undesirable movement occurs for holding meanss and column.It is very simple by the inside that holding meanss are mounted on column oven and is not necessarily to
Tool.Holding meanss for example may be mounted in wedge (dove tail) recess of column oven, move along these recesses, and
And therefore it is suitable for the column of different length together with other holding meanss.Due to the flexible construction of holding meanss, can fit
Answer different column diameters.Since holding meanss according to the embodiment are monolithic design, user can prevent in a reliable fashion
Complicated multi-part device loses small part.Instead of wedge recess, dress can be kept to accommodate in reversing chamber using undercutting
It sets, which is that T shape or triangle or (partly) are round.In addition, can be protruded on the wall (that is, planar wall) of reversing chamber
Place mounting device in ground.Twist lock mechanism has can be set to allow rotational fastener in holding meanss.If the holding meanss are arranged in
It in recess and is rotated, holding meanss will be fixed in recess.This allows holding meanss along recess free positioning.However,
If holding meanss are mounted in recess, holding meanss will be fixed on recess in a manner of loading for shifting and sliding
In, until user discharges spin locking and other fixations and in new ways along receiving recess positioning holding device.This can be with
Implement several times without using tool.After holding meanss to be installed to receiving recess, simply column can be inserted into
In the holding part for receiving notch or other constructions at the external available position of holding meanss, wherein can also not use
The execution of instrument process.Implement the disassembly of column by taking out column from holding part.Due to holding meanss front side it is soft
Property, all columns with different-diameter can be held in place in a reliable fashion, so that being used for color without different model
Spectrum analysis purpose holding meanss.The holding meanss of design can accommodate different columns.By using the spring material of flexible metal,
Holding part can be made to obtain desired flexible and elasticity by using rubber type of material etc..Suitable forming will be (for example, will keep
Structure is designed as thin slice, tup, brush, blade, ball etc.) it can aid in and (suitably for example gather for the material of holding part
Close object) flexibility.The holding meanss reversibly can be bent and/or fasten in this way and to can be adapted for different columns straight
Diameter, wherein column can be prevented undesirably under attachment state while being moved.Holding meanss are preferably formed by heat insulator, with
Just the adiabatic temperature control to column is not offset.
Fig. 1 is shown as the theory structure of the exemplary HPLC- system for sample separator 10, sample separation dress
It sets and for example can be used for liquid chromatography.20 driving mobile phases of analysis pump by the separator as sample separation equipment 30, by
Dissolution vessel 25 provides mobile phase and passes through the gas in 27 exhaust flow phase of deaerator, comprising fixing in sample separation equipment
Phase.Sample input unit (also referred to as sample injector or injector) 40 be arranged in pump 20 and sample separation equipment 30 between, with
It will be flowed in the fluid switch (it can count (counted to) injector 40) by means of an exemplary embodiment of the present invention
Body sample is injected into mobile phase.The stationary phase of sample separation equipment 30 is intended to the sample component of separating sample liquid.Detector
The sample component of the 50 separated samples of detection, and fractionating device 60 can be used for by the sample of separated sample liquid at
It point is for example put into the container provided for it or outlet.The component of the control sample separator 10 of control device 70.
Although the liquid path between pump 20 and sample separation equipment 30 is generally under high pressure, sample liquid is initial
It is input into the region separated with liquid path under normal pressure, which is the sample loop of so-called injector 40 (as sample
This receiving volume), transfer to be input to sample liquid in liquid path under high pressure.When will be initial in sample loop
When sample liquid under normal pressure is linked into the liquid path under high pressure, the content of sample loop is suddenly (usually in millisecond
In the range of) reach pressure in the system of sample separator 10.
In Fig. 1, still further it can be seen that sample separation equipment 30 is arranged in reversing chamber 202 simultaneously together with preheating apparatus 90
And it can be heated in reversing chamber by heating source 80.More particularly, heating source 80 heats the wall of reversing chamber 202.Preheating
Device 90 be directly arranged on the wall of reversing chamber 202 and to directly with wall thermal coupling, fluid be flowed into sample separation set
Preheating apparatus 90 is flowed through before standby 30.With preheating apparatus on the contrary, 30 thermal insulation of sample separation equipment be arranged in reversing chamber
On 202 wall, and therefore the elevated temperature gas heated by the heated wall in reversing chamber 202 add indirectly and heat-insulatedly
Heat.
Fig. 2 and Fig. 3 shows the different views of the holding meanss 200 of an exemplary embodiment of the present invention, can pass through
Sample separation equipment 30 is installed in reversing chamber 202 by the holding meanss 200.
Holding meanss 200 are used to for sample separation equipment 30 being maintained at the reversing chamber of the sample separator 10 according to Fig. 1
In 202.Reversing chamber 202 includes gas chamber 270 and temperable tempering wall 210, and on tempering wall, mounting device 208 is outer to have
The form of the V-arrangement recess of portion's wedge is formed, and external wedge is used to install the fastening part 204 of holding meanss 200.
Holding meanss 200 are also formed by fastening part 204 and holding part 206, and fastening part is formed for fastening tempering
Holding meanss 200 in room 202, and holding part is connect with fastening part 204, and which forms for keeping different
The sample separation equipment 30 of size (especially with different-diameter), wherein only keeping a specific sample at every point of time
Separation equipment 30.Holding part 206 is softer than fastening part 204 (that is, having lower stability).Therefore, elastic holding part
206 obtain the shape for being suitable for accommodating various sizes of sample separation equipment 30, pass through folder in a reliable fashion without losing
Holding force and/or frictional force fix the ability of the sample separation equipment 30.It is with elastic holding part on the contrary, relatively rigid (that is, having
Higher stability) fastening part 204 formed in this way: even if it still maintains size under the influence of fastening force
Stablize and therefore prevents holding meanss 200 undesirably from the installation dress at reversing chamber 202 and/or in reversing chamber 202
It sets in 208 and drops out.
Holding part 206 is for example formed silicone resin and therefore ratio such as fastening part 204 made of polyamide
Softer material is formed, and holding part is also known as soft-component, which is referred to alternatively as hard component.The material selection mentioned
Also cause between sample separation equipment 30 and reversing chamber 202 good heat-insulated, sample separation equipment is maintained at holding part 206
In, holding meanss 200 are arranged at reversing chamber by means of fastening part 204.It is accordingly possible to ensure sample separation equipment 30 is advantageous
The environment temperature heating that ground is only heated properly, so that sample separation equipment 30 is under the conditions of being insulated and therefore boundary is specific
Work.In addition, these materials are suitable for resisting the heating temperature (example present in the reversing chamber 202 tested for chromatographic isolation
Such as, reach 110 DEG C).In addition, as shown in figure 3, it is lower that holding part 206 is formed in the strength of materials compared with fastening part 204
Region in.In addition, the component that holding part 206 and fastening part 204 discretely and mutually form a fixed connection.
As shown in Figures 2 and 3, holding part 206 includes continuous notch 234, is installed to reversing chamber in holding meanss 200
In the state of 202, which extends along the corresponding receiving recess 236 of mounting device 208, in the mounted state sample point
Extend from equipment 30 also along the corresponding recess 236 that accommodates.When sample separation equipment 30 is inserted into holding part 206,
Notch 234 expands, to allow to accommodate sample separation equipment 30.In addition, the elastic material of holding part 206 is in notch 234
Apply the power of clamping fastener and friction fastening sample separation equipment 30 at two sides.Holding part 206 includes for keeping sample point
Multiple elastic holding structures 300 from equipment 30, in an illustrated embodiment, elastic holding structure is at the two sides of notch 234
It is formed to have the thin slice of free end being arranged symmetrically.These keep structure 300 that can be set according to the sample separation respectively accommodated
Standby 30 size substantially or slightly is unfolded or is more specifically resiliently bent, and the holding structure is in wider coupling part
Unilateral connection in groups mutually at 302.Design with the holding part 206, holding part 206 are suitable for keeping diameter
Sample separation equipment 30 between 3mm to 12mm.According to the size of the sample separation equipment 30 of holding, substantially or
Person small size ground enlargement indentation 234.Artificially and without tool by individual sample separation equipment 30 it can be inserted into maintaining part
Divide in 206, and then again artificially and without tool takes individual sample separator from holding part 206
Out.
Particularly before sample separation equipment 30 is inserted into holding part 206, make fastening part 204 with manually and
Reversibly mode (that is, can discharge and re-secure again) is formed at the mounting device 208 of reversing chamber 202, fastening part
For tightness retaining mechanism 200 without tool.This can be realized by means of rotational shell mechanism, will be carried out with reference to Fig. 4 to it
It is more fully described.Fastening part 204 has reversing chamber side group portion 220 and sample separation equipment side bearing part 222.Base portion
220 are formed at the mounting device 208 of reversing chamber 202 for fastening.Holding part 206 is arranged at bearing part 222 simultaneously
And patchery (patch) is wherein.It is preferred that fastening part 204 is formed as when being completely tightened in reversing chamber 202 (that is, being locked in
When at reversing chamber 202) tactile (that is, shock can be felt at end position) sound for fastening completely of instruction is provided (that is, energy
Enough hear shock) feedback.Holding part 206 extends vertically from bearing part 222.In addition, keeping structure 300 from bearing part
Extend vertically at 222.
Fig. 4 shows the holding part of the holding meanss 200 of an exemplary embodiment of the present invention and specifically illustrates base
The embodiment in portion 220.According to Fig. 4, fastening part 204 includes rotating closed device 400, which can be used for device in Fig. 2
In visible, reversing chamber 202 the mounting device 208 for forming wedge recess relative rotation closing device to form rotation tight
Gu.Rotating closed device 400 includes protruding portion, and protruding portion has radical length r, R (wherein, r < different in a circumferential direction
R), the phase that the protruding portion is fastened on reversing chamber 202 by revolving actuator in a manner of shape lock and/or friction lock can be used
To the indent of rotating closed device.Initially in such a way that the region with small radii r is disposed with enough tolerances, it will keep
Device 200 is inserted into the relative rotation closing device to form the mounting device 208 of wedge recess, and replaceable to be suitable for
Ground is arranged in wedge recess.It, can be along Fig. 4 after selecting desired installation location along wedge recess for holding meanss 200
Middle label has 402 direction of rotation rotation holding meanss 200, until the region with longer radius R enters point
It splits in recess, and therefore causes in reversing chamber 202 based on chucking power and based on frictional force tightness retaining mechanism 200.
Fig. 5 and Fig. 6 shows the tempering configuration 500 of an exemplary embodiment of the present invention.
In sample separator, shown in tempering configuration 500 for being tempered to sample separation equipment 30, sample point
From device for the fluid sample in separating sample separator 10.Tempering configuration 500 respectively includes several holding meanss 200,
Several holding meanss are used for several, the various sizes of sample separation equipments 30 remained mounted in reversing chamber 202.In reversing chamber
In 202, the tempering temperature of ambient temperature can be risen above by heating device (not showing in fig. 5 and fig.).Respective time
Fiery room 202 includes respective mounting device 208, with the fastening part 204 for installing holding meanss 200.
Various sizes of sample separation equipment 30 shown in fig. 5 is distinguished by means of two independent dedicated holding meanss 200
It is maintained at independent two different positions.
Fig. 6 shows the different holding meanss 200 with different holding structures 300, that is, one has thin slice, a tool
There is teeth portion, one has relative to each other and matched two clamping jaws, and one has flat spring structure.
Fig. 7 to Figure 12 shows the holding meanss 200 of an exemplary embodiment of the present invention.Holding dress is shown in FIG. 7
Set 200, the fastening part 204 of holding meanss is rigid and substantial rectangular shaped and holding meanss in a top view
Holding part 206 include two clamping jaws relative to each other.Had according to the holding meanss 200 of Fig. 8 and is filled with according to the holding of Fig. 7
Set 200 similar holding parts 206, however it is tight according to the fastening part 206 that the holding meanss of Fig. 8 include relative to each other two
Clamped support member has the free end 800 widened.Holding meanss 200 shown in Fig. 9 are different from shown in Fig. 3 in this way
Holding meanss according to Fig. 3 there are the slice-shaped holding meanss of free end not connected at interconnecting piece 302 in groups merely on outer side according to Fig. 9
It connects but also the annular closed in sample separation equipment-housing region.According to the holding meanss 200 of Figure 10 substantially with Fig. 3's
Holding meanss are corresponding.According to the holding meanss 200 of Figure 11 in the side for being supplied to sample separation equipment 30 of holding meanss 200
It is upper that there are additional recesses 1100.The 300 thin slice convex of holding structure that holding meanss 200 according to Fig. 9 to Figure 11 include, and root
Keep structure 300 recessed outward according to the thin slice in the holding meanss 200 of Figure 12.
Tempering configuration that Figure 13 shows an exemplary embodiment of the present invention, being equipped with sample separation equipment 30 thereon
500 side view.Accommodating recess 236 includes accommodating chamber 1300 and tool substantially v-shaped, for accommodating preheating apparatus 90
External accommodating chamber 1302 that have side wedge recess, that holding meanss 200 can be accommodated.Figure 13, which is also shown, accommodates recess 236 with this
The mode of sample is formed: preheating apparatus 90 is contained in V-arrangement accommodating chamber 1300 and at the same time holding meanss 200 are contained in external appearance
It receives in chamber 1302.
Figure 14 shows the reversing chamber 202 of the tempering configuration 500 of an exemplary embodiment of the present invention.In reversing chamber 202
In, the receiving recess 236 parallel to each other of several rail shapes is provided, allows to install multiple fluidic components.Figure 15 is shown
According to the detail view of a part of the reversing chamber 202 of Figure 14.
Figure 16 and Figure 17 shows the tempering configuration 500 of an exemplary embodiment of the present invention.
It should be noted that term " includes " is not excluded for other elements and the article " one " is not excluded for multiple situations.In addition, in conjunction with
Different embodiments and the element described can be combined.It should also be noted that the appended drawing reference in claim should not be construed as limitation power
The range that benefit requires.
Claims (44)
1. a kind of holding meanss (200), the holding meanss (200) are used to sample separation equipment (30) being maintained at reversing chamber
(202) in, the sample separation equipment (30) is for the fluid sample in separating sample separator (10), wherein the guarantor
Holding device (200) includes:
Fastening part (204) is designed to for the holding meanss (200) being fastened in the reversing chamber (202)；
Holding part (206) is connect with the fastening part (204), and the holding part (206) is designed to keep tool
There is the various sizes of sample separation equipment (30)；
Wherein, the holding part (206) is designed to softer than the fastening part (204),
Wherein, the holding part (206) includes multiple elastic holding structures for keeping the sample separation equipment (30)
(300),
Wherein, the holding structure is selected from by the group of thin slice, brush, fritter, tooth or at least one pore structure composition.
2. holding meanss (200) according to claim 1, wherein the thin slice is different longitudinal extension and/or thickness
Thin slice.
3. holding meanss (200) according to claim 1, wherein the pore structure is sponge.
4. holding meanss (200) according to claim 1, including one of following characteristics:
Wherein, the holding part (206) is formed by the material softer than the fastening part (204)；
Wherein, the holding part (206) is formed by material identical with the fastening part (204), and the maintaining part
Divide the strength of materials of (206) at least at some positions than the fastening part (204) low.
5. holding meanss (200) according to claim 1, wherein the holding part (206) is formed by elastic material,
And the fastening part (204) is formed by rigid material.
6. holding meanss (200) as claimed in any of claims 1 to 5, including one of following characteristics:
Wherein, the holding part (206) and the fastening part (204) are formed as a component；
Wherein, the holding part (206) and the fastening part (204) are formed as component that is separating and being connected to each other.
7. holding meanss (200) according to claim 6, wherein the holding part (206) and the fastening part
(204) it is bonded to one another to be formed.
8. holding meanss (200) according to claim 6, wherein the holding part (206) and the fastening part
(204) component being connected to each other with being formed as shape lock and/or friction lock.
9. holding meanss (200) as claimed in any of claims 1 to 5, wherein the fastening part (204) by
The material in group be made of polyamide, glass filled polyamide, acronitrile-butadiene-styrene, polyethylene and polytetrafluoroethylene (PTFE)
It is formed.
10. holding meanss (200) as claimed in any of claims 1 to 5, wherein the holding part (206) by
The material in group being made of thermoplastic elastomer (TPE), rubber, silicone, ethylene propylene diene rubber and dimethione is formed.
11. holding meanss (200) as claimed in any of claims 1 to 5, wherein fastening part (204) packet
Rotating closed device (400) are included, to be used for rotational fastener at the relative rotation closing device (208) of the reversing chamber (202).
12. holding meanss (200) according to claim 11, wherein the rotating closed device (400) is included in circumferential direction
Upper to have the different protruding portions for radially extending (r, R), the protruding portion with shape lock and/or rubs under the action of rotating driving
The mode for wiping locking is fastened in the recess of the relative rotation closing device (208) of the reversing chamber (202).
13. holding meanss (200) as claimed in any of claims 1 to 5, wherein fastening part (204) packet
Including base portion (220) and bearing part (222), wherein the base portion (220) is designed for being fastened in the reversing chamber (202),
And the holding part (206) is arranged substantially at the bearing part (222).
14. holding meanss (200) according to claim 13, wherein the holding part (206) is angularly arranged in
At the bearing part (222).
15. holding meanss (200) according to claim 14, wherein the holding part (206) with 30 ° to 150 ° it
Between angle be arranged at the bearing part (222).
16. holding meanss (200) according to claim 14, wherein the holding part (206) with 60 ° to 120 ° it
Between angle be arranged at the bearing part (222).
17. holding meanss (200) according to claim 1, wherein the holding structure is from the bearing part (222)
Angularly extend.
18. holding meanss (200) according to claim 17, wherein the holding structure is from the bearing part (222)
Extended with the angle between 30 ° to 150 °.
19. holding meanss (200) according to claim 17, wherein the holding structure is from the bearing part (222)
Extended with the angle between 60 ° to 120 °.
20. holding meanss (200) as claimed in any of claims 1 to 5, including at least one in following features
Person:
Wherein, the fastening part (204) is designed for being tool-lessly fastened to the tempering for the holding meanss (200)
In room (202)；
Wherein, the fastening part (204) is designed for being reversibly fastened in the reversing chamber (202)；
Wherein, the fastening part (204) is designed for when being completely tightened in the reversing chamber (202), provides and has indicated
The tactile and/or sound feedback fastened entirely；
Wherein, the holding part (206) is designed for keeping the diameter sample at least between 3mm to 12mm point
From equipment (30)；
Wherein, the holding part (206) is designed for keeping various sizes of in a manner of based on chucking power and/or frictional force
Sample separation equipment (30)；
Wherein, the holding part (206) is formed by heat-barrier material；
Wherein, it is still stable when the holding part (206) and the fastening part (204) are at least up to 110 DEG C by temperature
Material is formed.
21. holding meanss (200) according to claim 20, wherein the fastening part (204) is designed for manually
The holding meanss (200) are fastened in the reversing chamber (202).
22. holding meanss (200) according to claim 20, wherein the fastening part (204) is designed for working as and be latched in
When in the reversing chamber (202), tactile and/or sound feedback that instruction fastens completely are provided.
23. holding meanss (200) according to claim 20, wherein the holding part (206) and the fastening part
(204) by heat-barrier material.
24. holding meanss (200) according to claim 20, wherein the heat-barrier material is pyroconductivity lower than 1W/mK
Heat-barrier material.
25. holding meanss (200) according to claim 20, wherein the holding part (206) and the fastening part
(204) still stable material is formed when being at least up to 130 DEG C by temperature.
26. tempering configuration (500) of the one kind for being tempered to sample separation equipment (30), the sample separation equipment (30)
For the fluid sample in separating sample separator (10), wherein the tempering configures (500) and includes:
According to claim 1 to the holding meanss (200) for being used to keep sample separation equipment (30) described in any one of 25；
Reversing chamber (202) tempering temperature different from ambient temperature can occur in the reversing chamber (202), and
The reversing chamber (202) includes assembling device (208), and the assembling device (208) is for assembling the holding meanss (200)
The fastening part (204).
27. tempering according to claim 26 configures (500), including at least one of following features:
Including according to claim 1 at least one Further retention devices (200) described in any one of 25, wherein sample
Separation equipment (30) is maintained at different by means of the holding meanss (200) and at least one described Further retention devices (200)
Position at；
Wherein, the holding part (206) and the reversing chamber (202) are formed in this way: if sample separation equipment
(30) by the holding part (206) holding and when the holding meanss (200) are fastened in the reversing chamber (202), make
Obtaining not can be carried out contact and/or not can be carried out directly between the sample separation equipment (30) and the reversing chamber (202)
Thermally conductive connection；
Wherein, the reversing chamber (202) includes the tempering wall (210) that can be tempered, in the tempering wall (210), described group
Assembling device (208) is formed for installing the fastening part (204).
28. tempering according to claim 27 configures (500), wherein the assembling device (208) is formed as recess.
29. tempering according to claim 27 configures (500), wherein it is recessed that the assembling device (208) is formed as v-shaped
Mouth, T- shape recess or semi-circular indentation.
30. tempering according to claim 27 configures (500), wherein the assembling device (208) is formed with external point
It splits.
31. a kind of sample separator (10) for by the fluid sample separation in mobile phase at fraction, wherein the sample
Separator (10) includes:
Fluid delivery system is used to convey the mobile phase and the fluid sample；
Sample separation equipment (30), the fluid sample in mobile phase for being used to convey in the fluid delivery system
It is separated into fraction；
According to claim 1 to described in any one of 25, for the sample separation equipment (30) to be maintained at the sample
Holding meanss (200) in the reversing chamber (202) of this separator (10).
32. sample separator (10) according to claim 31, further includes at least one of following characteristics:
The sample separation equipment (30) is designed as chromatographic isolation equipment；
The sample separator (10) is configured at least one physics at least one fraction of the fluid sample, changes
It learns and/or biological parameter is analyzed；
The sample separator (10) includes by the device and liquid chromatography equipment for chemistry, biology and/or Pharmaceutical Analysis
At least one of group of composition；
The fluid delivery system (20) is configured to the mobile phase described in high drive；
The sample separator (10) is configured to microfluidic device；
The sample separator (10) is configured to receive fluid means；
The sample separator (10) includes the detector (50) for being detected to the isolated fraction；
The sample separator (10) includes the injector (40) for being injected into the fluid sample in the mobile phase；
The sample separator (10) includes the sample fractionator for being fractionated to the isolated fraction.
33. sample separator (10) according to claim 32, wherein the fluid delivery system (20) is configured to
Use mobile phase described at least 100 bars of pressure-drivens.
34. sample separator (10) according to claim 32, wherein the fluid delivery system (20) is configured to
Use mobile phase described at least 500 bars of pressure-drivens.
35. sample separator (10) according to claim 32, wherein the fluid delivery system (20) is configured to
Use mobile phase described at least 1000 bars of pressure-drivens.
36. sample separator (10) according to claim 32, wherein the sample separation equipment (30) is designed as color
Compose splitter.
37. sample separator (10) according to claim 32, wherein described by for chemistry, biology and/or drug
At least one of the group of device and the liquid chromatography equipment composition of analysis is HPLC device.
38. method of the one kind for manufacturing holding meanss (200), the holding meanss (200) are used for sample separation equipment
(30) it is maintained in reversing chamber (202), the sample separation equipment (30) is for the fluid in separating sample separator (10)
Sample, wherein the described method includes:
It is formed fastening part (204), the tightening member is used to the holding meanss (200) being fastened on the reversing chamber
(202) at；
It is formed holding part (206), the holding part (206) connect in this way with the fastening part (204): institute
Holding part (206) are stated to be formed for accommodating various sizes of sample separation equipment (30)；
The holding part (206) is set to be formed as softer than the fastening part (204).
Wherein, the holding part (206) includes multiple elastic holding structures for keeping the sample separation equipment (30)
(300),
Wherein, the holding structure is selected from by the group of thin slice, brush, fritter, tooth or at least one pore structure composition.
39. the method according to claim 38 for manufacturing holding meanss (200), wherein the thin slice is different
The thin slice of longitudinal extension and/or thickness.
40. the method according to claim 38 for manufacturing holding meanss (200), wherein the pore structure is sponge.
41. the method according to claim 11, including one of following characteristics:
Wherein, the holding part (206) is formed by means of at least part of fastening part described in double teeming (204)；
Wherein, the holding part (206) and the fastening part (204) manufacture with being separated from each other, and are then connected to each other.
42. according to the method for claim 41, wherein the holding part (206) described in injection molding double teeming by means of fastening
Partially at least part of (204) is formed.
43. according to the method for claim 41, wherein the holding part (206) and the fastening part (204) are respectively
It is manufactured with being separated from each other by means of injection molding.
44. according to the method for claim 41, wherein the holding part (206) and the fastening part (204) are subsequent
Friction lock and/or shape lock it is connected to each other.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102014115807.5A DE102014115807A1 (en) | 2014-10-30 | 2014-10-30 | Holding device for sample separation devices with sections of different strength |
| DE102014115807.5 | 2014-10-30 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105572277A CN105572277A (en) | 2016-05-11 |
| CN105572277B true CN105572277B (en) | 2019-10-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510719519.XA Active CN105572277B (en) | 2014-10-30 | 2015-10-29 | The holding meanss of part for sample separation equipment, with varying strength |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN105572277B (en) |
| DE (1) | DE102014115807A1 (en) |
Families Citing this family (1)
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|---|---|---|---|---|
| DE102021106086A1 (en) | 2021-03-12 | 2022-09-15 | Agilent Technologies, Inc. - A Delaware Corporation - | Temperature control of a chromatographic column |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03170863A (en) * | 1989-11-29 | 1991-07-24 | Sekisui Chem Co Ltd | Heat block for column oven of chromatograph |
| EP0545843A1 (en) * | 1991-12-03 | 1993-06-09 | Tracer Analitica S.L. | Fixing arrangement for chromatography columns |
| JP2000039428A (en) * | 1998-07-21 | 2000-02-08 | Tosoh Corp | Column thermostatic chamber |
| CN201600354U (en) * | 2009-12-22 | 2010-10-06 | 上海悦胜芜湖药业有限公司 | Column oven |
| CN202822863U (en) * | 2012-08-27 | 2013-03-27 | 宁波检验检疫科学技术研究院 | Embedded type solid phase extraction device |
| CN103376298A (en) * | 2012-04-18 | 2013-10-30 | 株式会社岛津制作所 | Column oven for liquid chromatograph |
| WO2013167193A1 (en) * | 2012-05-10 | 2013-11-14 | Agilent Technologies, Inc. | Two-dimensional fluid separation with controlled pressure |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3785207T2 (en) | 1987-09-26 | 1993-07-15 | Hewlett Packard Gmbh | PUMP DEVICE FOR DISPENSING LIQUID AT HIGH PRESSURE. |
| EP0984276A4 (en) * | 1998-02-16 | 2003-08-27 | Inst Physical & Chem Res | Capillary cassette and method of producing the same |
-
2014
- 2014-10-30 DE DE102014115807.5A patent/DE102014115807A1/en active Pending
-
2015
- 2015-10-29 CN CN201510719519.XA patent/CN105572277B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03170863A (en) * | 1989-11-29 | 1991-07-24 | Sekisui Chem Co Ltd | Heat block for column oven of chromatograph |
| EP0545843A1 (en) * | 1991-12-03 | 1993-06-09 | Tracer Analitica S.L. | Fixing arrangement for chromatography columns |
| JP2000039428A (en) * | 1998-07-21 | 2000-02-08 | Tosoh Corp | Column thermostatic chamber |
| CN201600354U (en) * | 2009-12-22 | 2010-10-06 | 上海悦胜芜湖药业有限公司 | Column oven |
| CN103376298A (en) * | 2012-04-18 | 2013-10-30 | 株式会社岛津制作所 | Column oven for liquid chromatograph |
| WO2013167193A1 (en) * | 2012-05-10 | 2013-11-14 | Agilent Technologies, Inc. | Two-dimensional fluid separation with controlled pressure |
| CN202822863U (en) * | 2012-08-27 | 2013-03-27 | 宁波检验检疫科学技术研究院 | Embedded type solid phase extraction device |
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| Publication number | Publication date |
|---|---|
| DE102014115807A1 (en) | 2016-05-19 |
| CN105572277A (en) | 2016-05-11 |
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