CN105572277A - Maintaining apparatus for sample separating device and having parts with different strengthes - Google Patents
Maintaining apparatus for sample separating device and having parts with different strengthes Download PDFInfo
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- CN105572277A CN105572277A CN201510719519.XA CN201510719519A CN105572277A CN 105572277 A CN105572277 A CN 105572277A CN 201510719519 A CN201510719519 A CN 201510719519A CN 105572277 A CN105572277 A CN 105572277A
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- sample separation
- holding device
- retaining part
- fastening part
- reversing chamber
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6047—Construction of the column with supporting means; Holders
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The invention relates to a maintaining apparatus (200) for a sample separating device and having parts with different strengthes. The maintaining apparatus (200) serves to maintain a sample separating device (30) inside a reversing chamber. The sample separating device (30) serves to separate a fluid sample inside a sample separating apparatus (10). The maintaining apparatus (200) includes: a fastening part (204) which is designed to fasen the maintaining apparatus (200) inside the reversing chamber, a maintaining part (206) which is connected to the fastening part (204) and is designed to maintain the sample separating device (30) which has different sizes. The maintaining part (206) is desiged to be softer than the fastening part (204).
Description
Technical field
The present invention relates to a kind of holding device, back-fire arrangement, sample separation device and the method for the manufacture of holding device.
Background technology
In HPLC, liquid (mobile phase) usually with accurate controlled flow velocity (such as, in the scope of several microlitre per minute to several milliliters) and under high pressure (usually from 20 bar to 1000 Palestine and Israels, reach 2000 bar at present) move through Stationary liquid (such as, chromatographic column), to be separated the independent component of the sample liquid be incorporated in mobile phase, under high pressure, the compressibility of liquid is apparent.This HPLC system is at the EP0 of same Applicant Anjelen Sci. & Tech. Inc (AgilentTechnologies, Inc.), known in 309,596B1.
During detachment process, be necessary or desirable under post being placed in suitable temperature.In order to this object, post to be arranged in column oven and to heat in column oven.Post is arranged in column oven normally uncomfortable for the user, and often causes detachment process interference, uneven post heating.
Summary of the invention
The object of the invention is to improve flexibility, process sample separation equipment and obtain accurate separating resulting.This object is solved by independent claims.Other embodiment is illustrated by dependent claims.
According to exemplary embodiment of the present invention, provide a kind of holding device, this holding device is used for sample separation equipment to remain in reversing chamber, sample separation equipment is for separating of the fluid sample in sample separation device, wherein, holding device comprises fastening part, and it is designed to holding device to be fastened in reversing chamber; Retaining part, it is connected with fastening part, and retaining part is designed to the sample separation equipment keeping having different size, and wherein, retaining part is designed to softer than fastening part.
According to another exemplary embodiment, provide a kind of tempering configuration, this tempering is configured for carries out tempering to the sample separation equipment for separating of the fluid sample in sample separation device, wherein, tempering configuration comprises holding device and reversing chamber, holding device has above-mentioned feature and for keeping sample separation equipment, can form the temperature different from ambient temperature in reversing chamber, and reversing chamber comprises the erecting device of the fastening part for installing holding device.
According to another exemplary embodiment, provide a kind of sample separation device, this sample separation device is used for the fluid sample separation in mobile phase to become fraction, and wherein, sample separation device comprises fluid delivery system, and it is for transport flow phase and fluid sample; Sample separation equipment, it is for becoming fraction by the fluid sample separation in the mobile phase carried by fluid delivery system; And holding device, it has above-mentioned feature, for remaining in the sample separation equipment in the reversing chamber of sample separation device.
According to another exemplary embodiment, provide a kind of method for the manufacture of holding device, this holding device is used for sample separation equipment to remain in reversing chamber, sample separation equipment is for separating of the fluid sample in sample separation device, wherein, pass through the method, being designed for by fastening part is fastened on reversing chamber by holding device, the retaining part be connected with fastening part designs by this way: retaining part is designed to the sample separation equipment keeping different size, and retaining part is softer than fastening part.
In the scope of the application, understand term " soft " especially by this way: the physical strength of the fastening part that softer retaining part is harder is less, therefore when the power of applying, retaining part is larger than fastening part contractibility.Specifically, retaining part can be surrendered under application of a force and can change its profile during being continuously applied power, the value of applied force is in the scope of the muscle strength of mankind user, but, during the impact of this power, the size of fastening part is roughly stable, that is, keep its profile.Therefore, retaining part can be designed as soft-component and fastening part can be designed as hard component.Term " soft " refers specifically to retaining part stability low compared with fastening part.Stability is mechanical material characteristic, it is characterized in that the ability that material repels is out of shape.Stability refers to the tension force calculated by maximum, force, and this maximum, force obtains in defined strain place relevant to the former cross-sectional area of material.
The advantage that exemplary embodiment of the present invention has is: fastening part can by rigidly and size is fixedly secured in reversing chamber, and therefore not easily lose, but when retaining part attaching sample separation equipment, the shape of the retaining part adjoined with fastening part is adapted to the shape of sample separation equipment, and but confining force is applied on sample separation equipment, thus attaching sample separation equipment in a reliable fashion.The biddability of retaining part and compliance allow retaining part to be automatically adapted to the sample separation equipment of different size, and therefore retaining part can be widely used in the sample separation experiment differed greatly, and the experiment of this sample separation has different requirements for the size of used sample separation equipment.By keeping the sample separation device inserted to be spaced, not contact with the tempering wall of reversing chamber by means of retaining part and fastening part, can guarantee fully or at least mainly by means of the heat interchange between sample separation equipment and ambient atmosphere but not by means of the direct heat transfer of the heated wall of reversing chamber, tempering being performed to sample separation equipment.Therefore, in the inside of sample separation equipment, guarantee to carry out tempering equably to sample separation equipment, and there is no larger thermograde, avoid the measurement illusion caused by heat whereby.Due to the environment roughly insulated that (particularly by means of liquid phase chromatography) produces, accurate separating resulting can be obtained.Fastening part is stablized retaining part, is made it easy to operation and provide fastening function in reversing chamber.Retaining part is allowed for the sample separation equipment being applicable to different size pliable and toughly, and particularly provides sufficiently high friction force and/or holding force by means of friction lock and/or holding function, for firmly keeping sample separation equipment.
Below, the more embodiments of holding device, tempering configuration, sample separation apparatus and method will be described.
According to embodiment, retaining part can be formed by the material softer than fastening part.As a result, according to this embodiment, retaining part and fastening part can be made up of different materials, and make with regard to they respective functions, two parts can adapt in the mode that material technology is special.Specifically, the material with different physical strength can be used for retaining part on the one hand, and can be used for fastening part on the other hand.
According to embodiment, retaining part is at least formed by the material identical with fastening part at some parts, and at least low than the strength of materials of fastening part in some positions.Therefore, specifically, the embodiment of a part for holding device is also fine, and wherein, can be obtained fastening part and the retaining part of different physical strength by the different strengths of materials (specifically, material thickness and/or material shape).The characteristic that the lower strength of materials causes the higher strength of materials softer, and vice versa.Along whole fastening part and along whole retaining part or only in some part or the strength of materials of specifying these different in some position.The lower strength of materials of some position can be enough to produce elasticity or mechanically deform (such as, thinner part, specifically, its in maintaining part office as reversible predetermined kinking point).
According to embodiment, retaining part can be formed by resilient material and fastening part can be formed by rigid material.Resilient material can be understood as such material (specifically, contrary with plastic deformable material): produce by making specific material area be inclined to the non-forced position of its remainder the reset force be in proportion with deflection, thus demonstrate the characteristic of desirable spring.On the contrary, rigid material can be understood as such material: the muscle strength produced when user is applied through operation sample separation equipment can not practical function be significantly out of shape.
According to alternate embodiment, that retaining part and fastening part can be formed as being separated and parts be connected to each other, the parts be connected to each other with particularly forming locking ground and/or friction lock.According to this embodiment, first, retaining part and fastening part can to make the manufacture independent of each other of the optimized mode of their specific function and to be connected to each other subsequently.Specifically, this embodiment is allowed for as retaining part and fastening part select different materials.Can be connected with fastening part retaining part by means of only such as clogging mutually, thus form support connection.
According to embodiment, fastening part and/or retaining part can comprise plastics.Or fastening part and/or retaining part can be manufactured by other material, such as, pottery or metal.
According to embodiment, fastening part can be formed by the material in the group be made up of polyamide, glass filled polyamide, acronitrile-butadiene-styrene, tygon and teflon.These materials have the intensity being enough to guarantee reliably to be fastened on by holding device in reversing chamber.In addition, these materials have enough low pyroconductivity, to be prevented from by fastening part conducting heat in large quantities between reversing chamber and sample separation equipment.Similarly, these materials are applicable to make to manufacture fastening part in cost-effective manner.
According to embodiment, retaining part can be formed by the material in the group be made up of thermoplastic elastomer, rubber, silicone, ethylene-propylene-diene rubber and dimethione.These materials comprise enough mechanically deforms for the sample separation equipment making the profile of retaining part be adapted to the size just used in a reliable fashion.Meanwhile, these materials have enough low pyroconductivity, to be prevented from by retaining part conducting heat in large quantities between reversing chamber and sample separation equipment.In addition, these materials are applicable to make to manufacture retaining part in cost-effective manner.
According to embodiment, fastening part can be designed for without the need to instrument ground, is particularly manually fastened in reversing chamber by holding device.Fastening part is dismantled, so higher users'comfort can be obtained because fastening part to be installed in reversing chamber or from reversing chamber without the need to instrument by user.Such as, fastening part to be only arranged in reversing chamber and rotational fastener part just can be enough to holding device to be firmly fastened in reversing chamber subsequently by user.
According to embodiments of the invention, fastening part can be designed for and reversibly be fastened in reversing chamber.In other words, fastening part can (such as, in different positions) engage several times and again remove from this in reversing chamber.In addition, holding device can be used in some different reversing chambers.The reversible availability of holding device makes it be suitable for flexibly and general.
According to embodiment, when fastening part is fastened in reversing chamber completely, when being particularly latched in reversing chamber, fastening part is designed for the sense of touch and/or sound feedback that provide instruction completely fastening.In other words, when holding device is successfully fastened in reversing chamber completely, provide holding device to be now attached to obvious tactile feedback in reversing chamber safely to user.The comfort level and simultaneously providing that this improves user is effectively protected in order to avoid apply too large fastening force by user, when there is this power, may damage holding device and/or reversing chamber.Audibly (such as, use can hear ticktack) fastener cycle can be completed to user's prompting.
According to embodiment, fastening part can comprise rotating closed device, and this rotating closed device is used for the relatively rotating closed device place rotational fastener at reversing chamber.Be characterised in that and holding device be attached on reversing chamber and the fastener cycle subsequently holding device being screwed to restraint location place is simple for the user and can knows intuitively.Meanwhile, this rotational fastener prevents holding device from throwing off undesirably when each pulling and thus improving fastening stability.Alternatively, or additionally, fastening part can be clamped in the erecting device of reversing chamber.
According to embodiment, rotating closed device comprises teat, this teat has the radial direction changed in the circumferential and extends, and rotating closed device can be fastened in the recess of relatively rotating closed device of reversing chamber in the mode of shape locking (form-locking) and/or friction lock by rotary actuation.If at the cyclic lug that the fastening area formation external diameter of fastening part circumferentially changes, so by the circumferential section with small radii being placed in indent and subsequently another circumferential section with larger radius being screwed to recess, holding device just can be realized to be fastened in reversing chamber.By using simple mechanical hook-up, simple fastener cycle combines with the fastening of high reliability.
According to embodiment, retaining part can be designed for and keep the sample separation equipment of diameter at least between 3mm to 12mm.Specifically, for chromatographic resolution experiment, separating column has described size usually, and uses same generally available holding device to keep these posts according to described embodiment subsequently.Due to holding device enough soft, surrender and flexible design, the material of holding device tightens up the sample separation equipment of different size, and by keeping this to tighten in conjunction with elastic reset power and friction force between retaining part and sample separation equipment, specifically, rubber retaining part is used especially effectively can to apply power.
According to embodiment, fastening part can comprise base portion and bearing part, and wherein, base designs is used for being fastened in reversing chamber and retaining part is arranged in bearing part.Base portion and bearing part can be located relative to one another, and guarantee to leave spacing between sample separation equipment and reversing chamber.This impels temperature to be uniformly distributed along the whole extension of sample separation equipment.
According to embodiment, retaining part angularly, particularly with the angle between 30 ° to 150 °, is more particularly arranged in bearing part with the angle between 60 ° to 120 °.Such as, retaining part vertically extends from the load-bearing surface of the carrying bearing part of retaining part.Specifically, the outer end avoiding reversing chamber of retaining part and bearing part angled.Thus such as before retaining part, forming cavity, this cavity is beak and this cavity forms tapering towards bearing part, and this cavity impels intuitively and is inserted in retaining part by the sample separation equipment with different-diameter easily.
According to embodiment, retaining part comprises the multiple elastic holding structures for keeping sample separation equipment.Such as, these holding structures can comprise thin slice (lamella) (particularly having the thin slice of different extending longitudinally and/or thickness), brush (particularly having the brush of different extending longitudinally and/or thickness), burling (burling) (particularly having the burling of different extending longitudinally and/or thickness), tooth (particularly having the tooth of different extending longitudinally and/or thickness) and/or at least one pore structure (particularly at least one sponge).By providing, wherein to have in these holding devices of cavity multiple, can improve the adaptability of holding device to the sample separation equipment of different size further.
According to embodiment, holding structure angularly, particularly with the angle between 30 ° to 150 °, more particularly extends from bearing part with the angle between 60 ° to 120 °.Such as, holding structure vertically can stretch from bearing part.When holding structure is in tilted layout from bearing part, can be the visible groove that intuitively insert of user's formation for inserting respective sample separation equipment.
According to embodiment, holding device can be formed as the sample separation equipment for keeping different size based on holding force and/or friction force.Holding force and friction force be allowed for reversibly installing and dismantle different size sample separation device and simultaneously for reliably keeping.
According to embodiment, retaining part and/or fastening part are by the material of heat transfer rate variance or heat insulator, and the material particularly with the pyroconductivity being less than 1W/mK is formed.Specifically, holding device and reversing chamber can be formed by this way: if holding device keeps sample separation equipment and holding device is fastened in reversing chamber, make can not to contact between sample separation equipment and reversing chamber and/or directly heat transfer connects.According to these preferred embodiments, remain on sample separation equipment in holding device can by means of with regard to thermal conductivity at least some part heat insulation or heat transfer rate variance holding device roughly with the heated wall pyrolysis coupling of reversing chamber.Utilize reversing chamber (it such as can comprise heated wall) to carry out tempering to sample separation equipment to realize mainly through the air in reversing chamber, tempering (particularly heating) can be carried out by reversing chamber to its part.Therefore, guarantee that sample separation device equably and spatially tempering equably, which increases the precision of separating resulting.
According to embodiment, retaining part and fastening part, are particularly formed at least to material still stable when 130 ° at least to 110 ° by temperature.Therefore, holding device can resist the prevailing temperature in the column oven of modern liquid chromatography device.
According to embodiment, tempering configuration can at least comprise the Further retention devices with the above-mentioned feature mentioned, wherein, by means of holding device and at least one Further retention devices (especially by means of two identical holding devices of structure), sample separation equipment is remained on different positions.Specifically, can keep roughly in a tubular form in the region of its two ends respect to one another and cylindrical sample separation equipment by means of respective holding device, make the applying power on holding device and sample separation equipment enough low and spatially distribute equably.
According to embodiment, reversing chamber can comprise temperable tempering wall, at tempering wall place, apparatus for assembling is formed for installing fastening part, be formed as recess especially, be more particularly formed as V-arrangement recess, T-shaped recess or semi-circular indentation, there is outside wedge especially especially.Be particularly advantageously, at least one guide rail describes that receiving recess can be arranged on tempering wall, this recess is formed for and is applicable at large and at an arbitrary position place installing one or more holding device (and/or other parts, such as, for preheating the preheating apparatus of the fluid of the upstream of sample separation equipment).Such as, can be configured to hold the different region of two of sample separation equipment (namely along holding two spaced holding devices of the direction extending longitudinally of recess, end regions), the different sample separation equipment of accommodation two (it can also have different sizes) can be configured to and/or can be configured to hold sample separation equipment and other fluidic component (such as, for heating the preheating apparatus of the upstream of sample separation equipment).Therefore, can provide defined by the user, hold the modular system of some similar and/or different fluidic components, in reversing chamber, particularly provide these to hold in recesses several.
It is particularly advantageous for having proved that V-arrangement accommodation recess combines with outside wedge.This such as shown in fig. 14 structure allows first fluid parts (such as, preheating apparatus) be contained in V-arrangement accommodation recess, directly (particularly can heat transfer) thermal coupling at the area of heating surface place of tempering wall, and allow such as by second fluid parts (simultaneously, sample separation equipment) be contained in outside wedge place, favourable indirect (gas transport by means of in reversing chamber) thermal coupling is only had at the area of heating surface place of tempering wall.Because between these fluidic components, fluid access path is shorter, this makes the layout of the fluidic component in reversing chamber save space.Arrange V-arrangement to hold recess and additionally provide such advantage: when user sample separation equipment is directly inserted into undesirably V-arrangement hold in recess time (this can make undesirably sample separation equipment in position contact with reversing chamber directly and therefore heat unevenly), sample separation equipment can skid off V-arrangement accommodation recess under gravity and thus prevent user from operating sample separation equipment in inappropriate mode.Therefore, parasitic direct heating is impossible.
Or holding device can also (reversibly or irreversibly) be bonded in reversing chamber.
According to embodiment, retaining part can by means of double teeming, the particularly formation at least partially of injection moulding double teeming fastening part.Such as, first, fastening part is manufactured according to the first injection molding process.Subsequently, the fastening part manufactured in like fashion can be inserted in another injection mo(u)lding room and to use shaping for the formation of the material injection of retaining part.This guarantees the compact siro spinning technology between retaining part and fastening part, and but allows the material alone of retaining part and fastening part to be applicable to the different function of retaining part and fastening part.
According to another embodiment, retaining part and fastening part can manufacture separated from one anotherly, particularly respectively by means of injection mo(u)lding separated from one another manufacture, and can be connected to each other subsequently, to be particularly connected to each other to friction lock and/or shape locking.Such as, can at the indoor manufacture retaining part of the first injection mo(u)lding.In addition, can at the indoor manufacture fastening part of the second injection mo(u)lding.The part manufactured in like fashion can be assembled into holding device subsequently.This such as by forming mechanical detent or other connection, or also mechanically can be manufactured by pure by use bonding or by means of joint method (such as, welding).
According to embodiment, sample separation device can be configured to micro-fluid measurement device, liquid-chromatography apparatus or HPLC.Sample separation device especially can be configured to HPLC device (high performance liquid chromatography), life science apparatus or SFC device (supercritical fluid chromatography).Certainly, other application is also fine.
According to embodiment, sample separation device can be configured to withstand voltagely to operate under the pressure reaching about 100 bar, especially for operating under the pressure reaching about 500 bar, operates more specifically under the pressure reaching about 1000 bar.
According to embodiment, the sample separation device as sample separation equipment can comprise separating column, and this separating column is for separating of the different fractions of injected fluid sample.This separating column can be filled with absorbing medium, such as, and the porous bead be made up of silica gel or activated charcoal.By the chemical reaction with this porous bead, fluid sample can by temporary fixing or adsorbed in separating column.Such as, by the gradient of setting solvent composition, independently fraction can be dissolved independently and remove absorption from adsorbing medium, and can be detected subsequently.
According to embodiment, analyze fluid sample and mobile phase that pump is designed for conveying injection.Mobile phase can be solvent composition, and this solvent composition can be temporarily constant or change in adjustable mode, and is being injected into after in sample separation path by fluid sample by means of sampling valve, and this solvent composition will mix with fluid sample.The potpourri of mobile phase and fluid sample will be pumped across chromatographic resolution path by means of the analysis pump being formed as high-pressure pump.Sample separation device can also comprise one or more pump, and this one or more pump is used for the fluid sample of injection to be conducted through sample separation device at least partially together with mobile phase.This pump can be configured to such as under high pressure (such as, in a few hectobar to 1000 Palestine and Israel), mobile phase is pumped across system.
According to embodiment, sample separation device can comprise the sample detector of the sample component for detecting be separated fluid sample.This sample detector can based on the Cleaning Principle detecting the electromagnetic radiation (such as, in the field of UV scope or visible range) that be produced by the predetermined fluid composition of fluid sample.
Alternatively or additionally, measurement mechanism can comprise the sample fractionator (fractionator) for carrying out fractionation to be separated sample component.Different sample component can such as be transported in different containers by this fractionator.The fluid sample analyzed also can be supplied to waste canister.
Accompanying drawing explanation
By reference to below in conjunction with the detailed description of accompanying drawing to embodiment, more easily can be familiar with and understand other object of embodiments of the invention and many added benefit better.In essence or functionally same or similar feature represents with same Reference numeral.
Fig. 1 illustrates the HPLC-measurement mechanism according to exemplary embodiment of the present invention.
Fig. 2 and Fig. 3 illustrates two space view of the holding device according to exemplary embodiment of the present invention.
Fig. 4 illustrates the fastening part of the holding device according to exemplary embodiment of the present invention.
Fig. 5 and Fig. 6 illustrates and to configure according to the tempering of exemplary embodiment of the present invention.
Fig. 7 to Figure 12 illustrates the holding device according to exemplary embodiment of the present invention.
Figure 13 illustrate according to exemplary embodiment of the present invention, the side view of the tempering configuration of it being installed sample separation equipment.
Figure 14 illustrates the tempering wall and erecting device that configure according to the tempering of exemplary embodiment of the present invention.
Figure 15 illustrates the detail view of a part for the reversing chamber according to Figure 14.
Figure 16 and Figure 17 illustrates and to configure according to the tempering of exemplary embodiment of the present invention.
Diagram in accompanying drawing is exemplary.
Embodiment
Before describing exemplary embodiment of the present invention with reference to the accompanying drawings, propose some basic problems, launch exemplary embodiment of the present invention based on these basic problems.
According to exemplary embodiment, propose a kind of post retainer, it is arranged on the inside of chromatographic column incubator, for the post keeping different-diameter in a reliable fashion as clamping and grip device.After installation, the position that the user that holding device rests on column oven limits, and chromatographic column mounted thereto is held in place.After user installs, can prevent holding device and post from less desirable movement occurring.Inside holding device being arranged on column oven is very simple and without the need to instrument.Holding device such as can be arranged in wedge (dovetail) recess of column oven, moves along these recesses, and is therefore applicable to the post of different length together with other holding device.Due to the flexible construction of holding device, therefore, it is possible to adapt to different column diameters.Owing to being monolithic design according to the holding device of embodiment, user can prevent complicated multi-part device from losing small part in a reliable fashion.Replace wedge recess, can utilize undercutting in reversing chamber, to hold holding device, this undercut cross-sectional is T-shaped or triangle or (partly) circle.In addition, the wall (that is, planar wall) of reversing chamber can place erecting device highlightedly.Holding device can be provided with twist lock mechanism to allow rotational fastener.If this holding device to be arranged in recess and rotated, holding device will be fixed in recess.This allows holding device freely to locate along recess.But if holding device is arranged in recess, holding device will be fixed in recess in the mode loaded with regard to displacement and slip, until user discharges spin locking and other are fixing also in new ways along accommodation recess positioning holding device.This can implement several times and without the need to using instrument.After holding device being installed to accommodation recess, simply post can being inserted into the receiving breach at the outside available position place being arranged in holding device or the retaining part of other structure, wherein also can not using this process of execution of instrument.By post being taken out from retaining part the dismounting implementing post.Due to the flexibility of the front side of holding device, can in a reliable fashion all posts with different-diameter be held in place, make without the need to different model for stratographic analysis object holding device.The holding device of design can hold different posts.By the flexibility and elasticity that use the spring material of flexible metal, expect by using rubber type of material etc. that retaining part can be made to obtain.Suitable shaping (such as, holding structure being designed to thin slice, tup, brush, blade, ball etc.) can contribute to the flexibility of the material (suitable such as polymkeric substance) for retaining part.This holding device can reversibly bend in like fashion and/or fasten and go for different column diameters, wherein can prevent post from moving undesirably under attachment state simultaneously.Holding device is preferably formed by heat insulator, so that the adiabatic temperature of not offsetting coupled columns controls.
Fig. 1 illustrates the theory structure of the HPLC-system for sample separation device 10 exemplarily, and this sample separation device such as can be used for liquid phase chromatography.Analyze the tripping device that pump 20 drives mobile phase to pass through as sample separation equipment 30, provide mobile phase by dissolution vessel 25 and discharged the gas in mobile phase by deaerator 27, in sample separation equipment, comprising Stationary liquid.Sample input block (being also referred to as injector or injector) 40 is arranged between pump 20 and sample separation equipment 30, is injected in mobile phase by fluid sample for the fluid switch (it can count (countedto) injector 40) by means of exemplary embodiment according to the present invention.The Stationary liquid of sample separation equipment 30 is intended to the sample component of separating sample liquid.Detecting device 50 detects the sample component of the sample be separated, and fractionation plant 60 may be used for the sample component of be separated sample liquid such as being put into the container for it provides or outlet.Control device 70 controls the parts of sample separation device 10.
Under although the liquid path between pump 20 and sample separation equipment 30 is in high pressure usually, but sample liquid is imported into the region be separated with liquid path at first at ambient pressure, this region is the sample loop (as sample receiving volume) of so-called injector 40, itself then sample liquid is input in the liquid path be under high pressure.When the sample liquid be at first under normal pressure in sample loop is linked into the liquid path under high pressure, the inclusions unexpected (usually in the scope of millisecond) of sample loop reaches the pressure in the system of sample separation device 10.
In FIG, it can also be seen that sample separation equipment 30 to be arranged in reversing chamber 202 and can be heated in reversing chamber by heating source 80 together with preheating apparatus 90.More particularly, heating source 80 heats the wall of reversing chamber 202.On the wall that preheating apparatus 90 is directly arranged in reversing chamber 202 and thus directly and wall thermal coupling, fluid flowed through preheating apparatus 90 before flowing into sample separation equipment 30.Contrary with preheating apparatus, be arranged on the wall of reversing chamber 202 to sample separation equipment 30 heat insulation, and therefore the elevated temperature gas heated by the heated wall in reversing chamber 202 indirectly and heat heat insulationly.
Fig. 2 and Fig. 3 illustrates the different views of the holding device 200 according to exemplary embodiment of the present invention, can be installed in reversing chamber 202 by sample separation equipment 30 by this holding device 200.
Holding device 200 is for remaining on sample separation equipment 30 in the reversing chamber 202 according to the sample separation device 10 of Fig. 1.Reversing chamber 202 comprises air chamber 270 and temperable tempering wall 210, and on tempering wall, erecting device 208 is formed with the form with the V-arrangement recess of outside wedge, and outside wedge is for installing the fastening part 204 of holding device 200.
Holding device 200 is also formed by fastening part 204 and retaining part 206, fastening part forms the holding device 200 be used in fastening reversing chamber 202, and retaining part is connected with fastening part 204, this retaining part forms the sample separation equipment 30 for keeping different size (particularly having different-diameter), wherein only keeps a specific sample separation equipment 30 at each time point.Retaining part 206 softer than fastening part 204 (that is, there is lower stability).Therefore, elastic holding part 206 obtains the shape being applicable to the sample separation equipment 30 holding different size, and can not lose the ability being fixed this sample separation equipment 30 in a reliable fashion by holding force and/or friction force.Contrary with elastic holding part, comparatively the fastening part 204 of rigidity (that is, having higher stability) is formed by this way: even if it still keeps dimensionally stable and therefore prevents holding device 200 from dropping out from reversing chamber 202 and/or the erecting device in reversing chamber 202 208 undesirably under the impact of fastening force.
Retaining part 206 is such as formed by silicones and is therefore formed by the material that the fastening part 204 of ratio as being made up of polyamide is softer, and retaining part is also called as soft-component, and this fastening part can be called as hard component.The Material selec-tion mentioned also causes good heat insulation between sample separation equipment 30 and reversing chamber 202, and sample separation equipment remains in retaining part 206, and holding device 200 is arranged in reversing chamber place by means of fastening part 204.Therefore, it is possible to guarantee the ambient temperature that sample separation equipment 30 is advantageously only heated properly, make sample separation equipment 30 in thermal insulation and work under the condition that therefore boundary is clear and definite.In addition, these materials are applicable to resist the heating-up temperature (such as, reaching 110 DEG C) existed in the reversing chamber 202 of testing for chromatographic resolution.In addition, as shown in Figure 3, compared with fastening part 204, retaining part 206 is formed in the lower region of the strength of materials.In addition, retaining part 206 and fastening part 204 are discretely and the parts formed a fixed connection mutually.
As shown in Figures 2 and 3, retaining part 206 comprises continuous breach 234, under the state that holding device 200 is installed to reversing chamber 202, this continuous breach extends along the corresponding accommodation recess 236 of erecting device 208, and sample separation equipment 30 also extends along the corresponding recess 236 that holds in the mounted state.When being inserted in retaining part 206 by sample separation equipment 30, breach 234 expands, thus allows to hold sample separation equipment 30.In addition, the resilient material of retaining part 206 applies clamping fastener and the power of friction fastening sample separation equipment 30 at the both sides place of breach 234.Retaining part 206 comprises the multiple elastic holding structures 300 for keeping sample separation equipment 30, and in an illustrated embodiment, elastic holding structure is formed as the thin slice be arranged symmetrically with free end at the both sides place of breach 234.These holding structures 300 can significantly or slightly launch according to the size of the sample separation equipment 30 held separately or flexibly bend or rather, and this holding structure is mutual one-sided connection in groups at wider coupling part 302 place.Have the design of described retaining part 206, retaining part 206 is applicable to keep the sample separation equipment 30 in the scope of diameter between 3mm to 12mm.According to the size of sample separation equipment 30 kept, significantly or slightly enlargement indentation 234.Can artificially and independent sample separation equipment 30 being inserted in retaining part 206 without instrument, and artificially and independent sample separation device being taken out from retaining part 206 without instrument again subsequently.
Especially before sample separation equipment 30 is inserted into retaining part 206, make fastening part 204 in manually and reversibly mode (namely, can again to discharge and again fastening) be formed in erecting device 208 place of reversing chamber 202, fastening part is used for tightness retaining mechanism 200 and without the need to instrument.This can realize by means of rotational shell mechanism, is explained in more detail it with reference to Fig. 4.Fastening part 204 has reversing chamber side base portion 220 and sample separation equipment side bearing part 222.The erecting device 208 that base portion 220 is formed in reversing chamber 202 is sentenced for fastening.Retaining part 206 is arranged in bearing part 222 place and patchery (patch) wherein.Preferred fastening part 204 is formed as when being fastened on completely in reversing chamber 202 (, when being locked in reversing chamber 202 place) sense of touch that provides instruction completely fastening (, shock can be felt at end position place) sound (that is, can shock be heard) feedback.Retaining part 206 vertically extends from bearing part 222.In addition, holding structure 300 vertically extends from bearing part 222.
Fig. 4 illustrates retaining part according to the holding device 200 of exemplary embodiment of the present invention and specifically illustrates the embodiment of base portion 220.According to Fig. 4, fastening part 204 comprises rotating closed device 400, and this rotation can form rotational fastener in the device relatively rotating closed device place that is used for the erecting device 208 of the formation wedge recess of visible, reversing chamber 202 in fig. 2.Rotating closed device 400 comprises teat, teat has different in a circumferential direction radical length r, R (wherein, r < R), revolving actuator can be used with shape locking and/or the mode of friction lock, this teat to be fastened on the indent of the relatively rotating closed device of reversing chamber 202.The initial mode being furnished with enough tolerances with the region with small radii r, is inserted into holding device 200 in the relatively rotating closed device of the erecting device 208 forming wedge recess, and thus is suitable for removably being arranged in wedge recess.After wedge recess is holding device 200 selection expectation installation site, the sense of rotation that can mark drawings attached mark 402 in Fig. 4 rotates holding device 200, enter into wedge recess until have compared with the region of major radius R, and therefore cause in reversing chamber 202 based on holding force and based on friction force tightness retaining mechanism 200.
Fig. 5 and Fig. 6 illustrates the tempering configuration 500 according to exemplary embodiment of the present invention.
In sample separation device, shown tempering configuration 500 is for carrying out tempering to sample separation equipment 30, and sample separation device is for separating of the fluid sample in sample separation device 10.Tempering configuration 500 comprises some holding devices 200 respectively, and some holding devices are for keeping being arranged on the sample separation equipment 30 of some, the different size in reversing chamber 202.In reversing chamber 202, the temperature of projecting temperature can be raised by heating arrangement (not illustrating in fig. 5 and fig .).Respective reversing chamber 202 comprises respective erecting device 208, for the fastening part 204 installing holding device 200.
The sample separation equipment 30 of the different size shown in Fig. 5 by means of two independently special holding device 200 remain on independently two different positions respectively.
Fig. 6 illustrates the different holding devices 200 with different holding structures 300, that is, one has thin slice, and one has teeth portion, and one has toward each other and two jaws mated, and one has flat spring structure.
Fig. 7 to Figure 12 illustrates the holding device 200 according to exemplary embodiment of the present invention.Holding device 200 shown in Figure 7, the fastening part 204 of holding device is rigidity and rectangular shaped and the retaining part 206 of holding device comprises two jaws respect to one another in fact in a top view.According to the holding device 200 of Fig. 8, there is the retaining part 206 similar with the holding device 200 according to Fig. 7, but according to fastening part 206 two fastening support respect to one another that the holding device of Fig. 8 comprises, there is the free end 800 widened.Holding device 200 shown in Fig. 9 is different from the holding device shown in Fig. 3 by this way, the slice-shaped holding device according to Fig. 3 with free end not only connect in groups at outside connecting portion 302 place according to Fig. 9 but also in sample separation equipment-housing region annular closed.Corresponding with the holding device of Fig. 3 in fact according to the holding device 200 of Figure 10.Holding device 200 according to Figure 11 has additional recesses 1100 on the side being supplied to sample separation equipment 30 of holding device 200.According to the holding structure 300 thin slice convex that the holding device 200 of Fig. 9 to Figure 11 comprises, and outwards recessed according to the thin slice holding structure 300 on the holding device 200 of Figure 12.
Figure 13 illustrate according to exemplary embodiment of the present invention, the side view of the tempering that it is provided with sample separation equipment 30 configuration 500.Hold recess 236 comprise roughly V-shaped, for hold preheating apparatus 90 container cavity 1300 and there is side wedge recess, the outside container cavity 1302 that can hold holding device 200.Figure 13 also illustrates that holding recess 236 is formed by this way: preheating apparatus 90 to be contained in V-arrangement container cavity 1300 and while holding device 200 be contained in outside container cavity 1302.
Figure 14 illustrates the reversing chamber 202 of the tempering configuration 500 according to exemplary embodiment of the present invention.In reversing chamber 202, the accommodation recess 236 parallel to each other of some rail shapes is provided, makes it possible to multiple fluidic component is installed.Figure 15 illustrates the detail view of a part for the reversing chamber 202 according to Figure 14.
Figure 16 and Figure 17 illustrates the tempering configuration 500 according to exemplary embodiment of the present invention.
It should be noted that term " comprises " and do not get rid of other element and multiple situations do not got rid of in article " ".In addition, the element described in conjunction with different embodiments can be combined.Should also be noted that the Reference numeral in claim should not be construed as the scope of restriction claim.
Claims (20)
1. a holding device (200), described holding device (200) is for remaining in reversing chamber (202) by sample separation equipment (30), described sample separation equipment (30) is for separating of the fluid sample in sample separation device (10), wherein, described holding device (200) comprising:
Fastening part (204), it is designed to described holding device (200) to be fastened in described reversing chamber (202);
Retaining part (206), it is connected with described fastening part (204), and described retaining part (206) is designed to the described sample separation equipment (30) keeping having different size;
Wherein, described retaining part (206) is designed to softer than described fastening part (204).
2. holding device according to claim 1 (200), comprises the one in following characteristics:
Wherein, described retaining part (206) is formed by the material softer than described fastening part (204);
Wherein, described retaining part (206) is formed by the material identical with described fastening part (204), and described retaining part (206) is at least low than the strength of materials of described fastening part (204) in some positions.
3. holding device according to claim 1 and 2 (200), wherein, described retaining part (206) is formed by resilient material, and described fastening part (204) is formed by rigid material.
4. holding device as claimed in any of claims 1 to 3 (200), comprises the one in following characteristics:
Wherein, described retaining part (206) and described fastening part (204) are formed as parts, are particularly bonded to one another formation;
Wherein, that described retaining part (206) and described fastening part (204) are formed as being separated and parts be connected to each other, the parts be connected to each other with being particularly formed as shape locking and/or friction lock.
5. holding device as claimed in any of claims 1 to 4 (200), wherein, described fastening part (204) is formed by the material in the group be made up of polyamide, glass filled polyamide, acronitrile-butadiene-styrene, tygon and teflon.
6. holding device as claimed in any of claims 1 to 5 (200), wherein, described retaining part (206) is formed by the material in the group be made up of thermoplastic elastomer, rubber, silicone, ethylene-propylene-diene rubber and dimethione.
7. holding device as claimed in any of claims 1 to 6 (200), wherein, described fastening part (204) comprises rotating closed device (400), for relatively rotating closed device (208) place of rotational fastener at described reversing chamber (202).
8. holding device according to claim 7 (200), wherein, described rotating closed device (400) comprises the teat in the circumferential with different radial directions extension (r, R), and described teat is fastened in the recess of described relatively rotating closed device (208) of described reversing chamber (202) in the mode of shape locking and/or friction lock under the effect of rotary actuation.
9. holding device as claimed in any of claims 1 to 8 (200), wherein, described fastening part (204) comprises base portion (220) and bearing part (222), wherein, described base portion (220) is designed for and is fastened in described reversing chamber (202), and described retaining part (206) is arranged in described bearing part (222) place substantially.
10. holding device according to claim 9 (200), wherein, described retaining part (206) angularly, particularly with the angle between 30 ° to 150 °, be more particularly arranged in described bearing part (222) place with the angle between 60 ° to 120 °.
11. holding devices as claimed in any of claims 1 to 10 (200), wherein, described retaining part (206) comprises the multiple elastic holding structures (300) for keeping described sample separation equipment (30).
12. holding devices according to claim 11 (200), wherein, described holding structure is selected from the thin slice by thin slice, particularly different extending longitudinally and/or thickness, brush, burling, teeth portion, or the group of at least one pore structure, particularly at least one sponge composition.
13. holding devices according to claim 12 (200), wherein, described holding structure from described bearing part (222) angularly, particularly with the angle between 30 ° to 150 °, more particularly extends with the angle between 60 ° to 120 °.
14., according to the holding device (200) in claim 1 to 13 described in any one, comprise at least one in following features:
Wherein, described fastening part (204) is designed for without the need to instrument ground, is particularly manually fastened in described reversing chamber (202) by described holding device (200);
Wherein, described fastening part (204) is designed for and is reversibly fastened in described reversing chamber (202);
Wherein, described fastening part (204) is designed for when being fastened on completely in described reversing chamber (202), particularly when being latched in described reversing chamber (202), provides the completely fastening sense of touch of instruction and/or sound feedback;
Wherein, described retaining part (206) is designed for the sample separation equipment (30) kept in the scope of diameter at least between 3mm to 12mm;
Wherein, described retaining part (206) is designed for the sample separation equipment (30) to keep different size based on the mode of holding force and/or friction force;
Wherein, described retaining part (206), particularly described retaining part (206) and described fastening part (204), by heat-barrier material, are particularly formed by the heat-barrier material of pyroconductivity lower than 1W/mK;
Wherein, when described retaining part (206) and described fastening part (204) at least reach 110 DEG C by temperature, material still stable when particularly at least reaching 130 DEG C is formed.
15. 1 kinds for carrying out tempering configuration (500) of tempering to sample separation equipment (30), described sample separation equipment (30) is for separating of the fluid sample in sample separation device (10), wherein, described tempering configuration (500) comprising:
According to the holding device (200) for keeping sample separation equipment (30) in claim 1 to 14 described in any one;
Reversing chamber (202), in described reversing chamber (202), the temperature being different from ambient temperature can be there is, and described reversing chamber (202) comprises apparatus for assembling (208), described apparatus for assembling (208) is for assembling the described fastening part (204) of described holding device (200).
16. reversing chamber according to claim 15 (202) configurations, comprise at least one in following features:
Comprise according at least one Further retention devices (200) in claim 1 to 14 described in any one, wherein, sample separation equipment (30) remains on different positions by means of described holding device (200) and at least one Further retention devices described (200);
Wherein, described retaining part (206) and described reversing chamber (202) are formed by this way: if sample separation equipment (30) kept by described retaining part (206) and described holding device (200) is fastened in described reversing chamber (202) time, make can not carry out between described sample separation equipment (30) and described reversing chamber (202) contacting and/or direct heat conduction connection can not being carried out;
Wherein, described reversing chamber (202) comprises can the tempering wall (210) of tempering, in described tempering wall (210), described apparatus for assembling (208) is formed for installing described fastening part (204), particularly be formed as recess, more particularly be formed as V-shape recess, T-shape recess or semi-circular indentation, be formed with outside wedge especially especially.
17. 1 kinds for the fluid sample separation in mobile phase being become the sample separation device (10) of fraction, wherein, described sample separation device (10) comprising:
Fluid delivery system, it is for carrying described mobile phase and described fluid sample;
Sample separation equipment (30), it is for becoming fraction by the described fluid sample separation in the mobile phase carried in described fluid delivery system;
According in claim 1 to 14 described in any one, for described sample separation equipment (30) being remained on the holding device (200) in the reversing chamber (202) of described sample separation device (10).
18. sample separation devices (10) according to claim 17, also comprise at least one in following characteristics:
Described sample separation equipment (30) is designed to chromatographic resolution equipment, is especially designed to chromatography column;
Described sample separation device (10) is configured at least one physics of at least one fraction to described fluid sample, chemistry and/or biological parameter and analyzes;
Described sample separation device (10) comprises by least one in the group formed for the device of chemistry, biological and/or Pharmaceutical Analysis, liquid chromatography equipment and HPLC device;
Described fluid delivery system (20) is configured for mobile phase described in high drive;
Described fluid delivery system (20) is configured for use at least 100 bar, particularly at least 500 bar, more particularly mobile phase described at least 1000 pressure-driven of clinging to;
Described sample separation device (10) is configured to microfluidic device;
Described sample separation device (10) is configured to receive fluid means;
Described sample separation device (10) comprises the detecting device (50) for detecting the fraction of described separation;
Described sample separation device (10) comprises the injector (40) for being injected into by described fluid sample in described mobile phase;
Described sample separation device (10) comprises the sample fractionator for carrying out fractionation to the fraction of described separation.
19. 1 kinds of methods for the manufacture of holding device (200), described holding device (200) is for remaining in reversing chamber (202) by sample separation equipment (30), described sample separation equipment (30) is for separating of the fluid sample in sample separation device (10), wherein, described method comprises:
Form fastening part (204), described tightening member is used for described holding device (200) to be fastened on described reversing chamber (202) place;
Form retaining part (206), described retaining part (206) is connected by this way with described fastening part (204): described retaining part (206) forms the sample separation equipment (30) for holding different size;
Described retaining part (206) is made to be formed as softer than described fastening part (204).
20. methods according to claim 19, comprise the one in following characteristics:
Wherein, described retaining part (206) by means of double teeming, the particularly formation at least partially of fastening part (204) described in injection moulding double teeming;
Wherein, described retaining part (206) and described fastening part (204) manufacture separated from one anotherly, particularly respectively by means of injection mo(u)lding separated from one another manufacture, and to be connected to each other subsequently, to be particularly connected to each other to friction lock and/or shape locking.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102014115807.5 | 2014-10-30 | ||
| DE102014115807.5A DE102014115807A1 (en) | 2014-10-30 | 2014-10-30 | Holding device for sample separation devices with sections of different strength |
Publications (2)
| Publication Number | Publication Date |
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| CN105572277A true CN105572277A (en) | 2016-05-11 |
| CN105572277B CN105572277B (en) | 2019-10-15 |
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| CN201510719519.XA Active CN105572277B (en) | 2014-10-30 | 2015-10-29 | The holding meanss of part for sample separation equipment, with varying strength |
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| Country | Link |
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| DE (1) | DE102014115807A1 (en) |
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| DE102021106086A1 (en) | 2021-03-12 | 2022-09-15 | Agilent Technologies, Inc. - A Delaware Corporation - | Temperature control of a chromatographic column |
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| DE3785207T2 (en) | 1987-09-26 | 1993-07-15 | Hewlett Packard Gmbh | PUMP DEVICE FOR DISPENSING LIQUID AT HIGH PRESSURE. |
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- 2014-10-30 DE DE102014115807.5A patent/DE102014115807A1/en active Pending
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| JPH03170863A (en) * | 1989-11-29 | 1991-07-24 | Sekisui Chem Co Ltd | Heat block for column oven of chromatograph |
| EP0545843A1 (en) * | 1991-12-03 | 1993-06-09 | Tracer Analitica S.L. | Fixing arrangement for chromatography columns |
| US20030188971A1 (en) * | 1998-02-16 | 2003-10-09 | The Institute Of Physical And Chemical Research | Capillary cassette and method of manufacturing the same |
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| CN201600354U (en) * | 2009-12-22 | 2010-10-06 | 上海悦胜芜湖药业有限公司 | Column oven |
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| Publication number | Publication date |
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| CN105572277B (en) | 2019-10-15 |
| DE102014115807A1 (en) | 2016-05-19 |
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