KR20000072189A - Fish extract using waste matter of fishes, method for preparing the same and livestock feed containing the same - Google Patents

Abstract

PURPOSE: Method for preparing extract of fish meat using by-products of fish is provided to supply stock feed containing chitosan, DHA, and EPA by reusing waste. CONSTITUTION: Method for preparing extract of fish meat using by-products of fish comprises the following steps:(i) a first step to add 100-2000wt.% of water to 100wt.% of by-products of fish, and dissolve the flesh and bone of fish under a condition of 100°C or more and 1.1 atmospheric pressure or more for 2-4hours, or a condition of 60-105°C and normal pressure for 1-5 hours; (ii) a second step to add 10-200ml of chitosan whose deacetylation degree is more than 95%, and viscosity is more than 12,000cps, and make it cohesion-precipitated to separate solidified matter and solution; and (iii) a third step to filter the matter, dewater, and dry. The prepared extract of fish flesh can be contained in a feed in amount of 0.1-5.0wt.%.

Description

Fish extract using waste matter of fishes, method for preparing the same and livestock feed containing the same}

The present invention relates to a fish meat extract using fish by-products, a method for preparing the same and a livestock feed containing the same, and more specifically, by-products and boiled by-products such as fish heads, intestines, etc. generated during canned fish, precipitated by chitosan The present invention relates to a fish meat extract containing chitosan and containing useful components such as DHA and EPA of high protein, a method of preparing the same, and a livestock feed containing the same.

Domestic fish processing plants (tuna, saury, mackerel, pollock, squid, cutlass, etc.) are distributed throughout the country, and by-products of fish (e.g. head, bone, tail, intestines, Epidermis, etc.) is used as part of the feed, and the surplus is mostly discarded as waste. In particular, by-products such as fish heads and intestines generated from the canned fish factory are omega-3 fatty acids and useful components such as DHA (Dehydroepiandrosterone) and EPA (Eicisapentaenoic acid), which are known to be effective in preventing intelligent development and senile dementia. It contains a lot of, and a large amount of about 15 tons per day on a dry content basis, but most of the situation is discarded.

Looking at the example of manufacturing a feed using fish by-products in the prior art, Korean Patent Laid-Open Publication No. 99-46663 discloses a method for producing a juice adsorption fermented feed, and Korean Patent Publication No. 83-8742 discloses a fish juice adsorption feed There is a manufacturing method, Korean Patent Laid-Open No. 93-1801 discloses a manufacturing method of a high protein high calcium feed using fish residues. However, the above methods are concentrated by-products of fish, and then feed pellets are added to prepare a livestock feed in the form of pellets, and the excess water is used to separate the supernatant (fish meat) and sediment (fish meat extract) There is no situation.

On the other hand, chitosan is widely used in medical and health foods, and the use of livestock feed additives has been used for the purpose of replacing antibiotics and improving meat quality of livestock. Related patents are Korean Registered Patent Nos. 157366, 22132, 222133, etc., mainly to improve broiler meat by adding 0.1 to 0.3% by weight of chitosan and adding fine sand to broiler feed. In addition, Korean Patent Publication No. 99-9390 discloses healthy soy sauce containing chitosan and minerals, and Korean Patent No. 43491 discloses an example of using a chitosan-based copolymer flocculant for wastewater treatment. In 93-12593, chitosan was used to directly remove heavy metals from leather wastewater. However, there is no example that chitosan was used in the preparation of fish meat extract.

Therefore, in the present invention, it was found that the fish meat extract containing chitosan can be obtained from the solids by distilling or partially drying the canned by-products of canned fish which are used as feed ingredients and then boiling them. The invention has been completed based on this. As described above, in the present invention, by reusing fish by-products, the environment-friendly clean technology can be developed to solve the problem of shortage of landfills, and the load of incinerators can be reduced to solve the environmental problems that have emerged as problems around the world in the 21st century. .

Accordingly, it is an object of the present invention to provide a method of preparing fish meat extract using fish by-products.

Another object of the present invention to provide a fish meat extract prepared by the above method.

Another object of the present invention to provide a livestock feed containing the fish meat extract.

Fish meat extract using the fish by-product of the present invention for achieving the above object is 100 to 2,000 parts by weight of water with respect to 100 parts by weight of fish by-products hot and high pressure (100 ℃ or more and 1.1 atm or more), or high temperature ( 60 to 105 ° C.) and melting the meat and bone of the fish at atmospheric pressure; Chitosan is added to the melt to coagulate and precipitate the solids and the solution portion; And filtering, dehydrating and drying the solid.

Fish meat extract of the present invention for achieving the above another object is prepared by the above method, and contains chitosan, DHA and EPA.

Livestock feed of the present invention for achieving the above another object includes the fish meat extract.

1 is a block diagram schematically showing a process for preparing a fish meat extract in accordance with the present invention.

Looking at the present invention in more detail as follows.

High energy (energy including the time used for the formation of substances by synthesizing elements such as carbon, hydrogen, oxygen, sulfur, nitrogen, etc. by living organisms) If landfills or incineration of fish are buried or incinerated, It is broken down. Therefore, this energy (organic materials (proteins, fats, carbohydrates), minerals, and landfill and incineration costs used in synthesizing the functional substances contained in the fish) is discarded for a long time. By converting this high energy back into energy that can be used by humans, it leads to energy protection and energy saving.

In the present invention, by adding an excessive amount of water to the by-product of the fish and exposed to a high temperature atmospheric pressure (60 ~ 105 ℃) state or high temperature high pressure (100 ℃ or more, 1.1 atm or more) state to decompose the meat and bones of the fish, and aggregated with chitosan To obtain a fish meat extract containing a high functional substance. As such, one method of sterilizing pathogenic microorganisms is to expose fish byproducts to high temperatures so that animals can use them for food. As described above, the present invention adds excess water to fish by-products, thereby maximally decomposing meat and bones of fish under high temperature and high pressure, and decomposing solids as much as possible by using chitosan known as a functional substance. It is possible to obtain a supernatant and a solid containing a substance. For reference, the supernatant may be used as an additive of a functional food such as stem steel.

In the present invention, as a result of studying the amount of water, temperature and pressure necessary to decompose and process fish by-products can be maintained optimally, most meat melted by boiling for 1 to 5 hours at atmospheric pressure. In addition, in the high temperature and high pressure state, most meat quality melted by processing for 2 to 4 hours. The amount of water to be added is preferably 100 to 2,000 parts by weight based on 100 parts by weight of fish by-products. If the amount is less than 100 parts by weight, the amount of solids is increased. You have to do it twice.

In the present invention, a chemical flocculation precipitation technique was used to separate the processed fish by-product into solid and solution fractions. In the chemical flocculation technique, chitosan, which is a cationic natural polymer polysaccharide, is used, and an optimal chitosan was selected according to the chemical properties of chitosan and used for agglomeration separation of the extract.

In the present invention, it was tested according to the amount of water added to the amount of product, and the amount of chitosan added after processing at high temperature in order to extract the functional substance from the fish by-product. The results are shown in Tables 1 and 2 below.

Amount of sediment according to the amount of water added during fish decomposition processing sample Amount of water / fish byproduct (kg / kg) Sediment Dry Weight / Fish byproduct (kg / kg) One 1/1 0.85 2 3/1 0.77 3 5/1 0.74 4 10/1 0.75 5 20/1 0.70

The amount of sediment according to the amount of chitosan added during fish decomposition processing sample Addition amount of 1% chitosan solution (ml / kg) Sediment Dry Weight / fish byproduct (g / g) One 10 0.35 2 30 0.56 3 50 0.86 4 100 0.87 5 150 0.86 6 200 0.88

The selection of chitosan used in the present invention was tested according to the degree of deacetylation and viscosity, and the higher the viscosity and the degree of deacetylation, the more economical. The optimal chitosan was at least 95% deacetylation and at least 12000 cps in viscosity (1% solution). In addition, the amount of chitosan added is preferably 10 to 200 ml of 1% chitosan per 1 kg of fish byproduct. If less than 10 ml, precipitation does not occur, and if more than 200 ml, more than necessary chitosan is added.

On the other hand, as shown in Figure 1, in the present invention by filtering the precipitate (solid content) obtained by extracting the fish by-product to obtain a fish meat extract. These fish extracts contain large amounts of chitosan, DHA and EPA. The fish meat extract can be used directly as a livestock feed, it can also be added to existing livestock feed such as compound feed. The amount added at this time is determined according to the type of animal to be fed and the ingredients and price of grains and fish meal used in the feed, and about 0.1 to 5.0% by weight of the feed for pigs, chickens and cattle is added to the productivity and economics of the livestock. It is preferable at the point of phosphorus. The feed of the present invention contains 30 to 50 parts by weight of protein, 0.7 to 3.0 parts by weight of chitosan, and a large amount of minerals essential for livestock such as calcium and phosphorus, and contains DHA and EPA.

Hereinafter, the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the following examples.

Example 1

2 kg of water was added to 1 kg of fish by-product and heated at 110 ° C. and 1.1 atm for about 3 hours to melt the meat and bone of the fish. 200 ml of 1% aqueous chitosan solution was added to the melt to separate the solids and the solution portion. The amount of solids at this time was 0.88 kg, and the supernatant was 2.32 kg. The solids were filtered, dehydrated and dried to obtain a fish meat extract of the present invention.

DHA, EPA and the composition of the fish meat extract obtained by the method of Example 1 are shown in Table 3 below. DHA and EPA were analyzed by High Performance Liquid Chromatography (HPLC) using DHA and EPA purchased from Sigma as standard materials, and general ingredient analysis was carried out according to the Feed Analysis Report (Feed Analysis, 1986, Advanced Culture History). Analyzed. As shown in Table 3, the content of DHA and EPA was high.

Component Analysis Results of the Prepared Samples Item / Component DHA EPA moisture Ash Crude protein Crude fat Crude fiber Chitosan Manufacture Sample 5367 ug / g 3764 ug / g 5.97% 19.56% 34.60% 36.31% 0% 1.0%

In addition, the results of analyzing the harmful ingredients to investigate the safety of the feed by feed the fish meat extract prepared in Example 1 are shown in Table 4. The comparative limits are those for animal protein feeds, including fishmeal, in the range and criteria for harmful feeds specified in Article 12 of the Enforcement Decree of the Feed Control Act.

Hazardous Substance Analysis Results for Safety Confirmation of Prepared Samples Hazardous Substance Name Analytical Content Acceptance criteria chrome 20 ppm 100 ppm lead 5 ppm 10 ppm Mercury 0.1 ppm 0.5 ppm cadmium 1.0 ppm 2.5 ppm

As a result of the analysis, the livestock feed according to the present invention can be used as a safe feedstock suitable for feed acceptance criteria.

As described above, the present invention not only replaces fish meal, which is a raw material dependent on imports, but also contains chitosan to prevent disease of livestock, thereby reducing the use of antibiotics, and developing brains of humans such as DHA and EPA. The useful ingredients in the livestock products accumulate in the livestock products, and the effect of promoting the brain development of those who consume these livestock products can be obtained. In particular, it is of great significance to protect the environment by converting waste into high-quality livestock feed.

Claims (4)

Melting 100 to 2,000 parts by weight of water with respect to 100 parts by weight of fish by-product to melt meat and bone of fish at high temperature and high pressure (100 ° C. or higher and 1.1 atm or higher), or high temperature (60 to 105 ° C.) and atmospheric pressure; Deacetylation degree in the melt is more than 95%, the viscosity of more than 12,000cps chitosan by adding 1% chitosan 10 ~ 200ml to 1kg fish by-products by flocculation and precipitation to separate the solids and the solution portion; And Filtration, dehydration and drying the solid content, characterized in that the method for producing a fish meat extract using fish by-products comprising the step of drying. The method of claim 1, wherein the melting time is maintained for 2 to 4 hours under the high temperature and high pressure conditions, and the melting time is 1 to 5 hours under the high temperature and atmospheric pressure conditions. A fish meat extract containing chitosan, DHA and EPA prepared by the method of any one of claims 1 to 2. Animal feed comprising 0.1 to 5.0% by weight of the fish meat extract according to claim 3.