CN105558528A - Anti-fatigue Maca extract and functional drink including same - Google Patents
Anti-fatigue Maca extract and functional drink including same Download PDFInfo
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Abstract
The invention relates to an anti-fatigue Maca extract and a functional drink including the same. The anti-fatigue Maca extract is prepared from the following raw materials by mass: 0.8 to 1.2 of Maca powder, 0.1 to 0.3 of rhodiola root powder, 0.2 to 0.5 of yacon powder, 0.2 to 0.5 of crab apple powder and 0.08 to 0.12 of mint leaf powder. According to the invention, the Maca, rhodiola root and yacon are blended, so comprehensive anti-fatigue effect is exerted; the crab apple and mint leaf enable the beverage to has sour-sweet, cool and refreshing taste; through microwave treatment of Maca powder, the irritant and pungent taste of Maca beverages can be eliminated; and anti-fatigue accessory factors are added for coordinate regulation of amino acid metabolism, carbohydrate decomposition and lipid metabolism of the body, so body fatigue caused by acute exercises of the human body is regulated, the function of reservation of hepatic glycogen is improved, etc.
Description
Technical field
The invention belongs to field of health drinks, in particular, relate to a kind of antifatigue Maca extract and comprise the drinks of this extract.
Background technology
Fatigue refers to that organic ability to work weakens gradually, and it is not equal to the exhaustion of muscle power, but will prevent the warning of exhaustion, is the reaction of organic a kind of self-protection.Tired essence is the physiological phenomenon of organic a kind of defensive self-protection.
Produce tired reason:
(1) energy substance consumption: the consumption of the phosphocreatine of storage in body.
(2) accumulation of metabolic waste: lactic acid---hinder the excited transmission at myoneural junction place; Phosphofructokinase is suppressed to slow down glycolytic cycle; Substitute the calcium in troponin, reduce contraction of muscle ability; Act on brain, cause the side effects such as pain is felt sick, disorientation; Suppress the activity of sensitive lipase in adipose tissue, restraint of liberty aliphatic acid enters blood.
(3) environment regulates: in body, pH value declines, and the factors such as water and salt metabolic disturbance, plasma osmolarity change cause.
(4) protectiveness suppresses: produced by cerebral cortex, brain nervous cell is overexcited, and energy ezpenditure is too much, when being depleted to a certain degree, in order to the supply of neuroprotective cellular energy, starts to suppress motion.
(5) catastrophe theory: energy delivery rate is inadequate, or muscle strength declines, also may be E-C coupling function generation obstacle and cause occurring fatigue, change between these factors is not linear, in the evolution of muscular fatigue, there is the process of attenuating mutation gradually of different approaches, just as a chain break phenomenon.
(6) free radical theory: during motion, free radical generates too much, by polybasic unsaturated fatty acid on attack biomembrane, produces MDA, causes biomembranous dysfunction, cause intraor extracellular ion transport to get muddled, cause exercise induced fatigue.
Exercise induced fatigue comprises physiologic fatigability (human body energy consumption, shows as DOMS tired, unable etc., and locomitivity declines) and psychological fatigue (neurolysis, main manifestations is study and inefficiency, depression and anxiety).The central mechanism that exercise induced fatigue produces: a large amount of consumption mainly containing sugar in brain cell, ammonia produces increase and Scavenging activity declines; In brain, inhibitory neurotransmitter GABA (GABA), serotonin (5-HT) concentration raise, degradation under dopamine (DA), acetylcholine (Ach) concentration, thus cause central nerve fatigue.
With the quickening pace of modern life, work and social life make people's health day by day dog-tired, and people also face very large pressure in spirit, are easy to produce sense of fatigue.This state has a strong impact on the quality of life of people, and therefore people produce urgent demand by the prevention of fatigue with treatment.In recent years, find anti-fatigue effect remarkable, mouthfeel good drinks become study hotspot.
Agate coffee (Maca, Lepidiummeyenii) belongs to the herbaceous plant of Cruciferae separate row Vegetable spp, originates in the mountain area, Andean of Peru height above sea level 3500 ~ 4500m.As conventional food or medicinal plant, the agate coffee history of edible existing several thousand in South America, main edible part is ripe loose root, the multiple health problem caused because of subalimentation can be solved, have release the pressure, antifatigue, raising fertility, anticancer, alleviate many-sided effect such as climacteric metancholia, improving water flood.In recent years, agate coffee shows significant effect in antifatigue, enhance tolerance, external neuroprotective and hypoxic-ischemic brain damage.The rich in nutrition content of agate coffee; amino acid wherein; mineral matter zinc, the composition energy obviously antifatigues such as taurine, such as taurine can strengthen myocardial contractive power; increase blood to export; prevent myocardial damage simultaneously and can liver be protected, can also locomitivity be maintained, strengthen supply rapidly; the locomitivity of human body and anti-fatigue ability are increased further, to reach the effect of remarkable antifatigue.
No. CN102578653A patent discloses a kind of Maca functional drink and preparation method thereof, this beverage ingredient is more single, allocated by fine maca China liquid and form, only solve physical fatigue problem by makeup energy, and find out from preparation method, adopt simple edible alcohol extraction that Maca beverage can be caused to have acid, mouthfeel is bad.
No. CN102362685A patent discloses a kind of Maca composite beverage and preparation method thereof, this patent prescription is mainly agate coffee, okra, male silk moth etc., prescription thinking, mainly strengthen the thinking of male sextual function, and (male silk moth can bring certain fishy smell), anti-sports fatigue effect and suitable population also have certain not enough in mouthfeel.
Summary of the invention
Technical problem to be solved by this invention is: propose a kind of antifatigue Maca extract and comprise the drinks of this extract, solve existing antifatigue Maca extract or Maca functional drink composition is single, can not the problem such as the mouthfeel that causes of the acid of relieving fatigue problem, agate coffee is not comprehensively good.
The technical scheme that the present invention solves the problem is:
There is provided a kind of antifatigue Maca extract, the mass ratio preparing the feed components of described Maca extract is:
Pueraria root powder 0.8 ~ 1.2, rhodiola root powder 0.1 ~ 0.3, Smallantus sonchifolium powder 0.2 ~ 0.5, calophyllum inophyllum powder 0.2 ~ 0.5, dried peppermint leaf powder 0.08 ~ 0.12.
In antifatigue Maca extract provided by the invention, described pueraria root powder obtains in the following way: get fresh of agate coffee, cleans, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves; Described rhodiola root powder obtains in the following way: get fresh root of Rhodiola sachalinensis, cleans, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves; Described Smallantus sonchifolium powder obtains in the following way: get Smallantus sonchifolium, peeling after cleaning, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves; Described calophyllum inophyllum powder obtains in the following way: get fresh calophyllum inophyllum, peeling after cleaning, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves; Described dried peppermint leaf powder obtains in the following way: get fresh mint leaf, cleaning, drying, pulverizes, and crosses 80 ~ 100 mesh sieves.
In antifatigue Maca extract provided by the invention, described antifatigue Maca extract uses as under type obtains:
S1, described pueraria root powder and water are according to solid-liquid ratio 1: 5 ~ 1: 7, at temperature 55 ~ 65 DEG C, and first time refluxing extraction 2 ~ 4h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 3 ~ 1: 5, at temperature 55 ~ 65 DEG C, and second time refluxing extraction 1 ~ 3h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 1 ~ 1: 3, at temperature 55 ~ 65 DEG C, and third time refluxing extraction 1 ~ 3h, filters, obtain supernatant; Merge three supernatants and obtain extract;
S2, in described extract, add the complex enzyme of neutral proteinase and flavor protease according to preset ratio, regulate pH to 6.0 ~ 7.5, temperature is water enzyme digestion 3 ~ 6h at 40 ~ 55 DEG C, obtains enzymolysis liquid;
S3, in described enzymolysis liquid, add described rhodiola root powder, Smallantus sonchifolium powder, calophyllum inophyllum powder, dried peppermint leaf powder, refluxing extraction 1 ~ 3h at temperature 55 ~ 65 DEG C, then raised temperature to 95 DEG C backflow 15min, filters after being cooled to room temperature, filtrate is condensed into medicinal extract, namely obtains Maca extract after drying and crushing.
In antifatigue Maca extract provided by the invention, also comprise before described step S1:
S0, described pueraria root powder are placed in microwave dryer, under 2450MHz microwave, process 3min ~ 5min.
In antifatigue Maca extract provided by the invention, in described step S2, described preset ratio is prepare the pueraria root powder of described extract and the ratio of complex enzyme, every gram of pueraria root powder adds the complex enzyme of 8000 ~ 12000U, the enzyme work of neutral proteinase is 200,000 U/g, and the enzyme work of flavor protease is 200,000 U/g; The mass ratio of described neutral proteinase and flavor protease is 1:1 ~ 3:1.
The present invention also provides a kind of antifatigue Maca functional drink, comprises described antifatigue Maca extract, and the mass ratio that described Maca extract accounts for described Maca functional drink is 1% ~ 7%.
In antifatigue Maca functional drink of the present invention, described Maca functional drink also comprises the component of following mass ratio:
VA0.1 ‰ ~ 0.5 ‰, VC0.25 ‰ ~ 0.5 ‰, VB10.001 ‰ ~ 0.003 ‰, VB20.001 ‰ ~ 0.003 ‰, VB50.002 ‰ ~ 0.004 ‰, nicotinic acid 0.003 ‰ ~ 0.018 ‰, branched-chain amino acid 1% ~ 3%.
In antifatigue Maca functional drink of the present invention, described Maca functional drink also comprises the component of following mass ratio:
HFCS 2% ~ 6%, citric acid 0.04% ~ 0.07%, xanthans 0.01% ~ 0.07%.
In antifatigue Maca functional drink of the present invention, described Maca functional drink is sterilizing 4 ~ 15S at 135 ~ 150 DEG C, and sterile filling after cooling, carries out spray sterilizing after filling, get product.
The beneficial effect that enforcement the present invention obtains is:
1, agate coffee and rhodiola root, Smallantus sonchifolium are allocated the effect strengthening antifatigue, and add calophyllum inophyllum and dried peppermint leaf wherein and make beverage mouthfeel naturally sour-sweet and refrigerant tasty and refreshing.The polypeptide be rich in beverage, taurine, branched-chain amino acid, rhodioloside, polysaccharide, compound sugar, organic acid, mineral matter etc. can provide rapidly energy, and delay fatigue produces, and accelerates elimination of fatigue.And add the antifatigue confactors such as VA, VC, VB1, VB2, VB5, nicotinic acid in this beverage, synergic adjustment body amino acid metabolism, carbohydrate breakdown and Regulating Lipid Metabolism, reconcile human body acute exercise and cause organism fatigue, strengthen the deposit function etc. of hepatic glycogen.
2, before refluxing extraction by pueraria root powder through microwave treatment, the glucosinolate in agate coffee can be removed, improve agate coffee Odor stimulation, the problem that mouthfeel is pungent.
3, the agate coffee extract of gained is through compound protein enzymolysis, object produces the polypeptide and amino acid that more easily absorb after a large amount of protein in agate coffee extract are carried out enzymolysis, compound protease determines through screening the combination adopting restriction endonuclease and excision enzyme, not only improve enzymolysis efficiency but also decrease the generation of bitter taste, the beverage made by the Maca extract of gained after ferment treatment, mouthfeel, smell are better, polypeptide and amino acid are also more stable than a large amount of protein, not easily produce floccule and precipitation in high-temperature sterilization and storage.
4, calophyllum inophyllum sweet mouthfeel in beverage of the present invention, the smell of fruits is very sweet, and dried peppermint leaf taste is refrigerant, allocates using HFCS as sweetener, makes beverage sweet and sour taste, refrigerant brisk and neat.This beverage is that pure natural raw material extracts, without any side effects, and by mouse experiment, has remarkable anti-fatigue effect.
Detailed description of the invention
Be clearly and completely described to the technical scheme in the embodiment of the present invention below, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Existing antifatigue Maca extract or Maca functional drink are mainly using agate coffee extract as raw material, and composition is single, can not relieving fatigue problem comprehensively, and the acid of agate coffee can cause the mouthfeel of beverage not good etc. problem.Main innovate point of the present invention is: agate coffee and rhodiola root, Smallantus sonchifolium are allocated using, can play comprehensive antifatigue effect, and calophyllum inophyllum and dried peppermint leaf make beverage mouthfeel sour-sweet and refrigerant tasty and refreshing; Antifatigue confactor add synergic adjustment body amino acid metabolism, carbohydrate breakdown and Regulating Lipid Metabolism, reconcile human body acute exercise and cause organism fatigue, strengthen the deposit function etc. of hepatic glycogen; Microwave treatment pueraria root powder, to remove glucosinolate, eliminates the stimulation of agate coffee, pungent mouthfeel.
In antifatigue Maca extract provided by the invention, the mass ratio of feed components is:
Pueraria root powder 0.8 ~ 1.2, rhodiola root powder 0.1 ~ 0.3, Smallantus sonchifolium powder 0.2 ~ 0.5, calophyllum inophyllum powder 0.2 ~ 0.5, dried peppermint leaf powder 0.08 ~ 0.12.
Be rich in protein in rhodiola root, glucoside, reducing substances etc., there is nourishing vigour, anti anoxia, antifatigue, improve the effects such as mental and physical function.In motion process, human body is often in anaerobic condition, and rhodiola root can significantly improve anti-anoxia ability, promotes body aerobic metabolism, promotes that body is to the adaptation of anaerobic environment, and the generation of delay fatigue.Rhodiola root can reduce the serotonin level in brain, and serotonin is the material causing central nerve fatigue.
Be rich in needed by human body 20 several amino acids and multivitamin, mineral matter in Smallantus sonchifolium, and the content of compound sugar is very high, compound sugar can extend energization time, strengthens body endurance, has anti-fatigue effect; Smallantus sonchifolium can be anti-oxidant, eliminate free radical, and Smallantus sonchifolium is cool in nature, lower fire, can in and agate coffee may cause the effect of getting angry.
Calophyllum inophyllum contains a large amount of nutriments, as carbohydrate, multivitamin etc., can supplement the nutrition of needed by human body, improves body function, builds up resistance.Taste is sour-sweet, containing strong fresh Malus spectabilis fragrance, and refrigerant purging intense heat, spleen benefiting and stimulating the appetite, has good food therapy health effect.
The preparation process of this antifatigue Maca extract is as follows:
A. described pueraria root powder obtains in the following way: get fresh of agate coffee, cleans, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves for subsequent use.
B. described rhodiola root powder obtains in the following way: get fresh root of Rhodiola sachalinensis, cleans, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves for subsequent use.
C. described Smallantus sonchifolium powder obtains in the following way: get Smallantus sonchifolium, peeling after cleaning, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves for subsequent use.
D. described calophyllum inophyllum powder obtains in the following way: get fresh calophyllum inophyllum, peeling after cleaning, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves for subsequent use.
E. described dried peppermint leaf powder obtains in the following way: get fresh mint leaf, cleaning, drying, after pulverizing, crosses 80 ~ 100 mesh sieves for subsequent use.
F. pueraria root powder is placed in microwave dryer, under 2450MHz microwave, process 3min ~ 5min.Microwave treatment can remove the glucosinolate in agate coffee, although glucosinolate also has certain physiologically active, the effect for antifatigue is not obvious, and a lot of crowd decomposes to glucosinolate in Maca beverage the pungent flavour and distinctive odor that produce and uncomfortable.
G. pueraria root powder and water are according to solid-liquid ratio 1: 5 ~ 1: 7, at temperature 55 ~ 65 DEG C, and first time refluxing extraction 2 ~ 4h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 3 ~ 1: 5, at temperature 55 ~ 65 DEG C, and second time refluxing extraction 1 ~ 3h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 1 ~ 1: 3, at temperature 55 ~ 65 DEG C, and third time refluxing extraction 1 ~ 3h, filters, obtain supernatant; Merge three supernatants and obtain extract.
H. in extract, add the complex enzyme of neutral proteinase and flavor protease according to preset ratio, regulate pH to 6.0 ~ 7.5, temperature is water enzyme digestion 3 ~ 6h at 40 ~ 55 DEG C, obtains enzymolysis liquid; Preferably, preset ratio is prepare the pueraria root powder of extract and the ratio of complex enzyme, and every gram of pueraria root powder adds the complex enzyme of 8000 ~ 12000U, and the enzyme work of neutral proteinase is 200,000 U/g, and the enzyme work of flavor protease is 200,000 U/g; The mass ratio of described neutral proteinase and flavor protease is 1:1 ~ 3:1.
I. according to described ratio above, add rhodiola root powder, Smallantus sonchifolium powder, calophyllum inophyllum powder, dried peppermint leaf powder, refluxing extraction 1 ~ 3h at 55 ~ 65 DEG C of temperature in enzymolysis liquid, then raised temperature to 95 DEG C backflow 15min, makes neutral proteinase and flavor protease deactivation; Filter after being cooled to room temperature, filtrate is condensed into medicinal extract, namely obtains Maca extract after drying and crushing.
Present invention also offers a kind of antifatigue Maca functional drink, use described Maca extract obtained above, the mass ratio that this Maca extract accounts for Maca functional drink is 1% ~ 7%, and preparation process is as follows:
A, preparation: by the Maca extract of preparation, add the pure water of metering ratio, then adds the antifatigue confactor by component proportion metering and/or additive, stir, mix, obtain Maca functional drink.
B, sterilizing, filling: Maca functional drink is sterilizing 4 ~ 15S at 135 ~ 150 DEG C, filling after cooling, carries out spray sterilizing, the antifatigue that gets product Maca functional drink after filling.
Wherein, antifatigue confactor is the component of following mass ratio:
VA (vitamin A) 0.1 ‰ ~ 0.5 ‰, VC (vitamin C) 0.25 ‰ ~ 0.5 ‰, VB1 (vitamin B1) 0.001 ‰ ~ 0.003 ‰, VB2 (vitamin B2) 0.001 ‰ ~ 0.003 ‰, VB5 (vitamin B5) 0.002 ‰ ~ 0.004 ‰, nicotinic acid 0.003 ‰ ~ 0.018 ‰, branched-chain amino acid 1% ~ 3%.
Additive is the component of following mass ratio: HFCS 2% ~ 6%, citric acid 0.04% ~ 0.07%, xanthans 0.01% ~ 0.07%.
The preparation of embodiment 1 antifatigue Maca extract
I, agate coffee, rhodiola root, Smallantus sonchifolium, calophyllum inophyllum, dried peppermint leaf, all dry, pulverize, to cross 80 mesh sieves for subsequent use;
Pueraria root powder is placed in microwave dryer, under 2450MHz microwave, process 3min;
Each component of following mass ratio is used to prepare antifatigue Maca extract:
Pueraria root powder 0.8, rhodiola root powder 0.3, Smallantus sonchifolium powder 0.5, calophyllum inophyllum powder 0.5, dried peppermint leaf powder 0.12;
Pueraria root powder and water are according to solid-liquid ratio 1: 7, at temperature 65 DEG C, and first time refluxing extraction 4h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 3, at temperature 55 DEG C, and second time refluxing extraction 1h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 1, at temperature 55 DEG C, and third time refluxing extraction 1h, filters, obtain supernatant; Merge three supernatants and obtain extract;
In extract, add 8000U complex enzyme according to every gram of pueraria root powder raw material, regulate pH to 6.0, temperature is water enzyme digestion 3h at 55 DEG C, obtains enzymolysis liquid.The mass ratio of complex enzyme neutral and alkali protease and papain is 1:1; The enzyme work of neutral proteinase is 200,000 U/g, and the enzyme work of flavor protease is 200,000 U/g, lower same;
Rhodiola root powder, Smallantus sonchifolium powder, calophyllum inophyllum powder, dried peppermint leaf powder is added in enzymolysis liquid, refluxing extraction 3h at 55 DEG C of temperature, then raised temperature to 95 DEG C backflow 15min, filters after being cooled to room temperature, filtrate is condensed into medicinal extract, namely obtains Maca extract after drying and crushing.
II, agate coffee, rhodiola root, Smallantus sonchifolium, calophyllum inophyllum, dried peppermint leaf, all dry, pulverize, to cross 90 mesh sieves for subsequent use;
Pueraria root powder is placed in microwave dryer, under 2450MHz microwave, process 4min;
Each component of following mass ratio is used to prepare antifatigue Maca extract:
Pueraria root powder 1.2, rhodiola root powder 0.1, Smallantus sonchifolium powder 0.2, calophyllum inophyllum powder 0.2, dried peppermint leaf powder 0.08.
Pueraria root powder and water are according to solid-liquid ratio 1: 5, at temperature 55 DEG C, and first time refluxing extraction 2h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 5, at temperature 65 DEG C, and second time refluxing extraction 3h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 3, at temperature 65 DEG C, and third time refluxing extraction 3h, filters, obtain supernatant; Merge three supernatants and obtain extract;
In extract, add 12000U complex enzyme according to every gram of pueraria root powder raw material, regulate pH to 7.5, temperature is water enzyme digestion 6h at 45 DEG C, obtains enzymolysis liquid.The mass ratio of complex enzyme neutral and alkali protease and papain is 3:1;
Rhodiola root powder, Smallantus sonchifolium powder, calophyllum inophyllum powder, dried peppermint leaf powder is added in enzymolysis liquid, refluxing extraction 1h at 65 DEG C of temperature, then raised temperature to 95 DEG C backflow 15min, filters after being cooled to room temperature, filtrate is condensed into medicinal extract, namely obtains Maca extract after drying and crushing.
III, agate coffee, rhodiola root, Smallantus sonchifolium, calophyllum inophyllum, dried peppermint leaf, all dry, pulverize, to cross 100 mesh sieves for subsequent use;
Pueraria root powder is placed in microwave dryer, under 2450MHz microwave, process 5min;
Each component of following mass ratio is used to prepare antifatigue Maca extract:
Pueraria root powder 1.0, rhodiola root powder 0.2, Smallantus sonchifolium powder 0.3, calophyllum inophyllum powder 0.3, dried peppermint leaf powder 0.1.
Pueraria root powder and water are according to solid-liquid ratio 1: 6, under temperature 60 C, and first time refluxing extraction 3h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 4, under temperature 60 C, and second time refluxing extraction 2h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 2, under temperature 60 C, and third time refluxing extraction 2h, filters, obtain supernatant; Merge three supernatants and obtain extract;
In extract, add 10000U complex enzyme according to every gram of pueraria root powder raw material, regulate pH to 7.0, temperature is water enzyme digestion 4h at 50 DEG C, obtains enzymolysis liquid.The mass ratio of complex enzyme neutral and alkali protease and papain is 2:1;
Rhodiola root powder, Smallantus sonchifolium powder, calophyllum inophyllum powder, dried peppermint leaf powder is added in enzymolysis liquid, refluxing extraction 2h at 60 DEG C of temperature, then raised temperature to 95 DEG C backflow 15min, filters after being cooled to room temperature, filtrate is condensed into medicinal extract, namely obtains Maca extract after drying and crushing.
Sum up, the extraction efficiency of above-mentioned 3 kinds of mode gained Maca extract and species activity thing, without obvious difference, consider energy consumption and extraction time, and in elite fixed III, Maca extract is as the preparation of follow-up antifatigue Maca functional drink.
The preparation of embodiment 2 antifatigue Maca functional drink
I, pure water is added to Maca extract, then VA0.1 ‰, VC0.25 ‰, VB10.001 ‰, VB20.001 ‰, VB50.002 ‰, nicotinic acid 0.003 ‰, branched-chain amino acid 1%, HFCS 2%, citric acid 0.04%, xanthans 0.01% is added, stir, mix, obtain Maca functional drink.Wherein Maca extract accounts for the mass ratio of Maca functional drink is 1%.
Maca functional drink carries out sterilizing 15S at 135 DEG C, and aseptic sterile filling after cooling, carries out spray sterilizing after filling, the antifatigue that gets product Maca functional drink.
II, pure water is added to Maca extract, then VA0.5 ‰, VC0.5 ‰, VB10.003 ‰, VB20.003 ‰, VB50.004 ‰, nicotinic acid 0.018 ‰, branched-chain amino acid 3%, HFCS 6%, citric acid 0.07%, xanthans 0.07% is added, stir, mix, obtain Maca functional drink.Wherein Maca extract accounts for the mass ratio of Maca functional drink is 7%.
Maca functional drink carries out sterilizing 4S at 150 DEG C, and sterile filling after cooling, carries out spray sterilizing after filling, the antifatigue that gets product Maca functional drink.
III, pure water is added to Maca extract, then VA0.3 ‰, VC0.4 ‰, VB10.002 ‰, VB20.002 ‰, VB50.003 ‰, nicotinic acid 0.01 ‰, branched-chain amino acid 2%, HFCS 4%, citric acid 0.05%, xanthans 0.04% is added, stir, mix, obtain Maca functional drink.Wherein Maca extract accounts for the mass ratio of Maca functional drink is 3%.
Maca functional drink carries out sterilizing 8S at 140 DEG C, filling after cooling, carries out spray sterilizing, the antifatigue that gets product Maca functional drink after filling.
Sum up, the active material concentration of above-mentioned 3 kinds of mode gained antifatigue Maca functional drink is different, elite determine active material concentration moderate III in gained antifatigue Maca functional drink carry out compliance test result.
Embodiment 3 compliance test result
Mouse experiment has been carried out with Maca functional drink selected in distilled water and embodiment 2, according to the requirement contrived experiment of " the anti-fatigue effect method of inspection " in country's " health food inspection and assessment technical specification ", Evaluation on anti-fatigue effect is carried out to this Maca functional drink.
Animal used as test used is male mouse of kunming, SPF level, and body weight 20g-24g, is provided by Disease Prevention Control Center, Hubei Prov, and animal used as test production licence is: SCXK (Hubei Province) 2008-0005.Animal feed is provided by Disease Prevention Control Center, Hubei Prov, operative norm GB14924.3-2010.Sense environmental conditions, experimental animal room temperature range 22-26 DEG C, RH range 40%-70%.In Animal House environment, 5 days are adapted to before mouse test.
From the mouse bought, choose 20 be divided into 2 groups at random: control group and beverage group.Beverage group gavage every day beverage 2mL/100g, control group gavage every day distilled water 2mL/100g, continuous gavage is tested after 5 days as follows:
Experiment one: mouse heavy burden 5% swimming, record mouse swimming time.
Experiment two: mouse is without swimming with a load attached to the body after 90 minutes, and cervical dislocation is put to death, gets serum and brain tissue does biochemical indicator detection.
Antifatigue Maca functional drink of the present invention is on the impact of mice burden swimming time, and namely the experimental result of experiment one is as shown in table 1.
The table 1 mice burden swimming time
| Group | Mouse number (only) | The average swim time (dividing) | P value |
| Control group | 10 | 16.51±2.88 | ‐‐‐ |
| Beverage group | 10 | 30.59±6.02 | <0.01 |
Result from table 1 can find out that the power of the beverage group time that exhausts obviously is longer than control group, illustrates that this beverage has anti-fatigue effect.
Antifatigue Maca functional drink of the present invention, on the impact of mouse biochemical indicator, namely tests the experimental result of two as shown in table 2 ~ 9.
Table 2 is on the impact of mice serum LH content
| Group | Mouse number (only) | Blood lactase acid (mmol/l) | P value |
| Control group | 10 | 8.36±1.29 | ‐‐‐ |
| Beverage group | 10 | 6.99±1.02 | <0.01 |
Table 3 is on the impact of mice serum BUN content
| Group | Mouse number (only) | Serum BUN (mmol/l) | P value |
| Control group | 10 | 7.62±1.02 | ‐‐‐ |
| Beverage group | 10 | 5.61±0.32 | <0.01 |
Table 4 is on the impact of Mouse Blood ammonia content
| Group | Mouse number (only) | Blood ammonia (μm ol/l) | P value |
| Control group | 10 | 180.57±32.96 | ‐‐‐ |
| Beverage group | 10 | 141.56±25.42 | <0.01 |
Can see from table 2 ~ 4 and know, beverage group small mouse blood lactase acid, blood urea nitrogen, Blood Ammonia Concentration comparatively all significantly reduce in control group.
Urea nitrogen is the major end products of protein metabolism, and the blood urea nitrogen of beverage group mouse reduces, and the decomposition effectively reducing Mice Body internal protein is described.Lactic acid is the catabolic product of energy substance in motion process, during muscular movement, when lactic acid accumulation to a certain extent, just there will be muscular strength decline, cause locomitivity to decline.Therefore, accelerate the removing of blood lactase acid, the generation of sports fatigue can be slowed down.Beverage group Mouse Blood lactic acid content significantly reduces, and illustrates that product of the present invention serves the effect of relieving sports fatigue.During motion, muscle produces blood ammonia and is discharged into blood, and blood ammonia can pass through blood-brain barrier. and there is toxic action.Brain ammonia increases multiple enzymatic activity can be caused to decline, and ATP again synthesis rate declines.Beverage group Mouse Blood ammonia content reduces the decline can organizing ATP synthesis rate, reaches the effect of antifatigue.
Table 5 is on the impact of Mice brain tissues MDA content
| Group | Mouse number (only) | Brain tissue MDA (nmol/mgprot) | P value |
| Control group | 10 | 4.03±0.89 | ‐‐‐ |
| Beverage group | 10 | 3.43±0.64 | <0.05 |
Table 6 is on the impact of Mice brain tissues SOD content
| Group | Mouse number (only) | Brain tissue SOD (U/mgprot) | P value |
| Control group | 10 | 265.01±17.89 | ‐‐‐ |
| Beverage group | 10 | 197.51±16.01 | <0.01 |
Table 7 is on the impact of Mice brain tissues GSH-Px content
| Group | Mouse number (only) | Brain tissue GSH ?Px | P value |
| Control group | 10 | 33.70±6.51 | ‐‐‐ |
| Beverage group | 10 | 53.32±15.20 | <0.01 |
Can see from table 5 ~ 7 and know, beverage group small mouse MDA, superoxide dismutase content reduce, and brain tissue anti-positive enzymatic activity increases.
Table 8 is on the impact of Mouse Liver glycogen content
| Group | Mouse number (only) | Hepatic glycogen (mg/g) | P value |
| Control group | 10 | 9.73±1.27 | ‐‐‐ |
| Beverage group | 10 | 12.97±1.89 | <0.01 |
Table 9 is on the impact of Mouse Muscle glycogen content
| Group | Mouse number (only) | Muscle glycogen (mg/g) | P value |
| Control group | 10 | 0.55±0.09 | ‐‐‐ |
| Beverage group | 10 | 0.84±0.12 | <0.01 |
Known from table 8 ~ 9, beverage group small mouse hepatic glycogen, muscle glycogen content comparatively all increase in control group.Glycogen is energy reserves material important in body, and its stockage and exercise tolerance are proportionate, muscle glycogen be can employ at any time in skeletal muscle stock the energy, improve muscle glycogen levels and can to delay in prolonged exercise tired generation.Hepatic glycogen reserves play an important role to blood sugar concentration during maintenance prolonged exercise.The content increase of beverage group small mouse hepatic glycogen and muscle glycogen can improve endurance, the generation of delay fatigue.
Conclusion: the Maca functional drink in the present invention has the effect of removing the antifatigues such as kinetic muscular fatigue, enhance tolerance and anti anoxia, simultaneously, significantly can reduce kinetic blood lactase acid to raise, reduce blood urea nitrogen, reduce blood ammonia, increase hepatic glycogen and muscle glycogen, increase brain tissue anti-positive enzymatic activity, reduce the content of malondialdehyde, antifatigue Be very effective.
Claims (9)
1. an antifatigue Maca extract, is characterized in that, the mass ratio preparing the feed components of described Maca extract is:
Pueraria root powder 0.8 ~ 1.2, rhodiola root powder 0.1 ~ 0.3, Smallantus sonchifolium powder 0.2 ~ 0.5, calophyllum inophyllum powder 0.2 ~ 0.5, dried peppermint leaf powder 0.08 ~ 0.12.
2. Maca extract according to claim 1, is characterized in that, described pueraria root powder obtains in the following way: get fresh of agate coffee, cleans, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves; Described rhodiola root powder obtains in the following way: get fresh root of Rhodiola sachalinensis, cleans, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves; Described Smallantus sonchifolium powder obtains in the following way: get Smallantus sonchifolium, peeling after cleaning, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves; Described calophyllum inophyllum powder obtains in the following way: get fresh calophyllum inophyllum, peeling after cleaning, and section, dry, pulverize, and crosses 80 ~ 100 mesh sieves; Described dried peppermint leaf powder obtains in the following way: get fresh mint leaf, cleaning, drying, pulverizes, and crosses 80 ~ 100 mesh sieves.
3. Maca extract according to claim 1, is characterized in that, described antifatigue Maca extract uses as under type obtains:
S1, described pueraria root powder and water are according to solid-liquid ratio 1: 5 ~ 1: 7, at temperature 55 ~ 65 DEG C, and first time refluxing extraction 2 ~ 4h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 3 ~ 1: 5, at temperature 55 ~ 65 DEG C, and second time refluxing extraction 1 ~ 3h, filters, obtain supernatant; Filter residue and water are according to solid-liquid ratio 1: 1 ~ 1: 3, at temperature 55 ~ 65 DEG C, and third time refluxing extraction 1 ~ 3h, filters, obtain supernatant; Merge three supernatants and obtain extract;
S2, in described extract, add the complex enzyme of neutral proteinase and flavor protease according to preset ratio, regulate pH to 6.0 ~ 7.5, temperature is water enzyme digestion 3 ~ 6h at 40 ~ 55 DEG C, obtains enzymolysis liquid;
S3, in described enzymolysis liquid, add described rhodiola root powder, Smallantus sonchifolium powder, calophyllum inophyllum powder, dried peppermint leaf powder, refluxing extraction 1 ~ 3h at temperature 55 ~ 65 DEG C, then raised temperature to 95 DEG C backflow 15min, filters after being cooled to room temperature, filtrate is condensed into medicinal extract, namely obtains Maca extract after drying and crushing.
4. Maca extract according to claim 2, is characterized in that, also comprises before described step S1:
S0, described pueraria root powder are placed in microwave dryer, under 2450MHz microwave, process 3min ~ 5min.
5. Maca extract according to claim 2, it is characterized in that, in described step S2, described preset ratio is prepare the pueraria root powder of described extract and the ratio of complex enzyme, every gram of pueraria root powder adds the complex enzyme of 8000 ~ 12000U, the enzyme work of neutral proteinase is 200,000 U/g, and the enzyme work of flavor protease is 200,000 U/g; The mass ratio of described neutral proteinase and flavor protease is 1:1 ~ 3:1.
6. an antifatigue Maca functional drink, is characterized in that, comprises the antifatigue Maca extract in Claims 1 to 5 described in any one, and the mass ratio that described Maca extract accounts for described Maca functional drink is 1% ~ 7%.
7. Maca functional drink according to claim 6, is characterized in that, described Maca functional drink also comprises the component of following mass ratio:
VA0.1 ‰ ~ 0.5 ‰, VC0.25 ‰ ~ 0.5 ‰, VB10.001 ‰ ~ 0.003 ‰, VB20.001 ‰ ~ 0.003 ‰, VB50.002 ‰ ~ 0.004 ‰, nicotinic acid 0.003 ‰ ~ 0.018 ‰, branched-chain amino acid 1% ~ 3%.
8. Maca functional drink according to claim 6, is characterized in that, described Maca functional drink also comprises the component of following mass ratio:
HFCS 2% ~ 6%, citric acid 0.04% ~ 0.07%, xanthans 0.01% ~ 0.07%.
9. according to the Maca functional drink in claim 6 ~ 8 described in any one, it is characterized in that, described Maca functional drink is sterilizing 4 ~ 15S at 135 ~ 150 DEG C, and sterile filling after cooling, carries out spray sterilizing after filling, get product.
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