CN105557638A - Method for culturing earthworms for infusing fructus lycii, salvia miltiorrhiza and earthworm wine and application of earthworms - Google Patents
Method for culturing earthworms for infusing fructus lycii, salvia miltiorrhiza and earthworm wine and application of earthworms Download PDFInfo
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Abstract
The invention provides a method for culturing earthworms for infusing fructus lycii, salvia miltiorrhiza and earthworm wine and application of the earthworms. The method for culturing the earthworms comprises the steps of separately preparing fructus lycii powder, salvia miltiorrhiza powder and fermented wheat bran for culturing the earthworms, and removing internal dirt from the earthworms, thereby obtaining a purified product. A method for infusing the fructus lycii, salvia miltiorrhiza and earthworm wine comprises the step of infusing the purified product with 60-degree pure-grain liquor. The weight percent of earthworm protein in the fructus lycii, salvia miltiorrhiza and earthworm wine is 25.5% to 27.6%. By adopting fructus lycii and salvia miltiorrhiza to feed the earthworms as auxiliaries and then infusing the earthworms, the disadvantage that earthworm wine produced by adopting the conventional infusing methods has a rich offensive smell and is poor in taste is effectively eliminated, and the mind of medicine taking contradiction of patients is lowered. Already-crushed fructus lycii and salvia miltiorrhiza are added in stages during culturing, then, the earthworms gain the capacity for gradually accommodating to mixed feedstuff, the phenomenon of anorexia of the earthworms is eliminated, the cultured earthworms are big and fleshy in physical constitution, the culture cycle is short, and the protein content of the earthworms is high.
Description
Technical field
The invention belongs to earthworm (Earthworm) and cultivate field, particularly relate to cultural method and the application of a kind of earthworm for the brewed fruit of Chinese wolfberry, red sage root earthworm liquor.
Background technology
" earthworm liquor " be with fresh earthworm for primary raw material, add Chinese herbal medicine and good quality propagation wine through meticulously formulated, have and relax the muscles and stimulate the blood circulation, dispelling, collateral-activating, exempt from numbness dehumidifying, liver-kidney tonifying, the effect such as strengthen muscles and bones.Treat chill, heat, arthritis with fixed pain caused by dampness, lumbocrural pain, the contraction of muscle arteries and veins, redness and swelling of joints, stretch the various disease conditions such as Qu Buli, apoplexy and hemiplegia, larynx numbness, bleeding from the gum, urinary obstruction, scrofula, sore, become one of important medicinal material in Chinese medicine.
The ripening fruits that the fruit of Chinese wolfberry (FRUCTUSLYCII) is matrimony vine of solanaceae plant or lycium barbarum.Pluck when summer, autumn fruit maturation, removing carpopodium, put after shady and cool place dries in the air and corrugate to pericarp, then be exposed to the sun stiff to crust, pulp is soft and get final product.Containing 14 seed amino acids in the fruit of Chinese wolfberry, and containing special dietary compositions such as betain, beautiful another name for Sichuan Province flavine, physalins, there is outstanding health-care efficacy.Cure mainly nourishing liver and kidney, benefiting shrewd head.Lose for consumptive disease essence, soreness of waist and knee joint, dizzy tinnitus, heat are quenched one's thirst, and the deficiency of blood is sallow, and blurred vision is failed to understand.
The red sage root (SalviamiltiorrhizaBunge), root is used as medicine, and containing tanshinone, is robustness emmenagogue, the stasis of blood of dispelling, raw new, invigorate blood circulation, the effectiveness such as menstruation regulating, be gynaecology's key medicine, cure mainly uterine hemorrhage, irregular menstruation, blood stasis, stomachache, dysmenorrhoea, through closing, mausoleum pain.Good result is had to treatment coronary heart disease.In addition also control the weak insomnia of nerve, arthralgia, anaemia, mastitis, lymphadenitis, arthritis, sore furuncle pain is swollen, erysipelas, acute, chronic hepatitis, nephropyelitis, traumatic injury, advanced schistosomiasis hepatosplenomegaly, epilepsy.
Medicinal liquor is a kind of leaching preparation, and the preparation method of traditional medicinal liquor is that Chinese herbal medicine is brewed in wine according to a certain percentage, the desorb function utilizing ethanol good, dissolves the active ingredient in Chinese herbal medicine.After alcohol has dissolved a lot of material in cell, Intracellular solution concentration is made to be significantly higher than extracellular and to form concentration difference, utilize this concentration difference, intracellular high concentration leachate is constantly spread to the extracellular in low concentration direction, the soluble component in medicine is dissolved in wine gradually.The fruit of Chinese wolfberry, red sage root earthworm liquor are a kind of eutherapeutic medicinal liquor, tonifying middle-Jiao and Qi, treat fatigue and asthenia, soreness of waist and knee joint, stretch Qu Buli.
The cultivation of current earthworm, the main wheat bran that adopts is as feed, and the culture-cycle is long, earthworm poor quality.As the Authorization Notice No. Chinese patent that is CN102524192B discloses the cultural method of a kind of edible earthworm, it is characterized in that, comprise the following steps: get 45 ~ 65 parts of rice brans, 20 ~ 28 parts of powder of straw or straw sawdust, 4 ~ 8 parts of wheat bran, 4 ~ 7 parts of fish meal, 5 ~ 10 parts of dregs of beans, 1 ~ 3 part of anaerobic bacteria are thoroughly mixed to form fermentation heap and carry out anaerobic fermentation, obtain earthworm feedstuff; Earthworm feedstuff is put into culturing pool or breeding box, and put into earthworm kind and cultivate.The earthworm these class methods cultivated carries out the brewed of earthworm liquor, and earthworm liquor protein content is low; Mouthfeel is poor, has fishy smell, is difficult to swallow, and it is serious that patient takes psychology of conflicting, and result for the treatment of is poor.
Summary of the invention
For solving the problem, the invention provides cultural method and the application of a kind of earthworm for the brewed fruit of Chinese wolfberry, red sage root earthworm liquor.
For a cultural method for the earthworm of the brewed fruit of Chinese wolfberry, red sage root earthworm liquor, it is characterized in that: comprise the following steps:
1) prepare the fruit of Chinese wolfberry, red sage root powder: take the fruit of Chinese wolfberry by 1 weight portion and drier put into by the 0.2-12 weight portion red sage root, when being 3%-5% to the moisture in the fruit of Chinese wolfberry, the red sage root, take out; The fruit of Chinese wolfberry of taking-up, the red sage root are placed in continuous flow cracker, are crushed to fineness 74-124 μm, for subsequent use;
2) fermenting wheat bran is prepared: take wheat bran and 0.006-0.013 weight portion BGB microbial bacterial agent by 3 weight portions, add distilled water 2-3 part after mixing wherein, stir while add waterside, feed is placed in fermentation tank, at 20-30 DEG C of bottom fermentation 10-15 hour, the wheat bran of obtained fermentation;
3) cultivate: take the step 2 that parts by weight are 3.5 parts) add 0.3-1.2 weight portion the step 1) obtained fruit of Chinese wolfberry, red sage root powder in obtained fermenting wheat bran, drop in wooden breeding box, the earthworm adding 1 part of parts by weight cultivates, control cultivation temperature 15 DEG C-25 DEG C, the moisture of spray control feed, at 20-30%, cultivates 4 ~ 6 days;
4) earthworm after step 3) being cultivated is sieved, obtain earthworm and screening reject, breeding box is put back to after weighing the weight of screening reject, the fruit of Chinese wolfberry, the red sage root powder of 1/2 screening reject weight is added again in breeding box, upset breeding box, makes powder mix, and the earthworm after sieving is put into breeding box, the moisture of spray control feed, at 20-30%, cultivates 9 ~ 12 days;
5) earthworm after step 4) being cultivated is sieved, obtain earthworm and screening reject, breeding box is put back to after weighing the weight of screening reject, in breeding box, add the fruit of Chinese wolfberry, the red sage root powder of 1/2 screening reject weight again, upset breeding box, makes powder mix, and the earthworm after sieving is put into breeding box, the moisture of spray control feed, at 20-30%, by vermiculture 12 ~ 17 days, obtains the earthworm primary product of the brewed fruit of Chinese wolfberry, red sage root earthworm liquor;
6) the brewed fruit of Chinese wolfberry described in, the earthworm primary product of red sage root earthworm liquor put into the container that wet toilet paper is housed, and leave standstill cultivation 1 day, remove the foul in earthworm body, are purified product.
Described BGB microbial bacterial agent is that Beijing Jiabowen Bioscience Co., Ltd. produces, the content of organic matter >=40%; Living bacteria count >=200,000,000 cfu/g; Every mu of consumption: 20-80 kg/acre, meets national agricultural microbial agent standard GB20287-2006.
A method for the brewed fruit of Chinese wolfberry, red sage root earthworm liquor, is characterized in that, comprise the following steps:
1) the purification product after claim 1 being cultivated take out, clean with cleaning machine, the earthworm weighing 1 part of weight puts into clean soaking container, add the pure grain wine of 60 ° of 5 parts of weight, mixing shakes up rear covered container lid, soak 30 days at 20-30 DEG C, active ingredient is fully leached, and in immersion process, every day stirs twice;
2) pull the earthworm in wine after having soaked out, filter the flocculent deposit of removing in earthworm wine, obtain clarification earthworm wine, then the earthworm wine of clarification is distributed into bottle storage.
A kind of fruit of Chinese wolfberry, red sage root earthworm liquor, adopt said method to make, in the described fruit of Chinese wolfberry, red sage root earthworm liquor the weight percent content of dragon protein be 25.5%-27.6%.
Beneficial effect of the present invention is: 1) by the earthworm adopting the fruit of Chinese wolfberry, the red sage root is fed as auxiliary material, again this kind of earthworm is carried out brewed, effectively eliminate the shortcoming that the earthworm liquor adopting conventional brewing method to produce has strong fishy smell, mouthfeel difference, reduce the conflict psychology that patient takes medicine.2) due to conventional feed that the fruit of Chinese wolfberry, the red sage root are not earthworm, if be directly added to feed ingredient, the food-intake of easy reduction earthworm, earthworm rugosity can be caused little, by wheat bran BGB microbial bacterial agent is fermented, make bait micro-sweet after, food-intake can be improved, and by breeding process, add the fruit of Chinese wolfberry and the red sage root pulverized stage by stage, make earthworm produce an ability adapted to gradually to the fruit of Chinese wolfberry, red sage root mixed feed, not only eliminate the phenomenon of the apocleisis of earthworm, the earthworm physique of cultivation is big and fleshy, cultivation cycle is short, and earthworm protein content is high; 3) by the fruit of Chinese wolfberry, red sage root smashing fineness 74-124 μm, make powder enough little be bonded at wheat bran, add the uniformity that wheat bran mixes with the fruit of Chinese wolfberry, the red sage root, make bait more easily be eaten by earthworm simultaneously; 4) only select the fruit of Chinese wolfberry, the red sage root and wheat bran as raw material, component is determined, cost is low, and for the cultivation of earthworm and the brewed of medicinal liquor, constituent content is more controlled.
Embodiment
Explanation is further explained to the present invention below.
Use the Pheretima aspergillum propagated artificially in this experiment, have another name called LUMBRICUS, initially degree of enclosing is 0.15-0.16cm.
control group
1) fermenting wheat bran is prepared: the preparation method of described fermenting wheat bran is for taking wheat bran 3kg, BGB microbial bacterial agent 0.009kg, distilled water 2kg is added wherein after mixing, stir while add waterside, can not scatter after holding with a firm grip, feed is placed in fermentation tank, 20-30 DEG C of bottom fermentation 12 hours, the wheat bran of obtained fermentation;
2) cultivate: the fermenting wheat bran 3.5kg obtained by step 1), drop in wooden breeding box, the earthworm adding 1kg cultivates, and control cultivation temperature 15 DEG C-25 DEG C, the moisture of spray control feed, at about 20-30%, cultivates 5 days;
3) by step 2) cultivation after earthworm sieve, obtain earthworm and screening reject, second time measures earthworm degree of enclosing, screening reject is put back in breeding box, upset breeding box, makes powder mix, then the earthworm after sieving is put into breeding box, the moisture of spray control feed, at about 20-30%, cultivates 10 days;
4) earthworm after step 3) being cultivated is sieved, obtain earthworm and screening reject, third time measures earthworm degree of enclosing, and is put back to by screening reject in breeding box, upset breeding box, powder is mixed, again the earthworm after sieving is put into breeding box, the moisture of spray control feed at about 20-30%, by vermiculture 15 days, sieve, measure earthworm degree of enclosing the 4th time;
5) earthworm after cultivation puts into the container that wet toilet paper is housed, and leaves standstill cultivation 1 day, removes the foul in earthworm body;
6) earthworm of taking out step 5) obtained is cleaned with cleaning machine, takes 1kg and puts into clean soaking container, add the pure grain wine 5kg of 60 °, mixing shakes up rear covered container lid, soak 30 days at 20-30 DEG C, active ingredient is fully leached, and in immersion process, every day stirs twice;
7) pull the earthworm in wine after having soaked out, filter the flocculent deposit of removing in earthworm wine, obtain clarification earthworm wine, then the earthworm wine of clarification is distributed into bottle storage.
Wherein BGB microbial bacterial agent is composited by actinomycetes, bacillus, azotobacter, organic phosphobacteria, inorganic phosphorus bacteria, B. mucilaginocus, lactic acid bacteria and saccharomycete, and manufacturer is Beijing Jiabowen Bioscience Co., Ltd..
embodiment 1
1) prepare the fruit of Chinese wolfberry, red sage root powder: take the 1kg fruit of Chinese wolfberry and the 1.5kg red sage root, put into drier, when being 3%-5% to the moisture in the fruit of Chinese wolfberry, the red sage root, take out; Take the fruit of Chinese wolfberry, the red sage root is placed in continuous flow cracker, the fruit of Chinese wolfberry, danshen powder are broken to fineness 74-124 μm, for subsequent use;
2) prepare fermenting wheat bran: take wheat bran 3kg, BGB microbial bacterial agent 0.009kg, adds distilled water 2kg after mixing wherein, stirs, be placed in by feed in fermentation tank while add waterside, 20-30 DEG C of bottom fermentation 12 hours, and the wheat bran of obtained fermentation;
3) cultivate: take step 2) add 1.0kg the step 1) obtained fruit of Chinese wolfberry, red sage root powder in obtained 3.5kg fermenting wheat bran, drop in wooden breeding box, the earthworm adding 1kg cultivates, and controls cultivation temperature 15 DEG C-25 DEG C, the moisture controlling feed, at 20-30%, cultivates 5 days;
4) earthworm after step 3) being cultivated is sieved, obtain earthworm and screening reject, second time measures earthworm degree of enclosing, and the weight weighing screening reject is 3.2kg, screening reject is put back to breeding box and in breeding box, adds the fruit of Chinese wolfberry, red sage root powder 1.6kg, upset breeding box, powder is mixed, and the earthworm after sieving is put into breeding box, watering, the moisture controlling feed, at 20-30%, cultivates 10 days;
5) earthworm after step 4) being cultivated is sieved, and obtains earthworm and screening reject, and third time measures earthworm degree of enclosing, the weight weighing screening reject is 3.6kg, screening reject is put back to breeding box and in breeding box, adds the fruit of Chinese wolfberry, red sage root powder 1.8kg, upset breeding box, makes powder mix, and the earthworm after sieving is put into breeding box, watering in two days once, controlled the moisture of feed at 20-30%, by vermiculture 15 days, sieve, measure earthworm degree of enclosing the 4th time; Observe color and luster;
6) earthworm after cultivation puts into the container that wet toilet paper is housed, and leaves standstill cultivation 1 day, removes the foul in earthworm body;
7) earthworm that taking-up step 6) is obtained takes earthworm 1kg and puts into clean soaking container, add the pure grain wine 5kg of 60 ° after cleaning, mixing shakes up rear covered container lid, soak 30 days at 20-30 DEG C, active ingredient is fully leached, and in immersion process, every day stirs twice;
8) pull the earthworm in wine after having soaked out, filter the flocculent deposit of removing in earthworm wine, obtain clarification earthworm wine, then the earthworm wine of clarification is distributed into bottle storage.
Wherein BGB microbial bacterial agent is composited by actinomycetes, bacillus, azotobacter, organic phosphobacteria, inorganic phosphorus bacteria, B. mucilaginocus, lactic acid bacteria and saccharomycete, and manufacturer is Beijing Jiabowen Bioscience Co., Ltd..
embodiment 2
1) prepare the fruit of Chinese wolfberry, red sage root powder: take the 1kg fruit of Chinese wolfberry and the 0.2kg red sage root, put into drier, when being 3%-5% to the moisture in the fruit of Chinese wolfberry, the red sage root, take out; Take the fruit of Chinese wolfberry, the red sage root is placed in continuous flow cracker, the fruit of Chinese wolfberry, danshen powder are broken to fineness 74-124 μm, for subsequent use;
2) prepare fermenting wheat bran: take wheat bran 3kg, BGB microbial bacterial agent 0.006kg, adds distilled water 2kg after mixing wherein, stirs, be placed in by feed in fermentation tank while add waterside, 20-30 DEG C of bottom fermentation 10 hours, and the wheat bran of obtained fermentation;
3) step 2 is taken) obtained fermenting wheat bran 3.5kg, add the fruit of Chinese wolfberry, red sage root powder that 0.3kg step 1) is obtained, drop in wooden breeding box, the earthworm adding 1kg cultivates, control cultivation temperature 15 DEG C-25 DEG C, the moisture controlling feed, at 20-30%, cultivates 4 days;
4) earthworm after step 3) being cultivated is sieved, obtain earthworm and screening reject, second time measures earthworm degree of enclosing, the weight weighing screening reject is that 3.4kg puts back to breeding box and in breeding box, adds the fruit of Chinese wolfberry, red sage root powder 1.7kg, and upset breeding box, makes powder mix, and the earthworm after sieving is put into breeding box, watering, the moisture controlling feed, at 20-30%, cultivates 9 days;
5) earthworm after step 4) being cultivated is sieved, obtain earthworm and screening reject, third time measures earthworm degree of enclosing, and the weight weighing screening reject is that 4.2kg puts back to breeding box and in breeding box, adds the fruit of Chinese wolfberry, red sage root powder 2.1kg, upset breeding box, powder is mixed, and the earthworm after sieving is put into breeding box, watering in two days once, control the moisture of feed at 20-30%, by vermiculture 12 days, sieve, measure earthworm degree of enclosing the 4th time; Observe color and luster;
6) earthworm after cultivation puts into the container that wet toilet paper is housed, and leaves standstill cultivation 1 day, removes the foul in earthworm body;
7) earthworm that taking-up step 6) is obtained takes 1kg and puts into clean soaking container, add the pure grain wine 5kg of 60 ° after cleaning with cleaning machine, mixing shakes up rear covered container lid, soak 30 days at 20-30 DEG C, active ingredient is fully leached, and in immersion process, every day stirs twice;
8) pull the earthworm in wine after having soaked out, filter the flocculent deposit of removing in earthworm wine, obtain clarification earthworm wine, then the earthworm wine of clarification is distributed into bottle storage.
Wherein BGB microbial bacterial agent is composited by actinomycetes, bacillus, azotobacter, organic phosphobacteria, inorganic phosphorus bacteria, B. mucilaginocus, lactic acid bacteria and saccharomycete, and manufacturer is Beijing Jiabowen Bioscience Co., Ltd..
embodiment 3
1) prepare the fruit of Chinese wolfberry, red sage root powder: take the 1kg fruit of Chinese wolfberry and the 12kg red sage root, put into drier, when being 3%-5% to the moisture in the fruit of Chinese wolfberry, the red sage root, take out; Take the fruit of Chinese wolfberry, the red sage root is placed in Continuous Flow cracker, the fruit of Chinese wolfberry, danshen powder are broken to fineness 74-124 μm, for subsequent use;
2) prepare fermenting wheat bran: take wheat bran 3kg, BGB microbial bacterial agent 0.013kg, adds distilled water 3kg after mixing wherein, stirs, be placed in by feed in fermentation tank while add waterside, 20-30 DEG C of bottom fermentation 15 hours, and the wheat bran of obtained fermentation;
3) cultivate: take step 2) obtained fermenting wheat bran 3.5kg, add the fruit of Chinese wolfberry, red sage root powder that 1.2kg step 1) is obtained, drop in wooden breeding box, the earthworm adding 1kg cultivates, control cultivation temperature 15 DEG C-25 DEG C, the moisture controlling feed, at 20-30%, cultivates 6 days;
4) earthworm after step 3) being cultivated is sieved, obtain earthworm and screening reject, second time measures earthworm degree of enclosing, and weighs the weight 3.6kg of screening reject, screening reject is put back to breeding box and in breeding box, adds the fruit of Chinese wolfberry, red sage root powder 1.8kg, upset breeding box, powder is mixed, and the earthworm after sieving is put into breeding box, watering, the moisture controlling feed, at 20-30%, cultivates 12 days;
5) earthworm after step 4) being cultivated is sieved, and obtains earthworm and screening reject, and third time measures earthworm degree of enclosing, weigh the weight 4.4kg of screening reject, screening reject is put back to breeding box and in breeding box, adds the fruit of Chinese wolfberry, red sage root powder 2.2kg, upset breeding box, makes powder mix, and the earthworm after sieving is put into breeding box, watering in two days once, controlled the moisture of feed at 20-30%, by vermiculture 17 days, sieve, measure earthworm degree of enclosing the 4th time; Observe color and luster;
6) earthworm after cultivation puts into the container that wet toilet paper is housed, and leaves standstill cultivation 1 day, removes the foul in earthworm body;
7) earthworm that taking-up step 6) is obtained takes 1kg and puts into clean soaking container, add the pure grain wine 5kg of 60 ° after cleaning with cleaning machine, mixing shakes up rear covered container lid, soak 30 days at 20-30 DEG C, active ingredient is fully leached, and in immersion process, every day stirs twice;
8) pull the earthworm in wine after having soaked out, filter the flocculent deposit of removing in earthworm wine, obtain clarification earthworm wine, then the earthworm wine of clarification is distributed into bottle storage.
Wherein BGB microbial bacterial agent is composited by actinomycetes, bacillus, azotobacter, organic phosphobacteria, inorganic phosphorus bacteria, B. mucilaginocus, lactic acid bacteria and saccharomycete, and manufacturer is Beijing Jiabowen Bioscience Co., Ltd..
experimental result detects
Medicinal liquor after brewed is carried out to the detection of active ingredient ground dragon protein, adopt Coomassie Brilliant Blue, Coomassie brilliant G-250 (for Amereaco company), with bovine serum albumin (sigma, A7030) be reference substance, utilize 745 type ultraviolet-visible spectrophotometers (Shanghai Spectrum Apparatus Co., Ltd.), the gradient configuration of reference substance adopts the pure grain wine of 60 °, under 595nm wavelength, by the protein yield that converts to obtain.
Experimental result
Experimental result shows to add stage by stage the fruit of Chinese wolfberry, the red sage root is fed compared with earthworm feeds earthworm with direct wheat bran, and earthworm degree of enclosing increases rapid, and carry out the brewed of earthworm liquor, earthworm protein content is high; Because earthworm growth is fast, therefore culturing time is short; Due to the unconventional bait that the fruit of Chinese wolfberry, the red sage root are earthworm, adopt the raising method adding the fruit of Chinese wolfberry, the red sage root stage by stage, earthworm has a process slowly adapted to the fruit of Chinese wolfberry, the red sage root, more contributes to the cultivation of excellent earthworm.
The clinical observation of the arthralgia of rheumatic disease
Physical data
That randomly draws 18-50 year suffers from rheumatic disease and with the individuality 60 of arthralgia, is divided into three groups at random, numbering A, B, C.
Diagnostic criteria
The clinical manifestation of rheumatic disease is that: Tou Tong ﹑ Wei Han ﹑ Shen Chong ﹑ joint Suan Tong ﹑ can not bend and stretch.May, with swelling and moving obstacle, be also outbreak and the alleviation chronic course of disease alternately.
Methods for the treatment of
A, B component are not taken the earthworm liquor that embodiment 1, control group 1 are obtained, take to the 3rd group of (C group) patient, 60 ° of pure grain wines, sooner or later each once, each 3mL was a course for the treatment of with 10 days, after treating two courses for the treatment of, followed up a case by regular visits to half a year, evaluated.
Treatment evaluation criteria
Recovery from illness: after treating 2 courses for the treatment of, above-mentioned clinical symptoms disappears, follows up a case by regular visits in half a year without recurrence;
Take a turn for the better: after treating 2 courses for the treatment of, above-mentioned clinical symptoms is obviously improved, progressively recover normal;
Invalid: after treating 2 courses for the treatment of, above-mentioned clinical part symptom, without change, does not reach improvement standard person;
Efficient=(recovery from illness number+good revolution) * 100%/case load.
。
As can be seen here, the fruit of Chinese wolfberry brewed in embodiment 1, red sage root earthworm liquor are applied to clinical by the present invention, experimental result shows, adopt the earthworm that feed formula of the present invention cultivates, the brewed fruit of Chinese wolfberry, red sage root earthworm liquor, clinical treatment good effect, efficient high with cure rate, and the fruit of Chinese wolfberry, red sage root earthworm liquor do not have fishy smell, and mouthfeel is good, patient treatment is conflicted psychological little.
Above embodiments of the invention have been described in detail, but described content being only preferred embodiment of the present invention, can not being considered to for limiting practical range of the present invention.All equalizations done according to the present patent application scope change and improve, and all should belong within patent covering scope of the present invention.
Claims (4)
1., for a cultural method for the earthworm of the brewed fruit of Chinese wolfberry, red sage root earthworm liquor, it is characterized in that: comprise the following steps:
1) prepare the fruit of Chinese wolfberry, red sage root powder: take the fruit of Chinese wolfberry by 1 weight portion and drier put into by the 0.2-12 weight portion red sage root, when being 3%-5% to the moisture in the fruit of Chinese wolfberry, the red sage root, take out; The fruit of Chinese wolfberry of taking-up, the red sage root are placed in continuous flow cracker, are crushed to fineness 74-124 μm, for subsequent use;
2) fermenting wheat bran is prepared: take wheat bran and 0.006-0.013 weight portion BGB microbial bacterial agent by 3 weight portions, add distilled water 2-3 part after mixing wherein, stir while add waterside, feed is placed in fermentation tank, at 20-30 DEG C of bottom fermentation 10-15 hour, the wheat bran of obtained fermentation;
3) cultivate: take the step 2 that parts by weight are 3.5 parts) add 0.3-1.2 weight portion the step 1) obtained fruit of Chinese wolfberry, red sage root powder in obtained fermenting wheat bran, drop in wooden breeding box, the earthworm adding 1 part of parts by weight cultivates, control cultivation temperature 15 DEG C-25 DEG C, the moisture of spray control feed, at 20-30%, cultivates 4 ~ 6 days;
4) earthworm after step 3) being cultivated is sieved, obtain earthworm and screening reject, breeding box is put back to after weighing the weight of screening reject, the fruit of Chinese wolfberry, the red sage root powder of 1/2 screening reject weight is added again in breeding box, upset breeding box, makes powder mix, and the earthworm after sieving is put into breeding box, the moisture of spray control feed, at 20-30%, cultivates 9 ~ 12 days;
5) earthworm after step 4) being cultivated is sieved, obtain earthworm and screening reject, breeding box is put back to after weighing the weight of screening reject, in breeding box, add the fruit of Chinese wolfberry, the red sage root powder of 1/2 screening reject weight again, upset breeding box, makes powder mix, and the earthworm after sieving is put into breeding box, the moisture of spray control feed, at 20-30%, by vermiculture 12 ~ 17 days, obtains the earthworm primary product of the brewed fruit of Chinese wolfberry, red sage root earthworm liquor;
6) the brewed fruit of Chinese wolfberry described in, the earthworm primary product of red sage root earthworm liquor put into the container that wet toilet paper is housed, and leave standstill cultivation 1 day, remove the foul in earthworm body, are purified product.
2. the cultural method of a kind of earthworm for the brewed fruit of Chinese wolfberry, red sage root earthworm liquor as claimed in claim 1, is characterized in that described BGB microbial bacterial agent is that Beijing Jiabowen Bioscience Co., Ltd. produces, the content of organic matter >=40%; Living bacteria count >=200,000,000 cfu/g; Every mu of consumption: 20-80 kg/acre, meets national agricultural microbial agent standard GB20287-2006.
3. a method for the brewed fruit of Chinese wolfberry, red sage root earthworm liquor, is characterized in that, comprise the following steps:
1) the purification product after claim 1 being cultivated take out, clean with cleaning machine, the earthworm weighing 1 part of weight puts into clean soaking container, add the pure grain wine of 60 ° of 5 parts of weight, mixing shakes up rear covered container lid, soak 30 days at 20-30 DEG C, active ingredient is fully leached, and in immersion process, every day stirs twice;
2) pull the earthworm in wine after having soaked out, filter the flocculent deposit of removing in earthworm wine, obtain clarification earthworm wine, then the earthworm wine of clarification is distributed into bottle storage.
4. the fruit of Chinese wolfberry, a red sage root earthworm liquor, is characterized in that: adopt said method to make, in the described fruit of Chinese wolfberry, red sage root earthworm liquor the weight percent content of dragon protein be 25.5%-27.6%.
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| CN107125447A (en) * | 2017-05-24 | 2017-09-05 | 杜健敏 | A kind of earthworm feedstuff |
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| CN107125447A (en) * | 2017-05-24 | 2017-09-05 | 杜健敏 | A kind of earthworm feedstuff |
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