CN105565539A - Method for preparing nutrient phagostimulant by utilizing heparin wastewater - Google Patents
Method for preparing nutrient phagostimulant by utilizing heparin wastewater Download PDFInfo
- Publication number
- CN105565539A CN105565539A CN201510921730.XA CN201510921730A CN105565539A CN 105565539 A CN105565539 A CN 105565539A CN 201510921730 A CN201510921730 A CN 201510921730A CN 105565539 A CN105565539 A CN 105565539A
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- phagostimulant
- waste water
- heparin
- nutrition
- method utilizing
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
- C02F1/442—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis by nanofiltration
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/22—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof
Abstract
The invention discloses a method for preparing a nutrient phagostimulant by utilizing heparin wastewater. The method comprises the following steps: extracting wastewater generated by heparin by an enzymolysis method; adding a flocculant for precipitating to obtain a primary precipitate and a supernate; performing concentration by nanofiltration on the supernate; recovering and recycling the clean water which passes through a nanofiltration membrane; merging the concentrate and the primary precipitate; adding an acid to adjust the PH to be 2 to 3; performing secondary precipitating; siphoning the supernate; adding a decolorizer into the remaining precipitate for decolorizing; performing vacuum concentration on the decolorized remaining precipitate; adding amino acids and saccharides into the obtained vacuum concentrate for performing a Maillard reaction; spray-drying to obtain the phagostimulant. According to the method, water resource is recovered and utilized; the environment is purified; the economic benefit is improved; the unique stench of small intestines is improved by the prepared phagostimulant; the palatability and the food calling property of pets are enhanced; the phagostimulant is rich in nutrients, and is applied to pet staple food or pet snacks or anthony pig and chicken feed as an additive.
Description
Technical field
The present invention relates to a kind for the treatment of process of heparin waste water, particularly a kind of method utilizing heparin waste water to prepare nutrition phagostimulant.
Background technology
Current, industrial main enzymolysis process produces heparin, and raw material is pig intestinal mucosa, production technique is: fresh pig mucous membrane of small intestine adds proteolytic enzyme, under the condition of PH=8.5-9.0, temperature 50 C, NaCl salinity 3.4 ~ 5.0, and enzymolysis 2 ~ 4 hours, go out enzyme, uses 80 mesh screen, obtain filtrate.Add macropore cation-adsorption resin absorption heparin in filtrate, more after filtration, wash-out, alcohol precipitation, drying, obtain heparin crude product.
Extract in heparin technique at enzymolysis process, three process node places produce a large amount of waste water: 1. produce the rear waste water of absorption after resin absorption; 2. produce wash-out waste water after resin elution; 3. use alcohol precipitation heparin, ethanol refinery reclaims and produces the tower reactor waste water after reclaiming.This three effluent part contains the albumen after a large amount of enzymolysis, directly emits, not only can reduce the productivity effect of heparin crude product factory, also can cause environmental pollution if not treated.
The biologically active polypeptides contained in waste water has growth promoting effects, improves digestion and metabolism, adjustment internal secretion, promote the absorption of mineral substance, nervous system regulation, the multiple effect such as anti-oxidant, hypotensive and antibacterial.Current processing mode is, waste water after filtration, concentrated, spraying dry obtains intestine membrane protein, and intestine membrane protein is is easily digested and assimilated by piglet, but has the distinctive bad smell of small intestine, and purposes is restricted.
Summary of the invention
The technical problem to be solved in the present invention is: provide a kind of method utilizing heparin waste water to prepare nutrition phagostimulant, enzymolysis process is extracted the waste water reclamation that heparin process produces; The phagostimulant utilizing heparin waste water to make further, improves the distinctive bad smell of small intestine, adds in animal foodstuff.
The present invention is the technical scheme proposed that solves the problem:
1. utilize heparin waste water to prepare a method for nutrition phagostimulant, it is characterized in that comprising the following steps:
(1) extract to enzymolysis process in the waste water of heparin and add flocculation agent, precipitation, obtain throw out and supernatant liquor for the first time;
(2) supernatant liquor nanofiltration concentrates, and obtains concentrated solution and the clear water by nanofiltration membrane;
(3) merge concentrated solution and first time throw out, acid adding is adjusted to PH=2 ~ 3, carries out secondary sedimentation, siphon supernatant liquid, and residue throw out adds SYNTHETIC OPTICAL WHITNER, and bleaching final vacuum concentrates, and obtains vacuum concentration liquid;
(4) in vacuum concentration liquid, add the amino acid of its quality 0.5 ~ 2.5% and the sugar of 0.5 ~ 2%, at 60 ~ 120 DEG C, carry out Maillard reaction 0.5 ~ 2 hour;
(5) be cooled to 60 DEG C, carry out homogeneous, spraying dry after adding auxiliary material allotment, make phagostimulant.
Further, flocculation agent in described step (1) is one or more in polymerize aluminum chloride, polyaluminium sulfate and PAFS, add-on is 0.1 ~ 0.5 ‰ of wastewater quality, and cationic polyacrylamide, one in non-ionic polyacrylamide, molecular weight 500 ten thousand to 1,200 ten thousand, add-on 10 ~ 60ppm, precipitates 4 ~ 8 hours.
Further, the nanofiltration membrane molecular weight cut-off in described step (2) is 100 ~ 300 dalton.
Further, the SYNTHETIC OPTICAL WHITNER in described step (3) is sodium thiosulfate or hydrogen peroxide, and add-on is 1 ~ 2.5 ‰ of throw out quality.
Further, the supernatant volume in described step (3) accounts for and merges 2/5 of rear throw out for the first time and concentrated solution cumulative volume, and the total solid content of described vacuum concentration liquid is 30 ~ 50%.
Further, the amino acid in described step (4) is selected from one or more in halfcystine, methionine(Met), glycine and L-Ala, sugar be selected from wood sugar, glucose one or both.
Further, in described step (5) auxiliary material be meat flavour, and be selected from the one in W-Gum, dextrin, supplementary product consumption is 1 ~ 5% of vacuum concentration liquid quality.
Utilize nutrition phagostimulant prepared by heparin waste water, as additive application in the raising of pet staple food or pet treat or sucking pig, chick, improve the food ration of animal.
Beneficial effect of the present invention: when supernatant liquor nanofiltration concentrates, is recycled by the clear water of nanofiltration membrane, has purified environment, has increased economic efficiency; The phagostimulant made improves the distinctive bad smell of small intestine, enhances the palatability and attractant of raising animalcule, nutritious, is the staple food of pet (comprising sucking pig, chick) and processing raw material of snacks; The waste water that phagostimulant utilizes heparin to produce is made, utilization of waste material, and cost of manufacture is cheap, solve prior art adopt pure natural plant extract and animal livers extract etc. be raw material, expensive, production cost high shortcoming.
Embodiment
Embodiment:
The waste water that Zymolysis process of producing heparin sodium produces is 5000 liters, the polyacrylamide adding 6,000,000 molecular weight of 10 kilograms of polymerize aluminum chlorides and 50ppm stirs, and precipitate after 5 hours, throw out accounts for 1/4 of total liquid volume, that is: 1250 liters, the volume of supernatant liquor is 3750 liters.Supernatant liquor concentrates through nanofiltration membrane nanofiltration, and the molecular weight cut-off of nanofiltration membrane is 100 dalton, by 3000 premium on currency recycling uses of nanofiltration membrane, and the volume 750 liters of residue concentrated solution.Merge primary sedimentation thing and concentrated solution, obtain 2000 liters, PH=2 is regulated with sulfuric acid, staticly settle 4 hours, the clear liquid on upper strata is 800 liters, nanofiltration again after siphon, residue secondary precipitate 1200 liters adds 2.5 kilograms, hydrogen peroxide, bleach 4 hours, with economic benefits and social benefits falling film type thickener vacuum-concentrcted, obtain the concentrated solution that total solid content is 50%.
Get the concentrated solution of 200 grams of solid content 50%, add wood sugar 0.5g, glycine 0.05g, Cys2 g, 60 DEG C are reacted 2 hours.After reaction terminates, add 2g maltodextrin at about 60 DEG C, 0.2g meat flavour, allocates, and mixes and carries out spraying dry and obtain phagostimulant.
Phagostimulant character analysis: fragrance is based on meat flavour, and fishy smell is lighter, with cheese taste, fat taste is denseer, fragrance coordinate and stronger, stingless excitation.
Test example: the phagostimulant 500g got according to embodiment is obtained carries out animal feeding trials.
Experimental animal: the Poodle 10 selecting 1 years old, tests.
Diet: prepare voluntarily according to NRC standard poultry is main, diet recipe is as following table.
In test, test group adds phagostimulant ratio is 0.5%, allows animal unrestricted choice search for food different diet, 15 days experimental periods.Found that, the search for food autogamy dog flow vector that is added with phagostimulant of laboratory animal is 2.12 times of control group, and the laboratory animal of the autogamy dog grain being added with phagostimulant of directly searching for food after experiment in 15 days accounts for 80% of sum.The visible phagostimulant made can play good food calling effect, enhances palatability and the attractant of pet.
Claims (8)
1. utilize heparin waste water to prepare a method for nutrition phagostimulant, it is characterized in that comprising the following steps:
(1) extract to enzymolysis process in the waste water of heparin and add flocculation agent, precipitation, obtain throw out and supernatant liquor for the first time;
(2) supernatant liquor nanofiltration concentrates, and obtains concentrated solution and the clear water by nanofiltration membrane;
(3) merge concentrated solution and first time throw out, acid adding is adjusted to PH=2 ~ 3, carries out secondary sedimentation, siphon supernatant liquid, and residue throw out adds SYNTHETIC OPTICAL WHITNER, and bleaching final vacuum concentrates, and obtains vacuum concentration liquid;
(4) in vacuum concentration liquid, add the amino acid of its quality 0.5 ~ 2.5% and the sugar of 0.5 ~ 2%, at 60 ~ 120 DEG C, carry out Maillard reaction 0.5 ~ 2 hour;
(5) be cooled to 60 DEG C, carry out homogeneous, spraying dry after adding auxiliary material allotment, make phagostimulant.
2. a kind of method utilizing heparin waste water to prepare nutrition phagostimulant according to claim 1, it is characterized in that, flocculation agent in described step (1) is one or more in polymerize aluminum chloride, polyaluminium sulfate and PAFS, add-on is 0.1 ~ 0.5 ‰ of wastewater quality, and cationic polyacrylamide, one in non-ionic polyacrylamide, molecular weight 500 ten thousand to 1,200 ten thousand, add-on 10 ~ 60ppm, precipitates 4 ~ 8 hours.
3. a kind of method utilizing heparin waste water to prepare phagostimulant according to claim 1, it is characterized in that, the nanofiltration membrane molecular weight cut-off in described step (2) is 100 ~ 300 dalton.
4. a kind of method utilizing heparin waste water to prepare nutrition phagostimulant according to claim 1, it is characterized in that, the SYNTHETIC OPTICAL WHITNER in described step (3) is sodium thiosulfate or hydrogen peroxide, and add-on is 1 ~ 2.5 ‰ of throw out quality.
5. a kind of method utilizing heparin waste water to prepare nutrition phagostimulant according to claim 1, it is characterized in that, supernatant volume in described step (3) accounts for and merges 2/5 of rear throw out for the first time and concentrated solution cumulative volume, and the total solid content of described vacuum concentration liquid is 30 ~ 50%.
6. a kind of method utilizing heparin waste water to prepare nutrition phagostimulant according to claim 1, it is characterized in that, amino acid in described step (4) is selected from one or more in halfcystine, methionine(Met), glycine and L-Ala, sugar be selected from wood sugar, glucose one or both.
7. a kind of method utilizing heparin waste water to prepare nutrition phagostimulant according to claim 1, it is characterized in that, in described step (5) auxiliary material be meat flavour, and be selected from the one in W-Gum, dextrin, supplementary product consumption is 1 ~ 5% of vacuum concentration liquid quality.
8. a nutrition phagostimulant, is characterized in that, is made up of the method utilizing heparin waste water to prepare nutrition phagostimulant a kind of described in claim 1, as additive application in the raising of pet staple food or pet treat or sucking pig, chick.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510921730.XA CN105565539B (en) | 2015-12-14 | 2015-12-14 | A method of preparing nutrition phagostimulant using heparin waste water |
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| CN201510921730.XA CN105565539B (en) | 2015-12-14 | 2015-12-14 | A method of preparing nutrition phagostimulant using heparin waste water |
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| CN105565539A true CN105565539A (en) | 2016-05-11 |
| CN105565539B CN105565539B (en) | 2018-10-26 |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102366026A (en) * | 2011-09-30 | 2012-03-07 | 徐州联益生物科技开发有限公司 | Method for making pet food attractant |
| CN102617756A (en) * | 2012-03-23 | 2012-08-01 | 武汉博锐通科技有限公司 | Preparation method of heparin lithium |
| CN103626836A (en) * | 2012-08-21 | 2014-03-12 | 丰都泓乾生物科技有限公司 | Extraction process for polypeptide from waste liquid obtained in production of crude heparin sodium product |
| WO2014041118A1 (en) * | 2012-09-13 | 2014-03-20 | Specialites Pet Food | Use of fat compositions for sustaining an enhanced palatability of pet food over time |
| CN103923164A (en) * | 2014-04-21 | 2014-07-16 | 合肥工业大学 | Resourceful treatment method of casing-heparin processing wastewater |
| CN104171260A (en) * | 2013-07-31 | 2014-12-03 | 北京澳龙港生物技术研究中心 | Functional peptide protein powder, and preparation method and applications thereof |
| CN104829750A (en) * | 2015-05-26 | 2015-08-12 | 苏州鸿洋医药科技有限公司 | Refining process of heparin lithium |
-
2015
- 2015-12-14 CN CN201510921730.XA patent/CN105565539B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102366026A (en) * | 2011-09-30 | 2012-03-07 | 徐州联益生物科技开发有限公司 | Method for making pet food attractant |
| CN102617756A (en) * | 2012-03-23 | 2012-08-01 | 武汉博锐通科技有限公司 | Preparation method of heparin lithium |
| CN103626836A (en) * | 2012-08-21 | 2014-03-12 | 丰都泓乾生物科技有限公司 | Extraction process for polypeptide from waste liquid obtained in production of crude heparin sodium product |
| WO2014041118A1 (en) * | 2012-09-13 | 2014-03-20 | Specialites Pet Food | Use of fat compositions for sustaining an enhanced palatability of pet food over time |
| CN104171260A (en) * | 2013-07-31 | 2014-12-03 | 北京澳龙港生物技术研究中心 | Functional peptide protein powder, and preparation method and applications thereof |
| CN103923164A (en) * | 2014-04-21 | 2014-07-16 | 合肥工业大学 | Resourceful treatment method of casing-heparin processing wastewater |
| CN104829750A (en) * | 2015-05-26 | 2015-08-12 | 苏州鸿洋医药科技有限公司 | Refining process of heparin lithium |
Non-Patent Citations (2)
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| 舒翔等: "肠衣生产废水多肽的絮凝回收", 《饲料工业》 * |
| 许春龙等: "利用肠衣-肝素加工废水生产饲料蛋白粉的资源化利用初探", 《畜牧与饲料科学》 * |
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| CN105565539B (en) | 2018-10-26 |
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