CN105558896A - Composition containing effective components of sea cucumber and cordyceps sinensis, preparation method and application thereof - Google Patents
Composition containing effective components of sea cucumber and cordyceps sinensis, preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention relates to a composition containing effective components of sea cucumber and cordyceps sinensis, a preparation method and application thereof, and belongs to the field of health products. The composition comprises sea cucumber extract and cordyceps sinensis extract. The composition has the advantages that the formula is unique, the preparation method is simple, and the composition can be made into sea cucumber and cordyceps sinensis liquor, sea cucumber and cordyceps sinensis beverage, and sea cucumber and cordyceps sinensis oral liquid, which are user-friendly and have prominent healthcare functions. The sea cucumber and cordyceps sinensis are taken as the main raw materials and combined together, the warm property and cold property are complementary, and thus the composition is suitable for more people. The formula and technology are scientific, the ratio of effective components in sea cucumber and cordyceps sinensis is effective increased, the nutrients can be fully utilized; the immunity is improved, the aging is retarded, the physique is enhanced, and the life quality is improved. Moreover, the smell of sea cucumber is removed, the smell removing and filtering treatment of sea cucumber is not needed, the nutrient loss of sea cucumber is avoided therefore, the amount of cordyceps sinensis extract is calculated by cordycepin, the content of effective components of cordyceps sinensis can be explicitly expressed, and better healthcare functions can be reached.
Description
Technical field
The invention belongs to daily health caring food, particularly a kind of composition and method of making the same and application containing sea cucumber and Chinese caterpillar fungus composition.
Background technology
Along with modern society's rhythm of life is accelerated, operating pressure is large, amount of exercise reduces, and people's physique starts to decline.The crowd that hypoimmunity suffers from inferior health increases sharply, and the crowd suffering from the illnesss such as hypertension, high fat of blood, hyperglycaemia, artery sclerosis, coronary heart disease, cancer becomes younger seriously.Therefore, people more and more recognize the importance improving immunity, delay senility, build up health, improve the quality of living.
Sea cucumber is integration of drinking and medicinal herbs, widely known invigorant.Record according in " five assorted ancient sacrificial utensils ": " sea cucumber, beach, the east area of the Liao River, coextensive with eastern and southern Liaoning Province has it, its benefit warm in nature, sufficient enemy's ginseng, therefore named sea cucumber ".Sea cucumber is the animality health products of high nutrition, it is containing acid mucopolysaccharide, higher protein (being especially rich in collagen), 18 seed amino acids and various trace elements, having and delay senility, blood-enriching face-nourishing, raising immunity, the anti-inflammatory that cures the wound, protect liver and protect the effect such as blood vessel, brain healthy, inhibition cancer cell, is the nutrient excellent product of modern.Traditional beche-de-mer products has salted sea cucumber, half dried sea-cucumber, freeze-drying sea cucumber and instant holothurian etc., and there is the problem that nutrition absorption rate is low, people develop sea cucumber polypeptide for this reason.The extracting method of sea cucumber peptide in the prior art mostly by embathing, autoclaving, enzymolysis, de-taste, filter, concentrate, dry etc. operation makes.Although by de-tasting operation, the sea cucumber peptide obtained by prior art still has fishy smell, take separately be of low nutritive value, mouthfeel is poor.
Chinese caterpillar fungus is the famous and precious tonic herb of China's usual one among the people, and people are usually classified as it and ginseng, pilose antler as three large tonics, expensive, are described as " soft gold " by people.Particularly Cordyceps militaris, wherein containing a large amount of cordycepins.The special medical health care of cordycepin has caused the great attention of domestic and international expert, and existing many health products based on cordycepin, health food, cosmetics, medicine are put on market.Form mainly contains cordyceps sinensis medicine liqueur, cordyceps medicinal food, cordyceps tea, cordyceps drink and all kinds of capsule, tablet etc., but existing worm grass product does not all have the content of clear and definite wherein cordycepin, cause a lot of consumer to have purchased worm grass product without cordycepin.
Up to now, not based on the health products of sea cucumber peptide and cordycepin for improving health of people, raising immunity, the relevant report of sea cucumber worm grass product that delays senility.
Summary of the invention
For improving immunity, delay senility, build up health, improve the quality of living, the present invention develops the health food containing sea cucumber and Chinese caterpillar fungus composition.The present invention is achieved by the following technical solutions:
Contain a composition for sea cucumber and Chinese caterpillar fungus active ingredient, the composition of described composition comprises: sea cucumber extract and Chinese caterpillar fungus extract.
Under preferred embodiment, described composition contains sea cucumber extract 0.2%-1.6% (w/v), Chinese caterpillar fungus extract in cordycepin content for 0.002%-0.032% (w/v).
The present invention also protects the method preparing above-mentioned composition, comprises the following step: S1 prepares sea cucumber extract; S2 prepares Chinese caterpillar fungus extract; The Product mix that S1 and S2 is prepared by S3 evenly makes composition.
Under preferred embodiment, the concrete steps of described S1 are: sea cucumber gills by S1.1 cleaning, cleans silt; Clean sea cucumber is placed in hydrolytic decomposition pot by S1.2 pretreatment, adds water; Step S1.2 product is made after sea cucumber milk high-pressure homogeneous through beater by S1.3 slurrying; S1.4 enzymolysis adds the protease hydrolyzed of 0.3-0.5% (v/v) in the obtained product of S1.3, and go out thereafter enzyme; S1.5 separation and Extraction is by enzymolysis liquid centrifugal filtration, and it is for subsequent use to get filtrate; Filtrate is concentrated by vacuum concentration pot by S1.6 reduced pressure concentration, and the concentrate of acquisition is sea cucumber extract.
The concrete steps of described S2 are: it is dry in microwave vacuum dryer that S2.1 drying and crushing gets Chinese caterpillar fungus, after pulverizer is pulverized, obtains Chinese caterpillar fungus powder; S2.2 is concentrated is fully dissolved in water by step S2.1 product, filters, gets filtrate and concentrate in vacuum concentration pot, obtain Chinese caterpillar fungus extract after lixiviate.
Under further preferred embodiment, preparation process S2 also comprises: S2.3 determines the content of cordycepin; Described step S2.3 adopts ultraviolet spectrophotometry.
The present invention also protects sea cucumber and Chinese caterpillar fungus composition preparing the application in health products or food.
The present invention also protects a kind of health products or food, wherein contains sea cucumber Chinese caterpillar fungus composition as active ingredient; Described health products or food are oral formulations.
Under preferred embodiment, described health products or food are oral liquid, medicinal liquor and beverage.
Advantage of the present invention and good effect are:
1, the present invention fills a prescription uniqueness, and preparation method is simple, and obtained sea cucumber cordyceps militaris liquor, sea cucumber cordyceps drink and sea cucumber cordyceps militaris oral liquid, instant, health care is remarkable.
2, the present invention with sea cucumber and Chinese caterpillar fungus for primary raw material: sea cucumber belongs to micro-cool property, and Chinese caterpillar fungus belongs to warm nature, and both combine, and warm cool complementation, complement each other, suitable population is more extensive.
3, formula for a product of the present invention and preparation technology's science, effectively promotes sea cucumber, the original functional component amount of Chinese caterpillar fungus than level, realizes nutraceutical Optimum utilization, can improve immunity, delay senility, build up health, improve the quality of living.
4, reasonably component effectively can remove the fishy smell of sea cucumber, without the need to carrying out de-raw meat filtration treatment to sea cucumber, avoids sea cucumber nutrient ingredients from lossing.
5, the present invention adopts enzymolysis process to prepare sea cucumber peptide, and is improved the enzymolysis process of sea cucumber.By measuring the content of sea cucumber peptide, enzymatic hydrolysis condition being groped repeatedly, finally determines enzymolysis process.Enzymolysis process after improvement significantly improves the bioavilability of sea cucumber, fully remains the main nutrient composition of sea cucumber.
6, formula for a product Chinese caterpillar fungus extract of the present invention measures with cordycepin, clearly can indicate the content of Chinese caterpillar fungus active ingredient, reaches better above-mentioned health care.
Accompanying drawing explanation
Fig. 1 is preparation is the process chart of the composition of active ingredient containing sea cucumber and Chinese caterpillar fungus.
Detailed description of the invention
Below by specific embodiment, the invention will be further described.Following examples are descriptive, are not determinate, can not limit protection scope of the present invention with this.Experimental technique described in embodiment if no special instructions, is conventional method; If no special instructions, described reagent and biomaterial, all can obtain from commercial channels.In following examples, neutral compound protease is buied from Wuxi City enzyme preparation factory or Dong Henghua road, Nanning biotechnology Co., Ltd, also can with pepsin thing as an alternative.In following examples, Chinese caterpillar fungus content is all with the cubage of cordycepin.
Embodiment 1
The preparation of sea cucumber extract
Cleaning: gilled by chilled sea cucumber, cleans silt.
Pretreatment: be placed in hydrolytic decomposition pot by clean sea cucumber, adds 1-2 times of deionized water of quality of sea cucumber.
Slurrying: above-mentioned sea cucumber is made slurry from solution economy-combat pulp grinder, more high-pressure homogeneous under 20-25 kilogram of pressure.
Enzymolysis: add neutral compound protease by the volume ratio of 0.3-0.5%, enzymolysis 4h, go out thereafter enzyme 20min.
Separation and Extraction: by centrifugal for enzymolysis liquid 20min, filters, gets filtrate, for subsequent use.
Reduced pressure concentration: filtered fluid is concentrated by vacuum concentration pot, the concentrate of acquisition is sea cucumber extract.
Embodiment 2
The content of cordycepin in wild Chinese caterpillar fungus is atomic, and the present invention selects the high-quality Cordyceps militaris of the special high cordycepin content cultivated to be raw material, preparation Chinese caterpillar fungus extract.
Chinese caterpillar fungus powder: Cordyceps militaris with dry 1h at 50-55 DEG C, was pulverized after 100-120 mesh sieve through pulverizer in microwave vacuum dryer, obtains Chinese caterpillar fungus powder.
Chinese caterpillar fungus extract: adding is the water of Chinese caterpillar fungus opaque amount 6 times, and lixiviate 4h, after filtration, concentrates in vacuum concentration pot, obtains Chinese caterpillar fungus extract.
Determine the content of cordycepin in Chinese caterpillar fungus extract: precision takes 1mL cordycepin reference substance, dissolve with ultra-pure water, dilution is settled to 10ml, makes 0.1mgL
-1mother solution.Mother liquor is diluted certain multiple, ultraviolet specrophotometer measures.Mensuration wavelength is 200-400nm.Obtain absorbency Y, with absorbency Y to cordycepin mass concentration (mgmL
-1) X does calibration curve.Get the Chinese caterpillar fungus extract 1 part dilution of preparation, survey light absorption value at 1,2,4,8,12h respectively at maximum absorption wavelength place.The content of cordycepin in Chinese caterpillar fungus extract is calculated, according to the data calculated, according to the content of the ratio-dependent cordycepin of Chinese caterpillar fungus active ingredient by calibration curve.In addition to the above methods, detection method also has high performance liquid chromatography.
Embodiment 3
Sea cucumber cordyceps militaris liquor comprises following material, and sea cucumber extract and Chinese caterpillar fungus extract obtain through the preparation method of embodiment 1 and embodiment 2, and the mass percent of its each component is as follows:
Optimum, the consumption of each component of sea cucumber cordyceps militaris liquor content is: white wine 50-70%, sea cucumber extract 0.4%, Chinese caterpillar fungus extract 0.006%, and all the other are water.
Embodiment 4
Sea cucumber cordyceps militaris oral liquid comprises following material, and sea cucumber extract and Chinese caterpillar fungus extract obtain through the preparation method of embodiment 1 and embodiment 2, and the mass percent of its each component is as follows:
Optimum, the consumption of each component of sea cucumber cordyceps drink content is: sea cucumber extract 1.6%, Chinese caterpillar fungus extract 0.002%, STEVIA REBAUDIANA 0.3%, ethyl maltol 0.03%, sodium carboxymethylcellulose 0.15%, citric acid 0.15%, white granulated sugar 2%, and all the other are water.
Optimum, the consumption of each component of sea cucumber cordyceps militaris oral liquid content is: sea cucumber extract 1.6%, Chinese caterpillar fungus extract 0.032%, STEVIA REBAUDIANA 0.3%, ethyl maltol 0.03%, sodium carboxymethylcellulose 0.15%, citric acid 0.15%, white granulated sugar 2%, and all the other are water.
The sea cucumber extract adopted in embodiment 5-13 is by the obtained product of embodiment 1, and the Chinese caterpillar fungus extract of employing is by the obtained product of embodiment 2.Embodiment 5-13 composite formula is only the example of embodiment 3 or embodiment 4, can not be used for limiting the scope of embodiment 3 or embodiment 4.
Embodiment 5
Get white wine, sea cucumber extract, Chinese caterpillar fungus extract, by mass percentage: white wine 50%, sea cucumber extract 0.2%, Chinese caterpillar fungus extract 0.003%, all the other are water.Raw material stirs after 1h through 25-35r/min, allocates by each component, in diatomite coarse filtration under 2.5 kilograms of high pressure, after the filter of 1.5 kilograms of health liquor filter essences, makes sea cucumber cordyceps militaris liquor.The alcoholic strength of sea cucumber cordyceps militaris liquor of the present invention is 20 °-40 °.
Embodiment 6
Get white wine, sea cucumber extract, Chinese caterpillar fungus extract, by mass percentage: white wine 60%, sea cucumber extract 0.3%, Chinese caterpillar fungus extract 0.004%, all the other are water.Raw material stirs after 1h through 25-35r/min, allocates by each component, in diatomite coarse filtration under 2.5 kilograms of high pressure, after the filter of 1.5 kilograms of health liquor filter essences, makes sea cucumber cordyceps militaris liquor.The alcoholic strength of sea cucumber cordyceps militaris liquor of the present invention is 20 °-50 °.
Embodiment 7
Get white wine, sea cucumber extract, Chinese caterpillar fungus extract, by mass percentage: white wine 70%, sea cucumber extract 0.4%, Chinese caterpillar fungus extract 0.006%, all the other are water.Raw material stirs after 1h through 25-35r/min, allocates by each component, in diatomite coarse filtration under 2.5 kilograms of high pressure, after the filter of 1.5 kilograms of health liquor filter essences, makes sea cucumber cordyceps militaris liquor.The alcoholic strength of sea cucumber cordyceps militaris liquor of the present invention is 20 °-65 °.
Embodiment 8
Get sea cucumber extract, Chinese caterpillar fungus extract, STEVIA REBAUDIANA, ethyl maltol, sodium carboxymethylcellulose, citric acid, white granulated sugar, by mass percentage: sea cucumber extract 1.0%, Chinese caterpillar fungus extract 0.002%, STEVIA REBAUDIANA 0.2%, ethyl maltol 0.02%, sodium carboxymethylcellulose 0.08%, citric acid 0.08%, white granulated sugar 1%, all the other are water.Prepare by each component, packing after twice high-pressure homogeneous.First time is high-pressure homogeneously: 170-180 kilogram high-pressure homogeneous; Second time high-pressure homogeneous be 250-280 kilogram high-pressure homogeneous.Adopt the instantaneous sterilizing method sterilizing of 115 DEG C of sterilizing 5s, product is tested, is up to the standards and packs, make sea cucumber cordyceps drink.
Embodiment 9
Get sea cucumber extract, Chinese caterpillar fungus extract, STEVIA REBAUDIANA, ethyl maltol, sodium carboxymethylcellulose, citric acid, white granulated sugar, by mass percentage: sea cucumber extract 1.5%, Chinese caterpillar fungus extract 0.004%, STEVIA REBAUDIANA 0.25%, ethyl maltol 0.03%, sodium carboxymethylcellulose 0.1%, citric acid 0.1%, white granulated sugar 1.5%, all the other are water.Prepare by each component, packing after twice high-pressure homogeneous.First time is high-pressure homogeneously: 170-180 kilogram high-pressure homogeneous; Second time high-pressure homogeneous be 250-280 kilogram high-pressure homogeneous.Adopt the instantaneous sterilizing method sterilizing of 115 DEG C of sterilizing 5s, product is tested, is up to the standards and packs, make sea cucumber cordyceps drink.
Embodiment 10
Get sea cucumber extract, Chinese caterpillar fungus extract, STEVIA REBAUDIANA, ethyl maltol, sodium carboxymethylcellulose, citric acid, white granulated sugar, by mass percentage: sea cucumber extract 1.6%, Chinese caterpillar fungus extract 0.01%, STEVIA REBAUDIANA 0.3%, ethyl maltol 0.03%, sodium carboxymethylcellulose 0.15%, citric acid 0.15%, white granulated sugar 2%, all the other are water.Prepare by each component, packing after twice high-pressure homogeneous.First time is high-pressure homogeneously: 170-180 kilogram high-pressure homogeneous; Second time high-pressure homogeneous be 250-280 kilogram high-pressure homogeneous.Adopt the instantaneous sterilizing method sterilizing of 115 DEG C of sterilizing 5s, product is tested, is up to the standards and packs, make sea cucumber cordyceps drink.
Embodiment 11
Get sea cucumber extract, Chinese caterpillar fungus extract, STEVIA REBAUDIANA, ethyl maltol, sodium carboxymethylcellulose, citric acid, white granulated sugar, by mass percentage: sea cucumber extract 1.0%, Chinese caterpillar fungus extract 0.032%, STEVIA REBAUDIANA 0.2%, ethyl maltol 0.02%, sodium carboxymethylcellulose 0.1%, citric acid 0.1%, white granulated sugar 2%, all the other are water.Prepare by each component, packing after twice high-pressure homogeneous.First time is high-pressure homogeneously: 170-180 kilogram high-pressure homogeneous; Second time high-pressure homogeneous be 250-280 kilogram high-pressure homogeneous.Adopt the instantaneous sterilizing method sterilizing of 115 DEG C of sterilizing 5s, be up to the standards, pack, make sea cucumber cordyceps militaris oral liquid.
Embodiment 12
Get sea cucumber extract, Chinese caterpillar fungus extract, STEVIA REBAUDIANA, ethyl maltol, sodium carboxymethylcellulose, citric acid, white granulated sugar, by mass percentage: sea cucumber extract 1.5%, Chinese caterpillar fungus extract 0.02%, STEVIA REBAUDIANA 0.3%, ethyl maltol 0.02%, sodium carboxymethylcellulose 0.15%, citric acid 0.15%, white granulated sugar 2%, all the other are water.Prepare by each component, packing after twice high-pressure homogeneous.First time is high-pressure homogeneously: 170-180 kilogram high-pressure homogeneous; Second time high-pressure homogeneous be 250-280 kilogram high-pressure homogeneous.Adopt the instantaneous sterilizing method sterilizing of 115 DEG C of sterilizing 5s, be up to the standards, pack, make sea cucumber cordyceps militaris oral liquid.
Embodiment 13
Get sea cucumber extract, Chinese caterpillar fungus extract, STEVIA REBAUDIANA, ethyl maltol, sodium carboxymethylcellulose, citric acid, white granulated sugar, by mass percentage: sea cucumber extract 1.6%, Chinese caterpillar fungus extract 0.032%, STEVIA REBAUDIANA 0.3%, ethyl maltol 0.03%, sodium carboxymethylcellulose 0.15%, citric acid 0.15%, white granulated sugar 2%, all the other are water.Prepare by each component, packing after twice high-pressure homogeneous.First time is high-pressure homogeneously: 170-180 kilogram high-pressure homogeneous; Second time high-pressure homogeneous be 250-280 kilogram high-pressure homogeneous.Adopt the instantaneous sterilizing method sterilizing of 115 DEG C of sterilizing 5s, be up to the standards, pack, make sea cucumber cordyceps militaris oral liquid.
Adopt the sea cucumber cordyceps militaris liquor that embodiment 5-7 makes, concrete effect is as follows:
Application examples
Case 1
Liu so-and-so, man, 59 years old, there is 38 year wine storage time, take front physique very weak, have the symptom such as numbness in hands and feet, easy catching a cold, through taking sea cucumber cordyceps militaris liquor after 5 months, all symptoms are all alleviated, physique strengthens, and complexion is white touched with red, and verbal facility is pure, and overall mental attitude is taken on an entirely new look.
Case 2
Chen, female, 57 years old, take obscure, the frequent insomnia and dreamful sleep of colour, memory be poor, every day upstairs time can have a dizzy spell, through taking sea cucumber cordyceps militaris liquor after 4 months, all symptoms are all alleviated, and have rosy cheeks glossy, sleep quality significantly promotes, phenomenon of having a dizzy spell upstairs disappears, and the spiritual gas of people is also sufficient.
The user 30 being taken to sea cucumber cordyceps militaris liquor of the present invention carries out tracing observation, whether its immunity of main detection improves, result is as follows: 30 users took sea cucumber cordyceps militaris liquor of the present invention after 4 months, user feels that autoimmunity improves a lot, and sleep quality also significantly improves.
Adopt the sea cucumber cordyceps drink that embodiment 8-10 makes, concrete effect is as follows:
Application examples
Case 1
Lee so-and-so, female, 35 years old, company worker, took poor memory before sea cucumber cordyceps drink, neural sensitivity, insomnia is irritated, take sea cucumber cordyceps drink after 3 months, she feels that condition becomes and becomes better and better, and other people of company ask her, what have been eaten recently, perceptual image is that to have changed a people the same, her confidence of taking that this is more firm.
Case 2
Lee so-and-so, man, 56 years old, taking front in poor condition of health, having hypertension, often headache, dizziness, through taking sea cucumber cordyceps drink after 5 months, headache, dizziness disappear, the systolic pressure of blood pressure has dropped to 125mmHg by original 140mmHg, and diastolic pressure has dropped to 80mmHg by original 100mmHg, and pressure value is tending towards normal value.
The user 20 being taken to sea cucumber cordyceps drink of the present invention carries out tracing observation, whether its corresponding illness of main detection is improved, result is as follows: taking sea cucumber cordyceps drink after 3 months, 20 user's body qualities all make moderate progress, have rosy cheeks glossy, memory significantly improves, and has a headache, irritated symptom of having a sleepless night alleviates.
Adopt the sea cucumber cordyceps militaris oral liquid that embodiment 11-13 makes, concrete effect is as follows:
Application examples
Case 1
King so-and-so, female, 68 years old, suffers from cerebral thrombus before taking, Calf muscle atrophy, moving difficulty, takes sea cucumber cordyceps militaris oral liquid after 6 months, and Wang Laotai often leans on cane and strolls in community, and people seems also to be filled with happy.
Case 2
Liu, man, 49 years old, enterprise boss, suffers from high fat of blood, hypertension, atherosclerotic before taking, through taking sea cucumber cordyceps militaris oral liquid after 3 months, dizziness, extremity numbness, uncomfortable in chest, cardio palmus shape alleviate, and blood pressure does not also steadily fluctuate, and quality of life is significantly improved.
The user 30 being taken to sea cucumber cordyceps militaris oral liquid of the present invention carries out tracing observation, whether its corresponding illness of main detection is improved, result is as follows: taking sea cucumber cordyceps militaris oral liquid after 3 months, 30 user's body qualities significantly improve, and corresponding illness alleviates and even disappears.
Claims (10)
1. contain a composition for sea cucumber and Chinese caterpillar fungus active ingredient, it is characterized in that, the composition of described composition comprises: sea cucumber extract and Chinese caterpillar fungus extract.
2. composition according to claim 1, is characterized in that, described composition contains sea cucumber extract 0.2%-1.6% (w/v), Chinese caterpillar fungus extract in cordycepin content for 0.002%-0.032% (w/v).
3. prepare the method for composition described in claim 1, it is characterized in that comprising the following step:
S1 prepares sea cucumber extract;
S2 prepares Chinese caterpillar fungus extract;
The Product mix that S1 and S2 is prepared by S3 evenly makes composition.
4. preparation method according to claim 3, is characterized in that, the concrete steps of described S1 are:
Sea cucumber gills by S1.1 cleaning, cleans silt;
Clean sea cucumber is placed in hydrolytic decomposition pot by S1.2 pretreatment, adds water;
Step S1.2 product is made after sea cucumber milk high-pressure homogeneous through beater by S1.3 slurrying;
S1.4 enzymolysis adds the protease hydrolyzed of 0.3-0.5% (v/v) in the obtained product of S1.3, and go out thereafter enzyme;
S1.5 separation and Extraction is by enzymolysis liquid centrifugal filtration, and it is for subsequent use to get filtrate;
Filtrate is concentrated by vacuum concentration pot by S1.6 reduced pressure concentration, and the concentrate of acquisition is sea cucumber extract.
5. preparation method according to claim 3, is characterized in that, the concrete steps of described S2 are:
It is dry in microwave vacuum dryer that S2.1 drying and crushing gets Chinese caterpillar fungus, after pulverizer is pulverized, obtains Chinese caterpillar fungus powder;
S2.2 is concentrated is fully dissolved in water by step S2.1 product, filters, gets filtrate and concentrate in vacuum concentration pot, obtain Chinese caterpillar fungus extract after lixiviate.
6. the preparation method according to claim arbitrary in claim 3-5, it is characterized in that, preparation process S2 also comprises: S2.3 determines the content of cordycepin.
7. preparation method according to claim 6, is characterized in that, described step S2.3 adopts ultraviolet spectrophotometry.
8. the composition containing sea cucumber and Chinese caterpillar fungus described in the arbitrary claim of claim 1 or 2 is preparing the application in health products or food.
9. health products or a food, wherein containing composition described in the arbitrary claim of claim 1 or 2 as active ingredient; Described health products or food are oral formulations.
10. health products according to claim 9 or food, described health products or food are oral liquid, medicinal liquor and beverage.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106399010A (en) * | 2016-08-31 | 2017-02-15 | 辽宁双增生物工程有限公司 | Preparation method of sea cucumber and cordyceps sinensis wine |
CN107307332A (en) * | 2017-07-27 | 2017-11-03 | 大连真久生物产业有限公司 | Double ginseng Cordyceps militaris liquid |
CN116019186A (en) * | 2023-01-30 | 2023-04-28 | 江西沐恩堂生物科技有限公司 | Sea cucumber polypeptide drink and preparation method thereof |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1600163A (en) * | 2004-10-18 | 2005-03-30 | 何方文 | Health food of containing sea cucumber and Chinese caterpillar fungus |
CN101194733A (en) * | 2007-12-26 | 2008-06-11 | 大连工业大学 | Holothurian compound nutrition foods and production method thereof |
CN102090680A (en) * | 2009-12-14 | 2011-06-15 | 谭攸恒 | Sea cucumber drink and preparation method thereof |
CN103120302A (en) * | 2011-11-18 | 2013-05-29 | 大连得达科技发展有限公司 | Making method of sea cucumber medicinal liquor |
CN103555552A (en) * | 2013-11-20 | 2014-02-05 | 中国科学院北京基因组研究所 | Method for preparing healthcare cordyceps militaris vinegar by virtue of secondary fermentation |
CN104137981A (en) * | 2013-05-06 | 2014-11-12 | 宁波海逸生物科技有限公司 | Cordyceps militaris and sea cucumber tablet formula |
CN104872485A (en) * | 2015-04-30 | 2015-09-02 | 青岛金谷原生命科技有限公司 | Preparation method of tablets containing sea cucumber, cordyceps sinensis and propolis |
-
2016
- 2016-01-05 CN CN201610005156.8A patent/CN105558896A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1600163A (en) * | 2004-10-18 | 2005-03-30 | 何方文 | Health food of containing sea cucumber and Chinese caterpillar fungus |
CN101194733A (en) * | 2007-12-26 | 2008-06-11 | 大连工业大学 | Holothurian compound nutrition foods and production method thereof |
CN102090680A (en) * | 2009-12-14 | 2011-06-15 | 谭攸恒 | Sea cucumber drink and preparation method thereof |
CN103120302A (en) * | 2011-11-18 | 2013-05-29 | 大连得达科技发展有限公司 | Making method of sea cucumber medicinal liquor |
CN104137981A (en) * | 2013-05-06 | 2014-11-12 | 宁波海逸生物科技有限公司 | Cordyceps militaris and sea cucumber tablet formula |
CN103555552A (en) * | 2013-11-20 | 2014-02-05 | 中国科学院北京基因组研究所 | Method for preparing healthcare cordyceps militaris vinegar by virtue of secondary fermentation |
CN104872485A (en) * | 2015-04-30 | 2015-09-02 | 青岛金谷原生命科技有限公司 | Preparation method of tablets containing sea cucumber, cordyceps sinensis and propolis |
Non-Patent Citations (5)
Title |
---|
刘建华 等: "《食用菌保鲜与加工实用新技术》", 31 January 2008, 陕西科学技术出版社 * |
吕丹等: "UV法和HPLC法测定北虫草中虫草素含量的方法比较", 《广东化工》 * |
孙军德等: "蛹虫草中虫草素测定方法的比较", 《沈阳农业大学学报》 * |
梁生旺: "《中药药剂分析》", 30 April 2013, 中国中医药出版社 * |
王陶等: "虫草素的微波-超声波协同提取", 《食品科学》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106399010A (en) * | 2016-08-31 | 2017-02-15 | 辽宁双增生物工程有限公司 | Preparation method of sea cucumber and cordyceps sinensis wine |
CN107307332A (en) * | 2017-07-27 | 2017-11-03 | 大连真久生物产业有限公司 | Double ginseng Cordyceps militaris liquid |
CN116019186A (en) * | 2023-01-30 | 2023-04-28 | 江西沐恩堂生物科技有限公司 | Sea cucumber polypeptide drink and preparation method thereof |
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